Changes Of Human Dentin Research Articles

Optical changes of human dentin after non-vital bleaching and effect of Er,Cr:YSGG laser on micro-shear bond strength of a self-etch and an etch-and-rinse adhesive system.

The aim was to evaluate the optical changes of bleached human dentin and the efficacy of Er,Cr:YSGG laser on micro-shear bond strength (μSBS) of two adhesive systems for immediate and delayed applications. Seventy-two human dentin specimens (1mm) were obtained. Colour measurements of 30 specimens (control and bleaching groups) were made at baseline, after bleaching and a 7-day delay. Differences at colour (ΔE00), translucency (ΔTP00) and whiteness (ΔWID) were calculated by recorded data. Forty-two specimens were used to compare μSBS of a self-etch (Clearfil SE Bond [SE]) and an etch-and-rinse (Prime&Bond NT [PBNT]) adhesive to bleached dentin. Additional to a negative group, six groups were contemplated for each adhesive, considering the time of composite application (immediate, delayed) and whether additional surface treatment by laser (1W, 2W) or not. Colour and μSBS data were statistically analysed. For optical analysis, only ΔE00 and ΔWID calculated for the difference between baseline and bleaching were found significantly different for control and bleaching groups. Whiteness of bleached specimens was remarkably decreased after a 7-day delay. Despite immediate applications of SE showing lower μSBS than negative control, delayed applications showed no significant differences except that of the 2W laser etching. For PBNT, laser etching increased μSBS for both of the immediate and delayed applications, and no significant difference was observed from negative control. Related with the preferred adhesive system, Er,Cr:YSGG laser etching can be an alternative for optimal bond strength of immediate restorations, but the clinicians should always keep in mind that perceived colour and whiteness will change by the passing time which can affect the shade match.

Measurement of biomechanical behavior of dentin hard tissue in response to unbound water loss using stereo-digital image correlation

Understanding the biomechanical behavior of dentin hard tissue with fluid-filled dentin tubules and hydrated matrices is essential for studying this functionally graded biological composite. The stereo-digital image correlation technique with an adaptive high-magnification field of view (FOV) for fully hydrated biological tissue measurement was investigated. The adaptive magnification is controlled by the length of extension tubes. To determine both the unbound water loss induced and load-induced three-dimensional (3D) deformation of dentin hard tissue from a fully hydrated state to a non-hydrated condition, samples of dentin blocks and half teeth in sagittal sections were studied for a period of 2 h in situ over varied speckle patterns. The effects of speckles on water evaporation, camera pre-heating, and measurement accuracy in the wet, curved and long-term measurement were analyzed. The elastic modulus and Poisson’s ratio of both dentin and pulp in response to unbound water evaporation were measured. With the unbound water loss, the mean values of the elastic modulus generally increased from ∼8 GPa to ∼10 GPa in pulp region and from ∼10 GPa to ∼12 GPa in dentin region. The mean values of the Poisson’s ratio increased both in pulp and in dentin. Poisson’s ratio in the dentin regions (∼0.3) were generally smaller than those in the pulp regions (even can reach 0.6), irrespective of the partial dehydration time. Further analysis of the full-field deformation results provided insight into the unbound water-induced regional deformations and mechanical changes in human dentin. It’s found that the unbound water loss induced deformations were more prominent when compared to load induced deformations.

Whole-field macro- and micro-deformation characteristic of unbound water-loss in dentin hard tissue.

High-resolution deformation measurements in a functionally graded hard tissue such as human dentin are essential to understand the unbound water-loss mediated changes and their role in its mechanical integrity. Yet a whole-field, 3-dimensional (3D) measurement and characterization of fully hydrated dentin in both macro- and micro-scales remain to be a challenge. This study was conducted in 2 stages. In stage-1, a stereo-digital image correlation approach was utilized to determine the water-loss and load-induced 3D deformations of teeth in a sagittal section over consecutively acquired frames, from a fully hydrated state to nonhydrated conditions for a period up to 2 hours. The macroscale analysis revealed concentrated residual deformations at the dentin-enamel-junction and the apical regions of root in the direction perpendicular to the dentinal tubules. Significant difference in the localized deformation characteristics was observed between the inner and outer aspects of the root dentin. During quasi-static loadings, further increase in the residual deformation was observed in the dentin. In stage-2, dentin microstructural variations induced by dynamic water-loss were assessed with environmental scanning electron microscopy and atomic force microscopy (AFM), showing that the dynamic water-loss induced distention of dentinal tubules with concave tubular edges, and concurrent contraction of intertubular dentin with convex profile. The findings from the current macro- and micro-scale analysis provided insight on the free-water-loss induced regional deformations and ultrastructural changes in human dentin.

Structural and Morphological Changes in Human Dentin after Ablative and Subablative Er:YAG Laser Irradiation.

This study investigated the influence of Erbium-Doped Yttrium Aluminum Garnet (Er:YAG) laser on microhardness, chemical composition and subsurface morphology of dentin cavity walls. Forty sound human premolars were selected and randomly assigned into four groups. Class V cavities were prepared either with an Er:YAG laser (groups 1 and 2; 15 Hz, 250 mJ for enamel, 10 Hz, 200 mJ for dentin) or with a high speed handpiece (groups 3 and 4). The specimens in groups 1 and 3 served as the control, whereas those in groups 2 and 4 were exposed to subablative laser irradiation following cavity preparation (10 Hz, 50 mJ). After bisecting the specimens, one half was subjected to microhardness assessment and the other half was evaluated by SEM-EDS analysis. Microhardness was significantly greater in the specimens prepared by both ablative and subablative laser irradiation (group 2) than that of the bur-prepared cavities (groups 3 and 4) (P < 0.05). The quantity of calcium ion was significantly greater in cavities prepared by the Er:YAG laser (groups 1 and 2) compared to that of the bur cavities (groups 3 and 4) (P < 0.05). Subablative irradiation improved microhardness and weight percentage of calcium ion in both laser and bur cavities, but the difference was not significant compared to that of the relevant control group (P > 0.05). Cavity preparation with an Er:YAG laser could be considered as an alternative to the conventional method of drilling, as it enhances the mechanical and compositional properties of lased dentin, especially when combined by subablative irradiation.

Chemical and morphological changes in human dentin after Er:YAGlaser irradiation: EDS and SEM analysis.

Sixty samples of human dentin were divided into six groups (n = 10) and were irradiated with Er:YAG laser at 100 mJ-19.9 J/cm(2), 150 mJ-29.8 J/cm(2), 100 mJ-35.3 J/cm(2), 150 mJ-53.0 J/cm(2), 200 mJ-70.7 J/cm(2), and 250 mJ-88.5 J/cm(2), respectively, at 7 Hz under a water spray. The atomic percentages of carbon, oxygen, magnesium, calcium, and phosphorus and the Ca-to-P molar ratio on the dentin were determined by energy dispersive X-ray spectroscopy. The morphological changes were observed using scanning electron microscopy. A paired t-test was used in statistical analysis before and after irradiation, and a one-way ANOVA was performed (P ≤ 0.05). The atomic percent of C tended to decrease in all of the groups after irradiation with statistically significant differences, O and Mg increased with significant differences in all of the groups, and the Ca-to-P molar ratio increased in groups IV, V, and VI, with statistically significant differences between groups II and VI. All the irradiated samples showed morphological changes. Major changes in the chemical composition of dentin were observed in trace elements. A significant increase in the Ca-to-P ratio was observed in the higher energy density groups. Morphological changes included loss of smear layer with exposed dentinal tubules. The changes produced by the different energy densities employed could have clinical implications, additional studies are required to clarify them.

Morphological Changes of Human Dentin after Erbium-Doped Yttrium Aluminum Garnet (Er:YAG) and Carbon Dioxide (CO2) Laser Irradiation and Acid-etch Technique: An Scanning Electron Microscopic (SEM) Evaluation.

The aim of this study was to investigate the morphological changes of human dentin after Erbium-Doped Yttrium Aluminum Garnet (Er:YAG), Carbon Dioxide(CO2) laser-irradiation and acid-etching by means of scanning electron microscopic (SEM) Methods: 9 extracted human third molars were used in this study. The teeth were divided in three groups: first group, CO2 laser with power of 1.5 w and frequency of 80 Hz; second group, Er:YAG laser with output power of 1.5 W frequency of 10 Hz, very short pulse with water and air spray was applied; and third group, samples were prepared by acid-etching 37% for 15 sec and rinsed with air-water spray for 20 sec. Then, the samples were prepared for SEM examination. Melting and cracks can be observed in CO2 laser but in Er:YAG laser cleanedablated surfaces and exposed dentinal tubules, without smear layer was seen. It can be concluded that Er:YAG laser can be an alternative technique for surface treatment and can be considered as safe as the conventional methods. But CO2 laser has some thermal side effects which make this device unsuitable for this purpose.

Changes of Human Dentin by Brushing with Commercial Dentifrices for Sensitive Teeth in vitro

Changes of Human Dentin by Brushing with Commercial Dentifrices for Sensitive Teeth in vitro

Effects of heating on the mechanical and chemical properties of human dentin

ObjectivesWe had previously discovered that the flexural and tensile strengths of human dentin were 2–2.4 times greater after being heated to 140°C, and deduced that the generation of higher-density structures and therefore dehydration probably promoted the increased strength. Our test hypotheses were that intertubular dentin, which constitutes a major part of organic components, was selectively affected by heating, and such changes could happen without critical damages to the basic structure of dentin type I collagen. MethodsMicro-mechanical changes of human dentin by heating at 140°C were investigated by nano-indentation. Chemical changes in dentin collagen after heating were also investigated by X-ray diffraction study, a microscopic Fourier transform infrared (micro-FTIR) and a laser Raman spectroscopic analyses, and a cross-linking analysis by high-performance liquid chromatography. ResultsThe results of nano-indentation showed that the micro-hardness of intertubular dentin increased after heating at 140°C to 1.8 times more than unheated dentin; on the other hand, peritubular dentin was unchanged. Results of X-ray diffraction showed that the lateral packing of collagen molecules shrank from 13.6±0.3 to 10.6±0.1Å after heating, but the shrinkage reversed to the original after rehydration for seven days. After heating, no substantial chemical changes in the collagen molecules were detected in tests by micro-FTIR or Raman analyses, or by cross-linking analysis. SignificanceThese results suggest that intertubular dentin, which contains most of the type I collagen, was selectively affected by heating at 140°C without critical damage to its collagen.

Compositional and morphological changes of human dentin after Er:YAG laser irradiation

To date, no evaluation has been reported about the phase and compositional changes of human dentin after Er:YAG laser irradiation. The aim of this study was to investigate the phase, compositional, and morphological changes of Er:YAG laser-irradiated dentin with or without water spray by means of X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). The irradiation energies were from 300 to 700 mJ/pulse-10 pps-10 s. After exposure to Er:YAG laser, dentin showed no phase changes on the X-ray diffractometer. In Fourier transform infrared analysis, two absorption bands at 2200 and 2015 cm −1 could be traced on dentin treated by Er:YAG laser with the irradiation energies beyond 300 mJ/pulse-10 pps-10 s and without water spray. The disappearance of OH − band at 1630 cm −1 could also be found. Scanning electron micrographs revealed that the laser energy of 500 mJ/pulse-10 pps-10 s was sufficient to prompt melting and recrystallization of dentin crystals. Therefore, water spray is important for reducing the thermal effect of Er:YAG laser.

Ultrastructural changes of human dentin after irradiation by Nd:YAG laser.

The use of Nd:YAG laser has been proposed for endodontic treatment. However, its ability to reduce dentin permeability, which is important for the success of root canal treatment, remains controversial. Nd:YAG laser irradiation was performed in pulsed mode on human dentin. The parameters were: pulse energy (100 mJ), rate (10 pps), and total irradiation time (4 seconds). The crystalline phases, electron diffraction patterns, morphology, and microstructure of specimens after laser irradiation were observed by dark-field emission transmission electron microscope (TEM). Three ultrastructural zones could be delineated in the dentin: (1) an outer zone with an ordered columnar structure composed of hydroxyapatite and beta-tricalcium phosphate, (2) an intermediate zone composed of an amorphous substance (about 40-70 nm in diameter), and (3) an inner zone of well-crystallized hydroxyapatite grains. These three zones were free of pores or voids. Our study demonstrated that laser-irradiation might be used to reduce dentin permeability.

Phase, Compositional, and Morphological Changes of Human Dentin after Nd:YAG Laser Treatment

Although techniques for repairing root fracture have been proposed, the prognosis is generally poor. If the fusion of a root fracture by laser is possible, it will offer an alternative to extraction. Our group has attempted to use lasers to fuse a low melting-point bioactive glass to fractured dentin. This report is focused on the phase, compositional, and morphological changes observed by means of X-ray diffractometer, Fourier transforming infrared spectroscopy, and scanning electron microscopy-energy dispersive X-ray spectroscopy in human dentin after exposure to Nd:YAG laser. The irradiation energies were from 150 mJ/ pulse-10 pps-4 s to 150 mJ/pulse-30 pps-4 s. After exposure to Nd:YAG laser, dentin showed four peaks on the X-ray diffractometer that corresponding to a-tricalcium phosphate (TCP) and beta-TCP at 20 = 30.78 degrees/34.21 degrees and 32.47 degrees/33.05 degrees, respectively. The peaks of a-TCP and beta-TCP gradually increased in intensity with the elevation of irradiation energy. In Fourier transforming infrared analysis, two absorption bands at 2200 cm(-1) and 2015 cm(-1) could be traced on dentin treated by Nd:YAG laser with the irradiation energies beyond 150 mJ/pulse-10 pps-4 s. The energy dispersive X-ray results showed that the calcium/phosphorus ratios of the irradiated area proportionally increased with the elevation of irradiation energy. The laser energies of 150 mJ/ pulse-30 pps-4 s and 150 mJ/pulse-20 pps-4 s could result in the a-TCP formation and collagen breakdown. However, the formation of glass-like melted substances without a-TCP at the irradiated site was induced by the energy output of 150 mJ/ pulse-10 pps-4 s. Scanning electron micrographs also revealed that the laser energy of 150 mJ/ pulse-10 pps-4 s was sufficient to prompt melting and recrystallization of dentin crystals without cracking. Therefore, we suggest that the irradiation energy of Nd:YAG laser used to fuse a low melting-point bioactive glass to dentin is 150 mJ/ pulse-10 pps-4 s.

Changes of Human Dentin by Brushing with Several Kinds of Commercial Dentifrices in vitro

Changes of Human Dentin by Brushing with Several Kinds of Commercial Dentifrices in vitro

Mineral Distribution and Dimensional Changes in Human Dentin during Demineralization

Many bonding agents require the dentin surface to be acid-etched prior to being bonded. Understanding the stability and morphology of the etched dentin surface is important for improving bond strength and reliability in these systems. In this study, the atomic force microscope was used to quantify dimensional changes that occur to fully hydrated dentin during demineralization with a pH 4.0 lactic acid gel. A high-resolution microtomography instrument, the x-ray tomographic microscope, was also used to quantify the mineral density distribution in the dentin as a function of etching time. The intertubular dentin surface shrank by less than 0.5 microns during etching, while the peritubular dentin receded at an initially rapid linear rate. The dentin surface retained its initial morphology, although it was more porous with the removal of the peritubular dentin. Beneath the etched surface, there were three major zones characterized by mineral density differences. The first zone was a fully demineralized collagen layer, subjacent to which was a partially demineralized zone of roughly constant mineral density. Immediately following the partially mineralized layer was normal dentin. The presence of the partially mineralized layer could be explained in terms of different transport rates in the peritubular and intertubular dentin.

Nd: YAGレーザー照射を象牙質表面に与えた時の象牙質及び歯髄の変化

Nd: YAGレーザー照射を象牙質表面に与えた時の象牙質及び歯髄の変化