Changes In Blood Indices Research Articles

Metal Dust Exposure Caused Changes in Blood Indices and Serum Proteins

Background: Amongst the industrial hubs in Pakistan, Wazirabad is renowned for its cutlery industry. Cutlery industry generates heaps of multi-metallic dust in whetting units during the processing of stain fewer steel tools. This dust comprises certain potentially toxic and even carcinogenic constituents, thus pose a serious health threat to the workers involved in its processing. Laborers health and safety is something quite non-seriously considered in most of the developing countries, no different is Pakistan. Present exploration was aimed at searching for the differences, in blood profile and quantitative serum protein profile of a group of laborers in cutlery industry that are directly and regularly exposed to multi-metallic dust.
 Materials and Methods: After taking written consent from the participants, blood samples were drawn for hematological analysis and serum analysis. Hematological analysis was performed with hematological analyzer and serum was subjected to SDS gel electrophoresis for protein profiling.
 Results: Statistically significant changes were observed in the number of RBCs, MCV, HCT and RDW, whereas platelet count was decreased in experimental groups when compared to control group. Serum protein profiling using SDS-PAGE revealed the protein fractions ranging from 73 to 287 kDa. Densitometric analysis has shown changes in the serum proteins of the subjects exposed to metal dust. 
 Conclusion: Chronic exposure to the metal dust induce changes in the hematological parameters as well as serum proteins. The industrial workers should ensure the use of industry specific personal safety equipment.
 Key words: Health hazard, Hematology, Metals, Metal dust, Proteins, SDS-PAGE.

Effect of a liquid with a negative redox potential on the indicators of blood and humoral immunity after resection thin gut

An experimental study was conducted to establish changes in blood indices and humoral immu1nity in rats in the postoperative period with the use of catholyte and anolyte. The experiments were carried out on 45 male rats weighing 290–320 g, which were distributed equally into 3 groups: group 1 — intact animals, group 2 and 3 — animals which underwent surgery in the amount of resection of the small intestine (1.5 cm) with anastomosis end to end.After surgery, rats used catholyte as a drink, instead of drinking water. The surgical wound was treated with anolyte. Blood and the wall of the small intestine in the anastomosis zone were used as a biological substrate. The material was studied on days 5 and 15. A study of the composition of blood and the walls of the small intestine was carried out on days 5 and 15 4. An analysis of the materials showed that the use of catholyte (a liquid with negative AFP-leads to positive changes in blood counts, humoral immunity and phagocytic activity, impaired after resection of the small intestine. The use of anolyte antiseptic (liquid with positive AFP) prevents bacterial contamination of the surgical wound. The studied parameters indicate that catholyte positively affects humoral immunity, and anolyte prevents bacterial insemination of the surgical wound.

Bisphenol-A Induced Changes in Blood Indices of Channa punctatus and Alleviation with Vitamin C

Bisphenol-A Induced Changes in Blood Indices of Channa punctatus and Alleviation with Vitamin C

The Effect of HBB: c.-121C>T Variant [–71 (C>T)] on the β-Globin Promoter: Case Series Study

One of the effective strategies in controlling thalassemia is recognition of carriers, followed by prenatal diagnosis (PND) to prevent the occurrence of new cases. There are some rare mutations and variants, for which there are not enough evidences of their effects, and can lead to misdiagnosis and even cause confusion in decision about termination of pregnancy. That is why it is very critical to know the effect of each mutation on the β chain gene. The variant of HBB: c.-121C>T [–71 (C>T)] located in the CAAT box of the promoter region, is a rare mutation. We report seven patients in Hormozagn Province, Iran, who were referred to the PND Center of Hormozgan University of Medical Science (HUMS), Bandar Abbas, Iran during 10 years (2010–2020). Briefly, this mutation causes minor changes in blood indices [mean corpuscular volume (MCV): 75.0 ± 4.0 fL; mean corpuscular hemoglobin (MCH): 25.8 ± 2.5 pg; Hb A2: 3.4 ± 0.5%] showed anemia with a trait milder than minor β-thalassemia (β-thal). Though the existence of α mutations (deletions/point mutations) along with HBB: c.-121C>T can change blood indices due to the changes in α/β ratio. The phenotype of β-thal intermedia (β-TI) was observed in one case, who was a compound heterozygosity for codon 15 (G>A)/–71(C>T) (HBB: c.48G>A/HBB: c.-121C>T. The analysis of transcription level by real-time polymerase chain reaction (real-time PCR) confirmed that this allele induces a mild β+ phenotype due to a decrease in the transcription level.

The adverse effect profile of acitretin in a pediatric dermatology population—Longitudinal cohort study and recommendations for monitoring

The adverse effect profile of acitretin in a pediatric dermatology population—Longitudinal cohort study and recommendations for monitoring

Haematological changes associated with porcine haemoparasitic infections in Ibadan, Oyo State, Nigeria

The study was carried out between January and July 2016. Blood samples were obtained from 153 pigs by venipuncture and jugular severance at slaughter. The blood samples were examined for all known hemoparasites detectable by light microscopic examination. Haematimetric indices, complete blood cell count and leukocyte differentials were determined. The level of parasitaemia and changes in blood indices were subjected to statistical analysis across seasons. Trypanosoma brucei and Eperythrozoon suis were the only hemoparasites detected in the blood of pigs during the period of sampling. The prevalence of haemoparasitic infections in sampled pigs was 5.23%. T. brucei contributed 3.9% while E. suis contributed 1.31% to the prevalence. Anaemia (PCV<32) was a consistent and significant finding in all parasitemic samples. Eperythrozoon suis caused more severe anaemia (20±9.89) when compared with Trypanosoma brucei (27±3.03). The anaemia caused by E. suis was mostly microcytic normochromic while T. brucei mostly caused normocytic normochromic anaemia. Mild leucopenia was observed in eperythrozoonosis while a moderate lymphocytosis was observed in T. brucei infections. It was observed that in spite of intense chemoprophylaxis and other control measures employed, we still have persistent infections with Eperythrozoon sp and Trypanosomes in our pig population. Further studies should be carried out to detect the possibility of drug resistance by some of these circulating hemoparasites in the pig industry. Attempts should also be made to control the vectors of these parasites which are usually abundant during the rainy season and may be responsible for the higher prevalence recorded during this period.Keywords: Porcine, Infection, Haemoparasites, Haematological changes, Nigeria

Эффективность и безопасность разных режимов назначения фиксированной комбинации периндоприла 10 мг и индапамида 2,5 мг у пациентов с артериальной гипертензией

Objective: The most common strategy to improve blood pressure (BP) control is improving compliance with once daily administration of antihypertensive therapy. Aim: To assess the efficacy, tolerability, safety of different regimes of prescription of combination of perindopril 10 mg and indapamide 2.5 mg.Design and methods. We included 31 patients (56±9.3 years, BMI 30.5±5.3 kg/m2, duration of arterial hypertension - 7.1±5.8 years), on two-component therapy (except combination of perindopril and indapamide) with BP>140/90 mmHg. At baseline ambulatory blood pressure monitoring (ABPM), blood tests were performed in 20 men and 10 women, then, patients were randomized into two groups: morning and evening regimes. Previous therapy was canceled and prescribed a combination of perindopril 10 mg and indapamide 2.5 mg. After 8 weeks of treatment, ABPM and blood tests were re-conducted. Results. Baseline systolic blood pressure (SBP) and diastolic blood pressure (DBP) in the whole group - 148.4±10.0/95.6±10.7 mm Hg. After 1 and 2 months of treatment Noliprel-A bi-forte SBP/DBP decreased to 131.4±8.4/86.6±5.7 mm Hg and to 133.4±11.2/84.5±8.8 mm Hg respectively. ( p <0.001 vs baseline). No additional antihypertensive drugs were added. The combination of perindopril 10 mg and indapamide 2.5 mg effectively reduces BP by ABPM data. There was no difference in terms of BP in morning and evening regime groups. Statistically significant changes in blood indices were not detected. Tolerability was assessed by The combination of perindopril 10 mg and indapamide 2.5 mg effectively reduces BP by ABPM data. There was no difference in terms of BP in morning and evening regime groups. Statistically significant changes in blood indices were not detected. Tolerability was assessed by patients as good to very good.Conclusion. The combination of 10 mg perindopril and indapamide 2.5 mg is effective in achieving and maintaining a target BP levels. The dosing regimen does not affect the efficacy and tolerability and does not cause additional adverse effects when administered in the evening.

Blood Cells Morphology of the SnailBellamya Bengalensis

Morphological characters of haemocytes and seasonal changes in blood indices were studied in Bellamya bengalensis. Two types of haemocytes were identified: I (Ia and Ib) and II. Spherical type I haemocytes (ca. 93%) are cells capable of spread-ing on the substratum and forming numerous pseu-dopodia. Subtypes Ia and Ib are most probably two different development stages of the same cell type. Type II haemocytes are oval cells of stable shape, rarely forming pseudopodia. A wide individual var-iation and significant seasonal changes were found, involving the number of haemocytes and haemato-logical indices.

Physiological and perceptual responses to three consecutive official matches in female boxer. A case study

Summary Study aim: The purpose of this study was to assess the physiological cost of three consecutive official boxing fights played during a 3-day tournament and two non-contact specific drills against handheld pads of the same time-profile as the contest, 4 × 2 minutes with 1-minute intervals between them. This assessment was based on the determination of selected hormones and metabolites in the blood sampled directly prior to the contests and throughout short-term post-contest recovery. Material and methods: A female amateur boxer was enrolled on the study during a 3-day Polish Boxing Championship, where one match was played on each day. The timing of capillary blood sampling during each match and the drill was as follows: 10 minutes prior to the effort, and 3 and 30 minutes after its completion. Cortisol (C), testosterone (T), and glucose (G) were determined in the serum, while lactate (LA) was determined in the blood. In addition, prior to each effort, serum creatine kinase (CK) and urea (U) was determined. Directly after each effort, the perception of fatigue (PF) was rated. Results: G, C, and T during official matches were significantly higher than those during non-contact drills. Post-event G, C, T, and LA were higher compared to pre-event values. Conclusions: An official boxing match produced higher stress than a drill of the same time-profile and similar modality. Changes in blood indices corresponded well with the perception of fatigue.

Metabolic and cognitive coefficients in the development of hunger sensations after pure macronutrient ingestion in the morning

The effect of pure carbohydrate, protein and fat ingestion on different aspects of short-term satiety and their relation to metabolic and cognitive performance indices were studied in 15 healthy male students. Subjects were tested in three sessions for short-term changes in blood indices, indirect calorimetry, different aspects of hunger sensations as well as mood and objective cognitive performance using a repeated-measures, counterbalanced cross-over design. Measurements were made after an overnight fast before and hourly during 3 h after macronutrient ingestion. Preloads were isoenergetic (1670 kJ) spoonable creams with similar sensory properties of either pure carbohydrates, protein or fat. Overall ‘desire to eat’ and ‘gastric emptiness’ represented principal components for overall ‘hunger’ ratings, which were larger after fat and carbohydrate compared with protein ingestion. In the first hour, the hunger suppression of carbohydrates was similar to that of protein and related to changes in β-hydroxybutyrate and insulin concentrations accompanied with a preference for carbohydrate-rich food. In the third hour, it was similar to the low satiating power of fat and related to diet-induced thermogenesis together with a preference for protein-rich food. For all macronutrients feelings of ‘energy’ were negatively related to hunger sensations, whereas objective cognitive performance was positively related. Our findings suggest that the subjective satiating effect of carbohydrates seems to change with time in relation to postprandial metabolic changes, presumably mainly dependent on the glycemic response and diet-induced thermogenesis.

Prolonged Clinical Use of a Heme Oxygenase Inhibitor: Hematological Evidence for an Inducible but Reversible Iron-Deficiency State

The heme oxygenase inhibitor tin (Sn4+)-mesoporphyrin, administered to two 17-year-old Crigler-Najjar type I patients during a 400-day study to lower plasma bilirubin levels, also produced changes, beginning approximately 50 days after initiation of treatment, in hematological and iron metabolism indices consistent with the development of iron deficiency anemia. These indices were responsive to iron supplementation and reverted to normal after termination of inhibitor treatment. Tin-mesoporphyrin enhances biliary heme excretion and inhibits intestinal heme oxygenase when administered orally or parenterally; the changes in blood indices could thus reflect, in part, blockade of heme catabolism and therefore of uptake of heme-derived iron, by intestinal epithelium. This action of the inhibitor suggests that such agents may facilitate studies involving aberrant metabolism of heme-derived iron in humans and that they merit further investigation with respect to their potential value in enhancing iron disposal in certain disorders such as those related, for example, to transfusion-induced iron overload states.