Results: The percentage share of NFGNB other than PA was 28%. The most common isolated NFGNB were: Stenotrophomonas 10%, Achromobacter 5%, non-aeruginosa Pseudomonas 5%, Sphingomonas 3%, Acinetobacter 2% and others 3%. NFGNB showed variable sensitivity to carbapenems (42–89%), fluoroquinolones (13–100%), aminoglycosides (22–100%), cephalosporins (23–78%), colistin (84–100%), co-trimoxazole (75–97%). Conclusion: We observed significant increase of NFGNB other than PA in colonization of the airways of CF pts. Prolonged incubation and using a selective media increases the frequency of NFGNB isolation. Mass spectrometry is a reliable alternative to conventional biochemical tests used in identification of rarely isolated NFGNB. For some NFGNB species it is required to determine the MIC values to interpret their susceptibility.