Chlorantraniliprole Residues Research Articles

Dissipation kinetics and decontamination of chlorantraniliprole and thiamethoxam residues in curry leaves

ABSTRACT Herein, using liquid chromatography – tandem mass spectrometry, we validated a modified QuEChERS method to analyse thiamethoxam and chlorantraniliprole residues in curry leaves. These insecticides are part of a pest management module against the major curry leaf insect pests, psyllid and leaf roller. Based on the SANTE guideline, the method exhibited satisfactory recovery and precision. For both insecticides, the R2 value was 0.99. Furthermore, the determined limit of detection and limit of quantification were 0.008 and 0.025 mg/kg, respectively. The field persistence of both insecticides was longer, and below quantification limit was achieved within 30 and 40 days for chlorantraniliprole and 35 and 40 days for thiamethoxam. Residue degradation followed first-order kinetics, with longer half-lives of 5.08 and 5.28 days for chlorantraniliprole and 3.50 and 3.96 days for thiamethoxam at single (x) and double (2×) doses, respectively. For both insecticides, the hazard index (HI), calculated based on the highest residue on the day of insecticide treatment in the field study, was less than 1 at x and 2× doses, indicating an acceptable level of risk. Seasoning or quick frying of curry leaves in oil reduced maximum of thiamethoxam and chlorantraniliprole residues followed by treatment with NaCl (2%)/tamarind water (2%)/lemon water (2%) solutions. Analysis of 85 farm-gate samples revealed detectable residues of thiamethoxam in 25 samples (0.03–0.49 mg kg−1) and chlorantraniliprole in 1 sample (0.18 mg kg−1). The HI for the maximum quantity of insecticide residues detected in monitoring study was <1. Therefore, the research backs the idea of ensuring safe harvested produce, considering the field persistence of chlorantraniliprole and thiamethoxam in curry leaf and simple decontamination methods to satisfy consumer need for clean food commodity, if properly adopted.

Spatiotemporal distribution, ecological risk assessment, and human health implications of currently used pesticide (CUP) residues in the surface water of Feni River, Bangladesh

Spatiotemporal monitoring of pesticide residues in river water is urgently needed due to its negative environmental and human health consequences. The present study is to investigate the occurrence of multiclass pesticide residue in the surface water of the Feni River, Bangladesh, using an optimized salting-out assisted liquid-liquid microextraction (SALLME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The optimized SALLME method was developed and validated following the SANTE/11312/2021 guidelines. A total of 42 water samples were collected and analyzed to understand the spatiotemporal distribution of azoxystrobin (AZ), buprofezin (BUP), carbofuran (CAR), pymetrozine (PYM), dimethoate (DMT), chlorantraniliprole (CLP), and difenoconazole (DFN). At four spike levels (n = 5) of 20, 40, 200, and 400 μg/L, the recovery percentages were satisfactory, ranging between 71.1 % and 107.0 % (RSD ≤13.8 %). The residues ranged from below the detection level (BDL) to 14.5 μg/L. The most frequently detected pesticide was DMT (100 %), followed by CLP (52.3809–57.1429), CAR (4.7619–14.2867), and PYM (4.7619–9.5238). However, AZ and BUP were below the detection limit in the analyzed samples of both seasons. Most pesticides and the highest concentrations were detected in March 2023, while the lowest concentrations were present in August 2023.Furthermore, ecological risk assessment based on the general-case scenario (RQm) and worst-case scenario (RQex) indicated a high (RQ > 1) risk to aquatic organisms, from the presence of PYM and CLP residue in river water. Human health risk via dietary exposure was estimated using the hazard quotient (HQ). Based on the detected residues, the HQ (<1) value indicated no significant health risk. This report provides the first record of pesticide residue occurrences scenario and their impact on the river environment of Bangladesh.

Dissipation kinetics, residue level, and risk assessment of chlorantraniliprole in Rosa roxburghii and its residue removal using household decontamination technique

Rosa roxburghii (R. roxburghii) is edible and medicinal fruit rich in vitamin C. Residues and potentially ecological risks of chlorantraniliprole (CAP) in the R. roxburghii orchard have aroused concern considering its extensive use for controlling oriental fruit moth, aphid, and whitefly of R. roxburghii. In this study, an effective UPLC–MS/MS method was developed for quantitation of CAP in R. roxburghii and soil using modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) dispersive solid-phase extraction with average recoveries of 73.89–96.63% and a relative standard deviation of &lt;15%. Dissipation dynamics and terminal residue trials under the field conditions in 2021 and 2022 showed that half-lives of CAP in R. roxburghii (2.64–2.70 days) were shorter than those in soil (3.58–3.80 days), and its terminal residues in R. roxburghii and soil were 0.034–0.818 mg kg-1 and 0.003–0.015 mg kg-1, respectively. Long-term dietary and soil ecological risk assessments indicated that the risk quotient was significantly less than 100%, meaning that the use of CAP on R. roxburghii at the recommended dosage was safe to consumers and soil ecology system, and that maximum residue limits (MRLs) and safe pre-harvest intervals of CAP in R. roxburghii were recommended as 0.7 mg kg-1 and 14 days, respectively. Removal experiments of CAP residues from R. roxburghii using simple household processing approaches exhibit that 2% baking soda water had the highest removal efficiency (56.04–60.33%). This study provides the basic data for establishing MRL, the safe and rational use of CAP in R. roxburghii production as well as the household decontamination prior to consumption of R. roxburghii fruits.

Persistence of foliar applied and pre-storage seed-treated insecticides in rice and its processed products

A field study was conducted to investigate the persistence of foliar-applied thiamethoxam 25% WG at a rate of 25 g ai ha−1 and chlorantraniliprole 18.5% SC at 30 g ai ha−1 in various parts of rice plants, including whole grain rice, brown rice, bran, husk, straw, and cooked rice. Liquid Chromatography-Mass spectrometry/Mass spectrometry was used for sample analysis. Chlorantraniliprole residues were found to persist in whole grains, bran, husk, and straw at the time of harvest, while thiamethoxam residue was not detected in harvested grains, processed products, or straw. The study concluded that foliar-applied chlorantraniliprole and thiamethoxam did not pose any dietary risk in cooked rice. In a pre-storage seed treatment study, thiamethoxam 30% FS at 3 mL kg−1 was evaluated against Angoumois grain moth infestation during storage. The seeds remained unharmed for nine months and exhibited significantly less moth damage (2.0%) even after twelve months of storage. Thiamethoxam residues persisted for more than one year in whole rice grain, brown rice, bran, and husk with seed treatment, with higher residue levels observed in bran and husk. Parboiling and cooking led to the degradation of thiamethoxam residues.

Assessing the impact of Chlorantraniliprole (CAP) pesticide stress on oilseed rape (Brassia campestris L.): Residue dynamics, enzyme activities, and metabolite profiling

This study investigates the effects of chlorantraniliprole (CAP) pesticide stress on oilseed rape through comprehensive pot experiments. Assessing CAP residue variations in soil and oilseed rape (Brassia campestris L.), enzyme activities (POD, CPR, GST), and differential metabolites, we unveil significant findings. The average CAP residue levels were 18.38–13.70 mg/kg in unplanted soil, 9.94–6.30 mg/kg in planted soil, and 0–4.18 mg/kg in oilseed rape samples, respectively. Soil microbial influences and systemic pesticide translocation into oilseed rape contribute to CAP residue variations. Under the influence of CAP stress, oilseed rape displays escalated enzyme activities (POD, CPR, GST) and manifests 57 differential metabolites. Among these, 32 demonstrate considerable downregulation, mainly impacting amino acids and phenolic compounds, while 25 exhibit noteworthy overexpression, primarily affecting flavonoid compounds. This impact extends to 24 metabolic pathways, notably influencing amide biosynthesis, as well as arginine and proline metabolism. These findings underscore the discernible effects of CAP pesticide stress on oilseed rape.

Residual Pattern of Chlorantraniliprole, Thiamethoxam, Flubendiamide and Deltamethrin in Tomato Fruit and Soil.

Tomato, Lycopersicon esculentum L. is grown widely as an important day-to-day demand vegetable. The crop is attacked by various polyphagous insect pests like tomato fruit borer, stink bug, cabbage looper, flea beetle, aphids, whitefly, two-spotted spider mite, etc., and oligophagous insects like leaf-miner, five-spotted hawkmoth, etc. To combat the damage and yield loss, various chemical insecticides were sprayed on tomatoes under field conditions. The residual pattern of insecticides like chlorantraniliprole, thiamethoxam, flubendiamide, and deltamethrin residues was studied following applications of chlorantraniliprole 18.5% SC (Coragen) @ 30g a.i./ha, thiamethoxam 25% WG (Actara) @ 50g a.i./ha, flubendiamide 39.35M/M SC (Fame) @ 48g a.i./ha and deltamethrin 2.8% EC (Decis 100) @ 12.5g a.i./ha using Reverse Phase High-Performance Liquid Chromatography (RP-HPLC). Fruit samples were collected at 0 (1h after application), 1, 2, 3, 5, 7 days and at harvest time. All the residues of insecticides such as chlorantraniliprole (0.09mg kg- 1), thiamethoxam (0.03mg kg- 1), flubendiamide (0.02mg kg- 1), and deltamethrin (0.01mg kg- 1) were persisted up to 5th day. There were no residues found at harvest time. The residues of chlorantraniliprole and deltamethrin persisted up to 3rd day of spraying whereas the residues of flubendiamide and thiamethoxam were not detected on the same day in the soil.

Determination of chlorantraniliprole for managing Helicoverpa armigera and Spodoptera litura in cotton ecosystem

Cotton bollworm incidence and damages are high in India. In addition, it causes considerable yield loss. A new insecticide formulation Chlorantraniliprole 600 g/L SC was used along with recommended insecticides for managing Spodoptera litura and Helicoverpa armigera in two consecutive experimental trials during the period October 2021 to September 2022. Two foliar applications of Chlorantraniliprole (40 and 30 g a. i/ha) at ten days interval period reduced significantly the larval populations of H. armigera, S. litura without any phytotoxic symptoms in cotton. Chlorantraniliprole application in open field condition was found to be harmless to natural enemy (coccinellids and spiders). Even though, a temporary lessening of natural enemy populations was noticed after spray, progressively the population was increased within a week time. Cotton yield was high in chlorantraniliprole @ 40 g a. i/ha treated plot (22.66, 22.12 q/ha) when compared to untreated control. Similar effect was also noticed in the dose at 30 g a. i/ha (22.35, 21.81 q/ha) and it was statistically on par in both experiments. Residue analysis results confirming that, cotton lint, cotton seed and soil samples collected from treated (30 and 60 g a. i/ha) and untreated samples during harvest were free from chlorantraniliprole residues (below detectable levels of 0.008 μg/g).

Effects of Nozzle Types and Spraying Volume on the Control of Hypera postica Gyllenhal by Using An Unmanned Aerial Vehicle

In the current study, an unmanned aerial vehicle (UAV) was selected for agricultural spraying, with two nozzles, two insecticides and three spraying volumes as the spraying variables; this paper explores the impact of spraying volume on the droplet deposition in alfalfa fields. Furthermore, by comparing the control effect of spraying insecticides on alfalfa leaf weevils and the safety of pasture by UAV, the aim is to establish efficient pesticide spraying techniques for pest control in alfalfa fields, providing guidance for the green control of alfalfa leaf weevils. The effective droplet proportion of the fan-shaped nozzle (SX11001VS) was higher than that of the hollow conical nozzle (TX-VK4), and increasing the spraying volume cannot significantly improve that situation. When the spraying volume increased from 22.5 L/ha to 45.0 L/ha, the average droplet coverage, density and deposition of the two types of nozzles increased with the spraying volume. However, when the spraying volume was 30.0 L/ha and 45.0 L/ha, the average deposition of the two types of nozzles was similar. The control effect of chlorantraniliprole on alfalfa leaf weevils sprayed by using a UAV was higher than that of spinosad. There was a positive correlation between the spraying volume and the control effect, and the prevention effect of the hollow conical nozzle was better than that of the fan-shaped nozzle. The residues of chlorantraniliprole in alfalfa plants after spraying increased with the spraying volume, whether a fan-shaped nozzle or a hollow conical nozzle was used.

Development of analytical method for simultaneous determination of chlorantraniliprole and fipronil residues in Moringa leaf using LC–MS/MS and decontamination of Moringa leaves using household reagents

Moringa (Moringa oleifera, F: Moringaceae), popularly referred to as the ‘drumstick tree’ or ‘horse-radish tree’ and colloquially known as ‘Muringai’ in Tamil, is a highly sought-after crop in the Indian subcontinent for its nutritious leaves. A modified QuEChERS method was developed for accurate and simultaneous detection and quantification the residues of the two insecticides from Moringa leaves using LC–MS/MS. The method offered high degree of linearity (r2 > 0.99), recovery (70–90 %), and precision (<20%) for analysis of both the insecticides. The matrix interferences, which is a prevalent complication in the analysis of leafy and dark-pigmented matrices, was suppressed by the developed method. Three decontaminating reagents of household importance – sodium chloride, acetic acid and sodium bicarbonate – were tested for their efficacy to reduce the insecticide residue on the Moringa leaves by washing. The combination of sodium bicarbonate carbonate and sodium chloride at 5% each, was found to be the more efficacious among the treatments lowering the chlorantraniliprole residues up to 85.71% and 71.79% in case of X and 2X doses of chlorantraniliprole while the same combination lowered the concentration of fipronil to 67.95 % and 61.15 % for the two doses of the insecticide sprayed on the Moringa leaves. Moringa is an underexploited crop which has immense potential for addressing the nutritional security in the country as well as for export owing to the demand. This work attempts to address the unscientific management practices in on the crop as well as to suggest measures for minimizing the current residue loads by taking the first stride towards developing regulatory limits, which could benefit the researchers and policy makers.

Residue monitoring and dietary risk assessment of chlorantraniliprole and tolfenpyrad in Chinese kale under open‑field conditions

ABSTRACT Chlorantraniliprole and tolfenpyrad has been used for the control of diamondback moth in kale around the world with a suspension concentrate formulation being prepared for marketing in China. In order to evaluate pesticide residues in kale and intake risks for different populations, a modified QuEChERS procedure of detecting chlorantraniliprole and tolfenpyrad were simultaneously established using liquid chromatography tandem mass spectrometry (LC-MS/MS). The average recoveries of chlorantraniliprole and tolfenpyrad were in the range of 83–98%, with the precision of intra-day (n = 5) and inter-day (n = 15) ranging from 1.7 to 6.7% and from 2.1 to 4.7%, respectively. The limits of quantification were all 0.01 mg kg−1 in kale. Supervised filed trials were conducted in 12 regions of China, and results showed that residues of chlorantraniliprole and tolfenpyrad in kale were 0.01 to 0.17 and 0.01 to 0.11 mg kg−1, respectively, within the recommended pre-harvest interval (10 days). Based on these data, dietary risk assessments were performed. The long-term intake risk quotient (RQc) values for chlorantraniliprole and tolfenpyrad were 0.33% and 28.09% respectively, while the short-term risk quotient (RQa) values of the more toxic tolfenpyrad were 21% (children, 1–6 years old) and 52% (the general population, >1 years old) respectively, which were all lower than 100%, highlighting the higher chronic risk for children compared with that of the general population. However, the less than 100% value revealed that not only the long-term but also short-term risks were acceptable when consuming kale under Good Agricultural Practice conditions. The paper will be helpful in rational use of chlorantraniliprole and tolfenpyrad on kale.

Efficacy of Some Insecticides Against Thrips and Yellow Mites in Chilli

Efficacy of some insecticides new chemistry insecticides was evaluated against chilli thrips Scirtothrips dorsalis and yellow mite Polyphagotarsonemus latus, during kharif, 2019 and green and red chilli samples were subjected to residue analysis to evaluate the persistence of sprayed pesticides. Spinetoram 11.7SC at 60 g a.i./ ha was observed to be superior in managing chilli thrips with a mean reduction of 93.35% with least thrips population (0.53/ top 3 leaves) followed by cyantraniliprole 10.26OD at 60 g a.i./ ha and spirotetramat 15.31OD with 90.78 and 85.44 mean % reduction, respectively. Management of yellow mites was effective in plots treated with abamectin 1.9EC at 7 g a.i./ha of 85.73 mean per cent reduction followed by chlorfenapyr 10SC at 100 g a.i./ ha (83.48%) and emamectin benzoate 5SG at 10 g a.i./ ha (76.64%). Lambda-cyhalothrin and chlorantraniliprole residues were detected in green and red chilli among the sprayed pesticides.

Determination of chlorantraniliprole 18.5% SC in the paddy ecosystem and its risk assessment

Chlorantraniliprole belongsto theanthranilic diamide group is widely used against broad range of lepidopteron pests in a variety of vegetable and rice pests includingyellow rice stem borer and leaf folder. Supervised field trials were conducted duringRabi (2018–2019) and Kharif (2019) to evaluate the dissipation pattern and risk assessment of chlorantraniliprole 18.5% SC in paddy ecosystem following foliar application at 30 and 60 g a.i. ha-1 in two different cropping seasons.Modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) technique was used for the extraction of CAP residues with acetonitrile and determined by LC–MS/MS (ESI +).The limit of quantification (LOQ) was 0.01 µg g−1 for paddy leaf, straw, husk, and brown rice, respectively and 0.005 µg g−1 for soil. The average recoveries obtained were 84.30–88.92% from paddy leaf, 94.25–97.81% from straw, 90.21–93.38% from husk, 93.57–96.40% from brown rice and 89.93–91.14% from soil. The residues in paddy leaf dissipated within 35–40 days with a half-life of 4.33–5.07 days in Rabi and 3.92–4.86 days in Kharif at 30 and 60 g a.i. ha−1, respectively. The residues in soil dissipated within 15–21 days with a half-life of 14.44–15.75 days in Rabi and 13.33–14.44 days in Kharif at respective doses. At harvest chlorantraniliprole residues were not detected in straw, husk, and brown rice. The dietary risk of paddy leaf (green fodder) for cattle was found safe for consumption as the hazard index is less than one. Soil ecological risk assessment was found to be less than one (RQ < 0.1) for earthworms (Eisenia foetida) and arthropods (Aphidiusrhopalosiphi). The presentmethod could be useful inthe analysis ofchlorantraniliproleresidues in different cereals and vegetable crop ecosystems and application at recommended dose is safe for the final produce at harvest.

Dissipation kinetics, effect of household processing, and dietary risk assessment of the insecticide chlorantraniliprole residue in bitter gourd and soil

A single quadrupole liquid chromatography-mass spectrometry (LC-MS) method was validated to determine the insecticide chlorantraniliprole residues in bitter gourd fruit, juice, and soil, according to the SANTE guidelines. A linear curve was obtained (R2 &gt; 0.99) with LOD and LOQ at 0.003 mg kg-1 and 0.01 mg kg-1. The accuracy (87–102%) and precision (RSD &lt;5%) of the method was found to be satisfactory. The dissipation pattern of chlorantraniliprole 18.5% SC was studied by spraying twice at ten days intervals, at the recommended dose (X) (25 g a.i. ha-1), and double the recommended dose (2X) (50 g a.i. ha-1). The initial deposit on bitter gourd was 0.72 and 1.41 mg kg-1 and residues persisted up to 15 and 20 days with a half-life of 2.44 and 2.79 days at X and 2X doses, respectively. Simple decontamination techniques were found to reduce residues to the extent of 30 to 80 per cent. The reduction of chlorantraniliprole residues in bitter gourd juice was 40-50 per cent by different washing techniques. The estimated level of Risk quotient indicated (&lt;1) chlorantraniliprole residues pose no dietary risk to consumers at the level detected.

Monitoring and environmental risk assessment of agricultural fungicide and insecticides in water, sediment from Kumkale Plain, Çanakkale-Turkey

This study was conducted to assess pesticide residues in 34 water and sediment samples taken from Kumkale Plain of Çanakkale-Turkey. Residue analyses were performed with the use of Quick-Easy-Cheap-Efficient-Rugged-Safe (QuEChERS)-liquid chromatography/tandem mass spectrometry (LC-MS/MS) procedure. For method verification, blank sediment and water samples were spiked at two limits of quantification (LOQ) levels of the pesticides. Overall recovery was 81.66% for sediment and 91.50% for water samples. In sediment samples, chlorpyrifos-M had the highest concentration, pyridaben was encountered in the majority of the samples (15 samples) and the highest number of pesticides (35) was seen in sample no. s13. In water samples, methoxyfenozide had the greatest concentration, metalaxyl was encountered in the highest number of samples (three samples) and the highest number of pesticide (8) was seen in sample no.w13. Sample no. s13 and w13 were taken from around the fountain basin. Pyraclostrobin and chlorantraniliprole residues exceeded the national limits set for water. In terms of hazard quotient (HQ), pesticides in sediment and waters were found to be safe. Despite the safe nature of pesticide on samples, greater attention has been paid on toxicity of the residues. It was concluded that authorities should put strict regulations on agrochemicals to reduce health risks of these chemicals.

Dissipation kinetics and dietary risk assessment of chlorantraniliprole residues in/on garden bean (Lablab purpureus var. typicus) using ultra high-performance liquid chromatography

The aim of the study was to find out the dissipation rate of the chlorantraniliprole residues in garden bean pods. Insecticides were sprayed at the farmers’ holding located at Naraseepuram, Coimbatore during the year 2022 (March to April), and the collected samples were processed and analyzed in the laboratory. An effective modified QuEChERS method was developed and validated to estimate the chlorantraniliprole residues in Ultra High-Performance Chromatography. Good linearity with R2 greater than 0.99 was achieved with the Limit of Quantification as 0.05 mg/kg. The relative standard deviation was below 5% for the recoveries obtained (93.30 to 97.91%). The residues persisted up to 10 and 15 days after treatment for recommended (20 g a.i./ha) and double the recommended doses (40 g a.i./ha), respectively. The half-life of chlorantraniliprole in garden beans was calculated as 2.18 days for recommended dose and 2.89 days for double the recommended dose. Intake of chlorantraniliprole residues through garden beans at either of the doses was found to be safe as the calculated Risk Quotient (RQ) was less than one.

Simultaneous determination of thiamethoxam, its metabolite and chlorantraniliprole residues in paddy by quechers method

Simultaneous determination of thiamethoxam, its metabolite and chlorantraniliprole residues in paddy by quechers method

Validation and monitoring of chlorantraniliprole residues in paddy straw, paddy whole grain, unpolished rice and husk using LC-MS/MS

Validation and monitoring of chlorantraniliprole residues in paddy straw, paddy whole grain, unpolished rice and husk using LC-MS/MS

Development and validation of integrated pest management modules against spotted pod borer Maruca vitrata Fabricius on garden bean Lablab purpureus var. typicus (L.)

To reduce the detrimental effect of insecticides, an effective Integrated Pest Management (IPM) module is necessary for the eco-friendly management of Maruca vitrata in garden bean ecosystem. Two field trials were carried out to evaluate the efficacy of different insecticides and botanicals against M. vitrata on Lablab purpureus var. typicus. Two seasons field evaluation of insecticides revealed that chlorantraniliprole 18.5 SC was the most effective treatment to control the pest recorded 0.11 and 0.36 larva/plant in two seasons, respectively after two rounds of spray followed by flubendiamide 20 WG (0.46 and 0.92 larva/ plant) and emamectin benzoate 5 SG (0.50 and 0.95 larva/plant). Among botanicals tested, commercial neem formulation and 5% Ageratina adenophora recorded the least larval count of 1.64 &amp; 1.05 larva/plant and 2.24 &amp; 1.45 larva/plant in two seasons, respectively. IPM modules were developed with three effective insecticides (chlorantraniliprole 18.5 SC, flubendiamide 20 WG and emamectin benzoate 5 SG), two effective botanicals (commercial neem formulation 1500 ppm and 5% A. adenophora) along with the pheromone trap for validation. All the IPM modules were equally effective in managing M. vitrata population on L. purpureus and recorded a significantly (at 5 %) lower larval population than the farmer’s practice. The residues of chlorantraniliprole, flubendiamide and emamectin benzoate reached below the detectable level at the time of harvest. The population reduction of predatory coccinellids and spiders was also lower in IPM modules than in farmer’s practice. An increased benefit cost (1.95 to 1.99) ratio was observed in IPM modules.

Determination of Chlorantraniliprole Residue in Soil by Gas Chromatography-Mass Spectrometry

Determination of Chlorantraniliprole Residue in Soil by Gas Chromatography-Mass Spectrometry

Determination of Chlorantraniliprole Residue in Soil by Gas Chromatography-Mass Spectrometry

Gas chromatography-mass spectrometry method was developed and validated for the quantification of anthranilic diamide insecticide chlorantraniliprole residues in soil. The soil samples were extracted with acetonitrile and dispersive solid phase extraction clean up. The average recoveries of chlorantraniliprole were in the range of 94.10-96.82% with reproducibility and repeatability was ranged between 0.17 to 1.90 and 0.97 to 1.22%, respectively. The limit of quantification of the analytical method was 0.01 μg g-1 and the matrix effect was less than 1%.