Chinese Cabbage Seedlings Research Articles

Integrated Analysis of Metabolome and Transcriptome Reveals the Effect of Burdock Fructooligosaccharide on the Quality of Chinese Cabbage (Brassica rapa L. ssp. Pekinensis).

Burdock fructooligosaccharide (BFO) is fructose with a low polymerization degree, which could improve the immunity to pathogens, quality, and stress resistance of vegetables. Still, there are no studies on applying BFO in Chinese cabbage. In this study, the effects of exogenous BFO sprayed with different concentrations (0, 5, 10, 20, 30 g·L-1) on the growth and soluble sugar content of Chinese cabbage seedlings were determined. The result showed that 10 g·L-1 was the appropriate spraying concentration. Based on metabolome analysis, a total of 220 differentially accumulated metabolites (DAMs) were found, among which flavonoid metabolites, glucosinolate metabolites, and soluble sugar-related metabolites were the key metabolites involved in improving the quality of Chinese cabbage caused by BFO. Further combination analysis with transcriptome, trans-cinnamate 4-monooxygenase (CYP73A5), and chalcone synthase 1 (CHS1) were more closely associated with the DAMs of flavonoid biosynthesis. Sulfotransferases 18 (SOT18), Branched-chain amino acid amino transferases 6 (BCAT6), and cytochrome P450 monooxygenase (CYP83A1) were the key genes in glucosinolate biosynthesis. Hexokinase (HxK1), beta-glucosidase 8 (BGL08), invertase 3 (INV3), beta-glucosidase 3B (BGL3B), and sucrose phosphate synthase 1 (SPS1) were significantly upregulated, potentially playing crucial roles in the soluble sugar metabolism. In conclusion, these results provided an understanding of the effects of BFO on the expression of genes and the accumulation of metabolites related to quality formation in Chinese cabbage.

Effect of sodium selenite on the synthesis of glucosinolates and antioxidant capacity in Chinese cabbage (Brassica rapa L.ssp.pekinensis).

Chinese cabbage (Brassica rapa ssp. pekinensis) is a globally cultivated and consumed leafy vegetable due to its abundant plant secondary metabolites and antioxidant compounds, including flavonoids, ascorbic acids, glucosinolates, and vitamins, which have been reported to confer health-promoting effects. Glucosinolates components in leaves of Chinese cabbage plantlets under different concentrations of sodium selenite (0, 30, and 50μmol/L) were analyzed. Seven glucosinolates were identified and quantified using UHPLC-QTOF-MS. Finally, treatments with 30 and 50μmol/L Na2SeO3 solution significantly increased the levels of total selenium content as well as total phenols, flavonoids, anthocyanins, and DPPH free radical scavenging ability in Chinese cabbage seedlings. Our results revealed that 30μmol/L Na2SeO3 effectively enhanced aliphatic glucosinolate levels and total glucosinolate content while causing a significant reduction in indole glucosinolates. Furthermore, downregulation was observed for BrCYP79F1, BrBCAT4, and BrMAM1 genes associated with aliphatic glucosinolate synthesis. Conversely, BrMYB28 and BrCYP83A1 genes exhibited significant upregulation. Thus, the positive influence of Na2SeO3 on glucosinolate biosynthesis in Chinese cabbage can be attributed to the upregulation of key genes related to this process.

Establishment of an Efficient Screening Methods for Resistance of Chinese Cabbage to Clubroot Disease

Clubroot caused by <i>Plasmodiophora brassicae</i> is an important disease of crucifer crops worldwide. This study aimed to establish an efficient screening method to determine resistant cultivars of Chinese cabbage against <i>P. brassicae</i>. To do this, we investigated the virulence of seven <i>P. brassicae</i> isolates using seedlings of susceptible Chinese cabbage cultivar. The isolates exhibited different virulence in the plants and were divided into three groups based on their virulence. When we explored the disease occurrence in Chinese cabbage seedlings according to photoperiod after inoculation of <i>P. brassicae</i> and incubation temperature, the plants with all-day light showed higher disease severity than seedlings cultivated under 14 hr of light a day. The occurrence of clubroot disease was most severe at 25<sup>o</sup>C, followed by 20<sup>o</sup>C and 18<sup>o</sup>C, but the fresh weight of clubroot of the seedlings cultivated at 20<sup>o</sup>C was the highest, followed by plants grown at 25<sup>o</sup>C and 18<sup>o</sup>C. When the seedlings of two commercial resistant cultivars were inoculated with the mixed spore suspensions of two different pathotype isolates of <i>P. brassicae</i>, disease severity increased as the spore concentration of the susceptible <i>P. brassicae</i> isolate among the two strains increased, suggesting that the clubroot development by different pathotype isolates was independent and not influenced by each other. Taken together, our results provide a faster and more accurate screening methods to determine the resistance of Chinese cabbage against <i>P. brassicae</i>.

Inoculation of Paraburkholderia sp. GD17 improves seedling growth and tolerance to Cadmium in Chinese cabbage

This study provided evidence at the first time showing that root inoculation with the plant growth-promoting rhizobacteria Paraburkholderia sp. GD17 improved the growth and tolerance to Cd stress in Chinese cabbage seedlings. Under normal conditions, the shoot fresh and dry weight of GD17-inoculated 30-day-old plants increased by about 29 % and 33 %, and their root fresh and dry weight by 104 % and 67 %, respectively, compared with their non-inoculated partners. The GD17-mediated growth promotion could be attributed to its facilitating influence on plant acquisition of nutrient elements and photosynthetic efficiency, and decreasing abscisic acid production. Under Cd stress, an effective alleviation in Cd-induced growth inhibition was observed in GD17 plants relative to non-inoculated control, suggesting that the root inoculation with GD17 played a systemic protective role. The Cd concentration in plant aerial tissues was comparable between GD17 plants and non-inoculated ones, but it was substantially decreased in GD17 plant roots. In response to Cd, GD17-inoculated plants generally showed a stronger ability to absorb and transport nutrient elements to shoots. The GD17-conferred plant tolerance to Cd was also associated with an increased antioxidative capacity companied by declined oxidative damage, optimal levels of phytohormones, increased flavonoid synthesis as indicated by significantly upregulated expression of related genes and activity of phenylalanine ammonia-lyase. Additionally, root inoculation with GD17 effectively mitigated the Cd-induced decline in photosynthetic efficiency. Collectively, this study firstly showed that GD17-conferred growth-promotion and Cd-tolerance in Chinese cabbage was correlated with multiple regulatory roles in plant metabolism, which, in most cases, was involved in the regulation at the transcription levels of relevant genes.

Improved YOLO v7 for Sustainable Agriculture Significantly Improves Precision Rate for Chinese Cabbage (Brassica pekinensis Rupr.) Seedling Belt (CCSB) Detection

Precise navigation in agricultural applications necessitates accurate guidance from the seedling belt, which the Global Positioning System (GPS) alone cannot provide. The overlapping leaves of Chinese cabbage (Brassica pekinensis Rupr.) present significant challenges for seedling belt fitting due to difficulties in plant identification. This study aims to address these challenges by improving the You Only Look Once (YOLO) v7 model with a novel approach that decouples its network head deriving from the Faster-Regions with Convolutional Neural Network (Faster R-CNN) architecture. Additionally, this study introduced a BiFormer attention mechanism to accurately identify the centers of overlapping Chinese cabbages. Using these identified centers and pixel distance verification, this study achieved precise fitting of the Chinese cabbage seedling belt (CCSB). Our experimental results demonstrated a significant improvement in performance metrics, with our improved model achieving a 2.5% increase in mean average precision compared to the original YOLO v7. Furthermore, our approach attained a 94.2% accuracy in CCSB fitting and a 91.3% Chinese cabbage identification rate. Compared to traditional methods such as the Hough transform and linear regression, our method showed an 18.6% increase in the CCSB identification rate and a 17.6% improvement in angle accuracy. The novelty of this study lies in the innovative combination of the YOLO v7 model with a decoupled head and the BiFormer attention mechanism, which together advance the identification and fitting of overlapping leafy vegetables. This advancement supports intelligent weeding, reduces the reliance on chemical herbicides, and promotes safer, more sustainable agricultural practices. Our research not only improves the accuracy of overlapping vegetable identification, but also provides a robust framework for enhancing precision agriculture.

Thymol Deploys Multiple Antioxidative Systems to Suppress ROS Accumulation in Chinese Cabbage Seedlings under Saline Stress

Developing biostimulants is a promising approach for sustainable agriculture under a saline environment. Thymol is a plant-derived compound with a potential antioxidative capacity. However, little is known about whether and how the antioxidative property of thymol plays a role in inducing plant tolerance against abiotic stresses. Here, we find that thymol induces saline tolerance in Chinese cabbage seedlings via enhancing the antioxidative capacity. Treatment with NaCl (100 mM) decreased the seedling fresh weight by 59.9% as compared to a control. Thymol at 20 μM showed the greatest effect on promoting seedling growth under saline stress, with the seedling fresh weight being increased by 71.0% as compared to NaCl treatment. Thymol remarkably decreased the overaccumulation of ROS (hydrogen peroxide and a superoxide radical); cell membrane damage (evaluated by lipid oxidation, membrane integrity, and relative conductivity); and cell death in seedlings under saline stress. Thymol induced three antioxidative systems to lower the ROS level in salt-treated seedlings. First, thymol remarkably activated a set of antioxidative enzymes, such as SOD (superoxide dismutase), APX (ascorbate peroxidase), CAT (catalase), and POD (peroxidase). Second, thymol balanced the cellular redox status by increasing the ratio of AsA/DHA (ascorbic acid/dehydroascorbic acid) and GSH/GSSG (glutathione/oxidized glutathione). Third, thymol significantly enhanced the level-two kinds of antioxidants (total phenol and flavonoid). All of these physiological responses were observed in both the shoots and the roots. In sum, thymol deploys multiple antioxidative systems to help Chinese cabbage seedlings against saline stress. Such findings suggest that thymol has great potential to be developed as a novel biostimulant enhancing crop tolerance against saline stress.

Promoting effect of low concentration strontium on photosynthetic performance of Chinese cabbage seedlings: Combined leaf characteristics, photosynthetic carbon assimilation and chlorophyll fluorescence

Low concentration strontium (LC-Sr) can promote the growth of plants. In order to explore its promoting mechanism from the aspect of photosynthesis, the leaf characteristics, CO2 assimilation and chlorophyll (Chl) a fluorescence kinetics were investigated with hydroponically LC-Sr-treated Chinese cabbage seedlings. After a 28-d treatment to SrCl2 at different concentrations (0.1, 0.2, 0.5, and 1.0 mmol L−1), we observed an increase in the specific leaf weight (SLW) of Chinese cabbage compared with the control group. Notably, as the strontium concentration increased, a more pronounced improvement trend in the contents of Chl and protein in the leaves was observed, contributing to the enhancement of photosynthesis. However, the statistical differences in Pn among various LC-Sr treatments were not significant. Nevertheless, the leaf starch content exhibited a significant increase after LC-Sr treatments. Additionally, Chl a fluorescence transient has been used as a sensitive indicator of the promotional effect of LC-Sr on photosynthesis. The results of fluorescence parameters showed that LC-Sr treatments accelerated the light reaction speed of leaves (Tfm, dV/dto, dVG/dto), improved the energy utilization efficiency of photosystem (PSI and PSII) (ETo/CSo, ψET,ψRE, δRo, φRo), and ultimately enhanced the photosynthetic performance of leaves (PIabs, SFIabs, DFabs). The increased RCs/CSo and Sm contributed to the enhancement of the light reaction activity of strontium-treated leaves. The LC-Sr treatments had no interference with the calcium absorption, and notably enhanced the photosynthetic capacity of Chinese cabbage, shedding light on potential benefits of LC-Sr for crop cultivation.

Elaboration of a green and highly dispersible Co-MOFs induced Co-doped C3N5 photocatalyst: Applications and mechanistic insights into activated perxymonosulfate degradation of antibiotics

In recent years, metal-organic frameworks (MOFs) as metal-based carbon materials have been widely concerned with the application of activated perxymonosulfate(PMS) to degrade antibiotics. However, constructing MOFs-derived materials with excellent dispersion, highly exposed metal active sites, and environmental friendliness remains a challenge. This paper reports for the first time the in-situ formation of nitrogen-rich Co-doped C3N5 (Co-C3N5) materials using a cobalt-based metal-organic framework (Co-MOFs), which has highly dispersed metal sites that are conducive to the activation of PMS. The forbidden bandwidth value of Co-C3N5 photocatalyst is as low as ∼1.20 eV, which could completely degrade chlortetracycline hydrochloride (CTC) with the initial concentration of 30 mg/L within 6 min under a light source of 50 W LED lamp. The results indicate that efficient photoelectron separation and transport are key factors in the activation and degradation of CTC by PMS, involving both free radical and non-free radical degradation processes. Meanwhile, the skeletal structure of MOFs and the triazine ring of C3N5 were retained, enhancing the stability of the photocatalyst and reducing the leaching of cobalt Finally, the seed germination experiment of Chinese cabbage showed that the treated CTC could increase the root length of Chinese cabbage seedlings by 10.1∼42.9%, which proved that the materials had no toxic effect on environment and could provide carbon and nitrogen source for plant growth. Based on this, this study proposes a novel approach for the development of MOFs-derived carbon photocatalysts that exhibit excellent dispersion and highly exposed metal active sites.

Drought Tolerance Evaluation and Growth Response of Chinese Cabbage Seedlings to Water Deficit Treatment

Drought is a significant climatic factor that significantly affects the production of Chinese cabbage, a crop that is highly susceptible to drought stress. The development and cultivation of drought-tolerant varieties could be a viable strategy to minimize the damage caused by climate change and ensure stable production of Chinese cabbage. This requires the implementation of technologies for early evaluation and selection of a plethora of resources. In this study, we screened 100 varieties and breeding resources for drought tolerance under a water deficit treatment at the seedling stage. We also evaluated the growth response of Chinese cabbage varieties and breeding resources under water deficit treatment and selected drought-tolerant Chinese cabbage genotypes. We confirmed that the visual score for wilting, which evaluates the wilting response during the recovery process of Chinese cabbage seedlings through water deficit treatment and re-watering, can be used as an indicator for evaluating tolerance to drought stress. The visual score for wilting showed a high correlation with major traits representing drought tolerance. Our findings highlight the need for an integrated approach that considers various environmental conditions, varieties, and lines to select and develop drought-tolerant varieties. We selected ‘18-FH112-1’ and ‘18-FH112-1-2’ among others, and these germplasms will be useful resources for drought tolerance breeding. This study provides a foundation for future efforts to develop drought-tolerant Chinese cabbage varieties, thereby contributing to the stable production of this crucial crop.

Bacteriophage cocktail for biocontrol of soft rot disease caused by Pectobacterium species in Chinese cabbage.

Pectobacterium spp. are necrotrophic plant pathogens that cause the soft rot disease in Chinese cabbage, resulting in severe yield loss. The use of conventional antimicrobial agents, copper-based bactericides, and antibiotics has encountered several limitations, such as bioaccumulation on plants and microbial resistance. Bacteriophages (phages) are considered promising alternative antimicrobial agents against diverse phytopathogens. In this study, we isolated and characterized two virulent phages (phiPccP-2 and phiPccP-3) to develop a phage cocktail. Morphological and genomic analyses revealed that two phages belonged to the Tevenvirinae and Mccorquodalevirinae subfamilies, respectively. The phiPccP-2 and phiPccP-3 phages, which have a broad host range, were stable at various environmental conditions, such as various pHs and temperatures and exposure to ultraviolet light. The phage cocktail developed using these two lytic phages inhibited the emergence of phage-resistant bacteria compared to single-phage treatments in in vitro challenge assays. The phage cocktail treatment effectively prevented the development of soft rot symptom in matured Chinese cabbage leaves. Additionally, the phage cocktail comprising three phages (phiPccP-1, phiPccP-2, and phiPccP-3) showed superior biocontrol efficacy against the mixture of Pectobacterium strains in Chinese cabbage seedlings. These results suggest that developing phage cocktails is an effective approach for biocontrol of soft rot disease caused by Pectobacterium strains in crops compared to single-phage treatments. KEY POINTS: •Two newly isolated Pectobacterium phages, phiPccP-2 and phiPccP-3, infected diverse Pectobacterium species and effectively inhibited the emergence of phage-resistant bacteria. •Genomic and physiological analyses suggested that both phiPccP-2 and phiPccP-3 are lytic phages and that their lytic activities are stable in the environmental conditions under which Chinese cabbage grows. •Treatment using a phage cocktail comprising phiPccP-2 and phiPccP-3 efficiently suppressed soft rot disease in detached mature leaves and seedlings of Chinese cabbage, indicating the applicability of the phage cocktail as an alternative antimicrobial agent.

Green hydrothermal synthesis of multifunctional carbon dots from cassava pulps for metal sensing, antioxidant, and mercury detoxification in plants

Carbon dots (CDs) have been attracted to nanocarbon materials for metal ion sensing, biological activity, and plant phytotoxicity due to their excellent photophysical properties, such as low cytotoxicity, high quantum yield, tunable fluorescence emission, and biocompatibility. Cassava pulp, which consists mainly of starch, has been identified as a low-cost biomass waste from the cassava starch industry. Therefore, this research developed CDs and nitrogen-doped CDs (NCDs) from cassava pulp using a one-step hydrothermal process in deionized water at 200 °C. The effects of the synthesis conditions, including reaction time (6–24 h) and the nitrogen doping derivatives, were also investigated. CDs and ethylenediamine doped-NCDs exhibited tunable fluorescence emission, strong quantum yield, high photostability, and tolerance to photobleaching. Furthermore, the potential applications of CDs-12 h were demonstrated such as fluorescent sensors for metal ion sensing, antioxidant activity, and mercury detoxification in plants. Fluorescence quenching of the CDs-12 h via both static and dynamic quenching mechanisms was observed in the presence of several metal ions such as Hg2+, Cu2+, and Fe3+ with the detection limit in micromolar levels and further applied to real water samples with good recovery and acceptable relative standard derivation. The paper test strip coated with CDs-12 h could also detect these metal ions under UV light. CDs and NCDs-EDA also showed potential DPPH radical scavenging activity and alleviated mercury toxicity in the Chinese cabbage seedlings with the incubation of CDs-12 h and NCDs-EDA-12 h (30 mg/L).

Prediction of Growth and Quality of Chinese Cabbage Seedlings Cultivated in Different Plug Cell Sizes via Analysis of Image Data Using Multispectral Camera

In recent times, there has been an increasing demand for the development of rapid and non-destructive assessment of the growth and quality of seedlings before transplanting. This study was conducted to examine the growth and quality of Chinese cabbage seedlings that can be determined via the image data acquired using a multispectral camera. Chinese cabbage seedlings were cultivated in five different plug trays (72, 105, 128, 162, and 200 cells/tray) for 30 days after sowing (DAS). The growth of seedlings had no significant difference in the early stage of cultivation; however, it decreased with increasing the number of cells in the plug tray due to the restricted root zone volume in the mid to late stages. Individual leaf area was predicted by analyzing of image data with high accuracy (R2 > 0.8) after 15 DAS; however, the accuracy of leaf area prediction per tray decreased due to overlapping and twisting leaves. Among six different vegetation indices, mrNDVI showed a high correlation (R2 > 0.6) with the dry weight of seedlings at 25 and 30 DAS. We confirmed that the leaf area of seedlings can be predicted non-destructively by analyzing the acquired image data per seedling and tray and suggested the applicability of vegetation indices for predicting the growth and quality of vegetable seedlings.

Fulvic Acid Alleviates the Toxicity Induced by Calcium Nitrate Stress by Regulating Antioxidant and Photosynthetic Capacities and Nitrate Accumulation in Chinese Flowering Cabbage Seedlings

Continuous cropping can lead to an excessive accumulation of nitrate in facility-cultured soil. Excessive accumulation of nitrate gradually becomes the main reason for crop failure in vegetables and endangers human health. Therefore, the exploration of effective measures to decrease abundant nitrate accumulation in Chinese flowering cabbage is indispensable. In this study, a kind of plant growth regulator, fulvic acid (FA), was used to study its positive effect on alleviating the growth inhibition induced by excessive Ca(NO3)2 in Chinese flowering cabbage. Meanwhile, we conducted hydroponic cultivation and measured the growth indices, photosynthetic and oxidation-reduction characteristics of Chinese flowering cabbage with different treatments. After determining the optimal treatment concentration, we mainly designed four treatment groups, including Con, FA, Ca(NO3)2 and FA + Ca(NO3)2 cotreatment, to explore the regulatory mechanism by which FA alleviates Ca(NO3)2 stress in Chinese flowering cabbage. The results showed that FA can effectively alleviate the inhibitory effect of excessive Ca(NO3)2 on the growth of Chinese flowering cabbage seedlings. FA recovered the photosynthetic capacity of seedlings under Ca(NO3)2 stress. In addition, FA depressed the accumulation of O2·−, H2O2, malondialdehyde (MDA) and relative electrical conductivity, but increased the activity of antioxidant enzymes, including SOD, POD, CAT and APX, which finally enhanced the stress resistance of Chinese flowering cabbage to Ca(NO3)2. The expression of nitrate-related transporters, BcNRT1.1 and BcNRT1.5, was depressed by FA, which inhibited redundant nitrate absorption and restricted more nitrate from being stored in the roots instead of being transferred to the shoot. Ultimately, nitrate accumulation in the edible part was reduced in Chinese flowering cabbage seedlings. In general, exogenous FA may alleviate nitrate stress by improving oxidation resistance, photosynthetic capacity and redundant Ca(NO3)2 accumulation in Chinese flowering cabbage.

Fast, simple, efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots

Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However, the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated (ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches (one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings (Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%–51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu Caixin. The one-step method outperformed the two-step method; the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily, quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding.

Genome-Wide Identification of BrCAX Genes and Functional Analysis of BrCAX1 Involved in Ca2+ Transport and Ca2+ Deficiency-Induced Tip-Burn in Chinese Cabbage (Brassica rapa L. ssp. pekinensis).

Calcium (Ca2+) plays essential roles in plant growth and development. Ca2+ deficiency causes a physiological disorder of tip-burn in Brassiceae crops and is involved in the regulation of cellular Ca2+ homeostasis. Although the functions of Ca2+/H+ exchanger antiporters (CAXs) in mediating transmembrane transport of Ca2+ have been extensively characterized in multiple plant species, the potential roles of BrCAX genes remain unclear in Chinese cabbage. In this study, eight genes of the BrCAX family were genome-widely identified in Chinese cabbage. These BrCAX proteins contained conserved Na_Ca_ex domain and belonged to five members of the CAX family. Molecular evolutionary analysis and sequence alignment revealed the evolutionary conservation of BrCAX family genes. Expression profiling demonstrated that eight BrCAX genes exhibited differential expression in different tissues and under heat stress. Furthermore, Ca2+ deficiency treatment induced the typical symptoms of tip-burn in Chinese cabbage seedlings and a significant decrease in total Ca2+ content in both roots and leaves. The expression changes in BrCAX genes were related to the response to Ca2+ deficiency-induced tip-burn of Chinese cabbage. Specially, BrCAX1-1 and BrCAX1-2 genes were highly expressed gene members of the BrCAX family in the leaves and were significantly differentially expressed under Ca2+ deficiency stress. Moreover, overexpression of BrCAX1-1 and BrCAX1-2 genes in yeast and Chinese cabbage cotyledons exhibited a higher Ca2+ tolerance, indicating the Ca2+ transport capacity of BrCAX1-1 and BrCAX1-2. In addition, suppression expression of BrCAX1-1 and BrCAX1-2 genes reduced cytosolic Ca2+ levels in the root tips of Chinese cabbage. These results provide references for functional studies of BrCAX genes and to investigate the regulatory mechanisms underlying Ca2+ deficiency disorder in Brassiceae vegetables.

Identification of heat tolerance and screening of heat tolerance indexes in different Chinese cabbage seedlings

Under the trends of global warming, the high and stable yield of Chinese cabbage has been affected by high temperature and heat damage. Establishing a simple, convenient, effective, and replicable heat tolerance evaluation model for Chinese cabbage is the key to screening and innovating of heat tolerance germplasm resources and to developing new Chinese cabbage varieties. In this experiment, 31 Chinese cabbage varieties were used as experimental materials. By experimentation with normal and artificially high (40 °C) temperatures, 12 seedling physiological indexes were determined. Based on the heat resistance coefficient (y) of every index, the heat resistance of different fast-growing Chinese cabbage varieties was comprehensively evaluated with multivariate analysis. The results showed that high-temperature stress had different effects on each index, and that the correlation between candidate indexes exhibited information overlap and a cross phenomenon. Principal component analysis transformed 12 single indexes of Chinese cabbage seedlings under high-temperature stress into 2 independent comprehensive indexes and generated the comprehensive evaluation value (D value) of heat tolerance for different varieties. On this basis, the evaluation equation for heat resistance in Chinese cabbage was established with the stepwise regression method. Cluster analysis enabled the classification of 31 Chinese cabbage varieties into four categories: extremely heat-resistant, moderately heat resistant, non-heat resistant heat intolerant. Extremely heat-resistant varieties and extremely heat intolerant varieties can serve as materials for the breeding of heat-resistant varieties and for the study of the heat-resistance and regulatory mechanisms of Chinese cabbage. chlorophyll a, malondialdehyde, and stomatal conductance can be used as evaluation indexes of the heat tolerance in Chinese cabbage at the seedling stage. Finally, six commercial Chinese cabbage varieties were used to verify the model. The verification results show that the model is accurate and reliable and can be used in production practice.

Unlocking the potential of humic acid production through oxygen-assisted hydrothermal humification of hydrochar

The utilization of biomass as a raw material and production of hydrothermal humic acid (HHA) through hydrothermal method using hydrochar as an intermediate product is a sustainable approach for the preparation of humic acid. In this study, hydrochar was prepared through acid hydrothermal treatment of corn straw and then oxygen was introduced to alkaline hydrothermal treatment of hydrochar to improve the yield of humic acid. It was discovered that an oxygen pressure of 5 bar could significantly enhance the yield of HHA from 41.9 wt% to 49.2 wt% at a hydrothermal temperature of 180 °C, while oxygen pressures of 10 bar and 20 bar led to a decrease in HHA yield (41.5 wt% to 28.2 wt%). Oxygen addition could also reduce the energy input and the economic cost required for hydrothermal reactions. Further study revealed that the increase in HHA yield was the oxidation of aliphatic carbon in the solid residue derived from hydrochar by oxygen, which became part of the HHA and resulted in weaker fluorescence intensity of the HHA. However, excessive oxygen could increase the oxygen-containing functional groups of the HHA while also causing further cleavage of macromolecular components with high carbon numbers, generating smaller molecules that partially dissolve in the liquid phase. The HHA synthesized at an oxygen pressure of 5 bar showed good performance in promoting Chinese cabbage seedlings growth and was superior to the HHA synthesized in the absence of oxygen in terms of cadmium adsorption. This study proved an improved hydrothermal humification method and provided technical support for industrial applications.

Supramolecular Fluorescence Sensor Array Based on Cucurbit[8]uril Complexes Used for the Detection of Multiplex Quaternary Ammonium Pesticides.

The simultaneous detection of multiple quaternary ammonium pesticides (QAPs) in water is a challenge due to their high solubility in water and similar structures. In this paper, we have developed a quadruple-channel supramolecular fluorescence sensor array for the simultaneous analysis of five QAPs, including paraquat (PQ), diquat (DQ), difenzoquat (DFQ), mepiquat (MQ), and chlormequat (CQ). Not only were QAP samples of different concentrations (10, 50, and 300 μM) in water distinguished with 100% accuracy but also single QAP and binary QAP mixed samples (DFQ-DQ) were sensitively quantified. Our experimental interference study confirmed that the developed array has good anti-interference ability. The array can quickly identify five QAPs in river and tap water samples. In addition, it also qualitatively detected QAP residues in Chinese cabbage and wheat seedlings extract. This array has rich output signals, low cost, easy preparation, and simple technology, demonstrating great potential in environmental analysis.

Anti-oviposition and repellence of Cordyceps fumosorosea against Spodoptera exigua

Anti-oviposition induced by Cordyceps fumosorosea FG340 to Spodoptera exigua and its persistence had been assessed on the Chinese cabbage seedlings sprayed with 0, 106, 107 and 108 conidia per mL in greenhouse and laboratory. In a randomized complete block, four couples (males and females) of moths were released in each cage. In the laboratory, the percentage of egg laid varied significantly between treatments with 59.8, 14.8, 11.9, and 13.5% on 0, 106, 107 and 108 conidia per mL; respectively. Similarly, in the greenhouse, the percentage of eggs laid differed significantly between suspensions with 88.18, 8.7, 0, and 3.13% on 0, 106, 107, and 108 conidia per mL; respectively. Repellence study showed that there is a major difference between suspensions with average 34.55, 35.21 and 40.02% for 106, 107 and 108 conidia per mL; respectively. Endophytic C. fumosorosea and its culture fluid affect the behavior of armyworm larvae. The concentration of 108 conidia per mL can affect the oviposition of the armyworm.

Application of UPLC-Orbitrap-HRMS targeted metabolomics in screening of allelochemicals and model plants of ginseng

Continuous cropping of ginseng leads to serious declines in yield and quality because of self-toxicity of allelochemicals and other factors in soil. However, because of the long growth cycle and low survival rate of ginseng, rapid screening of autotoxic activity is difficult. Therefore, it is important to analyze the allelochemicals and identify a model plant with autotoxic responses similar to those of ginseng. In this study, UPLC-Orbitrap-HRMS targeted metabolomics and verification of autotoxic activity were used to analyze a problem soil from continuously cropped ginseng. Allelochemical markers were screened by OPLS-DA. Seeds and seedlings of maize, Chinese cabbage, cucumber, green beans, wheat, sunflower, and oats were selected to identify potential model plants. Model plants with autotoxic responses similar to those of ginseng were evaluated by comparing morphological, physiological, and biochemical characteristics. The n-butanol extract of the continuously cropped problem soil had the most significant autotoxic activity. Twenty-three ginsenosides and the contributions to autotoxic effects were screened and evaluated. Of potential model plants, seeds and seedlings of cucumber showed similar growth inhibition to that of ginseng under the action of allelochemicals. Thus, metabolomics can be used to screen allelochemicals in soil and predict the autotoxic effects, and the cucumber plant model can be used to rapidly screen allelopathic activity of ginseng. The study will provide reference for methodology in allelopathy research on ginseng.