Aiming at the problem of source-load uncertainty caused by the increasing penetration of renewable energy and the large-scale integration of electric vehicles (EVs) into modern power system, a robust optimal operation scheduling algorithm for regional integrated energy systems (RIESs) with such uncertain situations is urgently needed. Based on this background, aiming at the problem of the irregular charging demand of EV, this paper first proposes an EV charging demand model based on the trip chain theory. Secondly, a multi-RIES optimization operation model including a shared energy storage station (SESS) and integrated demand response (IDR) is established. Aiming at the uncertainty problem of renewable energy, this paper transforms this kind of problem into a dynamic robust optimization with time-varying parameters and proposes an improved robust optimization over time (ROOT) algorithm based on the scenario method and establishes an optimal scheduling mode with the minimum daily operation cost of a multi-regional integrated energy system. Finally, the proposed uncertainty analysis method is verified by an example of multi-RIES. The simulation results show that in the case of the improved ROOT proposed in this paper to solve the robust solution of renewable energy, compared with the traditional charging load demand that regards the EVs as a whole, the EV charging load demand based on the trip chain can reduce the cost of EV charging by 3.5% and the operating cost of the multi-RIES by 11.7%. With the increasing number of EVs, the choice of the starting point of the future EV trip chain is more variable, and the choice of charging methods is more abundant. Therefore, modeling the charging demand of EVs under more complex trip chains is the work that needs to be studied in the future.