We study the enumeration of off-diagonally symmetric domino tilings of odd-order Aztec diamonds in two directions: (1) with one boundary defect, and (2) with maximally-many zeroes on the diagonal. In the first direction, we prove a symmetry property which states that the numbers of off-diagonally symmetric domino tilings of the Aztec diamond of order 2n−1 are equal when the boundary defect is at the kth position and the (2n−k)th position on the boundary, respectively. This symmetry property proves a special case of a recent conjecture by Behrend, Fischer, and Koutschan.In the second direction, a Pfaffian formula is obtained for the number of “nearly” off-diagonally symmetric domino tilings of odd-order Aztec diamonds, where the entries of the Pfaffian satisfy a simple recurrence relation. The numbers of domino tilings mentioned in the above two directions do not seem to have a simple product formula, but we show that these numbers satisfy simple matrix equations in which the entries of the matrix are given by Delannoy numbers. The proof of these results involves the method of non-intersecting lattice paths and a modification of Stembridge's Pfaffian formula for families of non-intersecting lattice paths. Finally, we propose conjectures concerning the log-concavity and asymptotic behavior of the number of off-diagonally symmetric domino tilings of odd-order Aztec diamonds.