Cell Temperature Monitoring Research Articles

A Lanthanide Upconversion Nanothermometer for Precise Temperature Mapping on Immune Cell Membrane.

Cell temperature monitoring is of great importance to uncover temperature-dependent intracellular events and regulate cellular functions. However, it remains a great challenge to precisely probe the localized temperature status in living cells. Herein, we report a strategy for in situ temperature mapping on an immune cell membrane for the first time, which was achieved by using the lanthanide-doped upconversion nanoparticles. The nanothermometer was designed to label the cell membrane by combining metabolic labeling and click chemistry and can leverage ratiometric upconversion luminescence signals to in situ sensitively monitor temperature variation (1.4% K-1). Moreover, a purpose-built upconversion hyperspectral microscope was utilized to synchronously map temperature changes on T cell membrane and visualize intracellular Ca2+ influx. This strategy was able to identify a suitable temperature status for facilitating thermally stimulated calcium influx in T cells, thus enabling high-efficiency activation of immune cells. Such findings might advance understandings on thermally dependent biological processes and their regulation methodology.

Hardware/Software Data Acquisition System for Real Time Cell Temperature Monitoring in Air-Cooled Polymer Electrolyte Fuel Cells

This work presents a hardware/software data acquisition system developed for monitoring the temperature in real time of the cells in Air-Cooled Polymer Electrolyte Fuel Cells (AC-PEFC). These fuel cells are of great interest because they can carry out, in a single operation, the processes of oxidation and refrigeration. This allows reduction of weight, volume, cost and complexity of the control system in the AC-PEFC. In this type of PEFC (and in general in any PEFC), the reliable monitoring of temperature along the entire surface of the stack is fundamental, since a suitable temperature and a regular distribution thereof, are key for a better performance of the stack and a longer lifetime under the best operating conditions. The developed data acquisition (DAQ) system can perform non-intrusive temperature measurements of each individual cell of an AC-PEFC stack of any power (from watts to kilowatts). The stack power is related to the temperature gradient; i.e., a higher power corresponds to a higher stack surface, and consequently higher temperature difference between the coldest and the hottest point. The developed DAQ system has been implemented with the low-cost open-source platform Arduino, and it is completed with a modular virtual instrument that has been developed using NI LabVIEW. Temperature vs time evolution of all the cells of an AC-PEFC both together and individually can be registered and supervised. The paper explains comprehensively the developed DAQ system together with experimental results that demonstrate the suitability of the system.

Model-based On-board Monitoring for Lithium-Ion Batteries

Abstract Monitoring of lithium-ion batteries is a challenging task due to the nonlinear behavior, especially on-board a vehicle. In this article, a model-based monitoring method for an automotive battery pack is presented based on a linear parameter-varying model. The model is obtained by system identification using cell measurement data and extended to the battery pack. It is shown how such a model can be used either for cell temperature monitoring or for monitoring of the state of health of the battery. Results are demonstrated using driving cycle measurements from a battery pack for hybrid vehicles.