Chilling Requirement Research Articles

Chilling and Forcing Requirements of Wintersweet (Chimonanthus praecox L.) Flowering in China

Numerous studies have reported phenological changes and their driving mechanisms in spring flowering plants. However, there is little research on the shifts of winter flowering phenology and its response to forcing and chilling requirements. Based on the China Phenological Observation Network (CPON) ground observation data from nine sites over the past 20 years, we explored the spatial and temporal variation patterns of flowering plants and their response to chilling and forcing in wintersweet (Chimonanthus praecox L.), a common winter flowering plant species in temperate and subtropical zones of China. We used three chilling models (chilling hour, Utah, and dynamic models) and the growing degree hours (GDHs) model to calculate each site’s daily chilling and forcing. Using the partial least squares (PLSs) regression approach, we established the relationship between the first flowering date (FFD) and pre-season chilling and forcing in wintersweet, based on which we identified chilling and forcing periods and calculated chilling and forcing requirements. This study found that the FFD of wintersweet in China showed an overall advancement trend during the last 20 years. Still, there were temporal and spatial differences in the FFD of wintersweet among different sites. The PLS results showed that wintersweet also had periods of chilling and forcing, both of which co-regulated wintersweet flowering. We found the forcing and chilling requirements of wintersweet varied significantly from site to site. The higher the latitude is, the more chilling requirements are needed. The chilling requirements for wintersweet were about 6.9–34.9 Chill Portions (CPs) and 1.4–21.6 CP in the temperate and subtropical zones, respectively, with corresponding forcing requirements of 3.2–1922.9 GDH and 965.3–8482.6 GDH, respectively. In addition, we found that the temperature requirements of wintersweet were correlated by a negative exponential relationship, suggesting that chilling and forcing requirements have an antagonistic effect on initiating flowering phenology. Our results could help us understand how flowering dates of winter flowering plants respond to climate change.

Alternative splicing and deletion in S-RNase confer stylar-part self-compatibility in the apple cultivar 'Vered'.

Although self-incompatibility in apples (Malus × domestica Borkh.) is regulated by a single S-locus with multiple S-haplotypes that comprise pistil S (S-RNase) and pollen S genes, it is not desirable in commercial orchards because it requires cross-pollination to achieve stable fruit production. Therefore, it is important to identify and characterize self-compatible apple cultivars. However, little is known about self-compatibility(SC) and its underlying molecular mechanisms in apples. In this study, we discovered that 'Vered', an early maturing and low chilling-requiring apple cultivar, exhibits stable SC, which was evaluated via self-pollination tests. The S-genotype of 'Vered' was designated as S24S39sm. Results of genetic analysis of selfed progeny of 'Vered' revealed that SC is associated with the S39sm-haplotype, and molecular analyses indicated that it is caused by alternative splicing and a 205-bp deletion in S39sm-RNase. These events induce frameshifts and ultimately produce the defective S39sm-RNase isoforms that lack their C-terminal half. These results enabled us to develop a 117-bp DNA marker that can be used to assist in the selection of self-compatible apples with the dysfunctional S39sm-RNase. Thus, analysis of 'Vered' provided insights into the molecular mechanism of the very rare trait of natural stylar-part SC. Moreover, 'Vered' is a valuable genetic resource for breeding cultivars with SC and/or low chilling requirement in apple. Our findings contribute to a better understanding of self-compatible molecular mechanisms in apple and provide for the accelerated breeding of self-compatible apple cultivars.

Deciphering the genetic mechanisms of chilling requirement for bud endodormancy release in deciduous fruit trees.

Bud endodormancy in deciduous fruit trees is an adaptive trait evolved by selection for the capacity to survive unfavorable environmental conditions. Deciduous trees require a certain amount of winter chill named chilling requirement (CR) to promote bud endodormancy release. In recent decades, global warming has endangered the chill accumulation in deciduous fruit trees. Developing low-CR cultivars is a practical way to neutralize the effect of climate changes on the cultivation and distribution of deciduous fruit trees. In this review, we focus on the effect of chilling accumulation on bud endodormancy release and the genetic mechanisms underlying the chilling requirement in deciduous fruit trees. Additionally, we put forth a regulatory model for bud endodormancy and provide prospective directions for future research in deciduous fruit trees.

Origins and Genetic Characteristics of Egyptian Peach.

Peach (Prunus persica), a significant economic fruit tree in the Rosaceae family, is extensively cultivated in temperate and subtropical regions due to its abundant genetic diversity, robust adaptability, and high nutritional value. Originating from China over 4000 years ago, peaches were introduced to Persia through the Silk Road during the Han Dynasty and gradually spread to India, Greece, Rome, Egypt, Europe, and America. Currently grown in more than 80 countries worldwide, the expansion of peach cultivation in Egypt is mainly due to the development and utilization of peach varieties with low chilling requirements. These varieties exhibit unique phenotypic characteristics such as early maturity, reduced need for winter cold temperatures, low water requirements, and high economic value. In this study, a systematic analysis was conducted on the genetic characteristics and kinship relationships of peaches with low chilling requirements in Egypt. We conducted a comprehensive evolutionary and Identity-by-Descent (IBD) analysis on over 300 peach core germplasm resources, including Egyptian cultivars with low chilling requirements, to investigate their origin and genetic characteristics. The evolutionary analysis revealed that 'Bitter almond' is closely related to China's wild relative species Prunus tangutica Batal, while 'Early grand' shares one branch with Chinese ornamental peach cultivars, and 'Nemaguard' clusters with some ancient local varieties from China. The IBD analysis also indicated similar genetic backgrounds, suggesting a plausible origin from China. Similarly, the analysis suggested that 'Swelling' may have originated from the Czech Republic while 'Met ghamr' has connections to South Africa. 'Desert red', 'Early swelling', and 'Florida prince' are likely derived from Brazil. These findings provide valuable insights into the genetic characteristics of Egyptian peach cultivars. They offer a significant foundation for investigating the origin and spread of cultivated peaches worldwide and serve as a valuable genetic resource for breeding low chilling requirement cultivars, which is of considerable significance for the advancement of peach cultivation in Egypt.

Using harvest maturity and accumulated cold units predicts postharvest chilling requirement for ripening capacity and buttery-juicy melting texture duration in European pears

Using harvest maturity and accumulated cold units predicts postharvest chilling requirement for ripening capacity and buttery-juicy melting texture duration in European pears

Relationship between Chilling Accumulation and Heat Requirement for Flowering in Peach Varieties of Different Chilling Requirements

Relationship between Chilling Accumulation and Heat Requirement for Flowering in Peach Varieties of Different Chilling Requirements

Dormancy dynamics and cold hardiness of apple trees grown in a mild winter region

In temperate fruit plants, climate is the main factor correlating changes in plant physiology, mainly those related to entry and exit from dormancy and cold hardiness. Acclimation to cold and its relationship with dormancy are still unknown in areas with a subtropical climate. Therefore, this study aimed to investigate the dormancy dynamics and their interaction with the cold hardiness of different tissues of apple cultivars with different chilling requirements in two cultivation sites in a mild winter region in southern Brazil. The analyses were conducted from 2018 to 2022. The cultivars Gala, Fuji, and Eva were used in two environments with different altitudes. The dormancy dynamics were analyzed using the single-node cutting test, and the cold hardiness of the different tissues of each genotype was evaluated through electrolyte leakage in laboratory freezing tests. Cultivars Gala and Fuji had a greater depth of dormancy than Eva. Plants grown in orchards with higher chill accumulation had a greater depth of dormancy. Cold hardiness varied among cultivars, with the Eva cultivar having the lowest cold hardiness in mid-winter. The tissues (bud, bark, and wood) had different cold hardiness in early winter, with wood having the greatest cold hardiness, followed by bud and bark. As Eva did not go into deep dormancy, a superficial and short acclimation to cold was observed, in addition to rapid sprouting, even in winter. This condition predisposed it to cold damage to tissues in the pre-sprouting period. In this study, we found that maximum cold hardiness occurred after the end of the deep-endodormancy phase. Deacclimation of all cultivars occurred very quickly.

Effect of Warm Temperature Interruption on the Accumulation of Winter Chilling in Kiwifruit (Actinidia chinensis Planch. and A. deliciosa A. Chev.)

Warm temperature exposure during winter has reportedly resulted in the apparent negation of chilling in several fruit species. This study was conducted to investigate the floral and vegetative response of two pistillate kiwifruit cultivars to intermittent warm temperature interruption during chilling accumulation. Dormant 1-year-old canes of Actinidia chinensis ‘AU Golden Dragon’ and Actinidia deliciosa ‘AU Fitzgerald’ were collected in December 2018 and 2019 (334 and 360 chilling units, respectively), shortly after leaf abscission. Canes were cut to 10 nodes after removing the first six basal nodes, placed in jars filled with distilled water, and transferred to respective chilling treatments. Treatments included continuous chilling (CC) (in addition to base chilling) at 1-week (168 chilling units) increments (0–5 weeks) and chilling exposure at the same increments with intermittent warm temperature (WT). For the WT treatments, each week of chilling was followed by 3 days of exposure to warm conditions. Chilling and warm temperature exposure were simulated by 7/4 °C and 25/17.2 °C (day/night) air temperatures, respectively, using separate climate-controlled growth chambers. After treatments, canes were forced in a third chamber at 21.1 to 25.0 °C with light-emitting diode lighting. Vegetative budbreak, floral bud number (from here on defined as floral response), and floral development stage were recorded for each cane at 2-day intervals. For ‘AU Golden Dragon’, WT did not result in any reduced floral response at any of the observed chilling levels. However, lower mean floral response was observed with WT, as compared with CC for ‘AU Fitzgerald’ at 5 weeks of chilling over the 2 years (P = 0.05). WT also lessened the effect of apical dominance with respect to vegetative/floral response to node position for both cultivars. Chilling type had no significant effect on vegetative response in either cultivar. Estimated chilling requirements (CC) in this experiment were similar to those reported previously for these cultivars. Results suggest that A. chinensis cultivars may respond more favorably than A. deliciosa to the erratic winter temperature patterns experienced in the southeastern United States.

Genetic and molecular regulation of chilling requirements in pear: breeding for climate change resilience.

Pear (Pyrus spp.) is a deciduous fruit tree that requires exposure to sufficient chilling hours during the winter to establish dormancy, followed by favorable heat conditions during the spring for normal vegetative and floral budbreak. In contrast to most temperate woody species, apples and pears of the Rosaceae family are insensitive to photoperiod, and low temperature is the major factor that induces growth cessation and dormancy. Most European pear (Pyrus Communis L.) cultivars need to be grown in regions with high chilling unit (CU) accumulation to ensure early vegetative budbreak. Adequate vegetative budbreak time will ensure suitable metabolite accumulation, such as sugars, to support fruit set and vegetative development, providing the necessary metabolites for optimal fruit set and development. Many regions that were suitable for pear production suffer from a reduction in CU accumulation. According to climate prediction models, many temperate regions currently suitable for pear cultivation will experience a similar accumulation of CUs as observed in Mediterranean regions. Consequently, the Mediterranean region can serve as a suitable location for conducting pear breeding trials aimed at developing cultivars that will thrive in temperate regions in the decades to come. Due to recent climatic changes, bud dormancy attracts more attention, and several studies have been carried out aiming to discover the genetic and physiological factors associated with dormancy in deciduous fruit trees, including pears, along with their related biosynthetic pathways. In this review, current knowledge of the genetic mechanisms associated with bud dormancy in European pear and other Pyrus species is summarized, along with metabolites and physiological factors affecting dormancy establishment and release and chilling requirement determination. The genetic and physiological insights gained into the factors regulating pear dormancy phase transition and determining chilling requirements can accelerate the development of new pear cultivars better suited to both current and predicted future climatic conditions.

Perspectives on the adaptation of Japanese plum-type cultivars to reduced winter chilling in two regions of Spain.

Japanese plum, like other temperate fruit tree species, has cultivar-specific temperature requirements during dormancy for proper flowering. Knowing the temperature requirements of this species is of increasing interest due to the great genetic variability that exists among the available Japanese plum-type cultivars, since most of them are interspecific hybrids. The reduction of winter chilling caused by climate change is threatening their cultivation in many regions. In this work, the adaptation perspectives of 21 Japanese plum-type cultivars were analyzed in two of the main plum-growing regions in Spain, Badajoz and Zaragoza, to future climate conditions. Endodormancy release for subsequent estimation of chilling and heat requirements was determined through empirical experiments conducted during dormancy at least over two years. Chill requirements were calculated using three models [chilling hours (CH), chilling units (CU) and chilling portions (CP)] and heat requirements using growing degree hours (GDH). Chilling requirements ranged 277-851 CH, 412-1,030 CU and 26-51 CP, and heat requirements ranged from 4,343 to 9,525 GDH. The potential adaption of the cultivars to future warmer conditions in both regions was assessed using climate projections under two Representative Concentration Pathways (RCP), RCP4.5 (effective reduction of greenhouse gas emissions) and RCP8.5 (continuous increase in greenhouse gas emissions), in two time horizons, from the middle to the end of 21st century, with temperature projections from 15 Global Climate Models. The probability of satisfying the estimated cultivar-specific chilling requirements in Badajoz was lower than in Zaragoza, because of the lower chill availability predicted. In this region, the cultivars analyzed herein may have limited cultivation because the predicted reduction in winter chill may result in the chilling requirements not being successfully fulfilled.

Phenology of Apple Cultivars with Different Chilling Requirements

Under mild climate conditions where chilling requirements are not met, the different phenological stages tend to extend and vary according to the year and chilling intensity. Knowledge on the phenological stages becomes important for the management of some cultural practices such as fruit thinning and phytosanitary treatments. The phenology of ‘Condesa’, ‘Imperatriz’, ‘Fred Hough’, ‘Gala’, ‘Fuji Suprema’ and ‘Baronesa’ apple cultivars with different chilling requirements was studied for a period of 20 years. The method employed made it possible to analyze the evolution of the different phenological stages, allowing the chronological determination of their development. The results suggest adaptability of the different cultivars in the same region, highlighting the influence of environmental factors. The duration of the different phenological stages varied according to the chilling requirements of each cultivar, being directly influenced by climatic factors.

Discrepancies in methodologies to determine chill requirements in temperate fruit trees constrain guidelines for future plantings in a global warming context

Temperate fruit trees enter a dormant state and resume growth development once cultivar-specific chill requirements (CR) are fulfilled. Sustainable fruit production requires crucial knowledge of the CR of the varieties to predict the adaptability of crops to future climate conditions. Experimental methodologies based on exposing shoots to forcing conditions, and statistical methods based on analysing long-term phenology records, such as Partial Least Square regression (PLS), are commonly used to estimate CR. To demonstrate the importance of unifying methodologies, we compared CR estimations from six approaches that included bud weight increase (30 % and 50 %), different percentages of bud break (30 %, 50 % and 90 %) after forcing conditions, and PLS regression. The CR of four almond and four apricot cultivars were experimentally and statistically determined in Murcia, South-Eastern Spain. Comparisons between approaches revealed that the choice of the method had a significant effect on the estimates. Large variation in CR estimates was found between PLS regression and the experimental methodologies in some late-flowering cultivars. To assess the adaptation potential of a certain cultivar to future climate conditions based on the CR estimates obtained through the different methodologies, we compared the probability of fulfilling cultivar-specific CR's under two global warming scenarios. Projections revealed a marked decreasing trend of chill availability in the region, which is already threatening the cultivation of genotypes with high CR. This study demonstrates that the variability of methodologies used to quantify CR has an important impact on assessing the probability of a successful adaptation. CR obtained by a certain methodology suggest that insufficient chill might be an increasingly common situation for some cultivars, potentially resulting in production risks. Conversely, other methodologies appear to indicate a more favourable performance for the same cultivar. Implementing standardized methods and metrics is essential to ensure more reliable information regarding the prospects for future crop adaptation.

Transcriptome-Based Identification of Candidate Flowering-Associated Genes of Blueberry in a Plant Factory with Artificial Lighting (PFAL) under Short-Day-Length Conditions.

In blueberry (Vaccinium corymbosum L.), a perennial shrub, flower bud initiation is mediated by a short-day (SD) photoperiod and buds bloom once the chilling requirement is satisfied. A plant factory with artificial lighting (PFAL) is a planting system that can provide a stable and highly efficient growing environment for blueberry production. However, the characteristics of bud differentiation of blueberry plants inside PFAL systems are poorly understood. To better understand flower bud initiation and the flowering mechanism of blueberry in PFAL systems, the anatomical structure of apical buds under SD conditions in a PFAL system was observed using the southern highbush cultivar 'Misty' and a transcriptomic analysis was performed to identify the candidate flowering genes. The results indicated that the apical bud of 'Misty' differentiated gradually along with SD time course and swelled obviously when chilling was introduced. A total of 39.28 Gb clean data were generated, and about 20.31-24.11 Mb high-quality clean reads were assembled, yielding a total of 17370 differentially expressed genes (DEGs), of which 9637 were up-regulated and 7733 were down-regulated. Based on the functional annotation, 26 DEGs were identified including 20 flowering-related and 6 low-temperature DEGs, out of which the expressive level of four flowering-related DEGs (VcFT2, VcFPA, VcFMADS1, and VcCOP1) and two low-temperature-induced DEGs (VcTIL-1 and VcLTI 65-like) were confirmed by qRT-PCR with a good consistency with the pattern of transcriptome. Functional analysis indicated that VcFT2 was highly conserved with nuclear and cytoplasmic subcellular localization and was expressed mainly in blueberry leaf tissue. In Arabidopsis, ectopic overexpression of VcFT2 results in an early flowering phenotype, indicating that VcFT2 is a vital regulator of the SD-mediated flowering pathway in blueberry. These results contribute to the investigation of photoperiod-mediated flowering mechanisms of blueberry in PFAL systems.

Overcoming Dormancy in Prunus Species under Conditions of Insufficient Winter Chilling in Israel.

The phenomenon of dormancy and the evolutionary causes for its development are presented together with the effects of the climatic factors: temperature and light. Shade and darkness have been found to enhance bud breaking in peach. The effects of various temperatures on chilling accumulation, chilling negation and chilling enhancement are described. The way these are computed in the face of global warming is explained, using the dynamic model. When natural chilling is less than that required, there are ways of compensation, up to a certain level. Various horticultural, physical and chemical means to achieve this are described, including bending branches, reducing vegetative vigor, shading the orchard, sprinkling to reduce daytime temperature and the application of various chemicals to break dormancy. When winter chilling is markedly reduced and temperatures increase considerably, the use of dormancy avoidance is suggested in frost-free places. This technique can induce a new growing cycle by avoiding dormancy altogether. However, the best approach is to breed high-quality cultivars requiring much less chilling. Another aspect discussed in this work, independent of the chilling requirement, is the negative effect of heat spells in winter and spring on the abnormal development of flower buds, leading to a low level of the stone fruit set and a reduced yield.

Physiological adaptations of the Japanese plum tree for agricultural productivity: A promising crop for high altitude tropics

The Japanese plum tree is of great importance in the productive development of Colombian fruit growers due to its nutritional contribution to human health, its great adaptability in the tropical highlands, and its good yields. This review presents the current investigative state of physiology of this plant and its management in tropical highlands, including aspects such as its ecophysiology, forced production, gas exchange, flowering, pollination, and fruit development. In Colombia, Japanese plum production systems are adapted between 1,670 and 2,900 m a.s.l., average solar brightness of 1,400 h per year, 12 h photoperiods, temperatures between 14 and 20°C during the day and 6 and 8°C during the night, and rainfall between 700 and 1,600 mm per year. Under these conditions, management can be implemented to produce cyclical crops of the Japanese plum. This management consists of the selection of varieties with low chilling requirement, chemical defoliation, proper fertilization, fruit and green pruning, and the application of chemical substances that promote the breaking of flower buds. Flowering and pollination require a high specificity so that they do not present incompatibility. The growth and development of the fruit requires 1,538 degree days until harvest. This review indicates the great adaptability, management, and production of Japanese plum in the Colombian high tropics.

Characterization of the MIKCC-type MADS-box gene family in blueberry and its possible mechanism for regulating flowering in response to the chilling requirement.

The expression peak of VcAP1.4, VcAP1.6, VcAP3.1, VcAP3.2, VcAG3, VcFLC2, and VcSVP9 coincided with the endo-dormancy release of flower buds. Additionally, GA4+7 not only increased the expression of these genes but also promoted flower bud endo-dormancy release. The MIKCC-type MADS-box gene family is involved in the regulation of flower development. A total of 109 members of the MIKCC-type MADS-box gene family were identified in blueberry. According to the phylogenetic tree, these 109 MIKCC-type MADS-box proteins were divided into 13 subfamilies, which were distributed across 40 Scaffolds. The results of the conserved motif analysis showed that among 20 motifs, motifs 1, 3, and 9 formed the MADS-box structural domain, while motifs 2, 4, and 6 formed the K-box structural domain. The presence of 66 pairs of fragment duplication events in blueberry suggested that gene duplication events contributed to gene expansion and functional differentiation. Additionally, the presence of cis-acting elements revealed that VcFLC2, VcAG3, and VcSVP9 might have significant roles in the endo-dormancy release of flower buds. Meanwhile, under chilling conditions, VcAP3.1 and VcAG7 might facilitate flower bud dormancy release. VcSEP11 might promote flowering following the release of endo-dormancy, while the elevated expression of VcAP1.7 (DAM) could impede the endo-dormancy release of flower buds. The effect of gibberellin (GA4+7) treatment on the expression pattern of MIKCC-type MADS-box genes revealed that VcAP1.4, VcAP1.6, VcAP3.1, VcAG3, and VcFLC2 might promote flower bud endo-dormancy release, while VcAP3.2, VcSEP11, and VcSVP9 might inhibit its endo-dormancy release. These results indicated that VcAP1.4, VcAP1.6, VcAP1.7 (DAM), VcAP3.1, VcAG3, VcAG7, VcFLC2, and VcSVP9 could be selected as key regulatory promoting genes for controlling the endo-dormancy of blueberry flower buds.

Feasting on the ordinary or starving for the exceptional in a warming climate: Phenological synchrony between spongy moth (Lymantria dispar) and budburst of six European tree species.

Global warming is affecting the phenological cycles of plants and animals, altering the complex synchronization that has co-evolved over thousands of years between interacting species and trophic levels. Here, we examined how warmer winter conditions affect the timing of budburst in six common European trees and the hatching of a generalist leaf-feeding insect, the spongy moth Lymantria dispar, whose fitness depends on the synchrony between egg hatch and leaf emergence of the host tree. We applied four different temperature treatments to L. dispar eggs and twig cuttings, that mimicked warmer winters and reduced chilling temperatures that are necessary for insect diapause and bud dormancy release, using heated open-top chambers (ambient or +3.5°C), and heated greenhouses (maintained at >6°C or >10°C). In addition, we conducted preference and performance tests to determine which tree species the larvae prefer and benefit from the most. Budburst success and twig survival were highest for all tree species at ambient temperature conditions, whereas it declined under elevated winter temperature for Tilia cordata and Acer pseudoplatanus, likely due to a lack of chilling. While L. dispar egg hatch coincided with budburst in most tree species within 10 days under ambient conditions, it coincided with budburst only in Quercus robur, Carpinus betulus, and, to a lesser extent, Ulmus glabra under warmer conditions. With further warming, we, therefore, expect an increasing mismatch in trees with high chilling requirements, such as Fagus sylvatica and A. pseudoplatanus, but still good synchronization with trees having low chilling requirements, such as Q. robur and C. betulus. Surprisingly, first instar larvae preferred and gained weight faster when fed with leaves of F. sylvatica, while Q. robur ranked second. Our results suggest that spongy moth outbreaks are likely to persist in oak and hornbeam forests in western and central Europe.

Allelic variation of PmCBF03 contributes to the altitude and temperature adaptability in Japanese apricot (Prunus mume Sieb. et Zucc.).

Japanese apricot is an important subtropical deciduous fruit tree in China, widely distributed in different altitude areas. How does it adapt to the different temperature environments in these areas? In this study, we identified a low-temperature transcription factor PmCBF03 on chromosome 7 through adaptive analysis of populations at different altitudes, which has an early termination single nucleotide polymorphism mutation. There were two different types of variation, PmCBF03A type in high-altitude areas and PmCBF03T type in low-altitude areas. PmCBF03A gene increased the survival rate, Fv/Fm values, antioxidant enzyme activity, and expression levels of antioxidant enzyme genes, and reducing electrolyte leakage and accumulation of reactive oxygen species in transgenic Arabidopsis under low temperature and freezing stress. Simultaneously, PmCBF03A gene promoted the dormancy of transgenic Arabidopsis seeds than wild-type. Biochemical analysis demonstrated that PmCBF03A directly bound to the DRE/CRT element in the promoters of the PmCOR413, PmDAM6 and PmABI5 genes, promoting their transcription and enhanced the cold resistance and dormancy of the overexpressing PmCBF03A lines. While PmCBF03T gene is unable to bind to the promoters of PmDAM6 and PmABI5 genes, leading to early release of dormancy to adapt to the problem of insufficient chilling requirement in low-altitude areas.

Daylength predominates the bud growth initiation of winter deciduous forest trees in the monsoon region of China.

Climate warming has induced significant shifts in spring phenology over both temperate and boreal forests. The timing of bud growth resuming from dormancy is crucial for predicting spring phenology. However, the mechanisms by which environmental cues, other than chilling accumulation, initiate bud growth remains unclear. By constructing a revised process-based spring phenology model incorporating photoperiod and temperature triggers of bud growth, we simulated the first leaf unfolding and first flowering dates of four deciduous forest trees during 1981-2014 at 102 stations across China's monsoon regions. Then, we revealed spatial patterns of the two triggers. Moreover, we compared fitting precision and robustness of the revised model with three mainstream models. Results show that the revised models can effectively simulate all spring phenology time series. Growth initiation of foliar and floral buds was induced by photoperiod lengthening in 80.8% and 77.7% of time series, and by temperature increasing in remaining 19.2% and 22.3% of time series, respectively. The proportions of time series with photoperiod- and temperature-initiated bud growth significantly increase and decrease from northern to southern climatic zones, respectively. Chilling exposure controls the predominant bud growth triggers in different climate zones. Specifically, in regions with long and severe winters where chilling requirement is easily fulfilled, rising temperature in spring alleviates the cold constraint and initiate bud growth. Conversely, in regions with short and mild winters, prolonged daylength in spring compensates the lack of chilling exposure to initiate bud growth. These findings suggest that photoperiod may limit spring phenology response to temperature in low-latitudes. Overall, our model slightly outperforms other models in terms of efficiency, accuracy, and robustness in modeling leaf unfolding and flowering dates. Therefore, this study deepens our understanding of the mechanisms of spring phenology, and improves the predicting capability of spring phenology models in the face of ongoing global warming.

Feasible strategies for efficient propagation system of Paeonia lactiflora ‘Hang Baishao’: In vitro culture of embryos

Herbaceous peony (Paeonia lactiflora Pall.) is a renowned traditional Chinese flower, which has been cherished by people since ancient times. It holds significant value as both an agricultural cash crop and an ornamental and medicinal plant. However, the lack of in vitro propagation system has been long hindered the commercial production of this species and the development of new cultivars. In this study, the low chilling requirement cultivar Paeonia lactiflora ‘Hang Baishao’ that originated from low latitudes of China, was used to study the in vitro culture of embryos. The results of our experiment showed that the optimal medium for in vitro culture of embryos was a 1/2 Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 6-benzyladenine (6-BA), 1.0 mg/L gibberellic acid (GA3) and 2.0 g/L polyvinyl pyrrolidone (PVP). Additionally, histological observations revealed the crucial role of vascular bundle development in the survival of transplants. Plants with well-developed vascular tissue exhibited better transplanting success. This study presents, for the first time, a protocol for embryos propagation and transplanting of ‘Hang Baishao’, a cultivar with low chilling requirement (CR) during winter. This study provides an efficient method for in vitro culture of ‘Hang Baishao’, and makes it possible for large-scale production, utilization, and germplasm preservation of this species.