Chemical Regents Research Articles

Mechanism and molecular level insight of refractory dissolved organic matter in landfill leachate treated by electroflocculation coupled with ozone

Landfill leachate usually contains a substantial content of refractory dissolved organic matter (RDOM), which mainly consists of humic acid and fulvic acid. RDOM easily blocks the membrane pores resulting in membrane fouling when treated using membrane technologies, and it costs abundant chemical regents using advanced oxidation processes. To achieve the economically efficient treatment of RDOM in landfill leachate, this study established the electroflocculation coupled with ozone (EFCO) system. EFCO achieved a higher removal for organic matter than single electroflocculation and ozonation, where RDOM was the main component of organic matter in landfill leachate. The remaining RDOM was decomposed into lots of micromolecule weight and biodegradable proteins and lipids. Ozone improved the removal of dissolved organic matter (DOM) mainly by changing the functional groups on its surface and improving the mechanical strength of flocs to enhance the complexation between flocs and DOM. Cl2− and ClO produced in the EFCO system were the main active chlorine species, which enhanced the mineralization of RDOM. The study provides an efficient method for the pretreatment of RDOM in landfill leachate.

Microbial Responses to Various Types of Chemical Regents during On-Line Cleaning of UF Membranes.

Ultrafiltration is widely used to treat various environmental waters, and on-line membrane cleaning with various chemical reagents is frequently employed to sustain the filtration flux. However, the residue of cleaning agents in the ultrafiltration system is unavoidable, which may affect microbiological properties and biofilm formation during the next-round filtration. By investigating the changes in microbial characteristics, and their biofouling behaviors after exposure to HCl, NaOH, NaClO, citric acid (CA), and sodium dodecyl sulfonate (SDS), this study fills a knowledge gap in microbial responses to various types of chemical cleaning agents in an ultrafiltration system. The result shows that HCl, NaOH, and NaClO affect the bacterial properties and subsequent attachment on the membrane surface, while CA and SDS have no obvious influence on microorganisms. Specifically, HCl, NaOH, and NaClO reduce the hydrophobicity and mean size of suspended microorganisms, increase the extracellular polymeric substances (EPS) release, and trigger intracellular reactive oxygen species (ROS) generation, resulting in the death of a large quantity of microorganisms. Due to the self-protecting strategy, plenty of living cells aggregate on the membrane surface and form a cake layer with a stratified structure, causing more severe membrane biofouling.

Preparation of ultra-conductive bamboo cellulose fiber via a facile pretreatment

The rise of smart textiles has increased the demand for conductive fibers and the common method to realize the conductivity of fibers is to add a layer of nano metal particles via electroless plating process. However, this conventional method often requires redundant pretreatment steps and chemical regents, which hinders the large-scale production of such conductive fibers. Here, we propose a facile one-step alkaline pretreatment to prepare ultra-conductive bamboo cellulose fibers (BCFs) via in-situ reduction of silver nanoparticles (Ag NPs). The Ag-coated BCF has a conductivity of 31,800 S m−1 and a tensile strength of 492 MPa. It also has outstanding flexibility, excellent mechanical stability and washing resistance, maintaining over 96% conductivity after 1000 bending cycles and over 96% Ag retention after 50 washing times. More importantly, this alkaline pretreatment method is suitable for various biomass materials such as wood fibers, and can realize the selective conductivity of materials. This facile, green and universal pretreatment method for preparing conductive biomass fibers is expected to achieve large-scale production and be widely used in the fabrication of smart textiles.

Fluorine-Free Durable Superhydrophobic Surfaces Fabricated via a Simple Spraying Method

Superhydrophobic self-cleaning surfaces are based on the surface micro/nanomorphologies and special chemical regents with low surface free energy. Such surface structures are highly susceptible to mechanical wear. Moreover, the use of fluorinated compounds is harmful to both human health and environment. Herein, a simple method based on spin coating and spraying is proposed to fabricate durable fluorine-free superhydrophobic surfaces. A glass substrate was coated with a layer of water-based paint via a simple spinning process. Due to the adhesive layer, modified SiO2 nanoparticles were strongly bonded to the glass substrate. After the coating was completely cured, the surfaces realized a water contact angle of 165.1° and maintained their water repellency after 50 abrasion cycles with sandpaper and 50 times of stripping test. No chemical reagents harmful for the environment were used.

Self-Powered Respiration Monitoring Enabled By a Triboelectric Nanogenerator.

In mammals, physiological respiration involves respiratory cycles of inhaled and exhaled breaths, which has traditionally been an underutilized resource potentially encompassing a wealth of physiologically relevant information as well as clues to potential diseases. Recently, triboelectric nanogenerators (TENGs) have been widely adopted for self-powered respiration monitoring owing to their compelling features, such as decent biocompatibility, wearing comfort, low-cost, and high sensitivity to respiration activities in the aspect of low frequency and slight amplitude body motions. Physiological respiration behaviors and exhaled chemical regents can be precisely and continuously monitored by TENG-based respiration sensors for personalized health care. This article presents an overview of TENG enabled self-powered respiration monitoring, with a focus on the working principle, sensing materials, functional structures, and related applications in both physical respiration motion detection and chemical breath analysis. Concepts and approaches for acquisition of physical information associated with respiratory rate and depth are covered in the first part. Then the sensing mechanism, theoretical modeling, and applications related to detection of chemicals released from breathing gases are systemically summarized. Finally, the opportunities and challenges of triboelectric effect enabled self-powered respiration monitoring are comprehensively discussed and criticized.

Interior design of hierarchical micro/nanostructures for enhancing energy storage ability of polyanilines through frozen interfacial polymerization

Large specific surface area and hierarchically porous structure play critical roles in the preparation of high–performance electrodes for supercapacitor that strongly depended on the morphologies of electroactive materials. To impart large specific surface area and high ionic permeability, three kinds of hierarchical micro/nanostructures, i.e. sea urchin–like, flower–like and carpenterworm–like nanostructures, of polyaniline (PANI) products were constructed through simple frozen interfacial polymerization without any templates, special chemical regents or organic solvents. The formation of hierarchical micro/nanostructured PANIs were significantly affected by the interfacial area between aniline and oxidant ice layers and the adding order of aniline and oxidant, more detailedly, the number of the nanofibrous seeds formed at the early stage of polymerization and their ambient aniline concentration as well as the release rates of aniline and oxidant. Furthermore, the PANI with sea urchin–like micro/nanostructure exhibited the highest charge storage ability among all the electrode materials, more clearly, the specific capacitance of 716.8 F g–1 at a current density of 1 A g–1, rate retention of 96.9% from 1 to 20 A g–1, and cycling retention of 73.6% after 2000 cycles. The remarkable electrochemical performance can be ascribed to unique self–assembly of highly ordered nanofibers, making it possible to give full play to the advantages of PANI nanofibers including large electrode/electrolyte contact area, high charge transport along the nanofibers, accommodate volume changes extending in every direction during cycling, and fast electron transport and ion diffusion through porous structure. It is believed that such interior design and eco–friendly fabrication can offer an effective strategy to promote the electroactive properties of the PANI, and further development in the sensor– and energy–related fields.

Utilization of palm acid oil for a diffusion combustion burner as fuel and nitrogen oxides reduction by the thermally decomposed hydrocarbons

Palm acid oil (PAO) is considered to be a possible renewable energy. PAO-diesel mixed fuel can keep PAO deposition temperature low, which makes it easy to use as the burner fuel at low risk. However, the use of PAO for combustion fuel and its emission characteristics have not been reported. This study is focused on utilizing PAO as a burner combustion fuel and on finding its emission characteristics including the cause of NO reduction in the burner by PAO. The mixed fuels are prepared and PAO precipitations in the mixed fuel are evaluated. After that, the burner tests were performed. Finally, the cause of the NO reduction in the burner is investigated with a flow reactor. The results show that chemical regents and ultrasound make PAO particle smaller and control a part of PAO deposition. In the burner, 20wt.% PAO having relatively low free fatty acid (LFFA) fuels reduce NOx by up to 30% while keeping high temperature. In the reactor, NO is reduced not only 60% by diesel but also 75∼80% by the PAO (LFFA) at equivalence ratio (φ) =2.63. This study demonstrates that the PAO has potential for NO reduction though complete PAO deposition control is difficult.

Technical solution for supplying a solvent of asphalt-resin-paraffin deposits (AFS) to a tubing string of oil wells equipped with electric centrifugal pumps.

For prevention and combating paraffin deposites different chemical regents are widely used (ihibitors and AFS solvents). In field conditions many ways of chemical regents dosing are applied. Injection of chemical reagents in oilwell tubing is the most effective for ARPD solvents; in this case consumption of reagents is largely decreasing by comparison with chemical reagents dosing through the well-casing annulus. The article describes design and operating processes of technology of ARPD-solvent dosing in electric centrifugal well pumps installation. This process opens fluid access to oilwell tubing at higher pressure at the wellhead. Reagent RT-1-3, is used as aromatic ARPD solvent. In RT-1-3there is butylbenzene fraction of Kazan Production Association Orgsintez containing a mixture of butylbenzene, isopropylbenzene and polyalkylbenzene.

Ultrastable and Highly Catalytically Active N-Heterocyclic-Carbene-Stabilized Gold Nanoparticles in Confined Spaces.

Controlling the size and surface functionalization of nanoparticles (NPs) can lead to improved properties and applicability. Herein, we demonstrate the efficiency of the metal-carbene template approach (MCTA) to synthesize highly robust and soluble three-dimensional polyimidazolium cages (PICs) of different sizes, each bearing numerous imidazolium groups, and use these as templates to synthesize and stabilize catalytically active, cavity-hosted, dispersed poly-N-heterocyclic carbene (NHC)-anchored gold NPs. Owing to the stabilization of the NHC ligands and the effective confinement of the cage cavities, the as-prepared poly-NHC-shell-encapsulated AuNPs displayed promising stability towards heat, pH, and chemical regents. Most notably, all the Au@PCCs (PCC=polycarbene cage) exhibited excellent catalytic activities in various chemical reactions, together with high stability and durability.

Ultrastable and Highly Catalytically Active N‐Heterocyclic‐Carbene‐Stabilized Gold Nanoparticles in Confined Spaces

AbstractControlling the size and surface functionalization of nanoparticles (NPs) can lead to improved properties and applicability. Herein, we demonstrate the efficiency of the metal‐carbene template approach (MCTA) to synthesize highly robust and soluble three‐dimensional polyimidazolium cages (PICs) of different sizes, each bearing numerous imidazolium groups, and use these as templates to synthesize and stabilize catalytically active, cavity‐hosted, dispersed poly‐N‐heterocyclic carbene (NHC)‐anchored gold NPs. Owing to the stabilization of the NHC ligands and the effective confinement of the cage cavities, the as‐prepared poly‐NHC‐shell‐encapsulated AuNPs displayed promising stability towards heat, pH, and chemical regents. Most notably, all the Au@PCCs (PCC=polycarbene cage) exhibited excellent catalytic activities in various chemical reactions, together with high stability and durability.

Technical solution for supplying a wax (deposition) inhibitor to a tubing string of oil wells

For prevention and combating paraffin deposites different chemical regents are widely used (wax inhibitors). In field conditions many ways of chemical regents dosing are applied. Injection of chemical reagents in oil well tubing is the most effective for wax inhibitors; in this case consumption of reagents is largely decreasing by comparison with chemical reagents dosing through the well-casing annulus. The article describes design and operating processes of technology of wax inhibitor dosing in electric centrifugal well pumps installation. This process opens fluid access to oil well tubing at higher pressure at the wellhead. Reagent RT-1-3 is used as aromatic wax inhibitor. In RT-1-3 there is butylbenzene fraction of Kazanorgsintez PJSC containing a mixture of butylbenzene, isopropylbenzene and polyalkylbenzene.

Effectiveness of Process Oriented Guided Inquiry Teaching Strategy on Students’ Performance in Chemistry in Secondary Schools in Ondo State, Nigeria

The Study Examined Qualitative Aspect Of “O” Level West African Secondary School Certificate Practical Chemistry Syllabus And Identified Reaction Of Metals With Water As One Of The Concepts That Chemistry Teachers Avoid To Teach Due To Either Unavailability Of Chemical Regents Or Lack Of Teachers’ Competence To Handle The Topic. The Study Therefore Examined The Effectiveness Of Process Oriented Guided Inquiry Leaving (POGIL) Strategy On Students’ Performance In Chemistry In Secondary Schools In Ondo State, Nigeria. A Pretest, Posttest Control Group Design Was Adopted With POGIL Being the treatment and lecture method as the control group. A total of 60 Senior Secondary School chemistry students (SSS 3) were randomly selected from Akoko South West Local Government Area of Ondo State Nigeria to constitute the study sample. Two intact classes comprising 33 students for POGIL group and 27 students for the lecture method group were taught separately in their respective schools. A 1 item instrument tagged Practical Chemistry Achievement Test (CAAT) with reliability co-efficient of r=0.78, p<0.05 was used to collect data. The results showed that POGIL was more effective (=12.63) than lecture method (=10.84), t=2.97, p<0.05. The study concluded that POGIL strategy is an effective method of teaching practical chemistry concepts in general and the qualitative aspect of practical chemistry in particular.

Effectiveness of Process Oriented Guided Inquiry Teaching Strategy on Students’ Performance in Chemistry in Secondary Schools in Ondo State, Nigeria

The Study Examined Qualitative Aspect Of “O” Level West African Secondary School Certificate Practical Chemistry Syllabus And Identified Reaction Of Metals With Water As One Of The Concepts That Chemistry Teachers Avoid To Teach Due To Either Unavailability Of Chemical Regents Or Lack Of Teachers’ Competence To Handle The Topic. The Study Therefore Examined The Effectiveness Of Process Oriented Guided Inquiry Leaving (POGIL) Strategy On Students’ Performance In Chemistry In Secondary Schools In Ondo State, Nigeria. A Pretest, Posttest Control Group Design Was Adopted With POGIL Being the treatment and lecture method as the control group. A total of 60 Senior Secondary School chemistry students (SSS 3) were randomly selected from Akoko South West Local Government Area of Ondo State Nigeria to constitute the study sample. Two intact classes comprising 33 students for POGIL group and 27 students for the lecture method group were taught separately in their respective schools. A 1 item instrument tagged Practical Chemistry Achievement Test (CAAT) with reliability co-efficient of r=0.78, p<0.05 was used to collect data. The results showed that POGIL was more effective (=12.63) than lecture method (=10.84), t=2.97, p<0.05. The study concluded that POGIL strategy is an effective method of teaching practical chemistry concepts in general and the qualitative aspect of practical chemistry in particular.

An Autotransferable g‐C3N4 Li+‐Modulating Layer toward Stable Lithium Anodes

Commercial deployment of lithium anodes has been severely impeded by the poor battery safety, unsatisfying cycling lifespan, and efficiency. Recently, building artificial interfacial layers over a lithium anode was regarded as an effective strategy to stabilize the electrode. However, the fabrications reported so far have mostly been conducted directly upon lithium foil, often requiring stringent reaction conditions with indispensable inert environment protection and highly specialized reagents due to the high reactivity of metallic lithium. Besides, the uneven lithium-ion flux across the lithium surface should be more powerfully tailored via mighty interfacial layer materials. Herein, g-C3 N4 is employed as a Li+ -modulating material and a brand-new autotransferable strategy to fabricate this interfacial layer for Li anodes without any inert atmosphere protection and limitation of chemical regents is developed. The g-C3 N4 film is filtrated on the separator in air using a common alcohol solution and then perfectly autotransferred to the lithium surface by electrolyte wetting during normal cell assembly. The abundant nitrogen species within g-C3 N4 nanosheets can form transient LiN bonds to powerfully stabilize the lithium-ion flux and thus enable a CE over 99% for 900 cycles and smooth deposition at high current densities and capacities, surpassing most previous works.

Piezoelectric Microchip for Cell Lysis through Cell-Microparticle Collision within a Microdroplet Driven by Surface Acoustic Wave Oscillation.

Cell lysis is an important and crucial step for the detection of intracellular secrets. Usually, cell lysis is based on strong ultrasonic waves or toxic chemical regents, which require a large amount of cell suspension. To obtain high efficiency cell lysis for a small amount of sample, a mechanical cell lysis method based on a surface acoustic wave (SAW) microchip is proposed. The microchip simply consists of a piece of LiNbO3 crystal substrate, interdigitated transducers (IDTs) with 80 pairs of parallel electrodes and 3M Magic Tapes. The modulated input electrical signal is coupled into the substrate through IDTs, which produces an acoustic stream in the droplet on the surface of a substrate. When a biofluid droplet containing cells and microparticles is dropped on the surface of the microchip, the cells and microparticles are accelerated and collide with each other. The fluorescence staining results illustrate that the cell membrane is efficiently destroyed and that proteins as well as nucleic acids inside the cell are released. The experimental results show that this method has a high efficiency and low sample consumption. The potential application is the pretreatment of a small amount of tested sample in a hospital or biolab.

Conformation, allergenicity and human cell allergy sensitization of tropomyosin from Exopalaemon modestus: Effects of deglycosylation and Maillard reaction.

Tropomyosin (TM) from Exopalaemon modestus was glycoprotein, the effects of deglycosylation (enzyme and chemical regents) and Maillard reaction on the conformation, allergenicity and human cell sensitization of TM were investigated. TM had both N-glycan and O-glycan. After deglycosylation and glycation, the α-helix of TM reduced, while other secondary structures promoted. Deglycosylation enhanced TM allergenicity and digestibility, contributed to digests with lower allergenicity, glucose glycation weakened the allergenicity and digestibility of TM, led to digests with weaker allergenicity. Gastric digestion partly reduced allergenicity of digests, intestinal digestion highly reduced digests' allergenicity, gastrointestinal digestion most efficiently weakened the allergenicity of digests. After deglycosylation, the deglycosylated TM exerted stronger activation of basophil (KU812), while glucose glycated TM weakened KU812 activation. Deglycosylation exerted inhibition on colon cell (Caco-2) proliferation and increase of IL-8 release, while glucose glycated TM suppressed Caco-2 proliferation and IL-8 release.

An Electrochemical DNA Sensor for Ultrasensitive Detection of Aspirin Resistance Gene Based on Signal Amplification Strategy of Ru(NH3)63+/MPA/Cys Redox Cycling

1. <span lang=DE style=font-size:11.0pt; color:black;mso-themecolor:text1;mso-ansi-language:DE>Abstract Aspirin resistance, the phenomenon of patient showing no response to aspirin, is characterized by a predominant mutation in the COX-1 gene. Accordingly, patients are routinely screened for mutations in this gene to determine whether they can benefit from aspirin treatment. In this work, we reported a simple and ultrasensitive electrochemical method for COX-1 C50T detection for the first time based on Electrochemical-Chemical-Chemical (ECC) redox cycling. In the procedure of assay, Ru(NH 3 ) 6 3+ is used as the electron acceptor that is electrostatically attracted to Peptide Nucleic Acid (PNA) modified electrodes and new chemical regents of Mercaptopropionic Acid <span style=letter-spacing: -.25pt>(MPA) and cysteamine were introduced, which permits Ru(NH 3 ) 6 3+ to be regenerated for multiple redox cycles. Different Pulse Voltammetry (DPV) was applied to record the electrochemical signals, which increased linearly with the target DNA. Under optimal conditions, the DNA sensors showed a wide linear relationship, from 10 fM to 1 nM, with detection limits of 2.8 fM (S/N = 3). This strategy paves a new avenue for <span style=color:black;mso-themecolor: text1>the determination of aspirin resistance gene in patient serum. 2. Keywords: Aspirin Resistance; COX-1; DNA Sensor; Electrochemical-Chemical-Chemical

Glial differentiation of human inferior turbinate-derived stem cells: a new source of cells for nerve repair.

Schwann cell (SC) transplantation as a cell-based therapy can enhance peripheral and central nerve repair experimentally, but it is limited by donor site morbidity for clinical application. We investigated whether human turbinate-derived mesenchymal stem cells (hTMSCs) isolated from discarded inferior turbinate during surgery can differentiate into functional SC-like cells. hTMSCs expressed mesenchymal cell surface markers CD29, CD44, CD90, and CD105 and did not express neural crest markers P75 and Nestin. After monolayer culture in predifferentiation medium and transdifferentiation medium with a mixture of glial growth factors and chemical regents for 14 days, the differentiated hTMSCs exhibited a spindle-like morphology similar to that of SCs. RT-PCR, immunocytochemical staining, and western blotting analysis indicated that SC-like cells expressed the glial markers S100β, P75, and glial fibrillary acidic protein at the gene and protein level. Compared with hTMSCs, differentiated hTMSCs secreted more neurotrophins, and significantly enhanced the neurite length when cocultured with dorsal root ganglia neuronal cells. Our data indicated that hTMSCs can differentiate into functional SC-like cells and have the ability to facilitate the neurite growth of dorsal root ganglia neuronal cells in vitro, representing a promising source of cells for nerve repair.

Efficient Sterilization with Reactive Oxygen Produced By Radical Vapor Reactor (RVR)

Reactive Oxygen Species (ROS, e.g., ･OH, 1O2, O･2 -) are that the oxygen species with higher reactivity than oxygen molecules (O2) in the atmosphere. ROS provide high reactivity without residual elements except entire O2 and H2O. The advantages of ROS are practically applicable for various purposes, such as solid surface reformation and chemical oxidation. Among them effectiveness of ROS on microorganism sterilizing has been investigated. ROS produced by the macrophages in the body shows a bactericidal effect on bacteria invading from the outside. Thereby we can prevent infection and food poising. As described above, ROS are well known to have a bactericidal action. However, there are not practical ways to produce and expose ROS to objectives. The reason for this may be that the Half-life of ROS is an ephemeral time. Therefore ROS has to be produced high concentration in order to effective exposure. Our laboratory have uniquely conducted research for ROS produce reaction locus, and innovated Radical vapor reactor (RVR) which produces ROS, especially hydroxyl radical (.OH) and singlet oxygen (1O2) with high concentration and instantly. Previous and existing ROS reactors for the production and treatment of these species have been used only in dry atmospheres or in the solution phase. However, in order to produce the hydroxyl radical and related reactive oxygen, water is required as a proton donor. It is important to use the phase interface between the gas (e.g., the plasma phase) and the solution phase (e.g., water) as a reaction locus for the reactive gas and ion(s). In the RVR, ozone was produced by electrical discharge, and the atmosphere in RVR chamber which were containing dispersed water mist and oxygen species (e.g., O2, O3) was irradiated by UV light and maintained at a specific temperature and normal pressure. This RVR reaction is an interfacial reaction between ozone phase gas and water phase with ultraviolet (UV) irradiation. In the interface, produced atomic oxygen abstract hydrogen from water and produce hydroxyl radical and flowing singlet oxygen is produced.[1] RVR can produce ROS instantly and quantitatively without any chemical regents using Radical vapor reaction. Also ROS exposure can be performed with RVR. We have developed some different application of ROS exposing reaction with RVR. It is clean and sustainable non-catalytic reaction that is widely applicable. In this study, we have tried to employ RVR for sterilization of microorganisms. RVR sterilization was evaluated in comparison with other existing sterilizations, e.g., ozone exposure, and UV irradiation. In order to clarify an impact of ROS for sterilization, ROS concentration was assayed quantitatively by spin trap electron spin resonance (st-ESR). 2,2,5,5-tetrametyl-3-pyrroline-3-carboxamide (TPC: highly selective for 1O2) and 5,5-dimetyl-1-pyrroline N-oxide (DMPO : analysis for ･OH and other ROS) was employed as spin trap solutions. These solutions were placed in the RVR reaction chamber. After the RVR reaction by each condition, the spin trap regent solution was analyzed by ESR spectroscopy. Escherichia coli and Bacillus subtilis were employed in order to examine the bactericidal effect of each condition. Bactericidal effect was researched by colony counting after sterilizing treatment. Survival rate was calculated by colony counting. Revealing survival rate and concentration of ROS leads to know the required time for sterilization by ROS. In the results, the RVR sterilization was successfully performed when singlet oxygen and hydroxyl radical were produced and exposed by RVR. Hydroxyl radical was produced much when UV irradiated in RVR, and it was also effective in sterilization. Taking into account of above results, RVR sterilization, i.e. ROS sterilization, is effective to sterilize bacteria. [1] Keishi Matsuo, Yoshiyuki Takatsuji, Masahiro Kohno, Toshiaki Kamachi, Hideco Nakata, and T. Haruyama, Dispersed-phase interfaces between mist water particles and oxygen plasma efficiently produce singlet oxygen (1O2) and hydroxyl radical (･OH), Electrochemistry, 83 (9) , 721-724 (2015) doi: http://dx.xoi.org/10.5796/electrochemistry.83.721

Sophorolipids production from rice straw via SO3 micro-thermal explosion by Wickerhamiella domercqiae var. sophorolipid CGMCC 1576.

A novel lignocellulose material, holocellulose from rice straw via the pretreatment of SO3 micro-thermal explosion, was developed to produce sophorolipids (SLs) with Wickerhamiella domercqiae var. sophorolipid CGMCC 1576. The influence factors of inoculum dose, yeast extract concentration and pH regulators (chemical regents used for adjusting/influencing pH) was investigated and discussed. Results showed that W. domercqiae can grow in the rice straw holocellulose hydrolysate, and acquire relative high SL yield of 53.70 ± 2.61 g/L in shake flask culture. Inoculum dose, yeast extract concentration and pH regulator made obvious influence on fermentation parameters, especially on final broth pH and SLs production. Furthermore, there is a strong negative linear correlation existing between final broth pH and lactonic SL or ratio of lac SL/tot SL. Additionally, comparison between SL production and non-glucose carbon sources, culture methods, microbes in previous reports was carried out. These results will be benefit for acquiring SL mixture with suitable lac SL/tot SL ratio for specific purpose and scope economically.