Changes In Water Temperature Research Articles

Cold Tolerance and Physiological Response of Natural Overwintering Pomacea canaliculata in South China.

Pomacea canaliculata (Lamarck 1822), a freshwater gastropod indigenous to lower Del Plata Basin of Argentina, has become the most destructive and invasive rice pests in south China since its introduction in the 1980s. In Guangdong, the main production areas for double rice, most of P. canaliculata overwinter in paddy field ditches after late-rice harvesting in mid-November and diapause to temporarily to avoid the damaging effects of extreme low temperatures. This pest aroused from diapause and migrated to the paddy field after early-rice reviving in next late March. Overwintering and cold tolerance of natural P. canaliculata have a non-negligible impact on population dynamics and distribution in the following year. We tested the supercooling capability, levels of cryoprotectant synthesis, activity of antioxidant defense system (antioxidant enzymes and reduced glutathione), and degree of oxidative damage (concentration of malondialdehyde as an index of lipid peroxidation) monthly, using natural P. canaliculata samples with a size-gender structure (i.e., juveniles, female, and male adults) from experimental ponds during the period of mid-November to the following April. P. canaliculata survived the winter with a monthly death rate of 7%-16.5% in coldest January. The supercooling point (SCP) of overwintering P. canaliculata decreased initially before increasing subsequently with monthly changes in water temperature. P. canaliculata accumulated a high glycogen content before December, which depleted towards the end of January, while lipid content reached peak in January and depleted since February. Activity of antioxidant defense system of P. canaliculata exhibited significant monthly differences and showed relatively higher size heterogeneity than monthly variations. The results contribute to the knowledge of adaptability in overwintering P. canaliculata and help to understand the mechanism of the invasive success of this species.

Environmental Drivers of the Divergence of Harveyi Clade Pathogens with Distinctive Virulence Gene Profiles

Fish and shellfish pathogens of the Harveyi clade of the Vibrio genus cause significant losses to aquaculture yields and profits, with some of them also causing infections in humans. The present study aimed to evaluate the presence of Harveyi clade fish and shellfish pathogens and their possible diversification in response to environmental drivers in southeastern USA waters. The presence and abundance of potential pathogens were evaluated via the detection and quantitation of six Harveyi-clade-specific virulence genes (toxR, luxR, srp, vhha, vhh, and vhp; VGs) in environmental DNA with clade-specific primers. The environmental DNA was obtained from water and sediments collected from three Georgia (USA) cultured clam and wild oyster grounds. In sediments, the VG concentrations were, on average, three orders of magnitude higher than those in water. The most and least frequently detected VGs were vhp and toxR, respectively. In water, the VGs split into two groups based on their seasonal trends. The first group, composed of luxR, vhp, vhha, and vhh, peaked in August and remained at lower concentrations throughout the duration of the study. The second group, composed of toxR and srp, peaked in June and disappeared between July and December. The first group revealed a high adaptation of their carriers to an increase in temperature, tolerance to a wide range of pH, and a positive correlation with salinity up to 25 ppt. The second group of VGs demonstrated a lower adaptation of their carriers to temperature and negative correlations with pH, salinity, potential water density, conductivity, and dissolved solids but a positive correlation with turbidity. No such trends were observed in sediments. These data reveal the role of VGs in the adaptability of the Harveyi clade pathogens to environmental parameters, causing their diversification and possibly their stratification into different ecological niches due to changes in water temperature, acidity, salinity, and turbidity. This diversification and stratification may lead to further speciation and the emergence of new pathogens of this clade. Our data urge further monitoring of the presence and diversification of Harveyi clade pathogens in a global warming scenario.

Variability in Water Temperature Vertical Distribution and Advective Influences: Observations from Early Summer 2021 in the Central Yellow Sea

To analyze variations in the vertical distribution of water temperatures and the impact of advection in the central Yellow Sea, multi-layer water temperature and current observations were conducted from 31 May to 8 June 2021. Water temperatures exhibited a typical three-layer summer structure, with a uniform deep-layer temperature averaging 8.23 ± 0.05 °C. The current field was dominated by northeast–southwest tidal currents, but residual current characteristics indicated that non-tidal components significantly influenced circulation. Water temperature changes lagged tidal changes by about 3 h, with strong correlations (R > 0.7), especially in deep layers. Residual currents showed significant correlations with water temperature variations, which were attributed to advective displacement or baroclinic currents. Empirical orthogonal function (EOF) and complex EOF analyses revealed that thermocline variations (T1, explaining approximately 75% of total variance) were driven by strong northward (C1, approximately 34%) and cyclonic (C2, approximately 32%) advection. In deep layers, slight temperature changes were caused by southward Yellow Sea Cold Water Mass (C1) and northward Yellow Sea Warm Current Water (C2) propagation. This study confirms that vertical water temperature variations result from a complex interaction between various advection patterns, with southward tide-induced residual currents (C3, approximately 12%) playing a key dynamic role.

AN EFFICIENT HIGH POWER WATER DUMMY LOAD AT 2.45 GHz

A water dummy load developed as an element of equipment in the composition of a microwave atmospheric pressure plasmatron operating at a frequency of (2450±50) MHz is described. The load is created on the base of a rectangular waveguide with a cross-section of 90x45 mm. The simulation results carried out by CST Microwave Studio shows that the return losses reach -42 dB at 2.45 GHz. In the frequency band from 2 to 2.9 GHz, the load provides a voltage standing wave ratio (VSWR) of at least 1.2. The effect of changes in water temperature and salinity on the device characteristics is studied. The load is manufactured and successfully used as part of experimental waveguide paths.

Seasonal Variability of Human Enteric Viruses Discovered in Food Production Mussels (Mytilus galloprovincialis) Farmed in the Central Adriatic Sea (Italy).

Among the different naked and quasi-enveloped viruses, the hepatitis A virus (HAV), hepatitis E virus (HEV), and norovirus genogroups I and II (NoV GI and NoV GII) are considered the main microbiological noxae involved in foodborne outbreaks. Mussels can harbor pathogens in their tissues. In addition to epidemiological attention, marine water temperature changes are considered a crucial variable influencing viral loads. This study aimed to biomolecularly screen 1775 farmed mussels (Mytilus galloprovincialis) for viral ribonucleic acid (RNA) sequence detection (belonging to the HAV, HEV, and NoV GI and GII genogroups) in three different sampling periods (spring, summer, and winter), with the mussels collected from three farms located in the Central Adriatic Sea (Italy). The results showed that 10.42% of the screened animals harbored at least one type of pathogen RNA, more specifically, 5.35% HEV, 4.51% NoV GI, and 0.56% HAV. The highest genetic equivalent (GE) amounts were majorly observed in the winter season (NoV GI 1.0 × 103 GE/g and HEV 1.0 × 102 GE/g), resulting in statistical differences when compared to summer and spring (p-value: <0.001). The original data obtained serve to bring scientific attention to the possible influence of environmental and climatic aspects on viral loads, highlighting the crucial role played by biomolecular assays as preventive medicine tools.

Effect of seasonal changes in temperature on capture efficiency in the blue mussel, Mytilus edulis, fed seston and microplastics

AbstractSuspension‐feeding bivalve molluscs are dominant benthic fauna in many near‐shore environments, with phytoplankton often being their main prey type. Feeding, which involves hydrosol filtration and mucociliary processes to capture particles and process them for digestion, occurs at low Reynolds numbers. Changes in water temperature have been shown to affect feeding processes of bivalves as a result of altered physiological processes or temperature‐dependent changes in kinematic viscosity of water. Most studies, however, have focused on feeding rates and have manipulated temperature under laboratory conditions. In this study, experiments were conducted using ambient seawater and acclimatized blue mussels, Mytilus edulis, to examine particle capture efficiency over a 1‐year period. During this period, water temperature decreased from ~18°C to 5°C with a concomitant increase in viscosity of ~41%. The capture of a wide variety of bacteria and phytoeukaryotes (0.8–8 μm), as well as two sizes of polystyrene microspheres (1 and 6 μm), was quantified to calculate capture efficiency. Data demonstrate that temperature, and the concomitant change in water viscosity, had no significant effect on capture efficiency. Cyanobacteria were captured at significantly higher rates than other bacteria of similar size. These results suggest that the laterofrontal cirri of blue mussels act as paddles rather than sieves because capture efficiency was constant over a range of viscosities and Reynolds numbers. Additionally, the efficient capture of some bacteria and smaller phytoeukaryotes by the mussels suggests that these plankters could play a larger role in the diet of M. edulis than previously considered. The ecological impact of these findings, especially regarding differences in capture efficiency of different bacterial cells, warrants further study.

Analysis of Differential Alternative Splicing in Largemouth Bass After High Temperature Exposure.

Temperature is one of the critical factors affecting the physiological functions of fish. With ongoing global warming, changes in water temperature have a profound impact on fish species. Alternative splicing, being a significant mechanism for gene expression regulation, facilitates fish to adapt and thrive in dynamic and varied aquatic environments. Our study used transcriptome sequencing to analyze alternative splicing in largemouth bass gills at 34 °C for 24 h. The findings indicated an increase in both alternative splicing events and alternative splicing genes after high temperature treatment. Specifically, the comparative analysis revealed a total of 674 differential alternative splicing events and 517 differential alternative splicing genes. Enrichment analysis of differential alternative splicing genes revealed significant associations with various gene ontology (GO) terms and KEGG pathways, particularly in immune-related pathways like necroptosis, apoptosis, and the C-type lectin receptor signaling pathway. These results emphasize that some RNA splicing-related genes are involved in the response of largemouth bass to high temperatures.

Modeling of temperature deformations on the Dnister HPP dam (Ukraine)

Abstract The extended operation of Ukrainian hydroelectric power plants has caused spatial deformations and aging of hydrostructures. This can lead to man-made disasters, especially after explosions resulting from missile attacks. As a result, various defects can appear in the concrete structures of the dam. Continuous structural monitoring of hydropower plants is essential to prevent man-made accidents. The research paper also demonstrates that changes in water temperature can affect the dam’s vertical and horizontal displacements. A model was developed and analysed to determine the spatial deformations of the Dnister HPP based on water temperature and distance from the dam’s edge. It was determined that horizontal displacement rates range from −2.2 to 2.7 mm/month, and vertical displacement rates range from −2 to 1.3 mm/month. They are also seasonal in nature. The studies conducted enable the prediction and identification of seasonal spatial deformations of the Dnister HPP dam. The research also helps to find their variations due to water temperature at the definite depth. If the temperature deformation model deviates from the measurement results, the structure in those areas should be more thoroughly inspected and analysed. This may indicate the structure’s weakening due to natural aging processes or construction-related deficiencies. Deformations in the dam’s structure can lead to cracks, compromising its stability and watertightness. Dams can vary in shape, design features, depth, volume, and water temperature. So, it is important to customize the displacement model for each dam. Any deviation from the specified model may suggest defects due to abnormal temperature distribution and spatial displacements.

Long–period trends in water temperature changes in the northern part of the Atlantic Ocean from ocean reanalysis data

The results of assessing long–period changes in water temperature in the North Atlantic Ocean (0°–70° N, 8°–80° W) based on data from ocean reanalyses and objective analyses for the periods 1961– 2011 and 1980–2011 are presented. The obtained estimates are based on the application of a nonparametric method of regression analysis (quantile regression) to the monthly ocean temperature for a quantile value of 0.5. During the period 1961–2011 warming was mainly observed in the upper 400 m layer in the region from the equator to 70° N. Over this 51-year period, the increase in the median monthly ocean temperature averaged over the analyzed water area ~0.5°C, and in the Gulf Stream–North Atlantic Current system ~1°C. During the period 1980–2011 warming in the North Atlantic Ocean mainly occurred in the upper 1 km layer at high latitudes (50°–65° N). Over this 32-year period, the increase in the median monthly ocean temperature in the subpolar gyre in the upper 400 m layer was ~1°C.

Influence of body size and environmental conditions on parasite assemblages of the black-spotted croaker (Protonibea diacanthus) (Teleostei: Sciaenidae) in northern Australia.

The functioning and richness of marine systems (and biological interactions such as parasitism) are continuously influenced by a changing environment. Using hierarchical modelling of species communities (HMSC), the presence and abundance of multiple parasite species of the black-spotted croaker, Protonibea diacanthus (Sciaenidae), was modelled against environmental measures reflecting seasonal change. Protonibea diacanthus were collected in three seasons across 2019–2021 from four locations within the waters of the Northern Territory, Australia. The length of P. diacanthus proved to have a strong positive effect on the abundance of parasite taxa and overall parasitic assemblage of the sciaenid host. This finding introduces potential implications for parasitism in the future as fish body size responds to fishing pressure and climate changes. Of the various environmental factors measured during the tropical seasons of northern Australia, water temperature and salinity changes were shown as potential causal factors for the variance in parasite presence and abundance, with changes most influential on external parasitic organisms. As environmental factors like ocean temperature and salinity directly affect parasite–host relationships, this study suggests that parasite assemblages and the ecological functions that they perform are likely to change considerably over the coming decades in response to climate change and its proceeding effects.

Simulation of lunar soil compressive strength performance test based on grey correlation analysis

Detecting water ice in the permanent shadow area of the lunar south pole is an important task of China's Chang'e-7. For this purpose, a simulation study was carried out under the earth environment, and a test sample of polar water-containing simulated lunar soil was prepared. The mechanical properties of the lunar soil simulant were tested under ultra-low temperature conditions, and the changes in water content, relative density, temperature, loading rate, and base material ratio with compressive strength were analyzed. The results show that the compressive strength of the frozen lunar soil simulant increases with the increase of water content, increases with the increase of density, and increases with the decrease of temperature. It is less affected by the change of loading rate. The order of compressive strength affected by different base material ratios is: 100%anorthosite, &lt;90%anorthosite, +10%basalt &lt;70%, +30%anorthosite &lt;100%, basalt, lunar soil simulant. Finally, the correlation between the basic physical properties of lunar soil simulant such as water content, relative density, temperature, loading rate, and ratio and compressive strength is explored through grey correlation analysis. The correlation order is: water content&gt; density&gt; temperature&gt; ratio&gt; loading rate. This paper prepared and tested the mechanical properties of hydrated lunar soil, and proposed a more systematic and standardized implementation plan. It serves as the basis for studying the properties of hydrated lunar soil in the polar region, and provides a theoretical basis and reference for subsequent lunar soil simulation research.

The Combined Effects of Temperature and pH to the Toxicity of the Water-Soluble Fraction of Gasoline (WSFG) to the Neotropical Yellow-Tail Tetra, Astyanax altiparanae.

Continental aquatic environments have undergone chemical pollution due to increased anthropogenic activities. Among those substances, petroleum hydrocarbons are a potential hazard for the aquatic animals. Additionally, alterations in the abiotic characteristics of the water, such as temperature and pH, can impose additional stress when those substances are present. We evaluate how alterations in water temperature and pH modified the acute (96h) toxicity of the water-soluble fraction of gasoline (WSFG) to Astyanax altiparanae through physiological analysis. We also investigated the physiological responses after the fish recovery from exposure (96h) in clean water. Both isolated and combined exposures to WSFG resulted in significant physiological changes. Alone, WSFG altered energetic metabolism and haematopoietic functions, potentially due to metabolic hypoxia. When combined with changes in water temperature (30°C) and pH (4.0), A. altiparanae activated additional physiological mechanisms to counterbalance osmoregulatory and acid-base imbalances, likely exacerbated by severe metabolic hypoxia. In both isolated and combined exposure scenarios, A. altiparanae maintained cellular hydration, suggesting a robust capacity to uphold homeostasis under environmental stress conditions. Following a recovery in clean water, energetic metabolism returned to control levels. Nevertheless, plasmatic Na+ and Cl- levels and haematological parameters remained affected by WSFG exposure. Our findings underscore the impact of interactions between WSFG contaminants, temperature and pH, leading to additional biological damage in A. altiparanae.

Future impacts of reduced freshwater inflow and sea level rise on forage fish and their predators in Apalachicola Bay, Florida

Estuarine ecosystems often support large populations of forage fish, but these small-medium sized fish and the predators they feed may be affected by multiple climate change and human-induced stressors. In the Apalachicola Bay estuary, Florida, USA, reduced freshwater inflow and sea level rise are two prevalent stressors, and little is known about how these environmental changes might impact the forage fish species who inhabit the estuary. This study uses a food web model for Apalachicola Bay to simulate the impacts of future changes in water temperature and salinity on forage fish and predator species who inhabit the estuary. Water temperature and salinity conditions were based on previously developed simulations of reduced freshwater inflow and sea level rise in Apalachicola Bay from 2020 to 2049. These simulated environmental changes were applied as forcing functions in the food web model to drive shifts in species biomasses over time. This study centered on changes in biomass for two highly abundant forage fish species, juvenile silver perch (Bairdiella chrysoura) and juvenile pinfish (Lagodon rhomboides), as well as their major predators, spotted seatrout (Cynoscion nebulosus) and inshore lizardfish (Synodus foetens). These changes were assessed at both 10- and 30-year time intervals relative to 2019, along with an examination of forage fish consumption by their predators. Juvenile silver perch biomass increased across all scenarios and time scales, while juvenile pinfish biomass decreased. Consumption of juvenile silver perch by spotted seatrout increased across all scenarios and time scales, and consumption of juvenile pinfish by inshore lizardfish decreased, in concordance with the trends in prey biomass. Both spotted seatrout and inshore lizardfish biomasses decreased across future scenarios, though the high biomass of juvenile silver perch may have played a role in stabilizing the decline of spotted seatrout. Changes in forage fish biomasses and their impacts on predator species as a result of reduced freshwater inflow and sea level rise have implications for ecosystem productivity and the fisheries of the region.

Response of Water Temperature to Wind Speed and Air Temperature in Lake Laut Tawar, Aceh Province, Indonesia

Changes in water temperature impact the dynamics of lake ecosystems. Changing climate factors, including wind speed and air temperature, influence the water temperature of lakes. This research aims to analyze the response of water temperature to wind speed and air temperature in Lake Laut Tawar. Observations were conducted from August to September 2023, with a sampling frequency of every two weeks. The results revealed that water temperature, wind speed, and air temperature in Lake Laut Tawar fluctuated according to the presence of light, namely day and night factors. Variations in sunlight intensity lead to hourly fluctuations in air temperatures, while wind speeds vary hourly due to changes in air pressure, consequently resulting in hourly variations in water temperature as well. During daylight hours, air temperature surpasses water temperature, whereas during nighttime hours, water temperature exceeds air temperature. Heat transfer from the air to the water contributes to an increase in water temperature, while the release of heat energy from the surface water into the air leads to a decrease in water temperature. Changes in the water temperature of Lake Laut Tawar are primarily influenced by changes in wind speed and air temperature by 80 percent simultaneously. However, while air temperature showed a partial response, wind speed did not exhibit a significant response. The relationship between these variables can be expressed through a mathematical model Tw = 0.356 Ta + 0.025 W + 15.674, where Tw is water temperature (°C), Ta is air temperature (°C), and W is wind speed (km/minute). Another factor that influences the water temperature of Lake Laut Tawar is the inlet water temperature, which was not observed in this research.

Relative contribution of the ocean-air heat exchange and advective heat transport to the increase of the Barents Sea water temperature in the early 21st century

The annual water temperature in the major water masses of the Barents Sea (BS) has significantly increased since the early 2000s. Advective heat transport from the neighboring water areas and heat exchange through the sea surface are the major factors, which shape the hydrological conditions in the BS. The paper estimates the contributions of heat exchange at the sea-atmosphere boundary and advective heat transport to changes in the average water temperature of the BS for the entire sea area. The average annual heat balance of the BS is calculated using atmospheric and oceanic reanalysis data. The change in the average temperature of the BS water is estimated taking into account the heat consumption for ice melting. The average surface heat balance from 1993 to 2018 was negative throughout the entire sea area: –70…–100 W/m2 in the south and –10…–20 W/m2 in the north. The advective heat supply was calculated for 9 straits with neighboring water areas. The determining source of advective heat is the influx of Atlantic waters from the Norwegian Sea between Cape Nordkapp and Bear Island. An average of 40.8 TW of advective heat is supplied through this margin. The calculations showed the predominance of annual heat influx due to advection over heat loss from the sea surface. This excess heat influx resulted in an estimated increase in the water temperature of the BS from 1993 to 2018 at a rate of 0.28 °C per year (taking into account the heat consumption for ice melting). In conclusion, it can be argued that the analysis has validated the hypothesis proposed in the article about compensation of heat losses from the surface of the BS by advective heat flow. The hypothesis is quantitatively confirmed by calculations on a simple box model (with an accuracy of up to an order of magnitude) based on atmospheric and oceanic reanalysis data. The ERA5 and GLORYS12V1 reanalysis data reliably describe the basic patterns of observed variability of ocean, sea ice and atmospheric parameters in the Barents Sea.

Variations in deep-sea methane seepage linked to millennial-scale changes in bottom water temperatures ~ 50–6 ka, NW Svalbard margin

During the last glaciation, the northern hemisphere experienced profound millennial-scale changes (termed Dansgaard-Oeschger (DO) events) in atmospheric and oceanic temperatures. In the North Atlantic, the fluctuations resulted in extremely unstable bottom water conditions with bottom water temperatures (BWT) varying up to > 5 °C. We have studied these changes in a core from 1,300 m water depth at Vestnesa Ridge, northwestern Svalbard margin to investigate a possible connection between BWT and seepage of methane from the seafloor covering the period ~ 50–6 ka. Beneath Vestnesa Ridge, gas hydrates containing vast amounts of methane are kept stable due to the high pressure and low temperatures. Release of gas is shown by numerous pockmarks on the seafloor. The pockmarks at 1,300 m water depth are presently inactive, but they bear witness of earlier activity. Our study shows that from ~ 50–6 ka, the core site experienced repeated increases in BWT and in the emissions of gas, both following the pattern of the DO events. This correspondence in time scale indicates that BWT was the primary forcing factor for the variability in methane release. However, the releases were delayed by up to > 1,000 years compared to the initial increase in BWT.

Variations in Temperature and Pressure in the “Reservoir–Well” System Triggered by Blasting Recovery of Iron Ore at the Kursk Magnetic Anomaly

This paper presents the results of precise measurements of temperature and pore pressure in the “reservoir–well” system during the development of iron ore deposits of the Kursk Magnetic Anomaly (KMA) via blasting. For the observation period from October 2021 to June 2024, variations in compressibility, permeability and temperature in the upper Albian-Cenomanian confined aquifer, which is used for district water supply, were determined. The general trend in a decrease in water temperature was traced (from 12 °C to 11.4 °C). It was accompanied by an increase in the hydrostatic head (from 3.7 m to 7.4 m). Water temperature in the upper aquifer was measured for 9 industrial explosions in the mine and for 30 explosions in the quarry. For one explosion in the mine and five explosions in the quarry the coseismic changes in water temperature with amplitudes of 0.06–0.09 °C were established, while changes in pore pressure in the “reservoir–well” system were 0.4–2.2 kPa. Local changes in the permeability of the reservoir in the vicinity of the well (the skin effect) are considered to be the main factor that controls the coseismic response of temperature during industrial explosions. As the reservoir permeability increases, the water temperature in the “reservoir–well” system can decrease and vice versa. The same pattern was observed according to regime measurements performed in 2022–2023. The recorded coseismic responses of water temperature in the upper aquifer in the high-frequency range are similar to the effects observed during propagation of seismic waves originated from earthquakes in the low-frequency range at different sites all over the world for the seismic energy density of 0.05–0.45 J/m3. The observed variations in aquifer temperature in the “reservoir–well” system under episodic dynamic impacts are of particular interest from the point of view of activating hydrogeochemical processes that accompany the development of iron ore deposits.

A novel optimal temporal lag air2stream model using dynamic time series matching

ABSTRACT In order to solve the problem that the current air2stream model does not consider the lag change of water temperature, this paper proposes a new method that combines empirical mode decomposition, power spectral density and dynamic time warping algorithm to determine the optimal time lag parameters, and then constructs the air2stream with the optimal time lag (a2swtl). Finally, the effectiveness and stability of the new method and model were verified based on the measured data from 2020-2022 at three hydrological stations in the middle and lower reaches of the Yangtze River that are close to natural river conditions. The results show that the new method overcomes the problem of low accuracy in determining the time lag of the existing correlation coefficient method. Compared with the original model, a2swtl has better stability without introducing additional observation data, and the average prediction accuracy is improved by about 6.33%. 8.36% and 15.4%.

Transcriptomic Analysis of the Effects of Changes in Temperature and Flow Velocity on Reproductive Ability of Coreius guichenoti

ABSTRACTThe establishment of reservoirs, which alter water temperature and flow dynamics, significantly affects the indigenous fish population, yet the underlying molecular mechanisms remain unclear. To investigate effects of the changing water temperature and flow rate on fish ovaries, we subjected female Coreius guichenoti to the combination of different water temperatures (17°C, 20°C and 23°C denoted as L, N and H, respectively) and flow velocities (0 and 0.5 m/s denoted as S and V, respectively). After 30‐day experiment period, we identified 393, 545, 300 and 657 differentially expressed transcripts in HV versus NV, LV versus NV, HS versus NV and LS versus NV, respectively. The transcriptome results showed that both environmental factors could significantly influence oogenesis, with water temperature exerting a more pronounced effect than flow velocity. In particular, the higher temperature (HV and HS) led to a tendency towards masculinization and even infertility in females, which were consistent with the histopathology results. By contrast, the lower temperature (LV and LS) promoted the progression of desirable female attributes, where static water conditions (LS) had a greater effect compared with flowing water (LV). These findings were of great significance for the adaptive operation of reservoirs to create reasonable and precise ecological flows for managing fish reproduction.

Analysis of operation regulation on delay time in long-distance heating pipe systems for practical engineering

The distribution area of the district heating network (DHN) is extensive, and there are inherent time delays and thermal losses in the process of heat transfer through heating pipes. The delay in heat transfer within long-distance heating pipes may result in inadequate heat supply to end-users or excessive energy consumption at the heat source. Therefore, this paper presents a quasi-dynamic model for calculating the transmission delay time in the long-distance heating pipeline. And the model is validated through the measured values obtained from a heating pipeline. The influencing factors of delay time are further discussed, including operating parameters, pipe structure parameters and thermal insulator thickness. Additionally, the impact of pipe delay time in practical engineering is analyzed. In practical engineering, the transmission delay time varies when the pipe structural or operational parameters differ, even under the same outdoor temperature change. The change in inlet water temperature and mass flow rate can impact the change rate of outlet water temperature, thereby influencing the delay time. Furthermore, the delay time exhibited an increase with pipe length, diameter, and thermal insulator thickness; however, the effect of thermal insulator thickness on it was minimal. When the inlet water temperature rose or dropped by 5℃, the delay time grew by more 70 % per 1 km pipe length, about 40 % per 100 mm diameter and less 2 % per 100 mm thermal insulator thickness, respectively.