Changes In Haematological Markers Research Articles

Temperature and water availability induce chronic stress responses in Zebra finch (Taeniopygia guttata).

Animals initiate physiological mechanisms to re-establish homeostasis following environmental stress. To understand how bird physiology responds to abiotic stress, we quantified changes in hematological markers of chronic stress response and body condition of male zebra finches (Taeniopygia guttata) acclimated for 18 weeks to hot and cool temperatures (daytime temperature: 40°C and 23°C) with water available ad libitum or restricted during half of the active phase. Ambient temperature induced greater chronic stress than restricted water availability. While cool compared to hot temperatures induced higher numbers of heterophils and H : L ratios and declined total leucocyte counts, water restriction decreased the number of lymphocytes compared to water ad libitum. Body condition correlated with hematological parameters showing that birds with better condition had greater capacity to face environmental stress. Therefore, prolonged exposure to cool periods may result in chronic stress in zebra finches, especially, if body condition is weakened.

Hematological Trends in Severe Burn Patients: A Comprehensive Study for Prognosis and Clinical Insights.

Severe burn injuries pose diagnostic challenges, contributing to increased fatality rates with delayed diagnoses. This study aims to identify early risk factors and understand their impact on clinical outcomes by examining hematological dynamics in severe burn cases. The focus includes age-related patterns, total body surface area (TBSA) affected by burns, hospital stay duration, and changes in hematological markers during burn injuries. An analytical cross-sectional study at the burn care center involved 135 participants hospitalized between January 2018 and December 2021. Demographic data and hematological markers were recorded, and statistical analysis was performed using IBM SPSS 25.0. Nonsurvivors exhibited a greater mean TBSA, shorter hospital stay, and an enhanced early immune response indicated by white blood cell count on the first day. Hematological markers, including hemoglobin, red cell count (RCC), and platelet count, showed dynamic patterns over the study period. Marginal variations in platelet counts and intriguing patterns in RCC suggested potential consequences like disseminated intravascular coagulation. The study provides crucial insights into hematological responses to severe burn injuries. Early identification of risk factors, particularly age-related patterns and immune responses, informs clinicians about predicting outcomes and guiding therapeutic interventions. Despite limitations, this work underscores the need for further multicenter research to comprehensively understand the complex relationships between burn injuries, hematological responses, and clinical outcomes.

Phytochemical analysis and acute toxicity of Solanum elaeagnifolium extract in Swiss albino mice

IntroductionThe curative effects of Solanum elaeagnifolium have aroused the interest of botanical medicine researchers. However, no research has been carried out into its potential toxicity. The aim of this study was firstly to screen the secondary metabolites contained in the leaves and fruits of S. elaeagnifolium and their mineralogical composition, and secondly to assess the acute oral toxicity of S. elaeagnifolium in Swiss albino mice. MethodsThe amount of minerals in the plant powder was assessed by inductively coupled plasma atomic emission spectrometry, and phytochemical screening was performed. The hydroethanolic extracts of the leaves and fruits of this plant were prepared using the maceration method. Acute oral toxicity was assessed according to the OECD guideline 423. Mice were given leaf extracts (SEFE) and fruit extracts (SEFR) once at doses of 500 mg/kg, 1000 mg/kg and 2000 mg/kg in an acute toxicity study. The animals were then monitored for 14 days. The animals were then monitored for 14 days. To rule out any potential toxicity, the general behavior of the animals, the clinical symptoms of poisoning, body weight, biochemical and hematological markers, and liver, kidney, and spleen histology were examined. ResultsThe two different plant extracts comprised very important mineralogical elements such as Mg, Zn, and k, as well as secondary metabolites such as polyphenols and flavonoids, these chemical elements were shown to have very important biological activities. An oral administration of a hydroethanolic extract of S. elaeagnifolium leaves and fruits at a dose of 2000 mg/kg resulted in toxicity and death in mice. We found no significant difference in the weight of the mice. For doses less than 2000 mg/kg, biochemical markers in the liver, kidneys, and hematology did not differ significantly from those in the control group. Furthermore, there were no changes in hematological markers. Furthermore, the architecture of the liver, spleen, and kidney was normal and histopathological analysis did not reveal significant adverse effects. ConclusionsThese findings indicate that the plant harbors a significant array of secondary metabolites and minerals. S. elaeagnifolium exhibited signs of toxicity and induced histological, hematological, and biochemical alterations at a dose of 2000 mg/kg body weight. These comprehensive studies will help to improve our understanding of S. elaeagnifolium safety and potential utility, laying the groundwork for its safe use in a variety of settings.

“Impact of Hemodialysis Frequency and Duration on Changes in Haematological Markers and Electrolytes in Patients with End-Stage Renal Disease: A Prospective, Multi-Center Study”

“Impact of Hemodialysis Frequency and Duration on Changes in Haematological Markers and Electrolytes in Patients with End-Stage Renal Disease: A Prospective, Multi-Center Study”

“Impact of Hemodialysis Frequency and Duration on Changes in Haematological Markers and Electrolytes in Patients with End-Stage Renal Disease: A Prospective, Multi-Center Study”

“Impact of Hemodialysis Frequency and Duration on Changes in Haematological Markers and Electrolytes in Patients with End-Stage Renal Disease: A Prospective, Multi-Center Study”

Immunoenhancement Effects of the Herbal Formula Hemomine on Cyclophosphamide-Induced Immunosuppression in Mice

Hemomine is an herbal blend comprising Angelicae Gigantis Radix and other herbs known to have immunomodulatory effects. We examined the immunopotentiating effect of this herbal blend on cyclophosphamide (CPA)-induced immunosuppression. Male mice were assigned to one of six groups: the intact control and five CPA treatment groups (one control, one reference (β-glucan), and three with the application of hemomine at different concentrations; 4, 2, or 1 mL/kg; n = 10 per group). Mice were injected with CPA to induce myelosuppression and immunosuppression, after which they received one of the experimental treatments. In immunosuppressed mice, hemomine treatment alleviated the noticeable reductions in body, spleen, and submandibular lymph node weights caused by CPA; caused changes in hematological markers; induced the reduced levels of serum IFN-γ and spleen TNF-α, IL-1β, and IL-10 by CPA; improved natural killer cell activities in the spleen and peritoneal cavity; and also improved lymphoid organ atrophy in a dose-dependent manner. We demonstrate that hemomine, a mixture of six immunomodulatory herbs, is an effective immunomodulatory agent, with the potential to enhance immunity.

Hyperhydration using different hydration agents does not affect the haematological markers of the athlete biological passport in euhydrated volunteers

ABSTRACT Athlete Biological Passport (ABP) is an indirect approach, implemented by WADA, aimed at detecting blood manipulation based on abnormal changes in haematological markers. Cases report the use of hyperhydration as masking method during anti-doping urine sample collection which could potentially mask suspicious fluctuations on ABP profiles. This study investigated the hyperhydration effect on haemoglobin concentration, reticulocyte percentage and OFF-hr score (an algorithm based on haemoglobin concentration and reticulocyte percentage), with and without recombinant human erythropoietin (rHuEPO) administration. A five-week clinical study performed; Baseline and rHuEPO Phase. Water and a sports drink were used as hyperhydration agents. To examine the hyperhydration effect on the normal ABP profile per volunteer, hyperhydration was implemented at 0, 24 and 48 hours during the baseline. During the rHuEPO phase, volunteers received Epoetin beta (3000 IU) with hyperhydration to be implemented at 0, 24 and 48 hours after drug administration. Blood and urine samples were collected and analysed according to WADA guidelines. No significant effect on ABP markers was observed due to hyperhydration at any time during the study. Pre- and post-hyperhydration data were not statistically different compared to individual baseline data. In conclusion, hyperhydration does not affect the ABP haematological markers under the examined conditions.

Preoperative Changes in Hematological Markers and Predictors of Glioma Grade and Survival.

Background: Preoperative hematological markers that indicate nutritional, coagulation, and inflammation statuses have prognostic value for gliomas. This study aimed to investigate hematological markers with regard to tumor grades, isocitrate dehydrogenase mutations (IDH), age, and sex in patients with gliomas.Methods: From 2008 to 2017, patients with a pathological diagnosis of glioma who underwent surgery were retrospectively enrolled in this study. Information from clinical records, including age, sex, preoperative experiment tests (routine blood tests, biochemistry, and coagulation examinations), pathological results, and IDH status, was collected. A univariable survival analysis was performed. Hematological factors such as the neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte-ratio (PLR), and albumin-to-globulin (AGR) were calculated. The prognostic nutrition index (PNI) was calculated as 10 × serum albumin value (g/dl) + 0.005 × peripheral lymphocyte count (per mm3).Results: Our study included 706 patients. The univariate analysis showed that age, IDH-1, and hematological factors were all significantly associated with overall survival (OS) in patients with gliomas. Our results showed that inflammation markers (NLR, PLR, and fibrinogen) were positively associated with age, whereas AGR was negatively associated with age. The PLR was significantly increased, whereas the AGR and PNI were decreased in women with gliomas, as compared with men. We found that inflammation markers increased and nutrition markers decreased with gliomas grade. However, these hematological markers did not significantly differ with IDH status. NLR was the best single hematological marker for distinguishing glioblastoma (GBM) [0.684 (0.645–0.723)], IDH-wt GBM [0.672 (0.631–0.71)] from other gliomas subtypes. Combinations of age with PNI and age with AGR were the best predictors of GBM [0.750 (0.713–0.786)] and IDH-wt GBM [0.759 (0.719–0.798)], respectively.Conclusion: Preoperative hematological marker levels vary among glioma grades and have high predictive values for GBM.

Safety of a dose-escalated pre-workout supplement in recreationally active females.

BackgroundPre-workout supplements (PWS) have increased in popularity among athletic populations for their purported ergogenic benefits. Most PWS contain a “proprietary blend” of several ingredients, such as caffeine, beta-alanine, and nitrate in undisclosed dosages. Currently, little research exists on the safety and potential side effects of chronic consumption of PWS, and even less so involving female populations. Therefore, the purpose of the present study was to examine the safety of consuming a dose-escalated PWS over a 28-day period among active adult females.Methods34 recreationally active, adult females (27.1 ± 5.4 years, 165.2 ± 5.7 cm, 68.2 ± 16.0 kg) participated in this study. Participants were randomly assigned to consume either 1 (G1) or 2 (G2) servings of a PWS daily or remain unsupplemented (CRL) for a period of 28 days. All were instructed to maintain their habitual dietary and exercise routines for the duration of the study. Fasting blood samples, as well as resting blood pressure and heart rate, were taken prior to and following the supplementation period. Samples were analyzed for hematological and clinical chemistry panels, including lipids.ResultsSignificant (p < 0.05) group by time interactions were present for absolute monocytes (CRL −0.10 ± 0.10; G1 + 0.03 ± 0.13; G2 + 0.01 ± 0.12×10E3/uL), MCH (CRL −0.13 ± 0.46; G1 + 0.36 ± 0.52; G2 -0.19 ± 0.39 pg), creatinine (CRL 0.00 ± 0.05; G1 -0.06 ± 0.13; G2 -0.14 ± 0.08 mg/dL), eGFR (CRL −0.69 ± 5.97; G1 + 6.10 ± 15.89; G2 + 14.63 ± 7.11 mL/min/1.73), and total cholesterol (CRL −2.44 ± 13.63; G1 + 14.40 ± 27.32; G2 -10.38 ± 15.39 mg/dL). Each of these variables remained within the accepted physiological range. No other variables had significant interactions.ConclusionThe present study confirms the hypothesis that a PWS containing caffeine, beta-alanine, and nitrate will not cause abnormal changes in hematological markers or resting vital signs among adult females. Although there were statistically significant (p < 0.05) group by time interactions for absolute monocytes, MCH, creatinine, eGFR, and total cholesterol, all of the results remained well within accepted physiological ranges and were not clinically significant. In sum, it appears as though daily supplementation with up to 2 servings of the PWS under investigation, over an interval of 28 days, did not adversely affect markers of clinical safety among active adult females.Electronic supplementary materialThe online version of this article (doi:10.1186/s12970-015-0074-y) contains supplementary material, which is available to authorized users.

Splenectomy Associated Changes in IgM Memory B Cells in an Adult Spleen Registry Cohort

Asplenic patients have a lifelong risk of overwhelming post-splenectomy infection and have been reported to have low numbers of peripheral blood IgM memory B cells. The clinical value of quantitation of memory B cells as an indicator of splenic abnormality or risk of infection has been unclear. To assess changes in B cell sub-populations after splenectomy we studied patients recruited to a spleen registry (n = 591). A subset of 209 adult asplenic or hyposplenic subjects, and normal controls (n = 140) were tested for IgM memory B cells. We also determined a) changes in IgM memory B cells with time after splenectomy using the cross-sectional data from patients on the registry and b) the kinetics of changes in haematological markers associated with splenectomy(n = 45). Total B cells in splenectomy patients did not differ from controls, but memory B cells, IgM memory B cells and switched B cells were significantly (p<0.001) reduced. The reduction was similar for different indications for splenectomy. Changes of asplenia in routine blood films including presence of Howell-Jolly bodies (HJB), occurred early (median 25 days) and splenectomy associated thrombocytosis and lymphocytosis peaked by 50 days. There was a more gradual decrease in IgM memory B cells reaching a stable level within 6 months after splenectomy. IgM memory B cells as proportion of B cells was the best discriminator between splenectomized patients and normal controls and at the optimal cut-off of 4.53, showed a true positive rate of 95% and false positive rate of 20%. In a survey of 152 registry patients stratified by IgM memory B cells around this cut-off there was no association with minor infections and no registry patients experienced OPSI during the study. Despite significant changes after splenectomy, conventional measures of IgM memory cells have limited clinical utility in this population.

Long driving time is associated with haematological markers of increased cardiovascular risk in taxi drivers

Aims: To examine the association between driving time and changes in haematological markers of increased risks for cardiovascular diseases (CVD). Methods: The authors conducted a cross sectional analysis of baseline...