Changes In Transport Capacity Research Articles

Predicting soil erosion and deposition on sloping farmland with different shapes in northeast China by using 137Cs

Slope shape has an important influence on soil erosion. However, the distribution of erosion and deposition on sloping farmland with different shapes is still unclear. This severely restricts the soil loss evaluation and protection of Mollisol resources in northeast China, where sloping farmland (<10°) accounts for 60 % of the cultivated land. In this study, the 137Cs technique combined with wavelet analysis theory was employed to study the spatial distribution characteristics of erosion–deposition rates along three slopes with straight, convex, and concave shapes in northeast China. The results indicated that the average erosion rates of the three slopes were in the following order: convex > straight > concave. The soil erosion and deposition rates fluctuated along the slope length. The primary fluctuation periods in the straight slope, convex slope and concave slope were 45.1 m, 55.0 m, and 40.4 m, respectively. Three relationships of coupled effects of slope gradient and slope length on straight, convex and concave slope were established and verified. It can be concluded that the relationship between the soil erosion rate and slope length was not simple monotonous but showed a certain periodic fluctuation, which was related to the change in transport capacity of runoff on the slope. The change in the erosion–deposition rate with slope length in a straight slope shape can be described by the cosine function. The cumulative soil erosion–deposition rates along the slope had very significant binary power function relationships with both slope length and gradient on convex and concave slopes. The coupled effects of slope gradient and length on soil erosion–deposition for different slope shapes should be fully considered for soil erosion prediction. This study could provide theoretical support for improving erosion prediction models and a scientific basis for the rational arrangement of soil and water conservation measures on slopes at appropriate locations.

AMS and rock magnetism in the Caviahue-Copahue Volcanic Complex (Southern Andes): Emission center, flow dynamics, and implications to the emplacement of non-welded PDCs

Pyroclastic deposits can cover significant areas and register major geological events. Despite their importance, understanding depositional dynamics of pyroclastic density currents (PDCs) and linking explosive deposits to their emission centers is still a challenge, especially in the case of non-welded, massive ignimbrites. Located in the Southern Andes, the Caviahue Copahue Volcanic Complex (CCVC) comprises one of the most active volcanic centers in the Andean Belt. This volcanic complex hosts massive ignimbrites with both source emplacement poorly constrained, currently grouped in the Riscos Bayos Ignimbrites (RBI). In this contribution, we perform a full magnetic characterization and anisotropy of magnetic susceptibility (AMS) study on the massive RBI of the CCVC. The magnetic characterization was performed using magnetic experiments including isothermal remanet magnetization, thermomagnetic curves, hysteresis loops, first-order reversal curves, and scanning electron microscopy. Magnetic experiments indicate primary, multi-domain, high Curie temperature titanomagnetites as the AMS carriers. Ellipsoids are predominately oblate, with a low degree of anisotropy and east-southeastward imbrication. This fabric arrangement is consistent with PDC sedimentary fabrics deposited under laminar flow conditions. Despite RBI massive structure AMS data reveals changes in transport capacity of the PDC and particle organization. These changes are marked by increasing AMS dispersion and decreasing degree of anisotropy up-section within flow units. Directional statistics of AMS data implies the Las Mellizas Caldera as the emission center of RBI. The reconstructed flow path also suggests the PDC overrun of the Caviahue Caldera topographic rim. This study highlights the application of AMS to the identification of emission centers of explosive deposits, featuring its application to massive ignimbrites.

Transport and storage of bed material in a gravel‐bed channel during episodes of aggradation and degradation: a field and flume study

ABSTRACTThe dynamics of sediment transport capacity in gravel‐bed rivers is critical to understanding the formation and preservation of fluvial landforms and formulating sediment‐routing models in drainage systems. We examine transport‐storage relations during cycles of aggradation and degradation by augmenting observations of three events of channel aggradation and degradation in Cuneo Creek, a steep (3%) gravel‐bed channel in northern California, with measurements from a series of flume runs modeling those events. An armored, single‐thread channel was formed before feed rates were increased in each aggradation run. Output rates increased as the channel became finer and later widened, steepened, and braided. After feed rates were cut, output rates remained high or increased in early stages of degradation as the incising channel remained fine‐grained, and later decreased as armoring intensified. If equilibrium was not reached before sediment feed rate was cut, then a rapid transition from a braided channel to a single‐thread channel caused output rates for a given storage volume to be higher during degradation than during aggradation. Variations in channel morphology, and surface bed texture during runs that modeled the three cycles of aggradation and degradation were similar to those observed in Cuneo Creek and provide confidence in interpretations of the history of change: Cuneo Creek aggraded rapidly as it widened, shallowed, and braided, then degraded rapidly before armoring stabilized the channel. Such morphology‐driven changes in transport capacity may explain the formation of flood terraces in proximal channels. Transport‐storage relations can be expected to vary between aggradation and degradation and be influenced by channel conditions at the onset of changes in sediment supply. Published in 2011. This article is a US Government work and is in the public domain in the USA.

Zinc transport mediated by barley ZIP proteins are induced by low pH

It is estimated that nearly 50% of the world’s population is at risk of zinc (Zn) deficiency. The challenge is therefore to increase the Zn content in edible plant parts in order to improve the nutritional value of staple foods. We recently reported the identification and characterization of three barley genes encoding zinc transport proteins belonging to the ZIP protein family. These proteins are believed to be involved in cellular uptake of Zn2+. In this addendum, the Zn2+ transport capacity of ZIP proteins isolated from barley roots was investigated in response to various pH levels. We show that a lowering of pH induces a better growth at low Zn2+ concentrations of yeast cells expressing ZIP proteins.. However, no significant change in transport capacity (Vmax) could be observed for HvIRT1, whereas lowering of pH from 5.5 to 4.2 increased the Vmax value with 64% for HvZIP5. These results indicate that proton activity has an important role in regulating the Zn2+ transport capacity of Zn2+ specific ZIP transport proteins. This information will increase the understanding of ZIP proteins and facilitate engineering of genotypes able to grow efficiently on marginal soils.

Hormonal control of salt and water balance in vertebrates

The endocrine system mediates many of the physiological responses to the homeostatic and acclimation demands of salt and water transport. Many of the hormones involved in the control of salt and water transport are common to all vertebrates, although their precise function and target tissues have changed during evolution. Arginine vasopressin (vasotocin), angiotensin II, natriuretic peptides, vasoactive intestinal peptide, urotensin II, insulin and non-genomic actions of corticosteroids are involved in acute (minutes and hours) alterations in ion and water transport. This rapid alteration in transport is primarily the result changes in behavior, blood flow to osmoregulatory organs, and membrane insertion or activation (e.g., phosphorylation) of existing transport proteins, ion and water channels, contransporters and pumps. Corticosteroids (through genomic actions), prolactin, growth hormone, and insulin-like growth factor I primarily control long-term (several hours to days) changes in transport capacity that are the result of synthesis of new transport proteins, cell proliferation, and differentiation. In addition to the important task of establishing broad evolutionary patterns in hormones involved in ion regulation, comparative endocrinology can determine species and population level differences in signaling pathways that may be critical for adaptation to extreme or rapidly changing environments.

Characterization of regulatory mechanisms and states of human organic cation transporter 2

Polyspecific organic cation transporters (OCTs) have a large substrate binding pocket with different interaction domains. To determine whether OCT regulation is substrate specific, suitable fluorescent organic cations were selected by comparing their uptake in wild-type (WT) human embryonic kidney (HEK)-293 cells and in HEK-293 cells stably transfected with hOCT2. N-amidino-3,5-diamino-6-chloropyrazine-carboxamide (amiloride) and 4-[4-(dimethylamino)-styryl]-N-methylpyridinium (ASP) showed concentration-dependent uptake in hOCT2 at 37 degrees C. After subtraction of unspecific uptake determined in WT at 37 degrees C or in hOCT2 at 8 degrees C saturable specific uptake of both substrates was measured. Km values of hOCT2-mediated uptake of 95 microM amiloride and 24 microM ASP were calculated. Inhibition of amiloride and ASP uptake by several organic cations was also measured [IC50 (in microM) for amiloride and ASP, respectively, tetraethylammonium (TEA) 98 and 30, cimetidine 14 and 26, and tetrapentylammonium (TPA) 7 and 2]. Amiloride and ASP uptake were significantly reduced by inhibition of Ca2+/CaM complex (-55 +/- 5%, n = 10 and -63 +/- 2%, n = 15, for amiloride and ASP, respectively) and stimulation of PKC (-54 +/- 5%, n = 14, and -31 +/- 6%, n = 26) and PKA (-16 +/- 5%, n = 16, and -18 +/- 4%, n = 40), and they were increased by inhibition of phosphatidylinositol 3-kinase (+28 +/- 6%, n = 8, and +55 +/- 17%, n = 16). Inhibition of Ca2+/CaM complex resulted in a significant decrease of Vmax (160-99 photons/s) that can be explained in part by a reduction of the membrane-associated hOCT2 (-22 +/- 6%, n = 9) as determined using FACScan flow cytometry. The data indicate that saturable transport by hOCT2 can be measured by the fluorescent substrates amiloride and ASP and that transport activity for both substrates is regulated similarly. Inhibition of the Ca2+/CaM complex causes changes in transport capacity via hOCT2 trafficking.

Regulation of functional nuclear pore size in fibroblasts.

Protein-NLS-coated gold particles up to approximately 250 A in diameter are transported through the nuclear pores in normal, proliferating BALB/c 3T3 cells. This size can increase or decrease, depending on cellular activity. It has been suggested that increases in functional pore size are related to a reduction in the amount of available p53. To further test this hypothesis, we investigated the effects of cycloheximide and pifithrin-alpha, which inhibits p53-dependent transcriptional activation, on nuclear transport. After 3 hours in cycloheximide, there was a significant increase in the size of the gold particles that entered the nucleoplasm. When the incubation period was extended to 6 hours or longer, transport capacity returned to the control level. By using proteasome inhibitors, it was shown that the cycloheximide-dependent increase in functional pore size was due to the inhibition of protein synthesis, consistent with the fact that p53 is a short-lived protein, and requires the activity of at least two different factors. Although cycloheximide increases the functional diameter of the channel available for signal-mediated transport by approximately 60 A, it had no significant effect on either the import rate of small NLS-containing substrates (FITC-BSA-NLS), or passive diffusion of fluorescent-labeled proteins across the envelope. This suggests that changes in transport capacity were not caused by an increase in overall pore diameter but instead are due to a transient increase in pore size that accompanies signal-mediated transport. Pifithrin-alpha also caused an increase in functional pore diameter without altering the import rate of FITC-BSA-NLS, providing further support for the view that p53 can initiate changes in nuclear transport capacity.

Posttranscriptional regulation of the proximal tubule NaPi-II transporter in response to PTH and dietary P(i).

The rate of proximal tubular reabsorption of phosphate (P(i)) is a major determinant of P(i) homeostasis. Deviations of the extracellular concentration of P(i) are corrected by many factors that control the activity of Na-P(i) cotransport across the apical membrane. In this review, we describe the regulation of proximal tubule P(i) reabsorption via one particular Na-P(i) cotransporter (the type IIa cotransporter) by parathyroid hormone (PTH) and dietary phosphate intake. Available data indicate that both factors determine the net amount of type IIa protein residing in the apical membrane. The resulting change in transport capacity is a function of both the rate of cotransporter insertion and internalization. The latter process is most likely regulated by PTH and dietary P(i) and is considered irreversible since internalized type IIa Na-P(i) cotransporters are subsequently routed to the lysosomes for degradation.

Lactate/H+ transport kinetics in rat skeletal muscle related to fibre type and changes in transport capacity.

Lactate/H+ transport kinetics were determined by means of the pH-sensitive probe BCECF in sarcolemmal giant vesicles, obtained from rat skeletal muscle, and related to variations in lactate/H+ transport capacity. Vesicle preparations were made from red and white muscles, mixed muscles, denervated muscles, muscles of old rats and rats that had been subjected to high-intensity training, endurance training, repeated exposure to hypoxia, and hypothyroid or hyperthyroid treatments. The lactate/H+ transport capacity of red muscles was greater than that of white muscles, and this difference was associated with a higher maximal transport rate (V max) in red muscles, whereas the K m was similar in the two muscle types. High-intensity training and hyperthyroidism increased the lactate/H+ transport capacity by enhancing V max without affecting K m. Similarly, a reduced transport capacity with old age and hypothyroidism was due to a decrease in V max. The denervation-induced decline in lactate/H+ transport capacity resulted from both an increased K m and a reduced V max. The present data show that muscle type differences and most changes in the lactate/H+ transport capacity are mediated by modifications in V max, which is expected to represent the number of membrane transporter molecules. K m is unaffected by most treatments and appears to be independent of fibre type.

Variations in Signal-Mediated Nuclear Transport during the Cell Cycle in BALB/c 3T3 Cells

Signal-mediated nuclear import was investigated in proliferating BALB/c 3T3 cells 1, 2, 4, 6, 8, 12, 18, and 21 h after the onset of anaphase. Using nucleoplasmin-coated colloidal gold particles to assay transport capacity, it was found that import was greatest at 1 h postanaphase (after complete reformation of the nuclear envelope). At this time, both the relative rate of gold uptake and the functional size of the transport channels were significantly greater than in control cells, which were randomly selected from nonsynchronized cultures. At 21 b, there was a decrease in the size of the transport channel, but not the rate of nuclear import. This suggests that these two factors are regulated independently. No changes in transport capacity, compared to controls, were detected at other times in the cell cycle.

Actions of sex steroids and corticosteroids on rabbit choroid plexus as shown by changes in transport capacity and rate of cerebrospinal fluid formation

The effect of betamethasone on choroid plexus transport and CSF formation in rabbits was studied. Following 5 days of daily treatment with betamethasone the CSF production rate was reduced by 43% as measured by ventriculo-cisternal perfusion with radioactive inulin. Accordingly, the Na(+)-K(+)-ATPase activity and the transport capacity in the choroid plexus, measured in terms of choline (10(-5) M) uptake and accumulation in vitro, decreased (in the lateral ventricles by 31% in both cases). Isolated choroid plexuses from rabbits were also used to determine uptake and accumulation of choline and the activities of various types of ATPases following pretreatment of the animals with 17-beta-oestradiol, alone or in combination with progesterone. The combined treatment reduced the choline uptake by 35% and also lowered the activity of Na(+)-K(+)-ATPase by 31% without influencing tissue wet weight. Thus, the demonstrated influences of glucocorticoids and sex steroids on the transport capacity in the choroid plexus seem to be important components in their postulated effects on intracranial hypertension.

Germination of Wheat Embryos and the Transport of Amino Acids into a Protein Synthesis Precursor Pool

Wheat (Triticum aestivum L. var. Lew) embryonic axes take up externally supplied radioactive amino acid (from a solution greater than 2 millimolar) such that the specific radioactivity of the total internal amino acid rapidly reaches that of the external solution. Nevertheless, incorporation of radioactive amino acid into protein increases steadily as the concentration of external amino acid is increased, indicating that the amino acid that is precursor to protein synthesis is not in equilibrium with the total internal amino acid pool. When the external source of amino acid is removed, incorporation of radiolabeled amino acid into protein continues at a rate comparable to that of embryos maintained in the radioactive solution. In explanation of these data, it is suggested that there are two separate cytoplasmic pools of amino acids, one a protein synthesis precursor pool, and the second, an expandable pool into which exogenous radioactive amino acids are taken up. The protein synthesis pool is fed at a limited rate from the expandable pool and at a far greater rate from an endogenous source. As a consequence, the specific activity of the amino acid that is the precursor for protein synthesis is considerably below that of the total internal pool and is determined by the rate of movement into the protein synthesis pool from the expanded radioactive cytoplasmic pool.The rate of movement of amino acids from the expandable pool into the protein synthesis pool increases approximately 5-fold during the initial 4.5 hours of embryo germination. When this change is considered in analyzing the relative rates of protein synthesis, there is probably no more than a 2-fold increase in protein synthetic capacity between embryos germinated for 1.5 and 4.5 hours. The leveling off of the change in transport capacity after 4.5 hours suggests that the earlier increase in the rate of this process may be a necessary step before the embryos can begin to accelerate their growth rate.