Amino Acid Chelates Research Articles

In defense of platinum: Amino acidic polydentate ligands as a shield against sulphur-donor nucleophilic attacks

The interactions of sulfur-containing peptides and proteins with platinum-based anticancer agents are of significant interest in the field of bioinorganic chemistry. We previously modified the lead compound potassium {trichlorido[η2-(but-3-en-1-yl)-2-acetoxybenzoate]platinate(II)} (Pt-butene-ASA) by exchanging two chlorido ligands for an amino acid chelating ligand to improve its stability in aqueous solutions. In the current study, we demonstrate how this amino acid ligand strengthens the inertness of this class of complexes against sulfur-containing compounds. We incubated four complexes, each bearing an amino acid chelating ligand, with various sulfur-containing compounds of biological interest, such as DMSO, methionine, glutathione, and substance P. The reactions were monitored using NMR spectroscopy and mass spectrometry, allowing the identification by combination with IR ion spectroscopy of exotic pentacoordinate platinum(II) complexes as reaction intermediates. The results indicated that the reactivity of complexes 1–4 ((ethene,N-trans)(l-alaninato-N,O)chlorido(η2-ethene)platinate(II), (ethene,O-trans)(β-alaninato-N,O)chlorido(η2-ethene)platinate(II), (l-histidinato-N,N)chlorido(η2-ethene)platinate(II), and (olefin,N-trans)(l-alaninato-N,O)chlorido(η2-(but-3-en-1-yl)-2-acetoxy benzoate)platinate(II), respectively) is determined by the inner coordination sphere, and the addition of an amino acid ligand significantly reduces the rate of nucleophilic substitutions by sulfur-containing nucleophiles. In particular, the N,ethylene-trans isomers reacted only to a low extent with the free thiol in glutathione, while the O,ethylene-trans isomer was completely consumed within 2 h. Complex 4, the new lead compound, showed low reactivity towards S-nucleophiles in general, which confirms the concept of using amino acids as biocompatible bidentate ligands to adjust the reactivity and stability of this kind of metallodrugs.

Computational and crystallographic study of hydrogen bonds in the second coordination sphere of chelated amino acids with a free water molecule: Influence of complex charge and metal ion

Hydrogen bonds of glycine complexes were calculated using quantum chemistry calculations at M06L-GD3/def2-TZVPP level and by analyzing the crystal structures from the Cambridge Structural Database (CSD). One hydrogen bond where amino acid plays the role of the H-donor (NH/O), and two where it plays the role of the H-acceptor (O1/HO, O1 is a coordinated oxygen atom, and, O2/HO, O2 is a non-coordinated oxygen atom) were investigated. The calculations were done on octahedral nickel(II), square pyramidal copper(II), square planar copper(II), palladium(II), and platinum(II) glycine complexes with different charges adjusted using water(s) and/or chlorine ion(s) as the remaining ligands. For NH/O hydrogen bond, interaction energies of neutral complexes are the weakest, from −5.2 to −7.2 kcal/mol for neutral, stronger for singly positive, from −8.3 to −12.1 kcal/mol, and the strongest for doubly positive complex, −16.9 kcal/mol. For O1/HO and O2/HO interactions, neutral complexes have weaker interaction energies (from −2.2 to −5.1 kcal/mol for O1/HO, and from −3.7 to −5.0 kcal/mol for O2/HO), than for singly negative complexes (from −6.9 to −8.2 kcal/mol for O1/HO, and from −8.0 to −9.0 kcal/mol for O2/HO). Additionally to the complex charge, metal oxidation number, coordination number, and metal atomic number also influence the hydrogen bond strength, however, the influence is smaller.

Computational (DFT) and Experimental (NMR) Study of the Chelation of an Iridium Hyperpolarization Transfer Catalyst by Amino Acids

AbstractNon‐hydrogenative Para‐Hydrogen Induced Hyperpolarization (nhPHIP) is a Nuclear Magnetic Resonance (NMR) hyperpolarization technique which has experimentally been used to analyze complex biological samples containing amino acids using the Ir‐IMes hyperpolarization transfer catalyst. A computational study based on Density Function Theory (DFT) was performed on all relevant stereoisomers of [Ir(H)2(IMes)(AA)(Py)] (with AA=glycine, alanine, valine; Py=pyridine), for which R/S chirality and orientation of the amino acid chelation (C/A) were considered. A total of 30 structures were calculated comprising of 6 stereoisomers for achiral glycine, and 12 stereoisomers for each of the chiral amino acids. The abundances derived from the DFT energies confirmed the trends observed in thermal (non‐hyperpolarized) NMR experiments. Additionally, theoretical calculations of electronic (Wiberg bond indices, Natural Bond Orders, Frontier Orbital Analysis), bond dissociation energies, transition states, and activation energies related to interconversion between binding modes, and steric factors (Solid angle) were performed to provide detailed explanations for NMR experimental observations.

Optimization of Chelating Process of Amino Acids of Leather Waste and Glycine with Different Minerals and Its Relationship with Arthrospira platensis Biological Activities

There are several factors were taken in consideration such as time, ratio, temperature and pH to improve the chelation process between the amino acids and inorganic ions. The current study aims to optimize the physical factors controlling the synthesis of chelated amino acids with different minerals and to enhance antioxidant and biodiesel production from Arthrospira platensis cultivated in culture enriched with different chelated minerals. In this study; various physical factors such as (ratio of amino acids and minerals; temperature, time and pH) were used for optimization of chelation formation. The blue -green alga Arthrospira platensis was cultivated under different synthesized chelated minerals (T1-T13), the growth rate, antioxidant, antiradical and biodiesel production were determined in all treated alga. The obtained results showed that the optimum conditions for production of chelated amino acid were ratio (2:1 M), temperature at 60 °C, the duration between 4:5 days and the suitable or stability of chelation at pH = 4, Also, the growth rate of A. platensis with Cu-glycinate higher than Cu-leather waste and Zarrouk media. The antioxidant activity results of different extracts of Arthrospira platensis showed that the water extract gave high antioxidant activity against DPPH radical assay than acetone extract in all treatments when compared with untreated culture (Zarrouk’s medium). Arthrospira platensis cultivated on Zarrouk medium supplemented with chelated amino acids with metals was showed an increase in algal pigments and lipids with Mn-LW, Zn-LW and Mg-LW treatments. Also, the results showed that the produced biodiesel was observed with M-LW treatments, which was more than that of glycinate treatments, untreated culture (Zarrouk) and LW biodiesel. Therefore, the highest biodiesel percentages were founded with Zn, Mn and Mg-LW (5.37, 5.25 and 4.86% respectively). The recorded results and material balance data concluded that possibility for use the chelated minerals (glycine and leather wastes) as plant fertilizer in future because its high yield and low fees for production.Graphical

Can copper amino acid chelates reduce the physiological strain of growing rabbits under summer conditions?

This study was conducted at the Poultry Research Centre, Faculty of Agriculture, Alexandria University, Egypt, during the summer season (July and August months) 2018. For five weeks, until 63 d of age, a total number of 140 weaned V line rabbits were randomly distributed into seven groups of 20 rabbits each. Rabbits of groups 2 and 3 were fed basal diet plus copper sulphate (100 and 200 mg Cu-Sul/kg, respectively). Groups 4 and 5 were fed basal diet plus copper methionine (100 and 200 mg Cu-Meth/kg, respectively) and groups 6 and 7 were fed basal diet plus copper glycine (100 and 200 mg Cu-Gly/kg, respectively), while the first group was fed basal diet only as control. Rabbits fed diet supplemented with higher level (200 mg/kg) of Cu-Meth or Cu-Gly chelates had significantly higher body weight and daily body weight gain (P<0.05) compared to those fed diets supplemented with both levels of Cu-Sul. Rabbits fed diets supplemented with Cu-Meth or Cu-Gly chelates had significantly increased (P<0.05) red blood cells, lymphocyte, high-density lipoprotein and Cu values in blood. Within the studied Cu sources, serum total lipids values of rabbits were significantly lower than in the control group. However, serum total antioxidant capacity, glutathione peroxidase and superoxide dismutase were higher by both organic chelates studied than other groups. In general, copper supplementation improves productive performance and physiological status under summer conditions.

Effects of Different Nutritional Zinc Forms on the Proliferation of Beneficial Commensal Microorganisms

This study compared the minimal inhibition concentrations (MICs) and their effects on the growth kinetics of seven different types of zinc (Zn) compounds and Na2EDTA in the case of three typical commensal beneficial microorganisms (Bacillus subtilis, Lactococcus lactis, and Saccharomyces cerevisiae). The seven Zn compounds included ZnSO4, four Zn–amino acid chelates, and two Zn–EDTA complexes. Both MICs and growth kinetic parameters indicated that different microorganisms show different sensitivities; for example, B. subtilis, L. lactis, and S. cerevisiae were most sensitive to ZnSO4, Na2EDTA, and Zn(NH3)2(Gly)2, respectively. Both ZnEDTA and Zn(NH3)2(Lys)2 improved the growth rate of all beneficial commensal intestinal microorganisms at low concentrations (5–10 mg/L) and showed low toxicity towards all tested strains. At higher concentrations (100–500 mg/L), all compounds decreased the growth rate and increased the lag phase. In conclusion, both growth kinetic parameters and MICs tested effectively measured the inhibitory effects of the test materials; however, growth kinetics provides a more detailed picture of the concentration-dependent effects and those on the mechanisms of microbial growth inhibition.

Rice growth improvement, bio-fortification, and mitigation of macronutrient requirements through foliar application of zinc and iron- glycine chelate and zinc sulfate

Foliar application of Zinc (Zn) and Iron (Fe) fertilizers is known to be an effective solution to enhance rice grain yield and bio-fortification. The objectives of the current field experiment were to evaluate the effects of the foliar application of 0.5, 1, and 1.5 kg ha−1 of Zn and Fe glycine amino acid chelates and ZnSO4 on rice grain yield, protein, Zn, and Fe content of head rice (Hashemi cultivar) under both in the recommended and ½ of the recommended dose of NPK. The highest increase in the grain yield was recorded at 0.5 kgha−1 Fe-glycine amino acid chelate by about 29.2%. The maximum increase in protein and Zn content of head rice were recorded at 1.5 kg ha−1 of glycine amino acid-Zn chelate by about 10.5 and 22.3%, respectively. The most effective treatment to enhance the Fe content of head rice was the foliar application of 1.5 kg ha−1 of glycine amino acid-Fe chelate by about 32.6%. Interestingly, the foliar application of 1 kg ha−1 Zn-glycine amino acid chelate plus 1 kg ha−1 Fe-glycine amino acid chelate at the plots that received ½ of recommended NPK increased the grain yield, and protein, Zn and Fe content of head rice compared to control by about 7%, 18.5, 7.5, and 26.2, respectively. Therefore, regardless of rates and types, foliar application of Zn and/or Fe could enhance both rice yield, protein, Zn, and Fe content of head rice both in plots that received recommended and ½ of the recommended dose of NPK.

Evaluation of safety and tolerability of iron amino acid chelate therapy in pregnant women

Background: Anemia is a major health problem. Iron deficiency is the most common cause of anemia during pregnancy. It can be associated with increased preterm labor, preeclampsia, and maternal sepsis. It can also lead to fetal loss or even perinatal deaths. The aim of the study was to monitor the oral iron therapy [Iron Amino Acid Chelate (IAAC) equivalent to elemental iron 30 mg] administered according to hospital practice and to determine the safety, and tolerability of IAAC in pregnant women.Methods: The data of pregnant women attending the outpatient department of the hospital for antenatal care between March 2020 and February 2021 and prescribed IAAC was retrospectively analyzed. It was of interest to note the changes in the Hemoglobin (Hb) levels and serum ferritin levels. These parameters were considered as the primary efficacy parameter and were analyzed by the paired t-test.Results: The data indicated very well tolerance to IAAC preparation with increase in Hb levels. After 12 week of treatment, there were significant increases in hemoglobin levels with mean rise in Hb level was 7 to 9 gm/dL. A statistically significant difference was observed at the 4th, 8th, and 12th weeks from the baseline value to each evaluation in the Hb level due to the supplementation of oral iron. The change in the serum ferritin levels was found to be statistically significant at the 12th week from the baseline values. Most of the women tolerated the oral IAAC preparationConclusions: This retrospective analysis showcased a significant improvement in the Hb and serum ferritin levels of pregnant women after 12 weeks.

Copper acetate aerosols: A possible tool complementary to vaccination in fight against SARS-CoV-2 and variants replication

In SARS-CoV-2, at the S1/S2 furin cleavage site, a four amino acid insert (P-R-R-A) not found in closely related corona viruses, has been shown to facilitate entry into respiratory epithelial cells and promote virus transmission, infectivity and virulence. By cupric aerosol treatment, complexation of these four amino acids (-P-R-R-A-), at the spike (S) protein site will lead to a conformational change possibly impeding SARS-CoV-2 replication process in the respiratory track. Since these four amino acids yield strong and stable copper complexes, subsequent to a steric hindrance, this complexation will disturb the furin-like protease cleavage at the spike protein site as it has been recently shown in vitro with copper gluconate.The compilation of stability constants for copper amino-acid complex formation, showing values of the same order of magnitude for all the twenty proteinogenic amino-acids demonstrate thermodynamically that copper amino-acid chelation for SARS-CoV-2 virus will not be affected by mutations leading to amino acid exchanges in the spike protein region. Given its low toxicity, and its very low stability formation constant, copper acetate is proposed rather than copper gluconate for possible cupric aerosol or nasal spray treatments aimed at impeding SARS-CoV-2 multiplication. It will open different medical perspectives, complementary to vaccination, in the fight against COVID 19 native virus, variants and future mutants.

Impact of amino acids on enhancing trimethyl chitosan as a nontoxic antiproliferative active biopolymer

AbstractThis work evaluates the toxicity of trimethyl chitosan (TMC), which prevents its use as an antiproliferative active compound. We report our trial to enhance its safe application by chelating with various amino acids (phenylalanine, glycine and lysine). The safety evaluation was achieved by studying the behavior of TMC–amino acid chelates against two normal human cell lines (BJ1 and RPE1), in comparison with a negative control and doxrobicin (positive control) standard samples. The results obtained show that phenylalanine and lysine play an effective role in enhancing the safety of TMC against normal cell lines, together with providing high thermal stability. Phenylalanine is superior in synthesized anticancer agents. It had IC50 values against colon HCT116 and breast cancer MCF‐7cancer cell lines of 68.7 and 51.4 μg mL–1 This compound is more acceptable than the previous reported rutin flavonoid as an anticancer agent against HCT116 cell lines. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

Influence of the Chelation Process on the Stability of Organic Trace Mineral Supplements Used in Animal Nutrition.

Simple SummaryOrganic trace minerals (OTMs) are recognised globally as being a more bioavailable source of mineral than their inorganic counterparts. Whilst there are many forms of mineral products available for use in animal nutrition, these have unfortunately been generically entitled “organic trace minerals” by virtue of the fact that the trace elements in question are complexed or otherwise associated with organic molecules. The process of chelating transition elements such as copper, iron or zinc, for instance, typically involves reacting inorganic mineral salts with suitable bonding groups, such as a peptides or amino acids, during which the mineral becomes part of a biologically stable structure. Numerous production processes have been developed, ranging from highly specific and controlled reaction processes to more involved chemical synthesis routes. The production process used determines the product’s efficacy. Given the vastly different products that exist in the marketplace, the importance of understanding the physical and chemical differences between them cannot be overstated, and as such, manufacturing processes that affect the pH stability and functionality of the finished products were assessed in this work. The results showed that in the case of proteinate type products, the hydrolysis procedure used to generate the chelating peptides had a significant impact on proteinate pH stability, a key determinant of the product’s performance in the animal.The effect of the chelation process on the pH-dependent stability of organic trace minerals (OTMs) used as mineral supplements in animal nutrition was assessed using analytical techniques such as potentiometry, Fourier Transform Infrared Spectroscopy (FTIRS) and amino acid profiling. The aim was to understand the influence and relative importance of the manufacturing conditions on mineral chelation and the subsequent pH stability of OTMs. A selection of OTMs were assessed over a wide pH range to account for the typical environmental changes encountered in the gastrointestinal (GI) tract. In the case of proteinate type products, the potentiometric assessment of free mineral concentration indicated that the hydrolysis procedure used to generate the chelating peptides was the major influencer of the pH stability of the products. Many products are available under the umbrella term “OTMs”, including amino acid complexes, amino acid chelates, polysaccharide complexes and proteinates. Significant differences in the pH-dependent stability of a range of commercially available OTMs were observed.

Design of Iridium N-Heterocyclic Carbene Amino Acid Catalysts for Asymmetric Transfer Hydrogenation of Aryl Ketones

A series of chiral complexes of the form Ir(NHC)2(aa)(H)(X) (NHC = N-heterocyclic carbene, aa = chelated amino acid, X = halide) was synthesized by oxidative addition of α-amino acids to iridium(I) bis-NHC compounds and screened for asymmetric transfer hydrogenation of ketones. Following optimization of the reaction conditions, NHC, and amino acid ligands, high enantioselectivity was achieved when employing the Ir(IMe)2(l-Pro)(H)(I) catalyst (IMe = 1,3-dimethylimidazol-2-ylidene), which asymmetrically reduces a range of acetophenone derivatives in up to 95% enantiomeric excess.

The effectiveness of foliar applications of synthesized zinc-amino acid chelates and ZnSO4 on the nutritional status of wheat plant cultivated in a soil contaminated with Cd and diesel oil

Aims: This study was done to evaluate the effectiveness of foliar applications of synthesized zinc-amino acid chelates and ZnSO4 on the nutritional status of wheat plant in a soil contaminated with Cd and diesel oil. Materials and Methods: Treatments were consist of foliar application of Zn amino acid chelate (Zn(Arg)2 and (Zn(His)2) and ZnSO4 at the rate of 0, 0.1, and 0.2% (W/V) in the soil co-contaminated with Cd (0, 10, and 20 mg Cd/kg soil) and diesel oil (0 and 8% [W/W]). Results: Application of Zn amino acid chelates had a significant effect on increasing plant nutrient status, however, soil pollution with Cd and diesel oil had an adverse effect. Based on the results of this study, application of 0.2% (W/V) (Zn(Arg)2 and (Zn(His)2) significantly increased the grain Zn concentration of the plants grown in the soil polluted with Cd (10 mg Cd/kg soil) and diesel oil (8% [W/W]) by 11.3% and 10.1%, respectively. For co-contaminated soil with Cd and diesel oil, it was increased by 9.8%. Soil microbial respiration has affected by Zn amino acid chelate and soil pollution. According to our results, application of 0.2% (W/V) Zn(Arg)2 chelate significantly increased the soil microbial respiration in the soil polluted with Cd (10 mg Cd/kg soil) and diesel oil (8% [W/W]) by 12.5% and 13.1%, respectively. Conclusion: Zn amino acid chelate had a significant effect in increasing plant nutrient status such as Zn and Fe that is a positive point environmental study.

Use of Calcium Amino Acid Chelate in the Production of Acid-Curd Goat Cheese.

Amino acid chelates are a new group of compounds approved for food enrichment, however there is no previous research using calcium amino acid chelate to enrich goat’s milk products. The purpose of this research was to evaluate the possibility of using calcium amino acid chelate to produce goat’s acid-curd cheese. In this study, four types of acid-curd cheeses from goat’s milk subjected to 85 °C/5 min treatment were produced: control cheeses—made from milk without calcium addition and cheeses from milk enriched with 30, 35 and 40 mg of Ca (in 100 g of milk) in the form of calcium amino acid chelate. Goat cheese with calcium amino acid chelate had a higher moisture content, and a lower fat content. More fat was separated with the whey. In cheeses made from the milk with calcium amino acid chelate there was no goaty taste. Enrichment with 35 mg of Ca in 100 g of goat milk increased the calcium content in cheese by 60.5% in comparison to the control sample. However, the enrichment of goat milk with 40 mg Ca (in 100 g of processed milk) increased the calcium content in cheese by only 63.29%.

EFFICIENT SYNTHESIS OF NOVEL TRICYCLIC BENZOXAZINE DERIVATIVE VIA RING OPENING OF EPOXIDE ALONG THE MP AND DFT STUDIES OF STRUCTURAL, SPECTROSCOPIC (IR, RAMAN, UV-VIS), THERMODYNAMIC, ORBITALS AND NLO PROPERTIES OF DESIRED TRICYCLIC BENZOXAZINE DERIVATIVE

Ring opening of 2-nitro phenyl glycidyl ether by chelated amino acid ester enolate provides access to desired novel benzoxazine derivative just over a few steps. Theoretical study on the molecular structure of 2,2,2-trifluoro-N-(1-oxo-2,3,3a,4-tetrahydro-1H-benzo[b]pyrrolo[1,2-d][1,4]ox-azin-2-yl) acetamide (S11) is presented by using second order Moller Plesset (MP2) as well as density functional theory (DFT) level calculations. The calculated vibrational frequencies were assigned into normal modes of vibration by the use of potential energy distribution (PED). The positive charge on all hydrogen atoms were obtained by charge distribution calculations using Mulliken, electrostatic and natural charge distributions. Similar electrophilic and nucleophilic regions were observed from the calculated electrostatic potential surface calculations. The time dependent density functional theory (TD-DFT) calculations were performed to obtain electronic transitions within the molecule. The frontier molecular orbital (FMO) analysis was leading to the possible charge transfer within the molecule. The natural bond orbital (NBO) analysis provided information regarding the interaction between the donor and acceptor in bond. The statistical thermodynamic functions (dipole moment, internal energy, enthalpy, Gibbs free energy, entropy, heat capacities and partition functions) were calculated at the range of temperature from 10-500 K

The effectiveness of seed priming and foliar application of zinc- amino acid chelates in comparison with zinc sulfate on yield and grain nutritional quality of common bean

The efficacy of seed priming and foliar application of zinc-amino acid chelates including zinc-histidine [Zn(His)2] and zinc-methionine [Zn(Met)2] in comparison with zinc sulfate (ZnSO4) on yield and grain nutritional quality of two common bean cultivars (Phaseolus vulgaris L., cvs Talash and Sadri) was investigated in a severely Zn-deficient calcareous soil (DTPA-Zn: 0.38 mg kg−1 soil) in a pot experiment. Bean response to Zn application varied depending on the Zn fertilizer, application method and cultivar. In ‘Talash’, seed priming with [Zn(His)2] and [Zn(Met)2] led to 24.1 and 11.6% increase in the grain yield of bean in comparison with ZnSO4 treatment, respectively. In both cultivars, foliar application of [Zn(His)2] led to significant increase in the grain yield in comparison with ZnSO4. The highest grain Zn concentration was obtained by seed priming with [Zn(Met)2] in ‘Sadri’ and [Zn(His)2] in ‘Talash’, respectively. For Zn-amino acid chelates, seed priming was more effective than foliar application in increasing grain yield and Zn concentration. Foliar application of [Zn(His)2] and [Zn(Met)2] in ‘Sadri’ and [Zn(Met)2] in ‘Talash’ resulted in higher protein content in bean grain as compared with ZnSO4. In both cultivars, foliar application of [Zn(Met)2] was the more effective than seed priming to increase grain protein content. The highest water-soluble carbohydrates concentration of grain was obtained by seed priming with [Zn(Met)2] and [Zn(His)2] in ‘Sadri’ and ‘Talash’ cultivars, respectively. Therefore, seed priming with [Zn(His)2] and ZnSO4 in ‘Sadri’ and [Zn(Met)2] in ‘Talash’ can effectively be used for improving yield of common bean in Zn-deficient calcareous soils.

Submicromolar copper (II) ions stimulate transretinal signaling in the isolated retina from wild type but not from Cav2.3-deficient mice

BackgroundSo far, only indirect evidence exists for the pharmacoresistant R-type voltage-gated Ca2+ channel (VGCC) to be involved in transretinal signaling by triggering GABA-release onto ON-bipolar neurons. This release of inhibitory neurotransmitters was deduced from the sensitivity of the b-wave to stimulation by Ni2+, Zn2+ and Cu2+. To further confirm the interpretation of these findings, we compared the effects of Cu2+ application and chelation (using kainic acid, KA) on the neural retina from wildtype and Cav2.3-deficient mice. Furthermore, the immediately effect of KA on the ERG b-wave modulation was assessed.MethodsTransretinal signaling was recorded as an ERG from the superfused murine retina isolated from wildtype and Cav2.3-deficient mice.ResultsIn mice, the stimulating effect of 100 nM CuCl2 is absent in the retinae from Cav2.3-deficient mice, but prominent in Cav2.3-competent mice. Application of up to 3 mM tricine does not affect the murine b-wave in both genotypes, most likely because of chelating amino acids present in the murine nutrient solution. Application of 27 μM KA significantly increased the b-wave amplitude in wild type and Cav2.3 (−|-) mice. This effect can most likely be explained by the stimulation of endogenous KA-receptors described in horizontal, OFF-bipolar, amacrine or ganglion cells, which could not be fully blocked in the present study.ConclusionCu2+-dependent modulation of transretinal signaling only occurs in the murine retina from Cav2.3 competent mice, supporting the ideas derived from previous work in the bovine retina that R-type Ca2+ channels are involved in shaping transretinal responses during light perception.

Evaluation of chelated iron and iron sulfate in the diet of African catfish, Clarias gariepinus to enhance iron excretion for application in integrated aquaponics systems

AbstractThe best source and inclusion level of iron from feed additives for optimizing the production of African catfish was determined while simultaneously evaluating excretion of effluent that can be used to optimally grow plants in aquaponics systems. Using six experimental treatments and a control, the production parameters, proximate composition, hematological indices, nonspecific immunity, tissue mineral composition, apparent digestibility coefficients, and water quality trials were examined. Mineral sources were iron from an organic source, chelated amino acid, and an inorganic source, iron phosphate. No significant differences were observed in production parameters. There were differences in proximate composition. Fish fed FeSO4 at 30 mg/kg had significantly higher hematocrit levels and red blood cell levels compared with the control, while FeSO4 60 had the highest hemoglobin levels. Tissue mineral composition showed significantly higher iron concentrations in the liver of the control compared with all the treatments. FeSO4 30 treatment had the highest apparent digestibility coefficient (96%). Iron concentrations in the effluent of the water differed between treatments. Iron from FeSO4 can improve the hematological profile of catfish compared with the control, and its effluent from culture water has the potential to minimize or reduce the use of nutrient fertilizers in integrated aquaponics systems.

Fortification of Bread Wheat Using Synthesized Zn-Glycine and Zn-Alanine Chelates in Comparison with ZnSO4 in a Calcareous Soil

ABSTRACT Calcareous soils typically suffer from zinc deficiency and zinc sulfate is incorporated in many cultivated soils. Utilization of ZnSO4 has some kinds of interaction with soil particles and organic matter. In this study, the efficacy of two znic(Zn)-amino acid chelates (Zn-ACs) i.e., Zn-alanine (Zn-Ala) and Zn-glycine (Zn-Gly) on wheat (Triticum aestivum, cv. N91-8) growth characteristics and zinc concentration in wheat was examined under greenhouse conditions and compared to the a commercial ZnSO4. Results showed that Zn-Ala and Zn-Gly significantly increased the dry weight and shoot length of wheat in comparison to ZnSO4 treatment. Soil application of Zn-Amino acid chelates proved to be the most influential source of zinc in increasing wheat growth and yield indices. Number of fertile spikelet and grain yield increased significantly respectively compared to ZnSO4 treatment. Zn concentration and protein content of wheat grain in Zn-ACs treatment was significantly higher than the ZnSO4 treatment. Soil application of Zn-ACs caused a significant decrease in the grain phytic acid (PA) concentration and also phytic acid to zinc molar ratio in comparison with ZnSO4 treatment. According to the results, Zn-ACs could be utilized as a zinc fertilizer source for improving the zinc bioavailability in wheat.

Amino Acid Chelated Iron versus Ferrous Fumarate in the Treatment of Iron Deficiency Anemia with Pregnancy : Randomized Controlled Trial

Background: Anemia is a major health problem. Iron deficiency is the most common cause of anemia during pregnancy. Suboptimal iron content in the average mother’s diet and the presence of insufficient iron stores during the reproductive years are causes for this predominance. In many developing countries, iron deficiency anemia (IDA) in pregnancy is more of a rule than an exception with a prevalence of 52%. In the Western societies, the frequency of IDA is approximately 25% in pregnant women not taking iron supplements and less than 5% in women taking iron supplements. Anemia has a significant impact on the health of the fetus and the mother. It can be associated with increased preterm labour, preeclampsia, and maternal sepsis. It can also lead to fetal loss or even perinatal deaths.Aim: To find out whether there is a difference between amino acid chelated iron and iron salts (ferrous fumarate) in effect, safety, adverse effects and outcomes in treatment of iron deficiency anemia during pregnancy.Patients and Methods: The study was conducted on pregnant women attending the antenatal care in the outpatient clinic at Ain-Shams University Maternity Hospital in the period from February 2019 to July 2019, with diagnosis of iron deficiency anemia between 14 -18 weeks with hemoglobin (HB) level 8–10.5g/dL, and serum ferritin <15μg/l. One hundred fifty pregnant women who met the criteria were randomized in to 2 groups (iron chelated amino acid “IAAC” group and ferrous fumarate “FF” group). Hemoglobin level, blood indices, serum iron and serum ferritin levels were measured in both groups at 4, 8, 12 weeks of treatment.Results: There was a significant hematological improvement (mean HB level, blood indices, serum iron and ferritin levels) in both groups which was significantly higher in IAAC group.Conclusion: Iron amino acid chelate achieves faster cure rate than ferrous fumarate in women with iron deficiency anemia in pregnancy as regards mean HB and serum ferritin level. Iron amino acid chelate is associated with fewer side effects than ferrous fumarate.