Identification Of Characteristics Research Articles

Blow flies (Diptera: Calliphoridae) of the Baja California Peninsula

The blow fly fauna of the Baja California peninsula, Mexico has been sparsely documented. This study incorporates and lists recent and historical records of blow flies from collecting trips throughout the peninsula, visits to major southern California museums for historical collection records, and a literature search. Seven genera and 16 species are reported from the two peninsular Mexican States, Baja California, and Baja California Sur. Three species are recorded that are not included in the 2020 Catalog of Mexican Calliphoridae by Jaume-Schinkel, & Ibanez-Bernal (2020), they are: Calliphora livida, Calliphora terraenovae, and Calliphora vomitoria. One blow fly species, Cochliomyia hominivorax (the primary Screw-Worm Fly) is not included here but is found in museum collections. Once present in Baja, it is considered eradicated from continental North America as far south as the Panama Canal by the U.S. Department of Agriculture (USDA) however, it is occasionally reintroduced (Skoda et al. 2018) and is still present in some Caribbean islands. It is noteworthy that all calliphorid species from this mountainous desert peninsula are also found in the State of California, USA which supports 11 genera and 47 species in this family (Whitworth 2006) primarily because of its more varied climate and terrain. The Baja peninsula can be considered a southern extension of the State of California, with reduced species representation due to reduced climate and terrain variation, so no separate identification keys are necessary. Forensic entomologists and taxonomists can use the blow fly keys for North America by Whitworth, 2006, and 2017 for this region. The latest key by Jones, Whitworth, and Marshall (2019) is revolutionary and much welcome in taxonomy in that it is an online computer-aided key that has actual macro-photos of species identification characters and is intended to be used with a computer screen next to your microscope for identifications in place of printed material.

Pharmacognostic Study and Standardization of Leaves and Stem of Mentha spicata Linn.

Background:: Various Mentha species are cultivated all over the world due to their aroma and valuable therapeutic constituents. Along with its traditional use as a food flavor, it is well-established folklore medicine for treating digestive and cardiovascular problems. Mentha spicata L. is a perennial, aromatic herb belonging to the Lamiaceae family. M. spicata leaves have been used in traditional systems of medicines for treating various digestive system disorders since old times. Aim:: The present research work encompasses the pharmacognostic study, phytochemical screening and physicochemical parameter estimation of leaves and stem of M. spicata L. for standardization of Mentha plant. Materials and Methods:: Morpho-anatomical characterization, physicochemical analysis and preliminary phytochemical investigation of leaves and stems were performed. These investigations were done as per WHO guidelines to determine the main diagnostic characteristics for correct identification and standardization of whole plant and powdered drug of M. spicata L. Results:: The dimensions, size, color, and surface texture characteristics of fresh crude drug (leaf and stem) were determined. Compound microscope images of the transverse section (TS) of M. spicata leaf and stem revealed the cellular arrangement and main identification characters. Phytochemical and physicochemical analysis of powdered drugs confirmed the presence of important phytoconstituents such as phytosterol, terpeneoids, phenolic compounds, saponins, volatile oil, etc. Conclusion:: The results obtained are helpful for the characterization of the crude drug. Morphoanatomical characterization and physicochemical analysis are inexpensive methods and serve as a quality control tool for stem and leaf standardization. The ethanolic and aqueous extract of leaves and stem showed the presence of valuable medicinally active phytoconstituents.

Identification of molecular characters of tomato seedlings (Solanum lycopersicum) from Limpopo Province, South Africa, using 28S rDNA

Farming with tomatoes (Solanum lycopersicum) in South Africa can be a highly profitable operation, provided the farmer starts the crop with the right variety of quality seedlings, suited for the specific growth area. Therefore, it is necessary to study tomato seedlings on the molecular level. This study was conducted to isolate and identify S. lycopersicum in Limpopo Province, South Africa. Therefore, this molecular study was conducted in 2022 at Limpopo University. To identify S. lycopersicum 28S rDNA marker was used. The DNA was extracted using the Chelex method. The tomato was identified as S. lycopersicum. The Nblast analysis showed South African S. lycopersicum has a 99.8% similarity with a population from Germany (OU640345) and a 99.7% similarity with a population from Sweden (OK073662). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as S. lycopersicum in the same clade with highly supported (83) bootstrap values. In conclusion, this species is identified using 28S rDNA properly. However, other DNA markers are recommended to understand Solanum spp. phylogeny better.

Plant Taxonomy - Its Past Role and Future Lines of Action in India

The history of floristic and taxonomic research in India is briefly reviewed and the decline and revival of floristic studies in India is traced.Modern concepts in plant taxonomy are towards the synthesis of orthodox taxonomic practices and modern methodologies. There need not be any clash between the so called alpha and omega taxonomists. All data that can be readily scanned for the species is useful for the taxonomist. The only aspect that has to be considered is the utillty of classification for a specified purpose. A field, or forest botanist needs diagnostic keys based on superficial morphological characters for identification of plants in the field. Characters based on anatomy, embryology chromosome structure and number, palynology, plant alkaloids and protein serum analysis are useful tools for finding out the systematic position of plants of doubtful affinity; however,. these characters cannot be readily used in the field for identification of plants. The herbarium identification of plants is the simple method of retrieval of plant identity through standard specimens properly authenticated by an expert. It does not give legality for the concept of fixity of species as wrongly alluded to by some non-taxonomists, since Nomenclatural Type is a constituent element of a taxon and it is not necessarily the most representative element.Some of the lines where taxonomists could play a major role are the inventory listing of plants for the conservation of species, monitoring rate of decline and spread of species, establishment of botanical record centres, environmental education and collaboration with universities for reorientation of teaching methods.The necessity for floristic training is stressed and a syllabus drawn up.

Tenuibiotus yeliseii sp. nov., a new species of Macrobiotidae (Tardigrada: Eutardigrada) from Svalbard, Norway, with discussion of taxonomic criteria within the genus and its phylogeny

We describe Tenuibiotus yeliseii sp. nov., a new tardigrade species from Svalbard, using morphological and morphometric analyses conducted with the use of light and scanning electron microscopy, as well as genetic analyses based on four molecular markers (three nuclear, 18S rRNA, 28S rRNA, ITS-2, and one mitochondrial, COI). A phylogenetic analysis of the genus Tenuibiotus Pilato et Lisi, 2011 is conducted using new data. In addition, the taxonomic significance of gibbosities on leg IV as a key character for species identification in Tenuibiotus is discussed. A key to the species of Tenuibiotus is proposed.

Anatomical features of ecological importance and taxonomic value revealed by scanning electron microscopy and light microscopy in Camonea umbellata (L.) A.R. Simões & Staples (Convolvulaceae).

This article describes detailed and novel data on the anatomy and histochemistry of leaves, stems, and roots of Camonea umbellata (L.) A.R.Simões & Staples in different environments for the identification of characters with taxonomical value and of ecological importance, with provision of light and scanning electron microscopy images. To analyze the characters, we collected samples of the vegetative organs of three individuals in each of three populations, which were in a grazing area, an urban environment, and a biological reserve. The main diagnostic anatomical markers for the identification of C. umbellata include amphistomatic leaves, tetracytic and brachyparatetracytic stomata, peltate trichomes, long simple trichomes, epidermis with striated cuticle ornamentation, mesophyll with acute borders, presence of druses, secretory channels, angular collenchyma, fibrous pericycle in the stem, intraxylary phloem in the vegetative organs, oil bodies throughout the midrib, petiole, stem and root, and epicuticular waxes of the crust and coiled rodlet types. Since the characters above did not show variation in the environments evaluated, we consider these characters taxonomically useful for the identification of C. umbellata. RESEARCH HIGHLIGHTS: The anatomy of the aerial vegetative organs of Camonnea umbellata retains common Convolvulaceae characters. The sinuosity of the epidermal cell walls and the density of trichomes in the epidermis of the petiole were visually variable characters among the analyzed individuals. Amphistomatic leaves, tetracytic and brachyparatetracytic stomata, peltate trichomes, epidermis with striated cuticle ornamentation, dorsiventral mesophyll with border acute, presence of druses, secretory structures, angular collenchyma, fibrous pericycle in the stem, intraxillary phloem, presence of oil bodies in all organs, and epicuticular waxes of the crust type and coiled rods were considered important anatomical markers for the recognition and correct identification of Camonea umbellata.

Microscopy, HPTLC, and LC-DAD-Q-ToF validation of nut-based weight-loss dietary supplements, Aleurites moluccanus (candlenut) and Bertholletia excelsa (Brazil nut)

Aleurites moluccanus (candlenut) and Bertholletia excelsa (Brazil nut) are marketed as dietary supplements for weight loss. These dietary supplements have been found to sometimes be adulterated with toxic nuts/seeds from Cascabela thevetia, commonly known as yellow oleander or lucky nut. This study emphasizes the key identification parameters to differentiate the genuine and adulterated nuts. Samples were obtained from authenticated sources of the nuts and from commercial sources of dietary supplements. This study examined 38 samples, including voucher and commercial samples. All eight commercial candlenut dietary supplement samples were adulterated. Additionally, two samples sold as Brazil nuts were also found to be adulterated. Other nuts were screened for the presence of Cardiac Glycosides, but none were found to be positive. The presence of yellow oleander was confirmed in all commercial dietary supplement samples marketed as candlenut as well as in commercial samples of Brazil nut. This study provides simple key identification characters using micro-morphology and histochemical localization of cardiac glycosides in the commercial nuts, HPTLC fingerprints, and LC-DAD-Q-ToF analytical parameters to detect and identify adulteration in commercial products.

Phylogenetic evidence of a possible Trichuris globulosa species complex in Arabian camels from Kuwait.

During a 1-year study, Trichuris adults were obtained after necropsy of Arabian camels (Camelus dromedarius) from a slaughterhouse in Kuwait. Morphological and molecular identification was performed to confirm the identity of the Trichuris specimens obtained from C. dromedarius. Fifteen male Trichuris specimens were selected, and molecular identification was performed using mitochondrial cytochrome c oxidase subunit I, 12S ribosomal RNA, 16S ribosomal RNA genes and the nuclear internal transcribed spacer 2 (ITS2) region. Through phylogenetic analysis, 2 distinct groups were obtained using the mitochondrial genes, where group 1 showed a close relationship to Trichuris globulosa while group 2 showed a close relationship to Trichuris ovis, providing molecular evidence of a possible T. globulosa species complex. Additionally, the nuclear ITS2 region did not provide enough resolution to distinguish between the 2 groups of Trichuris specimens. Observation of morphological characters revealed variations in the shape of the male spicule sheath, where specimens present either a globular posteriorly truncated swelling or the absence of posteriorly truncated swelling. Moreover, the variations in male spicule sheath does not corroborate with the results of molecular data, suggesting the limited use of this character for identification of T. globulosa. In conclusion, molecular analysis suggests a possible species complex in T. globulosa, with the mitochondrial genetic markers successfully differentiating between the 2 groups. The limited use of the male spicule sheath as a diagnostic character for identification of T. globulosa is suggested.

Violins we see, species we don’t… Species delimitation of the spider genus Loxosceles Heineken & Lowe (Araneae: Sicariidae) from North America using morphological and molecular evidence

In modern systematics, different sources of evidence are commonly used for the discovery, identification, and delimitation of species, especially when morphology fails to delineate between species or in underestimated species complexes or cryptic species. In this study, morphological data and two DNA barcoding markers—cytochrome c oxidase subunit I (COI) and internal transcribed spacer 2 (ITS2)—were used to delimit species in the spider genus Loxosceles from North America. The molecular species delimitation analyses were carried out using three different methods under the corrected p-distance Neighbor-Joining (NJ) criteria: 1) Assemble Species by Automatic Partitioning (ASAP), 2) General Mixed Yule Coalescent model (GMYC), and 3) Bayesian Poisson Tree Processes (bPTP). The analyses incorporated 192 terminals corresponding to 43 putative species of Loxosceles, of which 15 are newly recognized herein, as putative new species, based on morphology and congruence between molecular methods with COI. The average intraspecific genetic distance (p-distance) was <2%, whereas the average interspecific genetic distance was 15.6%. The GMYC and bPTP molecular methods recovered 65-79 and 69 species respectively, overestimating the diversity in comparison with morphology, whereas the ASAP method delimited 60 species. The morphology of primary sexual structures (males palps and female seminal receptacles) was congruent with most of the molecular methods mainly with COI, showing that they are robust characters for identification at the species level. For species delimitation COI was more informative than ITS2. The diversity of Loxosceles species is still underestimated for North America, particularly in Mexico which holds the highest diversity of this genus worldwide.

Revision of the genus Robbea (Stilbonematinae: Desmodoridae), worldwide abundant marine nematodes with chromophoric Fe–Br inclusions and the description of a new stilbonematine genus

Abstract Nematodes are one of the most widespread and abundant animal taxa across aquatic and terrestrial environments. In marine shallow-water, porous sediments, members of the subfamily Stilbonematinae may be found in high numbers. Stilbonematinae are characterized by their coat of symbiotic bacteria, which give the nematodes a white appearance, while the nematodes themselves are usually colourless. We identified several species of the genus Robbea (Desmodoridae: Stilbonematinae) in which live specimens had a conspicuous dark purple coloration of the glandular sense organs (GSOs), highly specialized epidermal glands that are prominent in the Stilbonematinae. The dark inclusions in the GSOs of Robbea contained high concentrations of iron and bromide, regardless of their habitat. Morphological and phylogenetic analyses show that the genus Robbea is paraphyletic and we define the new genus Cyathorobbea gen. nov. for species with sucker-shaped post-pharyngeal supplements in males. Furthermore, we describe five new Robbea species from around the world. Some Robbea species are morphologically very similar and required the use of principal component analysis of morphometric data to untangle the diversity in this taxon. Our work emphasizes the value of live animal observations that enable the identification of important characters and highlights the overlooked diversity within the Stilbonematinae.

Lesbian Lives in Soviet Latvia: The Narratives

This article employs narrative analysis to examine three testimonies provided by individuals who were contemporaries of lesbians in Soviet Latvia. The author’s research is based on two oral history interviews and the diary entries of a homosexual man, dating between the 1950s and 1980s. The research was carried out in four stages: (1) story analysis; (2) identification of main topics and characters; (3) identification of main terms and tone; and (4) extraction of three key narratives. The findings demonstrate, firstly, the lack of terminology to describe homosexual/non-heterosexual relationships between women when referencing the Soviet time. This indicates that contemporary terms used by the LGBTQI+ community may not be valid for analysing the experiences of non-heterosexual people in Soviet Latvia. Secondly, the testimonies described in this article view same-sex women’s relationships through heteronormative lens as deviating from the norm. Thirdly, the article highlights how rigid expectations of “manly” and “womanly” behaviour and traits are used to judge compliance with or deviation from societal norms. The understanding of non-heterosexual relationships between women, as evidenced by the sources, cannot be standardised. While the testimonies reference non-heterosexual relationships between women, they also mention same-sex relationships, which may have formed due to socioeconomic conditions and different intimate bonds.

Qin Seal Script Character Recognition with Fuzzy and Incomplete Information

The dependable and efficient identification of Qin seal script characters is pivotal in the discovery, preservation, and inheritance of the distinctive cultural values embodied by these artifacts. This paper uses image histograms of oriented gradients (HOG) features and an SVM model to discuss a character recognition model for identifying partial and blurred Qin seal script characters. The model achieves accurate recognition on a small, imbalanced dataset. Firstly, a dataset of Qin seal script image samples is established, and Gaussian filtering is employed to remove image noise. Subsequently, the gamma transformation algorithm adjusts the image brightness and enhances the contrast between font structures and image backgrounds. After a series of preprocessing operations, the oriented gradient histograms (HOG) features are extracted from the images. During model training, different weights are assigned to classes with varying sample quantities to address the issue of class imbalance and improve the model's classification accuracy. Results show that the model achieves an accuracy of 95.30%. This research can help historians quickly identify and extract the text content on newly discovered Qin slip cultural relics, shortening the cycle of building a historical database.

Taxonomic investigation of monilophytes from North Waziristan Tribal District KP, Pakistan

Floristic surveys and assessment of relationships between species richness and elevational gradients help in developing more efficient conserving strategies for biodiversity under climate change. North Waziristan Tribal District (NWD) has been identified as the second largest district in FATA. The study area encompasses diverse altitudes, ranging from 600 m to over 3000 m. Due to its distinct geological history, low temperature, and rich biodiversity, the North Waziristan Tribal District gives an excellent opportunity to assess taxonomic distribution of ferns. Field visits were conducted during 2021, thoroughly documenting fern species along with essential field-related characteristics such as habits, habitats, GPS coordinates, etc. Floristic studies of ferns (monilophytes) from NWD showed that it has an exceptionally diverse monilophyte flora. A total of 114 fern specimens were collected during these field visits. After a comprehensive examination, 17 species distributed across 13 genera and 7 families were identified. The predominant family was Pteridaceae, comprising six species, followed by Aspleniaceae and Thelypteridaceae with three species each. Additionally, Dryopteridaceae accounted for two species, while the remaining three had only one species each. Asplenium was the leading genus, documented with three species, followed by Pteris, Cheilanthes, and Adiantum, each with two species. Finally, a dichotomous key was made based on morphological, anatomical, and palynological characters for identification and characterization purposes. In terms of altitude, the highest number of taxa were observed at elevations above 2200 m, while the lowest species count was reported between 600 -1000 m. Species richness of ferns reached to a maximum at moderate elevations, which could have been due to combination of moderately low temperature and high humidity. Ethnobotanical documentation revealed eight species, six genera, and four families within the study area.

A review on identification characters and IPM of wheat aphid by using non-conventional methods

A review on identification characters and IPM of wheat aphid by using non-conventional methods

Characterization of anatomical characters of Peperomia (Piperaceae) from Asia for taxonomy

Abstract The flowers of Peperomia (Piperaceae) are simple, minute, and unique structures within the angiosperms consisting of a rounded bract, two stamens and an ovary, but generally lack taxonomic value at species level. Because of this, morphological characters of vegetative parts have been mainly used for species level taxonomy. There is a need to supplement these vegetative characters with anatomical characters. We selected 17 native Thai species representatives of South East Asian Peperomia to investigate the utility of vegetative anatomical characters for species delimitation, taxon identification, and classification. Stem, petiole, and leaf anatomical characters were studied using standard microscopic techniques. The anatomical characters demonstrated high diversity and supported species identification. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) culstering, Principal Coordinates analysis (PCoA), and Principal Component Anaysis (PCA) were used to investigate patterns in the multivariate anatomical data and identify groupings of value to species classification. Four broad groupings were retrieved and some subgroupings identified. The most important characters that differentiated the species in the multivariate analysis included stem shape (roundness and ridges), petiole indumentum and trichome type, vascular bundle pattern and number, stem pith cavity, mucilage canals, and stem cortex sclerenchyma. These data were further used to prepare anatomical descriptions at generic and species levels and to provide a key to species.

RDNA and mtDNA analysis for the identification of genetic characters in the hybrid grouper derived from hybridization of Cromileptes altivelis (female) × Epinephelus lanceolatus (male)

BackgroundHybridization is a useful strategy to produce offspring with more desirable phenotypic characteristics than those of parents. The hybrid grouper derived from the cross of Cromileptes altivelis (♀, 2n = 48) with Epinephelus lanceolatus (♂, 2n = 48) exhibits improved growth compared with its female parent, which makes it valuable to aquaculture. However, the genetic traits of the hybrid grouper are poorly understood.ResultsThe observations showed that the hybrid grouper was diploid (2n = 48) and displayed intermediate morphology with the parent's measurable characteristics. The ribosomal DNA (rDNA) and mitochondria DNA (mtDNA) were characterized at molecular and phylogenetic level. High similarity and low genetic distance of 5S rDNA and mtDNA sequences between the hybrid grouper and C. altivelis showed that the hybrid grouper had a closer genetic relationship with female parents. The reconstructed phylogenetic tree based on COI gene and D-loop region of mtDNA recovered that mtDNA was maternally inherited in the hybrid grouper. Additionally, the DNA methylation level of 5S rDNA intergenic spacers (IGS) sequence was tested in here. The results showed that the DNA methylation status of the hybrid grouper was significantly lower than that of C. altivelis.ConclusionResults of this study provide important data on the genetic characteristics of the hybrid derived from the cross of C. altivelis and E. lanceolatus, and contribute the knowledge of both evolution and marine fish breeding.

Analysis of Five Topics in Multi-Scale Identification of Landscape Characters in Europe

Analysis of Five Topics in Multi-Scale Identification of Landscape Characters in Europe

Identification of Characters of Cultural Landscape in Wuyishan National Park from the Perspective of Stratification

Identification of Characters of Cultural Landscape in Wuyishan National Park from the Perspective of Stratification

Identification and Morphological Characterization of Sugar Palm Plants (Arenga pinnata Merr.) Growing on Different Altitudes

Trees of sugar palm (Arenga pinnata Merr.) can grow at an altitude of 0-1400 m above sea level. The best growth of the trees is at an altitude of 500-700 m above sea level. Sugar palm trees also have a wide adaptability to various environmental conditions. The purpose of this research was to determine the morphological characteristicss of sugar palm plants grown at an altitude of 500, 650, and 900 m above sea level so that the kinship relationship can be known. This research was conducted at three altitudes in Lareh Sago Halaban District, Lima Puluh Kota Regency, West Sumatra, namely Nagari Labuah Gunung (altitude 900 m above sea level), Nagari Bukik Sikumpa (altitude 650 m above sea level), and Nagari Tanjung Gadang (altitude 500 m above sea level) from July to August 2023. The method used was a survey with a purposive sampling technique. Identification was carried out on 36 morphological characters, including stems, leaves, fibers, fruits, and seeds. The data obtained were analyzed for their diversity and similarity. The results of the identification and characterization of qualitative and quantitative morphological characters at three different altitudes showed a relatively narrow diversity value. The overall morphological characters of the sugar palm plants were divided into 2 large groups with a coefficient value of 0.72 or 72% similarity distance.

Revealing the intricate microcosm: Advancing invasive species pollen analysis through scanning microscopy of ultra sculpture.

Micromorphological visualization of plant surface peculiarities provides valuable characters for the precise identification of plant species. Invasive alien species, introduced outside their native range, pose significant ecological, and health challenges. This study focuses on micromorphological investigations of selected invasive plant species belonging to the families Amaranthaceae, Asteraceae, Moraceae, Crassulaceae, Cannabaceae, Fabaceae, Commelinaceae, and Oxalidaceae. The study employs scanning electron microscopy (SEM) to analyze the species micromorphology of pollen structure in depth to characterize the sculpturing patterns. Additionally, the study examines the pollen characteristics of these invasive plants, including shape, size, and fertility, along with exine sculpturing. The maximum polar diameter was observed for Senna tora (41.2 μm). The spines in Achillea fragrantissima were measured to have dimensions of approximately 1.91 μm in length and 2.11 μm in width. The findings shed light on the allergy-causing potential of these invasive species, providing crucial information for accurate identification and effective management strategies to safeguard the indigenous flora of the region. The study contributes to the knowledge base for botanists, taxonomists, policy makers, climate experts, and biodiversity specialists. RESEARCH HIGHLIGHTS: Integrating SEM to analyze the pollen of invasive species. Unveiling pollen micromorphology of invasive species. Exploring the microscopic realm of invasive pollen to accurately identify the species.