T h e specics of Gossypiz~nz havc been categorized by Beasley (1940) into five cytologically and geographically defined diploid genome groups (A, Africa and Asia; B, northern and southern Africa; C, Australia; Dl North and South America; and E, northeastern Africa to Pakistan) and one amphidiploid group (AD, tropical America, Hawaii, and cultivated). Interspecific, intragenomic hybrids gcncrally show cn. 13 bivaleilts at meiotic metaphase I, whereas the uumbcr of bivalints in intergenomic hybrids ranges from less than 1 (for some hybrids involving E genome species) to 11 (A X B hybrids). In 1958 Hutchinson and Lee described a new species of Gossypiz~m from central Tanganyika as G. longicnlyx; collections of this species have also been made in Uganda and southcrn Sudan. They placed the new species in section Stocltsiana (which is coincident with Beasley's E genome group), citing simililrities in thc geographical range and morphology of G. lo~zgicnlyx with species of this scction of the genus. Saunders (1961) later assigned this species the E: subeenome dcsienation. U U Attempts to hybridize G, lo7zgicrrlyx with other Gossypizf~~z taxa have been generally unrewa;ding, but recently a hybrid involving the natural amphidiploid, G. hi.rs.lrtznn, and G. lo7lgicalyx has bcen obtained. T h e present report describcs the cytology of this triploid, and its synthetic hexaploid, which we interpret as evidence that G. longicnlyx represents a new Gossypiz~nz cytotype. Methods and Materials The G. hi7'~~lt~17?~ (and pistillate) parent of the G. hirs~rtz~7?z X lo7zgicnlyx hybrid is accession #820 of the Gossypiz~nz collection of Dr. S. G. Stephens of this institution. Seeds of G. lo~zgicnlyx were reccived through the Cotton and Cordage Fibers Branch, U.S. Department of Agriculture. Three hybrid seeds were obtained: two of the resulting plants vrovided the materials used for the I I study of triploid cytology, the third plant was converted into a hexaploid via the colchicine-tragacanth method of Schwanitz ( 1919). T h e cytological analyses of the two triploid plants were similar, and have been combined in TabIe I. The cytological analyses of PhlC's were made at meiotic metaphase I, following fixation in Carnoy's fluid (4: 3: 1) and storage in 70% ethanol. Results and Discussion The results of the G. hirszrtzr77z X longicrrlyx triploid and hexapIoid cytological analyses are contained in Tablcs I and IT, respectively. A summary of the pertinent literature on the cytology of triploid and hexaploid hybrids combining the AD tetraploids (G. hirszrtunz and G. bnrbacle7zse L.) with species of the A, B, C, D, and E genome groups is presented in Table 111. Comparable data on triploid bivalent frequency and hexaploid multivalent frequency for G. hirszrtzr7n X longicnlyx (6.75 and 2.00, per cell, respectively) and AD X E (0.55 and 0.19, per cell, respectively), indicate that G. lo7zgicalyx