Chlorhexidine Digluconate Solution Research Articles

Bacterial contamination of air and surfaces during dental procedures-An experimental pilot study using Staphylococcus aureus.

The oral cavity contains numerous microorganisms, including antimicrobial-resistant bacteria. These microorganisms can be transmitted via respiratory particles from patients to healthcare providers and vice versa during dental care. We evaluated the spread of Staphylococcus aureus during standardized dental procedures using different scaling devices and rinsing solutions. During systematic therapy for dental biofilm removal (guided biofilm therapy), using an airflow or ultrasound device to a model simulation head. Staphylococcus aureus suspension was injected into the mouth of the model to mimic saliva. Different suction devices (conventional saliva ejector or a prototype) and rising solutions (water or chlorhexidine) were used. To assess contamination with S. aureus, an air-sampling device was placed near the oral cavity and samples of surface areas were collected. S. aureus was only detected by air sampling when the conventional saliva ejector with airflow was used. No growth was observed during treatments with the ultrasonic piezo instrument or the prototype suction device. Notably, a rinsing solution of chlorhexidine digluconate decreased the bacterial load compared to water. Surface contamination was rarely detected (1 of 120 samples). Although our findings indicate potential airborne bacterial transmission during routine prophylactic procedures, specific treatment options during biofilm removal appear to reduce air contamination. These options include ultrasonic piezo devices or the prototype suction device. The use of chlorhexidine reduced the CFU counts of S. aureus detected by air sampling. Surface contamination during dental procedures was a rare occurrence.

Study of the Antimicrobial Activity of Staphylococcal Bacteriophage on a Model of an Infected Skin Wound

The purpose of this study was to investigate the antimicrobial activity of staphylococcal bacteriophage on a model of a skin wound infected with Staphylococcus aureus. The study of antimicrobial activity was carried out in vivo by experimental modeling of a skin wound infected with S.aureus in the interscapular region in nonlinear male rats. All manipulations with animals were performed in accordance with the requirements of regulatory documentation overseeing the management of laboratory animals and work with them. The results obtained indicate the pronounced antimicrobial activity of staphylococcal bacteriophage in conditions of wound infection when applied externally, which is confirmed by a decrease in the degree of bacterial contamination of wound discharge, as well as a decrease in inflammatory phenomena, both at the local level in the form of a decrease in hyperemia, and generalized in the form of a decrease in C-reactive protein levels, total leukocyte count, as well as a decrease in the leukocyte inflammatory index. The identified antimicrobial effect is comparable to the external use of an antiseptic drug — a solution of chlorhexidine digluconate 0.05%, however, when assessing microbial contamination, it was found that the bacteriophage exhibits more pronounced antibacterial activity. Thus, the staphylococcal bacteriophage showed pronounced antimicrobial activity in a model of a skin wound infected with S.aureus, confirmed by a decrease in the number of colonies of the microorganism in the wound discharge, as well as a decrease in the severity of inflammation.

Эффективность лекарственного препарата «Аргумистин®» при обработке операционного поля и швов после оперативного вмешательства

This paper presents the results of a study of the effectiveness of an antiseptic when treating the surgical field and sutures after surgery. It has been established that the antiseptic agent «Argumistin 0,005 %» has an equal degree of effectiveness compared to traditional methods of seam treatment. Complications when using the antiseptic were not recorded in any case, allergic reactions and signs of inflammation were also absent. Thus, this product can be recommended for use in veterinary clinics when treating sutures after surgical operations. It has been established that the antiseptic «Argumistin 0,005 %», along with a 0,01 % solution of chlorhexidine digluconate, effectively prevents the development of surgical infection when treating the surgical field, is well tolerated by animals, does not cause allergic reactions and the development of inflammation.

Homemade Nucleic Acid Preservation Buffer Proves Effective in Preserving the Equine Faecal Microbiota over Time at Ambient Temperatures.

The equine faecal microbiota is often assessed as a proxy of the microbial community in the distal colon, where the microbiome has been linked to states of health and disease in the horse. However, the microbial community structure may change over time if samples are not adequately preserved. This study stored equine faecal samples from n = 10 horses in four preservation treatments at room temperature for up to 150 h and assessed the resulting impact on microbial diversity and the differential abundance of taxa. Treatments included "COLD" (samples packaged with a cool pack), "CLX" (2% chlorhexidine digluconate solution), "NAP" (nucleic acid preservation buffer), and "FTA" (Whatman FTA™ cards). The samples were assessed using 16S rRNA gene sequencing after storage for 0, 24, 72, and 150 h at room temperature under the different treatments. The results showed effective preservation of diversity and community structure with NAP buffer but lower diversity (p = 0.001) and the under-representation of Fibrobacterota in the FTA card samples. The NAP treatment inhibited the overgrowth of bloom taxa that occurred by 72 h at room temperature. The COLD, CLX, and NAP treatments were effective in preserving the faecal microbiota for up to 24 h at room temperature, and the CLX and NAP treatments improved the yield of Patescibacteria and Fibrobacterota in some cases. The cold and CLX treatments were ineffective in preventing community shifts that occurred by 72 h at room temperature. These findings demonstrate the suitability of the COLD, NAP, and CLX treatments for the room temperature storage of equine faeces for up to 24 h and of NAP buffer for up to 150 h prior to processing.

HERPETIC GINGIVOSTOMATITIS ASSOCIATED WITH OPPORTUNIST SECONDARY BACTERIAL INFECTION IN ODONTOPEDRIATRIC PATIENT

Herpetic gingivostomatitis is a primary infection caused by the manifestation of the herpes simplex virus (HSV). The present study outlines the case of a White 1.6-year-old male patient complaining of pain, dysphagia, bulging in the right submandibular region, and the presence of multiple ulcerations in the mouth. On oroscopic examination, besides the ulcerations, the presence of exudate, which indicates the existence of an opportunistic secondary bacterial infection, was also noted. Palpable submandibular, submental, and digastric lymph nodes were observed. Bacterial culture was requested. The treatment was carried out with antibiotic therapy with a prednisone ointment with lidocaine. Concomitantly, 7 sessions of photodynamic therapy were completed along with a daily mechanical cleaning with chlorhexidine digluconate solution. The procedures adopted in the case were based on 2 infections of different origins. The result was satisfactory because there was a complete regression of the lesions.

Chlorhexidine (di)gluconate locking device for central line infection prevention in intensive care unit patients: a multi-unit, pilot randomized controlled trial

Intensive care unit (ICU) patients are at risk for central line-associated bloodstream infection (CLABSI) with significant attributable mortality and increased hospital length of stay, readmissions, and costs. Chlorhexidine (di)gluconate (CHG) is used as a disinfectant for central line insertion; however, the feasibility and efficacy of using CHG as a locking solution is unknown. Patients with a central venous access device (CVAD) in situ were randomized to standard care or a CHG lock solution (CHGLS) within 72 hours of ICU admission. The CHG solution was instilled in the lumen of venous catheters not actively infusing. CVAD blood cultures were taken at baseline and every 48 hours. The primary outcome was feasibility including recruitment rate, consent rate, protocol adherence, and staff uptake. Secondary outcomes included CVAD colonization, bacteraemia, and clinical endpoints. Of 3,848 patients screened, 122 were eligible for the study and consent was obtained from 82.0% of the patients or substitute decision makers approached. Fifty participants were allocated to each group. Tracking logs indicated that the CHGLS was used per protocol 408 times. Most nurses felt comfortable using the CHGLS. The proportion of central line colonization was significantly higher in the standard care group with 40 (29%) versus 26 (18.7%) in the CHGLS group (P=0.009). Using a device that delivers CHG into CVADs was feasible in the ICU. Findings from this trial will inform a full-scale randomized controlled trial and provide preliminary data on the effectiveness of CHGLS. ClinicalTrials.gov Identifier NCT03309137, registered on October 13, 2017.

Treatment of infected wounds of the abdominal wall after mesh hernia repair

Background. An infected wound of the abdominal wall after mesh hernia repair is related to mesh infection, because mesh is situated at the bottom of the wound and granulation tissue doesn’t cover it. Infected mesh wounds have traditionally been treated by surgically removing the mesh which causes prolonged hospitalizations and hernia recurrence. Objective of the study is to increase the efficiency of treatment of wounds infection after mesh heria repair.
 Маterials and methods. We analyzed the results of wound infection treatment in 72 patients. 37 patients (group I) were treated according to the developed algorithm (ultrasound cavitation, VAC-therapy and decamethoxine solution), 35 (group II) were treated according to the traditional one (ultrasound cavitation, VAC-therapy and chlorhexidine digluconate solution).
 Results. The number of microbial colonies (CFU) in the wound decreased to lg (2.33 ± 0.51) CFU/ml and lg (1.35 ± 0.49) CFU/ml in group I against lg (4.01 ± 0) CFU/ml and lg (2.93 ± 0.56) CFU/ml in group II. (p < 0.01) The results of morphological examination of the wound edges confirmed more efficient growth of connective tissue in patients of group I (mesh was completely covered with granulation tissue) which improved wound healing. While in group II, mesh explantation was performed in 5 (14.3 %) patients (granulation tissue hasn’t covered mesh completely). The duration of wound healing in patients of group I was 16 ± 1.05 days, compared with 21 ± 1.2 days in group II (p < 0.01). We achieved better results through the use of the developed algorithm in group I, in particular the combination of ultrasound cavitation, VAC therapy and decametoxine solution, which has not only antimicrobial action, but also promotes connective tissue repair in the wound.
 Conclusion. Developed algorithm for wounds infection treatment after alloplasty, which includes ultrasound cavitation, VAC-therapy and decamethoxine solution, improves treatment outcomes, reduced wound healing time to 16 ± 1.05 days in patients of the main group against 21 ± 1.2 in comparison group, and excludes the possibility of mesh explantation in the main group against 14.3 % in the comparison group.

Influence of chlorhexidine, propolis, pulpal pressure simulation, and aging on dentin bond strength.

The present study evaluated the bond strength (μTBS) of dentin treated with chlorhexidine and propolis subjected to simulated pulpal pressure (SPP) and thermocycle aging. One hundred and twenty healthy human molars were sectioned to obtain 2 mm of dentin thickness and were divided into two groups (n=60): SPP (15 cm H2 O) and no SPP (Control group). Dentin surfaces were conditioned with 37% phosphoric acid for 15 s and were divided according to the dentin treatment (n=20): Control; Chlorhexidine gluconate (0.2% for 30 s) and Propolis (aqueous propolis extract for 30 s). Half of the specimens were submitted to 15,000 thermocycle aging (5 ± 2°C and 55 ± 2°C). The samples were sectioned into beams and submitted to μTBS. Data were analyzed by three-way ANOVA (SPP × Dentin treatment × Thermocycle aging) and the Tukey's tests (p < .001). With regard to the SPP, ANOVA revealed that the Control group (32.98 MPa) had significantly higher values of μTBS when compared to the SPP (29.19 MPa). With regard to Thermocycle aging, no aging (34.05 MPa) had significantly higher values of μTBS when compared to the aging (28.12 MPa). With regard to the dentin treatment, Propolis and Chlorhexidine did not statistically influence the results (p > .05). The SPP and thermocycle aging negatively influenced the bond strength between the dentin and resin; the 0.2% chlorhexidine digluconate and aqueous propolis extract solutions did not interfere in the bond strength between the resin and dentin. The use of chlorhexidine and propolis as a dental treatment may not influence the dentin bond strength, but SPP and thermocycle aging may damage the longitudinal dentin bond strength. RESEARCH HIGHLIGHTS: Chlorhexidine and propolis as a dental treatment may not influence the dentin bond strength. Simulated pulpal pressure and thermocycle aging may damage the longitudinal dentin bond strength.

The behaviour of aluminium ions in artificial saliva and the impact of the chlorhexidine digluconate on its removal – A diffusion model

Diffusion coefficients for aluminum chloride in artificial saliva or chlorhexidine digluconate solutions (pure and commercially, frequently used as a mouthwash), at tracer concentrations (D0T) at 298.15 K and at different pH, as well as ternary diffusion for aqueous AlCl3 and chlorhexidine digluconate solutions, were measured by using the Taylor dispersion technique.Notable coupled diffusion of AlCl3 and chlorhexidine digluconate is observed by obtaining of the negative values of the cross-diffusion coefficients, D21. These results show the occurrence of complex structures formed between Al3+ and gluconate anions. Support for these facts are given by molecular dynamics simulations (MDS).

Efficacy of a Commercially Available Herbal Formulation for the Disinfection of Elastomeric Heavy Body Impression Materials

Introduction: Cross infection refers to the transfer of harmful microorganisms from one person to another. Dental impressions are considered semi-critical as it directly comes in contact with patient’s saliva and oral mucosa. Improper handling of these dental impressions can potentially have the risk of transmitting infections especially to the dentist. The role of herbal preparation in disinfecting the dental impressions is sparse in the literature. Aim: To evaluate and compare the disinfection ability of a commercially available herbal formulation (HiOra®) with chlorhexidine digluconate solution (0.2%) and 1% sodium hypochlorite on dental impressions made using condensation silicone. Materials and Methods: A cross-sectional study was conducted in Outpatient Department of Periodontology and Implantology at Sri Ramachandra Institute of Higher Education and Research, Chennai, Tamil Nadu, India, between June 2019 and November 2019. Sixty maxillary and mandibular dental impressions using condensation silicone material were obtained from 30 systemically healthy volunteers. The impressions were divided into three groups and were subjected to three antimicrobial agents for 10 minutes: 1% Sodium Hypochlorite (group I); 0.2% chlorhexidine digluconate (group II); HiOra® (group III), respectively. Following this, microbial culturing was performed to detect the presence of Streptococcal and Staphylococcus species in samples obtained both prior and after impression disinfection. One-way Analysis of Variance (ANOVA) was performed to assess the significance of the difference in microbial reduction between the groups. Results: Antimicrobial efficacy was similar against both Streptococcus spp., and Staphylococcus spp., for the three disinfectants tested. No statistically significant difference in the microbial colony count reduction between the three disinfectants tested was observed (Streptococcus spp, p-value=0.064, Staphylococcus spp., p-value=0.337). Conclusion: The herbal mouthwash was found to have an equivalent efficacy as chlorhexidine, sodium hypochlorite for disinfection of impressions made from condensation silicone.

Chlorhexidine (di)gluconate locking device for central line infection prevention in intensive care unit patients: A multi-unit, pilot randomized controlled trial

Chlorhexidine (di)gluconate locking device for central line infection prevention in intensive care unit patients: A multi-unit, pilot randomized controlled trial

Manejo odontológico na Unidade de Terapia Intensiva no tratamento da necrólise epidérmica tóxica associada a fenitoína: relato de caso

ABSTRACT Stevens-Johnson syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) are adverse reactions, usually medicine-related, in which extensive and painful mucocutaneous lesions are formed. Oral lesions are characterized by the presence of erosions and ulcers. The treatment usually requires intensive care in a hospital setting, where the Dentist is essential in the multidisciplinary team, as he will act in the treatment of oral lesions. In this article, a 26-year-old female patient was reported, who presented TEN-compatible mucocutaneous lesions associated with the drug Phenytoin and required admission to a Teaching Hospital. The treatment consisted of the immediate suspension of the Phenytoin use and systemic therapy with corticosteroids and immunosuppressants. Oral lesions were monitored by the hospital dentistry team. The procedures adopted were: oral hygiene with 0.12% non-alcoholic Chlorhexidine Digluconate solution, hydration of the oral tissue with an essential fatty acid, application of frozen chamomile tea, and prescription of topical Triamcinolone Acetonide. Also, low-level laser therapy sessions (red and infrared) were performed. Oral lesions evolved with significant improvement. In this case, the importance of the Dentist in the multi-professional team is demonstrated. Although there is no protocol, the procedures used in the present case can be an alternative to therapies used in SSJ and TEN oral lesions.

Activity of chlorhexidine formulations on oral microorganisms and periodontal ligament fibroblasts

Abstract&#x0D; Given the importance of microorganisms in the pathogenesis of the two most prevalent oral diseases (i.e. caries and periodontitis), antiseptics are widely used. Among the antiseptics chlorhexidine (CHX) is still considered as gold standard. The purpose of this in-vitro-study was to determine the antimicrobial activity of new CHX digluconate containing formulations produced in Switzerland. Two test formulations, with 0.1% or 0.2% CHX (TestCHX0.1, TestCHX0.2) were compared with 0.1% and 0.2% CHX digluconate solutions (CHXph0.1, CHXph0.2) without additives and with a commercially available formulation containing 0.2% CHX digluconate (CHXcom0.2). The minimal inhibitory concentrations (MIC) of the CHX formulations were determined against bacteria associated with caries or periodontal disease. Then the anti-biofilm activities of CHX preparations were tested regarding inhibition of biofilm formation or against an existing biofilm. Further, the cytotoxicity of the CHX preparations against periodontal ligament (PDL) fibroblasts was measured. There were no or only minor differences of the MIC values between the CHX preparations. Except for 0.1% CHXph, all formulations blocked the formation of the biofilms. When analyzing the effect on an established “cariogenic” biofilm, the TestCHX0.2 was most active (- 3.5 log10 colony forming units (cfu); p&lt;0.001 vs. control). The cfu counts of an established periodontal biofilm were most decreased by CHXcom0.2 (-3.9 log10), followed by TestCHX0.2 (-3.34 log10) and CHXph0.2 (-3.0 log10 cfu) (each p&lt;0.001 vs. control). All CHX preparations reduced the viability of the PDL fibroblasts. Taken together, the Swiss made test mouth rinses have an equal or better antibacterial and anti-biofilm activity as the respective CHX solutions and can be recommended. &#x0D; Accepted for publication: December 17, 2020&#x0D; Published online: January 28, 2021&#x0D; Read the complete article as PDF file.

Prospección de extractos de plantas como complementos de la higiene bucal en perros Labrador Retriever

The present study aimed to prospect plant extracts as a therapeutic resource, compared to the oral microbiota of dogs. For this purpose, ethanol extracts from different parts of Anacardium Ocidentalis (cashew tree), Stryphnodendron adstringens (barbatimão), Punica granatum (pomegranate), Uncaria tomentosa (cat's claw), Psidium guajava L. (guava), Momordica charantia L. (melon from São Caetano) and Harpagophytum procumbens (devil's claw), prepared in different concentrations. Microorganisms collected from the oral mucosa of 12 Labrador dogs, maintained under the same management and feeding, were seeded in BHI Agar in a petri dish in which paper disks impregnated with 20µL of each extract were circulated. 0.12% chlorhexidine digluconate solution was used as a positive control. The plates were incubated for 12 hours at 36 ºC. Three swabs were collected for each animal and each experiment was carried out in triplicate. The results of the inhibition halo measurements were subjected to the Kruskall-Wallis analysis of variance, followed by the Dunn test, with a significance level of 5%. Based on the results, pomegranate, guava, and barbatimão extracts were selected, as they were statistically significant at concentrations of 250; 125; 62,6mg/mL which suggests that the three are potential candidates for use in the development of pharmaceutical products for use in oral hygiene of dogs.

Antimicrobial Capacity and Surface Alterations Using Photodynamic Therapy and Light Activated Disinfection on Polymer-Infiltrated Ceramic Material Contaminated with Periodontal Bacteria.

This study determined the antimicrobial efficiency of light-activated disinfection (LAD) and photodynamic therapy (PDT) on polymer-infiltrated ceramic network (PICN) material contaminated with three periodontal bacteria and explored if PDT and LAD cause PICN surface alterations. Sixty PICN discs were contaminated with Tannerella forsythia, Porphyromonas gingivalis, and Treponema denticola and randomly divided into five groups (n = 12 samples/each) according to the treatment groups: Group PDT—PDT (630 ± 10 nm diode laser) with methylene blue; Group DL—808 nm diode laser in contact mode without photosensitizer; Group MB–methylene blue without light application; Group CHX—0.12% chlorhexidine digluconate solution and; Group NT—no treatment. Each disc was then placed in tubes containing phosphate buffered saline (PBS) and vortexed for 30 s to remove the remaining bacteria from the discs. A total of 10× serial dilutions were performed followed by plating of 30 μL of suspension on Brucella agar plates. The colony forming units (CFU) were calculated after 72 h. PICN discs with the attached biofilms were used for confocal microscopy investigation for live/dead bacterial viability. A random single sample from each group was selected to study the bacterial adherence and topographical alterations on PICN discs under scanning electron microscope (SEM). The PDT group showed higher reduction for each bacterial species and total counts of bacteria assessed followed by the DL group (p < 0.05). When compared with MB group, the two laser groups were significantly superior (p < 0.05). The MB group did not show significant differences for any bacteria when compared to NT. The bacteria with the CHX group and DL groups appeared dead with few areas of surviving green stained bacteria. The PDT group showed the highest dead cell count (p < 0.05). PDT and DL groups indicate no significant changes on the surface compared to the sterile PICN discs on visual assessment. Photodynamic therapy produced superior periodontal bacteria reduction over the surface of PICN surface. PDT group showed higher reduction for each bacterial species and total counts of bacteria assessed followed by the DL group. Both PDT and DL treatment strategies are effective without producing surface alterations on PICN.

Chlorhexidine hexametaphosphate as a coating for elastomeric ligatures with sustained antimicrobial properties: A laboratory study

White spot lesions are a common side effect of orthodontic treatment. This laboratory study aimed to explore the suitability of chlorhexidine hexametaphosphate (CHX-HMP) as a coating for orthodontic elastomeric ligatures to provide sustained chlorhexidine (CHX) release. Dissolution kinetics of CHX-HMP were firstly explored using spectroscopy and a colorimetric phosphate assay. Elastomeric ligatures were categorized into 3 groups-acetone-conditioned, ethanol-conditioned, and as received-and were then immersed in 5mM CHX-HMP suspension or 5mM chlorhexidine digluconate solution and rinsed. CHX release was measured over 8weeks, and the effects of conditioning and immersion on elastomeric force and extension at rupture and surface topography were investigated. CHX-HMP exhibited a gradual equilibration that had not reached equilibrium within 8weeks, releasing soluble CHX and a mixture of polyphosphate and orthophosphate. CHX digluconate-treated ligatures showed no CHX release, whereas CHX-HMP-treated ligatures showed varying degrees of release. As received, CHX-HMP-treated ligatures showed a modest release of CHX up to 7days. Acetone conditioning did not enhance CHX-HMP uptake or subsequent CHX release and caused a deterioration in mechanical properties. Ethanol conditioning enhanced CHX-HMP uptake (6×) and led to a sustained CHX release over 8weeks without affecting mechanical properties. Within the inherent limitations of this in-vitro study, CHX-HMP led to a sustained release of CHX from orthodontic elastomeric ligatures after ethanol conditioning. Conditioned and coated elastomeric ligatures may ultimately find application in the prevention of white spot lesions in orthodontic patients.

Mucocutaneous Horn in Dog

Background: Cutaneous horn is a circumscribed exophytic lesion composed of dense, compact keratin with hyperplastic epidermis, which is primarily orthokeratotic and may include foci of parakeratosis. The hyperkeratotic protuberance resembles a horn but lack bone. In humans, it is well-documented with a wide range of primary epidermal lesions identified. In dogs, the reports are rare and brief. The diagnosis is based on its appearance and excisional biopsy reveals the triggering lesion. The objective of the present work is to describe the clinical presentation, predictive benignancy finds, treatment and follow-up of a case of cutaneous horns that arose from anal mucocutaneous boundary in a dog.Case: A 6-month-old male Pug presented two tumors in the anus noted in the early months of life. Complete blood count, serum chemistry profile, surgery, histopathological analysis and postoperative outcome were performed. Possible relapses were follow-up until 11 months after surgery. On physical examination, the dog was otherwise healthy. The two horn-like tumors were protruding from the mucocutaneous junction of the external anal sphincter in a sun-protected area. Both were higher than wide in base or, in other words, they had a high height-to-base ratio. During surgery, the masses were excised using an electronic scalpel and sent to histopathological analysis. The post-operative care consisted of cephalexin and meloxicam prescriptions. Also, it was recommended 0.9% NaCl solution wound flushing followed by topical chlorhexidine digluconate solution (1%) whenever dirt or defecation were noticed. The surgical wound healed after two weeks by second intention. Histopathology found well-delimited masses that covers the epidermis and dermis and displaces the cutaneous appendages towards the underlying musculature with an aggregate of lymphoplasmohistiocytic inflammatory infiltrate in the adjacent dermis. These finds and the presence of orthokeratotic hyperkeratosis and epidermis hyperplasia confirmed the clinical diagnosis of cutaneous horns. There were no signs of malignance. After surgery, no relapse occurred.Discussion: To the best of the authors’ knowledge, this is the first confirmed case of cutaneous horns in mucocutaneous junction. Furthermore, the tumors were found in a region with little exposure to sunlight which is unusual. The tumor’s narrow bases and the absence of continuous and dense inflammatory infiltrate shown to be predictive of benignancy as occurs in human beings. During surgical planning, it was decided not to establish wide margins around the masses. The decision considered the morphological signs of benignancy of the tumors described for human beings as no surveys about prevalence of benignity or malignancy associated with cutaneous horns were found in dogs. In addition, a more extensive excision could promote anal sphincter dysfunction. There was no tumors recurrence suggesting that the primary underlying lesions have been healed and confirming that predictive benignancy morphological characteristics applied in human patients can be useful for small animals. Veterinary clinicians and surgeons must be in constant vigil of cutaneous horns uncommon presentations and report them to create a solid database that can be useful for prognosis and surgical planning. The morphological predictive factors can be applied to avoid unnecessary extensive surgical excisions that could lead to functional or cosmetic impairment.

Removal of micro-pollutant using an indigenous photo membrane reactor

Pharmaceutical wastewater treatment using heterogeneous photocatalysis in presence of TiO2 nanoparticles has become a lucrative method because the convectional wastewater and biological treatments usually fail to remove the detrimental effect of different drugs and hormones on the environment. The nanoparticles recovery and reuse is the most challenging task in practice. The main goal of the present study is to recover and reuse of photocatalyst by immobilizing it on the membrane surface. Consequently, two types of immobilization technique have been used here, one is surface immobilization by dipped coating, and other is impregnation of catalyst during fabrication of the membrane. To conduct photocatalysis, an indigenous photo membrane reactor has also been fabricated during the research work. The presence of TiO2 nanoparticles has not only improved the catalytic property of the membrane, but also the contact angle analysis has confirmed the enhanced hydrophilic as well as antifouling property of the surface modified membrane. The fabricated membranes were characterized by scanning electron microscopy (SEM). To analyses the performance of photo membrane reactor as well as TiO2 coated or impregnated membranes, the photocatalytic degradation of aqueous solution of chlorhexidine digluconate (CHD), an antibacterial drug, was performed using the developed system and the removal percentage of CHD obtained from the system was found to be quite appreciable. The results show that the recovery and reuse of the catalyst by the present system is very much significant. Meanwhile, the developed reactor can be operated in cross flow configuration under batch, semi-batch and continuous mode.

Anatomical description and in vitro evaluation of the antibacterial potential of Aristolochia esperanzae kuntze (Aristolochiaceae) extract on oral micro-organisms

Background: Aristolochia esperanzae Kuntze (Aristolochiaceae) is a plant present in the Brazilian flora, with few studies in the dental area, but used in integrative therapies to treat some health problems. Objectives: The initial purpose was to make an anatomical description of A. esperanzae and then test its antibacterial activity and minimum inhibitory concentration (MIC) on specific oral micro-organisms-Streptococcus mutans, Porphyromonas gingivalis and Enterococcus faecalis. Materials and Methods: For the pharmacognostic study, the samples were fixed in formaldehyde, acetic acid and 70' ethyl alcohol and stored in 70' ethanol. For the in vitro study, to obtain the essential oil (OE) and hydrolate, the plant material was mechanically macerated and subjected to hydrodistillation in Clevenger and then frozen until the time of testing. The EO was diluted in dimethylsulfoxide and the tests performed were agar diffusion (disc-diffusion) with isolated bacteria and MIC. A 0.12' chlorhexidine digluconate solution was used as a positive control and 0.9' sodium chloride as a negative. Results: The anatomical study showed the presence of hook-shaped trichomes and stomatal complexes. Regarding the microbiological results, they were negative for the extracts used. Conclusion: The anatomical findings may guide future studies related to the pharmacognostic properties of A. esperanzae and both OE and hydrolate did not have an antibacterial effect against S. mutans, P. gingivalis and E. faecalis.

Application of mathematical models and microfluidics in the analysis of saliva mixing with antiseptic solutions

Background/Aim: Human saliva offers many advantages over blood-based biochemical assays, therefore, becomes the biological fluid of interest. Once antiseptic solutions react with saliva, both fluids undergo significant changes of their biophysical properties, consequently, those changes have an impact on their principal function. Material and Methods: In this study, saliva was collected and mixed with 0,1% chlorhexidine digluconate solution, fluoride mouthwash, zinc-hydroxyapatite solution and CPP-ACP paste. Microfluidic PVC/Green tape chips within the experimental setup were used to simulate solution mixing. The chip had 2 inlets and 1 outlet, and channel was designed in Y shape without any obstacles. The inlet channels were set at a 60° angle. The channel width was 600 µm and the diameter of inlets and outlet was 2 mm. For better visualization, blue food coloring was added to the saliva. The procedure was recorded with digital USB microscope camera and afterwards the percentage of mixing was obtained by MATLAB programming language. Results: Obtained results show incomplete mixing of all the solutions with saliva. The value of mixed liquid, when mixing 0,1% chlorhexidine digluconate solution with saliva was 51,11%. In case of medium concentration fluoride mouthwash, result was 84,37%. Zinc hydroxyapatite solution obtained result of 85,24%, and the fourth tested solution, CPP-ACP paste, 83,89%. Conclusions: Analyzed mouthwashes exhibit specific, non uniform behavior during mixing with saliva. Microfluidic setups could be efficiently used in simulating real clinical conditions in laboratory settings. Image processing mathematical models are applicable, accurate and useful in determination of the interaction of saliva with commonly used antiseptic solutions.