Changes In Segment Length Research Articles

How to treat mixed behavior segments in supervised machine learning of behavioural modes from inertial measurement data

The application of supervised machine learning methods to identify behavioural modes from inertial measurements of bio-loggers has become a standard tool in behavioural ecology. Several design choices can affect the accuracy of identifying the behavioural modes. One such choice is the inclusion or exclusion of segments consisting of more than a single behaviour (mixed segments) in the machine learning model training data. Currently, the common practice is to ignore such segments during model training. In this paper we tested the hypothesis that including mixed segments in model training will improve accuracy, as the model would perform better in identifying them in the test data. We test this hypothesis using a series of data simulations on four datasets of accelerometer data coupled with behaviour observations, obtained from four study species (Damaraland mole-rats, meerkats, olive baboons, polar bears). Results show that when a substantial proportion of the test data are mixed behaviour segments (above ~ 10%), including mixed segments in machine learning model training improves the accuracy of classification. These results were consistent across the four study species, and robust to changes in segment length, sample size, and degree of mixture within the mixed segments. However, we also find that in some cases (particularly in baboons) models trained with mixed segments show reduced accuracy in classifying test data containing only single behaviour (pure) segments, compared to models trained without mixed segments. Based on these results, we recommend that when the classification model is expected to deal with a substantial proportion of mixed behaviour segments (> 10%), it is beneficial to include them in model training, otherwise, it is unnecessary but also not harmful. The exception is when there is a basis to assume that the training data contains a higher rate of mixed segments than the actual (unobserved) data to be classified—such a situation may occur particularly when training data are collected in captivity and used to classify data from the wild. In this case, excess inclusion of mixed segments in training data should probably be avoided.

Inhibitory synaptic transmission is impaired in the Kölliker-Fuse of male, but not female, Rett syndrome mice.

Rett syndrome (RTT) is a severe neurodevelopmental disorder that mainly affects females due to silencing mutations in the X-linked MECP2 gene. One of the most troubling symptoms of RTT is breathing irregularity, including apneas, breath-holds, and hyperventilation. Mice with silencing mutations in Mecp2 exhibit breathing abnormalities similar to human patients and serve as useful models for studying mechanisms underlying breathing problems in RTT. Previous work implicated the pontine, respiratory-controlling Kölliker-Fuse (KF) in the breathing problems in RTT. The goal of this study was to test the hypothesis that inhibitory synaptic transmission is deficient in KF neurons from symptomatic male and female RTT mice. We performed whole cell voltage-clamp recordings from KF neurons in acute brain slices to examine spontaneous and electrically evoked inhibitory post-synaptic currents (IPSCs) in RTT mice and age- and sex-matched wild-type mice. The frequency of spontaneous IPSCs was reduced in KF neurons from male RTT mice but surprisingly not in female RTT mice. In addition, electrically evoked IPSCs were less reliable in KF neurons from male, but not female, RTT mice, which was positively correlated with paired-pulse facilitation, indicating decreased probability of release. KF neurons from male RTT mice were also more excitable and exhibited shorter-duration action potentials. Increased excitability of KF neurons from male mice was not explained by changes in axon initial segment length. These findings indicate impaired inhibitory neurotransmission and increased excitability of KF neurons in male but not female RTT mice and suggest that sex-dependent mechanisms contribute to breathing problems in RTT.NEW & NOTEWORTHY Kölliker-Fuse (KF) neurons in acute brain slices from male Rett syndrome (RTT) mice receive reduced inhibitory synaptic inputs compared with wild-type littermates. In female RTT mice, inhibitory transmission was not different in KF neurons compared with controls. The results from this study show that sex-specific alterations in synaptic transmission occur in the KF of RTT mice.

Anatomical Correlates of Cursoriality are Compromised by Body Size and Propensity to Burrow in a Group of Small Mammals (Lagomorpha)

Highly cursorial animals are specialised for fast, sustained running via specific morphological adaptations, notably including changes in limb segment length and mechanical advantage. Members of the order Lagomorpha (hares, rabbits and pikas) vary in cursorial ability; hares are generally highly cursorial, rabbits more frequently saltate, and pikas predominantly trot. Previous investigations of lagomorphs have identified anatomical trends correlated with this ‘cursoriality gradient’, however, the phylogenetic sampling of such investigations has been limited to three American species, namely the American pika (Ochotona princeps), brush rabbit (Sylvilagus bachmani), and black-tailed jackrabbit (Lepus californicus). Here, we expand the phylogenetic sample and body size range by including novel data from Australian samples of the European rabbit (Oryctolagus cuniculus) and European hare (L. europaeus), alongside unpublished data on the Eastern cottontail (S. floridanus). X-ray Computed Tomography and digital landmarking were used to capture proportions within the appendicular skeleton of ~ 40 specimens of each European species. In doubling the number of species studied, we find the previously-identified morphological gradients associated with cursorial behaviour are complicated when evaluated in the larger sample. The relative length and joint velocity of limbs was found to be lower than predicted in European rabbits and hares. Furthermore, we present a novel assessment of morphological integration in the lagomorph appendicular skeleton, finding between-limb covariation patterns that are generally similar to those of other mammals. Broadly, these results suggest cursoriality is only one of many selective forces driving lagomorph skeletal evolution, with variations in body size and fossoriality potentially having measurable impacts.

Influence of the Analytical Segment Length on the Tram Track Quality Assessment

In the track quality analysis, numerical values representing the relative condition of track geometry called track quality indices (TQIs) are calculated along a specific track segment. Segments are defined as linear track geometry datasets with the homogeneous characteristics of factors affecting geometry degradation. The 200m-long analytical segment is used most often on inter-city conventional and high-speed rail networks. However, in the case of the small urban rail networks, the homogeneity of track-geometry degradation influential factors is very low. This segment length is usually too long for efficient track maintenance or reconstruction with minimal disruption of the urban traffic. This paper explores the effect of reducing the analytical segment length in the condition assessment of the tram network in the City of Osijek, Croatia. The research had two main objectives: (1) to assess the narrow-gauge tram-track geometry quality through the application of the established synthesized TQIs, and (2) to analyze how a change in the analytical segment length affects this assessment. Two synthesized track quality indices—one based on a weighted value and the other on a standard deviation of measured track geometry parameters—were calculated for the 27.5 km of tracks on consecutive 200-, 100-, 50-, and 25 m long analytical segments. The comparative analysis of the TQIs’ calculation results showed that the reduction in the segment length increased the resolution of the track quality analysis in both cases, while the index based on a weighted value of geometry deviations proved less sensitive to this reduction. These results contribute to further segmentation process establishment and TQIs implementation on tram infrastructure.

Human cone elongation responses can be explained by photoactivated cone opsin and membrane swelling and osmotic response to phosphate produced by RGS9-catalyzed GTPase

Human cone outer segment (COS) length changes in response to stimuli bleaching up to 99% of L- and M-cone opsins were measured with high resolution, phase-resolved optical coherence tomography (OCT). Responses comprised a fast phase (∼5 ms), during which COSs shrink, and two slower phases (1.5 s), during which COSs elongate. The slower components saturated in amplitude (∼425 nm) and initial rate (∼3 nm ms-1) and are well described over the 200-fold bleaching range as the sum of two exponentially rising functions with time constants of 80 to 90 ms (component 1) and 1,000 to 1,250 ms (component 2). Measurements with adaptive optics reflection densitometry revealed component 2 to be linearly related to cone pigment bleaching, and the hypothesis is proposed that it arises from cone opsin and disk membrane swelling triggered by isomerization and rate-limited by chromophore hydrolysis and its reduction to membrane-localized all-trans retinol. The light sensitivity and kinetics of component 1 suggested that the underlying mechanism is an osmotic response to an amplified soluble by-product of phototransduction. The hypotheses that component 1 corresponds to G-protein subunits dissociating from the membrane, metabolites of cyclic guanosine monophosphate (cGMP) hydrolysis, or by-products of activated guanylate cyclase are rejected, while the hypothesis that it corresponds to phosphate produced by regulator of G-protein signaling 9 (RGS9)-catalyzed hydrolysis of guanosine triphosphate (GTP) in G protein-phosphodiesterase complexes was found to be consistent with the results. These results provide a basis for the assessment with optoretinography of phototransduction in individual cone photoreceptors in health and during disease progression and therapeutic interventions.

Combining Image Restoration and Traction Force Microscopy to Study Extracellular Matrix-Dependent Keratin Filament Network Plasticity.

Keratin intermediate filaments are dynamic cytoskeletal components that are responsible for tuning the mechanical properties of epithelial tissues. Although it is known that keratin filaments (KFs) are able to sense and respond to changes in the physicochemical properties of the local niche, a direct correlation of the dynamic three-dimensional network structure at the single filament level with the microenvironment has not been possible. Using conventional approaches, we find that keratin flow rates are dependent on extracellular matrix (ECM) composition but are unable to resolve KF network organization at the single filament level in relation to force patterns. We therefore developed a novel method that combines a machine learning-based image restoration technique and traction force microscopy to decipher the fine details of KF network properties in living cells grown on defined ECM patterns. Our approach utilizes Content-Aware Image Restoration (CARE) to enhance the temporal resolution of confocal fluorescence microscopy by at least five fold while preserving the spatial resolution required for accurate extraction of KF network structure at the single KF/KF bundle level. The restored images are used to segment the KF network, allowing numerical analyses of its local properties. We show that these tools can be used to study the impact of ECM composition and local mechanical perturbations on KF network properties and corresponding traction force patterns in size-controlled keratinocyte assemblies. We were thus able to detect increased curvature but not length of KFs on laminin-322 versus fibronectin. Photoablation of single cells in microprinted circular quadruplets revealed surprisingly little but still significant changes in KF segment length and curvature that were paralleled by an overall reduction in traction forces without affecting global network orientation in the modified cell groups irrespective of the ECM coating. Single cell analyses furthermore revealed differential responses to the photoablation that were less pronounced on laminin-332 than on fibronectin. The obtained results illustrate the feasibility of combining multiple techniques for multimodal monitoring and thereby provide, for the first time, a direct comparison between the changes in KF network organization at the single filament level and local force distribution in defined paradigms.

Direct in vivo assessment of global and regional mechanoelectric feedback in the intact human heart

BackgroundInhomogeneity of ventricular contraction is associated with sudden cardiac death, but the underlying mechanisms are unclear. Alterations in cardiac contraction impact electrophysiological parameters through mechanoelectric feedback. This has been shown to promote arrhythmias in experimental studies, but its effect in the in vivo human heart is unclear.ObjectiveThe purpose of this study was to quantify the impact of regional myocardial deformation provoked by a sudden increase in ventricular loading (aortic occlusion) on human cardiac electrophysiology.MethodsIn 10 patients undergoing open heart cardiac surgery, left ventricular (LV) afterload was modified by transient aortic occlusion. Simultaneous assessment of whole-heart electrophysiology and LV deformation was performed using an epicardial sock (240 electrodes) and speckle-tracking transesophageal echocardiography. Parameters were matched to 6 American Heart Association LV model segments. The association between changes in regional myocardial segment length and activation-recovery interval (ARI; a conventional surrogate for action potential duration) was studied using mixed-effect models.ResultsIncreased ventricular loading reduced longitudinal shortening (P = .01) and shortened ARI (P = .02), but changes were heterogeneous between cardiac segments. Increased regional longitudinal shortening was associated with ARI shortening (effect size 0.20 [0.01–0.38] ms/%; P = .04) and increased local ARI dispersion (effect size –0.13 [–0.23 to –0.03] ms/%; P = .04). At the whole organ level, increased mechanical dispersion translated into increased dispersion of repolarization (correlation coefficient r = 0.81; P = .01).ConclusionMechanoelectric feedback can establish a potentially proarrhythmic substrate in the human heart and should be considered to advance our understanding and prevention of cardiac arrhythmias.

Cervical Spine Musculotendon Lengths When Reading a Tablet in Three Seated Positions.

A popular posture for using wireless technology is reclined sitting, with the trunk rotated posteriorly to the hips. This position decreases the head's gravitational moment; however, the head angle relative to the trunk is similar to that of upright sitting when using a tablet in the lap. This study compared cervical extensor musculotendon length changes from neutral among 3 common sitting postures and maximum neck flexion while using a tablet. Twenty-one participants had radiographs taken in neutral, full-flexion, and upright, semireclined, and reclined postures with a tablet in their lap. A biomechanical model was used to calculate subject-specific normalized musculotendon lengths for 27 cervical musculotendon segments. The lower cervical spine was more flexed during reclined sitting, but the skull was more flexed during upright sitting. Normalized musculotendon length increased in the reclined compared with an upright sitting position for the C4-C6/7 (deep) and C2-C6/7 (superficial) multifidi, semispinalis cervicis (C2-C7), and splenius capitis (Skull-C7). The suboccipital (R2 = .19-.71) and semispinalis capitis segment length changes were significantly correlated with the Skull-C1 angle (0.24-0.51). A semireclined reading position may be an ideal sitting posture to reduce the head's gravitational moment arm without overstretching the assessed muscles.

Modern treatment approaches in patients with false joints and lower extremities long bones defects: analytical review

Research of effective treatment methods in patients with pseudoarthrosis and lower extremities long bones defects remains today very relevant. In the structure of traumatological patients general disability it takes up to 33.1% of cases. Surgical techniques are used, such as: intramedullary osteosynthesis, method of open reposition and bone fragments fixation by metal structures, method of transcutaneous osteosynthesis. They are recognized as effective treatment methods in patients with pseudoarthrosis and lower extremities long bones defects, that greatly improves treatment outcomes. Unfortunately, when they are used as single intervention, unsatisfactory therapy results occur in up to half of the cases. Concomitant structural and functional changes of the limb - such as regional blood circulation insufficiency, presence of extensive cicatrical soft tissues defects, changes in bone segment length, joints contractures, etc. - interferes bone consolidation and requires an application of additional treatment methods. Thus, the generally accepted treatment approach in patients with pseudoarthrosis and lower extremities long bones defects is a combination of surgery, pharmacotherapy, methods of physical therapy and physiotherapy in order to optimize the processes of osteoregeneration in non-union area.

Digital Image Correlation Systems in the Experimental Investigations: Capabilities and Limitations

AbstractThe aim of the paper is to present the possibilities and limitations of using the Digital Image Correlation systems. In order to assess the measurement inaccuracies the measuring volume 1250 × 1100 mm was analysed using two cameras with sensor resolution 6 megapixels. It was stated very good accuracy of the line segment length change. It causes that observation of crack widths can be considered as precisely. Some practical information concern how determine the compatibility between crack width measured traditionally and by using DIC are given. In the second part of the paper the results of the tests concerning capacity of interface between two concrete casting at the same time were presented. Use of the optical measurement system Aramis enables the analysis of the deformation, determination of failure mode of the tested specimens and limit displacement between edges of the interface.

Flexible and coatable insulating silica aerogel/polyurethane composites via soft segment control

We herein propose a facile approach to fabricate foldable and coatable silica aerogel polyurethane composites (APCs). For the flexibility control, the soft segment of polyurethane (PU) is manipulated. The change in soft segment length induces a difference in overall glass transition temperature of PU. When the silica aerogel content is increased, the PU with a shorter soft segment length shows brittle fracture behavior, while the PU with a longer soft segment length shows no breakage after bending. The thermal insulation properties of APCs were enhanced by 72% reduction in thermal conductivity upon 30 wt% aerogel loading and theoretically verified by a micromechanics-based thermal conductivity model considering the effects of interface and agglomeration. In addition, for the combustion behavior measurements, heat release rate and heat release capacity are substantially reduced for the APCs compared to neat PUs. Those enhanced thermal insulation properties may have a commercial impact on thermal insulation applications.

Associations of temporal changes in cervical length and lower uterine segment length with spontaneous preterm delivery risk: a prospective study of 727 Japanese women.

A prospective assessment of the risk of spontaneous preterm delivery (sPTD) by evaluating temporal changes in cervical measurements. We analyzed clinical variables, focusing on cervical length (CL) and lower uterine segment (LUS) length (LUSL) as measured by transvaginal ultrasonography in 727 pregnant Japanese women. In women undergoing term deliveries, CL increased from gestational week (GW) 8-25. In contrast, the combination of CL and LUSL (ComL for "combined length") gradually decreased and sole LUSL became almost 0mm by GW 25. Univariate logistic regression analysis suggested that a history of PTD was a risk factor for sPTD. CL, LUSL, and ComL were not significant predictors of sPTD. To assess the risk of sPTD in the second trimester, it is not necessary to distinguish the cervix from the LUS.

Quasi-Distributed Active-Mode-Locking Laser Interrogation with Multiple Partially Reflecting Segment Sensors.

A new type of quasi-distributed sensor system is implemented using an active mode locking (AML) laser cavity with multiple partially reflecting segments. The mode locking frequency of the AML laser is linearly proportional to the overall lasing cavity length. To implement multiple resonators having multiple reflection points installed in a sensing fiber, two types of partial reflectors (PRs) are implemented for an in-line configuration, one with fiber Bragg grating and the other with a fiber Fabry–Perot interferometer. Since the laser has oscillated only when the modulation frequencies for the mode locking frequency match with the corresponding resonator lengths, it is possible to read the multiple partially reflecting segments along the sensing fiber. The difference between two corresponding mode locking frequencies is changing proportionally with the segment length variation between two PRs upon strain application. The segment length change caused by the applied strain can be successfully measured with a linear sensitivity between mode locking frequency and displacement, linearity over 0.99, and spatial position resolution below meter order.

Sensor-Motor Maps for Describing Linear Reflex Composition in Hopping.

In human and animal motor control several sensory organs contribute to a network of sensory pathways modulating the motion depending on the task and the phase of execution to generate daily motor tasks such as locomotion. To better understand the individual and joint contribution of reflex pathways in locomotor tasks, we developed a neuromuscular model that describes hopping movements. In this model, we consider the influence of proprioceptive length (LFB), velocity (VFB) and force feedback (FFB) pathways of a leg extensor muscle on hopping stability, performance and efficiency (metabolic effort). Therefore, we explore the space describing the blending of the monosynaptic reflex pathway gains. We call this reflex parameter space a sensor-motor map. The sensor-motor maps are used to visualize the functional contribution of sensory pathways in multisensory integration. We further evaluate the robustness of these sensor-motor maps to changes in tendon elasticity, body mass, segment length and ground compliance. The model predicted that different reflex pathway compositions selectively optimize specific hopping characteristics (e.g., performance and efficiency). Both FFB and LFB were pathways that enable hopping. FFB resulted in the largest hopping heights, LFB enhanced hopping efficiency and VFB had the ability to disable hopping. For the tested case, the topology of the sensor-motor maps as well as the location of functionally optimal compositions were invariant to changes in system designs (tendon elasticity, body mass, segment length) or environmental parameters (ground compliance). Our results indicate that different feedback pathway compositions may serve different functional roles. The topology of the sensor-motor map was predicted to be robust against changes in the mechanical system design indicating that the reflex system can use different morphological designs, which does not apply for most robotic systems (for which the control often follows a specific design). Consequently, variations in body mechanics are permitted with consistent compositions of sensory feedback pathways. Given the variability in human body morphology, such variations are highly relevant for human motor control.

Strain analysis in CRT candidates using the novel segment length in cine (SLICE) post-processing technique on standard CMR cine images

ObjectivesAlthough myocardial strain analysis is a potential tool to improve patient selection for cardiac resynchronization therapy (CRT), there is currently no validated clinical approach to derive segmental strains. We evaluated the novel segment length in cine (SLICE) technique to derive segmental strains from standard cardiovascular MR (CMR) cine images in CRT candidates.MethodsTwenty-seven patients with left bundle branch block underwent CMR examination including cine imaging and myocardial tagging (CMR-TAG). SLICE was performed by measuring segment length between anatomical landmarks throughout all phases on short-axis cines. This measure of frame-to-frame segment length change was compared to CMR-TAG circumferential strain measurements. Subsequently, conventional markers of CRT response were calculated.ResultsSegmental strains showed good to excellent agreement between SLICE and CMR-TAG (septum strain, intraclass correlation coefficient (ICC) 0.76; lateral wall strain, ICC 0.66). Conventional markers of CRT response also showed close agreement between both methods (ICC 0.61–0.78). Reproducibility of SLICE was excellent for intra-observer testing (all ICC ≥0.76) and good for interobserver testing (all ICC ≥0.61).ConclusionsThe novel SLICE post-processing technique on standard CMR cine images offers both accurate and robust segmental strain measures compared to the ‘gold standard’ CMR-TAG technique, and has the advantage of being widely available.Key Points• Myocardial strain analysis could potentially improve patient selection for CRT.• Currently a well validated clinical approach to derive segmental strains is lacking.• The novel SLICE technique derives segmental strains from standard CMR cine images.• SLICE-derived strain markers of CRT response showed close agreement with CMR-TAG.• Future studies will focus on the prognostic value of SLICE in CRT candidates.

Development of gait motor control: what happens after a sudden increase in height during adolescence?

BackgroundBasic understanding of motor control and its processes is a topic of well-known high relevance. During adolescence walking is theoretically a well-achieved fundamental skill, having reached a mature manifestation; on the other hand, adolescence is marked by a period of accelerated increases in both height and weight, referred as growth spurt. Thus, this period was chosen as a controlled and natural environment for partially isolating one of the factors influencing motor development (segment growth). The aim of the study was to compare gait performance of growing and not growing male adolescents during walking in single task (ST) and dual task (DT), in order to study which are the modifications that motor control handles when encountering a sudden change in segment length.Methods19 adolescents were selected as growing adolescents (they showed a height increase greater than 3 cm in 3 months). A group of BMI-matched peers were selected as not growing adolescents (they showed a height increase lower than 1 cm in 3 months). Measures of acceleration of the trunk (L5 level) were collected using one tri-axial wireless inertial sensor. The participants were asked to walk at self-selected speed back and forth four times in a 10 m long corridor in ST and DT conditions. The following characteristics of gait performance were evaluated using different indices: variability, smoothness, regularity, complexity and local dynamic stability. An unpaired t-test was performed on the two groups for each method.ResultsDifferent indices followed the hypothesized trend in the two groups, even if differences were not always statistically significant: not growing adolescents showed a lower variability and complexity of gait and a higher smoothness/rhythm. Stability results showed a similarly stable gait pattern (or even higher in DT) in the growing adolescents when compared to their not growing peers.ConclusionsThe findings of the present work suggest that growth spurt affects gait variability, smoothness and regularity but not gait stability. It could be argued that sudden peripheral changes of the body affect the manifestation and the performance of gait, but, on the other hand, gait control is able to handle these modifications, maintaining the stability of the system.

Developing Updatable Crash Prediction Model for Network Screening: Case Study of Czech Two-Lane Rural Road Segments

This paper focuses on application of crash prediction models in network screening. The two main questions were ( a) What variables should be involved in the model? and ( b) What length should the modeled period be? Answers to these questions should provide guidelines for developing an updatable crash prediction model (i.e., a model that is both reliable and simple so that its updating for periodical network screening is not highly demanding). Data on approximately 1,000 km (600 mi) of a two-lane rural-road network from South Moravia, Czech Republic, were used. On the basis of 8 years of annual crash frequencies, together with exposure and geometrical variables, several variants of prediction models were developed. To study the quality of the models, a series of consistency tests was applied relative to comparison of the models themselves as well as to their diagnostic performance. As a result, simple crash prediction models (that included traffic volume, segment length, and curvature change rate) were found sufficient for network screening. If one supposes that length and curvature are unlikely to change often, only traffic volume data need to be periodically updated. Consistency analyses indicate that this period should be 4 years. Under these conditions, models are being applied in the studied region. Further planned activities include extensions to intersections and also to other Czech regions.

Three ways of assessing the forces at the knee and ankle while cycling

The analysis of the biomechanics of the leg while cycling is based on assumptions that make the calculations easier or faster. Most of the models and optimisations based on the dynamic chains introduced by Gonzalez & Hull (Hull & Gonzalez, 1988: J. Biomechanics, 21(10), 839-849) and by Papadopoulos (Papadopoulos, 1987: Biomechanics in Sport, 6, 83-92) with 5 and 4 links respectively share many of these assumptions. For instance, a fixed or no distance from the seat to the centre of rotation of the hip, a constant length of the segments of the leg (thigh, shank and foot) or a constant angular speed of the crank. Previous work (Rios Soltero et al., 2014: Procedia Engineering, in press) has shown that these assumptions may have an influence on the inertial forces of the system. The present study shows that the generalised forces at the knee and ankle would also be different. As it was done before (Rios Soltero et al, 2014), the dynamic chain consisted of three leg segments (thigh, shank and foot), a crank and a grounding element. Also, three motion models were used to calculate the forces at each instant of the pedalling cycle in the sagittal plane at the knee and ankle (Figure 1.a). Here, the analysis was based on the force data at the pedal reported by Boyd et al. (1996, J. Biomechanics, 29(8), 1105-1110), and the motion capture of markers located at the leg and pedal from Rios Soltero et al. (2014). The crank speed was calculated from the pedal marker. To get the orientation angle of each segment, the first two models used a linear regression of their markers. One of them (Inters; intersections method) intersected each line of contiguous segments (thigh with shank and seat-tube, shank with foot and thigh, foot with crank and shank, and crank with seat-tube and foot) to create a dynamic chain at each instant. This allowed a change in length of the segments and the motion of the hip. The second method (Proj; projections method) only uses the orientation of the linear regression for each segment, while their lengths were constant as measured directly from the person. In this way, neither the hip nor the length of the segments were fixed. The third method (Kin; kinematics method) reproduced the assumptions of other studies: a hip fixed at the seat and unvarying length of the leg segments. The length of the leg segments was calculated according to anatomical tables (Winter, 2004: Biomechanics and motor control of human movement (3d ed.). John Wiley & Sons). To calculate the position of the thigh at each instant, an 8th-degree polynomial regression was calculated between the angle of the crank and that of the thigh. In all cases, the centre of mass and mass-moment of inertia of the thigh and shank were calculated using an analytical equation based on the geometry proposed by Durkin Teague et al. (2005: J. Biomechanical Engineering 127, 455-464) and Durkin and Dowling (2006: Annals of Biomedical Engineering 34, pp. 1483-1493). An elliptical frustum was used for the foot. The results of the analysis show a slight difference in the moments at the ankle and the knee for the first half of the cycle (Figure 1.b). The maximum absolute difference in the case of the knee is 15.4 N·m (between Inters and Kin) which is around 13% of the values' range. As for the ankle, it is 7.79 N·m (between Inters and Proj) with a 19% of the values' range. It is clear that there is no qualitative difference between the models. However, further analysis is needed to determine if there is a significant effect.

Investigating the safety effects of road width on urban collector roadways

Collector roads are designed to provide a balance between traffic safety, mobility, and land access; however, the literature on the effects of collector lane width on safety is generally inconsistent. This study determines the safety implications of oversized collectors by analyzing collisions statistics, traffic-survey data, and roadway-inventory data from the City of Edmonton. The paper adopts a cross-sectional evaluation methodology by developing negative binomial (NB) safety performance functions (SPFs) for total collisions. Based on the NB SPFs, it was found that segment length, traffic volume, access-point density and midblock change was statistically significant and positively related to collisions, while the width was negatively related to collisions and statistically significant. The interaction term of volume and lane width was found to be positively related to collisions. This suggests that the collision rate for oversized collector roads is higher than that for standard-sized roads. It was also found that both standard-sized and oversized roads, with some form of midblock roadway width change, were less safe than those with uniform width. Converting oversized roads to standard size was found to improve safety only for the roads with an average daily traffic (ADT) volume higher than 4000. The maximum crash reduction was estimated to be as high as 28.9%. This study is timely given the contradicting evidence that exists in current literature regarding the relationship between safety and oversized collector roads. For the present data set, this paper provides guidelines on when to convert oversized collector roads to standard-sized collector roads, to improve safety.

Intraventricular pressure gradients throughout the cardiac cycle: effects of ischaemia and modulation by afterload

The aim of the present study was to characterize the intraventricular pressure gradients (IVPGs) througout the cardiac cycle, to correlate them with myocardial segmental asynchrony and to evaluate the effects of ischaemia and modulation by afterload. Open-chest anaesthetized rabbits (n = 6) were instrumented with pressure-tip micromanometers placed in the apex and outflow tract of the left ventricular (LV) cavity and with sonomicrometer crystals placed in the apex and base of the LV free wall to measure IVPGs and myocardial segment length changes during basal, afterloaded (aortic cross-clamping) and ischaemic conditions (left anterior descending coronary artery ligation). During early diastole (rapid filling), we recorded an IVPG (4.6 ± 0.7 mmHg) from the cardiac base towards the apex followed by an apex-to-outflow pressure gradient (3.6 ± 0.2 mmHg). During systole, we recorded an IVPG (0.6 ± 0.1 mmHg) from apex to outflow during early rapid ejection, which inverted during late slow ejection. Interestingly, the maximal rate of LV pressure fall occurred earlier and relaxation rate was faster in the base than in the apex. While shortening of basal segments was complete at the end of ejection, apical segments always showed a significant amount of postsystolic shortening. The IVPGs were entirely lost during ischaemia and attenuated by afterload elevations. During ischaemia, systolic shortening of the apical segment decreased, while postsystolic shortening increased. The present study confirms the existence of diastolic and systolic IVPGs in the LV and demonstrates, for the first time, that this normal gradient pattern is related to physiological asynchrony between basal and apical myocardial segments. Moreover, we show that the IVPG, a marker of normal left ventricular function, can be attenuated, lost entirely or even reversed after regional acute ischaemia and afterload elevations.