Changes In Area Research Articles

Using radiotracers 137Cs and 210Pbex to document climate change in mountain areas through the estimate of soil erosion rates

Abstract. During the last few decades, a general increase in heavy rainfall events has been documented in many areas of the world. These events have caused changes in soil erosion rates and strongly affected the human activities in mountain areas by reducing the national income obtained from cultivated land. In this context, the use of fallout radiotracers can be an important tool for better understanding the consequences of climate change in these areas and proposing effective countermeasures in order to reduce soil loss. In this contribution, plot experiments carried out in southern Italy that involve the use of 137Cs and 210Pbex measurements were performed to estimate soil erosion rates during the last few decades. The overall results indicate an increase in soil erosion rates during the last 15–20 years and suggest the use of this technique to detect climate change in mountain areas.

Study on the Spatial–Temporal Variation of Groundwater Depth and Its Impact on Vegetation Coverage in Ejina Oasis

Ejina, a representative inland river basin situated in the arid region of northwest China, exhibits a delicate ecological environment and its vegetation coverage is intrinsically linked to regional ecological security. Based on MOD13Q1-NDVI data from 2018 to 2023 and groundwater depth monitoring data during the same period, this study analyzed the spatial–temporal variation characteristics of vegetation coverage and its relationship with groundwater depth in Ejina. It is found that the vegetation coverage in Ejina is generally low and mainly distributed along the riverbanks in the form of strips. During the study period, the overall trend of vegetation coverage showed a fluctuating pattern of first increasing and then decreasing, revealing the fragility of the regional ecology. The groundwater depth shows the characteristic of being higher in the east river than the west, and the trend of groundwater depth along the river flow is first increasing and then decreasing. The spatial groundwater depth indicates that the east river is higher than that of the west river, and the groundwater depth along the river flow first increases and then decreases. In terms of inter-annual changes, the groundwater depth experiences a process of first decreasing and then stabilizing. Further analysis indicates that vegetation growth and coverage in Ejina are significantly affected by water conditions, and areas with high Normalized Difference Vegetation Index (NDVI) values are mainly distributed along the riverbanks. In addition, there is a certain degree of correlation between groundwater depth and NDVI. When the depth of groundwater is too deep or too shallow, the positive correlation between NDVI and groundwater depth increases slightly and the negative correlation decreases slightly. The findings of this study are of great significance for understanding and predicting the response of vegetation coverage to groundwater changes in arid areas, and provide a scientific basis for water resources management and ecological protection in Ejina.

Differential functional change in olfactory bulb and olfactory eloquent areas in Parkinson’s disease

Abstract Olfactory dysfunction, or hyposmia, frequently occurs as a prodromal symptom and ongoing sign of Parkinson’s disease. Functional MRI is a powerful tool for studying functional changes in the olfactory brain regions in patients with Parkinson’s disease. However, existing studies show inconsistent results and no study has measured olfactory functional MRI abnormalities in the human olfactory bulb directly. This is mainly due to the well-known susceptibility artifacts in conventional functional MRI images that affect several key olfactory-eloquent brain regions, and especially the olfactory bulb. In this study, olfactory functional MRI was performed using a recently developed functional MRI approach that can minimize susceptibility artifacts and measure robust functional MRI signals in the human olfactory bulb during olfactory stimulation. Experiments were performed on high magnetic field (7T) in 24 early (< 5 years of parkinsonian symptoms) Parkinson’s disease patients and 31 matched healthy controls. Our data showed increased functional MRI signal changes (ΔS/S) in the olfactory bulb in patients with early Parkinson’s disease, which correlated with behavioral olfactory measures. Temporally, functional MRI signals in the olfactory bulb returned to the pre-stimulus state earlier after reaching peak amplitude in patients with early Parkinson’s disease, implicating a faster olfactory habituation effect. The piriform cortex showed reduced numbers of activated voxels in patients with early Parkinson’s disease, which correlated with behavioral olfactory assessment. Several secondary olfactory regions including the orbitofrontal cortex, temporal pole, and amygdala exhibited reduced numbers of activated voxels and increased functional MRI signal changes in patients with early Parkinson’s disease. Our data also showed that functional MRI results are highly dependent on voxel selection in the functional analysis. In summary, we demonstrate differential spatial and temporal characteristics of olfactory functional MRI signals between the primary and secondary olfactory regions in patients with early Parkinson’s disease. These results may assist the development of novel quantitative biomarkers (especially in the early stages of Parkinson’s disease) to track and predict disease progression, as well as potential treatment targets for early intervention.

An aftermath analysis of caving characteristics and movement of overlying strata in fully mechanized longwall gob.

The large-scale collapse of overlying strata in the gob directly affect the safe production of coal mines; they are also the major causes of geological disasters, such as ground cracks, surface subsidence, and ground collapse. In this paper, the movement and caved characteristics of overlying strata during coal seam excavation are studied by conducting a physical model experiment. Results show that overlying strata have different movement and caved laws during the initial, intermediate, and later mining stages. During the initial mining stage, overlying strata do not collapse, and the subsidence is extremely small. During the intermediate mining stage, overlying strata cave along the vertical direction, and caved height gradually increases. Large numbers of cavities, abscission layers, and fractures exist between caved strata. The fractured area gradually increases upward, and the subsidence increases considerably. During the later mining stage, overlying strata cave along the horizontal direction. The abscission layers between the caved strata of the central are compacted. The compacted area is surrounded by a fractured area. The compacted and fractured areas increase along the horizontal direction. The subsidence curves exhibit a horizontal variation. Overlying strata evolve from the self-equilibrium stage to the vertical collapse stage, and finally, the horizontal collapse stage. The fractured area changes from a no fractured area to a fractured area, increases vertically, and finally, increases horizontally. The subsidence curve changes from extremely small to large, and finally, changes horizontally.

Dynamics of Changes in the Surface Area of Water Bodies in Subsidence Basins in Mining Areas

The Silesian Upland in southern Poland is known as a place where subsidence processes induced by mining activities occur in an area of nearly 1500 square kilometres, with many water bodies that formed in subsidence basins. This study concerned the dynamics of changes in the occurrence, boundaries and area of water bodies in subsidence basins (using orthoimagery from 1996 to 2023), as well as the assessment of the factors underlying the morphogenetic and hydrogenetic transformations of these basins. Within the subsidence basins covered by the study, water bodies occupied a total area that changed from 9.22 hectares in 1996 to 48.43 hectares in 2003, with a maximum of 52.30 hectares in 2009. The obtained figures testify to the extremely dynamic changes taking place in subsidence basins, which are unprecedented within such short time intervals in the case of other morphogenetic types of lakes and anthropogenic water bodies (for instance, from 1996 to 2003, the basin of the Brantka water body in Bytom underwent a more than two-fold change in its area, with RA values in the range of 54.4% to 131.9). A reflection of the dynamics of short-term changes in the water bodies in question in the period from 1996 to 2023 is the increase in the water area of the three studied water bodies, which was projected by linear regression to range from 0.09 hectares/year to 0.56 hectares/year. The area change trends, as determined by polynomial regression, suggest a slight decrease in the water table within the last few years, as well as within the next few years, for each of the studied basins.

Earthquake Environmental Effects: The Case of Late Classical-Hellenistic Helike, Gulf of Corinth, Greece

Human habitat is much controlled by the landscape and its ongoing processes overtime. Some of these processes occur instantaneously and are often triggered by seismic events with a major destructive impact on the human-built environment. Helike, on the southwest shore of the Gulf of Corinth, is a characteristic case of an ancient habitation site bearing witness to repetitious natural disasters from the Early Bronze Age to the Late Antiquity. The Late Classical-Hellenistic site, revived in the Helike plain after the 373 BC earthquake, has been systematically investigated thanks to the multidisciplinary research and excavations of the Helike Project in the last 35 years. This work has significantly enriched the historical seismicity of the region and shed light on past human-environment relationships. The study of the architectural remains excavated by the Helike Project, coupled with geological and soil micromorphological analysis on archaeological soils and sediments of the settlement, demonstrates a constant effort of the Helike people to reconcile with the elements of nature. Our results underline the destruction of a flourishing textile dyeworks operated at the settlement, due to a strong earthquake which triggered extensive morphological changes in a broader area. These changes include co-seismic liquefaction and lateral spreading, and post-seismic changes in the gradient of river channels. The former changes attest to an uplift in the headwater area and subsidence in the lowland plain of the ravine flowing near the ancient site.

Initial Desorption Characteristics of Gas in Tectonic Coal Under Vibration and Its Impact on Coal and Gas Outbursts

The rapid desorption of gas in coal is an important cause of gas over-limit and outbursts. In order to explain the causes of coal and gas outbursts induced by vibration, this paper studies the gas desorption experiments of tectonic coal with different particle sizes and different adsorption equilibrium pressures under 0~50 Hz vibration. High-pressure mercury intrusion experiments were used to measure the changes in pore volume and specific surface area of tectonic coal before and after vibration, revealing the control of pore structure changes on the initial desorption capacity of gas. Additionally, from the perspective of energy transformation during coal and gas outbursts, the effect of vibration on the process of coal and gas outbursts in tectonic coal was analyzed. The results showed that tectonic coal has strong initial desorption capacity, desorbing 29.58% to 54.51% of the ultimate desorption volume within 10 min. Vibration with frequencies of 0~50 Hz increased both the gas desorption ratios and desorption volume as the frequency increased. The initial desorption rate also increased with the vibration frequency, and vibration can enhance the initial desorption capacity of tectonic coal and delay the attenuation of desorption rate. Vibration affected the changes in the initial gas desorption rate and desorption rate attenuation coefficient by increasing the pore volume and specific surface area, with the changes in macropores and mesopores primarily affecting the initial desorption rate and 0~10 min desorption ratios, while the changes in micropores and minipores mainly influenced the attenuation rate of the desorption rate. Vibration increased the free gas expansion energy of tectonic coal as the frequency increased. During the incubation and triggering processes of coal and gas outbursts, vibration has been observed to accelerate the fragmentation and destabilisation of the coal body, while simultaneously increasing the gas expansion energy to a point where it reaches the threshold energy necessary for coal transportation, thus inducing and triggering the coal and gas protrusion. The study results elucidate, from an energy perspective, the underlying mechanisms that facilitate the occurrence of coal and gas outbursts, providing theoretical guidance for coal and gas outburst prevention and mine safety production.

Tissue Contraction With Helium-Based Plasma Radiofrequency Technology: A Preliminary Report of Initial Ultrasound Findings

Abstract Background Application of helium-based plasma radiofrequency (RF; Renuvion, Apyx Medical, Clearwater, Florida, USA) in the subcutaneous plane results in tissue coagulation and contraction. While traditionally, impact of thermal devices on skin laxity is assessed by measuring changes in skin surface area, this indirect measure does not permit intraoperative assessment or differentiation of effects from various treatments. Objectives To determine the amount of soft tissue contraction achieved by multiple passes of helium plasma RF energy to the subcutaneous connective tissues following power-assisted liposuction (PAL). Methods In this single-center study, 4 subjects were assessed using real-time ultrasound measurements at baseline and following both PAL and each pass with helium plasma RF. Results Based on changes in the distance from the skin (dermal basement membrane) to Scarpa’s fascia, PAL produced an average reduction of 36.7% (SD 14.6, range 24.7%-58.6%) due to the fat removal. Contraction observed with helium plasma RF was greatest for the first pass, with a mean decrease of 27.0% (SD 19.6, range 4.5%-52.1%) in distance relative to distance measured following liposuction. Subsequent passes resulted in additional incremental contraction relative to prior treatment pass: 17.0% (5.1) for pass 2, 7.7% (17.5) for pass 3, and 7.1% (5.5) for pass 4. Overall, helium plasma RF resulted in an average contraction of 44.0% (SD 17.2, range 27.1%-59.4%) in the skin-to-Scarpa-fascia measurement. Conclusions Helium plasma RF improved tissue contraction following PAL by an average of 44% and data suggest that the greatest increase in tissue contraction is obtained by the first treatment pass.

MAIA and Humphrey Perimetry Differ in Their Estimation of Homonymous Visual Field Defects.

To assess whether the Humphrey Visual Field Analyzer (HFA) and the Macular Integrity Assessment instrument (MAIA) provide equivalent estimates of visual deficit size, severity, and progression in cortically blinded participants. Reliable, monocular 10-2 HFA and MAIA fields were collected at baseline, and after a blind-field training intervention (n = 54) or no intervention (n = 6) in adult participants with occipital strokes. Binocular HFA and MAIA mean sensitivities (MS) were first computed, before creating binocular maps of visual sensitivity for each perimetry system to calculate deficit areas, using a unitary, published, less than 10 dB criterion to define blindness. We contrasted HFA/MAIA MS and deficit area at individual study visits, together with change in these measures between visits. At individual visits, MS and deficit areas were well-correlated, but there were systematic differences between machines, greater for the intact than impaired hemifields, with the MAIA overestimating areas of visual deficit relative to the HFA. Between visits, the two perimeters' assessment of change in MS was correlated, but change in the deficit area was not, despite good test reliability. Reliable HFA and MAIA tests produce well-correlated but systematically offset estimates of visual sensitivity and deficit area in patients with homonymous field defects, which can impact assessment of progression. Cortically blinded patients exhibited comparable changes in sensitivity (spontaneous and training induced) when assessed using Humphrey and fundus-controlled MAIA perimetry. However, the photopic vs. mesopic nature of these tests impacted participant performance and suggests that custom, machine-specific criteria are needed to comparably define blindness on both systems.

Deep Learning for Urban Tree Canopy Coverage Analysis: A Comparison and Case Study

Urban tree canopy (UTC) coverage, or area, is an important metric for monitoring changes in UTC over large areas within a municipality. Several methods have been used to obtain these data, but remote sensing image classification is one of the fastest and most reliable over large areas. However, most studies have tested only one or two classification methods to accomplish this while using costly satellite imagery or LiDAR data. This study seeks to compare three urban tree canopy cover classifiers by testing a deep learning U-Net convolutional neural network (CNN), support vector machine learning classifier (SVM) and a random forests machine learning classifier (RF) on cost-free 2012 aerial imagery over a small southern USA city and midsize, growing southern USA city. The results of the experiment are then used to decide the best classifier and apply it to more recent aerial imagery to determine canopy changes over a 10-year period. The changes are subsequently compared visually and statistically with recent urban heat maps derived from thermal Landsat 9 satellite data to compare the means of temperatures within areas of UTC loss and no change. The U-Net CNN classifier proved to provide the best overall accuracy for both cities (89.8% and 91.4%), while also requiring the most training and classification time. When compared spatially with city heat maps, city periphery regions were most impacted by substantial changes in UTC area as cities grow and the outer regions get warmer. Furthermore, areas of UTC loss had higher temperatures than those areas with no canopy change. The broader impacts of this study reach the urban forestry managers at the local, state/province, and national levels as they seek to provide data-driven decisions for policy makers.

Recent and future variability of the ice-sheet catchment of Sermeq Kujalleq (Jakobshavn Isbræ), Greenland

Abstract Knowledge of ice-sheet catchments is critical for mass-balance assessments, especially glacier-scale input–output budgets. This study explores variations in the catchment of Sermeq Kujalleq, or Jakobshavn Isbrø, Greenland. Six observation-based catchment delineations are evaluated along with a 16-member catchment ensemble calculated from ice-sheet models within the Ice Sheet Model Intercomparison Project for CMIP6 (ISMIP6). The ‘present-day’ ISMIP6 ensemble mean area was found to be $\sim 6.3\%$ larger than the mean of the observed catchments. Ensemble spreads were comparable in size, $\pm 12.3\%$ and $\pm 15.4\%$ , suggesting models are able to delineate the present-day catchment with the same degree of uncertainty as observational methods. The mean catchment area of a 13-member ISMIP6 ensemble shows temporal variation, increasing $\sim [ 2.7,\; \, 5.7,\; \, 9.1] \%$ under three ocean forcing scenarios and a RCP8.5 projection based on one GCM from 2015 to 2100, primarily as the southern catchment boundary migrates southward. This is interpreted as Sermeq Kujalleq exhibiting dynamic piracy, re-directing ice away from adjacent land terminating glaciers. For mass-balance assessments, present-day catchment delineation is more important than capturing the temporal evolution of individual catchments. However, the modeled temporal changes in catchment area are potentially underestimated, as the models exhibit insufficient acceleration of inland ice flow.

Internal septa of the maxillary sinus and their significance in the planning of endodontic interventions, sinus lifting and dental implantation using a collagen matrix scaffold

INTRODUCTION. The study of the structure of the septa in the area of the maxillary sinus floor is important when planning implantation and sinus lifting operations. The height, number and location of the septa can affect the course of the sinus lifting operation. The aim is to study the structure of the maxillary sinus septa, their height, size, localization in order to prevent complications during surgical interventions.MATERIAL AND METHODS. The article analyzes CBCT data from 444 patients for the presence of septa in the area of the lower wall of the maxillary sinus. The subjects were divided into two groups: with the presence of premolars and molars (288 CBCT) and with the absence of at least one chewing tooth (156 CBCT). A study of 48 passportized skulls was conducted, the anterior wall of the maxillary sinus was sawed out, the structure of the sinus floor and septa was studied in detail. The ratio of the volume of compact and spongy bone substance in the premolar and molar areas was also measured.RESULTS. The obtained research data allowed us to obtain new information about the number of septa in the case of a complete dentition and partial tooth loss. Due to changes in the processes of sinus pneumatization, the number of septa increases with partial tooth loss. Their presence increases the risk of perforation of the Schneiderian membrane during sinus lift surgery, including the use of scaffolds based on a collagen matrix. However, when installing an implant in the base of the septum, its stability can be ensured, since the septa are some kind of buttresses of the maxillary bone. The ratio of the volume of spongy and compact bone substance in the premolar and molar area changes with age.CONCLUSION. The study found that the presence of coronal septa can cause complications of sinus lift surgery in the form of perforation of the Schneiderian membrane. At the same time, septa, being buttresses of the maxillary bone, can serve as a basis for installing an implant. With partial loss of teeth, the number of coronal septa increases. With age, the ratio of spongy and compact bone substance changes, towards a decrease in the volume of trabecular and an increase in the volume of compact bone.

Will climate change constrain the altitudinal range of threatened species? Experimental evidence from a biodiversity hotspot.

A fundamental goal in ecology and evolution is to explain the factors that shape species' abundance and range limits. Evaluating the performance of early life-stages across an altitudinal gradient can be valuable for understanding what factors shape range limits and for predicting how plant species may respond to climate change. To experimentally evaluate the presence of local adaptation in a threatened palm (Euterpe edulis) at early life-stages, we reciprocally sowed seeds at two contrasting elevations. In addition, to evaluate the effect of seed predation on E. edulis seed germination and seedling establishment, seed addition experiments were conducted at three different elevations. Our results showed no evidence of local adaptation in the early life-stages for the two E. edulis populations. We observed lower germination and seedling performance of both E. edulis populations at the low-elevation site. The exclusion of seed predation increased seedling establishment across all elevations. Seed predation and dry soil conditions were the main factors that constrained seedling establishment at the upper altitudinal limit and at the lower elevation, respectively. Climate change in the study area will result in warmer and drier environmental conditions. The lack of local adaptation and the lower performance of both E. edulis populations in warm and dry conditions, combined with a higher seed predation at the upper altitudinal limit, might cause an altitudinal range contraction, increasing the vulnerability of this threatened species to climate change.

Combined effects of urbanization and climate variability on water and carbon balances in a rice paddy-dominated basin in southern China

Abstract Urbanization is known to elevate storm runoff, but how it influences carbon cycle and ecosystem productivity through altering the evapotranspiration (ET) process is less clear. We examined the combined effects of urbanization including change in impervious surface area (ISA) and climate variability on the water and carbon balances of the Qinhuai River Basin (QRB) over 2001–2018. QRB represents a typical rice paddy-dominated region that experienced rapid urbanization in southern China. We improved a monthly scale water supply stress index ecosystem service model by integrating local eddy flux measurements and high-resolution remote sensing data. We found a significant downward trend in both ET (−4.6 mm yr−1, p < 0.05) and gross primary productivity (GPP) (−10.4 gC m−2 yr−1, p < 0.05) but a significant upward trend in water yield (Q) (+28.6 mm yr−1, p < 0.05). These ecosystem function changes coincided with a 96% increase in urban areas, 1.9-fold increase in ISA, and a 37% reduction in rice paddy fields. The mean annual watershed GPP decreased from 1048 gC m−2 to 998 gC m−2 while the annual Q increased from 284 mm to 669 mm from 2001 to 2018. Scenario modeling experiments suggested that the negative impacts of loss of rice paddy fields and increase in ISA on ET and GPP overwhelmed the positive impacts of climate warming. The reduction in GPP and increase in Q were largely attributed to the increases in ISA, not necessarily due to changes in land use types (e.g. urban area). The expansion of urban area, increase in ISA and reduction in leaf area index, and increase in precipitation explained the increase in Q. Our research offers insight about the interactions of carbon and water cycles through the critical ET processes under a changing climate and land surface characteristics at a watershed level. Our modeling tool and analysis provides land managers and policy makers information for designing effective ‘Urban Nature-based Solutions’ to mitigate the negative environmental effects of urbanization on carbon and water resources.

Abstract 4123631: Significance of collagen triple helix repeat-containing protein 1 as a right ventricular biomarker in pulmonary hypertension

Background: Right ventricular failure is a major prognostic determinant of pulmonary hypertension and progresses with myocardial fibrosis. Extracellular matrix proteins play a major role in myocardial fibrosis. Right ventricular biomarkers for right ventricular function and fibrosis are poorly examined in pulmonary hypertension. This study investigated the significance of collagen triple helix repeat-containing protein 1 (CTHRC1), one of the extracellular matrix proteins, as a right ventricular biomarker. Methods and Results: A monocrotaline-induced pulmonary hypertension rat model was used in this study. CTHRC1 expression in the right ventricle was higher in rats with monocrotaline-induced pulmonary hypertension than in controls. CTHRC1 was associated with fibrosis in the rat's right ventricle. Next, human circulating CTHRC1 levels were evaluated in controls (n = 20), pulmonary arterial hypertension (PAH, n = 46), and chronic thromboembolic pulmonary hypertension (CTEPH) patients (n = 64). Human circulating CTHRC1 levels were higher in PAH (P = 0.006) and CTEPH patients (P = 0.011) than in controls (Figure). Human circulating CTHRC levels were correlated with tricuspid lateral annular peak systolic velocity (R = -0.213, P = 0.029), right ventricular fractional area change (R = -0.225, P = 0.017), and right ventricular global longitudinal strain evaluated by magnetic resonance imaging (R = 0.429, P = 0.009). In addition, human circulating CTHRC1 levels were decreased after balloon pulmonary angioplasty (P < 0.001) in CTEPH patients. Conclusions: CTHRC1 could be a novel biomarker associated with right ventricular failure and fibrosis in pulmonary hypertension patients.

Abstract 4118444: Right Ventricular Contractile Reserve and Right Ventricular-Pulmonary Arterial Coupling Are Impaired in Long-Term Survivors of Childhood Cancers

Background: Childhood cancer survivors are at risk of right ventricular (RV) dysfunction in relation to cardiac toxicity due to chemotherapy and radiation therapy. This study tested the hypothesis that RV contractile reserve and RV-pulmonary arterial (PA) coupling are altered in long-term survivors of childhood cancers. Methods: Thirty survivors (60% men) aged 24.3 ± 5.2 years at 15.3 ± 6.3 years after completion of chemotherapy and thirty healthy control subjects (47% men) were studied. Resting and submaximal supine bicycle stress echocardiography was performed for assessment of RV fractional area change (FAC), tricuspid annular plane systolic excursion (TAPSE), left ventricular (LV) and RV longitudinal strain, mitral and tricuspid annular velocities and myocardial acceleration during isovolumic contraction (IVA). The slope of the RV force-frequency relationship was derived from the change in IVA with the change in heart rate during exercise (△IVA/△heart rate), while RV-PA coupling was determined by the ratio between TAPSE and pulmonary arterial acceleration time indexed to RV ejection time (PAATi). Results: At rest, tricuspid annular systolic velocity and RV systolic strain were significantly lower in survivors ( P < 0.05 for both), while RV FAC, TAPSE and IVA were similar between the two groups ( P > 0.05 for all). During submaximal exercise testing, all RV systolic functional indices were significantly lower in survivors than controls ( P < 0.05 for all). The slope of the RV force-frequency relationship was significantly flatter in survivors compared to controls (0.038 ± 0.002 vs 0.059 ± 0.003 m/sec 2 beats/min, P < 0.001). For RV-PA coupling, TAPSE/PAATi was similar between survivors and controls at baseline but became significantly lower in survivors during submaximal exercise ( P = 0.002). Conclusions: The RV contractile reserve and RV-PA coupling are impaired during exercise in long-term survivors of childhood cancers. Future studies aimed at determining the clinical and prognostic significance of these impairments are warranted.

Abstract 4143242: Right Ventricular Remodeling During the First Decade After Tetralogy of Fallot Repair: An Echocardiographic Study

Background: After TOF repair (TOFr), pulmonary regurgitation resulting in right ventricular (RV) dilatation and dysfunction is common. We have previously demonstrated that RV function (RVF) improves in the first two years after TOFr, however RV changes in the first decade after TOFr are less known. We investigated RVF and RV size (termed remodeling) using echocardiography during the first decade after TOFr. Methods: Retrospective cohort study of patients who underwent TOFr and were followed at our institution from 3/2012 to 12/2023. Those with at least two echocardiograms separated by one year were included. Two patients that received a pulmonary valve replacement were excluded. After confirming intra- and inter-reader reproducibility, RV remodeling was assessed offline by global longitudinal strain (GLS), free wall strain (FWS), fractional area change (FAC), end systolic area (ESA/BSA) and end diastolic area (EDA/BSA). RVF changes over time were assessed with multivariable linear mixed effects models adjusting for perioperative covariates. Two years after TOFr (Y2) was used as reference given RVF peaks at this point following surgery. Results: Out of 758 echocardiograms from 116 patients, 688 were suitable for analysis. Median age at TOFr was 3.5 months (IQR:1.92, 4.89). Compared to Y2, we identified the largest decline in RVF at Year 3, with no worsening from year to year until Y10. There were no associations of perioperative covariates with RVF except for ventriculotomy (FAC) and residual pulmonary stenosis (FWS). There was no progression in RV remodeling over the first decade after TOFr. (Graph, table). Conclusion: After the initial 2-year improvement following TOFr, RVF worsens but remains stable throughout the first decade with stable RV size accounting for BSA. Our findings augment the knowledge of RV remodeling during childhood and could help guide outpatient follow up, counseling, frequency of echocardiograms, and timing of the first cardiac MR.

TMOD-15. TOWARD BRAIN CANCER ORGANOID-INFORMED PRECISION MEDICINE FOR GLIOBLASTOMA

Abstract BACKGROUND Improved preclinical models for glioblastoma are urgently needed to enhance the translation of research to clinical applications. Patient-Derived Tumor Organoids (PDTOs) offer a promising model that can be generated quickly. Here we explored their feasibility. METHODS Primary (n=16) and recurrent (n=2) PDTOs were established using the method described in Jacob et al. Cell. 2020 by dissecting tumours into small fragments, preserving their cytoarchitecture. Histological features of the parent tumour were compared to the matched PDTOs by a neuropathologist. To assess the molecular fidelity, comprehensive whole genome sequencing compared matched tumor tissue, PDTOs, and corresponding normal blood samples (n=11), along with RNA sequencing of matched tumor tissue and PDTOs (n=10). We assessed PDTO responses to regorafenib (n=18) and conventional TMZ (n=9), lomustine (n=9) and 10 Gy of ionizing radiation (IR) (n=6) using the automated imaging system IncuCyte S3® to evaluate whole spheroid brightfield and invasion areas following drug/IR treatment and assess viability. RESULTS We achieved a 100% establishment rate of primary GBMs using same-day procedures and an 80% rate if held under 3 days. Histological features were retained in the PDTO. Molecular overlap was greater than 90%, along with a high concordance in gene expression. PDTOs exhibited notable resistance to temozolomide and lomustine, however, showed a very modest response to IR and a moderate response to regorafenib. IncuCyte S3® analysis revealed discernible changes in whole spheroid brightfield and invasion area for regorafenib-treated PDTOs. Clinically relevant concentrations of regorafenib reduced GBM cell proliferation (Ki67 staining). Beyond this concentration, regorafenib exhibited efficacy in eliminating GBM cells. CONCLUSION PDTOs recapitulate the molecular and histological features of the original tumor and may enable the prediction of individual patient responses to therapies and guide more effective treatment selection. We are now applying our established methodology to assess a new set of promising drug candidates.

Thermal Footprint of the Urbanization Process: Analyzing the Heat Effects of the Urbanization Index (UI) on the Local Climate Zone (LCZ) and Land Surface Temperature (LST) over Two Decades in Seville

Urbanization is a multifaceted process characterized by changes in urban areas through various means, such as sprawl, ribbon development, or infill and compact growth. This phenomenon changes the pattern of the local climate zone (LCZ) and significantly affects the climate, vegetation dynamics, energy consumption, water resources, and public health. This study aims to discern the impacts of changes in urban growth on the LCZ and land surface temperature (LST) over a two-decade period. A comprehensive methodology that integrates statistical analysis, data visualization, machine learning, and advanced techniques, such as remote sensing technology and geospatial analysis systems, is employed. ENVI, GEE, and GIS tools are utilized to collect, process, and monitor satellite data and imagery of temporal and spatial variations in intensive or diffuse urbanization processes from 2003 to 2023 to analyze and simulate land use and land cover (LULC) changes, urbanization index (UI), LCZ patterns, and LST changes over the years and to make overlapping maps of changes to recognize the relation between LULC, LCZ, and LST. This study focuses on Seville’s urban area, which has experienced rapid urbanization and a significant increase in average temperature during the last few decades. The findings of this study will provide actionable recommendations into the interplay between urban growth and climate and highlight the pivotal role of urban growth in shaping resilience and vulnerable areas based on microclimate changes. Urban planners can leverage these insights to predict alternatives for the future development of urban areas and define practical climate mitigation strategies.

Comparison of Microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis Lipid Extracts Effects on UVA-Induced Changes in Human Skin Fibroblasts Proteome

Lipid extracts from the microalgae Nannochloropsis oceanica and Chlorococcum amblystomatis have great potential to prevent ultraviolet A (UVA)-induced metabolic disorders. Therefore, the aim of this study has been to analyze their cytoprotective effect, focused on maintaining intracellular redox balance and inflammation in UVA-irradiated skin fibroblasts, at the proteome level. The above lipid extracts reversed the suppression of the antioxidant response caused by UVA radiation, which was more visible in the case of C. amblystomatis. Modulations of interactions between heme oxygenase-1 and matrix metalloproteinase 1/Parkinson’s disease protein 7/transcript1-α/β, as well as thioredoxin and migration inhibitory factor/Parkinson’s disease protein 7/calnexin/ATPase p97, created key molecular signaling underlying their cytoprotective actions. Moreover, they reduced pro-inflammatory processes in the control group but they also showed the potential to regulate the cellular inflammatory response by changing inflammasome signaling associated with the changes in the caspase-1 interaction area, including heat shock proteins HSP90, HSPA8, and vimentin. Therefore, lipid extracts from N. oceanica and C. amblystomatis protect skin fibroblast metabolism from UVA-induced damage by restoring the redox balance and regulating inflammatory signaling pathways. Thus, those extracts have proven to have great potential to be used in cosmetic or cosmeceutical products to protect the skin against the effects of solar radiation. However, the possibility of their use requires the evaluation of their effects at the skin level in in vivo and clinical studies.