Chamomile Plants Research Articles

Rhexocercosporidium matricariae sp. nov.: A New Pathogen on the Medicinal Plant Matricaria recutita.

A new disease was observed on chamomile plants (Matricaria recutita) in various commercially cultivated fields in 2016 and 2017 in Germany. From symptomatic stems and leaves, the fungal species described here was isolated repeatedly. Koch's postulates using in planta experiments were fulfilled by inoculation of chamomile plants proving the fungus to be the causal agent of the disease. Morphological studies and phylogenetic analyses using internal transcribed spacer, large subunit, and translation elongation factor-1α sequences suggested that the fungus represents a new species within the genus Rhexocercosporidium (Helotiales). The data are presented together with a description of the growth parameters and comprehensive illustrations of the new species, Rhexocercosporidium matricariae. All species so far assigned to Rhexocercosporidium are compared and discussed. The combination Rhexocercosporidium microsporum is validated.

Bплив ранньовесняних строків сівби насіння ромашки лікарської на польову схожість та виживання рослин в умовах правобережного лісостепу України

An important place in the effective technology of growing medicinal chamomile (Matricaria recutita L.) is occupied by the early spring sowing period. Therefore, they provide a dense emergence of seeds, as evidenced by many conducted scientific studies in the field of plant breeding. The article presents the results of experimental research on the effect of early spring sowing dates of medicinal chamomile on field seed germination and plant survival. It was established that medicinal chamomile plants depended on weather and climatic conditions, sufficient amount of moisture in the soil, soil crust, which as a result led to variation in the passage of growth and development phases, and therefore, a question arose in the study of agrotechnical techniques aimed at the productivity of medicinal raw materials. The cultivation of tetraploid of medicinal chamomile variety Perlyna Lisostepu and Zloty Lan in the conditions of the Right-Bank Forest-Steppe of Ukraine during the period of research (2018-2023) in the early spring sowing period was studied. It was established that the investigated varieties of medicinal chamomile had the highest field germination of seeds due to the content of productive moisture. The highest field seed germination rates of 74.16% were recorded during the early spring (15-18.04) sowing period of the Perlyna Lisostepu variety, and the Zloty Lan variety was 72.68%, respectively. According to six-year research data, the highest field seed germination of 68.00% on the control variant (1-3.04) according to the sowing dates was noted in the Perlyna Lisostepu variety, and the lowest 67.00% – in the Zloty Lan variety. The maximum rate of plant survival at the end of the growing season was 88.96%, which was obtained in the variant of the early spring (15-18.04) sowing period of the Perlyna Lisostepu variety, the minimum rate was 69.15% in the control (1-3.04) of the Zloty Lan variety. The germination rate on average over six years exceeded the control by 6.14%, and the survival of chamomile plants on average over the years of research exceeded the control option by 1.5%. The difference in factors was insignificant (2-3%)

Biological control of powdery mildew on chamomile plants

Biological control of powdery mildew on chamomile plants

Gallic acid affects chamomile flower’s essential oil

Chamomile essential oil is used in the food and medicinal industries. Gallic acid has been identified as one of the most significant biological elicitors. At dosages of 0, 5, 10, 15, and 20 mg/L, Gallic acid was sprayed on chamomile plants. The yield of flowers and essential oils in dry flowers were assessed. The averages of data were examined statistically using one-way analysis of variance. Oxygenated sesquiterpenes were a major chemical group, while α-bisabolol oxide A, α-bisabolol and chamazulene were the three main components of essential oil. When administered 15 mg/L Gallic acid, plants produced their most flowers and had the highest quantities of the essential oil, α-bisabolol oxide A, α-bisabolol and chamazulene. Essential oil extracted from untreated control plants had the highest concentration of oxygenated sesquiterpenes. It can be infer that Gallic acid increases the production of essential oil and alters its chemical constituents which alter its biological activity.

Morphological characterization, fungicidal alternatives and biological control of Peronospora farinosa on chamomile

BackgroundChamomile (Matricaria chamomile L.) is one of the very important medicinal plants worldwide, particularly in Egypt. The downy mildew disease is considered one of the most important diseases of chamomile. Peronospora farinose was identified as the causal pathogen of downy mildew of infected chamomile plants, collected from Fayoum Governorate, Egypt, during 2022.ResultsEfficiency of some fungicidal alternatives for management of downy mildew disease on chamomile leaves was evaluated in vitro studies using detached leaves technique. Each of the biocide, Bio-Cure F and Thyme oil emulsion treatments, before and after the fungal inoculation, respectively, was the best effective treatments, which reduced disease incidence to the lowest percentages. However, the treatment of Bio-Cure F before the fungal inoculation had the superiority for reducing disease severity to the lowest percentage, followed by Thyme oil emulsion, after the fungal inoculation. On the other hand, under greenhouse conditions, the best treatment reduced disease incidence and disease severity to the lowest percentages was Thyme oil emulsion, followed by Bio-Cure F, after and before the fungal inoculation, respectively. Total sugars content was obviously lower in treated chamomile plants before and after the fungal inoculation than in untreated controls. On the contrary, free phenols were obviously higher in treated chamomile plants before and after the fungal inoculation than in untreated controls.ConclusionThe present study indicated the possibility of using Bio-Cure F (biocide) and Thyme essential oil emulsion as spraying treatment against P. farinosa on chamomile plants.

Influence of antimicrobial substances of plant origin on the sensitivity of bacterial biofilm to antimicrobial drugs

The widespread antibiotic resistance is associated with the formation of special forms of microbial communities — bacterial biofilms with increased resistance to the impact of various physicochemical factors, including antimicrobial drugs. In this regard, the investigation of antibacterial agents, including herbal preparations that could affect bacterial biofilms, seems to be of current interest. The aim of this work was to identify antibacterial substances of plant origin that can most effectively affect bacterial biofilms. The effect of antibacterial decoctions of lingonberry, sage, and chamomile plants on the sensitivity of bacteria to antimicrobial drugs was assessed. The susceptibility of bacteria to antimicrobial drugs, when combined with herbal decoctions, was determined by the disk-diffusion method. The morphophysiological properties of biofilms were illustrated by light and scanning electron microscopy. The ability of lingonberry, sage, and chamomile decoctions to increase the sensitivity of bacteria to antimicrobial drugs of predominantly bactericidal pharmacological action was revealed. Decoctions of medicinal plants prevented the formation of complete bacterial biofilms. The obtained results indicate the antibiofilm activity of lingonberry, chamomile and sage decoctions, which contribute to a decrease in the level of antibiotic resistance of bacteria and a decrease in the load during antimicrobial therap.

Article Review: Medical Plants: Their Compounds and the Biotechniques for Identifying and Separation of Them

It was possible through this study to give a brief overview of the most important pharmaceutical compounds, which included glycosides, alkaloids, terpenes and their plant sources, which encourages the adoption of various separation techniques protocols to obtain multiple types of pharmaceutical compounds of high pharmacological value from medicinal plants compared to its standard compounds and thus the possibility of using it medically to cure many diseases and dispense with medicines and chemotherapy with multiple side effects and interactions. The study dealt with garlic, ginger and chamomile plants and their pharmaceutical compounds as examples of medicinal plants known since ancient times, research has confirmed its health benefits, as it supports normal body functions and the immune system.

Segetal vegetation in Matricaria recutita (l.) crops according to protection methods and its influence on growth dynamics and inflorescence formation

Aim. After all, the quality of medicinal raw materials, and subsequently the preparations made from them, directly depend on the number of weeds present in the crop. Currently, producers of agricultural products who cultivate medicinal plants, namely medicinal chamomile, are faced with such a question as the protection of medicinal crops from harmful organisms, especially weeds, during cultivation technology. The research was aimed at establishing the dependence of the growth dynamics of medicinal chamomile plants and the productivity of inflorescences on the degree of weediness of crops by segetal plants. Methods. The weed protection system included agrotechnical, chemical and integrated methods of control. Results. It was found that the most common weeds in the chamomile agrophytocenosis were Centaurea cyanus L., Polygonum patulum, Convolvulus arvensis L., Avena fatua, Echinochloa crus-galli L., Galinsoga parviflora Cav., Chenopodium album L., Galium aparine L., Raphanus raphanistrum L., Barbarea vulgaris R.Br. It was established that the use of the autumn sowing period of chamomile contributed to more effective suppression of weeds, since a certain number of weeds die in winter at low temperatures.The average weediness after harvesting the predecessor before preparing the field for sowing medicinal chamomile was 115–123 pcs./m2. Agrotechnical protection measures reduced the number of weeds to 42–50 pcs./m2, the use of herbicide, respectively – 17–18 pcs./m2, integrated protection – 12–15 pcs./m2. Conclusion. We have established that the Perlyna Lisostepu variety has significant resistance to weeds. As for the dependence of the growth dynamics and productivity of chamomile on the implementation of weed control measures, the combination of all measures, that is the use of only an integrated method, makes it possible to best deal with harmful plants namely to reduce their number on average over the years of research to 12 pcs./m2 and obtain a yield of 1.5 t/ha of raw mass of inflorescences.

Determination of Critical Weed Competition Period in Chamomile (Matricaria chamomilla L.) Production at Wondo Genet, Southern Ethiopia

The study was carried out at Wondo Genet to determine the critical weed competition period for growth, yield, and yield components of the chamomile plant. The weed competition duration had a significant influence on plant height, the number of primary branches/plants, fresh stem yield/plant, fresh leaves yield/plant, fresh and dry flower/plant, fresh and dry flower yield/ha, essential oil content (v/w) and yield kg/ha. The highest fresh and dried flower yield (3780.10 kg/ha) and (798.32 kg/ha) was recorded from weed-free check followed by weed-free for 60 DACE (Days After Crop Emergence) (3652.10 kg/ha) and (789.43 kg/ha), respectively. The maximum essential oil content and yield were recorded from the weed-free check (0.79v/w) and (6.25 kg/ha) whereas the minimum was in the weedy-check (0.10 v/w) and (0.15kg/ha), respectively. The yield losses of chamomile were estimated based on the fresh and dry flower and essential oil yield per hectare. Thus, the highest fresh and dry flower yield losses were recorded from the weedy-check (83.58%) and (81.05%) while the lowest was from weed-free check (0.00%), respectively. The highest essential oil yield loss was also recorded from the weedy for 60DACE (97.92%) followed by weedy-check (97.60%). To determine the beginning and the end of the critical period of crop-weed competition 5 and 0% acceptable yield loss levels were used. Therefore, to reduce the yield losses of chamomile by more than 10% and higher economic return, plants must be kept weed-free within 40 to 60DACE to reduce the risk of economic yield losses as it is the critical period of weed-crop competition in chamomile plants.

A Pivotal Role of Chitosan Nanoparticles in Enhancing the Essential Oil Productivity and Antioxidant Capacity in Matricaria chamomilla L.

Chitosan is a biopolymer with several biological and agricultural applications. Recently, development of chitosan nanoparticles (CSNPs) adds additional value by further using it as an eco-friendly biostimulant. Therefore, the impact of CSNPs foliar application on the growth, essential oil productivity and antioxidant capacity of chamomile was investigated. Treatments comprised 0, 100, 200, 300 and 400 mg L−1 of CSNPs applied to plants as a foliar spray. CSNPs foliar application improved the growth and productivity of chamomile plants. Relative to the control, the flower yield was increased by 52.10 and 55.74% while the essential oil percentage was increased by 57.14 and 47.06% due to CSNPs at 300 mg L−1 during the two seasons of study. Moreover, CSNPs enhanced the photosynthetic pigments, total soluble sugars and N, P and K percentages. Interestingly, CSNPs increased the antioxidant capacity as measured by total phenolics and the antioxidant activity (DPPH). Collectively, it is suggested that CSNPs might be a promising eco-friendly bio-stimulant and it could be an alternative strategy to improve the productivity, quality and decrease the production cost of chamomile and possibly some other medicinal species.

Comparative study of wild chamomile plants from the north-west of Morocco: Bioactive components and total antioxidant activity

Morocco presents numerous plants due its localization between the Mediterranean Sea and Atlantic Ocean and the variability of its mountainous terrain, with a rich range of medicinal plants. Cladanthus mixtus (L.) Oberpr. & Vogt and Matricaria chamomilla L., two Asteraceae (Compositae) from Beni Hassane region in north-western Morocco are both used in traditional medicine for the treatment of different ailments. The phenolic and flavonoid contents of roots, stems, leaves, and flowers of both species were determined by using the Folin Ciocalteu and the aluminum chloride methods, respectively. Antioxidant activity of their aqueous and organic compounds was performed applying the robust and widely used method ABTS radical scavenging assay. C. mixtus had a higher phenolic content (115.25 mg GAE g -1 DW) than M. chamomilla (82.99 mg GAE g-1 DW). However, flavonoid content of both plants was very close (33.53 and 32.45 mg QE g-1 DW in M. chamomilla and C. mixtus, respectively). The total phenolic and flavonoid content was high in flowers of both plants. Generally, for all the studied organs of the chosen plants, hydrophilic antioxidant activity was significantly higher than the lipophilic antioxidant activity. The correlation results show that these bioactive components in both plants are the main factor influencing the antioxidant activity. Key words: Cladanthus mixtus, Matricaria chamomilla, Asteraceae, phenolic content, flavonoid content, antioxidant activity.

Mitigation of Heat Stress Effects on Chamomile and its Essential Oil Using Melatonin or Gibberellic Acid and some Agricultural Treatments.

Temperature change risk in horticultural crops includes modified phonology (timing) of leafing, flowering, and harvest. The yield of essential oil of chamomile depends on some factors such as the plant region's environmental conditions. Therefore, this study was carried out during two successive growing seasons on chamomile plants grown under either region; northern Egypt and East of Cairo to reduce the impact of heat stress on growth, oil yield and its components which, the seedlings had been cultivated at half of October and the half of November and sprayed with either gibberellin or melatonin. We observed that spraying melatonin or gibberellins improved growth characteristics compared to control. There was a clear superiority, there was a clear superiority of melatonin on chamomile plants grown under Nubaria which improved most of the previous characteristics. Chamomile seedling can be grown from mid-October to mid-November, so it can be harvested in March before the heat rises and maintains the resulting oil quality maintains the resulting oil quality. The main component of Chamomile oil is (Bisabolol oxide A) and was higher under the conditions of Nubaria when seedling was treated with gibberellin followed in the lowest order by treatment with melatonin, but it was the opposite under the conditions of Sekam region. The second component was (Bisabolol oxide B).

Soil mites inhabiting chamomile and marigold plants under two different cultivations at Fayoum governorate

Soil mites inhabiting chamomile and marigold plants under two different cultivations at Fayoum governorate

Effects of chitosan and BABA foliar application on flowering and chemical characteristics of German chamomile ‘Bode-gold’

The effect of chitosan and D,L β aminobutyric acid (BABA) at rates of 10, 20 and 40 ppm on the flowering, and chemical characteristics of German chamomile (Matricaria recutita L. cv. Bode-gold) was studied. Chitosan treatments with different concentrations significantly increased flower number per plant, flower head fresh weight, flower head dry weight, and yield of dry flowers per fed in comparison with all other treatments including the control (untreated plants). Plants treated with BABA had bigger flower head diameter compared to the other treatments including the control. The highest concentration of BABA (40 ppm) gave the highest volatile oil content in flowers (2.27 and 2.23 % during both seasons) with increasing rates of 32.59 and 29.59 % over that of the control during both seasons, respectively. Chitosan at 40 ppm followed by BABA at 40 ppm exhibited a significant increase in oil yield per fed comparing to the control with no significant differences between both of them ppm during both seasons. The highest percentage of chamazulene as the main active component was recorded in the volatile oil extracted from chamomile plants treated with BABA at 20 ppm. This investigation suggests further studies on using the foliar application of chitosan at a rate of 40 ppm to increase the quality of chamomile flower parameters and BABA at a rate of 40 ppm to improve the percentage and yield of volatile oil.

Ethylene Induction of Non-Enzymatic Metabolic Antioxidants in Matricaria chamomilla.

Phytochemical investigations of Matricaria chamomilla L. (Asteraceae) stated the presence of several compounds with an established therapeutic and antioxidant potential. The chamomile non-enzymatic antioxidant system includes low molecular mass compounds, mainly polyphenols such as cinnamic, hydroxybenzoic and chlorogenic acids, flavonoids and coumarins. The objective of this work was to evaluate the role of the non-enzymatic antioxidant system after stimulation by ethylene in tetraploid chamomile plants. Seven days of ethylene treatment significantly increased the activity of phenylalanine ammonia-lyase, which influenced the biosynthesis of protective polyphenols in the first step of their biosynthetic pathway. Subsequently, considerable enhanced levels of phenolic metabolites with a substantial antioxidant effect (syringic, vanillic and caffeic acid, 1,5-dicaffeoylquinic acid, quercetin, luteolin, daphnin, and herniarin) were determined by HPLC-DAD-MS. The minimal information on the chlorogenic acids function in chamomile led to the isolation and identification of 5-O-feruloylquinic acid. It is accumulated during normal conditions, but after the excessive effect of abiotic stress, its level significantly decreases and levels of other caffeoylquinic acids enhance. Our results suggest that ethephon may act as a stimulant of the production of pharmaceutically important non-enzymatic antioxidants in chamomile leaves and thus, lead to an overall change in phytochemical content and therapeutic effects of chamomile plants, as well.

Effect of Saline Irrigation Water, Gibberellic Acid(GA3) and Biofertilizers on Growth, Flowers Yield and Oil Production of Matricaria Chamomilla, L.plants

The present study was conducted to investigate the effects of salinity, gibberellic acid (GA3) and biofertilizers on growth, flowering and oil yield of Matricaria chamomilla, L. var. German chamomile plants. The experiment was designed as a split-split plot design in three replicates. The main plot represented different concentrations of saline irrigation water ( tap water , 3 and 6 dSm-1 ) . The sub-plot was gibberellic acid (GA3) as foliar application at four levels 0.0, 100 and 200 mg L-1. The sub sub-plot represented bio-fertilizers [nitrobine + phosphorein 1:1 w/w] at rates of 0.0, 5.0 and 10.0 g/ plant . The best treatment due to these combination was resulted from irrigation plants with fresh or moderate saline water at EC 3 dSm-1 combined with spraying with all levels of GA3 combined with biofertilizer application at 5.0 and 10.0 g/ plant on growth and flowering characteristics. Maximum oil percentage resulted from irrigation with saline water and application the highest levels of GA3 and biofertilizer. Combination of fresh water, 200 mg L-1 GA3 and 10 g nitrobein + 10 g phosporein per pot produced the maximum essential oil yield /plant . Key words: Medicinal and Aromatic Plants, Chamomile, Matricaria chamomilla, L., salinity,

Infestation of Field Dodder (Cuscuta campestris Yunck.) Promotes Changes in Host Dry Weight and Essential Oil Production in Two Aromatic Plants, Peppermint and Chamomile.

Peppermint (Mentha piperita L.) and chamomile (Chamomilla recutita (L.) Rausch.) are aromatic plants with considerable economic value. These plants and their essential oils are used in medicine, cosmetics, and the food industry. One of the main limiting factors in peppermint and chamomile commercial cultivation is weed competition since weeds are able to decrease both oil amount and biomass yield. The purpose of the present study was to determine the effect of parasitism by field dodder (Cuscuta campestris Yunck.) on peppermint and chamomile dry weight and their essential oil yield and composition. Essential oils from both noninfested and infested peppermint and chamomile plants were obtained by hydrodistillation and characterized chemically by gas chromatography (GC) coupled with mass spectrometry (MS). The amount of dry matter accumulated by peppermint and chamomile plants infested by field dodder was lower (25% and 63%, respectively) compared to noninfested plants. Essential oil yield increased for peppermint (3.87% (v/w) and 3.63% (v/w)), but decreased for chamomile (0.2% (v/w) and 0.5% (v/w)) both from infested and noninfested plants, respectively. The oil composition profile significantly differed in terms of content. In peppermint plants, field dodder infestation increased menthone content by 23%, and decreased the content of both menthol by 11% and pulegone by 67%. Furthermore, δ-cadinene was detected only in oil extracted from infested peppermint plants. Compared to peppermint, chamomile plants were significantly more affected by field dodder in terms of essential oil yield, as well as oil composition and plant dry weight. In chamomile plants, (E)-dendrolasin was detected in the oil of noninfested plants, and 1,4-dimethyl-7-(1-methylethyl)-azulen-2-ol was detected only in the oil of infested plants.

Dependence of field germination and survival of chamomile plants on vegetation and agro-technical factors

The purpose of the research was to study the essence of ensuring high germination of seeds and survival of chamomile plants depending on the variety, seeding rate and sowing time in the Right-bank Forest Steppe. Chamomile plants have been found to be signifi cantly responsive to the soil and climatic conditions of the region, which has led to uneven seedlings, so it is important to investigate agrotechnological techniques aimed at increasing of seed germination energy and simultaneity of sprouts. The agrobiological features of medicinal plants cultivation in the conditions of the RightBank Forest Steppe, in particular, of chamomile (Matricaria recutita), which is most used in offi cial and folk medicine among medicinal plants due to the presence of various biologically active substances in it, were investigated. The study was conducted with samples of chamomile plants in the area of the Right-bank Forest-Steppe of Ukraine (field of study (IE Prudyvus), a branch of the department of Podilsky State Agrarian and Technical University), taking into account all the requirements of the methodology of the research case. The following results were obtained: the period of vegetation of chamomile was mainly dependent on the sowing time, the duration ranged from 83 to 240 days; fi eld germination of chamomile plants in the average for 2017‒2019 was in the range of 71‒89 %; the maximum rate of plant survival at the end of the growing season was 93 %, which was obtained on the variant of autumn sowing with a sowing rate of 6 kg/ha in the Perlyna of the Forest-steppe, the minimum indicator was noted 72 % for the summer sowing with a sowing rate of 8 kg/ha in the Bodegold variety; the germination and survival rate exceeded the control by 10‒13 %. Today, this crop is quite widespread and is cultivated independently of the fi rst established cultivation areas and existing research stations of medicinal plants, and in particular, as a culture of a wide range of use of raw materials both in Ukraine and abroad. Key words: chamomile, variety, seeding rate, sowing period, fi eld germination, plant survival, vegetation factors.

Growth of chamomile (Matricaria chamomilla L.) and production of essential oil stimulated by arbuscular mycorrhizal symbiosis

This study aimed to evaluate the influence of arbuscular mycorrhizal fungi on the growth and production of capitula and essential oil of chamomile plants, under different growth conditions. The symbiosis between fungi of the phylum Glomeromycota and plant roots may cause changes in secondary metabolism, including the synthesis of essential oils. Increasing biomass production associated with gains in oil content promoted by mycorrhization, makes soil biota management sustainable. Two assays were carried out in a greenhouse, in plastic pots (3 L), for 150 days after emergence of the seedlings. In the first assay, plants were inoculated with soil cultivated with organic chamomile, in three levels (0, 150 and 300 g), with pH adjusted at 6.5, for evaluating the influence of the quantity of inoculum on the growth and production of plants. In the second assay (factorial design with repetition at the central point), three levels of soil pH (6.5, 7.0, 7.5) and three levels of auxin (0; 10; 20 mg L−1) were tested to identify the influence of pH and auxin on the production of chamomile capitula and essential oil. The arbuscular mycorrhizal fungi present as spores in the soil-inoculum were composed of 22 species. In the first assay, mycorrhization favored the growth and production of capitula and such benefits tended to be higher with the increase in concentration of inoculum. In the second assay, the production of capitula and essential oil was promoted by mycorrhization, with the highest content at neutral pH (0.7 mL oil in 30 g of capitula), in synergy with the highest amount of inoculum. It was concluded that the presence of arbuscular mycorrhizal fungi in the soil is the most influential variable in the production of capitula and essential oil of chamomile, but subtle differences on soil pH influenced these parameters. The levels of auxin did not influence significantly plant growth and arbuscular mycorrhizal fungi.

Weed Infestation and Health of Organically Grown Chamomile (Chamomilla recutita (L.) Rausch.) Depending on Selected Foliar Sprays and Row Spacing

Chamomile is a herbal plant of very high economic importance worldwide. Its organically grown raw material is particularly valuable. Under organic farming conditions, weeds and fungal diseases are an important problem in a chamomile plantation. Seeking agronomic solutions designed to eliminate the occurrence of these pathogens in chamomile crops is constantly valid. The aim of the present study was to evaluate the effect of some foliar sprays (enhancing the condition of the crop plant and its competition against pathogens) and different row spacing of two chamomile cultivars on weed infestation and health of a chamomile plantation. The study results presented in this paper were collected from field experiments carried out in the organic system in the village of Dys (the central Lublin region, Poland) over the period 2014–2016. Experiments were conducted on podzolic soil (class III) as a split-block design in 3 replicates in plots with an area of 525 m2 (6.25 m2 a single plot). This study included two chamomile cultivars (“Złoty Łan”, “Mastar”). The second experimental factor was single or double foliar application of three bioproducts (Herbagreen Basic, Bio-algeen, Effective Microorganisms—EM Farming). The other experimental factor was a different row spacing of chamomile (40 cm and 30 cm). The obtained study results show that 10–16 annual weed species and 1–3 perennial species occurred in both chamomile cultivars. Foliar application of the bioproducts contributed to a reduction in the total number of weeds in the crop, but at the same time to greater weed species diversity. In the control treatments (without the bioproducts), the dominance of several weed species (Viola arvensis, Galeopsis tetrahit, Spergula arvensis, Juncus bufonius, Scleranthus annuus) and lower biodiversity of the weed flora were observed. The largest reduction (by about 20%) in the number of annual weeds was found under the influence of the bioproducts Herbagreen Basic and Bio-algeen applied once. Bio-algeen and Effective Microorganisms (EM), in turn, had a significant effect on decreasing the weed weight. A narrower (30 cm) row spacing of chamomile had a significant impact on reducing the weight of weeds in chamomile crops compared to the wider spacing, which was 40 cm. It should be concluded that infection of the chamomile plantation with fungal diseases was overall at a low level. Significantly higher infection with fungal diseases was found in the case of the cultivar “Mastar”, regardless of the experimental factors. A statistically proven decrease in infection of chamomile plants with fungal diseases was determined under lower crop density conditions (a row spacing of 40 cm). Chamomile plants were found to exhibit better health under the influence of double application of the biofertilizers Herbagreen Basic and Bio-algeen.