Seroprevalence Of Chlamydia Trachomatis Research Articles

Seroprevalence of sexually transmitted infections over 44 years - A cross-sectional study in Sweden.

Sexually transmitted infections (STIs) may cause substantial individual suffering and a large economic burden for society. This study examined the seroprevalence of Chlamydia trachomatis, Mycoplasma genitalium, herpes simplex virus (HSV) types 1 and 2, and several human papillomaviruses (HPV) in the Swedish population over time. The study population consisted of 30-year-old women attending maternity care, and 50year-old men and women attending health check-ups, from 1975 to 2018. Antibody status was determined by multiplex serology and quantified using median reporter fluorescence intensity (MFI). A total of 891 samples were analysed (519 from 30-year-old women, 186 from 50year-old women and 186 from 50year-old men). Of these, 41.5% showed seropositivity for Chlamydia trachomatis, 16.7% for Mycoplasma genitalium, 70.5% for HSV-1, 14.9% for HSV-2, 13.2% for high-risk HPV, and 8.3% for low-risk HPV. Seropositivity for Mycoplasma genitalium, HSV-1 and especially Chlamydia trachomatis decreased over time. There was a decrease over time in Chlamydia trachomatis seroprevalence, probably due to contact tracing, testing and early treatment; this might also have affected Mycoplasma genitalium seroprevalence. Despite the reduction, seroprevalences are still high, so continued and new efforts to reduce STI incidence are essential.

Seroprevalence of Chlamydia trachomatis and associated risk factors among HIV positive women in North Central Nigeria.

Chlamydia trachomatis infection is among the STDs that are known to increase the risk of HIV infection. The present study aims to determine the seroprevalence of C. trachomatis among HIV positive women in Ilorin and Offa, Kwara State, North Central Nigeria. Serum samples from 400 HIV positive women attending the HAART Clinic in Offa and the Ilorin General Hospital in Kwara State, Nigeria, were screened using Enzyme Linked Immunosorbent Assay (ELISA), utilizing the immunocomb Chlamydia IgG test kit (Calbiotech, El Cajon, CA, USA) to check for the existence of anti-C. trachomatis antibodies. Anti-C. trachomatis antibodies were present in 92 (23.0%) of the 400 HIV positive women samples. There was a higher prevalence among the age group 36-40 years. Hence, age groupings were statistically and significantly associated (p=0.001) with the seroprevalence of C. trachomatis among HIV positive women. Married HIV positive women (60.9%) had the highest prevalence of C. trachomatis, with a statistically significant association (p=0.001). There was a statistically significant association between the number of sexual partner(s) (p=0.001) and the seroprevalence of C. trachomatis among HIV positive women. The high frequency confirms the necessity for comprehensive sexual education among young adults and routine testing for anti-C. trachomatis. It reflects the endemicity of the infection in Ilorin and Offa Kwara State, Nigeria.

Sero-Prevalence of Chlamydia trachomatis among HIV Patients and Healthy Female Volunteers in Port-Harcourt, Rivers State, Nigeria

Background: Chlamydios is caused by the bacterium Chlamydia trachomatis is the most prevalent bacterial Sexually Transmitted Infections (STI) known to cause damage to a woman’s reproductive organs. The study was undertaken to determine the sero-prevalence of chlamydiosis among HIV patients and Healthy female volunteers in Port-Harcourt. The prevalence of chlamydiosis was investigated among 250 (150 HIV patients, and 100 Healthy volunteers) consented women of reproductive age attending Rivers State University Teaching Hospital in Port-Harcourt, Rivers State. Enzyme Linked Immunosorbent assay technique was performed to detect Chlamydia trachomatis IgG antibodies. The overall sero-prevalence of C. trachomatis in the among healthy volunteers was 10%, while that of HIV patents was 12.7%. HIV patients who were above 35 years accounted for 16% of the disease distribution. When prevalence was evaluated with the subgroups, there was no significant difference of Chlamydia trachomatis IgG antibodies across the subgroups (P>0.05). There was no association between socio-demographic factors and sero-prevalence of chlamydiosis. The possible risk factor for chlamydiosis base on life style was lack of screening for Chlamydia trachomatis (P<0.05) as a result of limited awareness of the infection by majority of the participants. Chlamydiosis is largely under diagnosed in the population. Therefore every sexually active woman irrespective of age is expected to undergo screening for chlamydia infection.

Seroprevalence of Chlamydia trachomatis and Associated Factors among Vulnerable Riverine in the Brazilian Amazon

Due to social and individual conditions and access to health services, Amazonian riverside populations are highly vulnerable to sexually transmitted infections, including Chlamydia trachomatis. The aim is to estimate the seroprevalence of Chlamydia trachomatis and analyze the associated factors among riverside dwellers in a capital city in the Brazilian Amazon. A cross-sectional study was carried out with residents of the Combu Island, Belém. The study sample was calculated using the population survey technique in the EPI INFO. Only people aged 18 and over were included. ELISA serology was performed to detect antibodies against Chlamydia trachomatis. For data collection, a form containing vulnerability factor questions was applied. Binary regression analysis was performed using the Minitab 20 program. The study sample consisted of 325 participants. The prevalence of IgG/IgM antibodies against Chlamydia trachomatis was 22.2% and 5.5%, respectively. In the multiple regression, only participants with a broken condom were more likely to have antibodies against the bacteria (OR: 1.90; 95% CI: 1.01; 3.37; p = 0.046). Seroprevalence was associated with condom breakage. This factor demonstrates that despite having an attitude towards condom use, probably, they may have inadequate knowledge about the correct practice of introduction.

Distribution Rate of Chlamydial Infection According to Demographic Factors among Pregnant Women Attending Clinics in Zaria Metropolis, Kaduna State, Nigeria

Chlamydia trachomatis also known as the “Silent Epidemic” is a major threat to the reproductive health of women. This study was aimed at determining the seroprevalence of Chlamydia trachomatis based one demographic factors among women attending clinics in Zaria metropolis, Kaduna State. Each participant completed a researcher-devised questionnaire and quasi design was used in the selection of hospitals. Subsequently about 5mls of peripheral blood for serological analysis was obtained after informed consent. Presence of antibodies to Chlamydia trachomatis was determined using Enzyme Linked Immunosorbent Assay (ELISA) to detect IgG and screening for HIV was also done using Determine® HIV 1/2 as well as Uni-GoldTM HIV Test Kits. Out of the two hundred and seventy (270) samples collected, 32(11.9%) were positive for Chlamydia trachomatis IgG, 7(2.6%). Chlamydial infection was found to be significantly associated with level of education. There was no significant association between chlamydial infection and occupation, subjects’ husbands’ occupation.

Effect of chlamydia trachomatis on infertile women

Objective: This study was carried out to determine the seroprevalence of Chlamydia trachomatis in hospitalized women has been made. Materials and methods: Chlamydial antigen and anti-chlamydial antibodies were investigated in 145 patients who applied to Al Khanasaa teaching hospitals in City of Mosul-Iraq and were diagnosed as infertile, and in 81 women who gave birth. Results: Endocervical chlamydial antigen in one (0.7%) patient in the infertile group; While anti-chlamydial antibody was positive in 7 (4.8%) patients, endocervical chlamydial antigen and anti-chlamydial antibody positivity were not detected in fertile cases. Conclusion: Seropositivity of 5.5% (seven antibody positive and one antigen positive case) was obtained in 145 infertile women; In infertile cases, antibody positivity intensifies between the ages of 25-35; It was determined that a case with positive chlamydial antigen was 20 years old.

Effect of chlamydia trachomatis on infertile women

Objective: This study was carried out to determine the seroprevalence of Chlamydia trachomatis in hospitalized women has been made. Materials and methods: Chlamydial antigen and anti-chlamydial antibodies were investigated in 145 patients who applied to Al Khanasaa teaching hospitals in City of Mosul-Iraq and were diagnosed as infertile, and in 81 women who gave birth. Results: Endocervical chlamydial antigen in one (0.7%) patient in the infertile group; While anti-chlamydial antibody was positive in 7 (4.8%) patients, endocervical chlamydial antigen and anti-chlamydial antibody positivity were not detected in fertile cases. Conclusion: Seropositivity of 5.5% (seven antibody positive and one antigen positive case) was obtained in 145 infertile women; In infertile cases, antibody positivity intensifies between the ages of 25-35; It was determined that a case with positive chlamydial antigen was 20 years old.

Genital Chlamydia trachomatis Seroprevalence and Uterine Fibroid Development: Cohort Study of Young African-American Women.

Few studies have investigated the 1930s hypothesis that reproductive tract infections are risk factors for fibroid development. In our 2017 cross-sectional analysis from the Study of Environment, Lifestyle, and Fibroids (2010–2018), a large Detroit community-based cohort of 23–35 year-old African-American women with ultrasound fibroid screening, we found an inverse association between seropositivity for genital Chlamydia trachomatis (gCT) infection and fibroids. With prospective data from the cohort (standardized ultrasounds every 20 months over 5 years), we examined gCT’s associations with fibroid incidence (among 1158 women fibroid-free at baseline) and growth. We computed adjusted hazard ratios (aHRs) and 95% confidence intervals (CIs) for incidence by gCT serostatus using Cox proportional hazards models. GCT’s influence on growth was assessed by estimating the difference between fibroid size change for seropositive vs. seronegative between successive ultrasounds (1254 growth measures) using a linear mixed model. Growth was scaled to change over 18 months. GCT seropositivity was not associated with fibroid incidence (aHR, 1.0 95% CI: 0.79, 1.29) or growth (4.4%, 95% CI: −5.02, 14.64). The current evidence based on both biomarker gCT data, which can capture the common undiagnosed infections, and prospective ultrasound data for fibroids suggests that Chlamydia is unlikely to increase fibroid risk.

Seroprevalence of Chlamydia trachomatis, herpes simplex 2, Epstein-Barr virus, hepatitis C and associated factors among a cohort of men ages 18-70 years from three countries.

To estimate the seroprevalence of Chlamydia trachomatis (CT), herpes simplex type-2 (HSV2), hepatitis C (HCV), Epstein-Barr virus (EBV) and nine human papilloma virus (HPV) types, and investigated factors associated with the seropositivity among men from three countries (Brazil, Mexico and U.S). Archived serum specimens collected at enrollment for n = 600 men were tested for antibodies against CT, HSV2, HCV, EBV, and 9-valent HPV vaccine types (6/11/16/18/31/33/45/52/58) using multiplex serologic assays. Socio-demographic, lifestyle and sexual behavior data at enrollment were collected through a questionnaire. Overall, 39.3% of the men were seropositive for CT, 25.4% for HSV2, 1.3% for HCV, 97.3% for EBV, 14.0% for at least one of the seven oncogenic HPV (types: 16/18/31/33/45/52/58), and 17.4% for HPV 6/11. In the unadjusted models, age, race, smoking, sexual behavior variables, and seropositivity for high-risk HPV were significantly associated with the seropositivity for CT. In multivariable analyses, self-reported black race, higher numbers of lifetime female/male sexual partners, current smoking, and seropositivity to high-risk HPV were significantly associated with increased odds of CT seropositivity. Odds of HSV2 seroprevalence were elevated among older men and those seropositive for high risk HPV. Exposure to STIs is common among men. Prevention and screening programs should target high-risk groups to reduce the disease burden among men, and to interrupt the disease transmission to sexual partners.

Differences in Chlamydia trachomatis seroprevalence between ethnic groups cannot be fully explained by socioeconomic status, sexual healthcare seeking behavior or sexual risk behavior: a cross-sectional analysis in the HEalthy LIfe in an Urban Setting (HELIUS) study

BackgroundIn the Netherlands, there are strong disparities in Chlamydia trachomatis (CT) prevalence between ethnic groups. The current study aims to identify whether socioeconomic status, sexual risk behavior and sexual healthcare seeking behavior may explain differences in CT seroprevalence between ethnic groups.MethodsWe used 2011–2014 baseline data of the HELIUS (HEalthy LIfe in an Urban Setting) study, a multi-ethnic population-based cohort study in Amsterdam, the Netherlands, including participants from Dutch, African Surinamese, South-Asian Surinamese, Ghanaian, Moroccan and Turkish origin. For this analysis, we selected sexually active, heterosexual participants aged 18–34 years old. CT seroprevalence was determined using a multiplex serology assay. The CT seroprevalence ratios between different ethnicities are calculated and adjusted for potential indicators of socioeconomic status, sexual risk behavior and sexual healthcare seeking behavior.ResultsThe study population consisted of 2001 individuals (52.8% female) with a median age of 28 years (IQR 24–31). CT seropositivity differed by ethnicities and ranged from 71.6% (African Surinamese), and 67.9% (Ghanaian) to 31.1% (Turkish). The CT seroprevalence ratio of African Surinamese was 1.72 (95% CI 1.43–2.06) and 1.52 (95% CI 1.16–1.99) of Ghanaian as compared to the Dutch reference group, after adjustment for socioeconomic status, sexual risk behavior and sexual healthcare seeking behavior.ConclusionsIndicators of socioeconomic status, sexual risk behavior, and sexual health seeking behavior could not explain the higher CT seroprevalence among African Surinamese and Ghanaian residents of Amsterdam.

Seroprevalence of Chlamydia trachomatis Among Sexually Active Women in Osun State

Seroprevalence of Chlamydia trachomatis Among Sexually Active Women in Osun State

A Serological Survey of Chlamydia trachomatis and its Related Factors in Individuals with High-Risk Sexual Behavior.

Chlamydia trachomatis is the most common bacterial sexually transmitted infection (STI) throughout the world and its annual incidence is reported to be around 50 million cases. High-risk sexual behaviors are among the predisposing factors for STIs. The present study aimed to determine the seroprevalence of Chlamydia trachomatis and the factors affecting it in patients with high-risk sexual behaviors who had attended clinic of high risk sexual behavior of Urmia between October 2015 and June 2016. This was a cross-sectional study conducted at Behavior Disorders Clinic in Urmia. One hundred and seventy six patients who had attended the clinic were selected using convenient sampling. A questionnaire was filled out by the participants and a 5 cc sample of their blood was collected to determine their serological level of antibodies. The participants' blood serum samples were analyzed by ELISA. Descriptive tests, T-test, and Chi-square test were run to analyze the data. All the statistical analyses were done using Statistical Package for the Social Sciences (SPSS) software, version 23. The results indicated that 52.3% of the attendees of the Behavior Disorders Clinic in Urmia were single, 78.4% had a sexual partner except from their spouse, 31.8% had multiple sexual partners, and 8.52% had a high-risk partner. The results also revealed that there was a significant relationship between gender and presence of anti-Chlamydia antibodies (p=0.02). However, no significant relationship was observed between other demographic characteristics or history of high-risk behaviors and presence of anti- Chlamydia antibodies. Since the frequency of positive IgM was 11.9% in the present study, and Chlamydia trachomatis infections are mostly asymptomatic, early diagnosis and treatment of this pathogen can have a very important role in public health improvement. Screening individuals with high-risk sexual behavior is recommended.

Chlamydia trachomatis Seroprevalence and Ultrasound-Diagnosed Uterine Fibroids in a Large Population of Young African-American Women

Chlamydia trachomatis Seroprevalence and Ultrasound-Diagnosed Uterine Fibroids in a Large Population of Young African-American Women

Chlamydia trachomatis Seroprevalence and Ultrasound-Diagnosed Uterine Fibroids in a Large Population of Young African-American Women.

Reproductive tract infections have long been hypothesized to increase the risk of uterine fibroids. Few studies have been conducted, even for the common infection genital Chlamydia trachomatis (gCT), and only with self-reported gCT data. Our investigation used micro-immunofluorescence serology for gCT to characterize past exposure. We used cross-sectional enrollment data from a prospective fibroid study carried out in the Detroit, Michigan, area; ultrasound examinations systematically screened for fibroids. Participants were African-American women aged 23-34 years (recruited in 2010-2012). Age- and multivariable-adjusted logistic regression models were used to estimate odds ratios. A total of 1,587 women (94% of participants) had unequivocal gCT serology results; 22% had fibroids. Those who were seropositive for gCT were less likely to have fibroids (age-adjusted odds ratio = 0.68, 95% confidence interval: 0.54, 0.87; multivariable-adjusted odds ratio = 0.80, 95% confidence interval: 0.62, 1.03). Inverse associations were similar across categories of fibroid size, number, and total volume. Participant groups likely to have had multiple or severe infections (multiple serovar groups, more sex partners, clinically diagnosed chlamydia) all showed statistically significantly reduced odds of fibroids. A protective association of gCT with fibroids was unexpected but plausible. gCT infection might increase immune surveillance and eliminate early lesions. Further investigation on the relationship between fibroid development and reproductive tract infections is needed.

Seroprevalence of Chlamydia trachomatis (CT) in Inner City Children and Adolescents: Implications for Vaccine Development

Seroprevalence of Chlamydia trachomatis (CT) in Inner City Children and Adolescents: Implications for Vaccine Development

Seroprevalence of Chlamydia trachomatis, cytomegalovirus, herpes simplex virus 1 and 2 in Saudi women with normal and abnormal early pregnancy: A case control study

This work measured the seroprevalence of Chlamydia trachomatis, cytomegalovirus, herpes simplex virus-1 and 2 in Saudi women with normal and abnormal early pregnancy. This is a case-control study; serum samples were collected from 250 women as follows: 100 normal pregnancies, 70 ectopic pregnancies and 80 spontaneous abortions. IgG and IgM antibodies against candidate organisms were measured using enzyme linked immunosorbant assay (ELISA). The prevalence of C. trachomatis IgG, but not IgM, was significantly higher in ectopic pregnancy (18.5%) and abortion (10%) as compared to normal pregnancy (5%) (P < 0.05). The frequency of HSV-1 IgG, but not HSV-2, was also higher in ectopic pregnancy (94.3%) and abortion (87.5%) than in normal pregnancy (64%). However, the rate of detecting both IgM and IgG antibodies together either against HSV-1 or HSV-2 was higher in the ectopic (41.1 and 30%, respectively) and abortion groups (28.7 and 36.2%, respectively) as compared to the control (9 and 16%, respectively). Correspondingly, the frequency of cytomegalovirus (CMV) IgG was significantly higher in ectopic pregnancy (91.4%) and abortion (75%) as compared to normal pregnancy (58%). Similar results were also observed for CMV IgM antibodies either alone or with IgG antibodies in the ectopic and abortion groups as compared to control (P < 0.05). Candidate organisms were common by serology in Saudi females with abnormal early pregnancy. Additionally, the high frequency rate in normal pregnancy alarms the potential high risk of congenital infection. Further studies are needed to measure the true prevalence of these sexually transmitted infections (STIs) and their associated pregnancy complications in Saudi Arabia. Key words: Chlamydia trachomatis, cytomegalovirus, herpes simplex virus, ectopic pregnancy, abortion, Saudi Arabia.

English

INTRODUCTION: Chlamydia trachomatis is the leading cause of sexually transmitted infections (STIs). Chlamydial infections, if undiagnosed and untreated can result in irreversible sequelae. Infected patients serve as a reservoir of infection to their partners. The present study was designed to determine the epidemiological markers and seroprevalence of genital Chlamydia in female STI patients attending STI clinics. METHODS: A total of 226 clinically suspected cases of Sexually Transmitted Infection (STI) patients attending STI clinic were studied for seroprevalence of chlamydia trachomatis along with age, marital status, history of sexual contact and contraception use. Patients were investigated for the presence of IgG antibody of chlamydia trachomatis with ELISA (Novatech, Germany). RESULTS: Seroprevalence of chlamydia trachomatis was found to be 55.66% for IgG by ELISA. Genital Chlamydia was more commonly seen in sexually active group (21-30 years).Highest prevalence was seen in married patients (53.75%), in patients who had history of sexual contact (61.25%) and who were using oral contraceptive pills (63.93%) as a contraceptive method. CONCLUSION: Though tissue culture is gold standard, serological assays are much simpler, sensitive and rapid methods for detection of chlamydia trachomatis. Co-infection of Chlamydia with other STIs highlights the importance of early laboratory diagnosis and specific treatment.

Seroprevalence of Chlamydiatrachomatis among HIV Positive Women in Bida, North Central Nigeria

Seroprevalence of Chlamydiatrachomatis among HIV Positive Women in Bida, North Central Nigeria

An Increasing Proportion of Reported Chlamydia trachomatis Infections Are Repeated Diagnoses

In recent decades, increasing rates of Chlamydia cases have contrasted with decreasing Chlamydia trachomatis seroprevalence rates and decreasing Chlamydia-associated complication rates. We elucidated the conflicting trends by studying incidence of repeated Chlamydia infections over time. Chlamydia cases reported during 1995 to 2009 were identified in the Finnish National Infectious Diseases Registry. Trends of single and repeated diagnoses of Chlamydia infection were analyzed. Our study population comprised 147,148 individuals with a total of 177,138 genital chlamydial infections. The proportion of annual repeated diagnoses of genital infections increased among female and males from 4.9% to 7.3% and from 3.8% to 5.3%, respectively. In 2009, 24.8% of the females and 20.3% of the males had had an earlier Chlamydia infection ever during the follow-up time. Of all the repeated diagnoses, 34.1% occurred within 12 months. The highest rates of repeated infection diagnoses occurred in 25-year-old women (37.0%) and in 29-year-old men (30.9%) in a cohort of individuals born in 1979. A gradual increase of repeated Chlamydia infections resulted in 43% increase in annual infections between 1996 and 2009. The result is supportive of the existing seroprevalence data suggesting that Chlamydia infection burden is not increasing in the whole population. The increasing infection rates in males, in particular, justify development of effective strategy in preventing reinfections and onward transmission.

Seroprevalence of Chlamydia trachomatis in Enugu, Nigeria

Chlamydia infections in women cause pelvic inflammatory disease, which often results in devastating consequences of infertility, ectopic pregnancy, or chronic pelvic pain. The infection is largely asymptomatic. To determine the seroprevalence of Chlamydia trachomatis in Enugu, South Eastern Nigeria. A population-based prospective study comprising female residents of Enugu, South Eastern Nigeria. Indirect solid phase enzyme immunoassay of Chlamydia antibodies was done using ImmunoComb C0. Trachomatis IgG Kit (Orgenics). The population comprised 136 female undergraduate students and 150 non-student women. The overall prevalence of C. trachomatis in the population studied was 29.4%. The percentage of subjects who admitted to be having multiple sexual partners was higher among the student population (71.2%) compared to those from the non-student population (28.8%). The highest percentage of seroprevalence was 28 (33.3%) in the age group of 20-24 years for the student population and 18 (21.4%) in the age group of 25-29 years for the non-student population. The highest seroprevalence of C. trachomatis antibodies (69.0%) in both populations was observed in females without any history of infection. Females that had pelvic inflammatory disease, sexually transmitted infection, and secondary infertility assayed for C. trachomatis had seroprevalence levels of 19%, 9.5%, and 2.4%, respectively. There was a positive correlation between positive Chlamydia assay and the type of subject population (student or non-student) with r2 value of 1.55 at P < 0.01. C. trachomatis infection is largely underdiagnosed and remains a silent disease in the apparently healthy population of Enugu, South eastern Nigeria.