Changes In Energy Partitioning Research Articles

Divergent response of energy exchange to heatwaves from flux tower observations among various vegetation types

Abstract The increasing frequency of European heatwaves and the associated impacts on ecosystems have raised widespread concern during the last two decades. The partitioning of surface energy between latent and sensible heat fluxes plays a pivotal role in regulating heat and water exchange between the land surface and the atmosphere. However, the responses of surface energy partitioning during heatwave events and the contributions of changes in energy partitioning to heatwave development have been underexplored. Here, we investigated the responses of surface energy exchange to temperature extremes during four devastating European heatwaves (2003, 2010, 2018, and 2022) based on long‒term observations from 31 flux towers. Our results demonstrated that the divergent responses of surface energy exchange to heatwaves were modulated by vegetation type and background climate in Europe. Forests maintained similar latent heat fluxes as the climatological mean but largely increased sensible heat under heat‒stressed conditions. While grasslands and croplands tended to increase sensible heat by suppressing latent heat during heatwaves, especially under water‒stressed conditions. Furthermore, the changes in surface energy partitioning strengthened positive land‒atmosphere feedbacks during the heatwave period, leading to unprecedented temperature extremes. This study highlights the importance of surface energy partitioning in land‒atmosphere interactions and heatwave developments.

Evolution of ecosystem-scale surface energy fluxes of a newly constructed boreal upland-fen watershed

Obligatory mine reclamation efforts in the Athabasca oil sands region require leased land to be returned to pre-disturbance function. This entails the re-establishment of key ecosystem services such as carbon sequestration and water and nutrient cycling. Surface-atmosphere exchanges of energy, heat and moisture are critical to ecohydrological processes and feedbacks that govern ecosystem functionality. Eddy covariance and remote sensing measurements were used to examine the temporal changes in energy partitioning of a reclaimed fen-upland watershed during the initial seven years post-construction (2013–2019). Surface conditions evolved from bare ground to robustly vegetated plant communities, altering albedo, surface roughness and plant-mediated shading. Initially in the fen, due to near-surface water table, high soil moisture content and the low albedo (0.09) of bare, wet peat, energy was predominantly partitioned to latent heat (QE) and exhibited a high degree of decoupled surface-atmosphere conditions (surface-atmosphere decoupling factor (Ω) of 0.49). As the fen plant community developed and stabilized, QE remained the dominant energy flux, but a larger proportion of available energy was partitioned to sensible heat flux (QH). Plant establishment altered the biophysical surface through sheltering and shading affecting ground heat flux and supressing surface evaporative losses. Moreover, plant-mediated water loss through stomatal controls resulted in an increase in surface-atmosphere coupling (Ω = 0.32), particularly during drier periods. In the drier upland, plant emergence and establishment was slower than in the fen. Initially, bare ground in the upland resulted in higher albedo (0.20–0.25), and QE and QH were similar. Once understory and tree species matured, QE increased with seasonal trends that mirrored plant phenology. Unlike the fen, the upland continually exhibited a higher degree of surface-atmosphere coupling (Ω ranging between 0.23 and 0.3). By year seven (2019) energy fluxes and intra- and inter-seasonal trends at the constructed watershed were comparable to natural and post-disturbance boreal landscapes indicating the system is evolving towards a functional, reclaimed ecosystem.

Resting metabolic rate, abdominal fat pad and liver metabolic gene expression in female rats provided a snacking diet from weaning to adulthood

Our female rat model with continuous, ad libitum access to snacks and chow from weaning to adulthood closely mimics human feeding behavior from childhood onwards. It causes weight gain, enlarged abdominal fat pads, reduced insulin sensitivity and leptin resistance without an increase in total caloric intake. Our current study investigated if this change in energy partitioning is due to a decrease in resting metabolic rate (RMR). In addition, we determined if carbohydrate and lipid metabolism changes in abdominal fat pads and liver. RMR, using indirect calorimetry, was determined in control and snacking rats every two weeks from Days 28-29 to Days 76-77. RMR decreased with age in both groups, but there was no difference between snacking and control rats at any age. At termination, abdominal fat pads (parametrial, retroperitoneal and mesenteric) and liver samples were collected for determination of gene expression for 21 genes involved in carbohydrate and lipid metabolism using RT-qPCR. Analysis of gene expression data showed a striking difference between metabolic profiles of control and snacking rats in abdominal fat pads and liver, with a distinct segregation of genes for both lipid and carbohydrate metabolism that correlated with an increase in body weight and fat pad weights. Genes involved in lipogenesis were upregulated in abdominal fat pads, while genes involved in adipogenesis, and lipid recycling were upregulated in the liver. In conclusion, snacking in addition to chow from weaning in female rats causes a repartitioning of energy that is not due to depressed RMR in snacking rats. Rather, snacking from weaning causes a shift in gene expression resulting in energy partitioning toward enhanced abdominal fat pad lipogenesis, and adipogenesis and lipid recycling in liver.

Association of protein-energy partitioning with body weight and body composition changes in adolescents with severe obesity.

Body composition and protein-energy partitioning changes are important factors of body weight regulation, but have not been studied in the context of clinical obesity treatment setting. Thus, the aim of the present study was to investigate the pattern of body weight loss, body composition, and energy partitioning changes during a 9-month multidisciplinary weight loss program and 4-month follow up and to test the associations among these changes in adolescents with severe obesity. Twenty-five adolescents (14.1 ± 1.5 years old; 13 girls) with severe obesity joined a pediatric obesity center for a 9-month inpatient multidisciplinary weight loss program. All participants performed body composition assessment (i.e. fat mass-FM, and fat-free mass-FFM) and completed a 36-h session in indirect calorimetric chamber before the start (T0), at the end of the intervention (T1) and 4 months follow-up to the intervention (T2). The protein-energy partitioning (P ratio) was calculated as urinary nitrogen loss/total energy expenditure over 24 hours. 9-month individualized multidisciplinary weight loss program consisting of lifestyle education, psychological support, physical activity, and dietary intervention. Initial P ratio was positively associated with changes in body weight from T0 to T1 (p = 0.038). The changes in FFM/FM were negatively associated with body weight changes in boys (p = 0.006) from T0 to T1 and in girls (p < 0.001) from T1 to T2. Urinary nitrogen excretion (p < 0.001) and total energy expenditure (p < 0.001) significantly decreased during the weight loss program while the P ratio did not significantly change. The present results suggest that baseline and changes in energy partitioning may be associated with changes in body weight in adolescents with severe obesity. In addition, sexual dimorphism in these patterns of change suggest the need for specific dietary and physical activity strategies in boys and girls to optimize body weight loss and to prevent or slow weight regain.

Assessment of an Automated Calibration of the SEBAL Algorithm to Estimate Dry-Season Surface-Energy Partitioning in a Forest–Savanna Transition in Brazil

Evapotranspiration ( E T ) provides a strong connection between surface energy and hydrological cycles. Advancements in remote sensing techniques have increased our understanding of energy and terrestrial water balances as well as the interaction between surface and atmosphere over large areas. In this study, we computed surface energy fluxes using the Surface Energy Balance Algorithm for Land (SEBAL) algorithm and a simplified adaptation of the CIMEC (Calibration using Inverse Modeling at Extreme Conditions) process for automated endmember selection. Our main purpose was to assess and compare the accuracy of the automated calibration of the SEBAL algorithm using two different sources of meteorological input data (ground measurements from an eddy covariance flux tower and reanalysis data from Modern-Era Reanalysis for Research and Applications version 2 (MERRA-2)) to estimate the dry season partitioning of surface energy and water fluxes in a transitional area between tropical rainforest and savanna. The area is located in Brazil and is subject to deforestation and cropland expansion. The SEBAL estimates were validated using eddy covariance measurements (2004 to 2006) from the Large-Scale Biosphere-Atmosphere Experiment in the Amazon (LBA) at the Bananal Javaés (JAV) site. Results indicated a high accuracy for daily ET, using both ground measurements and MERRA-2 reanalysis, suggesting a low sensitivity to meteorological inputs. For daily ET estimates, we found a root mean square error (RMSE) of 0.35 mm day−1 for both observed and reanalysis meteorology using accurate quantiles for endmembers selection, yielding an error lower than 9% (RMSE compared to the average daily ET). Overall, the ET rates in forest areas were 4.2 mm day−1, while in grassland/pasture and agricultural areas we found average rates between 2.0 and 3.2 mm day−1, with significant changes in energy partitioning according to land cover. Thus, results are promising for the use of reanalysis data to estimate regional scale patterns of sensible heat (H) and latent heat (LE) fluxes, especially in areas subject to deforestation.

Impact of Soil Moisture on Winter 2-m Temperature Forecasts in Northern China

AbstractIn this study, the causes of the underestimated diurnal 2-m temperature range and the overestimated 2-m specific humidity in the winter of northern China in the Rapid-Refresh Multiscale Analysis and Prediction System–Short Term (RMAPS-ST) are investigated. Three simulations based on RMAPS-ST are conducted from 1 November 2016 to 28 February 2017. Further analyses show that the partitioning of surface upward sensible heat fluxes and downward ground heat fluxes might be the main contributing factor to the 2-m temperature forecast bias. In this study, two simulations are conducted to examine the effect of soil moisture initialization and soil hydraulic property on the 2-m temperature and 2-m specific humidity forecasts. First, the High-Resolution Land Data Assimilation System (HRLDAS) is used to provide an alternative soil moisture initialization. The results show that the drier soil moisture could lead to noticeable change in energy partitioning at the land surface, which in turn results in improved prediction of the diurnal 2-m temperature range, although it also enlarges the 2-m specific humidity bias in some parts of the domain. Second, a soil texture dataset developed by Beijing Normal University and the revised hydraulic parameters are applied to provide a more detailed description of soil properties, which could further improve the 2-m specific humidity bias. In summary, the combination of using optimized soil moisture initialization, an updated soil map, and revised soil hydraulic parameters can help improve the 2-m temperature and 2-m specific humidity prediction in RMAPS-ST.

Liver Glutamate Dehydrogenase Controls Whole-Body Energy Partitioning Through Amino Acid-Derived Gluconeogenesis and Ammonia Homeostasis.

Ammonia detoxification and gluconeogenesis are major hepatic functions mutually connected through amino acid metabolism. The liver is rich in glutamate dehydrogenase (GDH) that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and ammonia, thus bridging amino acid-to-glucose pathways. Here we generated inducible liver-specific GDH-knockout mice (HepGlud1-/- ) to explore the role of hepatic GDH on metabolic homeostasis. Investigation of nitrogen metabolism revealed altered ammonia homeostasis in HepGlud1-/- mice characterized by increased circulating ammonia associated with reduced detoxification process into urea. The abrogation of hepatic GDH also modified energy homeostasis. In the fasting state, HepGlud1-/- mice could barely produce glucose in response to alanine due to impaired liver gluconeogenesis. Compared with control mice, lipid consumption in HepGlud1-/- mice was favored over carbohydrates as a compensatory energy fuel. The changes in energy partitioning induced by the lack of liver GDH modified the circadian rhythm of food intake. Overall, this study demonstrates the central role of hepatic GDH as a major regulator for the maintenance of ammonia and whole-body energy homeostasis.

Effects of intramuscular injections of folic acid, vitamin B12, or both, on lactational performance and energy status of multiparous dairy cows

The purpose of this experiment was to gain understanding on changes in energy partitioning when folic acid and vitamin B12 supplements, alone or combined, were given by weekly intramuscular injections from 3 wk before the expected calving date until 7 wk postpartum. Twenty-four multiparous cows were assigned to 6 blocks of 4 cows each according to previous 305-d lactation yield to either 0 or 320 mg of folic acid and 0 or 10 mg of vitamin B12 in a 2 × 2 factorial arrangement. Plasma concentration of folates was increased by folic acid supplement, and this increase was greater with the combined supplement. Vitamin B12 supplement increased plasma concentration of vitamin B12. Even though postpartum energy balance was similar among treatments, postpartum body condition score was higher for cows receiving folic acid supplement compared with cows that did not. Milk yield of cows receiving folic acid supplement reached a plateau earlier than for cows that did not. Fat and protein, as well as total solid concentrations and yields, were unaffected by treatments. Postpartum plasma concentrations of glucose and insulin were higher and postpartum plasma concentration of nonesterified fatty acids was lower for cows that received weekly folic acid supplement compared with cows that did not. Plasma concentration of methylmalonic acid was low and unaffected by treatments, suggesting that vitamin B12 supply was not limiting, even for unsupplemented cows. Postpartum plasma concentrations of Cys, His, Phe, and Tyr were increased, whereas plasma concentration of Gly was decreased, by folic acid supplement. In the present study, supplementary folic acid altered energy partitioning in early lactation as suggested by similar milk total solid yield and postpartum energy balance, lower plasma nonesterified fatty acid concentration and body condition score losses, and higher plasma glucose and insulin concentrations for cows receiving folic acid supplement compared with cows that did not.

Energy partitioning in herbage-fed dairy cows offered supplementary grain during an extended lactation

An experiment was conducted to quantify the changes in energy partitioning resulting from grain supplementation in herbage-fed dairy cows at 4 stages during a 670-d lactation. The experiment used 16 lactating Holstein-Friesian cows, with a control and a grain treatment being randomly allocated to 8 cows each. During 4 measurement periods (each of 4d in a metabolism stall and 3d in an indirect calorimeter) beginning at approximately 110, 270, 450, and 560d in milk (DIM), the energy balance of each cow was measured. Cows in both groups were individually offered freshly cut ryegrass pasture (Lolium hybridum L.) in periods 1 and 3 and ryegrass pasture silage and alfalfa (Medicago sativa L.) hay in periods 2 and 4. In all periods, cows in the grain group were offered an additional 4.4 to 5.0kg of dry matter of cereal grain/cow per day. Adding grain to the diet increased yields of fat and protein and tended to increase yields of milk and lactose, but did not affect milk composition. Gross energy intake (GEI) declined as lactation progressed. Adding grain to the diet decreased the percentage of GEI in feces and urine, but the extent of these reductions did not change as lactation progressed. Adding grain to the diet similarly reduced the percentage of GEI lost to heat, but again the extent of the reduction remained similar as lactation progressed. The magnitude of the increase in milk energy resulting from grain supplementation did not change with advancing lactation, but tissue energy retention was greater in the first 300 DIM compared with after 300 DIM. For herbage-based diets, CH4 emissions ranged from 6.2 to 7.6% of GEI, which corresponds to 24.0 to 25.8g of CH4/kg of dry matter intake. For diets supplemented with cereal grains, CH4 emissions ranged from 6.3 to 7.3% of GEI, which corresponds to 21.6 to 25.2g of CH4/kg of dry matter intake. It was concluded that, for cows producing <24kg of milk/d and consuming herbage-based diets supplemented with grain, the efficiency of utilizing the additional energy in the grain, as measured by the loss of energy in heat, and its partitioning to milk, did not change as lactation progressed from 110 to 560 DIM.

Effects of rearing density and water current on the respiratory physiology and haematology in rainbow trout, Oncorhynchus mykiss at high temperature

Rearing fish at high densities is considered stressful. For many species this leads to poor feed conversion, decreased feed intake and reduced growth, possibly caused by a change in energy partitioning or expenditure. To test this theory, we examined how rearing density and water current affected the respiratory physiology during rest and exercise in rainbow trout. Trout were also subjected to acute stress to examine whether rearing conditions affected the haematological response. Fish were acclimated at low (L) 25 kg m −3 and high (H) 100 kg m −3 densities, in either still water (O) at < 5 cm s −1 or in a water current (C) at a speed of 0.9 bodylength s −1, in 600 liter circular tanks for a period of 63 days. As recirculation aquaculture facilities aim to reduce water use, this is frequently associated with increasing water temperatures, particularly during summer months. For this reason experiments were performed at 19 °C. Water flow rate to each tank was maintained at 60 l min −1, to eliminate water quality and feed availability as influencing parameters. Standard metabolic rate of fish reared in a current was significantly lower (118.4 ± 2.7 mg O 2 kg −1 h −1) than for those reared in still water (133.4 ± 3.7 mg O 2 kg −1 h −1) with no effect of density. The absence of any effects of density on standard metabolic rate showed that rearing at high densities in still water did not affect energy required for maintenance in inactive, post-absorptive rainbow trout. Swimming at high speeds incurred a greater cost of transport when reared in a current. The haematological profile of fishes at rest was not affected by rearing conditions, suggesting that changes in metabolism are not related to the oxygen carrying capacity of the blood, and all treatment groups showed a haematological response to stress of similar magnitude. The absence of any effects of rearing density on the respiratory physiology in the present study emphasises the importance of water quality and food availability in farming of rainbow trout.

Energy Reallocation during and after Periods of Nutritional Stress in Steller Sea Lions: Low‐Quality Diet Reduces Capacity for Physiological Adjustments

Two groups of female Steller sea lions (groups H and P) were subjected to periods of energy restriction and subsequent refeeding during winter and summer to determine changes in energy partitioning among principal physiological functions and the potential consequences to their fitness. Both sea lion groups consumed high-quality fish (herring) before and after the energy restrictions. During restrictions, group H was fed a lower quantity of herring and group P a caloric equivalent of low-quality fish (pollock). Quantitative estimates of maintenance and production energies and qualitative estimates of thermoregulation, activity, and basal metabolic rate were measured. During summer, all animals compensated for the imposed energy deficit by releasing stored energy (production energy). Group H also optimized the energy allocation to seasonal conditions by increasing activity during summer, when fish are naturally abundant (foraging effort), and by decreasing thermoregulation capacity when waters are warmer. During winter, both groups decreased the energy allocated to overall maintenance functions (basal metabolic rate, thermoregulation, and activity together) in addition to releasing stored energy, but they preserved thermoregulatory capacity. Group H also decreased activity levels in winter, when foraging in the wild is less efficient, unlike group P. Overall, sea lions fed pollock did not change energy allocation to suit environmental conditions as readily as those fed herring. This implies that a low energy-density diet may further reduce fitness of animals in the wild during periods of nutritional stress.

Effects of low temperature stress on excitation energy partitioning and photoprotection in Zea mays.

Analysis of the partitioning of absorbed light energy within PSII into fractions utilised by PSII photochemistry (ΦPSII), thermally dissipated via ΔpH- and zeaxanthin-dependent energy quenching (ΦNPQ) and constitutive non-photochemical energy losses (Φf,D) was performed in control and cold-stressed maize (Zea mays L.) leaves. The estimated energy partitioning of absorbed light to various pathways indicated that the fraction of ΦPSII was twofold lower, whereas the proportion of thermally dissipated energy through ΦNPQ was only 30% higher, in cold-stressed plants compared with control plants. In contrast, Φf,D, the fraction of absorbed light energy dissipated by additional quenching mechanism(s), was twofold higher in cold-stressed leaves. Thermoluminescence measurements revealed that the changes in energy partitioning were accompanied by narrowing of the temperature gap (ΔTM) between S2/3QB- and S2QA- charge recombinations in cold-stressed leaves to 8°C compared with 14.4°C in control maize plants. These observations suggest an increased probability for an alternative non-radiative P680+QA- radical pair recombination pathway for energy dissipation within the reaction centre of PSII in cold-stressed maize plants. This additional quenching mechanism might play an important role in thermal energy dissipation and photoprotection when the capacity for the primary, photochemical (ΦPSII) and zeaxanthin-dependent non-photochemical quenching (ΦNPQ) pathways are thermodynamically restricted in maize leaves exposed to cold temperatures.

Biogeochemical and Biophysical Responses of the Land Surface to a Sustained Thermohaline Circulation Weakening

Abstract Biotic responses to climate change may constitute significant feedbacks to the climate system by altering biogeochemistry (e.g., carbon storage) or biophysics (i.e., albedo, evapotranspiration, and roughness length) at the land surface. Accurate projection of future climate change depends on proper accounting of these biological feedbacks. Similarly, projections of future climate change must include the potential for nonlinear responses such as thermohaline circulation (THC) weakening, which is increasingly evident in paleoclimate reconstructions and model experiments. This article uses offline simulations with the Integrated Biosphere Simulator (IBIS) to determine long-term biophysical and biogeochemical responses to climate patterns generated by the third Hadley Centre Coupled Ocean–Atmosphere General Circulation Model (HadCM3) under forced THC weakening. Total land surface carbon storage decreases by 0.5% in response to THC weakening, suggesting that the biogeochemical response would not constitute a significant climate feedback under this climate change scenario. In contrast, large regional and local changes in leaf area index (LAI) suggest that biophysical responses may constitute significant feedbacks to at least local and regional climate. Indeed, the LAI responses do lead to changes in midday direct and diffuse beam albedo over large regions of the land surface. As a result, there are large local and regional changes in the land surface's capacity to absorb solar radiation. Changes in energy partitioning between sensible and latent heat fluxes also occur. However, the change in latent heat flux from the land surface is primarily attributable to changes in precipitation that occur under forced THC weakening and not a result of the subsequent changes in vegetation.

Dietary betaine supplementation affects energy metabolism of pigs.

The effect of dietary betaine supplementation on energy partitioning in growing pigs under energy-restricted dietary conditions was assessed. The effect of betaine on the adaptation in energy metabolism of pigs over time after a change in diet and housing also was studied. Six groups of 14 group-housed barrows were assigned to one of two experimental diets: control or betaine-supplemented (0 or 1.29 g/kg of feed). Diets were corn- and soybean meal-based and were formulated to be limiting in energy content but sufficient in amino acids. The experiment comprised a 3-wk adaptation and a 3-wk experimental period. At the start of the experimental period, initial BW was 46 kg, each group of pigs was housed in a climate-controlled respiration chamber, and all pigs were subjected to a change in diet. During the experimental period, diets were diluted with 10% oat hulls. Pigs were fed at 2.5 times the energy requirements for maintenance, and during the experimental period, heat production, energy, and nitrogen balances were measured weekly. Metabolizibility of energy did not differ (P > 0.10) between diets. Averaged over the experimental period, betaine reduced heat production (P < 0.05) and energy requirements for maintenance (P < 0.10) and consequently increased energy retention (P < 0.10). Moreover, the difference in heat production between diets increased with time (P < 0.05). Similarly, the effect of betaine on the energy requirements for maintenance changed with time (P < 0.05). Maintenance requirements were similar in wk 1 and were decreased by betaine supplementation by 5.5% during wk 3 (477 vs. 452 kJ/[kg(0.75) x d]). Results of this study show that dietary betaine supplementation affects energy partitioning by growing pigs. However, based on the observed time-related changes in energy partitioning, it was concluded that dietary betaine supplementation did not influence adaptation by pigs to a change in housing and diet.

Some New Aspects of Product Distribution Functions for Three-Atom Unimolecular Reactions Performed in Beam Experiments

Energy distribution functions for the products in two classes of three-atom unimolecular reactions performed in beam experiments are derived which are much more realistic than the prior distribution functions (PDF) of Levine and Bernstein without being more complicated. In a first step, we deal with light-atom emission processes governed by short-range forces. Contrary to what is generally believed, we emphasize the applicability of statistical theory of energy distributions to such reactions in which the change in energy partitioning from critical configuration to products is weak if not negligible. In a second step, we treat within the framework of phase space theory (PST) the more usual case of processes governed by long-range forces for which, however, there exists no realistic distribution functions. Comparison with experimental results or with results of simulations and/or results obtained using PDF, PST, or the statistical adiabatic channel model (SACM) is done for the following processes: (i) C2H ...

Starvation resistance in larvae of a semiterrestrial crab, Sesarma curacaoense (Decapoda: Grapsidae)

Starvation resistance in the larvae of a semiterrestrial grapsid crab, Sesarma curacaoense De Man, 1892, was studied by point-of-no-return (PNR) and point-of-reserve-saturation (PRS) experiments. S. curacaoense shows an abbreviated larval development (only two zoeal stages) and a high degree of lecithotrophy. In complete absence of food, larval development is possible from hatching to the megalopa stage. Thus, no PNR or PRS can be given for individual zoeal stages of this species. However, late effects of early starvation indicate that lecithotrophy is only facultative in S. curacaoense zoeae: food is not essential but will be eaten when available. The endotrophic potential decreases from the first to the last larval stage: while the zoea I is entirely independent of external food sources, the zoea II shows increasingly delayed development after increased periods of initial food deprivation, and the megalopa can develop independent of food only if the preceding zoeal stages were fed continuously. Besides ontogenetic changes, significant variability was observed also in starvation resistance of larvae originating from different females. In an experiment with a particularly starvation-resistant hatch, development in the zoea II was accelerated during continuous lack of food or when starvation followed brief initial feeding periods. This effect was interpreted as a mechanism which should be specific to facultative lecithotrophy: starvation may induce a change in energy partitioning, from a preference for growth (uptake and accumulation of additional energy reserves) to accelerated development (mobilization of internal reserves, rapid termination of the planktonic larval phase). Enhanced starvation resistance in S. curacaoense zoeae compared with that in most planktotrophic marine decapod larvae is considered an adaptation that allows for retention of the larvae within a nutritionally unstable environment where the adult populations live. These ontogenetic traits corroborate the assumption that S. curacaoense may be the closest relative of an ancestral Sesarma species from which adaptive radiation of endemic Jamaican freshwater and terrestrial crabs began.

Developmental biology of Armases miersii (Grapsidae), a crab breeding in supratidal rock pools. I. Facultative lecithotrophy of larval stages

Starvation resistance in the larvae of a grapsid crab, Armases miersii, that breeds in supratidal rock pools in Jamaica was examined by point-of-no-return (PNR) and point-of-reserve- saturation (PRS) experiments. A. miersii shows an abbreviation of larval development (only 3 zoeal stages) and a high degree of lecithotrophy in the zoea I and II stages. In complete absence of food, larval development is possible from hatching to the zoea III stage. The potential for endotrophic development decreases during ontogeny: the zoea I is, in principle, entirely independent of food, without significant effects of starvation on survival; the zoea II shows significantly higher mortality under continued lack of food (since hatching) and delayed development after initial temporary starvation; the zoea III can also develop independently of food, but only if the preceding stages were fed continuously. Hence, no PNR or PRS can be given for individual zoeal stages of this species. Late effects of early feeding or starvation indicate that lecithotrophy in A. miersii is only facultative, i.e. food is not essential in the beginning of larval life, but it will be taken up when available. Conspicuous intraspecific variability in the degree of lecithotrophy was observed in larvae originating from 2 different females. In a hatch with particularly high endotrophic potential (but not in nutritionally more vulnerable larvae), development was significantly faster under starvation. This effect is interpreted as a change in energy partitioning: lack of food may induce a signal for switching from growth (accumulation of exogenous energy) to rapid mobilization of internal reserves (accelerated development, termination of the nutritionally vulnerable planktonic larval phase). Feeding after initial starvation may cause inverse effects, i.e. slower development, replacement of utilized reserves, and accumulation of additional energy for later stages. This switching should occur only in facultatively lecithotrophic larvae that have sufficient internal energy reserves to allow for this opportunistic bioenergetic strategy. Compared with most planktotrophic marine decapod larvae, the zoeal stages of A. miersii show higher starvation resistance and endotrophic potential. These ontogenetic traits are considered adaptations to breeding and larval development in a short-lived, nutritionally unpredictable habitat.

Changes in growth, appetite, food conversion efficiency and body composition in mice selected for high post-weaning weight gain on restricted feeding

This study aimed to test the hypothesis that if animals were fed the same amount over the same time period, selection of the fastest growers would result in a change in the partitioning of metabolisable energy toward more protein and less fat deposition. Two mouse lines (S1 and S2) were selected for high 5 to 9 week weight gain corrected to mean 5 week weight. Appetite variation between mice was eliminated by feeding a fixed amount to each mouse daily. After 6 generations of selection, the lines were compared with an unselected control (C) on restricted and ad libitum levels of feeding for growth rate, appetite, food conversion efficiency and chemical body composition.Realised heritabilities of 5 to 9 week gain were 0.36+ 0.05 and 0.19±0.04 for S1 and S2 respectively. Nine week weights were increased by an average of 13% on both feeding levels. Most of this increase, particularly in S2, occurred before 5 weeks and was therefore outside the period of measurement used in selection. On ad libitum feeding, selection increased food intake per unit time by 6% but there was no increase per unit body weight. Food conversion efficiency (gain/food) increased by 12%. Compared with controls at 9 weeks, 3% more of the body weights of selected mice was fat and 1% less was protein. These differences were reduced but were still in the same direction when comparisons were made at the same body weight. Thus the expected change in energy partitioning toward greater protein and less fat deposition in the S lines did not occur.It was concluded that the increased growth and energy retention in the S lines was brought about by a reduction in maintenance requirement. To achieve the desired change in energy partitioning using a similar selection scheme, higher levels of dietary protein should be fed, and some measure of protein deposition rather than growth rate used as the selection criterion.

A laboratory energy balance for the larvae and juveniles of the American lobster Homarus americanus

A laboratory energy budget was constructed for the larvae and juveniles of the American lobster Homarus americanus Milne-Edwards fed brine shrimp, Artemia saline L. Measured energy flows included ingestion, egestion, excretion of ammonia, routine and fed metabolism, growth, and production of exuvia. Digestion and assimilation were calculated and minimum ration of protein necessary to sustain larval lobsters was estimated. No change associated with metamorphosis was observed in rates of excretion, fed metabolism, and production of exuvia. Routine metabolism is not significantly higher for larvae than for juveniles. Growth changes from exponential in larvae to a slower increase in post-larvae. Consumption reflects changes in other variables. Changes in energy partitioning and energetic efficiencies associated with metamorphosis are largely due to change in rate of growth.