Background During 2016, our Long Term Acute Care Hospital (LTACH) experienced a high rate of Central Line-Associated Bloodstream Infections (CLABSI). This hospital had forty-four CLABSI's, a rate of 4.47 per 1000 central line days. Methods Direct observation by the new Infection Control Practitioner determined many central line dressings to be loose, soiled, and/or in place for longer than 7 days. Further observation confirmed staff knowledge regarding the importance of adhering to aseptic techniques and maintenance of the dressings was deficient. Intensive education of the Nursing staff was initiated in aseptic technique, how to assess the integrity of the central line dressing, weekly and as-needed dressing changes, blood culture collection, and the importance of daily chlorhexidine bathing. Staff was required to be included in the Root Cause Analysis (RCA) on every infection, and the results of each RCA were disseminated at daily Nursing Huddles. Frequent feedback to staff regarding declining rates helped provide a sense of pride and ownership. Results Our CLABSI rate dropped to 1.18 and 2.18 per 1000 central line days in 2017 and 2018, respectively, maintaining a reduction in the CLABSI rate of more than 50%. To verify the decrease, the Hospital-Acquired Infection Prevention Program Liaison from California Department of Public Health performed a validation study in 4th quarter of 2017 and found 100% accuracy in correct determination of CLABSI events, following the National Healthcare Surveillance Network definitions. Conclusions In conclusion, reiteration with emphasis on basic preventative aseptic techniques including the importance of potential infection site observation effectively and dramatically reduced the CLABSI rate at this LTACH. By routinely including the staff in the Root Cause Analysis process, they recognized the importance of these techniques and felt ownership in the results, thereby enhancing ongoing patient care. During 2016, our Long Term Acute Care Hospital (LTACH) experienced a high rate of Central Line-Associated Bloodstream Infections (CLABSI). This hospital had forty-four CLABSI's, a rate of 4.47 per 1000 central line days. Direct observation by the new Infection Control Practitioner determined many central line dressings to be loose, soiled, and/or in place for longer than 7 days. Further observation confirmed staff knowledge regarding the importance of adhering to aseptic techniques and maintenance of the dressings was deficient. Intensive education of the Nursing staff was initiated in aseptic technique, how to assess the integrity of the central line dressing, weekly and as-needed dressing changes, blood culture collection, and the importance of daily chlorhexidine bathing. Staff was required to be included in the Root Cause Analysis (RCA) on every infection, and the results of each RCA were disseminated at daily Nursing Huddles. Frequent feedback to staff regarding declining rates helped provide a sense of pride and ownership. Our CLABSI rate dropped to 1.18 and 2.18 per 1000 central line days in 2017 and 2018, respectively, maintaining a reduction in the CLABSI rate of more than 50%. To verify the decrease, the Hospital-Acquired Infection Prevention Program Liaison from California Department of Public Health performed a validation study in 4th quarter of 2017 and found 100% accuracy in correct determination of CLABSI events, following the National Healthcare Surveillance Network definitions. In conclusion, reiteration with emphasis on basic preventative aseptic techniques including the importance of potential infection site observation effectively and dramatically reduced the CLABSI rate at this LTACH. By routinely including the staff in the Root Cause Analysis process, they recognized the importance of these techniques and felt ownership in the results, thereby enhancing ongoing patient care.