Cellulose Microspheres Research Articles

In situ growth of ZIF-8 on cellulose microspheres with high doxorubicin loading capacity and pH-sensitive for oral drug delivery

Metal-organic framework (MOF) nanoparticles are a class of porous nanomaterials with wide application prospects. Zeolitic imidazolate framework (ZIF) materials have high loading performance and pH-sensitive characteristics, which means they have great development prospects in the development of drug carrier systems. Herein, cellulose microspheres (CM) were used as a carrier for the nucleation and growth of ZIF-8 nanocrystals. CM@ZIF-8 was prepared in situ, which was more convenient, flexible, cost-effective, and highly safe. CM@ZIF-8, as a novel carrier, was used to monitor the release of the anticancer drug Doxorubicin (DOX) and prevent it from dissipating before reaching its goal. The designed DOX-loaded CM@ZIF-8 (CM@ZIF-8-DOX) system was characterized by FTIR, SEM, N2 sorption isotherm, XRD, and Cytotoxicity assay (cells survival rate 95.08 %). CM@ZIF-8-DOX exhibited controlled drug release behavior in simulated in-vitro tumor microenvironments (81.2 % drug release throughout 72h). CM@ZIF-8 simultaneously had a high loading capacity of DOX (Qe = 159.71 mg∙g−1), and the amount released under acidic conditions (pH 5.0) was greater (63.4 % after 15 h) than under neutral conditions (pH 7.4) due to the detachment of the coordination between metal ions and ligands (37.6 % after 15 h).

Targeted recognition and enhanced biotransformation of phytochemicals by a dual-functional cellulose-based hydrogel bioreactor

The combined technology of molecular imprinting and immobilization is a promising strategy for improving biocatalytic performance. In this work, a dual-functional cellulose-based hydrogel bioreactor with targeted recognition and transformation functions was innovatively designed by cellulose-based molecularly imprinted polymers (CM-MIPs) hydrogel coupled with layered double hydroxide immobilized enzyme (LDH-E). Firstly, CM-MIPs hydrogel was prepared by using phlorizin, 4-pinylpyridine, and cellulose microspheres as template molecule, functional monomer, and support material, respectively. Meanwhile, layered double hydroxide (LDH) taken as a carrier to immobilize β-glucosidase. The prepared LDH was layered sheet-like structure with ultra-thin thickness of approximately only 1.5 nm, and β-glucosidase was immobilized on both sides of the LDH. Further, the dual-functional bioreactor was constructed by the anion exchange, which possessed maximum targeted adsorption capacity to phlorizin of 15.25 mg/g, exhibited 1.2 folds higher transformation efficiency than LDH-E, and the phloretin content increased 26 folds to that in Lithocarpus litseifolius (Hance) Chu leaves extracts. Moreover, the transformation efficiency remained above 70 % even after five consecutive transformations. Overall, the dual-functional bioreactor has broad prospects for the application in targeted recognition and transformation of phytochemicals, and provides a new insight on multifunctional bio-based reactors in natural production field.

Fiber Optics Analysis of Floating Hollow Microspheres for a Poorly Water-Soluble Drug

This study investigates the development and evaluation of floating hollow microspheres for the delivery of omeprazole, a poorly water-soluble drug. The research aims to enhance omeprazole's bioavailability by addressing its low solubility and susceptibility to degradation in an acidic gastric environment. Hollow ethyl cellulose microspheres encapsulating omeprazole were prepared using the solvent evaporation technique. The microspheres displayed 91.3% buoyancy and 82.41% drug entrapment efficiency, with sustained drug release in simulated gastric fluid over 12 hours. Characterization techniques such as X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM) were used. XRD analysis indicated that the encapsulated drug was in an amorphous state, improving dissolution rates and bioavailability. FTIR confirmed successful drug encapsulation, and SEM revealed the morphology and porous structure of the microspheres. The results highlighting the potential of floating hollow microspheres to enhance the oral bioavailability of poorly water-soluble drugs. Recommendations include refining the formulation process and exploring microfluidic technology for generating uniform microspheres with high drug loading. The study provides valuable insights for the development of advanced drug delivery systems.

Macroporous cellulose microspheres with high specific surface area via semi-dissolved chitosan templating for protein separation

The conflict between macroporous structure and high specific surface area strongly limited the dynamic adsorption performance of chromatographic media. Here, macroporous and high-specific-surface-area cellulose microspheres (PCMs) are prepared by using semi-dissolved chitosan as a dual-functional template and subsequently functionalized with diethylaminoethyl (DEAE) as anion exchangers for protein separation. The dual-functional template strategy leverages dissolved chitosan nanofibers as meso/micropore templates and the undissolved chitosan particles as macropore templates. The macropores of DEAE-PCMs (average size: 0.4–1.2 μm) efficiently decrease mass transfer resistance for fast uptake kinetics. The high specific surface area (272.34 m2/g) provides sufficient ligand sites for ensuring exceptional adsorption capacities. Therefore, the chitosan bifunctional templated PCMs, possessing a macro/meso/microporous structure, are promising candidates for high-performance chromatographic media.

Study on the adsorption properties of lysozyme by cellulose microspheres modified with reactive red 120

Microspheres of cellulose acetate, characterized by a multi-layered, uniform, and continuous porous structure, were synthesized through the process of emulsion evaporation. Subsequent deacetylation facilitated the modification of the cellulose microspheres’ surface, which was functionalized with Reactive Red 120, serving as an affinity dye ligand. This modification yielded a cationic adsorbent. The adsorptive behavior of lysozyme from aqueous solutions, with lysozyme designated as the target protein, was examined in relation to the effects of pH and ionic strength. The adsorptive capacity of the cellulose microspheres, modified with Reactive Red 120, for lysozyme was determined to be 106.57 mg g−1, exhibiting rapid equilibration within 40 min. The adsorption kinetics and thermodynamics were accurately described by the pseudo-second-order kinetic model and the Langmuir model, respectively, with correlation coefficients (R2) of 0.98 and 0.97. Furthermore, dynamic adsorption experiments revealed an enhanced adsorption capacity of 36.5 mg g−1, significantly surpassing that of the unmodified microspheres. The cellulose microspheres, derived from cellulose acetate and modified accordingly, are environmentally benign and sustainable, thereby holding significant promise for various biological applications.

Nanofabrication of Losartan Potassium Sustained Release Floating Microspheres using Different Grades of Ethyl Cellulose and its Insight on Release Profiles.

A sustained release system for losartan potassium designed to delay its residence time in the stomach through the preparation of solvent evaporation technique-based floating microspheres. The influence of the different grades of Ethocel™ such as 4 cps, 10 cps, and 22 cps as well as the drug: polymer ratio on various properties of microspheres were tested. Thermal and functional analysis revealed no interaction between the encapsulated drug and polymer. The results indicated that the mean diameter of microspheres increased with a change in grades of ethyl cellulose relating to viscosity. However, the drug incorporation efficiency within ethyl cellulose microspheres decreased with increasing viscosity of ethyl cellulose. The bulk density of the formulations was proportionally dependent on concentration and the viscosity of the polymer, which resulted in a decrease in floating capacity from 90.02% to 73.58%. Moreover, the drug release was indirectly proportional to the viscosity of ethyl cellulose tested. The in vitro release profile exhibited a burst effect with a biphasic release pattern following Fickian diffusion, indicating a diffusioncontrolled release mechanism. The results demonstrated that the viscosity of ethyl cellulose significantly affects the floating capacity and drug release pattern from microspheres.

Synthesis of Cellulose Acetate Butyrate Microspheres as Precursor for Hard Carbon-Based Electrodes in Symmetric Supercapacitors.

Cellulose microspheres have a wide range of applications due to their unique properties and versatility. Various preparation methods have been explored to tailor these microspheres for specific applications. Among these methods, the acetate method using cellulose acetate is well known. However, replacement of the acetate group through the butyrate group significantly extends the variety of morphological properties. In the present work, microspheres based on cellulose acetate butyrate are being developed with modified characteristics in terms of particle size, porosity, surface morphology and the inner structure of the microspheres. While the inner structure of cellulose acetate microspheres is predominantly porous, microspheres prepared from cellulose acetate butyrate are mainly filled or contain several smaller microspheres. Carbon materials from cellulose acetate butyrate microspheres exhibit a high specific surface area of 567 m2 g-1, even without further activation. Activation processes can further increase the specific surface area, accompanied by an adaptation of the pore structure. The prepared carbons show promising results in symmetrical supercapacitors with aqueous 6 M KOH electrolytes. Activated carbons derived from cellulose acetate butyrate microspheres demonstrate an energy density of 12 Wh kg-1 at a power density of 0.9 kW kg-1.

Regulation of macroporous cellulose microspheres via phase separation force induced by carbon nanotubes doping for enhanced protein adsorption

The burgeoning requirement for purified biomacromolecules in biopharmaceutical industry has amplified the exigency for advanced chromatographic separation techniques. Herein, macroporous cellulose microspheres (CCMs) with micron-sized pores are produced by a facile regulation via carbon nanotubes (CNTs). In this strategy, the incorporation of CNTs breaks the homogeneous regeneration of the cellulose, thus providing anisotropic phase force to produce macropores. The CCMs have manifested a faster mass transfer rate and more available adsorption sites owing to well-defined macropores (2.69 ± 0.57 μm) and high specific surface area (147.47 m2 g−1). Further, CCMs are functionalized by quaternary ammonium salts (GTAc-CCMs) and utilized as anion adsorbents to adsorb pancreatic kininogenase (PK). The prepared GTAc-CCMs show rapid adsorption kinetics for PK at pH 6.0, reaching 90 % equilibrium within 60 min. Also, GTAc-CCMs for PK exhibit high adsorptive capacity (632.50 mg g−1), excellent recyclability (> 80 % removal amount after 10 cycles) and selectivity especially at pH 6.0. Notably, the GTAc-CCMs have been successfully applied in a fixed-bed chromatography process, indicating their potential as an effective chromatographic medium for rapid separation of biomacromolecules.

Efficient removal of p-nitrophenol from water by imidazolium ionic liquids functionalized cellulose microsphere

Removing p-nitrophenol (PNP) from water resources is crucial due to its significant threat to the environment and human health. Herein, imidazolium ionic liquids with short/long alkyl chain ([C2VIm]Br and [C8VIm]Br) modified cellulose microspheres (MCC-[C2VIm]Br and MCC-[C8VIm]Br) were synthesized by radiation method. To examine the impact of adsorbent hydrophilicity on adsorption performance, batch and column experiments were conducted for PNP adsorption. The MCC-[C2VIm]Br and MCC-[C8VIm]Br, with an equivalent molar import amount of ionic liquids, exhibited maximum adsorption capacities of 190.84 mg/g and 191.20 mg/g for PNP, respectively, and the adsorption equilibrium was reached within 30 min. Both adsorbents displayed exceptional reusability. Integrating the findings from XPS and FTIR analyses, and AgNO3 identification, the suggested adsorption mechanism posited that the adsorbents engaged with PNP through ion exchange, hydrogen bonds and π-π stacking. Remarkably, the hydrophobic MCC-[C8VIm]Br exhibited superior selectivity for PNP than the hydrophilic MCC-[C2VIm]Br, while had little effect on adsorption capacity and rate. MCC-[C8VIm]Br-2 with high grafting yield increased the adsorption capacity to 327.87 mg/g. Moreover, MCC-[C8VIm]Br-2 demonstrated efficient PNP removal from various real water samples, and column experiments illustrated its selective capture of PNP from groundwater. The promising adsorption performance indicates that MCC-[C8VIm]Br-2 holds potential for PNP removal from wastewater.

Peptides-modified cellulose microspheres for adsorption of ochratoxin A: Performance and mechanism

In this study, peptides with ochratoxin A (OTA) recognition and adsorption capability were designed, among which lysine, histidine, arginine and tyrosine were crucial. Peptide CYFRFYSNLHPK showed the strongest affinity for OTA (Ka = 1.74 × 105 M−1). Theoretical calculations revealed that the differences in the affinity of peptides for OTA were attributed to differences in the type, number and position of amino acids residues. Peptides were conjugated with porous cellulose microspheres to form peptide-modified pretreatment materials (Pep-MPA-RCMs) for adsorption of OTA. The morphology and composition of the prepared Pep-MPA-RCMs were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, fourier transform infrared spectroscopy, thermogravimetric analysis and X-ray diffraction. The effects of pH, adsorption time, initial concentration and temperature on the adsorption process were investigated. The adsorption performance was correlated with the pseudo-second order kinetic and Sips adsorption isotherm model. Thermodynamic studies further proved that the adsorption was exothermic and the prepared Pep-MPA-RCMs showed the maximum adsorption capacity of 9.87 mg/g for OTA at 25℃. A simple adsorption column for OTA was prepared by using Pep-MPA-RCMs as filler material, which still maintained more than 80 % of the removal rate after five consecutive adsorption-elution cycles. The prepared pretreatment material was successfully applied to remove OTA in real samples and was a promising adsorbent for practical applications.

Carbonized cellulose microspheres loaded with Pd NPs as catalyst in p-nitrophenol reduction and Suzuki-Miyaura coupling reaction

Catalytic reduction of p-nitrophenol is usually carried out using transition metal nanoparticles such as gold, palladium, silver, and copper, especially palladium nanoparticles (Pd NPs), which are characterized by fast reaction rate, high turnover frequency, good selectivity, and high yield. However, the aggregation and precipitation of the metals lead to the decomposition of the catalyst, which results in a significant reduction of the catalytic activity. Therefore, the preparation of homogeneous stabilized palladium nanoparticles catalysts has been widely studied. Stabilized palladium nanoparticles mainly use synthetic polymers. Cellulose microspheres, as a natural polymer material with low-cost and porous fiber network structure, are excellent carriers for stabilizing metal nanoparticles. Cellulose microspheres impregnated with palladium metal nanoparticles were carbonized to have a larger specific surface area and highly dispersed palladium nanoparticles, which exhibited excellent catalytic activity in the catalytic reduction of p-nitrophenol. In this work, the cellulose carbon-based microspheres palladium (Pd@CCM) catalysts were designed and characterized by SEM, TEM, EDS, XRD, FTIR, XPS, TGA, BET, and so on. Furthermore, the catalytic performance of Pd@CCM catalysts was investigated via p-nitrophenol reduction, which showed high catalytic activity. This catalyst also exhibited excellent catalytic performance in the Suzuki-Miyaura coupling reaction. Linking aromatic monomer and benzene through Suzuki-Miyaura coupling was presented as an effective route to obtaining biaryls, and the synthesis method is low-cost and simple. In addition, Pd@CCM showed desirable recyclability while maintaining its catalytic activity even after five recycles. This work is highly suggestive of the design and application of the heterogeneous catalyst.

Fusion of cellulose microspheres with pulp fibers: Creating an unconventional type of paper

Cellulose microspheres (CMS) are a type of spherical regenerated cellulose particles with versatile properties which have been used as carrier materials in medical and technical applications. The integration of CMS into paper products opens up novel application scenarios for paper products in a wide range of fields. However, the incorporation of CMS carriers into paper products is challenging and hitherto no reports do exist in literature. Here, we present a feasibility study to incorporate up to 50 w.% CMS in paper hand sheets using retention aids. Our primary observations highlight the successful formation of uniform paper hand sheets retaining its tensile strengths at elevated CMS concentrations. Sheets with high CMS contents exhibit an increase in density and display enhanced surface smoothness — an outcome of a CMS layer forming atop the fiber base — which effectively bridges voids and rectifies surface irregularities as supported by Gurley testing, infinite focus microscopy and scanning electron microscopy. While our primary objective centered on the general feasibility to manufacture CMS-containing papers, the resulting composite scaffold carries significant potential as a platform for innovative, functional paper-based materials.

Formulation and evaluation of cidofovir loaded microspheres

The study aimed to produce and assess cidofovir microspheres using the emulsion solvent diffusion method. Microspheres loaded with ethyl cellulose and HPMC were prepared. FTIR analysis confirmed stability with no drug-polymer interaction, and compatibility investigations ensured formulation compatibility. SEM determined particle size, with yields ranging from 84.44% to 87.62% and drug content from 64.8% to 97.2%. Particle sizes ranged from 20 µm to 210 µm, with drug loading capacities of 55.6% to 96.8% and entrapment efficiencies of 55.5% to 89.1%. Swellability studies showed a range of 0.6 to 1.6 seconds. Formulation CM8 exhibited optimal dissolution in vitro at 62.87%. Drug dissolution data were fitted to various models, with R2 values ranging from 0.937 to 0.061. Up to 45 minutes of drug release was observed. Overall, cidofovir-loaded HPMC and ethyl cellulose microspheres showed favorable release properties under optimal conditions.

Synthesis of dioctyl sebacate catalysed by cellulose-immobilized lipase

Dioctyl sebacate is a long carbon chain dibasic acid ester with methylene as the main body. It is widely used in plasticizers because of its good cold resistance and high viscosity. And it has excellent properties of non-toxicity and easy biodegradation, so it can be used as a potential new green bio-based plasticizer. At present, most of the preparation methods of dioctyl sebacate are chemical methods, which have many by-products and cause environmental pollution. In this article, cellulose microspheres were used as a carrier to immobilize Candida antarctica lipase B (CALB) to catalyse the esterification of sebacic acid and n-octanol to prepare dioctyl sebacate. Through single factor experiments, the lipase immobilized on cellulose microspheres was optimized to determine the best process conditions: add 500 µL CALB to the erlenmeyer flask, and then add 0.02 mol/L PBS buffer (pH 8.0). After being prepared into a 5 mL system, 0.1 g of cellulose microspheres were added as a carrier and reacted for 3 h at 35 °C and 180 r/min. Added 0.5 vt% polyethylene glycol diglycidyl ether (PEGDEG) to the system for crosslinking. The final immobilization rate of lipase immobilized on cellulose microspheres was 80.06%, and the enzyme activity was 288.07 U/g. Using the cellulose-immobilized lipase as a catalyst, in the toluene system, the molar ratio of n (sebacic acid)/n (n-octanol) substrate was 1:3.5, the amount of immobilized enzyme was 0.04 g, 4 Å molecular sieve 1.5 g, 40 °C, and 150 r/min for 30 h, the conversion rate of dioctyl sebacate was 76.45%.

A sphere-on-sphere structure: Cu nanoparticles grown on cellulose microspheres for the removal of environmental contaminants

Herein, sphere-on-sphere (SOS) structural Cu@porous cellulose microspheres (SOS Cu@PCMs) are successfully synthesized by a space-confined reduction strategy with nano-sized Cu particles grown on PCMs. Typically, the customized PCMs matrix is epoxically modified by epichlorohydrin (EPI) and subsequently grafted by glycine, endowing PCMs with an ability to chelate Cu2+. After subsequent reduction reaction by N2H4·H2O, an innovative SOS structural Cu@PCMs are successfully acquired (specific surface area: 230.68 m2 g−1, average diameter: 41.00 μm and average pore diameter: 39.78 nm). The SOS structure not only protects hierarchical porous structure to enhance mass transfer but also facilitates industrial operability and recyclability of Cu. Benefit from the SOS structure, Cu@PCMs perform exceptionally well in facilitating the degradation of water contaminants, such as 4-nitrophenol (apparent rate constant of −0.009 s−1, removal within 240 s) and azo dyes (degradation within 240 s for methyl orange, methylene blue and congo red). Above all, the SOS structural Cu@PCMs present a highly promising candidate in the field of wastewater remediation.

Facile and Scalable Fabrication of Regenerated Cellulose Microspheres with High Strength and Porosity as a Potential Matrix for Hemoperfusion

For acquiring an ideal sorbent for hemoperfusion, three crucial problems should be addressed: 1. The gap between the stacked sorbents should be larger than the blood cells, around 600 μm, for a continuous pass-through of blood in a hemoperfusion device. 2. The hemoperfusion device should maintain at least a 190 ml/min flow rate. 3. Specific binding on the sorbents is required to absorb the harmful agent in blood. In this research we developed a low-surfactant emulsification technique to prepare physically crosslinked cellulose microspheres (CMs). The particle size of the CMs could be tuned from 150 μm to 1000 μm. Three dehydration methods also regulated the mechanical properties of the CMs. The perfusion column stacked by physically crosslinked CMs, after being treated by an ethanol bath, could withstand pressure induced by a flow rate of 190 ml/min. The adsorption processes of hydrophobic alkyl carbon chain (C18) modified CMs for bilirubin conformed to the Langmuir isothermal adsorption model, which indicated that the bilirubin adsorption by C18 grafted CMs mainly relied on the interaction between C18 and bilirubin. In summary, a facile, scalable and low-surfactant fabrication strategy was developed to synthesize regenerated cellulose microspheres with high strength and porosity as a promising absorbent for hemoperfusion.

Restricted reaction of layered double hydroxide nanoparticles with phosphate in a confined microsphere space

Over the past decades, considerable efforts have been made to find useful solutions for phosphate pollution control. The state transition of nanomaterials from freely dispersed to encapsulated provides a realizable route for their application in phosphate elimination. The separation convenience offered by encapsulation has been widely recognized, however, the unique binding mode of nanostructures and phosphate in the confined space remains unclear, limiting its further development. Here, carboxymethyl cellulose (CMC) microspheres were used as hosts to deploy layered double hydroxide (LDH) nanoparticles. On this basis, we described an attempt to explore the adsorption behavior of LDH and phosphate in the microsphere space. Compared to their freely dispersed analogues, LDH particles exhibited higher structural stability, wider pH adaptability, and better phosphate selectivity when spatially confined in the CMC microsphere. Nevertheless, the kinetic process was severely inhibited by three orders of magnitude. Besides, the saturated phosphate adsorption capacity was also reduced to 74.6 % of the freely dispersed system. A combinative characterization revealed that the highly electronegative CMC host not only causes electrostatic repulsion to phosphate, but also extracts the electron density of the metal center of LDH, weakening its ability to act as a Lewis acid site for phosphate binding. Meanwhile, the microsphere encapsulation also hinders the ion exchange function of interlayer anions and phosphate. This study offers an objective insight into the reaction of LDH and phosphate in the confined microsphere space, which may contribute to the advanced design of encapsulation strategies for nanoparticles.

“Green” electrostatic droplet-assisted forming cellulose microspheres with excellent structural controllability and stability for efficient Cr(VI) removal

This study presents a novel and environmentally friendly method for producing cellulose microspheres (CM) with controllable morphology and size using electrostatic droplets. The traditional droplet method for CM production requires complex equipment and harmful reagents. In contrast, the proposed method offers a simple electrostatic droplet approach to fabricate CM10 at 10 kV, which exhibited a smaller volume, linear microscopic morphology, and a larger specific surface area, with a 36.60 % improvement compared to CM0 (prepared at 0 kV). CM10 also demonstrated excellent underwater structural stability, recovering in just 0.5 s, and exhibited the highest adsorption capacity for Cr(VI) at 190.16 mg/g, a 72.15 % improvement over CM0. This enhanced adsorption capacity can be attributed to the unique structure of CM10 and the introduction of more amino groups. Moreover, CM10 displayed good cyclic adsorption capacity and high dynamic adsorption efficiency, making it highly suitable for practical applications. CM10 exhibited remarkable adsorption capacity, stability, and practical value in treating Cr(VI) wastewater. This work proposes a simple and eco-friendly method for producing CM with excellent structural controllability and stability, providing an effective route for wastewater treatment.

Efficient and selective adsorption of TcO4−/ReO4− by n-alkyl-imidazolium ionic liquids functionalized cellulose microspheres and their application in simulated Beishan groundwater

Removal of technetium from radioactive waste has become attractive due to its huge environmental hazard. Herein, a series of n-alkyl-imidazolium ionic liquids functionalized cellulose microspheres (MCC-[CnVIm]Br, n = 2, 4, 6, 8, 10) were synthesized by radiation method, and their grafting regularity were investigated. In order to explore the effect of alkyl chain length on adsorption performance, the adsorption behaviors of MCC-[CnVIm]Br for TcO4−/ ReO4− were researched in detail via batch and column experiments. MCC-[CnVIm]Br exhibited high ReO4− adsorption efficiency over a wide pH range from 4 to 9, and the maximum adsorption capacity was 230.41 mg/g. The adsorption efficiency of MCC-[CnVIm]Br barely changed after 5 times reused. Remarkably, the selectivity of ReO4− enhanced with the increase of alkyl chain length, while the adsorption capacity and rate were hardly affected by it. The column experiment showed that MCC-[C10VIm]Br can selectively capture ReO4− from simulated Beishan groundwater. The adsorption performance suggested that MCC-[C10VIm]Br is promising for high-efficiency uptake of ReO4− from Beishan groundwater and other radioactive wastes.

Supramolecular cellulose-based heavy metal adsorbent for efficient and accurate removal of cobalt (II) for water treatment

Heavy metal ions' high toxicity, carcinogenicity, and non-degradability have been identified as the most severe environmental concerns. Therefore, in this study, a new type of cellulose-based ionic imprinting adsorbent (p-CMs@EPI@PEI@Cys-IIP) with a three-dimensional pore structure was developed to selectively adsorb cobalt (II) using a simple ion-cross-linking technique. In this experiment, p-CMs@EPI@PEI@Cys-IIP were prepared by modifying cellulose microspheres (p-CMs) with epichlorohydrin (EPI), polyethyleneimine (PEI) and L-cysteine (L-Cys).The p-CMs@EPI@PEI@Cys-IIP was found to be able to selectively adsorb Co (II), and it was well characterized with FT-IR, SEM, TGA, and XRD. The system adsorption experiment proved that the maximum adsorption capacity reached 158.93 mg g-1, below the pH 7. After five cycles, the adsorption capacity of p-CMs@EPI@PEI@Cys-IIP still remained above 90%. The results of selectivity experiments indicated that p-CMs@EPI@PEI@Cys-IIP has preferential selectivity for Co (II). The adsorption isotherm and kinetic experiment of Co (II) conformed to the Langmuir model and pseudo-second-order kinetic, respectively. The adsorption process of Co (II) on p-CMs@EPI@PEI@Cys-IIP was also affected by the intra-particle diffusion model. Due to its simple preparation method and good adsorption performance, p-CMs@EPI@PEI@Cys-IIP would have a good application prospect in the selective removal of Co (II) for water treatment.