Cedarwood Essential Oil Research Articles

Synergistic Antioxidant Activity of Four—Component Mixture of Essential Oils: Basil, Cedarwood, Citronella and Thyme for the Use as Medicinal and Food Ingredient

Mixture design is a statistical tool used to obtain the maximum desired effect using the minimum number of experiments. The aim of the presented work was the optimization of the composition of a mixture of essential oils from basil, citronella, cedarwood and thyme using simplex-lattice mixture design method. The optimized parameter was an antioxidant activity measured in DPPH assay and expressed as effective concentration (EC50). The test results showed an interesting synergy between the components of essential oils. The prepared binary and quaternary mixtures were characterized by higher activity than simple average activity. The designed mixture with approximated highest antioxidant activity was composed of: 54.4% citronella essential oil, 33.0% thyme essential oil, 9.2% cedarwood essential oil and 3.4% basil essential oil and its approximated activity was in agreement with experimental values. This work confirmed that it is possible to approximate the best antioxidant composition of four essential oils used as a potential medicinal and food ingredient.

Cedrus atlantica (Endl.) Manetti ex Carrière Essential Oil Alleviates Pain and Inflammation with No Toxicity in Rodent

Cedrus atlantica (Endl.) Manetti ex Carrière is an endemic tree with spiritual value, and it was used since immemorial time in folk medicine. The present study aims to evaluate the anti-inflammatory (carrageenan-induced paw edema and formalin tests) and analgesic effects (hot plate and acetic acid writhing tests) of the cedarwood essential oil, as well as inspect any toxicity (acute toxicity), using several in vivo assays. Following the acetic acid writhing test and the hot plate test, the EO presented an excellent analgesic effect compared to the controls, especially with the dose of 50 mg/kg. Similar results were found while assessing the anti-inflammatory potential in the carrageenan-induced paw edema and formalin assays. The acute toxicity assessment and the subsequent monitoring of the animals, the biochemical analysis, and the relative organ weight, demonstrated a total safety of the EO. The GC/MS analysis of the composition revealed that the major compounds contained in this EO are beta-himachalene (51.95%), followed by alpha-himachalene (15.82%), and gamma-himachalene (12.15%). This study supports the usage of this tree EO to alleviate pain and inflammation.

Fate-directed risk assessment of chemical mixtures: a case study for cedarwood essential oil.

The environmental risk assessment of UVCBs (i.e., substances of unknown or variable composition, complex reaction products, or biological materials) is challenging due to their inherent complexity. A particular problem is that UVCBs can contain constituents with unidentified chemical structures and/or have variable composition of constituents from batch to batch. Moreover, the composition of a UVCB in the environment is not the same as that of the UVCB in a product, meaning that a risk assessment based on environmental exposure to the UVCB in a product does not represent the actual environmental risk. Here we propose an in silico fate-directed risk assessment framework for UVCBs using cedarwood oil as a case study. The framework uses Monte Carlo simulations and the mass-balance models SimpleTreat and RAIDAR to provide quantitative information on whether unidentified constituents within the physical-chemical property space of a UVCB can be the decisive factor for the environmental risk of the entire UVCB. Thereby the framework provides a robust decision tool to evaluate if a UVCB risk assessment requires additional tests or if the data on known constituents is representative for the risk of the entire UVCB. In the case of cedarwood oil, it could be shown that a risk assessment based on the known constituents (representing around 70% of the overall UVCB by weight) is representative for the environmental risk of the entire UVCB - reducing the need for additional testing and test animals.

CuO nanoparticles/Indonesian cedarwood essential oil‐loaded chitosan coating film: characterisation and antifungal improvement against Penicillium spp.

AbstractA novel formulation of composite coating comprising 0.8% chitosan (Chi) incorporating 0.025% CuO nanoparticles (CuO) and 0.5% Indonesian cedarwood essential oil (CEO) was fabricated by casting method. Fourier transform infrared, confocal laser scanning microscopy and scanning electron microscopy analyses were employed to characterise the biocompatibility of each formulation. Additionally, the physico‐chemically properties of the composite coatings were characterised. The colour (L*), light transmission, zeta potential and roughness of Chi were significantly (P < 0.05) altered negatively by the presence of CuO or CEO; the colour (a*, b* and ∆E), apparent viscosity and transparency also changed positively as a consequence of CuO and CEO incorporation. The antifungal features of a pure Chi coating against Penicillium italicum and Penicillium digitatum were improved synergistically by CuO and CEO, confirmed by in vitro and in vivo assays. Composite coatings obtained in this work may have potential applications for active primary food packaging, particularly for fresh postharvest commodities.

Multifactorial Evaluation of Relaxing Properties of Juniperus virginiana L. Essential Oil and Comparison with Salvia sclarea L. Essential Oil

Virginian cedarwood (Juniperus virginiana L.) essential oil is an important component of a very large number of fragrances, used in cosmetics or household products. Clary sage (Salvia sclarea L.) essential oil is well known in aromatherapy for its sedative property. The essential oils of Virginian cedarwood’s crushed barks and clary sage’s leaves were analyzed by GC/MS. Respectively, seven and seventeen compounds; representing 78% and 94.13% of the essential oils, were identified. The main components of the cedar oil are cedrene alpha (25%) and cedrol (20%). The main components of the clary sage oil are linalyl acetate (69.4%) and linalool (11.9%). Cedarwood oil exhibits significant relaxing activity, influencing physiological and biochemical parameters in human. This shows repercussions on situational stress. Virginian Cedarwood essential oil activity is compared with clary sage essential oil, which proved to be less efficient on stress relief.

Characteristics of two cedarwood essential oil emulsions and their antioxidant and antibacterial activities

Cedarwood essential oil (CEO) has the effect of anti-inflammatory and anti-bacteria. However, the application of this essential oil is limited due to its strong volatility and poor water solubility. To address this issue, two types of oil-in-water CEO emulsions including CEO nanoemulsion (CEO-NE) and CEO Pickering emulsion (CEO-PE) were prepared. CEO-NE with 5% surfactant, had a smaller particle size (135.14 ± 1.1 nm) and higher absolute zeta potential value (32.75 mV) compared with CEO-PE (1% starch) which particle size was 626.21 ± 6.05 nm, zeta potential was 27.58 mV. The stability of CEO-NE and CEO-PE were tested by multiple light scattering, results showed that Turbiscan Stability Index (TSI) value of CEO-NE was much lower than that of CEO-PE. CEO-NE and CEO-PE exerted higher free radical scavenging activities, iron reducing power and antibacterial ability than CEO itself. These results indicated that emulsification is a feasible method to extend application of CEO.

Impregnation of pectin-cedarwood essential oil nanocapsules onto mini cotton bag improves larvicidal performances

The use pesticide is one of the indispensable means to combat mosquito borne diseases. However, the repeated use of synthetic pesticides has induced resistance in the vector pest along with undesirable impact on the environment. The biodegradability, non-persistent and user’s safety are the root cause to prefer plant-derived pesticides to synthetic ones. The botanical based pesticides tend to degrade rapidly under the influence of several environmental factors. For the feasible application as pesticides, the plant products are formulated either as liquid or as purely solid. Despite well-established formulation technology in pesticide delivery, their handling trouble is being ignored. There is difficulty in liquid formulation of pesticide products, as they are prone to splashing and spillage, resulting in contamination, wastage and direct exposure to skin; whereas a solid formulation tends to produce dust. In the present work, cedarwood (Cedrus deodara) essential oil embedded pectin nanocapsules were produced. The nanocapsules were characterized according to their morphology, size, encapsulation efficiency and thermal stability. Furthermore, the nanocapsules were impregnated onto mini cotton tea bags to be employed as RTU (ready to use) formulation for treating the breeding sites of mosquitoes. The larvicidal activity of the bags treated with pectin-cedar wood nanocapsules was assessed against malaria vector, Anopheles culicifacies and 98% mortality was recorded till 4 weeks, this suggests its potential and hassle free applications in controlling mosquito vector.

The anxiolytic effect of Juniperus virginiana L. essential oil and determination of its active constituents

Essential oil from Juniperus virginiana L. (eastern red cedarwood essential oil, CWO) has been used to relax mind and enhance comfort for medical purposes. Few reports showed its effect on anxiety behaviors in animal models. The present study investigated the anxiolytic effect of CWO using two anxiety tests in mice, then determined the major active constituents, examined the change of neurotransmitters after intraperitoneal (i.p.) administration. Analysis using GC/MS revealed that the CWO contained (-)-α-cedrene (28.11%), (+)-β-cedrene (7.81%), (-)-thujopsene (17.71%) and (+)-cedrol (24.58%). CWO at 400–800mg/kg increased the percentage of open arm entries and the percentage of the time spent in open arms in the elevated plus maze (EPM), suggesting that the oil has anxiolytic effect. However, it didn't show anxiolytic effect in the light-dark box (LDB) test. Tests of the cedrene did not show anxiolytic effect in either test, but rather induced anxiety-related behaviors and inhibited the locomotor activity in EPM and LDB. Cedrol produced significant anxiolytic effect in both EPM and LDB tests at 400–1600mg/kg and 800–1600mg/kg, respectively. A more significant increase in locomotor activity was observed in cedrol at 200–1600mg/kg administration than CWO. CWO increased the 5-hydroxytryptamine (5-HT) concentration at 800mg/kg, whereas it didn't affect the dopamine (DA) concentration. Cedrol significantly reduced the DA level at 100-200mg/kg and elevated the 5-HT level at 1200–1600mg/kg. Moreover, it changed the ratio of 5-hydroxyindoleacetic acid/5-HT and 3, 4-dihydroxyphenyl acetic acid/DA at 1200–1600mg/kg. CWO and cedrol, in particular might act in an anxiolytic effect through the 5-HTnergic and DAnergic pathways.

EVALUATION OF THREE ESSENTIAL OILS ACTIVITIES AS ANTIMICROBIAL AND INSECTICIDAL AGENTS

The present work aimed to study the physicochemical properties, chemical composition antimicrobial and insecticied of three essential oils, namely cedar wood (Guniperus virginiana) belonging to famly (Pinaceae), rose wood (Aniba rosaeaodora) belonging to family (Rosaceae) and sandal wood (Santalum album) belonging to family (Santalaceae). These plants are considered to be medicinal and aromatic plants which are well grown in the Egyptian newly reclaimed lands. Evaluation of the oils activity as antimicrobial was done against Gram-positive and Gram-negative bacteria, fungi, yeast and larval fourth cotton leaf worm (Spodoptera littoralis). Our results showed that Cedar wood oil was distinguished by highest dextro rotation mean (+43o) while the specific gravity of sandal wood oil was higher than cedar wood oil and rose wood oil. The highest acid value recorded was cedar wood essential oil (2.45). The identified components in cedar wood essential oil were 20. The major compound was Thugopsene (27.5%), also the identified components in rose wood essential oil were 14. The major component was (Linalol 68.174%). As for the identified components in sandal wood essential oil, they were 13. The major component was α-Santalol (50.3%). Increasing the concentration of essential oils to 100% at least doubled the inhibition zone for most studied bacteria, except Escherichia coli. Also increasing the concentration of essential oils from (10:50%) increases the mortality of larvae , the death of the virgin and the proportion of altezr.

BIOCHEMICAL STUDIES ON SOME MEDICINAL AND AROMATIC PLANTS

The present investigation was carried out to study the chemical and physical characteristics of the essential oils produced from cedar wood, rose wood and sandal wood, these oils were also tested for their antimicrobial and insecticidal activity. The obtained results could be summarized as follows. The essential oils of cedar wood, rose wood and sandal wood were obtained from local market.The physico-chemical properties of such oils were determined to evaluate their values and application. Cedar wood was distinguished by highest dextro rotation (+43o) mean while the specific gravity of sandal wood was higher than cedar wood and rose wood. The highest acid value recorded to cedar wood essential oil (2.45).The chemical composition of essential oils were determined by gas chromatography - Mass spectrometry technique. The identified components in cedar wood essential oil were (20). The major compound was (Thugopsene 27.5%), also the identified components in rose wood essential oil were (14). The major component was(Linalol 68.174%), as for identified components in sandal wood essential oil were(13). The major component was (α-Santalol 50.3%).

Development and characterization of bioactive alginate microcapsules with cedarwood essential oil

AbstractIn this work, sodium alginate microcapsules containing cedarwood essential oil (CWO) for uses in anti‐acne tonic were prepared by ionic gelification of alginate with calcium chloride (CaCl2) and subsequent addition of glutaraldehyde to improve the crosslinking degree. Alginate microcapsules with cedarwood essential oil were obtained in an encapsulator with a 600 μm nozzle using different alginate concentrations (1, 3 and 4% w/v) , and different compositions of the coagulation solution with CaCl2 concentrations of 0.1, 0.25 and 0.5 M, and addition of glutaraldehyde at two concentrations: 12.5% and 50% (0.1 g L−1 and 10 g L−1, respectively). The effect of alginate concentration was followed by viscosimetry and the influence of the CaCl2 concentration and presence of glutaraldehyde on the microcapsules' shape as well as the total content on encapsulated cedarwood essential oil were evaluated by means of stereoscopic magnifying glass, scanning electron microscopy (SEM) and UV–vis spectrophotometry. Results show that proper shape formation is obtained for an alginate concentration of 3% w/v. With regard to the total encapsulated cedarwood oil, as the CaCl2 concentration in the gelifying‐coagulating solution increases the efficiency of the encapsulated oil. Best results in terms of micro‐bead shape and total amount of encapsulated oil were obtained for a CaCl2 concentration of 0.5 M without glutaraldehyde with a maximum encapsulation of 177.2 mg per gram of microcapsule.

Chemical Composition and Antibacterial Activity of the Essential of Cedrus atlantica (Cedarwood oil)

The purpose of this study was to determine the chemical composition and antibacterial activity of the Moroccan Cedarwood essential oil. The sawdust samples of Cedrus atlantica were collected from Azrou, located in the heart of The Middle Atlas region of Morocco. The plant samples were subjected to hydrodistillation for eight hours. The oils were then analysed by Gas Chromatography equipped with flame ionization detector (GC-FID) and Gas chromatography coupled with Mass spectrometry (GC-MS). The antimicrobial activities were tested in vitro on gram-negative bacteria Escherichia coli and Salmonella, as well as gram-positive Staphylococcus aureus, Bacillus subtilis and Bacillus cereus using the agar diffusion assay/aromatogramme and minimum inhibitory concentration (MIC). Three bacterial strains (Escherichia coli, Bacillus subtilis and Bacillus cereus) were found to be sensitive to cedarwood essential oil and showed effective bactericidal activity with a Minimum Inhibitory Concentration of 0,4 μl/ml for Escherichia coli and Bacillus cereus, and 0,2 μl/ml for Bacillus subtilis.

Antimicrobial activity of sugar beet lignocellulose films containing tung oil and cedarwood essential oil

Several studies have been reported on the use of cellulose, lignocellulose, chitin and other biolog- ical macromolecules for food packaging. One of the major drawbacks limiting their wide uses is their limited antimicrobial and water vapor barrier properties. In this study, cedarwood (Thuja occidentalis) essential oil (CWO), known to contain terpenes, polyphenols and tung oil, known to contain fatty acids, were used as additives to improve water vapor barrier, antimicrobial, and physical properties of laboratory cast lignocellulose films. Physicochemical and mechanical properties were tested to evaluate theimpact ofthe addition of oils in the filmsformulations.Theadditionof15 %CWOandtung oils improved water vapor permeability by more than 25 % as also evidenced by the increase of contact angle between water andfilm by 134 % from 38.98to 89.36 with 15 % tung oil. FTIR was used to monitor the presenceofhydrophobic groupsat 1463and1741 cm -1 from the oil on the film spectra confirming the improvement of film hydrophobicity from oils interac- tions. However, the addition of 15 % hydrophobic oils promoted a significant decrease in tensile properties of films (p B 0.05) by 40 % for CWO and 32 % for tung oil,respectively,duetoapartialincompatibilitybetween hydrophilic lignocellulose and hydrophobic oils. The use of a tailored coupling agent to reduce the incom- patibility and to improve the load charge transfer between hydrophobic and hydrophilic group might reduce the decrease of the tensile properties with the addition of hydrophobic compounds. No significant difference (p(0.05) was observed on the thermal stability of films using TGA. The antimicrobial effects of lignocellulose films against bacteria namely Listeria innocua, Escherichia coli ,a ndSalmonella enterica was tested by disk inhibition zone method and showed an improvement with the addition of 5 % CWO in thefilm. The Microbe Growth Index of the lignocellulose cellulosicfilmscontaining20 %w/wofCWOdecreases by 80 %. CWO and tung oil are good candidates to control microbial growth and water vapor permeability in flexible films. Future work will focus on the olfactory andtensilepropertiesforapplicationsinfoodpackaging.

천연 에센셜 오일의 주요 구성물질 분석과 항산화 및 항균 효과에 관한 연구

본 연구에서는 화장품산업에 이용 중인 천연 에센셜 오일 8종(라벤더, 티트리, 로즈마리, 주니퍼베리, 편백, 싸이프러스, 시더우드, 파인)을 가지고 물리 화학적 분석과 생리활성 및 항균효과를 실험하였다. 라벤더오일의 경우 에스테르 성분인 linalyl acetate가 48% 함유되어 있었으며, 5,000 ppm의 농도에서 22.36%의 항산화능을 보였다. 티트리 오일의 경우 0.5% 농도에서 43.94% 항산화능을 보였으며 항균효과 또한 뛰어났다. S. aureus, S. epidermidis, S. mutans, C. albicans 균에 각각 6, 3.5, 6.5, 5 mm의 저해환이 측정되어 매우 우수한 품질의 에센셜 오일임이 확인되었다. 편백 오일의 경우 pH가 2.64로 가장 산성도가 높았으며, 세스퀴테르펜 성분이 19.20%로 확인되었다. 시더우드 오일의 경우 샘플 중 비중과 굴절률이 가장 높았으며 전체성분의 99.73%가 세스퀴테르펜으로 구성되어 있었다. 또한 5,000 ppm 농도에서 39.68% 항산화능을 보였다. 특히 1% 농도에서 P. acnes, P. ovale, C. albicans 균에 각각 3.5, 6, 6 mm의 저해환이 측정되어 매우 탁월한 항균효과를 보였다. 반면 세스퀴테르펜 함량이 증가할수록 비교적 항산화능도 높게 나오는 경우도 있지만 그렇지 않은 경우도 있어서 성분조성보다는 각각의 항산화 성분 유무에 따라 항산화능이 결정된다고 판단되며, 마찬가지로 항균력 또한 테르펜의 성분조성보다는 각각의 항균성분의 유무에 따라 항균력이 결정된다고 사료된다. We studied the physical, chemical, biological, and antimicrobial effects of eight types of essential oils used in the cosmetics industry: lavender, tea tree, rosemary, juniper berry, Chamaecyparis obtusa, cypress, cedar wood, and pine. Lavender oil had a linalyl acetate (an ester chemical compound) content of 48% and radical scavenging activity of 22.36% at 5,000 ppm. Tea tree oil had radical scavenging activity of 43.94% at 5,000 ppm and antimicrobial activity against S. aureus, S. epidermidis, S. mutans, and C. albicans in each 6, 3.5, 6.5, and 5 mm, respectively. Chamaecyparis obtusa oil had the highest acidity (pH 2.64) compared with the other oils, and sesquiterpene compounds were found to have 19.20%. Cedar wood oil had the highest specific gravity and refractive index compared to the other oils and had a sesquiterpene content of 99.73%. The radical scavenging activity of cedar wood essential oil exceeded 39.68% at 5,000 ppm. The clear zone, indicating antimicrobial activity against P. acnes, P. ovale, and C. albicans, was 3.5, 6, and 6 mm, respectively, at a concentration of 1% cedar wood oil. Results showed that with a high sesquiterpene content, the antioxidant effect was generally, but not always, high, suggesting that this is determined according to composition of the compound rather than presence of each antioxidant. The results indicate that antimicrobial activity is determined by the existence of each antimicrobial ingredient rather than terpene composition.

Dual extraction of essential oil and podophyllotoxin from Juniperus virginiana

The leaves (needles) of eastern red cedar ( Juniperus virginiana L.) contain two important natural products: essential oil and podophyllotoxin. The hypothesis of this study was that it may be possible to extract both essential oil and podophyllotoxin from the leaves of the tree, by using a dual extraction method. Podophyllotoxin was obtained from the leaves following steam distillation of the leaves to produce the essential oil, indicating that steam distillation did not degrade podophyllotoxin. Furthermore, a product with 6% purity podophyllotoxin was obtained from the steam-distilled plant material, demonstrating the possibility for the establishment of an industrially economic protocol for dual extraction of these two natural products. Our study demonstrated that J. virginiana leaves, currently a waste-product from the timber industry, could be sequentially extracted for essential oil and podophyllotoxin and utilized as a by-product instead. We also found that the J. virginiana heartwood (a traditional source for cedarwood essential oil) does not contain podophyllotoxin. This is the first study to report both podophyllotoxin and essential oil in J. virginiana, and the first report on the dual extraction of these two natural products from the same biomass samples.

Microbial transformations 9. Biohydroxylation of alpha-cedrene and cedrol. Synthesis of an odoriferous minor component of cedar wood essential oil

The biohydroxylation of alpha-cedrene and of cedrol have been studied. These lead to products resulting from hydroxylation of non-activated carbon atoms, thus providing a rapid access to a potentially interesting minor component of Virginia Cedarwood Oil, which does display odoriferous properties.