Cells In Stool Research Articles

Clinical Assessment and Demographic Insights of Lactose Intolerance Among Diarrheal Children at Hyderabad, Pakistan

Lactose intolerance is common among diarrheal children due to compromised gut health. Diarrhea can damage the intestinal lining, reducing lactase enzyme production responsible for lactose digestion. Consequently, lactose, a sugar found in dairy products, remains undigested, leading to abdominal discomfort, bloating, and increased bowel movements. Objective: To assess the clinical characteristics of Lactose intolerance (LI) as well as its relationship with demographic factors among diarrheal children below five years of age. Methods: A cross sectional study was conducted enrolling 50 diarrheal patients in equal proportion by gender. The present study was conducted over children suffering from profuse diarrhea admitted to the Pediatrics ward at LUMHS and CIVIL hospital Hyderabad, Pakistan during July 2018 to January 2019. The questionnaire-based analysis was conducted to gather information regarding dietary index and manifestation of symptoms after milk consumption. Clinical analysis was performed using lactose tolerance test, Stool pH and reducing substance respectively. The obtained results were analyzed using SPSS. Results: 20 children were found to be suffering from lactose intolerance. The clinical symptoms observed among individuals affected by LI included loose motion, weight loss, abdominal distention, and the presence of pus cells in stool indicating the signs of infection. T-test showed statistical significance (p-value ˂ 0.05) over physical attributes such as height and number of pus cells among LI patients as compared to lactose tolerant (LT) patients. The finding of pus cells in the stool simultaneous to the strong statistical correlation between relieve in symptoms with increasing age also affirmed the existence of secondary type hypo-lactasia. The study also highlighted the demographic aspects contributing to the prevalence of the condition. Conclusions: Secondary lactose intolerance was found with shortened heights of patients and increased number of pus cells in stool.

Case Report: Stemi With Covid-19

BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV‑2) causing coronavirus disease 2019 (COVID-19) has reached pandemic levels by March 2020. Patients with cardiovascular disease, particularly with cardiac injury represent a vulnerable population and increased risk of mortality and morbidity. There is still no guidelines for management of cardiovascular disease during the COVID-19 pandemic. CASE ILLUSTRATION An unconscious 52-year-old male brought to ER with complaints of abdominal discomfort and nausea. The patient had a cardiac arrest in ER and ROSC was obtained. The patient was intubated ECG showed anterior STEMI and primary PCI was performed. The endotracheal tube was changed due to blockage of excessive and thick slime. Tracheostomy was performed. Bronchoscopy was performed and found tracheal mucosal edema, hyperemic and easily bleed; mucous plug and blood clots in the tip of tracheostomy cannula. Thoracic CT-Scan showed ground-glass appearance and fibrosis of the 6th thoracic dextra segment. PCR SARS-CoV-2 showed reactive. The patient was discharged from our hospital after three weeks with clinically stable and referred to COVID-19 center hospital nearby his home for another two weeks. After PCR SARS CoV-2 was performed twice showed negative results, the patient was discharged. CONCLUSION SARS-CoV‑2 infection may lead to acute myocardial injury through viral systemic inflammation, although specific mechanism remained uncertain. A thick mucus plaque and stool cell may be a specific clinical features in COVID-19 patients. Tracheostomy has a continuing role in managing weaning from extended periods of mechanical ventilation during the COVID-19 pandemic.

Exfoliated epithelial cell transcriptome reflects both small and large intestinal cell signatures in piglets.

Assessing intestinal development and host-microbe interactions in healthy human infants requires noninvasive approaches. We have shown that the transcriptome of exfoliated epithelial cells in feces can differentiate breast-fed and formula-fed infants and term and preterm infants. However, it is not fully understood which regions of the intestine that the exfoliated cells represent. Herein, the transcriptional profiles of exfoliated cells with that of the ileal and colonic mucosa were compared. We hypothesized that exfoliated cells in the distal colon would reflect mucosal signatures of more proximal regions of the gut. Two-day-old piglets (n = 8) were fed formulas for 20 days. Luminal contents and mucosa were collected from ileum (IL), ascending colon (AC), and descending (DC) colon, and mRNA was extracted and sequenced. On average, ∼13,000 genes were mapped in mucosal tissues and ∼10,000 in luminal contents. The intersection of detected genes between three mucosa regions and DC exfoliome indicated an approximately 99% overlap. On average, 49% of the genes in IL, AC, and DC mucosa were present in the AC and DC exfoliome. Genes expressed predominantly in specific anatomic sites (stomach, pancreas, small intestine, colon) were detectable in exfoliated cells. In addition, gene markers for all intestinal epithelial cell types were expressed in the exfoliome representing a diverse array of cell types arising from both the small and large intestine. Genes were mapped to nutrient absorption and transport and immune function. Thus, the exfoliome represents a robust reservoir of information in which to assess intestinal development and responses to dietary interventions.NEW & NOTEWORTHY The transcriptome of exfoliated epithelial cells in stool contain gene signatures from both small and large intestinal mucosa affording a noninvasive approach to assess gut health and function.

The non-invasive exfoliated transcriptome (exfoliome) reflects the tissue-level transcriptome in a mouse model of NSAID enteropathy

Non-steroidal anti-inflammatory drugs (NSAIDs) are among the most frequently used classes of medications in the world, yet they induce an enteropathy that is associated with high morbidity and mortality. A major limitation to better understanding the pathophysiology and diagnosis of this enteropathy is the difficulty of obtaining information about the primary site of injury, namely the distal small intestine. We investigated the utility of using mRNA from exfoliated cells in stool as a means to surveil the distal small intestine in a murine model of NSAID enteropathy. Specifically, we performed RNA-Seq on exfoliated cells found in feces and compared these data to RNA-Seq from both the small intestinal mucosa and colonic mucosa of healthy control mice or those exhibiting NSAID-induced enteropathy. Global gene expression analysis, data intersection, pathway analysis, and computational approaches including linear discriminant analysis (LDA) and sparse canonical correlation analysis (CCA) were used to assess the inter-relatedness of tissue (invasive) and stool (noninvasive) datasets. These analyses revealed that the exfoliated cell transcriptome closely mirrored the transcriptome of the small intestinal mucosa. Thus, the exfoliome may serve as a non-invasive means of detecting and monitoring NSAID enteropathy (and possibly other gastrointestinal mucosal inflammatory diseases).

Computerized detection of leukocytes in microscopic leukorrhea images.

Detection of leukocytes is critical for the routine leukorrhea exam, which is widely used in gynecological examinations. An elevated vaginal leukocyte count in women with bacterial vaginosis is a strong predictor of vaginal or cervical infections. In the routine leukorrhea exam, the counting of leukocytes is primarily performed by manual techniques. However, the viewing and counting of leukocytes from multiple high-power viewing fields on a glass slide under a microscope leads to subjectivity, low efficiency, and low accuracy. To date, many biological cells in stool, blood, and breast cancer have been studied to realize computerized detection; however, the detection of leukocytes in microscopic leukorrhea images has not been studied. Thus, there is an increasing need for computerized detection of leukocytes. There are two key processes in the computerized detection of leukocytes in digital image processing. One is segmentation; the other is intelligent classification. In this paper, we propose a combined ensemble to detect leukocytes in the microscopic leukorrhea image. After image segmentation and selecting likely leukocyte subimages, we obtain the leukocyte candidates. Then, for intelligent classification, we adopt two methods: feature extraction and classification by a support vector machine (SVM); applying a modified convolutional neural network (CNN) to the larger subimages. If different methods classify a candidate in the same category, the process is finished. If not, the outputs of the methods are provided to a classifier to further classify the candidate. After acquiring leukocyte candidates, we attempted three methods to perform classification. The first approach using features and SVM achieved 88% sensitivity, 97% specificity, and 92.5% accuracy. The second method using CNN achieved 95% sensitivity, 84% specificity, and 89.5% accuracy. Then, in the combination approach, we achieved 92% sensitivity, 95% specificity, and 93.5% accuracy. Finally, the images with marked and counted leukocytes were obtained. A novel computerized detection system was developed for automated detection of leukocytes in microscopic images. Different methods resulted in comparable overall qualities by enabling computerized detection of leukocytes. The proposed approach further improved the performance. This preliminary study proves the feasibility of computerized detection of leukocytes in clinical use.

Epidemiology of Adenovirus and Genotypic Characteristics of Group A Rotavirus Among Diarrhoeic Children Aged 0 to 5 Years Old in the Ngaoundere Region of Cameroon

In the context of recent introduction of a Rotavirus vaccine, little is known about the epidemiology of Rotavirus and Adenovirus gastroenteritis in Cameroon in general and in Ngaoundere specifically. The main objective of this study was to determine the frequency of Rotavirus and Adenovirus antigens as well as to characterise circulating Rotavirus strains in children with diarrhoea in Ngaoundere. A convenience sampling method was used and stool samples collected from patients consulting at the study sites from April to July 2014 were subjected to a qualitative immunochromatographic test to detect group antigens of group A Rotavirus and Adenovirus. Socio-demographic data were collected using a pre-tested questionnaire. Stool samples tested positive for Group A Rotavirus were genotyped by multiplex nested RT-PCR method. Logistic regression, Chi-square or Fisher’s exact tests were performed when appropriate. P-value < 0.05 at 95% confidence interval was considered statistically significant. Adenovirus antigen was detected in 26.4% (56) of children, whereas, group A Rotavirus was detected in 2.3% (5); the co-infection rate was 5.3%. Vomiting, fever, moderate dehydration, severe dehydration and the presence of polymorphonuclear cells in stool were significantly associated with the presence of Adenovirus antigen in stool. Group A Rotavirus was also found to circulate in Ngaoundere and the G1P[8] genotype showed indication of being the most represented. Adenovirus antigen was shown to be an important agent in childhood acute gastroenteritis in Ngaoundere and this study has been used as a baseline data for this infection in this region of Cameroon. The appropriate management of viral acute gastroenteritis necessitates the detection of Rotavirus and Adenovirus in clinical routine practice. Furthermore, Rotarix™ vaccination should be encouraged and the populations should be educated on hygiene practices.

CLINICAL AND PARACLINICAL CHARACTERISTITICS OF ROTAVIRUS DIARRHEA IN CHILDREN LESS THAN 5 YEARS OLD IN PEDIATRIC DEPARTMENT OF BINH DINH GENERAL HOSPITAL

Background: Diarrhea is a leading cause of morbidity and mortality in children in developing countries. Rotavirus is the most important factor cause of severe diarrhea and mortality in children, especially under 2 years. Objective: To study Clinical and paraclinical characteristitics of rotavirus diarrhea in children less than 5 years old in the Pediatric Department of Binh Dinh General Hospital. Methods: Cross observational study, 417 children from 2 months to 5 years old who were hospitalized for acute diarrhea without blood in stool in Pediatric Department of Binh Dinh general hospital, from 15/3/2012 to 14/3/2013. There were 228 patients with rotavirus - positive stool specimens and 189 patients with rotavirus - negative stool specimens. Tested for rotavirus A classification by agglutination techniques. Results: Percentage of Rotavirus diarrhea in children &lt;5 years was 54.7%. The mean age of rotavirus diarrhea was 14.83 ± 9.08 tháng months. Ages with the highest cases of rotavirus diarrhea are 2 - 12 months: 48.2%. Rotavirrus diarrhea is experienced in every month but, the highest prevalence is experienced in the spring - summer) with 71,5%. Clinical characteristitics of rotavirus diarrhea: Vomit presents in 91,7% of cases. 32.9% of the initial symptoms of the disease is vomiting. Mean number of vomiting episodes per day: 7 ± 3.57 times, significantly higher compared with non-rotavirus acute diarrhea (p &lt;0,01). Mean number of bowel movement per day: 10.61±4.18 times, mean duration of diarrhea: 6.66 ± 2.52 days. Younger children have higher frequency and duration of diarrhea compared to older children. Paraclinical characteristitics of rotavirus diarrhea: the mean number leukocyte: 9.74 ±3.61 (x 109/l). 2.2% with low blood sodium status; 8.8% with reduction in serum potassium concentration. 26.3% with low blood calcium and glucose status. Laboratory characteristics of stool: 8.8% with white blood cells in stool, 41.7% with carbohydrate in stool. Conclusion: Rotavirus accounting for 54.7% of the causes of diarrhea in children. Rotavirus diarrhea is experienced throughout the year but the highest prevalence is in the spring-summer. Prominent symptoms of the disease are vomiting and watery stool. Children &lt;24 months experience higher frequency and duration of diarrhea compared to older age groups. Key words: Diarrhea, Rotavirus.

Non-typhoidal Salmonella gastroenteritis at a diarrheal hospital in Dhaka, Bangladesh, 1996-2011.

Data on non-typhoidal Salmonella (NTS) infection in South Asia are limited. We used data gathered prospectively from 1996 to 2011 as part of a hospital surveillance system in Dhaka, Bangladesh, to identify diarrheal patients with NTS isolated from stool. NTS was isolated in 1.3% (468 of 37,439) of diarrheal patients; 47% of total cases of NTS were in children < 5 years of age, although older adults (≥ 60 years) had the highest isolation rates. NTS isolation peaked in the monsoon months of July and August. Over the study period, rates of multidrug resistance decreased, whereas rates of decreased susceptibility to ciprofloxacin increased. Compared with control patients, NTS patients were older and wealthier; however, no differences in type of housing or exposure to animals were found. NTS patients had increased inflammatory cells in stool and required more fluid resuscitation.

Exfoliated Cells in Stool: A Source for Reverse Transcription-PCR–Based Analysis of Biomarkers of Gastrointestinal Cancer

Because the colonic mucosa is in direct contact with digesta, luminal exposure to potentially carcinogenic or chemopreventive agents may be important in colorectal carcinogenesis, independently of the effects of systemic exposure through the circulation. To date, few biomarkers for luminal exposure have been identified, and isolation of reasonably good quality fecal human RNA remains difficult. In this study, we assessed the yield and quality of RNA extracted from 10 human stool samples after storage with several commercially available preservatives compared with stool samples immediately frozen in liquid nitrogen. This study shows that careful design of primer pairs which amplify a short length of DNA is key to obtaining interpretable and reproducible results. Moreover, the use of commercially available RNA preservation kits enables investigators to collect usable fecal samples from large populations. Of all the preservative methods tested, RNAlater had the best performance in terms of overall quality, quantity, and level of genomic DNA contamination, and thus deserves further investigation.

Extraction of colorectal exfoliative cells in stool

Objective To improve the approaches of isolating exfoliated cells from faece,further to analyze the role of exfoliated cells in diagnosis of colorectal cancer. Methods Thirty\|one patients with colorectal cancer were entered in our study. Exfoliated cells in the stool of patients were isolated by both methods of elutriation and Immonumagnetic beads (IMB). The method of IMB was performed according to Loktionov , s method. and Elutriation means was Iyengar , s method with slight modification. Results The exfoliative cytology sensitivity for colorectal cancer by elutriation was significant higher than that by IMB( P 0.01).Conclusions The improved method of isolation exfoliated cells from stool has advantage of easy to operate and low cost,so it applied to be spread.Detection of exfoliated cells in stool plays an important role in diagnosis of colorectal cancer.

MEMORANDUM FOR: Science Writers and Editors on the Journal Press List: Detecting Colon Cancer Through DNA Testing of Cells in Stool

MEMORANDUM FOR: Science Writers and Editors on the Journal Press List: Detecting Colon Cancer Through DNA Testing of Cells in Stool