Chloramphenicol In Water Samples Research Articles

Core-shell magnetic molecularly imprinted polymers prepared by self-polymerization of bifunctional monomers for efficient enrichment and separation of chloramphenicol in water samples

Chloramphenicol (CAP) is a potent antibiotic used to treat infections in livestock and humans, but its residues can be hazardous to human health. In this study, amino-modified Fe3O4 nanoparticles were prepared by a single-pot method, and magnetic molecularly imprinted materials were prepared by self-polymerization for imprinting with CAP. The imprinted thin layer was prepared by polymerization of dopamine (DA) and 3-aminophenylboronic acid (APBA) copolymers, which improved the elution efficiency of the template. Dopamine generated under alkaline conditions replaces the crosslinker and can be adsorbed on the surface of magnetic carriers. The aromatic groups in dopamine make it difficult to degrade and fall off after adsorption on the magnetic carrier surface. The maximum adsorption amount of CAP on magnetic materials was 6.5 mg/g. After several adsorption cycles, the CAP-MMIPs still had good stability. The results showed that the method can both specifically recognize and enrich the separation of the target antibiotics, which greatly reduced the pretreatment time of the samples and provided a new approach for the pretreatment of CAP.

Highly sensitive and selective electrochemical sensor for the determination of chloramphenicol

Recently, there has been significant interest in the utilization of indium-doped cadmium sulfide (CdS) for the detection of chloramphenicol (CAP) in food samples due to its exceptional performance. The objective of this research is to develop a simple, rapid, and highly sensitive sensor for CAP detection. CdS possesses remarkable characteristics as a semiconductor material, including a large specific surface area, abundant pore structure, and excellent electrical conductivity. The electrochemical properties of glassy carbon electrodes (GCE) were comprehensively investigated both in their pristine state and when modified with CdS/GCE, CdIn2S4/GCE, Cd1.5In2S4.5/GCE, and Cd2In2S5/GCE. The surface areas of the modified electrodes CdIn2S4/GCE, Cd1.5In2S4.5/GCE, and Cd2In2S5/GCE were measured to be 0.08 cm2, 0.097 cm2, and 0.25 cm2, respectively. Five replicate samples using the optimized proportioned electrode Cd2In2S5/GCE resulted in a response error below 5%. These results indicate that the composite demonstrates exceptional conductivity and reproducibility while maintaining satisfactory sensitivity and high specificity. The proposed sensor based on Cd2In2S5/GCE exhibited an impressive dynamic linear range from 10 nmol/L to 20 μmol/L with an exceptionally low detection limit of 3.8 nmol/L and remarkable sensitivity of 0.38 μA μm−1 cm−2. This sensor successfully quantified CAP in water samples with a satisfactory recovery rate ranging from 99.5% to 100.3%, accompanied by an RSD below 7.6%. Consequently，this developed electrochemical sensor presents itself as the optimal choice for simultaneous determination of CAP in both food and water samples.

Magnetic nanoparticles assisted dispersive liquid-liquid microextraction of chloramphenicol in water samples.

This work describes the development of a new methodology based on magnetic nanoparticles assisted dispersive liquid–liquid microextraction (DLLME-MNPs) for preconcentration and extraction of chloramphenicol (CAP) antibiotic residues in water. The approach is based on the use of decanoic acid as the extraction solvent followed by the application of MNPs to magnetically retrieve the extraction solvent containing the extracted CAP. The coated MNPs were then desorbed with methanol, and the clean extract was analysed using ultraviolet–visible spectrophotometry. Several important parameters, such as the amount of decanoic acid, extraction time, stirring rate, amount of MNPs, type of desorption solvent, salt addition and sample pH, were evaluated and optimized. Optimum parameters were as follows: amount of decanoic acid: 200 mg; extraction time: 10 min; stirring rate: 800 rpm; amount of MNPs: 60 mg; desorption solvent: methanol; salt: 10%; and sample pH, 8. Under the optimum conditions, the method demonstrated acceptable linearity (R2 = 0.9933) over a concentration range of 50–1000 µg l–1. Limit of detection and limit of quantification were 16.5 and 50.0 µg l–1, respectively. Good analyte recovery (91–92.7%) and acceptable precision with good relative standard deviations (0.45–6.29%, n = 3) were obtained. The method was successfully applied to tap water and lake water samples. The proposed method is rapid, simple, reliable and environmentally friendly for the detection of CAP.