In this paper, a type of vapor chamber with a gradient pore size wick (VC-G) was developed, and its thermal performance was experimentally tested and compared with two types of VCs with uniform pore size wick (which can be defined as VC-U (200) and VC-U (50) as the powder size of the wick is 50-mesh and 200-mesh, respectively) and a VC without a wick (VC-N). In addition, a VC heat transfer ability experiment platform was built, and the thermal resistance, temperature distribution and thermal response time of the VC with different wick structures were experimentally investigated. The experiment results show that the capillary driving force provided by gradient pore size wick increases gradually from outside, which can not only promote the condensation fluid to gather in the central heat source but also facilitate the vapor to spread around. Therefore, compared with VC-U (200), VC-U (50) and VC-N, VC-G shows the best heat transfer performance, temperature uniform performance and start-up performance.