CE Techniques Research Articles

Review of Six Different Next Generation Sequencing (NGS) Techniques for Forensic Science, including Advantages and Disadvantages

Next-generation sequencing methods have advanced greatly since Sanger sequencing, allowing for increased data yield, productivity, and utility. Read time can be used to categorize the upcoming technological generations. This article summarizes the differences between the two technological paradigms, the second-generation (short-read) kind, and the third-generation (long-read) variety. Popular technologies such as Ion Torrent and Illumina stand in for short-read sequencing methods, whereas Oxford Nanopore and Pacific Biosciences are used to represent long-read sequencing approaches. The introduction of the first next-generation sequencing (NGS) technology about ten years ago completely transformed the study of genetics. Whole genomes are now mapped and published practically weekly as a result of speed and cost advances. The number of scholarly papers and conference presentations highlighting the forensic uses of NGS in multiple forensic genetic laboratories has somewhat increased from the previous year. These results show that NGS provides new opportunities for forensic genomic investigation. To gather more information from multiple specimens in a single experiment, combinations of different markers, such as Short Tandem Repeats (STRs), Single Nucleotide Polymorphisms (SNPs), insertion/deletions, and mRNA, can be used instead of the usual Polymerase Chain Reactions- CE techniques. The most significant forensic STR loci's true spectrum of variation and hitherto unknown STR alleles have been discovered. We will address the possible use of singlemolecule sequencing and NGS in forensic science.

A comprehensive review of capillary electrophoresis-based techniques for erythropoietin isoforms analysis

Different CE techniques have been used to analyze erythropoietin. These techniques have been shown to be effective in differentiating and quantifying erythropoietin isoforms, including natural and recombinant origins. This review provides a comprehensive overview of various capillary electrophoresis-based techniques used for the analysis of erythropoietin isoforms. The importance of erythropoietin in clinical practice and the necessity for the accurate analysis of its isoforms are first discussed. Various techniques that have been used for erythropoietin isoform analysis are then described. The main body of the review focuses on the different capillary electrophoresis-based methods that have been developed for erythropoietin isoform analysis, including capillary zone electrophoresis and capillary isoelectric focusing. The advantages and drawbacks of each method as well as their applications are discussed. Suggestions into the future directions of the area are also described.

Effective mitigation of the belief perseverance bias after the retraction of misinformation: Awareness training and counter-speech.

The spread and influence of misinformation have become a matter of concern in society as misinformation can negatively impact individuals' beliefs, opinions and, consequently, decisions. Research has shown that individuals persevere in their biased beliefs and opinions even after the retraction of misinformation. This phenomenon is known as the belief perseverance bias. However, research on mitigating the belief perseverance bias after the retraction of misinformation has been limited. Only a few debiasing techniques with limited practical applicability have been proposed, and research on comparing various techniques in terms of their effectiveness has been scarce. This paper contributes to research on mitigating the belief perseverance bias after the retraction of misinformation by proposing counter-speech and awareness-training techniques and comparing them in terms of effectiveness to the existing counter-explanation technique in an experiment with N = 251 participants. To determine changes in opinions, the extent of the belief perseverance bias and the effectiveness of the debiasing techniques in mitigating the belief perseverance bias, we measure participants' opinions four times in the experiment by using Likert items and phi-coefficient measures. The effectiveness of the debiasing techniques is assessed by measuring the difference between the baseline opinions before exposure to misinformation and the opinions after exposure to a debiasing technique. Further, we discuss the efforts of the providers and recipients of debiasing and the practical applicability of the debiasing techniques. The CS technique, with a very large effect size, is the most effective among the three techniques. The CE and AT techniques, with medium effect sizes, are close to being equivalent in terms of their effectiveness. The CS and AT techniques are associated with less cognitive and time effort of the recipients of debiasing than the CE technique, while the AT and CE techniques require less effort from the providers of debiasing than the CS technique.

Sustainable strategic investment decision-making practices in UK companies: The influence of governance mechanisms on synergy between industry 4.0 and circular economy

Prior studies articulate the growing synergy between Industry 4.0 (I4.0) technologies and CE techniques. However, the literature lacks empirical evidence regarding boardrooms' commitment to implementing these technological domains through sustainable strategic investment decision-making practices (SSIDMP). This paper uses computer-aided textual analysis to explore the current trend of SSIDMP in UK companies. Our debate draws on data from UK companies, FTSEE ALL Share, over the period (2012−2021). We develop proxies for I4.0 technologies and CE techniques to examine the synergy between I4.0 technologies and CE techniques and the influence of this synergy on companies' financial performance. The findings reveal the vital role the organizational ambidexterity plays in the synergy between I4.0 technologies and CE techniques through exploring and exploiting technological techniques and mechanisms to cope with changing business environment. Our empirical study explores the impact of governance mechanisms on the relationships between SSIDMP, I4.0 technologies, CE techniques, and companies' financial performance. Findings support that synergy between these two domains is the key determinant of sustainable value creation and sustainable organizational performance. The synergy between I4.0 technologies and CE techniques significantly impact SSIDMP and companies' financial performance, though the level of the synergy varies according to industry sectors. Furthermore, our analysis shows that governance mechanisms (ESG, board compositions, risk management, internal audit) strengthen the relationships between these two domains and companies' performance.

The HURRIER process for experimentation in business‐to‐business mission‐critical systems

AbstractContinuous experimentation (CE) refers to a set of practices used by software companies to rapidly assess the usage, value, and performance of deployed software using data collected from customers and systems in the field using an experimental methodology. However, despite its increasing popularity in developing web‐facing applications, CE has not been studied in the development process of business‐to‐business (B2B) mission‐critical systems. By observing the CE practices of different teams, with a case study methodology inside Ericsson, we were able to identify the different practices and techniques used in B2B mission‐critical systems and a description and classification of the four possible types of experiments. We present and analyze each of the four types of experiments with examples in the context of the mission‐critical long‐term evolution (4G) product. These examples show the general experimentation process followed by the teams and the use of the different CE practices and techniques. Based on these examples and the empirical data, we derived the HURRIER process to deliver high‐quality solutions that the customers value. Finally, we discuss the challenges, opportunities, and lessons learned from applying CE and the HURRIER process in B2B mission‐critical systems.

Recent advances in capillary electrophoresis separation of monosaccharides, oligosaccharides, and polysaccharides

This article illustrates the basis and applications of methodologies for the analysis of simple and complex carbohydrates by means of CE. After a description of the most common and novel approaches useful for the analysis and characterization of carbohydrates, this review covers the recent advances in CE separation of monosaccharides, oligosaccharides, and polysaccharides. Various CE techniques are also illustrated for the study of carbohydrates derived from complex glyco-derivatives such as glycoproteins and glycolipids, essential for biopharmaceutical and glycoproteomics applications as well as for biomarker detection. Most glycans have no significant UV absorption, and derivatization with fluorophore groups prior to separation usually results in higher sensitivity and an improved electrophoretic profile. We also discuss the recent applications and separations by CE of derivatized simple and more complex carbohydrates with different chromophoric active tags. Overall, this review aims to give an overview of the most recent state-of-the-art techniques used in carbohydrate analysis by CE.

Nanoparticles and capillary electrophoresis: A marriage with environmental impact.

The impact of nanomaterials in the environment and human health is a cause of big concern and even though intensive studies are currently being carried out, there is still a lot to elucidate. The development of validated methods for the characterization and quantification of nanomaterials and their impact on the environment should be encouraged to achieve a proper, safe, and sustainable use of nanoparticles (NPs). Recently, CE emerged as a well-adapted technique for the analysis of environmental samples. This review presents the application of NPs together with CE systems for environmental pollutants analysis, as well as the application of CE techniques for the analysis of various types of NPs.

On-line two-dimensional capillary electrophoresis with mass spectrometric detection using a fully electric isolated mechanical valve.

CE is becoming more and more important in many fields of bioanalytical chemistry. Besides optical detection, hyphenation to ESI-MS detection is increasingly applied for sensitive identification purposes. Unfortunately, many CE techniques and methods established in research and industry are not compatible to ESI-MS since essential components of the background electrolyte interfere in ES ionization. In order to identify unknown peaks in established CE methods, here, a heart-cut 2D-CE separation system is introduced using a fully isolated mechanical valve with an internal loop of only 20 nL. In this system, the sample is separated using potentially any non-ESI compatible method in the first separation dimension. Subsequently, the portion of interest is cut by the internal sample loop of the valve and reintroduced to the second dimension where the interfering compounds are removed, followed by ESI-MS detection. When comparing the separation efficiency of the system with the valve to a system using a continuous capillary only a slight increase in peak width is observed. Ultraviolet/visible detection is integrated in the first dimension for switching time determination, enabling reproducible cutting of peaks of interest. The feasibility of the system is successfully demonstrated by a 2D analysis of a BSA tryptic digest sample using a nonvolatile (phosphate based) background electrolyte in the first dimension.

Recent developments in capillary and microchip electroseparations of peptides (2013-middle 2015).

The review brings a comprehensive survey of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, micropreparation, purification, and physicochemical and biochemical characterization of peptides in the years 2013, 2014, and ca. up to the middle of 2015. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are described. New developments in particular CE and CEC modes are presented and several types of their applications to peptide analysis are reported: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence, and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide important physicochemical characteristics of peptides are demonstrated.

Human Vection Perception Using Inertial Nulling and Certainty Estimation: The Effect of Migraine History

Vection is an illusory perception of self-motion that can occur when visual motion fills the majority of the visual field. This study examines the effect of the duration of visual field movement (VFM) on the perceived strength of self-motion using an inertial nulling (IN) and a magnitude estimation technique based on the certainty that motion occurred (certainty estimation, CE). These techniques were then used to investigate the association between migraine diagnosis and the strength of perceived vection. Visual star-field stimuli consistent with either looming or receding motion were presented for 1, 4, 8 or 16s. Subjects reported the perceived direction of self-motion during the final 1s of the stimulus. For the IN method, an inertial nulling motion was delivered during this final 1s of the visual stimulus, and subjects reported the direction of perceived self-motion during this final second. The magnitude of inertial motion was varied adaptively to determine the point of subjective equality (PSE) at which forward or backward responses were equally likely. For the CE trials the same range of VFM was used but without inertial motion and subjects rated their certainty of motion on a scale of 0–100. PSE determined with the IN technique depended on direction and duration of visual motion and the CE technique showed greater certainty of perceived vection with longer VFM duration. A strong correlation between CE and IN techniques was present for the 8s stimulus. There was appreciable between-subject variation in both CE and IN techniques and migraine was associated with significantly increased perception of self-motion by CE and IN at 8 and 16s. Together, these results suggest that vection may be measured by both CE and IN techniques with good correlation. The results also suggest that susceptibility to vection may be higher in subjects with a history of migraine.

Capillary electrophoretic methods for the screening and determination of pharmacologic adulterants in herbal-based pharmaceutical formulations.

This review aims to describe the CE methods utilized for the determination of adulterants in herbal-based formulations marketed for different therapeutic purposes. The CE methods for screening and determination of pharmacologic adulterants are reviewed on the basis of the CE techniques and their detection methods. CZE and MEKC methods coupled to optical (UV), capacitively coupled contactless conductivity, and MS detection modes are discussed and reviewed. Worldwide adulteration cases related to pharmaceutical formulations containing herbs as the main active products are presented covering all the works published in the last four decades.

Novel 3-hydroxypropyl-bonded phase by direct hydrosilylation of allyl alcohol on amorphous hydride silica.

A novel 3-hydroxypropyl (propanol)-bonded silica phase has been prepared by hydrosilylation of allyl alcohol on a hydride silica intermediate, in the presence of platinum (0)-divinyltetramethyldisiloxane (Karstedt's catalyst). The regio-selectivity of this synthetic approach had been correctly predicted by previous reports involving octakis(dimethylsiloxy)octasilsesquioxane (Q8 M8 (H) ) and hydrogen silsesquioxane (T8 H8 ), as molecular analogs of hydride amorphous silica. Thus, C-silylation predominated (∼94%) over O-silylation, and high surface coverages of propanol groups (5 ± 1 μmol/m(2) ) were typically obtained in this work. The propanol-bonded phase was characterized by spectroscopic (infrared (IR) and solid-state NMR on silica microparticles), contact angle (on fused-silica wafers) and CE (on fused-silica tubes) techniques. CE studies of the migration behavior of pyridine, caffeine, Tris(2,2'-bipyridine)Ru(II) chloride and lysozyme on propanol-modified capillaries were carried out. The adsorption properties of these select silanol-sensitive solutes were compared to those on the unmodified and hydride-modified tubes. It was found that hydrolysis of the SiH species underlying the immobilized propanol moieties leads mainly to strong ion-exchange-based interactions with the basic solutes at pH 4, particularly with lysozyme. Interestingly, and in agreement with water contact angle and electroosmotic mobility figures, the silanol-probe interactions on the buffer-exposed (hydrolyzed) hydride surface are quite different from those of the original unmodified tube.

Buffer salt effects in off-line coupling of capillary electrophoresis and mass spectrometry.

In this work, the impact of buffer salts/matrix effects on the signal in direct injection MS with an electrospray interface (DI-ESI-MS) following pITP fractionation of the sample was studied. A range of buffers frequently used in CE analyses (pH 3-10) was prepared containing 10, 50, and 90% v/v of ACN, respectively. The sets of calibration solutions of cetirizine (an antihistaminic drug with an amphiprotic character) within a 0.05-2.0 mg/L concentration range were prepared in different buffers. The greatest enhancements in the MS signal (in terms of change in the slope of the calibration line) were obtained for the beta-alanine buffer (pH 3.5) in positive ionization and for the borate buffer (pH 9.2) in negative ionization, respectively. The procedure was successfully applied to the analysis of buserelin (a peptidic drug). The slope of the calibration line for solutions containing the beta-alanine buffer with 50% of ACN was 4 times higher than for water or urine, respectively. This study clearly demonstrates that the buffer salt/matrix effects in an offline combination of pITP and DI-ESI-MS can also play a positive role, as they can enhance the signal in MS. A similar influence of the above effects can also be presumed in the CE techniques combined on-line with ESI-MS.

Use of imaged capillary isoelectric focusing technique in development of diphtheria toxin mutant CRM197

Polysaccharide based-vaccines have been successful in providing protection in adults from bacterial infections, however they are not as effective in infants or young children. To enhance the immune response in these high risk groups, the polysaccharide is conjugated with a carrier protein such as cross-reacting material 197 (CRM197). The CRM197 protein has been well-characterized biochemically and biophysically using various analytical techniques however, none of these have been CE-based methods. Of the various CE techniques, imaged capillary isoelectric focusing (icIEF) is a method that has been used extensively in the field of protein-based drug development as a tool for product identification, stability monitoring, and characterization. Applications of icIEF technique using Convergent Bioscience icIEF instrumentation with whole-field imaging technology are presented and discussed in this paper. These applications include rapid method development to establish a CRM197 identity test for product release, a concentration assay for upstream and downstream in-process product development, and CRM197 stability with respect to its charge heterogeneity under accelerated temperature stress. The data presented demonstrates the utility of the icIEF method as a multifunctional assay because it can screen for better product candidates during early stage clonal selection as well as support in-process and final product characterization throughout CRM197 development.

Channel State Information Estimation In MIMO-OFDM Wireless Systems

Multiple Input Multiple Output (MIMO) in combination with Orthogonal Frequency Division Multiplexing (OFDM) can provide spectrally efficient and ISI free communication. Channel estimation is of great importance in order to recover the signal at the receiver side. Therefore accurate channel state information is essential for proper detection and decoding in MIMO-OFDM wireless systems. To estimate channel state information various types of techniques are being deployed in these systems. Accuracy and precision of channel estimation depends on the techniques used for the purpose of estimating channel state information. The more the accuracy of the technique, more will be the accurate performance of the system. In this paper we provide a review of the various types of techniques that are used in estimation of channel in MIMO-OFDM systems. Although the existing CE techniques for OFDM can be employed for MIMO-OFDM systems too but the computation complexity is increased in the latter. The techniques can be chosen on the basis of their performance which can be judged by Mean Square Error (MSE), convergence speed, and the stability of the algorithm. Various CE techniques like LS and MMSE can be used. Moreover adaptive CE techniques like LMS, NLMS, RLS are gaining more attention. These can be chosen based on the system requirement and the environment in which the system is to be deployed. Comparison of the techniques on the basis of above given parameters is shown in this paper. Also the improvement aspects for techniques of channel estimation are discussed.

Recent advances in capillary electrophoretic migration techniques for pharmaceutical analysis

Since the introduction about 30 years ago, CE techniques have gained a significant impact in pharmaceutical analysis. The present review covers recent advances and applications of CE for the analysis of pharmaceuticals. Both small molecules and biomolecules such as proteins are considered. The applications range from the determination of drug-related substances to the analysis of counterions and the determination of physicochemical parameters. Furthermore, general considerations of CE methods in pharmaceutical analysis are described.

Recent developments in capillary and microchip electroseparations of peptides (2011–2013)

The review presents a comprehensive survey of recent developments and applications of capillary and microchip electroseparation methods (zone electrophoresis, ITP, IEF, affinity electrophoresis, EKC, and electrochromatography) for analysis, isolation, purification, and physicochemical and biochemical characterization of peptides. Advances in the investigation of electromigration properties of peptides, in the methodology of their analysis, including sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, as well as in detection of peptides, are presented. New developments in particular CE and CEC modes are reported and several types of their applications to peptide analysis are described: conventional qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC techniques to provide relevant physicochemical characteristics of peptides are demonstrated.

Separation of enantiomers of norephedrine by capillary electrophoresis using cyclodextrins as chiral selectors: Comparative CE and NMR studies

In this study, the enantiomer migration order (EMO) of norephedrine (NEP) in the presence of various CDs was investigated by CE. NMR and CE techniques were used to analyze the mechanism of the chiral recognition between NEP enantiomers and four CDs, i.e., native α-CD, β-CD, heptakis(2,3-di-O-acetyl-6-O-sulfo)-β-CD (HDAS-β-CD), and heptakis(2,3-di-O-methyl-6-O-sulfo)-β-CD (HDMS-β-CD). EMO was reversed in the presence of α-CD and β-CD, although only minor differences in the structures of the complexes formed between NEP and these CDs could be derived from rotating frame nuclear Overhauser experiments (ROESY). The complexes between the enantiomers of NEP and the sulfated CDs, HDMS-β-CD, and HDAS-β-CD, were substantially different. However, EMO of NEP was identical in the presence of these CDs. HDAS-β-CD proved to be the most suitable chiral selector for the CE enantioseparation of NEP.

Rapid screening of poly(ethylene glycol) polymers by C18 column-flow injection with piezoelectric detection system

A rapid and low cost analytical method for the screening and determination of poly(ethylene glycol)s polymers has been developed. Several initial studies were carried out in order to demonstrate the utility of a piezoelectric quartz crystal as a detection system for the quantification of poly(ethylene glycol)s. The method involves the use of a simple flow injection device connected to a piezoelectric quartz crystal detector. Several parameters associated with the simple flow injection manifold were optimized. Subsequently, this detector was coupled to a C18 column and the best experimental conditions were investigated in order to perform the screening and determination of poly(ethylene glycol)s. The applicability of this method, as an analytical screening tool, was demonstrated by the characterization of samples with different molecular mass polymers without using HPLC and CE techniques to carry out oligomer separations. A standard curve to determine the polymer content was obtained by relating the resonance frequency shift and polymer concentration, which showed good linearity and good regression coefficients. The working range, repeatability, limit of detection and recovery were all acceptable for the analysis of the poly(ethylene glycol)s. Finally, the proposed method was applied to determine these polymers in several samples, such as pharmaceutical products, washing powder, detergents and a body spray.

Public participation in commercial environments: critical reflections on community engagement methods used in the Australian plantation forestry industry

Summary Social concerns surrounding commercial plantation forest management practices in Australia have resulted in calls for more participatory forms of forest management decision-making. Public participation (or community engagement, CE) processes provide opportunities for affected and interested community members to voice their concerns over proposed plantation management activities, share relevant information and influence decision-making processes. A large body of literature provides ample support for the adoption of more participatory forms of resource management. The literature, however, provides little guidance on implementing such processes within the commercial domain of plantation forest management. Based on a review of the public participation literature and key informant interviews, we highlight the gaps between CE in practice in Australian commercial plantation management, and the theoretical objectives of CE: trust, process flexibility, inclusivity and representation. These gaps stem from the need to implement CE techniques in a way that recognises the commercial and regulatory realities of plantation forest management. While participatory techniques currently used by plantation managers meet some of the ideal objectives of ‘good’ participation and there is room for improvement, this improvement can take place only if CE practitioners recognise and address the commercial realities of CE implementation within plantation management, and acknowledge the limited practical applicability of some theoretical objectives.