Chlorfenapyr Residues Research Articles

Dissipation pattern and dietary risk assessment of chlorfenapyr and methomyl in corn under Egyptian field conditions.

Corn is the second most widely farmed grain for human consumption. Low corn productivity due to damage caused by pests has led to using pesticides to control pest infestations. However, the uncontrolled application of pesticides on corn harms both environmental and human health. Accordingly, field experiments followed good agricultural practices to investigate the dissipation pattern and terminal residues of chlorfenapyr and methomyl in corn and compare the values with established safety limits. Gas chromatography-tandem mass spectrometer coupled with the quick, easy, cheap, effective, rugged, and safe technique was used to analyze residues of chlorfenapyr and methomyl in corn. The average recoveries varied from 94% to 105%, with relative standard deviations (RSDs) of 8%-13% for chlorfenapyr and from 99% to 111%, with RSDs of 10-16% for methomyl. Chlorfenapyr and methomyl residues degraded in corn following a first-order kinetic model, with an estimated half-life (t1/2) of 3.9 and 2.8days, respectively, and significant degradation (91.4%-98.1.5%, respectively) after 14 days. Although the maximum residue limits of chlorfenapyr and methomyl for corn are yet to be formulated in Egypt, the long-term dietary risk for those pesticides was acceptable, with arisk quotient < 100%, according to the national assessments. These findings are required to guide the correct and safe application of these insecticides in Egypt.

Residue and dietary intake risk assessment of lufenuron and chlorfenapyr and its corresponding metabolite in cabbage under field conditions.

A simple, low-cost, and highly sensitive method using a modified QuECHERS procedure based on a liquid chromatography-tandem mass spectrometer (LC-MS/MS) was established to simultaneously quantify lufenuron and chlorfenapyr and the corresponding metabolite tralopyril in cabbage for the first time. On the basis of this method, terminal residue and dietary risk of lufenuron and chlorfenapyr in cabbage were investigated. The recoveries of lufenuron, chlorfenapyr, and tralopyril ranged from 88 to 110%, with relative standard deviation of less than 12.4%. The field trial results showed that at the pre-harvest interval (PHI) of 21 days, the terminal residues of lufenuron, chlorfenapyr, and tralopyril in the supervised trials were not higher than 0.02 mg/kg, and the highest detected residue levels of lufenuron, chlorfenapyr, and tralopyril were 0.047, 0.055, and <0.02 mg·kg-1 at 14-day pre-harvest respectively, which were lower than the maximum residue limits (MRLs) for cabbage established in China. For the dietary risk assessment, the national estimated daily intakes (NEDIs) as proportion of acceptable daily intakes (ADIs) were 80.4% and 29.9% for chlorfenapyr and lufenuron respectively indicating an acceptable dietary risk to Chinese population.

The fate and potential hazards of chlorfenapyr and one metabolite tralopyril in cabbages: A comprehensive investigation

The potential hazards of chlorfenapyr warrant attention owing to its widespread application on vegetables. A comprehensive investigation of the fate of chlorfenapyr in the ecosystem is imperative. This paper presents a method for detecting chlorfenapyr and tralopyril in cabbages, which exhibits good linearity (determination coefficients > 0.99) and satisfactory recoveries (82.50 %–108.03 %). Chlorfenapyr residues in cabbages demonstrate a positive correlation with its application dose and time. Tralopyril can inhibit the dissipation of chlorfenapyr, as evidenced by the half-lives of 5.67–11.14 d (chlorfenapyr) and 6.91–14.77 d (total chlorfenapyr). The results of terminal residues (<2.0 mg/kg) and dietary risk assessment (<100 %) suggest preharvest intervals of 14 d (greenhouse) and 10 d (open-field). Additionally, the uptake of chlorfenapyr in cabbages is limited (translocation factor < 1), while the downward translocation predominantly occurs through phloem transport. The findings provide valuable insights for understanding the fate and potential risks of chlorfenapyr in cabbages.

Insecticidal and residual effect of chlorfenapyr on different surfaces for the control of Sitophilusoryzae

The immediate and residual insecticidal effects of chlorfenapyr applied on concrete and metal surfaces were evaluated against Sitophilus oryzae adults in laboratory bioassays. The dose used was 0.016 mg active ingredient (a.i.)/cm2 which refers to the highest label dose in case of severe infestation. Treated surfaces were either kept in continuous darkness or exposed to light. A series of subsequent bioassays were conducted in the treated surfaces, starting from the day of application and continued for three consecutive months, with new insects each time. For each bioassay, mortality levels were recorded after exposure for 3, 7 and 14 days. At the same time, the dissipation of chlorfenapyr residues on the treated surfaces was estimated at different time intervals (0, 3, 7, 15, 30, 60, 90 and 120 days) after application using a high-performance liquid chromatography system with a UV-detector (HPLC-UV). According to the results, mortality rates of S. oryzae adults after direct exposure to chlorfenapyr-treated concrete surfaces were high, reaching 100% at the end of the first bioassay. However, we observed a gradual reduction in mortality with the increase of the storage (post application) interval. On the contrary, mortality on metal surfaces was high and reached in all cases 100% after 7 days of exposure, regardless of the storage interval. Regarding the residues of chlorfenapyr on the surfaces, they remained relatively constant on metal surfaces for the entire 120-day period in both illuminated and non-illuminated surfaces. On concrete, an initial 20–35% reduction was observed over the first 3 days, whereas chlorfenapyr residues remained constant for the rest of the period regardless of the presence of illumination. Our results indicate that chlorfenapyr is effective for the control of S. oryzae, but its residual efficacy is moderated by the time after application, the surface type and partially by the presence of illumination.

Comparative Insecticide Uptake Characteristics of Chlorfenapyr Suggests an Additional Reason Why Bed Bugs (Hemiptera: Cimicidae) are so Difficult to Control.

Insecticides interact with biochemical sites, disrupting homeostasis, leading to moribundity and death. Considering this process of intoxication, investigating insecticide uptake ensures an understanding of exposure profile. This study evaluated insecticide uptake by bed bugs, Cimex lectularius L., as affected by time and formulation, and a comparative uptake by German cockroaches, Blattella germanica (L.). Insects exposed to chlorfenapyr residues from two Phantom insecticide formulations (i.e., Aerosol and Suspended Concentrate (SC)) were solvent extracted and analyzed by GC-ECD. Chlorfenapyr uptake by bed bugs significantly increased with time, but the rate of external chlorfenapyr adsorption between the two formulations was not significantly different. However, initial exposure and uptake to the aerosol-treated versus SC-treated surfaces were significantly greater by 2.7x. Absorption-rates of chlorfenapyr residues inside the insect, associated with internal insect tissues or exoskeleton-bound, were significantly lower compared with external adsorption, with aerosol and SC formulations accumulating at 4.8x and 14.4x less, respectively. An unexpected formulation effect occurred with aerosol-treated papers and aging of residues. A reformulation of the aerosol provided a greater adsorption of chlorfenapyr at 19.1x that of the original formula after 2 hr. External versus internal uptake of chlorfenapyr by cockroaches was inverse to that seen in bed bugs, with greater chlorfenapyr absorption. Obstructing cockroach mouthparts prevented an internal uptake of chlorfenapyr and provided a similar exposure profile to bed bugs. This work demonstrates a need to evaluate insecticide uptake with formulation and insect morphology to maximize dose, especially in such a morphologically and environmentally isolated insect as the bed bug.

Dissipation behaviour, residue analysis, and dietary safety evaluation of chlorfenapyr on various vegetables in China

ABSTRACT Chlorfenapyr has been widely used in recent years to control a variety of pests on fruit and vegetables. Cabbage, leek, asparagus, and chive are four of the most common green foods consumed word wide; their pesticide residue issues have also received more attention. Therefore, studies on the residue analysis, degradation evaluation and dietary risk assessment based on the complete residue definition of chlorfenapyr on these four vegetables were essential and urgently needed. A reliable analytical method was developed and applied to simultaneously determine the content of chlorfenapyr and its metabolite tralopyril residues on the four vegetables. Recoveries were satisfactory (84%–110% for chlorfenapyr; 83%–106% for tralopyril) at a spiked level of 0.01–1 mg/kg, with intraday precision (n = 5) and interday precision (n = 15) ranging from 1.6% to 8.9% and from 2.4% to 9.1%, respectively. The limits of quantification (LOQs) were all 0.01 mg/kg. On the basis of supervised field trials, the degradation half-lives of chlorfenapyr were 1.2–9.8 days. Chlorfenapyr rapidly degraded on asparagus, but persisted much longer on chive. The terminal concentration of chlorfenapyr residues varied from <0.01 to 0.84 mg/kg. Additionally, the risk quotients (RQs) ranged from 4.7% to 13.8%, suggesting that chlorfenapyr had a negligible risk for chronic dietary intake of these crops. This study was thus significant in evaluating the degradation rate and quality safety of chlorfenapyr on various vegetables and promoted the development of maximum residue limits.

Analysis of Degradation Kinetics and Migration Pattern of Chlorfenapyr in Celery (Apium graveliens L.) and Soil Under Greenhouse Conditions at Different Elevations.

The degradation kinetics and migration pattern of chlorfenapyr in celery and soil at Lhasa and Pengzhou were investigated. A simple, rapid analytical method for the quantification of chlorfenapyr in celery and soil was developed using gas chromatography-tandem mass spectrometer. The results indicated that the half-lives of chlorfenapyr in celeries and soils at Lhasa were 6.3 days and 12.8 days. While the half-lives of chlorfenapyr in celeries and soils at Pengzhou were 6.9 days and 20.4 days. The half-lives of chlorfenapyr in celeries and soils at Lhasa were shorter than that at Pengzhou, while the half-lives of chlorfenapyr in soils at Lhasa and Pengzhou were longer than that in celeries at Lhasa and Pengzhou. The final residues of chlorfenapyr in celeries at Lhasa and Pengzhou were 5.074 ± 0.144 mg/kg and 5.981 ± 0.234 mg/kg after 7 days of spraying, respectively. When chlofenapyr was sprayed to soils only, the average root concentration factor of chlorfenapyr were 3.57-4.02, while the average translocation factor of chlorfenapyr in leaves and stems were 0.28-0.38 and 0.20-0.25, respectively. Chlorfenapyr was easy to migrate from soil to the roots of celery, followed by leaves and stems. The limit value of chlorfenapyr in celery has not been specified in China's National MRL standard (GB 2763 in National food safety standard-maximum residue limits for pesticides in food. Standard, Beijing, 2021), this study was useful to draw up the limit values of chlorfenapyr residues in celery at different elevations.

Residue reduction and risk evaluation of chlorfenapyr residue in tea planting, tea processing, and tea brewing

The chlorfenapyr residues in the entire tea chain, i.e., in tea planting, tea processing, and tea brewing, were systematically investigated. The degradation rate constants of chlorfenapyr in the tea plants ranged from 0.2460 to 0.2870 with the half-life of 2.4–3.0 days, and 87.5–89.9% of the chlorfenapyr in tea shoots dissipated in the interval of 7 days. In the processing process of both black tea and green tea, the chlorfenapyr residue decreased by 59.1–67.6% compared with the residue in tea shoots due to high vapor pressure (1.2 × 10−2 mPa 25 °C), and drying was the key step that dissipated the chlorfenapyr. A low leaching efficiency of 2.2–3.4% from tea leaves to tea infusion, resulted in low water solubility (0.14 mg L−1 25 °C), indicated that >90% of the residual chlorfenapyr was eliminated before the intake of tea infusion. On the basis of these results, an extremely large proportion of the chlorfenapyr deposited on tea shoots was degraded during tea planting, tea processing, and tea brewing, and the health risk was reduced primarily in the first and the last step rather than during tea processing. The remaining 0.2% chlorfenapyr sprayed on the tea shoots represents a negligible health risk based on the RQ assessment. The pesticides with high vapor pressure and low water solubility were more recommended in tea garden for pest control.

Fabrication process and characterization of AgNPs/PVA/cellulose as a SERS platform for in-situ detection of residual pesticides in fruit

In this work, AgNPs/PVA/Cellulose was used as a substrate material for surface Raman scattering enhancement. Silver nanoparticles (AgNPs) was synthesized by Lee and Meisel’s method with the average particles size of 15.4 nm. Then, this silver colloid was made a homogenous coating on polyvinyl alcohol and cellulose film and structural characteristics of this material were determined using Scanning Electron Microscopy (SEM). The findings demonstrated that the Raman shifts of the pesticide will be identified by the SERS method at 1660 cm−1, 2234 cm−1 (strong intensity), and at 3077 cm−1, 1033 cm−1, 1457 cm−1 (medium intensity) when using the excited laser with wavelength of 532 nm. Under excited laser, the limit of chlorfenapyr detection is 1 ppm (mg l−1), allowing determination of chlorfenapyr residue in food. Potential applications identified food samples containing chlorfenapyr residue for rapid detection, low cost, non-destructive nature and minimal sample preparation.

Chlorfenapyr Residue in Sweet Persimmon from Farm to Table

The use of the pesticide chlorfenapyr has been increasing over time, with a consequent wider application to crops. However, there is limited information available on the amount and safety of the residues it leaves on crops. The amount of chlorfenapyr residues in sweet persimmon (Diospyros kaki L.) at both the pre- and postharvest stages were investigated in this study by calculating its biological half-life. The half-life at the preharvest stage was 8.8 days, shorter than that found during the storage periods at 4 and 20°C, when the half-lives were 11.0 and 23.9 days, respectively. In addition, peeling and washing after harvesting reduced residue content. The majority of the chlorfenapyr residues in sweet persimmon were found in the peel of the fruit, with the pulp containing less than 25% of the total. Thus, peeling effectively removed chlorfenapyr residues and diminished the residues below the limit of quantification in the pulp. In addition, washing with 1.0% alcohol and 0.2% Tween 20 solutions effectively removed 47.8 and 55.6% of the residues, respectively. Furthermore, a 1.0% alcohol solution showed high reduction efficiency for other hydrophobic pesticides, such as dimethomorph and fluquinconazole, up to 78.0%. Chlorfenapyr residues in sweet persimmon can be effectively reduced via storage or peeling and washing practices or a combination of them.

Lethal and sublethal effects of insecticides on Engytatus varians (Heteroptera: Miridae), a predator of Tuta absoluta (Lepidoptera: Gelechiidae).

The mirid Engytatus varians (Distant) is a promising biological control agent of the tomato borer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), one of the most destructive pests of tomato (Solanum lycopersicum L.). The effects of five insecticides commonly used on tomato crops in Brazil were evaluated on E. varians in laboratory and semifield conditions. Glass Petri dish with residues of chlorfenapyr, thiamethoxam, and abamectin caused ˃90% mortality in both stages of the predator 72 h post-treatment, except imidacloprid that caused 78% of nymphs mortality. Teflubenzuron caused 24 and 66% mortality on adults and nymphs, respectively. The offspring of females derived from treated nymphs with teflubenzuron was significantly lower than the control but not when females were treated as adults. Longevity of males derived from nymphs treated with teflubenzuron was significantly reduced, but no effects were observed on females. When males and females were treated as adults with teflubenzuron there were no effects on their longevity. In the greenhouse-aged tomato plants, the 2 h-old residues of thiamethoxam, chlorfenapyr, and abamectin caused more than 70% of mortality of third instar of E. varians at 72 h post-treatment, 12 day-old residues of all three compounds caused a mortality lower than 30%. These data suggest that teflubenzuron can be associated with releases of E. varians adults, while the use of other evaluated pesticides should be avoided in this situation. Although, the low persistence of these products indicate that their spraying and later releases of E. varians adults on tomato crops are a possible strategy to control T. absoluta.

Chlorfenapyr, a Potent Alternative Insecticide of Phoxim To Control Bradysia odoriphaga (Diptera: Sciaridae).

Bradysia odoriphaga is the major pest affecting Chinese chive production, and in China, it has developed widespread resistance to organophosphorus insecticides. Chlorfenapyr is a promising pyrrole insecticide with a unique mechanism of action that does not confer cross-resistance to neurotoxic insecticides. However, the effect of chlorfenapyr on organophosphate-resistant B. odoriphaga is not well understood. The present study evaluated the potential of chlorfenapyr for the control of phoxim-resistant B. odoriphaga. The results showed that chlorfenapyr had significant insecticidal activity to B. odoriphaga in multiple developmental stages, and there were no significant differences in susceptibility between the field (phoxim-resistant) and laboratory (phoxim-susceptible) populations. The pot experiment and field trials confirmed the results of our laboratory bioassays. In the field trial, chlorfenapyr applied at 3.0, 6.0, or 12.0 kg of active ingredient (a.i.)/ha significantly decreased the number of B. odoriphaga and improved the yield compared to phoxim at 6.0 kg of a.i./ha and the control conditions. Moreover, the final residues of chlorfenapyr on plants were below the maximum residue limits (MRLs) as a result of its non-systemic activity. These results demonstrate that chlorfenapyr has potential as a potent alternative to phoxim for controlling B. odoriphaga.

Reduction of pesticides residues on okra fruits by different processing treatments

For the first time, the effect of washing, water boiling, chemical boiling, steaming and cooking treatments on the reduction of residues of indoxacarb, fenarimol, acetamiprid and chlorfenapyr in okra fruits was investigated. Residues were analyzed using quick, easy, cheap, effective, rugged, and safe (QuEChERS) method combined with liquid chromatography tandem-mass spectrometry (LC–MS/MS). Our results show that some treatments could significantly decrease pesticide residues. The reduction of residues by washing treatment was not correlated to water solubility: Chlorfenapyr (non-systemic pesticide) was decreased by 90 % and acetamiprid (systemic pesticide) was removed by 48 %. Therefore, we suggest that pesticide residues were washed off by removing the loosely attached pesticides on the okra fruit surface. The time of exposure in both boiling and steaming treatments has no significant effect on reduction of pesticides residues. Additionally, cooking after the washing and boiling treatments reduced the pesticides residues. In particular, acetamiprid was reduced after the cooking treatment to 90 %.

Residues and risk assessment of bifenthrin and chlorfenapyr in eggplant and soil under open ecosystem conditions

ABSTRACTDissipation and residue levels of bifenthrin and chlorfenapyr in eggplant and soil under field conditions were investigated using gas chromatography coupled with an electron capture detector (GC-ECD). The mean recoveries of bifenthrin and chlorfenapyr were 85.2–104.9%, with relative standard deviations (RSDs) of 0.5–9.1%. The limit of quantification (LOQ) was 0.01 mg kg−1. Bifenthrin exhibited half-lives of 3.3 to 4.1 days in eggplant and 17.8 to 25.7 days in soil; the half-lives of chlorfenapyr were 3.5 to 3.8 days in eggplant and 21.7 to 27.7 days in soil. During harvest, the terminal residues of bifenthrin and chlorfenapyr were below 0.031 and 0.083 mg kg−1, respectively. Risk assessment for different groups of people in China was evaluated. The risk quotients (RQs) of bifenthrin and chlorfenapyr were ranged from 0.0068 to 0.0148 and from 0.0033 to 0.0072, respectively. These results may provide guidance on reasonable use of pesticides and serve as a basis for establishing maximum residue limits (MRLs) in China.

Residue behavior and risk assessment of mixed formulation of imidacloprid and chlorfenapyr in chieh-qua under field conditions.

A simple and rapid method based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed for the simultaneous determination of imidacloprid and chlorfenapyr residues in chieh-qua. Field trials were designed to investigate the dissipation and terminal residue behavior of the mixed formulation of imidacloprid and chlorfenapyr in chieh-qua in Guangzhou and Nanning areas. Risk assessment was performed by calculating the risk quotient (RQ) values. The developed analytical method exhibited recoveries of 89.9-110.3% with relative standard deviations (RSDs) of 2.8-12.5% at the spiked levels of 0.01, 0.10, and 1.00 mg/kg. The limit of detection (LOD) was 0.003 mg/kg, and the limit of quantification (LOQ) was 0.01 mg/kg for both imidacloprid and chlorfenapyr. It was found that the half-lives of imidacloprid in chieh-qua under field conditions were 3.3 and 3.5 days in Guangzhou and Nanning at a dose of 180 g ai/ha, while the half-lives of chlorfenapyr were 3.3 and 2.6 days, respectively. The terminal residues of imidacloprid and chlorfenapyr were from 0.01 to 0.21 mg/kg and from 0.01 to 0.46 mg/kg, respectively. Results of dietary exposure assessment showed that the RQ values were much lower than 1, indicating that the risk of imidacloprid and chlorfenapyr applied in chieh-qua was negligible to human health under recommended dosage and good agricultural practices. The proposed study would provide guidance for safe and reasonable use of imidacloprid and chlorfenapyr in chieh-qua cultivation in China.

Method validation and dissipation dynamics of chlorfenapyr in squash and okra

QuEChERS method combined with GC–IT-MS was developed and validated for the determination of chlorfenapyr residues in squash and okra matrices. Method accuracy, repeatability, linearity and specificity were investigated. Matrix effect was discussed. Determination coefficients (R2) were 0.9992 and 0.9987 in both matrices. LODs were 2.4 and 2.2μg/kg, while LOQs were 8.2 and 7.3μg/kg. Method accuracy ranged from 92.76% to 106.49%. Method precision RSDs were ⩽12.59%. A field trial to assess chlorfenapyr dissipation behavior was carried out. The developed method was employed in analyzing field samples. Dissipation behavior followed first order kinetics in both crops. Half-life values (t1/2) ranged from 0.2 to 6.58days with determination coefficient (R2) ranged from 0.78 to 0.96. The developed method was utilized for surveying chlorfenapyr residues in squash and okra samples collected from the market. Monitoring results are discussed.

Toxicity of 6 Miticides to the Asian Citrus Psyllid,Diaphorina citri(Hemiptera: Liviidae)

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is an important pest of citrus. Research into strategies to control ACP is ongoing at many facilities, including at the USDA-ARS U.S. Horticultural Research Laboratory (USHRL) in Fort Pierce, Florida. The USHRL maintains colonies of ACP, but their survival is often threatened by mites which render host plants unsuitable for ACP Our objective was to identify miticides/insecticides that could be used to control mite outbreaks with minimal or no adverse affect on ACP We tested the following 6 miticides in greenhouse trials for their toxicity to each life stage of ACP and also investigated sublethal effects on development and oviposition of ACP: bifenazate (Acramite 50 WS), spirodiclofen (Envidor 2 SC), dicofol (Kelthane MF), pyridaben (Nexter), petroleum oil, and chlorfenapyr (Pylon). The miticides differed in their toxicity when applied directly to ACP Bifenazate was the only miticide that was not toxic to any life stage of ACP, whereas pyridaben and chlorfenapyr, which are also labeled as insecticides, were toxic to all life stages of ACP Petroleum oil and dicofol killed adult and nymphal ACP, but were nontoxic to eggs. Spirodiclofen was nontoxic to adults, but reduced nymphal survivorship and killed eggs. The duration of residual activity against adult ACP also was widely variable: dicofol residues were only toxic for up to 10 days, whereas chlorfenapyr residues were still toxic after 36 days. We recommend using dicofol, pyridaben, petroleum oil, and chlorfenapyr to maintain clean plants prior to colonization by ACP and then rotating bifenazate and spirodiclofen, if maintaining adult ACP, or bifenazate, dicofol, and petroleum oil, if maintaining eggs. Bifenazate is the only product safe for maintaining nymphal ACP Our results are useful for research facilities that wish to maintain colonies of ACP and control mites and may be useful for citrus growers and researchers that wish to kill ACP and mites with a single treatment.

Determination of chlorfenapyr in leek grown under greenhouse conditions with GC‐μECD and confirmation by mass spectrometry

A simple analytical method was developed for the determination of chlorfenapyr residues in leeks grown under greenhouse conditions. Residues were extracted by salting out, analyzed by gas chromatography with microelectron-capture detection, and confirmed via gas chromatography-mass spectrometry. The calibration curves were found to be linear with correlation coefficients (r(2) ) in excess of 0.998. The limits of detection and quantification were 0.0015 and 0.005 mg kg(-1) , respectively. For validation purposes, recovery studies were carried out at low and high levels. Yield recovery rates were 87.27-89.64% with a relative standard deviation <6%. A maximum of 0.32 mg kg(-1) of chlorfenapyr residue was detected in leek sample sprayed three times at 7 day intervals until 7 days prior to harvest. The results of this study suggest that chlorfenapyr is acceptable for application in/on leeks under the recommended dosage regimen.

Pesticide dissipation curves in peach, pear and tomato crops in Uruguay*

Dissipation curves of azoxystrobin and of the neonicotinoids acetamiprid and thiacloprid in peach; azinphos-methyl and carbaryl in pear and azoxystrobin, chlorfenapyr and chlorpyrifos in high-tunnel tomato crops were studied in the Southern region of Uruguay. An analytical methodology based on solid phase extraction (SPE) and detection by High Performance Liquid Chromatography with Diode Array Detector (HPLC/DAD) was used for acetamiprid and thiacloprid. Coupled SPE and detection by Gas Chromatography with Mass Selective Detector (GC/MSD) was used for the detection of azinphos-methyl, azoxystrobin, carbaryl, chlorfenapyr and chlorpyrifos residues. Curves were modeled mathematically with Solver program of Microsoft Excel®. The best fit for acetamiprid and thiacloprid in peach was achieved with the exponential model (r2=0.961 and 0.944, respectively). In the case of peach fruits there is not a Maximum Residue Limit (MRL) for acetamiprid in the Codex Alimentarius, while 0.5 mg/kg is the value rated for thiacloprid. The MRLs accepted by the European Union (EU) are 0.1 mg/kg for acetamiprid and 0.3 mg/kg for thiacloprid. According to the curves determined in these experiments, thiacloprid residues 10 to 12 days after application (daa) were below the MRLs established by both sources. In the case of acetamiprid, 25 daa would be required, according to the exponential mathematical model, to get residues levels below the MRL values established by the EU. For azinphos methyl in pear, the residues detected were mathematically fitted to an exponential model (r2=0.999). According to it, residue levels under the MRL established by the EU (0.05 mg/kg) are gotten in our conditions in 20 daa. In plastic tunnel tomato chlorfenapyr residues were not detected from 16 daa, having the dissipation curve an exponential trend. In the same condition, there was not a decay of the azoxystrobin concentration during a 24-day trial, being it around 0.40 ± 0.05 mg/kg.

Degradation Dynamics of Chlorfenapyr Residue in Chili, Cabbage and Soil

Chlorfenapyr is a pyrrole group of insecticide, [4-bromo-2-(4-chlorophenyl)-1-ethoxymethyl-5-trifluoromethyl-1H-pyrrole-3-carbonitrile]used as broad spectrum insecticide/acaricide to control whitefly, thrips, caterpillars, mites, leafminers, aphids, etc., chlorfenapyr 10% SC formulation was applied on chili and cabbage twice @ 75 and 100 g a.i./ha along with untreated control. Chlorfenapyr was dissipated in chili, cabbage and soil following the first-order kinetics (logC/C(0) = -kt). The half lives of chlorfenapyr in chili, cabbage and soil were varying from 2.93 to 2.96 days, 2.98 to 3.62 days and 4.06 to 4.36 days respectively, according to the application rate.