Changes In Crude Protein Content Research Articles

PSV-12 Microbial community structure of dairy cattle is stable through oscillating crude protein inclusion

Abstract Diet has an important role in shaping the structure of rumen microbial communities. However, little is known about the interaction between dietary crude protein (CP) level and CP feeding pattern on the rumen microbiome of dairy cows. Here, the objective was to evaluate the influence of CP level (low protein [LP], 13.8%; high protein [HP], 15.5% of dry matter [DM] basis) and CP feeding pattern (OF = oscillating, SF = static) on the composition and diversity of the rumen microbiome of lactating dairy cows using 16S rRNA gene amplicon sequencing. Rumen-cannulated Holstein cows (n = 8) in mid- to late-lactation were assigned to a 2 x 2 factorial experiment consisting of 4 experimental periods of 28 d. Each experimental period had an adaptation phase to the diet (24 d) and a sampling phase (d 25 to 28). The treatments with oscillating feeding alternated diets every 48 h to vary CP above and below the mean CP level (OF-LP = 13.8 ± 1.8%; OF-HP = 15.5 ± 1.8% CP of DM) whereas diets were constant in the static feeding (SF-LP = 13.8%; SF-HP = 15.5% CP of DM). According to the NASEM (2021) model predictions the LP diet supplied 94% of the microbial protein requirement while the HP diet exceeded (104%) requirement. Rumen samples were collected 4 h after feeding through a rumen cannula and amplicon libraries were generated targeting the V4 region of the 16S rRNA gene. The amplicon sequences were analyzed in qiime2. Sequences where trimmed based on their quality and then grouped in ASVs using DADA2. The ASVs were used to analyze the composition and diversity of the rumen microbiome in each treatment. Alpha diversity of the rumen microbiome did not change across treatments (Kruskal Wallis, P > 0.05), but Pielou’s evenness increased by 0.01 units (Kruskal Wallis, P = 0.04). These results support the hypothesis that the rumen microbiome is stable to oscillating CP feeding patterns and compensates for the changes in crude protein content in the diet. Further research is required to understand the dynamics of this compensation and what other metabolic processes in the ruminant contribute to maintaining production under an oscillating diet.

Seasonal ammonia emissions from an intensive beef cattle feedlot in Victoria Australia

Ammonia (NH3) emitted from concentrated animal feeding operations can cause environmental and health problems, and indirectly contribute to greenhouse gas emissions. Cattle feedlots are known to be large sources of NH3, but few studies have documented seasonal emissions from Australian feedlots. We conducted two field campaigns to measure NH3 emissions from an intensive beef cattle feedlot in southeast Australia, and these results were combined with previous measurements at the same feedlot to document seasonal variations in emissions and to derive annual feedlot emission factors (EFs). Emission rates were calculated with an inverse dispersion modelling (IDM) technique, based on NH3 concentrations measured at the feedlot with open-path lasers (OPLs). The average area emission rates in spring, summer, autumn and winter were 90.5, 167.4, 96.2 and 86.8 μg NH3 m−2 s−1 from the cattle pens, and 22.5, 18.1, 7.7 and 20.7 μg NH3 m−2 s−1 from the manure stockpile area, respectively. The total per-animal EFs ranged from 126.0 (autumn) to 190.2 g NH3 animal−1 d−1 (summer), representing a loss of 47.5–64.6% of the fed N. Seasonal variations in emissions were related to air temperature. Slight changes in crude protein content of the cattle diet may also have impacted seasonal variability. Taking seasonal variations into consideration, the average feedlot EF was 160.4 g NH3 animal−1 d−1, with 90% of the emissions coming from the cattle pens. Extrapolating the EF to all feedlot cattle in the country, the direct NH3 emissions from Australian feedlots amount to 65.2 Gg NH3 annually, or 3.7% of the national total. Our study benchmarks seasonal and annual EFs and N losses for Australian commercial feedlots, and provides a baseline for extrapolating the impacts of mitigation efforts.

Changes in crude protein content and amino acids profile in Ginkgo biloba nuts during storage

ABSTRACT Storage is an important issue for ginkgo nuts industry because it is critical to enabling a year-round supply and the next year of cultivation. An investigation into the crude protein content and amino acids profile in ginkgo nuts after different storage times in terms of varieties was carried out in this work. The crude protein content was generally increased during storage while an opposite trend was observed for the concentration of total amino acids. The concentrations of almost all amino acids were more or less decreased except Cys, which was increased 42.13% after 5-month storage, and showed a significant positive correlation with storage time. The effect of storage seemed to be variety-dependent that the variety Tancheng-300 was less prone to fluctuations in amino acids profile amongst the varieties.

Evaluate the Effect of Processing of Chhattisgarh Local Paddy Variety to Flaked Rice (POHA) on Proximate, Chemical and Functional Characteristics

The present study was undertaken to study the evaluate the effect of processing of Chhattisgarh local paddy variety to flaked rice (Poha) on proximate, functional and chemical characteristics. In the proximate analysis, change in crude fat content from paddy 3.20% to 0.96% for thick and 1.00% for thin flaked rice. The change in Crude fiber content from paddy to flaked rice was found to be 2.00% to 1.85% for thick and 1.79 for thin flaked rice. Similarly, change in crude protein content from paddy to flaked rice was found to be 4.23% to 4.04% for thick and 3.76 for thin flaked rice and change in ash content from paddy to flaked rice was found to be 1.00% to 1.35% for thick and 1.24% for thin flaked rice. Chemical properties, show that amylose content from paddy to flaked rice 19.27% to 18.19% for thick and 18.26% for thin flaked rice. Similarly, change in starch content from paddy to flaked rice 74.15% to 73.89% for thick and 73.47% for thin flaked rice. Functional properties, show that Water absorbing index (WAI) from paddy to flaked rice 2.19 g/g to 5.18 g/g for thick and 5.05 g/g for thin flaked rice. Similarly, water solubility index (WSI) from paddy to flaked rice 3.25% to 4.86 for thick and 5.09% for thin flaked rice.

Effects of dietary crude protein and calcium/phosphorus content on growth, nitrogen and mineral retention in broiler chickens

This experiment was conducted to examine the effect of three levels of crude protein (CP) (NRC, 15% more than NRC and 15% less than NRC) and three levels of Ca and available P (Av. P) (NRC, 15% more than NRC and 15% less than NRC) on performance of broilers from hatching until 21 days of age. The experimental design was a completely randomized design (CRD), with a 3 × 3 factorial arrangement of nine dietary treatments. Each treatment combination had four replicate pens (10 birds per pen). Results of this experiment showed that CP content had no significant effect on feed and water intake. However, body weight gain (BWG) significantly reduced and FCR increased (P < 0.05) by 15% change in CP content of diet. Fifteen percent increase in mineral content of diets had no significant effect on feed intake. However, increase in Ca and Av. P significantly increased BWG and resulted in an improved feed conversion ratio (FCR) and increased water intake. There was an interactive effect of CP by Ca and Av. P levels on feed intake, BWG and FCR. A change in CP or mineral content of the diets had no significant effect on blood parameters except for potassium concentration (K + ). Reducing CP, Ca and Av. P content of the diet significantly (P < 0.001) increased P, Ca and N retention. Reduction in CP content of the diet led to a decrease in length, P and tibia bone ash. There was an interactive effect of CP by Ca and Av. P on the retention of Ca, N and ash of tibia bone.

Effect of Household Processing on the In Vitro Bioavailability of Iron in Mungbean (Vigna Radiata)

Mungbean (Vigna radiata) is a major source of energy and protein in developing countries, especially for the vegetarian population. Improvement of the bioavailability of iron in mungbean by common household processes could make a significant contribution to the nutrition of people in countries where iron-deficiency anemia is widespread. The study was conducted to determine the effect of common household processes on nutritional and antinutritional factors as well as in vitro bioavailability of iron in mungbean. Mungbean was subjected to various domestic processes such as dehulling, pressure cooking, germination, and fermentation. The effects of these processes on proximate composition, antinutritional factors (phytin phosphorus, polyphenols, and neutral detergent), and iron, including ionizable iron, were determined. No significant change in crude protein content was observed. There was a significant (p < or = .05) increase in fiber content after germination. Ash content decreased significantly (p < or = .05) after all processing methods. The processing methods resulted in a significant (p < or = .05) reduction in phytin phosphorus and polyphenols. Pressure cooking significantly (p < or = .05) decreased the neutral detergent fiber, whereas fermentation and germination increased it. The phytate:iron molar ratio of processed mungbean revealed that the maximum reduction was in germinated and fermented samples. Ascorbic acid content increased significantly (p < or = .05) after germination. The in vitro bioavailability of iron in raw mungbean was 7.32%. All of the processing methods resulted in an increase in iron bioavailability in vitro; the maximum bioavailability was in germinated cooked mungbean (12.52%), followed by fermented cooked mungbean and germinated raw mungbean (both 11.04%). Suitable processing techniques can improve the in vitro bioavailability of iron from mungbean. If mungbean products with enhanced iron bioavailabilty are developed, they could help improve the iron status of the population.

Effects of spring grazing on dryland perennial ryegrass/white clover dairy pastures. 1. Pasture accumulation rates, dry matter consumed yield, and nutritive characteristics

A 3-year experiment (September 1999–August 2002) was conducted in south-western Victoria to determine the impact of spring grazing on pasture accumulation rates, dry matter (DM) consumed yield (estimate of DM yield), and pasture nutritive characteristics [metabolisable energy (ME), crude protein (CP), neutral detergent fibre (NDF), and water-soluble carbohydrates (WSC)] of a perennial ryegrass (Lolium perenne L.)–white clover (Trifolium repens L.) pasture. Spring grazing treatments, applied annually from September to November, were based on ryegrass leaf development stage with high (HF), medium (MF), and low (LF) grazing frequency being 2-, 3-, and 4-leaf stage, respectively, and post-grazing height as the grazing intensity with high (HI), medium (MI), and low (LI) grazing intensity being 3, 5, and 8 cm, respectively. Five combinations were used: HFHI, LFHI, MFMI, HFLI, and LFLI. A sixth treatment, rapid grazing (RG), maintained pasture between 1500 and 1800 kg DM/ha by grazing weekly during spring, and a seventh and eighth treatment, simulating forage conservation for early-cut silage (lock-up for 6–7 weeks; SIL) and late-cut hay (lock-up for 11–12 weeks; HAY), were also included. For the remainder of the year, all plots were grazed at the perennial ryegrass 3-leaf stage of growth, or when pasture mass had reached 2800 kg DM/ha, and grazed to a residual height of 5 cm. On average, pasture accumulation rates ranged from &lt;5 (February–March) to 100–110 kg DM/ha.day (September–October). Overall, SIL resulted in a lower accumulation rate than all other treatments. High spring grazing frequency (including RG) treatments led to more grazing events than medium and low spring grazing frequency treatments. In Years 1, 2, and 3, DM consumed ranged from 9.7 (HAY) to 16.3 (RG), 4.2 (HAY) to 10.1 (HFHI), and 7.3 (SIL) to 10.9 t DM/ha.year (HAY), respectively. HAY resulted in a lower pasture ME content than SIL, HFHI, and LFHI spring grazing, and LFLI spring grazing resulted in a lower pasture ME content than all other treatments except HAY. HFHI grazing resulted in an increase in ME content over time, whereas the rate of increase in ME content over time was higher for LFLI spring grazing than for HAY, RG, and HFLI spring grazing. For all treatments, average pasture ME content ranged from 9.4 (January–February) to 11.4 MJ/kg DM (September). HAY resulted in a lower CP content than all treatments except LFLI grazing. RG resulted in no change in CP content over time. For all treatments, average pasture CP content ranged from a low of 11–14 (January–February) to a high of 24–28% DM (August–September). LFLI grazing resulted in a higher NDF content than HFHI, LFHI, MFMI, and HFLI grazing, while RG resulted in a lower NDF content than LFHI, MFMI, and HFLI. For all treatments, average pasture NDF content ranged from a low of 48–55 (August–September) to a high of 58–62% DM (January–February). All treatments resulted in an increase in pasture WSC content over time. The results demonstrate that frequent and intense grazing management (e.g. HFHI and RG) during spring is important in maintaining high pasture DM yields. Results also indicate positive pasture nutritive characteristic (ME, CP, and NDF) gains with more frequent spring grazing than with infrequent spring grazing. No treatment effect was observed for WSC content.

Dry matter yield, forage quality and persistence of tall fescue (Festuca arundinacea) cultivars compared with perennial ryegrass (Lolium perenne) in a subtropical environment

The dry matter (DM) yield, plant persistence and forage quality of tall fescue (Festuca arundinacea) and perennial ryegrass (Lolium perenne) were compared in the subtropical environment of southern Queensland, Australia. The field study was conducted under irrigation with pure, nitrogen fertilised stands of 10 commercial tall fescue cultivars (Advance, AU Triumph, Bombina, Cajun, Dovey, Maximise, Midwin, Torpedo, Quantum and Vulcan), 3 experimental cultivars (ITF 97010, ITF 97020 and PWF 29) and Dobson perennial ryegrass. From July 1997, plots were defoliated at 4-week intervals for 3 years. Changes in crude protein content and in vitro DM digestibility (IVDMD) were determined at 1, 2, 3, 4, 6 and 8 weeks post-defoliation in October (spring) 1997, January (summer), March (autumn), June (winter) and September (spring) 1998.Some cultivars of irrigated tall fescue were shown to be better adapted to a subtropical environment than perennial ryegrass. After 3 years, cumulative DM yields were in excess of 30 t/ha for Dovey, Quantum, ITF 97010, AU Triumph and Cajun tall fescue compared with 12 t/ha from Dobson perennial ryegrass swards.Plant development had a considerable influence on crude protein content and IVDMD of tall fescue and perennial ryegrass, more so than the length of the regrowth period. As plant tissue matured, the forage quality during spring declined linearly for crude protein content and for IVDMD (1998 only), and declined exponentially for IVDMD during spring (1997), summer, autumn and winter (1998). Quality losses may be minimised if tall fescue cultivars are defoliated every 2–3 weeks during spring and summer and every 3–4 weeks during autumn and winter.

Plant Phenology and the Benefits of Migration in a Temperate Ungulate

Seasonal changes in crude protein content of graminoids and herbs grazed by red deer were monitored from 1 May, or as soon as snow melt exposed the vegetation, until 15 October at five sites along an altitudinal gradient from coast to inland. Crude protein declined exponentially with time at all sites, but declined most rapidly from initially higher values at inland locations at high elevations. As a result crude protein was positively correlated with altitude and distance from the coast in early summer and negatively correlated in autumn. The relationships between protein content, date and altitude were used to estimate the quality of the diet of twelve radio-collared female red deer that migrated to summer ranges in the mountains

The effect of cutting times on goat’s rue (Galega orientalis Lam.) leys

The effect of four different cutting times, both in spring and autumn, on goat’s rue was studied at Viikki Experimental farm of the University of Helsinki in 1983—89. Goat’s rue showed good persistence. The plots remained in good condition, the average yield being even in the sixth year 9000 kg DM per hectare. The development of goat’s rue starts early in the spring. The growth rate and development of CP content are similar to those of red clover. The development of CF is, however, more similar to grasses. Thus, the crude fiber content limits the cutting times of goat’s rue more than the changes in crude protein content. The most suitable cutting time in spring is at the beginning of flowering in mid-June, and in autumn during the second week of September. With this management a yield of 8360 kg DM per hectare per year was reached during the experimental years. The pooled CP content was 19.9 % and the CP yield was 1660 kg/ha. The CF content was in the first cut 27.9 % and in the second cut 29.1 %. The amount of weeds in the five to six year leys was 12—18 %.