Characterization Of Nanocrystalline Cellulose Research Articles

NANO-CRYSTALLINE CELLULOSE OF AFRICAN BEAN (PENTACLETHRA MACROPHYLLA BENTH) POD: EXTRACTION AND CHARACTERISATION

Nanocrystalline cellulose is a unique nanomaterial derived from the most abundant and almost inexhaustible natural polymer, cellulose. It is considered a sustainable nanomaterial due to its availability, biodegradability, and biocompatibility. Based on its desired structural, thermal, and morphological characteristics, it is suitable for mechanical and industrial applications. The research reports on the extraction and characterization of nanocrystalline cellulose from African bean (PentaclethramacrophyllaBenth) pods. In this paper, nano-crystalline cellulose (CNCs) were isolated from the cellulose extracted from Africa oil bean pod waste, followed by characterization using XRD, FT-IR, TGA, DGA, and SEM to determine its crystallinity, structural properties, thermal characteristics, and morphology, respectively. The result revealed that the Africa bean pod amorphous segments containing hemicelluloses and lignin were extensively removed with increasing chemical treatments, leading to increased purity, crystallinity index, and thermal stability of the extracted materials. The crystallinity index of nanocrystalline cellulose was estimated at 71.43%, which is better than both raw oil bean powder and isolated cellulose. This therefore implies that the nanocrystalline cellulose of the oil bean pod can serve different mechanical and structural applications.

Extraction and Characterization of Nanocrystalline Cellulose from Sugar Palm Fibres (Arenga Pinnata)

In this work, cellulose was isolated from sugar palm fibre (Arenga pinnata) and converted into sugar palm fibre nanocrystalline cellulose (NCCs) in the hamlet of Medan Sinembah, Indonesia. Alkalization and bleaching were the stages of the therapy process. The cellulose removed with 30 weight percent concentrated sulfuric acid was then used to isolate NCC. Using Fourier transform infrared spectroscopy (FT-IR), the chemical make-up of sugar palm fibre and NCCs was identified. On the basis of structural analysis, X-ray diffraction (XRD) was used. Scanning electron microscopy (SEM) was used for morphological examination, while transmission electron microscopy (TEM) was used to look into NCCs. The outcomes demonstrated that the extracted cellulose underwent bleaching and alkalization processes, respectively, to eliminate lignin and hemicellulose. The diameters of the sugar palm fibre and NCCs were determined 69.025 µm to 6.07±0.14 nm and, respectively

"Extraction and Characterization of Nanocrystalline Cellulose from Agricultural Residue-Oil Palm Petiole Fiber "

"Extraction and Characterization of Nanocrystalline Cellulose from Agricultural Residue-Oil Palm Petiole Fiber "

A Preliminary Study of Isolation and Characterization of Nanocrystalline Cellulose from Microcrystalline Cellulose by Acid Hydrolysis Process

This research focuses on the isolation of MCC (microcrystalline cellulose) into NCC (nanocrystalline cellulose) by acid hydrolysis process. The sulfuric acid hydrolysis (44 wt.% H2SO4) aims to fibrillation from MCC into NCC material. NCC has good properties such as high-surface-area, high-aspect-ratio, weight light, and reactive materials. The morphology of NCC was characterized by SEM (Scanning Electron Microscope) and TEM. The physical characterization was tested using FTIR, XRD, and TGA. The morphological result showed that the particle size of NCC was more homogeneous with a diameter size of 25±3 nm with 310±5 nm in length. The physical properties of NCC better slightly than MCC, indicated by the increasing crystallinity index value from 74.8 to 76.4%, and it has a high thermal resistance of 330°C.

Sugarcane Bagasse as the Source of Nanocrystalline Cellulose for Gelatin-Free Capsule Shell.

Gelatin-free capsules are possibly produced through an innovation which involves utilizing environmentally friendly materials derived from plants such as bagasse which are produced into nanocrystalline cellulose (NCC). This research was conducted to report the extraction and characterization of NCC from the abundant industrial plantation waste of sugarcane and its application as the base material for gelatin-free capsule shell material. The process involved using different concentrations of NCC at 1%, 2%, 4%, and 7% (in wt. %) with the addition of 1% hydroxypropyl methylcellulose (HPMC) (in wt. %) and 1% carbopol (in wt. %). Moreover, the NCC capsules obtained from sugarcane bagasse were tested for moisture content, tensile strength, elongation, solubility, and pH. The results showed that sugarcane bagasse contains 40–50% cellulose, 6.15%–9.5% moisture content which indicates they are potentially better in terms of storage, 7.25–7.85 pH, and 0.05–0.136 MPa gel strength, and the elongation value ranges from 7.19 to 87.51%. These values were discovered to have satisfied the standard requirements as indicated by the optimal concentration of 4% NCC +1% HPMC, which is in line with the Japanese Industrial Standard (JIS), thereby leading to the consideration of the material safe to be used as raw material in making capsule shells.

Preparation and Characterization of Nanocrystalline Cellulose from Cassava Stem Wastes by Electromagnetic Induction

Cassava stems were one of the largest agricultural by products in Indonesia, especially in Lampung Province. It is known that cassava stems have a fairly high lignocellulose content, especially cellulose which reaches 39.29%. The high cellulose content in cassava stems has great potential to be used as raw material for Nanocrystalline Cellulose (NCC). The preparation of nanocrystalline cellulose consists of four main stages, namely: pre-hydrolysis, delignification, bleaching, and acid hydrolysis. The pre-hydrolysis stage was carried out by boiling a solution of CH3COOH and cassava stem powder for 60 minutes at a temperature of 105oC. Cassava stem powder was then delignified using a 25% NaOH solution heated to a temperature of 105oC for 1 hour. The bleaching stage used a 3.5% NaOCl solution at a temperature of 50oC for 60 minutes and was carried out twice. The last step is acid hydrolysis using 2.5N HCl solution for 15 minutes at a temperature of 105oC, then the electromagnetic induction treatment is varied with temperature variations of 30oC, 50oC, and 70oC for 60 minutes. The prepared nanocrystalline cellulose were tested for lignocellulose, XRD and PSA. From the test results, the best variation of nanocrystal cellulose preparation was acid hydrolysis treatment with 70oC electromagnetic induction for 60 minutes, namely an increase in the percentage of cellulose 62.93%, crystallinity 90.68%, and an average particle size of 18.04µm with some particles measuring nanometers. From the results of the research, it was concluded that electromagnetic induction increased crystallinity and decreased the size of nanocrystalline cellulose.

Extraction, Purification and Characterization of Nanocrystalline Cellulose from Eichhornia crassipes (Mart.) Solms: A Common Aquatic Weed Water Hyacinth

ABSTRACT Eichhornia crassipes (Mart.) Solms, commonly called as water hyacinth, is a hydrophytic weed found in the tropical and subtropical areas. Eutrophication of the water bodies results due to its overgrowth. Many conservation steps have been taken to utilize this plant as biofertilizer, biogas and also for water purification. In the present investigation, nanocrystalline cellulose was extracted from Eichhorniacrassipes (Mart.) Solms through a series of processes viz., alkalization, bleaching, acid hydrolysis and sonication. After the chemical process, the obtained product at each stage was characterized to analyze the morphology, chemical characteristics and the thermal property. FTIR analysis reports the peaks at 1030 cm−1, 1055 cm−1 and 3000–2800 cm−1, which refer to C–O–C stretching vibration and C–H symmetric stretching validating the extracted cellulose and the efficacy of the process. The onset of degradation for raw water hyacinth and extracted cellulose occurs at 253.4°C and 226.9°C in thermogravimetric analysis. The particle size analysis analysis for sonicated sample shows that 93.02 nm proved that the sonicated sample is in nano size. In scanning electron microscopy, the morphology changes of each step processed water hyacinth and extracted cellulose have been studied.

Preparation and Characterization of Nanocrystalline Cellulose from Cladophora sp. Algae

Nanomaterial is one of the recent technological advancements that attracts the attention of many scientists. Many advanced properties could emerge from the utilization of materials on this scale. Nanocrystalline cellulose (NCC) is a cellulose-based nanomaterial currently gaining attention because of its remarkable properties. NCC was prepared by removing the amorphous part of cellulose through acid hydrolysis. Cladophora is a type of algae with a fiber-like body, that could grow rapidly and widely available in the Indonesian coastal area, which is considered a nuisance and has no known high economic value. Cladophora can be used as an alternative source of cellulose because it has a high amount of cellulose. Cellulose could be extracted from Cladophora using 17.5% of NaOH at 100°C, 1 M sulfuric acid hydrolysis at 100°C, and bleaching with 5% hydrogen peroxide at 100°C. Nanocrystalline cellulose could be extracted by sulfuric acid hydrolysis at 100°C, with a concentration of 2 M, 3 M, and 5 M. The duration of the process for each concentration, varied for 5, 10, and 15 hours. X-Ray Diffraction (XRD), Particle Size Analyzer (PSA), Transmission Electron Microscopy (TEM), and Fourier Transform Infrared (FTIR) spectroscopy were used to characterize the resulting hydrolyzed product. The result showed that the increase in acid concentration and processing duration increased the crystallinity while decreased particle length and cellulose diameter. The highest crystallinity and smallest particle size were 96.36% and 189.2 nm, obtained by acid hydrolysis in 5 M sulfuric acid for 15 hours.

Isolation and characterization of nanocrystalline cellulose from cocoa pod husk (CPH) biomass wastes

In this paper, cellulose nanocrystals (CNCs) were isolated from the cellulose extracted from cocoa pod husk waste, followed by characterization using XRD, FT-IR, TGA, SEM and TEM to determine its crystallinity, structural properties, thermal characteristics, morphology and dimensions respectively. The result revealed that the cocoa pod husk amorphous segments containing hemicelluloses and lignin were extensively removed with increasing chemical treatments leading to increased purity, crystallinity index and thermal stability of the extracted materials. The diameter, length and crystallinity index of the CNC isolated from the CPH are 10–60 nm, 41–155 nm and 67.60% respectively.

Isolation and Characterization of Nanocrystalline Cellulose from Ramie Fibers via Phosphoric Acid Hydrolysis

ABSTRACT Nanocrystalline cellulose (NCC) was successfully isolated using cellulose extracted from ramie fibers via chemical pretreatments followed by phosphoric acid hydrolysis. The effects of acid concentrations and hydrolysis times on the characteristics of the NCC were investigated in this work. The obtained NCCs were characterized using Fourier infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and thermogravimetric analysis (TGA). Fourier transform-infrared spectroscopy confirmed that hemicellulose and lignin were removed during the chemical pretreatments and hydrolysis. The results showed that the crystallinity index increased with increasing an acid concentration up to 16 M and then decreased drastically. The optimal acid concentration was found to be 16 M phosphoric acid at 150°C for 90 min during hydrolysis resulting in a rod-shaped NCC with a high crystallinity (89.28%), the average diameter (21.4 nm), and the average length (215.4 nm). The thermal stability was found to decrease with increasing acid concentration. The characteristic of NCCs was affected significantly by the acid concentration but not the hydrolysis time.

Extraction and Characterization of Nanocrystalline Cellulose (NCC) from Ramie Fiber by Hydrochloric Acid Hydrolysis

The present study investigates the structural, crystallinity index, crystallite size, morphological, and the dimension of Nanocrystalline Cellulose (NCC) extracted from ramie fiber by hydrochloric acid-hydrolysis. NCC was chosen because it has high strength and modulus compared to other natural materials. Ramie fiber was chosen because it has high cellulose content and abundant in Indonesia. The other reason is ramie has a high crystallinity index compared to other natural fibers. The extraction process was carried out into two steps: cellulose purification then followed by hydrochloric acid hydrolysis. The characterizations of extracted NCCs were conducted through Fourier-Transform Infrared spectroscopy (FTIR), X-ray diffraction (XRD), Transmission Electron Microscopy (TEM), and Particle Size Analyzer (PSA). The focus of this research was to study the effect of acid concentration on the characteristics of extracted NCCs. The results show that NCCs could be extracted through hydrolysis using hydrochloric acid. Morphological analysis by TEM shows the formation of rod-like shaped NCCs. Hydrolysis by hydrochloric acid does not affect the chemical compound of cellulose. It was found that during hydrolysis, the higher acid concentration being employed had resulted in smaller dimension of NCCs and decreasing crystallinity index.

Isolation and characterization of nanocrystalline cellulose from flaxseed Hull: A future onco-drug delivery agent

Cellulose is a polysaccharide composed of D-glucopyranose linked by 1,4 β-glycoside bond with three hydroxyl groups. These hydroxyl groups in cellulose have an important role in the compactness of crystalline structure and in determining the physical properties of cellulose. Cellulose in nanometers size range from 10 nm to 350 nm is known as nano cellulose, which has a variety of applications due to the unique properties such as low density, biodegradable, and good mechanical properties. In the present study, we present the isolation of the nano cellulose from flaxseed hull for the first time. The isolated nano cellulose was characterized by techniques such as UV–Vis, FT-IR, BET, XRD SEM, and TEM. The nano cellulose obtained was found to be crystalline in nature with a crystallinity index of 46% and the surface area of 5 cm2/g with excellent thermal stability.

A Preliminary Study of Extraction and Characterization of Nanocrystalline Cellulose (NCC) from Ramie Fiber

Nanocrystalline cellulose (NCC) is today’s one of rapidly growing nanocomposite reinforcing materials. This is thanks to some advantages that come with it over anorganic nanocomposite reinforcing materials, for example, nanoclay, nanosilica, nanoalumina, carbon nanotubes, among others. Some of the advantages of NCC are environment-friendliness due to being organic-compound-based, high mechanical property, and easy manufacturing. NCC can be extracted from natural cellulose sources, such as natural fiber, wood, and animals. One of the natural fibers rich in cellulose content, in this case around 80%, is fiber of ramie which is ubiquitous in Indonesia. It is well known that the acid hydrolysis method is an easy route to NCC fabrication. A number of chemical treatments like de-waxing, bleaching, and alkaline treatment are typically performed prior to acid hydrolysis process. The effect of such chemical treatments as precursors of alkaline hydrolysis on ramie fiber characteristics was investigated in this research. Firstly, ramie fiber was cut 1 cm in size, then grinded and sieved. The fiber was then subjected to de-waxing process by adding it into a toluene-ethanol solution (1:2). Then, it was submitted to bleaching with 0.7% sodium chlorite (NaClO2) solution at 75 °C for 1 hour. Lastly, the fiber was subjected to an alkaline treatment in 2% NaOH solution for 2 hours. Characterization with FT-IR, XRD, and TGA of the fiber which had underwent a number of surface treatments was conducted. Results show that the chemical treatments had successfully removed amorphous components like lignin and hemicellulose from the ramie fiber. Chemical treatments were proven able to increase the crystallinity index and thermal stability of ramie fiber.

Production and characterization of nanocrystalline cellulose from palm empty fruit bunch fiber

The main objective of this research is to utilize fiber waste from palm empty fruit bunch provided very abundant in Aceh. For that needs to be studied and researched other benefits are more valuable. Among them is a source of raw material to produce nanocrystalline cellulose (NCC), a material in nanometer dimensions which can be used as fillers or reinforcing a matrix in order to provide bio nanocomposites. NCC isolated from palm empty fruit bunch through the hydrolysis of sulfuric acid, using a solvent mixture of DMAC/LiCl, were passed on activated dialysis membrane after centrifuged to release it from solvent. NCC will be characterized include aspect ratio, morphology, thermal, structural test, and several other physical parameters. The end product is characterized by means of thermogravimetric analysis (TGA), Infrared Spectroscopy (FTIR), X-Ray Diffraction (XRD), Differential Scanning Calorimetry (DSC) and Scanning Electron Microscopy (SEM). From XRD and FTIR analysis showed that the nanocrystalline cellulose formed was Nano cellulose I with an average size of 72 nm. DSC and TGA thermal test showed that the glass transition, melting point, and decomposition temperature of NCC were 109.59 ° C, 191.28 ° C, 210 °C, respectively.

Preparation and characterization of nanocrystalline cellulose using ultrasonic assisted autohydrolysis

Cellulose is a biopolymer whose availability is abundant in nature. In its development, cellulose is widely used in the form of nanocrystalline cellulose because it has better properties than the original material. The method commonly used to produce nanocrystalline cellulose is acid hydrolysis. In this study, nanocrystalline cellulose was produced through a method of modification of acid hydrolysis with hydrothermal treatment by autoclave, then ultrasonic treatment was conducted. The results of nanocrystalline cellulose were analyzed by particle size analyzer (PSA), scanning electron microscope-energy Dispersive X-ray spectroscopy (SEMEDS), Fourier-transformed infrared spectra (FT-IR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA).

Isolation and characterization of nanocrystalline cellulose from roselle-derived microcrystalline cellulose

Roselle fiber is a renewable and sustainable agricultural waste enriched with cellulose polysaccharides. The isolation of Nanocrystalline cellulose (NCC) from roselle-derived microcrystalline cellulose (MCC) is an alternative approach to recover the agricultural roselle plant residue. In the present study, acid hydrolysis with different reaction time was carried out to degrade the roselle-derived MCC to form NCC. The characterizations of isolated NCC were conducted through Fourier Transform Infrared Ray (FTIR), Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FESEM), Atomic Force Microscopy (AFM), Dynamic Light Scattering (DLS), Energy Dispersive Spectroscopy (EDS), X-ray Diffraction (XRD), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC). As evaluated from the performed morphological investigations, the needle-like shape NCC nanostructures were observed under TEM and AFM microscopy studies, while irregular rod-like shape of NCC was observed under FESEM analysis. With 60min hydrolysis time, XRD analysis demonstrated the highest NCC crystallinity degree with 79.5%. In thermal analysis by TGA and DSC, the shorter hydrolysis time tended to produce NCC with higher thermal stability. Thus, the isolated NCC from roselle-derived MCC has high potential to be used in application of pharmaceutical and biomedical fields for nanocomposite fabrication.

Isolation and characterization of nanocrystalline cellulose from sugar palm fibres (Arenga Pinnata)

Cellulose was extracted from sugar palm fibres (Arenga pinnata) by conducting delignification and mercerization treatments. Subsequently, sugar palm nanocrystalline celluloses (SPNCCs) were isolated from the extracted cellulose with 60wt% concentrated sulphuric acid. The chemical composition of sugar palm fibres were determined at different stages of treatment. Structural analysis was carried out by Brunauer-Emmett-Teller (BET), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). Morphological analysis of extracted cellulose and isolated nanocrystalline cellulose (NCCs) was investigated by using field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), and transmission electron microscopy (TEM). The thermal stability of sugar palm fibres at different stages of treatment was investigated by thermogravimetric analysis (TGA). The results showed that lignin and hemicellulose were removed from the extracted cellulose through the delignification and mercerization process, respectively. The isolated SPNCCs were found to have length and diameters of 130±30nm and 9±1.96nm, respectively.

XRD and FTIR Studies of Nanocrystalline Cellulose from Water Hyacinth (<i>Eichornia crassipes</i>) Fiber

The isolation and characterization of nanocrystalline cellulose (NCC) from water hyacinth (WH) fibers were carried out. There are two treatments to obtain NCC from WH fibers by chemical and mechanical treatments. The chemical treatment involved alkalization with NaOH 25% in a highly-pressured tube, acid hydrolysis with 5M HCl, and bleaching with (NaClO2:CH3COOH) in ratio 5:2. The mechanical treatment was performed by using ultrasonic homogenizing at 12000 Rpm for 2 h. The morphological surface was observed by Transmission Electron Microscopy (TEM). TEM reported that the size of NCC was 10–40 nm. Crystallinity index and functional group analysis of the NCC WH fibers were also examined using X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) techniques. XRD reported that the crystallinity index increased significantly after chemical and mechanical treatment due to the presents of crystalline area in the WH fibers. The crystallinity index of raw fiber, digester, bleaching, and ultrasonic homogenizing were 7%, 68%, 69%, and 73% respectively. The content cellulose of final product was 68% as measured by the chemical composition test. Meanwhile, FTIR reported that WH fibers after being given chemical treatment lead the functional group change due to removal hemicellulose and lignin. The result of XRD and FTIR were indicated that the sample of NCC WH fibers presents the structure of cellulose crystal type I.

Extraction, isolation and characterization of nanocrystalline cellulose from industrial kelp (Laminaria japonica) waste

Kelp (Laminaria japonica) is an economically important type of algae cultured in East Asia. Kelp waste is a by-product from the extraction of commercial alginate from kelp. This work reports the isolation of nanocrystalline cellulose (NCC) from the cellulose extracted from the kelp waste. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) show that the crystallinity index of the isolated kelp NCC was 69.4%, which was slightly higher than that of kelp cellulose as well as maintained the cellulose I crystalline form and typical cellulose chemical structure. In thermogravimetric analysis (TGA), kelp NCC showed decreased thermostability and a higher residual mass. Transmission electronic microscopy (TEM) confirmed the ordinary rod-like shape of the produced NCC with various dimensions. The kelp NCC aqueous dispersions displayed the expected characteristic optical and gel effects. Studies on the variables and the orthogonal experiment of NCC preparation contributed a maximum yield of 52.3%. The exploration on the preparation of kelp NCC in this study lays foundations for future applications.

Extraction and Characterization of Nanocrystalline Cellulose from Cassava Bagasse

The objective of this study was to produce highly crystalline nanocellulose from cassava bagasse, which is a by-product from the cassava starch industry that contains 15 to 50 wt% cellulose fibers. For this purpose, the lignocellulosic fiber that was isolated from cassava bagasse was bleached with sodium chlorite and hydrolyzed with sulfuric acid. The obtained suspension was spray-dried and the resulting microparticles were characterized by X-ray diffractometry to determine the crystallinity index. Microcrystalline cellulose was subjected to the same processing conditions and considered as a reference. The crystallinity index of the nanocellulose extracted from the cassava bagasse was higher than the crystallinity index of the nanocellulose extracted from the microcrystalline cellulose; 84.1 and 78.7%, respectively. No other studies have reported cellulose nanocrystals from cassava bagasse with a high crystallinity index. The nanocrystalline cellulose produced in this study is very promising for use in innovative industrial applications.