This study aimed to determine effect of various levels of protein, with two formulations and herbal sources of methionine and lysine. and synthetic threonine on serum biochemical properties, egg quality traits and egg chemical composition of 160 45-week-old Lyman Brown hens. It was randomly distributed to 8 treatments with five replications in (cages in the battery system), each repetition containing four birds, and the food treatments were, T1: basal diet (control) that contained 16.32% Cruz Protein (CP) with Synthetic 0.41% Methionine (S. T3: Basic Diet (16.18% CP) with (0.42% S.Mthionine), (0.05% Herbal Lysine: HL) and (0.106% S.Therionine) T4: Basic diet (16.18% CP) with (0.42% H.methionine), (0.05% lysine) and (0.106% threonine), T5: Basic diet (15.45% CP) with (o.42% S.methionine), 0.12% S.Lysine) and (0.116% S. threonine) T6: basic diet (15.46% CP) with (0.42% H.methionine), (0.045% H.Lysine and S. threonine), T7: diet Basal Diet (15.12% C.P) with (0.148% S.methionine, (0.150% S.lysine) and (0.130% S.thrionine), T8: Basal Diet (15.12% CP) with (0.148% H.methionine), (0.150% H.lysine) and (0.130% S.thrionine). The results of the statistical analysis in this experiment showed that there were no significant differences in the rate of egg production, the rate of egg mass, and the feed conversion coefficient, while significant differences were found in the rate of egg weight, the rate of feed consumption, and the rate of conversion of each of Protein, methionine, lysine and threonine.
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