Salivary Cortisol In Children Research Articles

Demographic, clinical characteristics and psychosocial factors associated with salivary cortisol as a stress biomarker in children with leukemia

Objective: This study aims to determine the relationship among demographic, clinical characteristics, and psychosocial factors related to salivary cortisol in children with leukemia. Methods: This research was a descriptive study with a cross-sectional design. The sample was children with leukemia in Dr. Sardjito General Hospital Yogyakarta from January to February 2018 The sample size was 30 respondents who had met the inclusion criteria, was aged 6-18 years, did not experience an emergency condition, and did not eat and drink 30 minutes before the saliva sample was taken. Salivary samples were assessed for free cortisol levels using an ELISA kit 96 wells DES661.

Early Childhood Education Teacher Psychophysiology and Teacher Stress: Prior Findings, Current Studies, and Future Directions

Early Childhood Education Teacher Psychophysiology and Teacher Stress: Prior Findings, Current Studies, and Future Directions

Can dogs reduce stress levels in school children? effects of dog-assisted interventions on salivary cortisol in children with and without special educational needs using randomized controlled trials.

Prolonged or excessive stress negatively affects learning, behavior and health across the lifespan. To alleviate adverse effects of stress in school children, stressors should be reduced, and support and effective interventions provided. Animal-assisted interventions (AAI) have shown beneficial effects on health and wellbeing, however, robust knowledge on stress mediation in children is lacking. Despite this, AAIs are increasingly employed in settings world-wide, including schools, to reduce stress and support learning and wellbeing. This study is the first randomized controlled trial to investigate dog-assisted interventions as a mediator of stress in school children with and without special educational needs (SEN) over the school term. Interventions were carried out individually and in small groups twice a week for 20 minutes over the course of 4 weeks. We compared physiological changes in salivary cortisol in a dog intervention group with a relaxation intervention group and a no treatment control group. We compared cortisol level means before and after the 4 weeks of interventions in all children as well as acute cortisol in mainstream school children. Dog interventions lead to significantly lower stress in children with and without special educational needs compared to their peers in relaxation or no treatment control groups. In neurotypical children, those in the dog interventions showed no baseline stress level increases over the school term. In addition, acute cortisol levels evidenced significant stress reduction following the interventions. In contrast, the no treatment control group showed significant rises in baseline cortisol levels from beginning to end of school term. Increases also occurred in the relaxation intervention group. Children with SEN showed significantly decreased cortisol levels after dog group interventions. No changes occurred in the relaxation or no treatment control groups. These findings provide crucial evidence that dog interventions can successfully attenuate stress levels in school children with important implications for AAI implementation, learning and wellbeing.

A mother's touch: Preschool-aged children are regulated by positive maternal touch.

Positive maternal touch plays an important role in the development of children's physiological regulation and cognitive development in infancy, as well as the development of sociality in early childhood. However, few studies have looked beyond infancy to consider the possible continuing impact of positive maternal touch on child stress reactivity during early childhood. A diverse community sample of mothers (N=114, Mage =33.52 years, SD=5.33) and their preschool-aged children (Mage =41.68 months, SD=4.67; 49.1% female) participated in the study. Basic demographics were reported by mothers. We coded maternal touch behaviors during an emotionally charged laboratory conversation task and assessed children's physiological reactivity to stressful laboratory tasks with salivary cortisol. Results reveal a significant negative association between positive maternal touch and child salivary cortisol reactivity. In addition, family income, adjusted for family size, and child sex were significantly associated with child cortisol stress reactivity. Findings are discussed in terms of persistent downregulating effects of positive maternal touch on child stress reactivity, as well as possible links of stress reactivity with family income, a proxy for economic stress, and child sex.

The 3D-Transition Study: Objectives, Methods, and Implementation of an Innovative Planned Missing-Data Design.

The prevalence of mental health problems represents a significant burden on school and community health resources as early as preschool. Reducing this burden requires a better understanding of the developmental mechanisms linking children's early vulnerabilities with mental health after the transition to formal schooling. The 3D-Transition Study (2017-2021) follows 939 participants from a pregnancy cohort in the province of Québec, Canada, as they transition to kindergarten and first grade to examine these mechanisms. Biannual assessments include completed questionnaires from 2 parents as well as teachers, parent-child observations, anthropometric measurements, and age-sensitive cognitive assessments. Saliva is also collected on 11 days over a 16-month period in a subsample of 384 participants to examine possible changes in child salivary cortisol levels across the school transition and their role in difficulties observed during the transition. A combination of planned missing-data designs is being implemented to reduce participant burden, where incomplete data are collected without introducing bias after the use of multiple imputation. The 3D-Transition Study will contribute to an evidence-based developmental framework of child mental health from pregnancy to school age. In turn, this framework can help inform prevention programs delivered in health-care settings during pregnancy and in child-care centers, preschools, and schools.

Implicit motives and children's salivary cortisol reactivity to an adapted version of the Trier Social Stress Test for Children (TSST-C)

This research addresses the interplay between two implicit motives, that are power (nPow) and affiliation (nAff), and the cortisol reactivity (CR) to a psychosocial stressor (an adaption of the Trier Social Stress Test for Children; TSST-C; Buske-Kirschbaum et al., 1997) in 89 healthy children (45 female; Mage = 7.74, SDAge = 0.46). We assessed implicit motives by a 6-image Picture Story Exercise (PSE) and cortisol by 6 saliva samples. As hypothesized, the procedure triggered a significant cortisol reaction, F(1.44, 127.05Greenhouse-Geisser) = 8.22, p = .002, η2part = 0.09. Contrary to our hypothesis, children high in nPow showed no significant increase in CR (β = 0.06, p = .60). However, our results were in line with the findings of Wegner, Schüler, and Budde (2014) that a high implicit affiliation motive is associated with an attenuated CR (β = −0.21, p = .05). Perspectives for future research on implicit motives and children's CR to psychosocial stress are discussed.

Economic instability and household chaos relate to cortisol for children in poverty.

The present study investigated relations among various aspects of economic adversity and cortisol levels for young children facing economic hardship. Specifically, the study examined relations to cortisol for variables representing family income, material hardship, financial strain, economic instability, and household chaos. Participants were 374 children, ages 3-5 years, who attended a Head Start preschool, as well as their primary caregivers. Nearly all children lived in households classified as poor or low-income, defined as less than two times the federal poverty threshold. Caregivers completed interviews about family demographics and economic adversity at the beginning of the school year. Child salivary cortisol was sampled in duplicate on two weekday mornings at the end of the school year. We hypothesized that economic instability would show direct statistical effects on child cortisol as well as indirect effects via household chaos. A structural equation model that corresponded to this hypothesis showed adequate fit for the sample data and revealed a statistically significant indirect effect of economic adversity on child cortisol via economic instability and household chaos, as well as statistically significant direct effects of economic instability and chaos on child cortisol, and a significant indirect effect of economic instability on cortisol via household chaos. Implications concern understanding mechanisms of poverty risk, including the impact of instability and chaos on stress physiology, and promoting physiological regulation for children facing economic hardship. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

Comparison of contingent and noncontingent access to therapy dogs during academic tasks in children with autism spectrum disorder.

This study compared contingent and noncontingent access to therapy dogs during educational tasks for children with autism spectrum disorder using a multielement design. The experimenters assessed whether initial preference for the dog predicted reinforcer efficacy and how preference changed across time. A higher response rate during contingent dog sessions than baseline sessions occurred for 4 out of 5 participants, suggesting that the dog functioned as a reinforcer. One participant engaged in a high rate of responding in both contingent and noncontingent dog conditions. Preference assessments revealed idiosyncrasies, suggesting that further research is needed into the predictive nature of initial preference assessments with animals as part of the stimulus array. The experimenters also analyzed salivary cortisol before and after sessions to determine if learning about the upcoming interaction with a dog reduced salivary cortisol in children. Cortisol was variable across participants, with only some deriving a potential physiological benefit from expecting to interact with the dog.

A Double-Blind Randomized Controlled Trial in Effectiveness of Parent-Child Interaction Therapy on Psychological Indicator and Cortisol Level in Children of Caregiver with Cancer

Background: High-potency Cannabis (HPC) is commonly used by patients with cancer to relieve pain. Parents’ HPC consumption can have an adverse effect on the physical, psychological, and social aspects of children. Objectives: This study was conducted aimed to investigate the effectiveness of parent-child interaction therapy (PCIT) on the reduction of aggression and cortisol level in children of dependent cannabis caregiver with cancer. Methods: In a double-blind randomized controlled trial, from March 2015 to October 2016, 50 caregivers residing in Tehran, Iran with metastatic cancer consuming HPC and their children with aggression problem were selected, using respondent-driven sampling (RDS) method and were randomly assigned to the experimental or control groups through block randomization. Changes in the level of aggression and cortisol during 12 weeks were analyzed by repeated measures correlation (rmcorr) and generalized estimation equation (GEE) through SPSS 22 software. Statistical significance was accepted at P < 0.01. Results: The primary outcomes showed that 12 weeks of PCIT had a significant effect on the reduction of children’s aggression and the level of salivary cortisol in children (P < 0.01). However, the results were not stable until the follow-up stage (P = 0.067). Secondary outcomes showed that there was a significant relationship between aggression index and cortisol level (P < 0.01). Conclusions: The findings of the present study are consistent with the research background confirming the role of systematic and nonlinear (cyclic) look at behavioral and psychological problems during growth. These findings can be found in family setting and in educational settings such as kindergartens with clinical application.

Frontal brain delta-beta correlation, salivary cortisol, and social anxiety in children.

Correlated activity of slow-wave (e.g. delta) and fast-wave (e.g. beta) frontal brain oscillations is thought to be an electrophysiological correlate of individual differences in neuroendocrine activity and anxiety in adult samples. We know, however, relatively little about the physiological and functional correlates of delta-beta coupling in children. We examined whether longitudinal patterns of children's basal salivary cortisol and social anxiety across two visits separated by 1year were associated with frontal brain delta-beta correlation in children (Mage =7.59years, SD=1.70). At Time 1 (T1), resting baseline electroencephalogram (EEG) recordings were collected from the children and delta and beta power was measured, and at both T1 and Time 2 (T2), basal salivary cortisol was measured, and parents reported on children's symptoms of social anxiety. Using latent class growth curve analysis, we found that children's salivary cortisol across visits was characterized by a high, stable class (53%), and a low, unstable class (47%), and children's social anxiety was characterized by a high, stable class (50%) and a low, stable class (50%). Using Fisher's r-to-z transformation, we found that frontal EEG delta-beta correlation was significantly stronger among children with high, stable salivary cortisol levels (compared to the low, unstable class; z=2.11, p=.02), and among children with high, stable social anxiety levels (compared to the low, stable class; z=1.72, p=.04). These findings demonstrate that longitudinal patterns of neuroendocrine stress activity and social anxietymay be associated with the correlation of EEG power in slow and fast frontal brain oscillations as early as childhood.

Polygenic risk score of SERPINA6/SERPINA1 associates with diurnal and stress-induced HPA axis activity in children

PurposeCorticosteroid-binding globulin (CBG) transports glucocorticoids in blood. Variation in genes SERPINA6 encoding for CBG, SERPINA2 and SERPINA1 (serpin family A member 6, 2, and 1) have been shown to influence morning plasma cortisol and CBG in adults. However, association of this genetic variation with diurnal and stress-induced salivary cortisol remain unknown. This study aims to investigate the effect of genetic variation in SERPINA6/2/1 loci on diurnal and stress-induced salivary cortisol in children. MethodsWe studied 186, 8-year-old children with genome-wide genotyping. We generated weighted polygenic risk score (PRS) based on 6 genome-wide significant SNPs (rs11621961, rs11629171, rs7161521, rs2749527, rs3762132, rs4900229) derived from the CORNET meta-analyses. Salivary cortisol was measured across one day and in response to the Trier Social Stress Test for Children (TSST-C). ResultsMixed models, adjusted for covariates, showed that the PRS x sampling time interactions associated with diurnal (P < 0.001) and stress-induced (P = 0.009) salivary cortisol. In the high PRS group (dichotomized at median) the diurnal salivary cortisol pattern decreased less from awakening to bedtime than in the low PRS group (standardized estimates of sampling time −0.64 vs. −0.73, P < 0.0001 for both estimates). In response to stress, salivary cortisol increased in the high PRS group while it remained unchanged in the low PRS group (standardized estimates of sampling time 0.12, P = 0.015 vs. −0.06, P = 0.16). These results were mainly driven by minor alleles of rs7161521 (SERPINA6) and rs4900229 (SERPINA1). ConclusionsGenetic variation in SERPINA6/2/1loci may underpin higher hypothalamic-pituitary-adrenocortical axis activity in children.

Trajectories of Social Anxiety in Children: Influence of Child Cortisol Reactivity and Parental Social Anxiety.

Few studies have examined the interactive effect of intra- and extra-individual vulnerability factors on the trajectory of social anxiety in children. In this study, we examined the joint influence of familial vulnerability (i.e., parental social anxiety) and child biological stress vulnerability (i.e., cortisol reactivity) on trajectories of social anxiety. Children (N = 112 (57 males), M age = 8.14years, S.D. = 2.25) were followed over three visits spanning approximately three years. Parental social anxiety was assessed using the Social Phobia and Anxiety Inventory, children's behavior and salivary cortisol reactivity were measured in response to a speech task, and children's social anxiety was assessed at all three visits using the Screen for Child Related Emotional Disorders (SCARED; Parent-report). A growth curve analysis was used to examine trajectories of child social anxiety as predicted by children's cortisol reactivity and parental social anxiety, adjusting for covariates. We found a significant interaction between parental social anxiety and child cortisol reactivity in predicting child social anxiety across time. Having a socially anxious parent coupled with heightened cortisol reactivity predicted the highest levels of child social anxiety, with scores that remained above clinically significant levels for social anxiety across all visits. Children with familial risk for social anxiety and who also exhibit high stress-reactivity appear to be at risk for persistent, clinically significant social anxiety. This highlights the importance of considering the interaction between both biological and contextual factors when considering the development, maintenance, and treatment of social anxiety in children across time.

The social transmission of risk: Maternal stress physiology, synchronous parenting, and well-being mediate the effects of war exposure on child psychopathology.

While chronic early stress increases child susceptibility to psychopathology, risk and resilience trajectories are shaped by maternal social influences whose role requires much further research in longitudinal studies. We examined the social transmission of risk by assessing paths leading from war-exposure to child symptoms as mediated by 3 sources of maternal social influence; stress physiology, synchronous parenting, and psychiatric disorder. Mothers and children living in a zone of continuous war were assessed in early childhood (1.5-5 years) and the current study revisited families in late (9-11years) childhood (N = 177; N = 101 war-exposed; N = 76 controls). At both time-points, maternal and child's salivary cortisol (SC), social behavior, and externalizing and internalizing symptoms were assessed. In late childhood, hair cortisol concentrations (HCC) were also measured and mother and child underwent psychiatric diagnosis. The social transmission model was tested against 2 alternative models; 1 proposing direct impact of war on children without maternal mediation, the other predicting late-childhood symptoms from early childhood variables, not change trajectories. Path analysis controlling for early childhood variables supported our conceptual model. Whereas maternal psychopathology was directly linked with child symptoms, defining direct mediation, the impact of maternal stress hormones was indirect and passed through stress contagion mechanisms involving coupling between maternal and child's HCC and SC. Similarly, maternal synchrony linked with child social engagement as the pathway to reduced symptomatology. Findings underscore the critical role of maternal stress physiology, attuned behavior, and well-being in shaping child psychopathology amid adversity and specify direct and indirect paths by which mothers stand between war and the child. (PsycINFO Database Record

Evaluation of muscle activity, bite force and salivary cortisol in children with bruxism before and after low level laser applied to acupoints: study protocol for a randomised controlled trial

BackgroundBruxism is a repetitive activity that causes tooth wear, audible sounds, and discomfort. Preventive measures have been studied for conditions that can exert a negative influence on physiological development in children. Low-level laser therapy administered over acupoints is an effective, painless, low-cost treatment option that has achieved good results. Thus, the aim of the proposed study is to evaluate changes in muscle activity, bite force and salivary cortisol in children with bruxism after the application of low-level laser to accupoints.MethodsThe children will be randomly allocated to four groups of 19 individuals: G1 - low-level laser; G2 - occlusal splint; G3 - placebo laser; and G4 - control (without bruxism). The BTS TMJOINT electromyography will be used to determine muscle activity and a digital gnathodynamometer will be used to measure bite force. Salivary cortisol will be analysed at baseline as well as one and six months after treatment. Two-way ANOVA will be employed and complemented by Tukey’s test.DiscussionBruxism is a repetitive activity of the masticatory muscles that can have negative consequences if not treated, such as tooth wear, noises, discomfort and anxiety. Thus, control and treatment measures should be taken. Although low-level laser therapy over acupoints has been indicated for children, the effects of this treatment modality have not yet been studied.Trial registrationNCT02757261 on 8 April 2016. This study protocol received a grant from the Brazilian fostering agency São Paulo Research Foundation (FAPESP: #2015/24731-0).

Mechanisms Linking Interparental Aggression to Child Dental Caries

Research has garnered support for a systemic view of factors affecting child dental caries that accounts for the influence of social factors such as the family environment. Our previous work has demonstrated the association between mother-to-father emotional aggression and child caries. The present study builds on these results by evaluating pathways that might explain this relation. Families (n = 135) completed a multimethod assessment of mother-to-father emotional aggression, child caries, and several hypothesized mediators (i.e., child cariogenic snack and drink intake, child internalizing behaviors, child salivary cortisol and α-amylase reactivity, parental laxness, child oral hygiene maintenance, and parental socialization of child oral hygiene maintenance). Mediation analyses partially supported the role of the child's diet as a mechanism linking mother-to-father emotional aggression and child caries. However, children's neglect of oral hygiene, parental laxness, and child emotional and biological disturbances failed to stand as conduits for this association. Future investigations should expand upon these results to better establish the causal links that could only be suggested by the present cross-sectional findings.

Professional dental prophylaxis increases salivary cortisol in children with dental behavioural management problems: a longitudinal study.

BackgroundDental procedures may cause stress and increase the salivary cortisol levels. It is important to known if apparently simple procedures such as professional dental prophylaxis at low speed (DP) are stressful for children with dental behaviour management problems (DBMP) to help with behaviour guidance strategies. This longitudinal study aimed to evaluate if DP changes a physiological marker of stress (salivary cortisol) in children with DBMP who were referred to dental treatment under sedation.MethodsOne paediatric dentist carried out a DP with rubber cup and pumice followed by dental examination in 39 children aged 2–5 years, prior to the dental sedation appointment. Children’s saliva was collected at three different moments: upon waking (UW), on arrival at the dental office reception area (RA) and 25 min after the dental prophylaxis (DP). The saliva samples were analysed using an enzyme immunoassay kit. The Wilcoxon test was used in paired comparison (P < 0.05).ResultsSalivary cortisol levels decreased from UW (0.34; 0.15–0.54) to RA (0.14; 0.08–0.56) (P = 0.019) and increased from RA to DP (0.25; 0.06–1.48) (P = 0.008). Higher salivary cortisol levels were observed at DP when compared to RA in children who did not have previous dental treatment (P = 0.007), had toothache (P = 0.006), presented some protest behaviour during DP (P = 0.008), or needed protective stabilisation by parents for the dental examination (P = 0.005).ConclusionsPaediatric dentists should be aware that even simple procedures such as professional dental prophylaxis are related to stress in young children.

Is arsenic exposure during pregnancy associated with saliva cortisol in 2 years old children from Arica, Chile? Preliminary results.

Introduction: Exposure to inorganic arsenic (iAs) during pregnancy generates lower expression of glucocorticoid receptors in hypothalamus of offspring in murine models. This association has not been studied in children exposed in utero to low concentrations of iAs. The aim is to study the association between low-level iAs exposure during pregnancy and salivary cortisol in 2-years-old children belonging to Arica, Chile. Methods: In this prospective cohort study, a sample of pregnant women was recruited from 5 public health services in Arica. Participation was voluntary and written consent was mandatory. Urine samples to assess total iAs (μg/L) and sociodemographic data were collected from 242 mothers during 2nd trimester of pregnancy. Data related to acute stressful situations and salivary samples, both in children and mothers, were collected when children were around 2-years-old to assess salivary cortisol. Descriptive statistics and multiple linear regression models were fitted to study the association between iAs and salivary cortisol, adjusting by other variables. The modifying effect of PPD variable was evaluated. Results: For these preliminary results we included the analysis of forty-one mother-child pairs. The median of total urine iAs was 14.8μg/L (IQR: 9.2μg/L). The cortisol mean was 0.98μg/dL (SD: 0.19μg/dL). iAs distribution was positively biased and cortisol distribution was symmetric. iAs exposure was transformed in quartile to perform multiple linear regression. iAs was positively associated with salivary cortisol in children whose mothers had postpartum depression (PPD) and inversely associated when the mother did not have PPD. Child’s acute sickness and circadian rhythm were associated with salivary cortisol in children whose mothers had PPD. Conclusions: Preliminary results indicate that iAs was associated with salivary cortisol. However, the addition of a biomarker for acute stressful situations is needed to confirm our preliminary results.

The Trend in Morning Levels of Salivary Cortisol in Children With ADHD During 6 Months of Methylphenidate Treatment

Objective: To determine the trend in cortisol levels in children with ADHD treated with methylphenidate (MPH) and nontreated healthy controls over a 6-month period. Method: The morning salivary cortisol levels of 50 patients with ADHD (40 boys and 10 girls, mean age = 7.6 years) and 50 age- and gender-matched healthy controls were measured at baseline and at 1, 3, and 6 months from baseline. The neuropsychological performance of the ADHD patients was measured via administration of the Continuous Performance Test. Results: The cortisol levels of ADHD patients increased significantly after 1 month of MPH treatment before decreasing to an intermediate level, but were significantly positively correlated with neuropsychological performance throughout the 6-month treatment period. The cortisol levels of the controls did not change significantly over the 6-month period. Conclusion: MPH administration appears to positively influence the functioning of the hypothalamic–pituitary–adrenal axis in ADHD patients.

Stress contagion in the classroom? The link between classroom teacher burnout and morning cortisol in elementary school students

ObjectiveThe purpose of this study was to explore the link between classroom teachers' burnout levels and students' physiological stress response. Drawing from a stress-contagion framework, we expected higher levels of teacher burnout to be related to elevated cortisol levels in elementary school students (N = 406, 50% female, Mean age = 11.26, SD = .89). MethodClassroom teacher burnout was assessed with the Maslach Burnout Inventory modified for teachers. Salivary cortisol was collected as an indicator of students' hypothalamic–pituitary–adrenal (HPA) functioning. We collected salivary cortisol in children at 9 a.m., 11:30 a.m., and 2 p.m. in the classroom setting. ResultsUsing Multilevel Modeling, we found that children's morning cortisol levels significantly varied between classrooms (10% variability). Higher levels of classroom teacher burnout significantly predicted the variability in morning cortisol. Teacher burnout reduced the unexplained variability in cortisol at the classroom level to 4.6%. ConclusionThis is the first study to show that teachers' occupational stress is linked to students' physiological stress regulation. We discuss the present findings in the context of potential stress contagion in the classroom, considering empirical and practical relevance.

Optimizing foster family placement for infants and toddlers: A randomized controlled trial on the effect of the foster family intervention.

The relationship between foster children and their foster carers comes with many risks and may be very stressful both for parents and children. We developed an intervention (foster family intervention [FFI]) to tackle these risks. The intervention focuses on foster children below the age of 5 years. The objective was to investigate the effects of FFI on the interactions between foster parents and foster children. A randomized control trial was carried out with a sample of 123 preschool aged children (mean age 18.8 months; 51% boys) and their foster carers. A pretest was carried out 6 to 8 weeks after placement and a posttest one half year later. Interactions were videotaped and coded using the Emotional Availability Scales (EAS). Foster carers were asked to fill in the Dutch version of the Parenting Stress Index. Morning and evening samples of children's salivary cortisol were taken. In the posttest, significantly positive effects were found on the following EAS subscales: Sensitivity, Structuring, Nonintrusiveness, and Responsiveness. We found no significant differences on stress levels of foster carers and children (Nijmeegse Ouderlijke Stress Index domains and salivary cortisol). This study shows that the FFI has a significant positive effect on parenting skills as measured with EAS and on Responsiveness of the foster child. Findings are discussed in terms of impact and significance relating to methodology and design of the study and to clinical relevance. (PsycINFO Database Record