The Aegean domain has experienced lithospheric deformation during continuous northward subduction at the Hellenic trench. Early Cenozoic shortening and thickening were followed by late Cenozoic extension and thinning. Thickened lithosphere, in particular its crust, has excess gravitational potential energy, which is thought to promote orogenic collapse (i.e., gravity spreading). In the Aegean domain, release of this potential energy and collapse of the lithosphere has been proposed as the first-order driver for extension of the overriding plate. Here we show, using dynamically self-consistent analogue experiments of subduction and gravity spreading, that the subducting plate significantly influences overriding plate deformation, despite the overriding plate being positively buoyant. With the introduction of a subducting plate, the trench retreat velocity and overriding plate extension rate increase, which is in contrast with a continuous decline during gravity spreading. In addition, ∼E-W trending normal faults emerge, as opposed to arc-parallel faults trending ∼ENE-WSW to ∼ESE-WNW and arc-normal extensional zones that result from gravity spreading only. Similarly, the subducting plate causes overriding plate displacement to be unidirectional (trenchward), in accordance with the present-day geodetic velocity field, whereas gravity spreading causes a radially outward-directed displacement field. As the decline in trench retreat rate, radial spreading, dominantly arc-parallel normal faults, and arc-normal extensional zones are not observed in nature, this implies that gravity spreading cannot be the sole driver of extensional deformation in the overriding plate. Indeed, the models suggest that subduction-induced mantle flow is the dominant driver of overriding plate deformation because it imposes a basal shear stress on the overriding plate, dragging and extending it trenchward in a unidirectional manner. Our experimental results corroborate earlier studies that highlight the important role of subduction-induced mantle flow in driving overriding plate deformation. Additionally, we find that this role is more pronounced in experiments with a thicker brittle layer.