Characteristics Of New Line Research Articles

Influence of weather conditions on the manifestation of quantitative and qualitative characteristics of new lines of oil flax

Influence of weather conditions on the manifestation of quantitative and qualitative characteristics of new lines of oil flax

Comparative characteristics of new lines of mesenchymal stem cells derived from human embryonic stem cells, bone marrow, and foreskin

New human nonimmortalized fibroblast-like cell lines were derived from various sources: from embryonic stem cells (ESCs) (the SC5-MSC and SC3a-MSC lines), from bone marrow of a 5- to 6-week-old fetus (the FetMSC line), and from foreskin of a 3-year-old child (the FRSN line). All the lines are successfully used as feeders during cultivation of human ESCs. The mean doubling time of the cell populations fluctuates depending on the line from 25.5 h in the SC5-MSC line to 38.8 h in the SC3a-MSC line. The growth curves indicate active cell proliferation of all lines. Numerical and structural karyotypical analysis has shown these lines to have a normal karyotype: 46, XX (SC5-MSC and SC3a-MSC) and 46, XY (FetMSC and FRSN). To determine the status of these lines, comparative analysis of surface markers was performed with the aid of flow cytofluorimetry, and expression of the c antigens characteristic of human mesenchymal stem cells (MSCs) was revealed: CD44, CD73, CD90, CD105, and HLA-ABC and the absence of expression of CD34 and HLA-DR. Interlinear differences in the expression level of the marker CD117 (c-kit) were revealed. Immunofluorescent and cytofluorimetric analysis of expression of surface markers and transcription factor Oct-4 that are characteristic of human ESCs has shown that, in all four lines, expression of TRA-1-60 and Oct-4 is absent, whereas in expression of SSEA-4 there are observed the interlinear differences not depending on the origin of cells. At present it is not yet clear whether the revealed interlinear differences affect essentially the functional status of mesenchymal stem cells. Immunofluorescent analysis in cells of all lines showed expression of markers of early differentiation into derivatives of three germ layers characterizing ESCs, which might possibly provide wide MSC possibilities during reparation of different tissue damages, depending on the corresponding microenvironment. The capability of cells of all lines for directed differentiation into the adipogenic and osteogenic directions was revealed.

Rice varieties in relation to rice bread quality

It is difficult to predict rice bread quality only from the amylose content (AC) or dough characteristics of new lines produced by rice breeding programmes. This study investigated the AC relative to bread baking quality of rice varieties developed in Korea, and identified specific characteristics that contribute to rice bread quality. Manmibyeo, Jinsumi, Seolgaeng and Hanareumbyeo were classified as low AC, YR24088 Acp9, Suweon517, Chenmaai and Goamibyeo as intermediate AC and Milyang261 as high AC. Suweon517, Milyang261 and Manmibyeo had a high water absorption index (WAI), while Goamibyeo, YR24088 Acp9, Jinsumi, Seolgaeng, Hanareumbyeo and Chenmaai had a low WAI. The gelatinisation enthalpy of flour varied from 9.2 J g(-1) in Milyang261 to 14.8 J g(-1) in YR24088 Acp9. After 7 days of storage the rate of flour retrogradation and crumb firmness were weakly correlated, with the exception of Jinsumi. Bread volumes of Jinsumi, Chenmaai, YR24088 Acp9 and Goamibyeo were comparable to that of wheat flour, but the rest were unsuited to bread making because of their low volume and hard crumb texture. Based on volume, texture and crumb firmness ratio, Chenmaai and Goamibyeo were the most appropriate varieties for making bread. An intermediate AC and low WAI were the primary indicators of rice bread flour quality.

Evaluation of the Agronomical and Biochemical Characteristics of New Lines of Bread Wheat in Turkey

Sixteen new lines of bread wheat obtained from the CIMMYT/ICARDA and a standard cultivar were evaluated during three successive years (2003, 2004 and 2005). The experiments were conducted at the experimental farm of the Faculty of Agriculture of the Uluda? University in Bursa. The lines and the standard cultivar showed significant differences in plant height, number of seeds/spike, seed weight/spike, seed yield and a 1000-kernel weight. The lines numbered 4, 6, 7, 9, 11, 14 and 15 produced significantly higher seed yields than the standard cultivar. Therefore, they formed the highest group (a) of seed yield. Similarly, these lines had a higher number of seeds/spike value than the standard cultivar. However, lines 6 and 11 produced higher seed yields than lines 4, 7, 9, 14 and 15. In particular, the gluten composition and the zeleny sedimentation test values of whole flours revealed that lines 6 and 11 could be suitable for high-quality pasta production with good sensory characteristics. To summarize, there were significant differences between the lines with respect to agronomic and biochemical characteristics. In particular, lines 6 and 11 had better performance than both the other lines and the standard cultivar because of their seed yield and quality.

Evaluation of chemical and technological characteristics of new lines of Triticum turgidum ssp dicoccum.

Triticum durum ssp dicoccum also called farro in Italy is gaining more and more interest from farmer due to its commercial potentiality. In this paper, thirty lines F6 of farro obtained by crossing the cvs. Triticum turgidum ssp durum Ofanto and Simeto and T. turgidum ssp dicoccum Molise population were studied. They were evaluated with special reference to chemical composition and technological characteristics in order to select genotypes suitable for the production of food commodities. The storage protein composition (High Molecular Weight and Low Molecular Weight Glutenin) was also considered by different electrophoretic techniques.