Changes In Periodontal Tissues Research Articles

Effect of Osteogenic Metabolic Differentiation of Silver Nanoparticles-based Periodontal Ligament Fibroblasts on Orthodontic Tooth Movement.

AgNPs were synthesized using a tannic acid reduction method and characterized accordingly. Fifteen Sprague-Dawley rats were randomly assigned to Normal group, Group A (orthodontic tooth movement after alveolar bone defect repair with a blood clot), and Group B (orthodontic tooth movement after alveolar bone defect repair with AgNPs), with five rats in each group. Morphological changes in periodontal tissues were visualized. hPDLFs were treated with 0 μM (Ctrl), 25 μM (L-AgNPs), 50 μM (M-AgNPs), and 100 μM (H-AgNPs) AgNPs to assess cell proliferation via the MTT assay, calcification via alizarin red staining, and osteogenic differentiation and genes/proteins' expression associated with the I3K/Akt signaling pathway through quantitative polymerase chain reaction and Western blot. AgNP diameter was approximately 20 nm. Relative to the normal group, both Group A and Group B exhibited increased widths of the periodontal ligament (PDL) while displaying a decrease in cell counts within the PDL (P < 0.05). Furthermore, the L-AgNPs, M-AgNPs, and H-AgNPs groups exhibited a notable elevation in the number of calcified nodules in hPDLFs, along with elevated alkaline phosphatase, Runx2, osteocalcin, osterix, type I collagen, phosphorylated phosphoinositide 3-kinase, and phosphorylated protein kinase B versus Ctrl (P < 0.05). AgNPs are beneficial in enhancing the biological functions of the PDL, promoting the repair and regeneration of periodontal tissues, indicating their potential clinical value in orthodontic treatments.

Protective effect of carvacrol hydrogel on the alveolar bone in rats with periodontitis.

This study aimed to investigate the protective effect and mechanism of carvacrol hydrogel on the alveolar bone in rats with periodontitis. A thermosensitive hydrogel supported by carvacrol was prepared using poloxamer and hydroxypropyl methyl cellulose as matrix. SD rats were randomly divided into blank group, periodontitis group, blank hydrogel group, and low-, medium-, and high-dose hydrogel groups. The periodontitis symptoms and the CT structure of the alveolar bone were observed. The changes in liver, spleen, kidney, and periodontal tissues were observed. The related indexes of bone metabolism in serum were detected. The expression of osteoprotegerin (OPG) and nuclear transcription factor-κB (NF-κB) pathway proteins was determined by Western blot. The levels of inflammatory factors were assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Carvacrol hydrogel had good slow release, biocompatibility, and cell adhesion. The periodontitis of rats in the carvacrol hydrogel group was significantly alleviated, the expression of OPG protein in gingival tissue was significantly increased (P<0.01), and the levels of receptor activator of NF-κB ligand (RANKL), receptor activator of NF-κB (RANK), NF-κB protein, and inflammatory factors were significantly decreased (P<0.01). Carvacrol hydrogel can regulate the OPG and NF-κB pathways, reduce alveolar bone absorption, and improve periodontal inflammation.

TANNERELLA FORSYTHIA AS ONE OF THE PREDICTORS OF THE SEVERITY OF CHRONIC GENERALIZED PERIODONTITIS

Chronic generalized periodontitis ranks second in prevalence among diseases of the maxillofacial region in the world. The high importance of the problem of the incidence of chronic generalized periodontitis among modern society is determined by early tooth loss, decreased chewing efficiency, and the formation of chronic foci of infection. It is known that the main factor in the etiology of chronic periodontitis is microbial. One of the representatives of this flora is Tannerella Forsythia, which is a gram-negative, anaerobic bacterium. The aim: to verify T. Forsythia in chronic generalized periodontitis, depending on the severity of the disease. Materials and methods: the total number of patients with chronic generalized periodontitis for scientific research was 126 patients. The patients of all the studied groups were taken from the periodontal pocket, and the control group had the contents of the gingival furrow. All samples were examined by PCR with the detection of results in real time on the DT-96 detecting amplifier of NPO DNA Technology LLC with the Periodontoscreen kit. The results of the study: the value of the correlation coefficient was 0.997 p(0.05), which indicates a direct strong relationship between the occurrence of this microorganism and the severity of the disease. Based on the conducted study, a direct strong relationship (0.948 p(0.05)) was revealed between the concentration of the genomic equivalent of Tannerella Forsythia and the severity of chronic periodontitis. Conclusion: as a result of the study, a direct correlation was found between an increase in the genomic equivalent and an increase in the degree of destructive changes in periodontal tissues. Thus, the results obtained confirm that the concentration of Tannerella Forsythia is a predictor of the severity of chronic periodontitis.

Changes in Periodontal Tissues With Periodontally Accelerated Orthodontics: A Systematic Review and Meta-Analysis.

Periodontally accelerated orthodontic (PAO) therapy has been found to increase hard tissue, helping to decrease orthodontic relapse rates and improve retention capacity. The aim of this study was to synthesize available evidence on clinical and tomographic changes in periodontal tissues when using PAOtechniques. A systematic review with meta-analysis was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and registered in the International Prospective Register of Systematic Reviews (PROSPERO). The search was carried out in PubMed, Embase, Cochrane, Web of Science, Scopus, and Google Scholar. Randomized and non-randomized clinical trials comparing PAO versus conventional orthodontics were included. Quality assessment was performed using the Downs & Black scale, and the risk of bias was assessed using the revised Cochrane risk-of-bias tool. Mean differences and 95% confidence intervals (CIs) were calculated, and the mean difference was divided by a t-test. During the initial search, 465 studies were identified. Five articles studying 130 patients were included, which assessed both clinical and tomographic changes, along with treatment duration. PAO was administered to patients with skeletal class III in three studies, to class II patients in one study, and to individuals with dental crowding in another study. Two studies showed a moderate risk of bias, and the rest showed a low risk. The meta-analysis revealed a vestibular bone thickness increase of 0.32 mm (0.56-008; P = 0.008), a reduction of 3.12 mm (2.15-4.08; P= 0.001) in gingival retraction, and a treatment duration that was 7.07 months (8.79-5.36;P = 0.001) shorter in patients subjected to PAO compared to those undergoing conventional orthodontic treatment. Considering the limitations of the study and acknowledging that definitive conclusions cannot be drawn, the findings suggest that treatment time decreased in patients undergoing PAO, with an increase in vestibular bone thickness and less gingival retraction observed in those undergoing this intervention.

Overexpression of the receptor for resolvin E1 (ERV1) prevents early alveolar bone loss in leptin receptor deficiency-induced diabetes.

This study was designed to test the hypothesis that the leptin receptor (LepR) regulates changes in periodontal tissues and that the overexpression of the receptor for resolvin E1 (ERV1) prevents age- and diabetes-associated alveolar bone loss. LepR-deficient transgenic (TG) mice were cross-bred with those overexpressing ERV1 (TG) to generate double-TG mice. In total, 95 mice were divided into four experimental groups: wild type (WT), TG, LepR deficient (db/db), and double transgenic (db/db TG). The groups were followed from 4weeks up to 16weeks of age. The natural progression of periodontal disease without any additional method of periodontitis induction was assessed by macroscopic and histomorphometric analyses. Osteoclastic activity was measured by tartrate-resistant acid phosphatase (TRAP) staining. At 4weeks, ERV1 overexpression prevented weight gain. From Week 8 onward, there was a significant increase in the weight of db/db mice with or without ERV1 overexpression compared to the WT mice, accompanied by an increase in glucose levels. By 8weeks of age, the percentage of bone loss in the LepR deficiency groups was significantly greater compared to WT mice. ERV1 overexpression in the db/db TG mice prevented early alveolar bone loss; however, it did not impact the development of diabetic bone loss in aging mice after the onset of weight gain and diabetes. The findings suggest that the overexpression of ERV1 prevents LepR-associated alveolar bone loss during the early phases of periodontal disease by delaying weight gain, diabetes onset, and associated inflammation; however, LepR deficiency increases susceptibility to naturally occurring inflammatory alveolar bone loss as the animal ages, associated with excess weight gain, onset of diabetes, and excess inflammation.

Implementation of a predictive strategy in the diagnosis of inflammatory periodontal diseases

Relevance. The diagnosis of periodontal diseases, considering their severity, prevalence, progression, and staging, can be achieved by determining the levels of biomarkers or molecular imaging biomarkers in biofluids such as crevicular or sulcular fluid (GCF or GSF), saliva, and oral fluid. GCF is currently regarded as one of the diagnostically significant biological fluids for assessing the condition of periodontal tissues, not only in clinical diagnostic laboratories but also in dental offices. The implementation of sensitive, highly accurate, non-invasive, and specific methods for rapid GCF diagnosis, based on the qualitative analysis of biomarkers of cytokine imbalance, immunological disorders, changes in non-specific defence factors, and biophysical indicators, will allow for an objective assessment of the condition of periodontal tissues.Purpose. To improve the efficiency of periodontitis prevention using a developed mathematical model for personalized prediction of the course of inflammatory periodontal diseases based on the investigated biomarkers in GCF.Material and methods. The study included 101 patients: Group I consisted of 22 patients diagnosed with K05.10 (gingivitis), Group II included 31 patients diagnosed with K05.31 (mild periodontitis), and Group III comprised 18 patients diagnosed with K05.31 (moderate periodontitis). The comparison group consisted of 30 individuals with clinically healthy periodontium. All subjects underwent clinical and instrumental examination, determination of periodontal indices, GCF collection, and quantitative analysis of immune regulatory mediators (IL-1β, IL-6, TNF-α, IL-8, MCP-1, IL-17, VEGF, IL-1RA).Results. The study of immune regulatory mediators confirmed the significance of increased levels of pro- and anti-inflammatory cytokines/chemokines, as well as the reduction of the anti-inflammatory biomarker IL-1RA in GCF at the early stages of inflammatory changes in periodontal tissues. This is accompanied by the appearance of signs indicating the destruction of the dentogingival junction. Using logistic regression and training a multiclass classifier based on the support vector machine method, a model was developed to predict the risk of dentogingival junction loss in patients, potentially leading to periodontitis.Conclusion. The results of logistic regression modelling and training a multiclass classifier based on the support vector machine method demonstrate that in diagnosing the initial stages of periodontal tissue damage with the loss of the dentogingival junction (DGJ), the most effective approach is the comprehensive use of inflammatory process biomarkers and the development of multi-marker algorithms based on a computer program.

Role of connexin 43 in a rat model of periodontitis-induced renal injury.

This study aims to investigate the role of gap junction mediated by connexin 43 (Cx43) in renal injury induced by periodontitis in rats. Twelve SPF-grade Wistar male rats were divided into a control group and a periodontitis group by using a completely random number table method, with six rats in each group. The control group rats were not treated, while the periodontitis group rats were subjected to wire ligation of the neck of their bilateral maxillary first molars to construct a periodontitis model. After 8 weeks of modeling, the rats were examined for clinical indicators of the periodontium. micro-CT scanning of the maxilla reconstructed its 3D structure and analyzed the absorption of alveolar bone. Histopathological changes in periodontal and renal tissues were detected. MitoSOX red reagent was used to determine reactive oxygen species (ROS) content in renal tissues. A biochemical reagent kit was used to detect serum oxidative stress biomarkers. Real-time fluorescent quantitative-polymerase chain reaction (qRT-PCR) was employed to determine Cx43, nuclear factor kappa-B (NF-κB) , interleukin (IL)-1β, IL-6, BCL2-Associated X (Bax), B-lymphomatoma-2 gene (Bcl-2), and Caspase-3 mRNA were determined. Western blot analysis was used to detect Cx43, NF-κB, IL-1β, Bax, Bcl-2 and Caspase-3 protein. micro-CT 3D reconstruction showed significant bone resorption of the first molar alveolar bone in the periodontitis group rats and decreased height of the alveolar ridge. The distance from the enamel cementum boundary to the top of the alveolar ridge in the periodontitis group was significantly higher than that inthe control group. The histopathological results showed a large number of inflammatory cells that infiltrated the periodontal tissue of the periodontitis group, and the alveolar bone was significantly absorbed. Rats in the periodontitis group also exhibited mild thickening of the glomerular basement membrane, dilation of the Bowman's capsule, and destruction of the brush-like edge of the renal tubules in the renal tissue. The MitoSOX red staining results showed a significant increase in ROS content in the renal tissue of the periodontitis group. The biochemical test results showed that the levels of superoxide dismutase and glutathione in the serum of rats with periodontitis decreased, while that of malondialdehyde increased. The results of qRT-PCR and Western blot showed that the expression levels of Cx43, IL-1β, IL-6, Bax, Caspase-3 mRNA and Cx43, IL-1β, NF-κB, Bax, Caspase-3 proteins in the periodontitis group significantly increased compared with those in the control group, while the expression levels of Bcl-2 mRNA and protein decreased. Periodontitis may activate NF-κB signaling molecules by upregulating the expression of Cx43 in rat kidney tissues, leading to increased levels of inflammation and apoptosis and ultimately inducing kidney injury.

Biologic Width Following Different Crown Lengthening Procedures: A Six-Month Follow-Up Study.

One of the important things to preserve during crown lengthening is the biologic width (BW), recently called supracrestal tissue attachment. A healthy periodontium with adequate BW is very essential for the success of restored teeth. There are various techniques to perform crown lengthening procedures. Most of the studies have focused on assessing the changes in the position of the marginal gingiva and bone as outcome parameters rather than BW. Also, most of the research was done on animal models. The purpose of this study was to assess the periodontal tissue changes at three months and six months following two different surgical crown lengthening procedures. Sixty mandibular first molars among 60 patients that required surgical crown lengthening were enrolled in the study and subjected to two different procedures, gingivectomy (Group I; n=30) and apically positioned flap with ostectomy (Group II; n=30). The following parameters were recorded at baseline, three months, and six months, position of free gingival margin (FGM), probing depth (PD), relative attachment level (RAL), bone level (BL), and BW. These measurements were made at three sites in every patient: treated tooth sites (TT), adjacent tooth's adjacent sites (AD), and adjacent tooth's non-adjacent sites (NAD). The data was then subjected to statistical analysis using SPSS software (Version 20.0). Statistical significance was set to p<0.05. When groups I and II were compared at three and six months, there was no statistical difference in terms of position of FGM, PD, and RAL (p>0.05). When BW was compared between the two groups at three and six months, group II showed better reestablishment of BW at any given time period and was statistically significant (p<0.05). Following surgical crown lengthening, the bone level was shifted apically and allowed for the reestablishment of BW. At six months of follow-up, the apically positioned flap with ostectomy was superior in restoring the BWcompared to gingivectomy.

Radiological, histological and clinical side effects of incisor trimming in rabbits.

Incisor malocclusion in rabbits (Oryctolagus cuniculus) is a common clinical problem seen in general practice. Given that the growth rate is about 2 mm per week, a lack of wear quickly leads to feeding difficulties and soft tissue injuries. Therefore, pathologically elongated incisors must be shortened every three to six weeks. The goal of this study was to assess the potential adverse effects on dental and periodontal tissues associated with the three most commonly used trimming methods: nail cutter, diamond-coated cutting disc and diamond burr. The left mandibular incisor of 28 healthy New Zealand rabbits was subjected to four trimmings with one of the three cutting methods. After the fattening period, the mandibles were collected and both mandibular incisors were investigated on dental radiographs, micro-computed tomography scans and histological sections. Dental and periodontal tissue changes were evaluated. This study allowed a more accurate statement of the potential short-term adverse effects of the three trimming methods. At the clinical level, the nail cutter caused the formation of an irregular occlusal surface with sharp edges. Both engine-drive methods allowed the attainment of a smooth surface but the disc was less accurate. Histological evaluation revealed that the primary modifications, including coronal fractures, periodontal ligament widening and inflammation, reparative osteodentine, paracementosis and biofilm accumulation, were found in the nail cutter group.

Histological changes in the structural components of the gums in lipopolysaccharide periodontitis

Summary. Inflammatory diseases of periodontal tissues are one of the most important problems in dentistry. There is a strong connection between periodontal pathology and inflammatory diseases of the stomach. The aim of the study – to investigate pathomorphological changes in rat periodontal tissues with generalized lipopolysaccharide periodontitis associated with chronic atrophic gastritis. Мaterials and Methods. The experiments were performed on white outbred male rats weighing 160–180 g. 30 animals were used during the work. The tested animals were divided into the following groups: I – intact rats (control); II – animals with a model of generalized lipopolysaccharide periodontitis. For a morphological study of the periodontium, a fragment of the upper jaw was isolated. Histological preparations were studied using an SEOSCAN light microscope. Such classical research methods make it possible to study the structure of tissues, as well as the nature and depth of morphological changes, the sequence of development of destructive and regenerative processes. Results and Discussion. It has been established that with generalized lipopolysaccharide periodontitis, a reorganization of all structural components of the gum tissue occurs. It is manifested by changes in the epithelium of the mucous membrane of the gums, an increase in the stratum corneum, a decrease in the spinous layer, expansion of intercellular spaces, growth of lymphocytic infiltration, swelling of the connective tissue of the mucous membrane, expansion, blood filling of veins and hemocapillaries, cells of the walls of fibrous structures. Conclusions. Concomitant chronic atrophic gastritis significantly aggravates the pathomorphological picture of periodontitis induced in animals by the endotoxin of gram-negative microflora lipopolysaccharide.

The interplay between gingival crevicular fluid microbiome and metabolomic profile in intensively treated people with type 1 diabetes - a combined metagenomic/metabolomic approach cross-sectional study.

This study aimed to assess the gingival crevicular fluid (GCF) microbiome and metabolome of adults with type 1 diabetes (T1D) treated with continuous subcutaneous insulin infusion (CSII). In this cross-sectional study, the GCF of adults with T1D treated with CSII and non-diabetic controls were sampled, and metagenomic/metabolomic analyses were performed. In total, 65 participants with T1D and 45 healthy controls with a mean age of 27.05 ± 5.95 years were investigated. There were 22 cases of mild gingivitis (G) in the T1D group. There were no differences considering the Shannon and Chao indices and β-diversity between people with T1D and G, with T1D without G, and healthy controls. Differential taxa were identified, which were mainly enriched in people with T1D and G. Acetic acid concentration was higher in people with T1D, regardless of the presence of G, than in healthy controls. Propionic acid was higher in people with T1D and G than in healthy controls. Isobutyric and isovaleric acid levels were higher in individuals with T1D and G than in the other two subgroups. The concentration of valeric acid was lower and that of caproic acid was higher in people with T1D (regardless of gingival status) than in healthy controls. The identification of early changes in periodontal tissues by targeting the microbiome and metabolome could potentially enable effective prevention and initial treatment of periodontal disease in people with T1D.

In vivo study of gingival crevicular fluid interleukin 1-beta (IL1-&amp;beta;) and prostaglandin-E2 (PGE-2) levels with pain perception after placement of elastomeric separators with and without low level laser therapy: An in vivo study

Orthodontic tooth movement following application of force features bone remodelling changes in periodontal and dental tissues. These necessary orthodontic tooth movement achieved by numerous orthodontic procedures that evokes pain sensations in patients, such as separator placement, archwire placement and activations. The aim of the study is to compare the levels of interleukin 1-beta and prostaglandin E2 in GCF with pain perception after placement of elastomeric separators with and without low level laser therapy. 12 patients scheduled for orthodontic treatment, were screened test and control. Mesial and distal elastomeric separators flanked the maxillary first molar arch. The experimental side received 20s of Ga Al As, diode laser irradiation at 810 nm, 2 J/cm2, 200 mW power output, while the control tooth did not. GCF was collected from the mesiobuccal and mesiopalatal sides of first molars in the maxillary quadrant before, 1hr, 24 hours, and 48 hours after separator installation from both groups to quantify IL1-b and PGE2. The control and experimental group had IL-1β levels of 18.609 ng/ml (SD = 3.833) and 17.582 ng/ml (SD = 2.425) at the 'Before' time point, with p&amp;#60; 0.001. Significant variations in IL-1β and PGE2 were observed from baseline, with p &amp;#60; 0.001. After 1 hour, IL-1β levels significantly increased to 132.678 ng/ml (SD = 9.628)/ 83.848 ng/ml (SD = 8.833).In the 24-hour interval, IL-1β levels increased dramatically to 185.283 ng/ml (SD = 9.875) and 116.998 ng/ml (SD = 5.680). By 48 hours, IL-1β levels remained high at 157.459 ng/ml (SD = 10.141) and 103.664 ng/ml (SD = 9.662). Low-level laser therapy has been shown to reduce pain perception and decrease inflammatory mediators IL-1β and PGE2 in GCF patients with elastomeric separators. A positive correlation exists between these biomarkers, pain perception, and laser irradiation across all time intervals.

Порівняльна клінічна характеристика застосування цифрових і аналогових відбитків на якість непрямих реставрацій у пацієнтів із дефектами твердих тканин жувальних зубів у різні терміни спостереження

Introduction. Diseases of the hard tissues of the teeth are recognized as one of the most common dental diseases. These are mainly dental caries, congenital defects, fractures, fluorosis or acid erosion. Production of indirect restorations, which require taking impressions of the jaws, is one of the most common methods of restoring the hard tissues of the tooth when they are significantly destroyed. In today’s era of growing interest in digital dentistry, researchers are still undecided as to whether a digital impression provides the same accuracy as a conventional impression for making indirect restorations. Goal. To conduct a comparative characterization of the use of digital and analog impressions on the quality of indirect restorations in patients with defects of the hard tissues of chewing teeth at different follow-up periods. Research material and methods. The research involved 58 patients aged 25-60 years who applied to the Department of Stomatology of the P. L. Shupyk National Healthcare University of Ukraine for the purpose of orthopedic treatment of 79 defects of the hard tissues of the lateral teeth with destruction of 50-60% of their occlusal surface. In the course of orthopedic rehabilitation, patients were made indirect restorations - ceramic inlays using E. MAX Press material (Ivoclar Vivadent, Liechtenstein, Germany). The research group was further divided into 2 subgroups depending on the method of obtaining the impression. Subgroup A included patients who underwent digital scanning of a one-stage two-phase silicone impression, and subgroup B included patients who underwent impression removal using an intraoral digital impression system (TRIOS®, 3shape). The degree of destruction of the occlusal surface of chewing teeth and the choice of indirect restoration was carried out using the index of destruction of the occlusal surface of chewing teeth for I and II class according to Black (IROPZ). The clinical quality of indirect restorations was evaluated 1 week after the installation of the restoration and at long-term periods (12, 24, 36 months) using visual-instrumental control and according to the recommendations of the revised FDI criteria for the evaluation of direct and indirect dental restorations (2023). The presence of inflammatory changes in periodontal tissues was determined using the gingivitis index, the presence of dental plaque was determined using the OHI-S index (Green-Vermillion, 1964), the gingival sulcus bleeding index (SVI) was determined according to the Miihlemann and Son method before indirect restorations, during 1 week, 12 month and 24 month visits after cementation. Conclusion. Dental restorations made using digital impressions showed similar marginal discrepancy as restorations made using traditional impression methods (P&gt;0.05). Lateral masticatory restorations fabricated by intraoral scanning using CAD/ CAM technology have acceptable results in terms of periodontal tissue condition, color, contour, marginal adaptation, and occlusion. Although both direct and indirect digitization have been shown to be equally effective, digital impressions have shown advantages in patient acceptance of the procedure. They take less time for both the doctor and the patient and facilitate the digital workflow. No significant difference was found between conventional and digital displays in the accuracy of the 3D data sets. Key words: defects of hard dental tissues, indirect restorations, digital and traditional impression, inflammatory changes in periodontal tissues, marginal adaptation.

Assessment of the condition of organs and tissues of the oral cavity in people with dentition defects against the background of metabolic syndrome

The aim of the study is comparative analysis of the condition of oral organs and tissues in people with metabolic syndrome (MS) of varying severity before orthopedic dental rehabilitation based on dental implants. 255 patients (151 women and 104 men) aged from 35 to 65 years were examined. 3 groups were formed: 2 study groups and a comparison group. Groups 1 and 2 included individuals with excess body weight and MS. The control group consisted of 88 people without MS. An index assessment of the condition of the periodontium and tissue structures of the alveolar bone (according to cone-beam computed tomography), microcirculation in the gingival mucosa was carried out using laser Doppler flowmetry. The analysis of the periodontal condition indicators showed that in all groups of patients with MS, periodontal pathology occurred, the value of which was significantly higher than in patients of the control group (p <0.05). The analysis of bone tissue according to CBCT data showed that the most favorable conditions (type 1 and type 2 of bone according to Misch) for dental implantation are found in people without MS, respectively 3.5% and 35.1% of cases. The intensity of blood flow (σ) was 21.2% lower in group 1 and 48% in group 2, compared with the control group. Vasomotor activity (Kv) was 13.2% lower in the first group and 35% lower in the second group. A decrease in amplitudes in the area of all rhythms in the LDF gram was found: low-frequency - by 15.6%, high-frequency - by 16.9%, pulse - by 3.6%. Changes occurring in the organs and tissues of the mouth against the background of MS of varying severity lead to a decrease in tissue perfusion with blood and blood flow activity, a local decrease in bone density, and as a result, pathological changes in periodontal tissues. Before performing dental rehabilitation, it is necessary to take into account all the risks of possible complications caused by the general condition of organs and systems of people with MS.

CHANGES IN THE WIDTH OF THE PERIODONTAL GAP OF RATS WITH SUPRAOCCLUSAL RELATIONSHIPS OF INDIVIDUAL TEETH IN THE AGE ASPECT

Introduction. The dentoalveolar apparatus is a complex of complicated interaction of the jaws, masticatory muscles, teeth, and temporomandibular joints. This connection is carried out by the trigeminal nerve system with sensory and motor nuclei closely connected to the cortical and subcortical centers of the brain. Occlusal trauma is a lesion that develops in the periodontium as a result of forces that exceed the periodontal potential for functional adaptation. Occlusal trauma can be classified as primary or secondary. The first one occurs in the pulp and periodontal ligament when the force of the action on the dental structures is greater than usual, under optimal periodontal support conditions. It includes orthodontic movements, "high" restorations, and parafunctional habits such as bruxism. Secondary occlusal trauma occurs when the teeth with previous supporting tissue changes are subjected to the action of normal and excessive forces with supporting tissue changes caused by periodontal disease or previous trauma. The available evidence has demonstrated that bacterial plaque bioactive substances lead to local inflammatory responses in periodontal tissues which contribute to bone tissue resorption through osteoclasts in periodontitis. Excessive functional stress can initiate inflammatory changes in the periodontium and thus, increase destructive bacterial processes. Traumatic occlusion can expand the space of the periodontal ligament and in this way stimulate apical bacterial migration and proliferation. However, few attempts have been made to investigate the molecular mechanisms that trigger bone tissue resorption in traumatic occlusion. The analysis of publications on the studies of morphological changes in periodontal tissues in individual teeth supraocclusion in the age aspect shows the contradictory data. The aim: to determine the morphometric changes of the periodontal gap as a component of the periodontal complex in rats of different age groups in the supraocclusal relationship of individual teeth in the age aspect. Materials and methods of the study. The scientific experiment was conducted on 60 white laboratory rats aged 4 to 22 months and weighing 90 to 330 g. The rats were divided into control (30 animals) and experimental (30 animals) groups. Each group was divided into 3 subgroups (10 animals each) depending on the age of the rats: young, mature and senile. Young animals included rats aged 3–4 months with a weight of 90–100 g; mature animals - aged 6–8 months with a weight of 150–170 g; senile rats – 20–22 months and weighing 200–220 g. The method of modelling the state of supraocclusal relations of individual teeth is described in the previous publication. For histological examination of dental blocks, the following was performed. Fixation in a 10% solution of neutral formalin for two days, dehydration in alcohols of increasing concentration and embedding in paraffin. After preparation, sections were made on a Shandon Finesse 325 rotary microtome with a section thickness of 4-6 µm. The histological sections were fixed on pre-degreased slides and dried. Van Gieson staining was performed. The width of the periodontal gap was determined using the ZEISS ZEN 3.7 program by Carl Zeiss Microscopy. For statistical data processing, the AtteStat v.12.5 software was used to determine the mean value and its standard error (M±m). The probability of a statistically significant difference between the data obtained was assessed using the Student's parametric test (at a significance level of p ≤ 0.05). All samples were tested for normality using the Gupta test. Results of the study. Statistical differences were observed in rats of all groups, as well as between the indicators of all rats of the control and experimental groups (≤0.05). The width of the periodontal gap increased by 34.1% in young rats, by 25.5% in mature rats, and by 21.1% in senile rats. Conclusions The presence of supraocclusal relationships of individual teeth causes an increase in the periodontal gap in rats of all age groups. The greatest expansion of the periodontal gap has occurred in young rats. The width of the periodontal gap in rats decreases with age.

АНАЛОГОВЫЙ И ЦИФРОВОЙ ПРОТОКОЛЫ ОЦЕНКИ ИЗБИРАТЕЛЬНОГО ПРИШЛИФОВЫВАНИЯ ЗУБНЫХ РЯДОВ С ОККЛЮЗИОННЫМИ НАРУШЕНИЯМИ ПЕРЕД ДЕНТАЛЬНОЙ ИМПЛАНТАЦИЕЙ

For successful orthopedic treatment using dental implants, an objective diagnosis of pathological changes in teeth and periodontal tissues is necessary. The identification and optimization of occlusive disorders at various stages of preparation for dental surgery contributes to the normalization of osseointegration processes, expands indications for periodontal diseases. The aim of the study was to assess the normalization of occlusive disorders before dental implantation using analog and digital protocols. The study included 57 patients aged 30 to 65 years and divided into 3 groups: the first group –23 patients with chronic generalized periodontitis of mild severity; the second group–20 patients with nonphysiological location of individual teeth due to partial defects of the dentition; the control group–14 patients. Results. The assessment of occlusive disorders was carried out using occlusography methods and in the articulator "Stratos -300". In the first group–62.6% of supracontacts, in the second group – 49.3%. Occlusive correction of premature contacts was performed. The control was carried out by electronic axiography. Occlusal harmony of occlusal-articulatory relations has been achieved after selective polishing. A comfortable closing of the teeth and smooth movement of the lower jaw have been achieved. The qualitative and quantitative indicators of the axiogram have improved.

EFFECTIVENESS OF THERAPEUTIC AND PROPHYLACTIC MEASURES IN THE MANAGEMENT OF GENERALISED PERIODONTITIS OF THE INITIAL AND FIRST DEGREE IN PATIENTS WITH ATHEROSCLEROSIS ACCORDING TO PARACLINICAL STUDIES

Summary. In recent years, scientists have been paying great attention to the relationship between dental and general somatic health, where, in particular, they express the opinion that periodontitis may play a role as an independent risk factor for the development of vascular atherosclerosis and its clinical manifestations (coronary heart disease, myocardial infarction, stroke).&#x0D; The aim of the study – to evaluate the effectiveness of the proposed therapeutic and prophylactic measures in the management of generalized periodontitis of the initial I degree in patients with atherosclerosis according to paraclinical indices.&#x0D; Materials and Methods. The effectiveness of treatment of generalized periodontitis of the initial I degree was evaluated on the basis of the dynamics of hygienic and periodontal indices in 26 patients with atherosclerosis: 15 patients – the main group and 11 patients – the control group. To study the intensity and prevalence of the inflammatory process in the gums, we used a modified method of determining the papillary-marginal-alveolar index by C. Parma. The combined periodontal index proposed by Russel was used to determine the severity of dystrophic-inflammatory changes in periodontal tissues. The loss of epithelial attachment was determined by the method of G.F. Beloklitskaya. The degree of gingival bleeding was determined using the papillary bleeding index (PBI). To determine the degree and nature of bone resorption of the alveolar ridge, the radiological index (Rtg) according to Fush was used. The hygienic condition of the oral cavity was assessed by the Fedorova-Volodkina index. The statistical calculation of the results was performed using Statistica 8.0 (StatSoft, USA) and the statistical functions package of Microsoft Excel 2021.&#x0D; Results and Discussion. As a result of our studies, it was found that the use of the proposed therapeutic and prophylactic complex for the management of initial I-degree GP contributed to the improvement of the hygienic state of the oral cavity, which was confirmed by a decrease in the index by 47.58 % 6 months after treatment. Improvement in the clinical condition of periodontal tissues in patients of the main group was confirmed by a decrease in the index scores of the indices: PMA, PBI, HEC, p&lt;0,01, PI, p&lt;0,05, against the background of an increase in the Rtg index, p&lt;0,01, relative to the data before treatment. On the basis of the conducted studies, the effectiveness of the use of our proposed treatment and prevention complex, which included a set of individual hygiene measures, local and general pharmacotherapy, adapted for the successful management of initial I degree GP in patients with atherosclerosis, was proved, and was confirmed by the «stabilization» of the pathological process in 80,0% of the treated patients of the main group against 36,36% of the control group, in whom the treatment of initial I degree GP was carried out according to generally accepted methods. The state of periodontal tissues was objectified as «unchanged» in 20,0% of the main group and 36.36% of the treated control group. «Deterioration» of periodontal tissues was diagnosed only in 27,28 % of patients in the control group.&#x0D; Conclusions. Thus, the use of the proposed therapeutic and prophylactic complex for the management of generalized periodontitis of the initial I degree in patients with atherosclerosis contributed to the improvement of the clinical condition of periodontal soft tissues, which was confirmed by the positive dynamics of hygienic and periodontal indices and was characterized by the «stabilization» of the pathological process in 80,00±5,78 % of the treated patients of the main group against 36,36±7,24 % of the control group.

Influence of antiresorptive/antiangiogenic therapy on changes in periodontal and oral tissue structures: a histomorphometrical analysis in rats

ObjectiveThe objective of this study was to investigate the influence of various antiresorptive and antiangiogenic medications on morphological changes in periodontal and oral tissue structures.Materials and methodsFifty-five Wistar rats randomly received dual application (i.e., at baseline and after 12-weeks) one of the following medications: (1) amino-bisphosphonate [zoledronate (Zo)], (2) RANKL inhibitor [denosumab (De)], (3) antiangiogenic [bevacizumab (Be)], (4) Zo + Be, (5) De + Be or (6) no medication [Control (Co)]. Periodontal and oral tissue biopsies were obtained at 17 (n = 21 animals, Phase 1, (De = 3, De + Be = 3, Zo = 5, Be = 3, Zo + Be = 2, Co = 5) and 29 (n = 34 animals, (De = 8, De + Be = 6, Zo = 2, Be = 7, Zo + Be = 4, Co = 7, Phase 2) weeks after the second drug application. The following outcomes were histomorphometrically assessed: periodontal space width in the coronal (PLS-C, mm) and apical sections (PLS- A), number of empty alveolar bone lacunae in the coronal, apical sections and at the apex at respective tooth sites (EL – C, EL- A, EL- Ap), mucosal thickness at edentulous alveolar ridge areas (MT, mm), and, when present, associated areas of inflammatory cell infiltrates (ICI, mm2).ResultsComparable mean PLS-C, PLS-A, ET-A, ET-C, ET-Ap, and MT values were observed in all experimental groups after Phases 1 and 2. The presence of ICI was identified in 3 animals in the Co group (Phase 1: 1, Phase 2: 2), and 17 animals in the test groups (Phase 1: 4; Phase 2: 14). The estimated ICI surface area was significantly higher in the Zo + Be group, followed by the Zo and Be groups compared to that measured in the Co group. The time (i.e., Phases 1 and 2) was not found to be a predictor for the extent of the ICI area. In all groups, the EL-C, EL-A, and EL-Ap values were significantly higher after Phase 2 compared to those assessed after Phase 1. The MT values were significantly reduced in all groups after Phase 2 compared to those measured after Phase 1.ConclusionsThe present evaluation was not able to find any morphological effects of different antiresorptive and antiangiogenic medications on periodontal and oral tissue structures. The presence of inflammatory cell infiltrates was more frequently observed in the animals administered with antiresorptive and antiangiogenic medications as well as combinations thereof.Clinical relevanceAdministration of antiresorptive and antiangiogenic medications may be capable of inducing inflammatory reactions in periodontal tissues.

Age Related Senescence, Apoptosis, and Inflammation Profiles in Periodontal Ligament Cells from Canine Teeth.

The periapical tissues, including periodontal ligament cells (PDLCs) play an important role in repairing the surrounding tissue of the teeth. A decrease in the regenerative potentiality of resident stem cells (PDLCs) has been suggested to be attributed to the decline of pulp regeneration. Therefore, examining the functional changes in periodontal tissue and cells that occur during the aging process is necessary. The changes in the cementum extract (CE) and PDLCs isolated from young and aged dog teeth were evaluated. PDLCs growth rate, senescence markers, p16 and p21, and proinflammatory cytokines, IL-6, IL-1β, and TNF-α, were analyzed by RT-PCR. Bax, an apoptosis marker, Bcl-2, a marker for cell survival, and IL-6 were examined by Western blot analyses to detect their variance expression in the CE. Our results demonstrated that aged PDLCs exhibit a low growth rate and an increased expression of p16; however, no change has been demonstrated in the expression of p21. The chronic inflammatory molecules, IL-6 and TNF-α, were significantly upregulated compared to young PDLCs. Western blot analyses showed decreased expression of Bcl-2 in the CE of the aged tooth (p < 0.001). Taken together, aging influences the functional changes of PDLCs and CE and increases senescence, chronic inflammation, and apoptosis markers. As a result, donor age is a key factor influencing the utilization of PDLCs for tooth regeneration.

Optical Diagnostics in Periodontology

In terms of clinical performance, the conventional methods for diagnosing periodontitis fall short of ideal. By adding optical techniques to ocular inspection and periodontal probing, these procedures can be made better. The minor changes in periodontal tissues that cannot be visually inspected can be identified and measured constantly over a period of time with the help of optical diagnostic techniques. The removal of subgingival microbial biofilms and dental calculus deposits during debridement of teeth and dental implants can be made more efficient by using magnifying loupes, operating microscopes, and cutting-edge methods based on how light interacts with bacterial deposits, such as differential reflectometry and light-induced fluorescence. The absence of ionizing radiation from optical devices, which enables frequent usage on the same patient in clinical practice, is one of the greatest reasons in favor of their use as diagnostic adjuncts. The periodontium’s soft tissues can be examined using three-dimensional scanning, fluorescence spectroscopy, and optical coherence tomography to see how they relate to natural teeth and dental implants. By limiting the under- and overuse of treatment alternatives, an accurate prognosis-determining system would enable a more targeted deployment of finances, enhancing the appropriateness and quality of dental care. The clinical management of people with periodontal disease may be enhanced by a novel approach to periodontal diagnostics.