China National Center For Bioinformation Research Articles

Plant genomic resources at National Genomics Data Center: assisting in data-driven breeding applications.

The online version contains supplementary material available at 10.1007/s42994-023-00134-4.

Database Resources of the National Genomics Data Center, China National Center for Bioinformation in 2024.

The National Genomics Data Center (NGDC), which is a part of the China National Center for Bioinformation (CNCB), provides a family of database resources to support the global academic and industrial communities. With the rapid accumulation of multi-omics data at an unprecedented pace, CNCB-NGDC continuously expands and updates core database resources through big data archiving, integrative analysis and value-added curation. Importantly, NGDC collaborates closely with major international databases and initiatives to ensure seamless data exchange and interoperability. Over the past year, significant efforts have been dedicated to integrating diverse omics data, synthesizing expanding knowledge, developing new resources, and upgrading major existing resources. Particularly, several database resources are newly developed for the biodiversity of protists (P10K), bacteria (NTM-DB, MPA) as well as plant (PPGR, SoyOmics, PlantPan) and disease/trait association (CROST, HervD Atlas, HALL, MACdb, BioKA, BioKA, RePoS, PGG.SV, NAFLDkb). All the resources and services are publicly accessible at https://ngdc.cncb.ac.cn.

Five improved sesame reference genomes and genome resequencing unveil the contribution of structural variants to genetic diversity and yield-related traits variation.

Five improved sesame reference genomes and genome resequencing unveil the contribution of structural variants to genetic diversity and yield-related traits variation.

The Populus koreana genome provides insights into the biosynthesis of plant aroma

Populus koreana is a tree species that shows unique fragrance traits because of the emission of volatiles from tissues, thus it has great potential for the study of aroma in forest trees. The genome of P. koreana, with a size of 429.47 Mb and N50 contigs of 4.97 Mb, was assembled into 19 chromosomes and 38,075 genes were predicted. P. koreana and Populus trichocarpa diverged from a common ancestor before 6.14 million years ago (Mya). Metabolome results suggested that the main aroma components terpenes and esters are significantly enriched in P. koreana leaves. Compared with that of P. trichocarpa, the P. koreana genome contains many more terpene synthase genes (TPSs), which encode proteins enriched in terpenoid-related metabolic pathways associated with the production of plant volatiles. Among these TPS genes, TPS-a/b might largely contribute to the synthesis of abundant sesquiterpenes and monoterpenes. Our results provide a valuable genomic resource for the further study of the production of aromatic volatiles by forest trees. Gene families related to terpenoid biosynthesis are substantially expanded in P. koreana, mainly resulting from tandem duplications and lineage specific whole-genome duplications. The evidence presented in this study increases our understanding of the evolution and diversity of plant volatile compounds, which could accelerate the selective breeding on traits of interest in Populus. Data availability statementThe datasets of transcriptome and genome were uploaded in NCBI and China national center for bioinformation, respectively. The accession numbers were PRJCA012303, CRA012924, and PRJNA886606 (NCBI).

Early Diagnosis of COVID-19 Images Using Optimal CNN Hyperparameters

Coronavirus disease (COVID-19) is a worldwide epidemic that poses substantial health hazards. However, COVID-19 diagnostic test sensitivity is still restricted due to abnormalities in specimen processing. Meanwhile, optimizing the highly defined number of convolutional neural network (CNN) hyperparameters (hundreds to thousands) is a useful direction to improve its overall performance and overcome its cons. Hence, this paper proposes an optimization strategy for obtaining the optimal learning rate and momentum of a CNN's hyperparameters using the grid search method to improve the network performance. Therefore, three alternative CNN architectures (GoogleNet, VGG16, and ResNet) were used to optimize hyperparameters utilizing two different COVID-19 radiography data sets (Kaggle (X-ray) and China national center for bio-information (CT)). These architectures were tested with/without optimizing the hyperparameters. The results confirm effective disease classification using the CNN structures with optimized hyperparameters. Experimental findings indicate that the new technique outperformed the previous in terms of accuracy, sensitivity, specificity, recall, F-score, false positive and negative rates, and error rate. At epoch 25, the optimized Resnet obtained high classification accuracy, reaching 98.98% for X-ray images and 98.78% for CT images.

Database Resources of the National Genomics Data Center, China National Center for Bioinformation in 2023.

The National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB), provides a family of database resources to support global academic and industrial communities. With the explosive accumulation of multi-omics data generated at an unprecedented rate, CNCB-NGDC constantly expands and updates core database resources by big data archive, integrative analysis and value-added curation. In the past year, efforts have been devoted to integrating multiple omics data, synthesizing the growing knowledge, developing new resources and upgrading a set of major resources. Particularly, several database resources are newly developed for infectious diseases and microbiology (MPoxVR, KGCoV, ProPan), cancer-trait association (ASCancer Atlas, TWAS Atlas, Brain Catalog, CCAS) as well as tropical plants (TCOD). Importantly, given the global health threat caused by monkeypox virus and SARS-CoV-2, CNCB-NGDC has newly constructed the monkeypox virus resource, along with frequent updates of SARS-CoV-2 genome sequences, variants as well as haplotypes. All the resources and services are publicly accessible at https://ngdc.cncb.ac.cn.

EHypertension: A prospective longitudinal multi‐omics essential hypertension cohort

eHypertension: A prospective longitudinal multi‐omics essential hypertension cohort

COVID-CT-Mask-Net: prediction of COVID-19 from CT scans using regional features.

We present COVID-CT-Mask-Net model that predicts COVID-19 in chest CT scans. The model works in two stages: in the first stage, Mask R-CNN is trained to localize and detect two types of lesions in images. In the second stage, these detections are fused to classify the whole input image. To develop the solution for the three-class problem (COVID-19, Common Pneumonia and Control), we used the COVIDx-CT data split derived from the dataset of chest CT scans collected by China National Center for Bioinformation. We use 3000 images (about 5% of the train split of COVIDx-CT) to train the model. Without any complicated data normalization, balancing and regularization, and training only a small fraction of the model’s parameters, we achieve a 90.80% COVID-19 sensitivity, 91.62% Common Pneumonia sensitivity and 92.10% true negative rate (Control sensitivity), an overall accuracy of 91.66% and F1-score of 91.50% on the test data split with 21192 images, bringing the ratio of test to train data to 7.06. We also establish an important result that regional predictions (bounding boxes with confidence scores) detected by Mask R-CNN can be used to classify whole images. The full source code, models and pretrained weights are available on https://github.com/AlexTS1980/COVID-CT-Mask-Net.

Database Resources of the National Genomics Data Center, China National Center for Bioinformation in 2022.

The National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB), provides a family of database resources to support global research in both academia and industry. With the explosively accumulated multi-omics data at ever-faster rates, CNCB-NGDC is constantly scaling up and updating its core database resources through big data archive, curation, integration and analysis. In the past year, efforts have been made to synthesize the growing data and knowledge, particularly in single-cell omics and precision medicine research, and a series of resources have been newly developed, updated and enhanced. Moreover, CNCB-NGDC has continued to daily update SARS-CoV-2 genome sequences, variants, haplotypes and literature. Particularly, OpenLB, an open library of bioscience, has been established by providing easy and open access to a substantial number of abstract texts from PubMed, bioRxiv and medRxiv. In addition, Database Commons is significantly updated by cataloguing a full list of global databases, and BLAST tools are newly deployed to provide online sequence search services. All these resources along with their services are publicly accessible at https://ngdc.cncb.ac.cn.

2019nCoVR—A comprehensive genomic resource for SARS-CoV-2 variant surveillance

The coronavirus disease 2019 (COVID-19) is a once-in-a-century pandemic, and the virus, the severe acute respiratory syndrome coronavirus 2 or SARS-CoV-2, has infected more than 180 million people and claimed almost 4 million lives worldwide (as of July 5, 2021) since the first case was reported in Wuhan, China, on December 31, 2019. The China National Center for Bioinformation (CNCB) responded promptly and launched the 2019 Novel Coronavirus Resource (2019nCoVR; https://ngdc.cncb.ac.cn/ncov/) in January 2020 for rapid release and public sharing of SARS-CoV-2 genomic data and analysis tools (Figure 1A).

Delta.AR: An augmented reality-based visualization platform for 3D genome

Delta.AR: An augmented reality-based visualization platform for 3D genome

Genome Warehouse: A Public Repository Housing Genome-scale Data

The Genome Warehouse (GWH) is a public repository housing genome assembly data for a wide range of species and delivering a series of web services for genome data submission, storage, release, and sharing. As one of the core resources in the National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB; https://ngdc.cncb.ac.cn), GWH accepts both full and partial (chloroplast, mitochondrion, and plasmid) genome sequences with different assembly levels, as well as an update of existing genome assemblies. For each assembly, GWH collects detailed genome-related metadata of biological project, biological sample, and genome assembly, in addition to genome sequence and annotation. To archive high-quality genome sequences and annotations, GWH is equipped with a uniform and standardized procedure for quality control. Besides basic browse and search functionalities, all released genome sequences and annotations can be visualized with JBrowse. By May 21, 2021, GWH has received 19,124 direct submissions covering a diversity of 1108 species and has released 8772 of them. Collectively, GWH serves as an important resource for genome-scale data management and provides free and publicly accessible data to support research activities throughout the world. GWH is publicly accessible at https://ngdc.cncb.ac.cn/gwh.

COVIDNet-CT: A Tailored Deep Convolutional Neural Network Design for Detection of COVID-19 Cases From Chest CT Images.

The coronavirus disease 2019 (COVID-19) pandemic continues to have a tremendous impact on patients and healthcare systems around the world. In the fight against this novel disease, there is a pressing need for rapid and effective screening tools to identify patients infected with COVID-19, and to this end CT imaging has been proposed as one of the key screening methods which may be used as a complement to RT-PCR testing, particularly in situations where patients undergo routine CT scans for non-COVID-19 related reasons, patients have worsening respiratory status or developing complications that require expedited care, or patients are suspected to be COVID-19-positive but have negative RT-PCR test results. Early studies on CT-based screening have reported abnormalities in chest CT images which are characteristic of COVID-19 infection, but these abnormalities may be difficult to distinguish from abnormalities caused by other lung conditions. Motivated by this, in this study we introduce COVIDNet-CT, a deep convolutional neural network architecture that is tailored for detection of COVID-19 cases from chest CT images via a machine-driven design exploration approach. Additionally, we introduce COVIDx-CT, a benchmark CT image dataset derived from CT imaging data collected by the China National Center for Bioinformation comprising 104,009 images across 1,489 patient cases. Furthermore, in the interest of reliability and transparency, we leverage an explainability-driven performance validation strategy to investigate the decision-making behavior of COVIDNet-CT, and in doing so ensure that COVIDNet-CT makes predictions based on relevant indicators in CT images. Both COVIDNet-CT and the COVIDx-CT dataset are available to the general public in an open-source and open access manner as part of the COVID-Net initiative. While COVIDNet-CT is not yet a production-ready screening solution, we hope that releasing the model and dataset will encourage researchers, clinicians, and citizen data scientists alike to leverage and build upon them.

The Global Landscape of SARS-CoV-2 Genomes, Variants, and Haplotypes in 2019nCoVR

On January 22, 2020, China National Center for Bioinformation (CNCB) released the 2019 Novel Coronavirus Resource (2019nCoVR), an open-access information resource for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). 2019nCoVR features a comprehensive integration of sequence and clinical information for all publicly available SARS-CoV-2 isolates, which are manually curated with value-added annotations and quality evaluated by an automated in-house pipeline. Of particular note, 2019nCoVR offers systematic analyses to generate a dynamic landscape of SARS-CoV-2 genomic variations at a global scale. It provides all identified variants and their detailed statistics for each virus isolate, and congregates the quality score, functional annotation, and population frequency for each variant. Spatiotemporal change for each variant can be visualized and historical viral haplotype network maps for the course of the outbreak are also generated based on all complete and high-quality genomes available. Moreover, 2019nCoVR provides a full collection of SARS-CoV-2 relevant literature on the coronavirus disease 2019 (COVID-19), including published papers from PubMed as well as preprints from services such as bioRxiv and medRxiv through Europe PMC. Furthermore, by linking with relevant databases in CNCB, 2019nCoVR offers data submission services for raw sequence reads and assembled genomes, and data sharing with NCBI. Collectively, SARS-CoV-2 is updated daily to collect the latest information on genome sequences, variants, haplotypes, and literature for a timely reflection, making 2019nCoVR a valuable resource for the global research community. 2019nCoVR is accessible at https://bigd.big.ac.cn/ncov/.

DatabaseResources of the National Genomics Data Center, China National Center for Bioinformation in 2021.

The National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB), provides a suite of database resources to support worldwide research activities in both academia and industry. With the explosive growth of multi-omics data, CNCB-NGDC is continually expanding, updating and enriching its core database resources through big data deposition, integration and translation. In the past year, considerable efforts have been devoted to 2019nCoVR, a newly established resource providing a global landscape of SARS-CoV-2 genomic sequences, variants, and haplotypes, as well as Aging Atlas, BrainBase, GTDB (Glycosyltransferases Database), LncExpDB, and TransCirc (Translation potential for circular RNAs). Meanwhile, a series of resources have been updated and improved, including BioProject, BioSample, GWH (Genome Warehouse), GVM (Genome Variation Map), GEN (Gene Expression Nebulas) as well as several biodiversity and plant resources. Particularly, BIG Search, a scalable, one-stop, cross-database search engine, has been significantly updated by providing easy access to a large number of internal and external biological resources from CNCB-NGDC, our partners, EBI and NCBI. All of these resources along with their services are publicly accessible at https://bigd.big.ac.cn.