Chlorine-treated Water Research Articles

Geographical Origin, WASH Access, and Clinical Descriptions for Patients Admitted to a Cholera Treatment Center in Northwest Syria between October and December 2022.

Background On September 10, 2022, a cholera outbreak was declared in Syria for the first time in over a decade of protracted conflict. As of May 20, 2023, 132,782 suspected cases had been reported, primarily in northwest and northeast Syria. We aim to provide a detailed description of water sources and clinical status of a patient cohort seen at a cholera treatment center (CTC) in northwest Syria. Methods We retrospectively identified patients with confirmed cholera who presented to the CTC in Idlib governorate between October 8 and December 18, 2022. Data were obtained from clinical case records and analyzed in R v4.0.4. Results Ninety-four patients (55.3% men) were treated at the CTC. Thirty-five patients were severely dehydrated (Plan C treatment), 54 had some dehydration (Plan B), and 5 had no dehydration (Plan A). Most patients were between 11 and 20 years old ( n = 25, 26.6%) or 31 and 40 years old ( n = 19, 20.2%). Note that 70.2% ( n = 66) of patients were seen in November 2022 and most were from Harim district ( n = 44, 46.8%). Public wells ( n = 46, 48.9%) and water trucking ( n = 41, 43.6%) were the most commonly used water sources. Note that 76.6% ( n = 72) did not have access to chlorine-treated water. Forty-seven patients (50%) had more than five water, sanitation, and hygiene (WASH)-related cholera risk factors. Following treatment, six patients were transferred to another treatment center, three died (case fatality rate: 3.2%), and the remainder were discharged. Conclusion Most patients reported WASH-related risk factors for cholera, reflecting the poor state of WASH in northwest Syria after over a decade of conflict. This relates to the direct and indirect impacts of urban and periurban violence as well as the underfunded humanitarian response. Strengthening WASH and health promotion are important components to control the outbreak.

Application of Prussian Blue in Electrochemical and Optical Sensing of Free Chlorine.

In this paper, an electrochemical free chlorine (FCL) sensor was formed by modifying a fluorine-doped tin oxide-coated glass slide (glass|FTO) with a layer of Prussian blue (glass|FTO|PB). The glass|FTO|PB sensor exhibited a wide linear detection range from 1.7 to 99.2 μmol L−1 of FCL with a sensitivity of ~0.8 µA cm−2 μmol−1 L and showed high selectivity for FCL. However, , and ions have induced only a negligible amperometric response that is highly beneficial for a real-life sample analysis as these ions are commonly found in chlorine-treated water. Moreover, in this work, optical absorption measurement-based investigations of partially reduced PB were carried out as a means to characterize PB catalytic activity towards FCL and to investigate the possibility of applying PB for the optical detection of FCL.

Experimental study on total coliform violations in the complied NH2CL, O3, and UV treated municipal water supply system.

Water quality has become a severe concern on a global scale, owing mostly to the rapid increase of the nation's development. According to Malaysia's Natural Resources and Environment Ministry, poor water management is the primary cause of the country's water quality problems. Many river systems are polluted by home and industrial pollutants, according to the findings of research in Malaysia and comparable difficulties in a few other nations. Hence, the following are the research's goals: (1) To look into what is causing the infractions. (2) To undertake the inquiry, develop a thorough hypothesis. (3) To detect dangerous germs by sampling the most usually infected regions. (4) To develop a test for Total Coliform violations in chlorine-treated water at the water treatment plant and in water distribution systems. As a result, the most major barrier to ensuring the safe delivery of treated water to consumers and protecting human health from water-related diseases is the drinking water treatment process. As a result, practically all water treatment systems around the world, including those in the USA, use a chlorine-based procedure to disinfect the water system during treatment. According to studies, the ideal way of disinfecting treated water is both safe and beneficial. Any sort of pandemic or biologically caused disease has no societal implications. Many countries began to suffer in 2009 as a result of e-coli and total coliform contamination in their water systems, leading to ambiguity in disinfection methods. Some water from UNMC's coolers was within the guidelines, while some exceed them. Water coolers at Block E (614 m) and Block B (605 m), for example, measured 12 CFU/100 ml and 11 CFU/100 ml, respectively. Water coolers should be cleaned regularly to ensure that they perform correctly. Further, the microbial population was found to be higher at water storage tanks than that is at the water cooler. This demonstrates how a water cooler fulfils its purpose of filtering and trapping germs to provide clean drinking water.Graphical abstract

Comparison of disinfectants for drinking water: chlorine gas vs. on-site generated chlorine

The feasibility of on-site generated chlorine (OSG chlorine) as an alternative disinfectant to chlorine gas was evaluated in terms of total organic carbon (TOC) removal, disinfection efficiency, biofilm control, disinfection by-products (DBPs) formation, chlorine decay rate, and volatility. Chlorine gas decreased the pH of the treated water by -0.1 per mg/L of free available chlorine (FAC) while OSG chlorine increased the pH by + 0.06 per mg/L. OSG chlorine with more hypochlorite ion (OCl-) at higher pH was less effective in the inactivation of suspended bacteria and TOC removal but remained in the distribution system longer and controlled biofilm formation more effectively than chlorine gas. The DBPs formation by OSG chlorine was not significantly different from that by chlorine gas except for the reduction of Haloacetonitriles. Hypochlorous acid (HOCl) was more volatile than OCl-, indicating the lower volatility of FAC in the OSG chlorine-treated water. Two types of OSG systems, “Mixed oxidants” and OSG hypochlorite, did not show any significant difference in disinfection, DBPs formation, and chlorine decay rate (paired t-test, p = 0.40, 0.11 ~ 0.70, > 0.42). A significant synergy effect by oxidants other than FAC cannot be expected in the use of “mixed oxidants” at a water treatment plant.

Heat and chlorine resistance of a soil Acanthamoeba sp. cysts in water.

The study established the inactivation kinetic parameters of an Acanthamoeba cyst isolate subjected to heating and chlorination. A strain of Acanthamoeba was isolated and purified from an area surrounding a pilot food plant. Mature cysts (14days) were subjected to heat inactivation studies at 71, 76, 81, 86 and 91°C; and chlorination at 100, 200, 300, 400 and 500ppm. The decimal reduction times (D-values) at 71, 76, 81, 86 and 91°C were 18·31, 9·26, 7·35, 4·52 and 1·81min respectively. The calculated thermal resistance constant (z-value) was 21·32°C (R2 =0·96-0·97). The D-value in 100, 200, 300, 400 and 500ppm chlorine-treated water were 47·17, 25·06, 24·51, 23·70 and 18·55min respectively. The chlorine resistance constant (z-value) was 1179ppm chlorine (R2 =0·65-0·74). Results demonstrated high resistance of the isolated Acanthamoeba cysts towards the common methods applied in ensuring food and food processing environment sanitation. The resistance parameters of the test organisms established in this study may be used in the establishment of Sanitation Standard Operating Procedures (SSOPs), which are often based on inactivation of bacteria. These SSOPs could render better protection to food and food processing environments.

Simultaneous analysis of 11 haloacetic acids by direct injection-liquid chromatography-electrospray ionization-triple quadrupole tandem mass spectrometry and high resolution mass spectrometry: occurrence and evolution in chlorine-treated water.

A fast, simple, selective, and sensitive method for the analysis of 11 haloacetic acids (HAAs) in chlorine-treated water has been developed. The method is based on liquid chromatography-electrospray ionization-triple quadrupole tandem mass spectrometry (LC/ESI-QqQ-MS/MS) with direct injection of the aqueous sample. The main novelty of this method over the previously published procedures based on different techniques of mass spectrometry with direct injection is the combination of the simultaneous analysis of three types of HAAs (chlorinated, brominated, and iodinated) with its simplicity and low LODs (0.01-0.6μg/L), avoiding the use of ion-pairing reagents for LC as well as the complexity and high cost of other techniques such as ion chromatography and capillary electrophoresis coupled to tandem mass spectrometry (IC-MS/MS and CE-MS/MS). The developed method was compared with another procedure carried out in our laboratory based on direct injection-liquid chromatography-electrospray ionization-high-resolution mass spectrometry with an Orbitrap analyzer (LC/ESI-Orbitrap-HRMS). The application of this technique to HAA analysis had not been previously described. LODs achieved by LC-HRMS (0.01-2μg/L) were higher than the ones obtained by LC-MS/MS. Therefore, the LC/ESI-QqQ-MS/MS method was applied to the analysis of real samples. Quality parameters were calculated with satisfactory results and real samples related to three drinking water treatment plants (DWTPs), tap water, and the drinking water distribution system of Barcelona area (Catalonia, NE Spain) were analyzed. Furthermore, the evolution of HAA concentration along time in a DWTP-treated water sample was studied.

Achieving optimal technology and behavioral uptake of single and combined interventions of water, sanitation hygiene and nutrition, in an efficacy trial (WASH benefits) in rural Bangladesh

BackgroundUptake matters for evaluating the health impact of water, sanitation and hygiene (WASH) interventions. Many large-scale WASH interventions have been plagued by low uptake. For the WASH Benefits Bangladesh efficacy trial, high uptake was a prerequisite. We assessed the degree of technology and behavioral uptake among participants in the trial, as part of a three-paper series on WASH Benefits Intervention Delivery and Performance.MethodsThis study is a cluster randomized trial comprised of geographically matched clusters among four districts in rural Bangladesh. We randomly allocated 720 clusters of 5551 pregnant women to individual or combined water, sanitation, handwashing, and nutrition interventions, or a control group. Behavioral objectives included; drinking chlorine-treated, safely stored water; use of a hygienic latrine and safe feces disposal at the compound level; handwashing with soap at key times; and age-appropriate nutrition behaviors (pregnancy to 24 months) including a lipid-based nutrition supplement (LNS). Enabling technologies and behavior change were promoted by trained local community health workers through periodic household visits. To monitor technology and behavioral uptake, we conducted surveys and spot checks in 30–35 households per intervention arm per month, over a 20-month period, and structured observations in 324 intervention and 108 control households, approximately 15 months after interventions commenced.ResultsIn the sanitation arms, observed adult use of a hygienic latrine was high (94–97% of events) while child sanitation practices were moderate (37–54%). In the handwashing arms, handwashing with soap was more common after toilet use (67–74%) than nonintervention arms (18–40%), and after cleaning a child’s anus (61–72%), but was still low before food handling. In the water intervention arms, more than 65% of mothers and index children were observed drinking chlorine-treated water from a safe container. Reported LNS feeding was > 80% in nutrition arms. There was little difference in uptake between single and combined intervention arms.ConclusionsRigorous implementation of interventions deployed at large scale in the context of an efficacy trial achieved high levels of technology and behavioral uptake in individual and combined WASH and nutrition intervention households. Further work should assess how to achieve similar uptake levels under programmatic conditions.Trial registrationWASH Benefits Bangladesh: ClinicalTrials.gov, identifier: NCT01590095. Registered on April 30, 2012.

Effect of deformation-induced phase transformation on AISI 316 stainless steel corrosion resistance

Abstract Austenitic stainless steels are the mostly used stainless steels in sheet metal forming and are subject of a large variety of applications including plate heat exchangers. During cold plastic deformation of heat exchanger plates, the martensitic transformation can take place. Martensite formation can be considered as a function of temperature, stress or strain. This paper is focused on the influence of martensite transformation on the corrosion resistance of AISI 316 austenitic stainless steel. Corrosion analysis showed that martensitic transformation which occurs due to cold forming process and under cyclic loading conditions had a substantially influence on the corrosion resistance of plate heat exchangers made by AISI 316 austenitic stainless steel. The role of molybdenum in the passive film formation has been considered and it was attributed to the ability of Mo to form insoluble chloride complexes at the base of pits. However, the protective film formed as a result of passivation or repassivation process was continuously broken due to continuously martensitic transformation as a result of cyclic working conditions of plate heat exchanger. The martensite volume expansion continuously broke the passive film and consequently new unprotected surfaces were exposed to chlorine-treated water, which was used as thermal agent for the plate heat exchanger. During working period, the heat exchanger plates are subjected to fluctuating stresses and strains that may result in cracks or fracture after a sufficient number of fluctuations. Fatigue fractures are caused by simultaneous action of cyclic stress, tensile stress and plastic strain. The martensite transformation as a result of applied cold plastic deformation process, changes not only the steel structure, but also its physical and chemical properties. The magnetic properties of stainless steel are very dependent on the elements added into the alloy. Having different corrosion potentials, it is easy for them to become the anode and the cathode, respectively, of a corrosion battery.

Analysis of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and its brominated analogues in chlorine-treated water by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS)

A simple, selective and sensitive method for the analysis of the strong mutagen 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and its brominated analogues (BMXs) in chlorine-treated water has been developed. The method is based on gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS), previous liquid–liquid extraction (LLE) of a smaller sample volume compared to other methods and on-line derivatization with a silylation reactive. GC-QqQ-MS/MS has been raised as an alternative easier to perform than gas chromatography coupled to high resolution mass spectrometry (GC–HRMS) for the analysis of MX and BMXs, and it allows to achieve low LODs (0.3ng/L for MX and 0.4–0.9ng/L for BMXs). This technique had not been previously described for the analysis of MX and BMXs.Quality parameters were calculated and real samples related to 3 drinking water treatment plants (DWTPs), tap water and both untreated and chlorinated groundwater were analyzed. Concentrations of 0.3–6.6ng/L for MX and 1.0–7.3ng/L for BMXs were detected. Results were discussed according to five of the main factors affecting MX and BMXs formation in chlorine-treated water (organic precursors, influence of bromide ions, evolution of MX and BMXs in the drinking water distribution system, groundwater chlorination and infiltration of water coming from chlorination processes in groundwater).

A Case of Healthcare Associated Pneumonia Caused by Chryseobacterium indologenes in an Immunocompetent Patient.

Chryseobacterium indologenes is nonmotile, oxidase, and indole positive Gram-negative aerobic bacilli which is widely found in plants, soil, foodstuffs, and water. It can colonize hospital environment due to ability to survive in chlorine-treated water supplies. Chryseobacteria can also colonize patients via contaminated medical devices such as respirators, intubation tubes, humidifiers, intravascular catheters, and prosthetic valves. Immune suppression, comorbidities, use of broad-spectrum antibiotics, and extreme age are other important risk factors for Chryseobacterium infections. We report a case of an 82-year-old male admitted to our hospital with the complaint of altered mental status with history of trauma, and recent orthopedic and neurosurgery operations. He was transferred to neurosurgery intensive care unit due to respiratory failure. Urine culture yielded extended spectrum beta lactamase (ESBL) (+). E. coli and C. indologenes were isolated from transtracheal aspirate. He was treated with ertapenem, and levofloxacin and discharged with full recovery.

In vivo genotoxicity of treated water containing the cylindrospermopsin-producer Cylindrospermopsis raciborskii.

Cylindrospermopsin (CYN) is an alkaloid commonly produced by some cyanobacteria that has been implicated in outbreaks of human illness. The aim of this study was to investigate the genotoxicity of Cylindrospermopsis raciborskii cellular content (including CYN) and its byproducts resulting from chlorination during water treatment. DNA damage in blood and liver cells was analysed by the comet assay and micronucleus test (MN). Mice were injected intraperitoneally with the following treatments: (a) physiological saline, (b) treated water, (c) treated water plus C. raciborskii extract (CYN producer strain, CYPO-011 K), (d) C. raciborskii extract (CYN producer strain, CYPO-011 K), (e) C. raciborskii extract (CYN non producer strain), and (f) treated water plus C. raciborskii extract (CYN non producer strain) extract. After 48 h, samples were taken to perform tests (blood and liver cells to the comet assay and bone marrow to MN test). The CYPO-011 K had a genotoxic and mutagenic effects on liver and bone marrow cells. The group that received chlorine-treated water plus CYPO-011 K also exhibited genotoxic effects in the liver, as well as in the blood, and a mutagenic effect in blood marrow cells. The results emphasise the need of improving CYN monitoring in waters bodies in order to reduce the risk of human exposure.

Waterborne cholera outbreak following Cyclone Aila in Sundarban area of West Bengal, India, 2009

Following Cyclone Aila, a block of Sundarban area, West Bengal, India, reported an increased number of diarrhoea cases at the end of May 2009. This study was performed to identify the agent and source of the outbreak as well as to propose control measures. The outbreak is described by time, place and person. A matched case–control study was conducted and rectal swabs and water specimens were collected. In total, 1076 probable case patients and 14 deaths (attack rate 44/10 000) were identified. Vibrio cholerae El Tor Ogawa was isolated from two of five probable case patients’ stool specimens. The outbreak started in the fourth week of May, with two peaks in the second and fourth weeks of June, and lasted until August 2009. Compared with controls, cases were more likely to drink non-chlorinated piped water [matched odds ratio (MOR) = 16, 95% CI 4.9–51; population attributable fraction 58%) and were less likely to drink chlorine-treated water (MOR = 0.06, 95% CI 0.02–0.18). Villagers broke the water pipelines near their houses for easy access to water. Piped water specimens and stored drinking water were positive for faecal contamination. Contaminated drinking water was the probable source of the cholera outbreak. Repairing the pipelines, chlorination at household level and educating villagers regarding the danger of breached water pipe connections controlled the outbreak.

Formation Mechanism and Chemical Safety of Nonintentional Chemical Substances Present in Chlorinated Drinking Water and Wastewater

This paper reviews the formation mechanism and chemical safety of nonintentional chemical substances (NICS) present in chlorine-treated water containing organic contaminants. Undesirable compounds, i.e., NICS, may be formed under certain conditions when chlorine reacts with organic matter. The rate and extent of chlorine consumption with organics are strongly dependent on their chemical structures, particularly whether double bonds or sulfur and nitrogen atoms occur in the molecules. Organothiophosphorus pesticides (P=S type) are easily oxidized to their phosphorus compounds (P=O type) in chlorinated water containing HOCl as little as 0.5 mg/l, resulting in an increase in cholinesterase-inhibitory activity. Chlorination of phenols in water also produces a series of highly chlorinated compounds, including chlorophenols, chloroquinones, chlorinated carboxylic acids, and polychlorinated phenoxyphenols (PCPPs). In some of these chloroquinones, 2,6-dichloroalkylsemiquinones exhibit a strong mutagenic response as do positive controls used in the Ames test. 2-phenoxyphenols in these PCPPs are particularly interesting, as they are present in the chlorine-treated phenol solution and they are also precursors (predioxins) of the highly toxic chlorinated dioxins. Polynuclear aromatic hydrocarbons (PAHs) were found to undergo chemical changes due to hypochlorite reactions to give chloro-substituted PAHs, oxygenated (quinones) and hydroxylated (phenols) compounds, but they exhibit a lower mutagenic response. In addition, field work was performed in river water and drinking water to obtain information on chemical distribution and their safety, and the results are compared with those obtained in the model chlorination experiments.

Drinking Water and Potential Threats to Human Health in Nunavik: Adaptation Strategies under Climate Change Conditions

In Nunavik, chlorine-treated water is delivered daily, by tank truck, to the houses, where it is stored in tanks. A large part of the Inuit population continues to depend on an untreated water supply, however. This traditional activity poses certain risks in a region with an abundant presence of migratory animals. Nunavik has also experienced significant climate warming since the beginning of the last decade. The main goal of this study, which took place in 2003 and 2004, was to evaluate drinking habits that may place Nunavik residents at an increased risk of gastroenteric diseases in the context of climate change. During the Amundsen cruise in fall 2004, we observed that raw water from the collection sites most frequently visited (brooks, lakes, rivers) was of good quality in most of the villages. Regular monitoring of these sites is necessary, however, and the public should be warned when the sites become contaminated. Of particular concern was the water from the individual storage containers, which was much more contaminated than the water at the collection sites. To develop or improve the climate change adaptation strategies in this area, we propose 1) establishing an appropriate environmental monitoring system, 2) improving wastewater disposal and municipal water systems, 3) involving nursing staff in microbiological testing of the water at community sites, 4) raising public awareness of the risks related to raw water consumption, and 5) gathering strategic health information during the periods of the year when cases of gastroenteric diseases are most frequent, in order to establish whether there is a link between these disorders and water quality.

Characterization of Polyhalogenated Phenoxyphenols (Br/Cl-Predioxins) Formed during Aqueous Chlorination of Phenol in the Presence of Bromide Ion

Aqueous phenol solution with bromide ion was treated with sodium hypochlorite at 20°C under various experimental conditions. Changes in the composition and the concentration of the halogenated products in water were determined by gas chromatographic (GC) and GC-mass spectrometric (GC/MS) analyses of diethyl ether extracts. Phenol was shown to produce a variety of halogenated compounds, including polybrominated/chlorinated phenoxyphenols (PXPPs, Br/Cl-predioxins), having three to five halogen atoms, as byproducts in the chlorine-treated water. Production of these PXPPs is dependent on the number of equivalent chlorine atoms per mole of compound and on the reaction pH. These findings suggest that the chlorinetreatment of water contaminated with phenol and bromide ion leads to the formation of Br/Cl-predioxins, which are precursors of the highly toxic halogenated dibenzo-p-dioxins.

Characterization of Polyhalogenated 4-Methylphenol Dimers (Br/Cl-Predioxins) Formed during Aqueous Chlorination of 4-Methylphenol Solution in the Presence of Bromide Ion

Aqueous 4-methylphenol solution with bromide ion was treated with hypochlorite at 20°C under various experimental conditions. Changes in the composition of the halogenated products in water were determined by gas chromatographic (GC) and GC-mass spectrometric (GC/MS) analyses of diethyl ether extracts. 4-Methylphenol was shown to produce a variety of halogenated compounds, including polybrominated/chlorinated phenoxyphenols (Br/Cl-predioxins), having one to four halogen atoms, as by-products in the chlorine-treated water. Production of these predioxins is dependent on the number of equivalent of chlorine atoms per mole of compound and on the reaction pH. These results suggest that the chlorine treatment of water contaminated with 4-methylphenol and bromide ion leads to the formation of Br/Cl-predioxins, which are precursor of the highly toxic halogenated dibenzo-p-dioxins.

Multi-route trihalomethane exposure in households using municipal tap water treated with chlorine or ozone–chlorine

In Korea, data for multi-route trihalomethane (THM) exposure in households using municipal tap water treated with ozone–chlorine or chlorine are unavailable or very limited. Accordingly, the present study was designed to obtain those data by measurements of the THM concentrations in the tap water and indoor and outdoor air in the two types of households, along with an estimation of THM exposure from water ingestion, showering, and the inhalation of indoor air. Chloroform was the most abundant THM in all three media, yet no bromoform was detected in any sample. Similar to previous findings, the winter chloroform concentration in tap water treated with chlorine (22.1 μg/l, median) was significantly higher than that in the tap water treated with ozone–chlorine (16.8 μg/l, median). However, the summer water chloroform concentrations and summer and winter water concentrations of the other two THMs (bromodichloromethane and dibromochloromethane) exhibited no significant difference between the chlorine and ozone–chlorine-treated water. It was suggested that the effects of the water parameters including biochemical oxygen demand of raw water entering water treatment plants should be considered when evaluating the advantage of ozone–chlorine disinfection for THM formation over chlorine disinfection. The indoor air THM concentration trend was also consistent with the water concentration trend. The indoor to outdoor air concentration ratios were comparable with previous studies. The THM exposure estimates from water ingestion, showering, and the inhalation of apartment indoor air when not in the shower suggested that, for residents living in the surveyed households, their exposure to THMs in the home was mostly associated with their household water uses. The THM exposure estimates from tap water ingestion were similar to those from showering.

An Approach to the Rehabilitation of <I>Kogia</I> spp.

Abstract Pygmy (Kogia breviceps) and dwarf (K. sima) sperm whales are rarely seen in the wild, but often seem to live-strand, particularly in cow-calf pairs. The rehabilitation of live-stranded individuals of both species has proven to be exceedingly diffi-cult. The few released animals might not have been completely healthy, an alternative chosen due to their poor survival in captivity. The rehabilitation challenges for Kogia are numerous because limited knowledge exists regarding even the basic biology of both species. This report provides information derived from the rehabilitation of 13 live-stranded K. breviceps and K. sima (including five calves) over the last decade at the Dolphin and Whale Hospital at Mote Marine Laboratory and Aquarium in Sarasota, Florida. One K. breviceps calf survived for almost 21 months in captivity and one K. sima survived for over 15 months, both apparent world-wide records. From these cases we learned that it is critical to provide supplemental fluids in addition to solid food to maintain continuous activity of the intestinal tract, especially if maintained in chlorine-treated water, and that digestibility of squid species typically fed to captive marine mammals was poor. Both species appear to be susceptible to adverse reactions to a number of the drugs commonly used during rehabilitation. In addition, an artificial calf formula was developed to provide adequate nutri-tion for young calves. Finally, gastric and intesti-nal stasis appears to lead to death in many of these whales in captivity.Key Words: Rehabilitation, pygmy sperm whale, dwarf sperm whale, calf formula, orphan calves, survival, captivity, Kogia breviceps, Kogia sima

RAPID COMMUNICATION: WATER DISINFECTION BY-PRODUCTS ENHANCED APOPTOTIC ACTIVITY IN HUMAN LYMPHOCYTES

The apoptosis-inducing activity of concentrated sediments of disinfected and non-disinfected water samples from the waterworks in Budapest, Hungary, was investigated using cultures of human peripherial blood lymphocytes. Chlorine-treated water and untreated (raw) water sediments were concentrated with the use of Amberlite XAD-2 resin columns. The concentrates were dissolved in dimethyl sulfoxide and added to cultures of human peripherial blood lymphocytes. Apoptotic index was determined in lymphocytes after treatment with the raw or disinfected concentrates by flow cytomerty. Disinfected water concentrates of 100μl/ml increased the apoptotic ratio of lymphocyte culture. The same amount of raw water concentrate also enhanced apoptosis. Both raw water and disinfected water contain substances that induced a significant rate of apoptosis in lymphocyte cultures.

Genotoxic activity of chlorohydroxyfuranones in the microscale micronucleus test on mouse lymphoma cells and the unscheduled DNA synthesis assay in rat hepatocytes

Chlorohydroxyfuranones (CHFs) are mutagenic disinfection by-products found in chlorine-treated drinking water. In the current study, the genotoxicity of four CHFs, 3,4-dichloro-5-hydroxy-2(5H)-furanone (MCA), 3-chloro-4-methyl-5-hydroxy-2(5H)-furanone (MCF), 3-chloro-4-(chloromethyl)-5-hydroxy-2(5H)-furanone (CMCF) and 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), was determined. Two in vitro assays, the microscale micronucleus assay on L5178Y mouse lymphoma cells and the unscheduled DNA synthesis assay on a hepatocyte primary culture from Fisher F344 rats, were carried out. All four CHFs demonstrated genotoxic effects in both assays. In the two systems used, CMCF was the most genotoxic compound, followed by MCA, MX and MCF, respectively. This work was the first study of the DNA damaging properties of all four CHFs in two in vitro genotoxicity tests. These new data expand the range of genetic damages induced by this group of compounds.