Chemical Oxygen Research Articles

Enhanced short-chain fatty acid production from sludge anaerobic fermentation by combined pretreatment with sodium pyrophosphate and thermal hydrolysis

The slow breakdown of sludge is the primary obstacle hindering the conversion of waste-activated sludge to short-chain fatty acids (SCFAs) by anaerobic fermentation. This study proposed a novel method incorporating sodium pyrophosphate and thermal hydrolysis (SP-TH) for sludge pretreatment and evaluated its effectiveness regarding SCFA production. The combined pretreatment of SP at 0.4 g/g of total suspended solids and TH at 140 °C enhanced SCFA production from 2,169 ± 208 to 4,388 ± 184 mg chemical oxygen demand/L. SP strips extracellular polymeric substances, and the subsequent TH decomposes cells in the sludge, thus promoting sludge hydrolysis. SP-TH pretreatment promoted SCFA accumulation by enhancing enzyme activity and enriching acidifying bacteria. This study demonstrated that SP-TH pretreatment can effectively promote acid production from sludge, providing a new avenue for organic matter recovery through sludge anaerobic fermentation pretreatment.

Anaerobic treatment of low-strength municipal wastewater with electroactive magnetite-embedded granules under mainstream conditions

This study examined the potential for the anaerobic treatment of low-strength wastewater (250 mg chemical oxygen demand/L) using expanded granular sludge bed (EGSB) with conductive magnetite-embedded granules (MEGs) under mainstream conditions (25°C). The inclusion of magnetite, with high specific gravity and conductivity, enhanced the settleability, structural stability, and electron transfer capability of MEGs compared to granules without it. Within the hydraulic retention time (HRT) range of 4–12 h, the MEG-EGSB reactors maintained superior treatment performance, along with comparable or higher methane production, compared to the control EGSB reactors lacking MEGs. This performance enhancement was more pronounced under conditions of higher hydraulic and organic loading. The MEG-EGSB reactors achieved approximately 90% organic removal efficiency at 8–12-h HRTs, a range comparable to that of the aerobic activated sludge process, with minimal loss of magnetite. The results from RNA-based microbial community analysis, alone with the consistently higher methane content in the biogas from MEG-EGSB reactors, supported the potential development of electric syntrophy between exoelectrogenic bacteria and electrotrophic methanogens in electroactive MEGs. This capability of MEGs to promote electro-syntrophic methanogenesis could potentially enhance methanogenic degradation of organic matter. The overall findings suggest that the MEG-EGSB process is a promising candidate for anaerobic municipal wastewater treatment.

Novel three-sludge municipal wastewater treatment process coupling denitrifying phosphorus removal with anaerobic ammonium oxidation

The two-sludge anoxic dephosphation (DEPHANOX) process frequently encounters the challenge of elevated effluent ammonia levels in practical applications. In this study, the anaerobic ammonium oxidation (anammox) biofilm was introduced into the DEPHANOX system, transforming it into a three-sludge system, enabling synchronous nitrogen and phosphorus elimination, particularly targeting ammonia. Despite a chemical oxygen demand/total nitrogen ratio of 4.3 ± 0.8 in the actual municipal wastewater and 4.5 h of aeration, the effluent total nitrogen was 13.7 mg/L, lower than the parallel wastewater treatment plant. Additionally, the effluent ammonia reduced to 5.1 ± 2.5 mg/L. Notably, denitrifying phosphorus removal and anammox were coupled in the anoxic zone, yielding 74.5 % nitrogen and 87.8 % phosphorus removal. 16S rRNA gene sequencing identified denitrifying phosphorus-accumulating organisms primarily in floc sludge (Saprospiraceae 7.07 %, Anaerolineaceae 1.95 %, Tetrasphaera 1.57 %), while anammox bacteria inhabited the biofilm (Candidatus Brocadia 4.00 %). This study presents a novel process for efficiently treating municipal wastewater.

Sustainable reduction of sulfate contained in gypsum waste: perspectives and applications for agroforestry waste and sanitary sewage

This review article explores sustainable biotechnological strategies for converting sulfate compounds and lignocellulosic waste, focusing on using sulfate-reducing bacteria (SRB) and the valorization of agroforestry residues and sanitary sewage. SRB show potential in effluent treatment, mine drainage, and the removal of sulfate and heavy metals from wastewater, with their metabolic activity being influenced by factors such as pH, temperature, and chemical oxygen demand/sulfate (COD/SO4=) ratio. In the context of a sustainable bioeconomy, the challenge of converting lignocellulosic waste into value-added products is addressed through physical pretreatment techniques such as milling, extrusion, microwave irradiation, and ultrasound, which are efficient in valorizing waste from urban tree pruning. The article highlights the importance of bioreactors in transforming raw materials into desirable biochemical products, discussing different types of bioreactors, such as batch, continuous stirred tank, airlift, fluidized bed, upflow anaerobic sludge blanket (UASB), and bubble column, and their specific advantages and disadvantages. Sustainable sulfate reduction is the central focus, integrating the application of SRB and the conversion of lignocellulosic waste in a way that complements the objectives of the work and promotes a more cohesive flow in the summary. Thus, the interrelationship between effluent treatment strategies and waste valorization is emphasized from an environmental sustainability perspective, highlighting the relevance of this study in the broader context of a sustainable bioeconomy.

Aluminum chloride enhances the production of short-chain fatty acids from waste activated sludge: Insights to performance, mechanism, and implications

Waste activated sludge (WAS), the major by-products during wastewater treatment, is hugely produced, which poses great environment and operation pressures. This work investigated feasibility, mechanism, and implications of aluminum chloride for carbon recovery enhancement from WAS. Results showed that aluminum chloride enhanced short-chain fatty acids (SCFAs) production potential, and the enhancement potentials were dosage dependent. The optimal dosage was 10 mg Al/g total suspended solids (TSS), with the maximum SCFAs production potential of 140 mg chemical oxygen demand/g volatile suspended solids, which was 52.2 % higher compared to that of control. Meanwhile, the compositions of SCFAs were regulated, suggesting that the recovered SCFAs were potential carbon source. Mechanism analysis showed that aluminum chloride increased the release of polysaccharides and proteins by 23.6 % and 71.7 %, respectively, compared to that in control, as well as the removal of extracellular polymeric substances to 27.5 % and the contribution of intracellular release to 38.0 %. This situation facilitated the solubilization of WAS and improved the biochemical degradability of organic matters. In addition, aluminum chloride promoted hydrolysis and acidification stages through affecting the activities of hydrolase, enriching acid-producing microorganisms, and inhibiting the activities of methanogens. This work provided some novel solutions for sludge treatment and carbon recovery, driving wastewater treatment plants to achieve carbon-neutral operation.

Valorization of fruit and vegetable byproducts for the beta-glucan production from Euglena gracilis

Five fruit and vegetable byproducts were evaluated as carbon sources and media for beta-glucan production from Euglena gracilis. Orange peel showed the highest beta-glucan concentration (6.5 g/L) and productivity (1.9 g/L/day) when used as a medium. However, when employed as carbon sources, apple pomace showed the highest beta-glucan concentration (10.6 g/L) and productivity (3.5 g/L/day). The appropriate chemical oxygen demand/nitrogen ratio (71.1) and favorable carbon sources of apple contributed to beta-glucan production. Increasing sugar concentrations in apple pomace and orange peel from 10 to 30 g/L raised the beta-glucan concentration to 11.6 g/L. Using apple pomace and orange peel individually proved more effective than mixing them for beta-glucan production. Therefore, apple as a carbon source is the most effective fruit and vegetables byproduct for beta-glucan production. This is expected to reduce the cost of E. gracilis cultivation on a large-scale and contribute to the circular economy.

Simultaneous biohythane and volatile fatty acids production from food waste in microbial electrolysis cell-assisted acidogenic reactor

To enhance resource recovery from food waste (FW), a semi-continuous experiment was conducted to study simultaneous biohythane and volatile fatty acids (VFAs) production from it in a microbial electrolysis cell-assisted acidogenic reactor (MEC-AR). Anaerobic fermentation of FW at acid pH (3.71–5.19) produced CH4 through acclimating acid-tolerant methanogens. The MEC-AR with 1.2 V and pH 5.04 ± 0.10 simultaneously produced 2.73 ± 0.05 L/L/d biohythane (with 34.80 ± 1.09% CH4, 14.95 ± 0.86% H2, and 28.74 ± 1.11% H2/(H2+CH4)) and 19.39 ± 0.44 g chemical oxygen demand/L VFAs during steady period. The pH of the fermentation liquid and MEC were the primary factors that influenced the production of biohythane and VFAs. No hydrogen was produced in the reactor at low pH (3.50–4.00) or no applied voltage. The MEC also affected the composition of VFAs with high acetic acid (65.43 ± 2.42%) and low propionic acid (3.71 ± 0.76%) contents and increased the relative abundances of Olsenella and Methanobacterium. Energy analysis showed that the MEC improved the energy efficiency of anaerobic fermentation of FW. This study thus provided a novel, efficient, and applicable strategy for resource recovery and valorization of FW.

Study Indicating the Exigency for the Rejuvenation of the Kanamriver in Kannur District, Kerala, India

The Kanam river of the Kannur district originating from Ayyappanmala at Mundery Panchayath, and flows through various areas such as Elayavoor, ThazheChovva, and Kannur corporation limits. The river was filled with soil from the nearby farms and coastal communities become totally spoiled by the wastes. Research works pertaining to this study site is very scanty.In this context, the physico-chemical analysis of the Kanam River, Kannur, Kerala was determined.The objective of the study was to investigate the seasonal variations of physico-chemical parameters such as temperature, pH, transparency, hardness, primary productivity, ammonia, carbon dioxide, dissolved oxygen, biological oxygen demand and chemical oxygen demand.The study indicates that there is a pronounced variation of most of the water quality parameters with variations in season. There are numerous causes including increasing number of industries and various other anthropogenic activities in the neighbouring regions, global climatic change that led to the degradation of the quality of water. The findings of the present study also provide a better understanding of this damaged ecosystem and remind the need for its restoration and it also suggest the need for the starting up of a Rejuvenation strategy.

Performance and stability enhancement of methanogenic granular sludge process: Feed pre-acidification and magnetite-embedded granule formation

Recently, inducing the formation of conductive magnetite-embedded granules (MEGs) by self-embedding submicron magnetite particles into anaerobic granules has been demonstrated to be effective in enhancing methanation and improving sludge stability in upflow anaerobic sludge blanket (UASB) reactors. However, MEGs still suffer structural and functional destabilization at high organic loading rates (OLRs) due to the excessive secretion of extracellular polymeric substances (EPS), resulting in a significant washout of active microbes. This is particularly true when treating carbohydrate-rich wastewater that is prone to producing excessive EPS. In order to address this issue, this study explored the potential of combining MEG-UASB process with feed pre-acidification as a means of improving the methanogenic treatment performance and stability of whey, a carbohydrate-rich dairy effluent. Two UASB reactors, one with MEGs and one without, were operated to treat pre-acidified whey at increasing OLRs of 2–11 g chemical oxygen demand/L·d. Feed pre-acidification was effective in preventing granule destabilization and maintaining stable UASB performance with or without MEGs. Meanwhile, the reactor with MEGs (JM) showed significantly better performance and stability than the one without MEGs (JC), especially at high OLRs, which was likely because MEGs improved sludge retention and promoted electro-syntrophic methanogenic activity. Accordingly, JM sludge exhibited greater electron transfer capability and a more pronounced enrichment of electroactive microbes than JC sludge. External voltage application (0.6 V) did not relieve but rather aggravated the process upset under overloading conditions for both reactors. The overall results suggest that the MEG-UASB process coupled with pre-acidification holds promise for efficient and stable treatment of carbohydrate-rich wastewater.

Application of mathematical models for predicting the trihalomethanes content in drinking water in the city of Kumanova, North Macedonia

Trihalomethanes (THMs) are created as a result of the reaction between chlorine used to disinfect drinking water and natural organic matter in water. At high levels, THMs have been associated with cancer. As a consequence, THMs must be constantly monitored. They are mainly determined by the method of gas chromatography, which is a more difficult procedure and at a higher cost. In recent years, however, mathematical models have been used to predict THMs. These models work by measuring some physico-chemical parameters of drinking water, those values ​​of these parameters are replaced in mathematical models and the THMs content in drinking water can be predicted. The main purpose of this paper was to predict the content of THMs in the drinking water of the city of Kumanova. The measured parameters were: temperature, residual chlorine, pH, electrical conductivity, chemical oxygen, total dissolved solids and chlorides. Measurements were made during the spring season 2022 in the four sampling points. Ten mathematical models were used for prediction and of them the average value with standard deviation of THM was 26.9532 ± 10.03 μg/L. From the result we can conclude that content of THM does not pose a risk to the health of the population.

Efficient ammonia-nitrogen removal and recovery from wastewater via the continuous flat-sheet gas-permeable membranes reactor pretreatment

Ammonia‑nitrogen is a crucial component of landfill leachate and a major factor in the eutrophication of aquatic bodies. A novel approach was developed using the continuous flat-sheet gas-permeable membranes (GPMs) reactor pretreatment to efficiently remove and recover ammonia‑nitrogen. The total ammonia‑nitrogen removal efficiency reached 74.2 ± 4.5 % and 66.2 ± 4.9 %, respectively, when simulated nitrogenous wastewater and landfill leachate were treated under the following conditions: influent flow rate 25 L/d, pH 11.0, temperature 25 °C, sulfuric acid absorption concentration 2.5 %, and sulfuric acid absorption flow velocity 70 mL/min. In addition, (NH4)2SO4 (i.e., a crucial ammonia resource) was recovered efficiently, and the flat-sheet GPMs exhibited stable hydrophobic properties. Specifically, the combination of membrane pretreatment and biological treatment reduced the ammonia‑nitrogen concentration from ~230 mg/L (with direct biological treatment only) to ~40 mg/L, with the removal efficiency benefitting from the increase in the chemical oxygen demand/total ammonia nitrogen (COD/TAN) ratio of the leachate after membrane pretreatment. Furthermore, microorganisms that are capable of ammonia‑nitrogen removal (Planktosalinus and Rheinheimera) were selectively enriched. Overall, this study provides a practical method to enhance ammonia‑nitrogen removal and recovery for the treatment of wastewater containing high ammonia‑nitrogen concentrations.

Insights into the effects of operating parameters on sulfate reduction performance and microbial pathways in the anaerobic sequencing batch reactor

Sulfate-reducing bacteria (SRB)-based anaerobic process has aroused wide concern in the treatment of sulfate-containing wastewater. Chemical oxygen demand-to-sulfate ratio (COD/SO42−) and HRT are two key factors that affect not only the anaerobic treatment performance but also the activity of SRB. In this study, an anaerobic sequencing batch reactor was constructed, and the effects of different operating parameters (COD/SO42−, HRT) on the relationship of sulfate (SO42−) reduction performance, microbial communities, and metabolic pathways were comprehensively investigated. The results indicated that the SO42− removal rates could achieve above 95% under different operating parameters. Bioinformatics analysis revealed that microbial community changed with reactor operation. At the genus level, the enrichment of Propionicclava and Peptoclostridium contributed to the establishment of a homotrophic relationship with Desulfobulbus, the dominant SRB in the reactor, which indicated that they took vital part in maintaining the structural and functional stability of the bacterial community under different operating parameters. In particular, an increasing trend of the relative abundance of functional genes encoding dissimilatory sulfate reduction was detected with the increase of COD/SO42−, which indicated high SO42− reduction potentials. This knowledge will help to reveal the mechanism of the effect of operating parameters on the anaerobic sulfate removal process, thus providing effective guidance for the targeted regulation of anaerobic sequencing batch bioreactors treating SO42--containing wastewater.

Denitrification performance, bioelectricity generation and microbial response in microbial fuel cell – constructed wetland treating carbon constraint wastewater

For the deep reduction of nitrogen, the microbial fuel cell-constructed wetland (MFC-CW) was conducted for treating carbon constraint wastewater. Results indicated that nitrogen removal decreased from 94.96% to 24.96% with influent COD/TN (chemical oxygen demand/total nitrogen) from 4 to 0. MFC-CW was seriously affected by low organic wastewater. Wetland plants contributed to denitrification, with TN removal increasing from 46.13% to 64.87%. The bioenergy output showed a linear relationship with influent COD, and the maximum power density of 1.17 mW/m2 was obtained. Correlation analysis indicated that functional genera of Paenibacillus, Trichococcus, norank_KD4-96, norank_OLB14 played a crucial role in nitrogen removal. Influent COD and wetland plants affected carbon and nitrogen metabolisms, and key genes related to denitrification were more abundant in the cathode. Findings illustrated the nitrogen metabolism in MFC-CW with carbon constraint wastewater and will extend the application of MFC-CW in secondary effluent treatment from wastewater treatment plants.

Zeytinyağı Üretim Atıklarının Biyolojik Aktiviteleri ve Gıdalarda Kullanım Potansiyeli

During the production of olive oil two types of waste are generated: wastewater and olive pomace. Since these wastes have high biochemical and chemical oxygen needs, they have harmful effects on the environment. However, in addition to these damages, many bioactive compounds in olive are transferred to wastewater and pomace during oil processing. Hydroxytyrosol, tyrosol and oleuropein are bioactive components that are dominantly found in both olive oil wastewater and pomace, and these compounds have many bioactivities that positively affect human health. As a result of scientific studies, it has been proven that hydroxytyrosol, tyrosol and oleuropein compounds exhibit various bioactivities such as antioxidant activity, anti-inflammatory effect, cardioprotective effect, neuroprotective effect, chemoprevention properties, antidiabetic effect, antimicrobial and antiviral effect. These wastes are recycled in various sectors such as food, cosmetics and pharmaceuticals due to the bioactive components they contain. It has been reported by scientific studies that wastewater and pomace contain phenolic compounds, pectic polysaccharides and fibers. These can be used as natural preservatives, antioxidants, food enrichers, packaging materials in the food industry. In this review, the production methods, chemical and biological properties of olive oil wastewater and pomace and studies investigating the use potential of these wastes in the food sector are compiled.

An assessment of how the properties of pyrochar and process thermodynamics impact pyrochar mediated microbial chain elongation in steering the production of medium-chain fatty acids towards n-caproate

Microbial chain elongation fermentation is an alternative technology for medium-chain fatty acid (MCFA) production. This paper proposed the addition of pyrochar and graphene in chain elongation to improve MCFA production using ethanol and acetate as substrates. Results showed that the yield of, and selectivity towards, C6 n-caproate were significantly enhanced with pyrochar addition. At the optimal mass ratio of pyrochar to substrate of 2 g/g, the maximum n-caproate yield of 13.67 g chemical oxygen demand/L and the corresponding selectivity of 56.8% were obtained; this represents an increase of 115% and 128% respectively as compared with no pyrochar addition. Such improvements were postulated as due to the high electrical conductivity and surface redox groups of pyrochar. The optimal ethanol to acetate molar ratio of 2 mol/mol achieved the highest MCFA yield under pyrochar mediated chain elongation conditions. Thermodynamic calculations modelled an energy benefit of 93.50 kJ/mol reaction for pyrochar mediated n-caproate production.

Mechanisms of calcium ion inhibiting alkaline fermentation of waste activated sludge for short-chain fatty acids production

Alkaline fermentation is an effective way for short-chain fatty acids recovery from waste activated sludge. However, there is always a high content of calcium ions in waste activated sludge, which may inhibit alkaline fermentation resulting in a low short-chain fatty acids production. The inhibition effect and mechanisms of calcium ions on short-chain fatty acids production from waste activated sludge was investigated and studied in this work. The results showed that when the calcium ions concentration increased from 0 to 5000 mg/L, the short-chain fatty acids production decreased from 307.8 ± 0.7 to 226.8 ± 1.1 mg chemical oxygen demand/g volatile suspended solid. Mechanism study revealed that about 2-fold extracellular polymeric substances were secreted with 5000 mg/L calcium ions addition than that of calcium ions absence to resist the toxic effect of calcium ions, thus inhibiting waste activated sludge disintegration, and less organic substrate were released for the short-chain fatty acids production. Furthermore, flocculation of calcium ions and short-chain fatty acids reduced the short-chain fatty acids concentration in fermentation liquid. • 5000 mg/L calcium inhibited fermentation with 26.3% reduction of SCFAs production. • Aggregation effect of calcium on sludge resulting in the fermentation inhibition. • Complexation of EPS with calcium caused 2-fold EPS remaining in sludge cells. • Flocculation effect of calcium results in SCFAs in liquid lower than expected.

Removal of nitrate from agricultural runoff in biochar electrode based biofilm reactor: Performance and enhancement mechanisms

A biochar electrode based biofilm reactor was developed for advanced removal of nitrate from agricultural runoff. The corn-straw (Zea mays L.) biochar formed at 500 °C has an adsorption capacity of NO3−-N up to 2.659 mg g−1. After 45-day start-up phase, the removal efficiency of nitrate reached 93.4% when impressed current was 20 mA, hydraulic retention time was 12 h and chemical oxygen demand/total nitrogen (C/N) ratio was 0.56 without additional carbon source. In comparison, neither electrochemical reduction alone nor microbial denitrification alone could obtain the ideal nitrate removal efficiency. The results implied that bio-electrochemical reduction was the main way of nitrate removal in the biofilm electrode reactor (BER). The denitrification efficiency of 88.9% could still be obtained when C/N = 0. It is because biochar can significantly promote the utilization efficiency of cathode electrons by microorganisms. Thus, biochar is a promising electrode material, which provides a new idea for the optimization of BER.

Dynamics of indicators of chemical and biochemical oxygen consumption in the Southern Bug river for 2016–2020

The analysis of modern ecological problems of the Southern Bug river basin is carried out, which allows to reveal the most painful ecological threats. Ways to solve these problems and optimize the management of environmental safety of water resources of Vinnytsia region are proposed. The article examines the dynamics of chemical and biochemical oxygen consumption in the Southern Bug basin and its main tributaries, starting in 2016 and ending in 2020. There is a tendency to increase the value of BOD5 annually in drinking water intakes of the Southern Bug basin in Kirovohrad region, which indicates an increase in water pollution of the rivers Ingul, Sukhoklia and Chorny Tashlyk organic compounds. It is shown that a significant part of organic pollution, estimated by BODp, is formed by the three largest cities: Vinnytsia, Khmelnytsky and Kropyvnytskyi. The total share of these cities among the studied point sources reaches 74% of the total amount of discharged organic matter identified by monitoring. Also, the pollution of the Southern Bug River with organic compounds is a consequence of their entry into surface waters from peatlands and swamps. The share of industry in surface water pollution, compared to utilities; industrial wastewater contains almost nine times less organic matter. The main sources of industrial pollution are Ladyzhyn TPP, Ingul and Smolin mines, JSC BOD in Voznesensk.

Cold temperature mediated nitrate removal pathways in electrolysis-assisted constructed wetland systems under different influent C/N ratios and anode materials

Electrolysis had proven to be useful for the enhanced performance in constructed wetlands (CWs). While at cold temperature, the nitrate removal pathways, plant physiological characteristics and microbial community structure in electrolysis-assisted CWs were unclear. Therefore, the purification performance of three electrolysis-assisted horizontal subsurface-flow constructed wetlands (E-HSCWs) with different anodes and a control system in cold seasons were evaluated in this study. E-HSCWs showed a 2.02–83.21% increase of total nitrogen (TN) removal when compared to control, and the gaps were enlarged with increasing C/N (chemical oxygen demand/total nitrogen, COD/TN) ratios. Nitrite accumulation in E-HSCWs presented a first increase then went down trend with increasing C/N ratios, compared to a steady increase in control system. The optimum C/N ratio was 8 in E-HSCWs for both TN and COD removal. Moreover, Ti|IrO2–Ta2O5 (Ti) anode showed the highest potential for TN and COD removal. Less root weight, shorter root length and reduced TN and total phosphorus (TP) contents in roots were observed in wetland plants (Iris sibirica) of E-HSCWs. In E-HSCWs with Fe and C anodes, the nitrate removal was mainly accomplished by autotrophic denitrifier Hydrogenophaga. While in E-HSCWs with Ti anode, the synergistic effect of autotrophic denitrifier Hydrogenophaga and heterotrophic denitrifiers Acidovorax, Simplicispira, Zoogloea accounted for the nitrate removal. These results showed that E-HSCWs at proper C/N ratio of 8 would be promising for nitrate removal at cold temperature.

An investigation into value of Eutrophication in Hosur Lakes

Abstract: Our current concern is that nitrogen and phosphorus amounts are rising rapidly, Otherwise, to limit the growth of algae and biomass, it exists in relatively low concentrations in unmodified natural waters. This investigation aims to attempt to measure the level of eutrophication in the Hosur LakesFor this research, we chose three lakes named Chandrakudi Lake, Doddan Lake and Kelavarapalli dam. For dissolved orthophosphate, chemical oxygen need, organic nitrogen, free ammonia, inorganic nitrogen whole phosphorus and soluble oxygen, etc. some exemplars were gathered and examined. The onsite research as transparence, facts on killing fish and deterioration of the lakes conditions, etc. was also accomplished. The eutrophication rate in the lakes was measured using the Wetzel's plan. The results showed that the rate of eutrophication was very high in all three lakes, i.e. more than enough to very rich in nutrients that cause excessive plant growth, which chokes out the animal life in the water.