Cd Influx Research Articles

Cadmium-induced protein AS8: a protein to improve Cd accumulation and transport via Cd uptake in poplar

The pollution of soil with heavy metals (HMs) has become an environmental problem of global concern. Phytoremediation, whereby plants extract HMs from soil, can efficiently and substantially reduce HM pollution in soil in an environmentally friendly manner. Cadmium-induced protein AS8 (CIPAS8) is present in many plants and its expression is induced by HMs. In this study, PeCIPAS8 and SlCIPAS8 were transformed into 84K poplar to study their effects on tolerance to, and translocation of, cadmium (Cd) in woody plants. Localization analyses showed that two CIPAS8 proteins were localized at the plasma membrane when transiently expressed in tobacco leaf epidermal cells. Compared with wild-type 84K poplar seedlings, transgenic poplar lines overexpressing PeCIPAS8 or SlCIPAS8 showed increased Cd contents and decreased Cd tolerance. Transgenic poplar lines overexpressing PeCIPAS8 or SlCIPAS8 accumulated more Cd in the roots, stems, and leaves, but the plant height did not differ significantly, compared with wild-type 84K poplar under Cd stress during the vegetative stage. CIPAS8 increased the Cd influx rate of transgenic poplar roots compared with that of the wild type, and affected the transcription levels of other metal transporters. These findings show that CIPAS8 increases Cd flux into plant tissues and demonstrate moderate Cd sensitivity of the plant. Therefore, CIPAS8 is an influx transporter with the potential to increase the uptake of toxic HMs by woody plants growing in HM-contaminated soils.

Peroxidase in plant defense: Novel insights for cadmium accumulation in rice (Oryza sativa L.)

Phenylpropanoid biosynthesis plays crucial roles in the adaptation to cadmium (Cd) stress. Nevertheless, few reports have dabbled in physiological mechanisms of such super pathway regulating Cd accumulation in plants. Herein, by integrating transcriptomic, histological and molecular biology approaches, the present study dedicated to clarify molecular mechanism on how rice adapt to Cd stress via phenylpropanoid biosynthesis. Our analysis identified that the enhancement of phenylpropanoid biosynthesis was as a key response to Cd stress. Intriguingly, POD occupied a significant part in this process, with the number of POD related genes accounted for 26/29 of all upregulated genes in phenylpropanoid biosynthesis. We further used SHAM (salicylhydroxamic acid, the POD inhibitor) to validate that POD exhibited a negative correlation with the Cd accumulation in rice tissues, and proposed two intrinsic molecular mechanisms on POD in contributing to Cd detoxification. One strategy was that POD promoted the formation of lignin and CSs both in endodermis and exodermis for intercepting Cd influx. In detail, inhibited POD induced by external addition of SHAM decreased the content of lignin by 50.98–66.65 % and delayed percentage of the DTIP-CS to root length by 39.17–104.51 %. The other strategy was expression of transporter genes involved in Cd uptake, including OsIRT1, OsIRT2, OsZIP1 and OsZIP, negatively regulated by POD. In a word, our findings firstly draws a direct link between POD activity and the Cd accumulation, which is imperative for the breeding of rice with low-Cd-accumulating capacity in the future.

Comparative effects of silicon and silicon nanoparticles on the antioxidant system and cadmium uptake in tomato under cadmium stress

Cadmium (Cd) pollution is an important threat to agricultural production globally. Silicon (Si) and silicon nanoparticles (Si NPs) can mitigate Cd stress in plants. However, the mechanisms underlying the impacts of Si and Si NPs on Cd resistance, particularly in low-Si accumulators, remain inadequately understood. Accordingly, we conducted a comparative investigation into the roles of Si and Si NPs in regulating the antioxidant system (enzymes and antioxidants) and Cd uptake (influx rate, symplastic and apoplastic pathways) in tomato (a typical low-Si accumulator). The results revealed that Si and Si NPs improved tomato growth under Cd stress, and principal component analysis (PCA) demonstrated that Si NPs were more effective than Si. For oxidative damage, redundancy analysis (RDA) results showed that Si NPs ameliorated oxidative damage in both shoots and roots, whereas Si predominantly alleviated oxidative damage in roots. Simultaneously, Si and Si NPs regulated antioxidant enzymes and nonenzymatic antioxidants with distinct targets and strengths. Furthermore, Si and Si NPs decreased Cd concentration in tomato shoot, root, and xylem sap, while Si NPs induced a more significant decline in shoot and xylem sap Cd. Noninvasive microtest and quantitative estimation of trisodium-8-hydroxy-1,3,6-pyrenetrisulfonic (PTS, an apoplastic tracer) showed that Si and Si NPs reduced the Cd influx rate and apoplastic Cd uptake, while Si NPs induced a more significant reduction. Moreover, Si regulated the expression of genes responsible for Cd uptake (NRAMP2 and LCT1) and compartmentalization (HMA3), while Si NPs reduced the expression of NRAMP2. In conjunction with RDA, the results showed that Si and Si NPs decreased Cd uptake mainly by regulating the symplastic and apoplastic pathways, respectively. Overall, our results indicate that Si NPs is more effective in promoting tomato growth and alleviating oxidative damage than Si in tomato under Cd stress by modulating the antioxidant system and reducing apoplastic Cd uptake.

Inventory of cadmium-transporter genes in the root of mangrove plant Avicennia marina under cadmium stress

Mangrove Avicennia marina has the importantly potential for cadmium (Cd) pollution remediation in coastal wetlands. Unfortunately, the molecular mechanisms and transporter members for Cd uptake by the roots of A. marina are not well documented. In this study, photosynthetic and phenotypic analysis indicated that A. marina is particularly tolerant to Cd. The content and flux analysis indicated that Cd is mainly retained in the roots, with greater Cd influx in fine roots than that in coarse roots, and higher Cd influx in the root meristem zone as well. Using transcriptomic analysis, a total of 5238 differentially expressed genes were identified between the Cd treatment and control group. Moreover, we found that 54 genes were responsible for inorganic ion transport. Among these genes, AmHMA2, AmIRT1, and AmPCR2 were localized in the plasma membrane and AmZIP1 was localized in both plasma membrane and cytoplasm. All above gene encoding transporters showed significant Cd transport activities using function assay in yeast cells. In addition, the overexpression of AmZIP1 or AmPCR2 in Arabidopsis improved the Cd tolerance of transgenic plants. This is particularly significant as it provides insight into the molecular mechanism for Cd uptake by the roots of mangrove plants and a theoretical basis for coastal wetland phytoremediation.

Divergent effects of cerium oxide nanoparticles alone and in combination with cadmium on nutrient acquisition and the growth of maize (Zea mays).

The increasing use of cerium nanoparticles (CeO2-NPs) has made their influx in agroecosystems imminent through air and soil deposition or untreated wastewater irrigation. Another major pollutant associated with anthropogenic activities is Cd, which has adverse effects on plants, animals, and humans. The major source of the influx of Cd and Ce metals in the human food chain is contaminated food, making it an alarming issue; thus, there is a need to understand the factors that can reduce the potential damage of these heavy metals. The present investigation was conducted to evaluate the effect of CeO2-10-nm-NPs and Cd (alone and in combination) on Zea mays growth. A pot experiment (in sand) was conducted to check the effect of 0, 200, 400, 600, 1,000, and 2,000 mg of CeO2-10 nm-NPs/kg-1 dry sand alone and in combination with 0 and 0.5 mg Cd/kg-1 dry sand on maize seedlings grown in a partially controlled greenhouse environment, making a total of 12 treatments applied in four replicates under a factorial design. Maize seedling biomass, shoot and root growth, nutrient content, and root anatomy were measured. The NPs were toxic to plant biomass (shoot and root dry weight), and growth at 2,000 ppm was the most toxic in Cd-0 sets. For Cd-0.5 sets, NPs applied at 1,000 ppm somewhat reverted Cd toxicity compared with the contaminated control (CC). Additionally, CeO2-NPs affected Cd translocation, and variable Ce uptake was observed in the presence of Cd compared with non-Cd applied sets. Furthermore, CeO2-NPs partially controlled the elemental content of roots and shoots (micronutrients such as B, Mn, Ni, Cu, Zn, Mo, and Fe and the elements Co and Si) and affected root anatomy.

The overexpression of LOW PHOSPHATE ROOT 1 (LPR1) negatively regulates Arabidopsis growth in response to Cadmium (Cd) stress

Cadmium (Cd) is a highly toxic element that is easily absorbed by plant, and the mechanisms of the plant response to Cd toxicity are very complex. In this study, the role of LPR1 (LOW Phosphate Root 1) encoding a cell-wall-targeted ferroxidase in Cd stress was investigated. The results showed that the overexpression of LPR1 caused an average reduction of 23%–40% in the primary root lengths, 67%–73% in the fresh weights, 32%–46% in the lengths of the non-root hair zone (NRHZ) and 70%–71% in the chlorophyll contents in both LPR1-OX lines when compared with the wild type (WT), while there were no significant changes in these traits between the WT and mutant lpr1 lines under Cd stress (7.5 μmol/L CdSO4). Further investigation showed that the overexpression of LPR1 triggered reactive oxygen species (ROS) bursts and reduced the entry of available iron (Fe2+) into the cell, which induced the expression of iron-regulated transporter 1 (IRT1). The up-regulation of IRT1 contributed to the increase of Cd accumulation and growth retardation under Cd stress. Exogenous Fe and ROS scavengers down-regulated the IRT1's expression and alleviated the growth inhibition in LPR1-OX lines, indicating that LPR1-dependent ROS up-regulated IRT1, which subsequently exacerbated the Cd influx into plants. Our findings highlight a pathway of LPR1-mediated plant responding to Cd toxicity stress through the regulation of ROS and Fe homeostasis.

Sly-miR398 Participates in Cadmium Stress Acclimation by Regulating Antioxidant System and Cadmium Transport in Tomato (Solanum lycopersicum).

Cadmium (Cd) pollution is one of the major threats in agricultural production, and can cause oxidative damage and growth limitation in plants. MicroRNA398 (miR398) is involved in plant resistance to different stresses, and the post-transcriptional regulation of miR398 on CSDs plays a key role. Here, we report that miR398 was down-regulated in tomato in response to Cd stress. Simultaneously, CSD1 and SOD were up-regulated, with CSD2 unchanged, suggesting CSD1 is involved in miR398-induced regulation under Cd stress. In addition, the role of miR398 in Cd tolerance in tomato was evaluated using a transgenic line overexpressing MIR398 (miR398#OE) in which the down-expression of miR398 was disrupted. The results showed that Cd stress induced more significant growth inhibition, oxidative damage, and antioxidant enzymes disorder in miR398#OE than that in wild type (WT). Moreover, higher Cd concentration in the shoot and xylem sap, and net Cd influx rate, were observed in miR398#OE, which could be due to the increased Cd uptake genes (IRT1, IRT2, and NRAMP2) and decreased Cd compartmentalization gene HMA3. Overall, our results indicate that down-regulated miR398 plays a protective role in tomato against Cd stress by modulating the activity of antioxidant enzymes and Cd uptake and translocation.

VrNramp5 is responsible for cadmium and manganese uptake in Vigna radiata roots

As a non-essential mineral, Cadmium inhibits the growth and development of plants. A cultivar (Sulu, SL as wild type) of mungbean is more sensitive to Cd toxicity than its mutant line (20#). However, there is little information about the underlying mechanism. In this study, mutant line (20#) of mungbean exhibited lower Cd accumulation than wild type SL based on the time-course and dose-dependent Cd content in mungbean roots. Meanwhile, compared to 20# roots, the root tips of SL possess a higher capacity for transient Cd influx during exposure to Cd alone or co-treatment with Cd and manganese (Mn). Cd exposure reduced Mn contents of roots in both SL and 20#. Likewise, the addition of Mn in solution or in Cd-polluted soils inhibited Cd uptake in roots. Such reciprocal inhibition shows that Cd competes with Mn for uptake in roots of mungbean. Comparative transcriptomic of roots in SL and 20# were analyzed by Illumina sequencing to elucidate the molecular mechanisms of competitive uptake between Cd and Mn for mungbean. The transcriptomic analysis demonstrated that 28 metal transporters genes showed the log 2 fold changes under Cd stress, including ABC, ZIP, CAX, CCX, MTP, and Nramp family members. Expression abundance of VrABCC15–1 and VrZIP8 genes were significant up-regulated, and VrNramp5 was down-regulated in roots of mungbean under Cd stress. Thus, the three genes were complementarily expressed in Cd sensitive yeast (Δycf1). Expression of VrNramp5 resulted in hypersensitivity to excess Cd owing to the increment of Cd uptake in yeasts during exposure to Cd, but not by VrABCC15–1 or VrZIP8. Expressing-VrNramp5-SL or -VrNramp5–20# yeasts also accumulate higher Mn than that of the expressing-empty vector yeasts. The findings of this study demonstrated that the differences of Cd accumulation between SL and 20# was due to Cd uptake differences, and VrNramp5 is involved in Mn and Cd uptake in mungbean roots.

Metalloprotein-Specific or Critical Amino Acid Residues: Perspectives on Plant-Precise Detoxification and Recognition Mechanisms under Cadmium Stress.

Cadmium (Cd) pollution in cultivated land is caused by irresistible geological factors and human activities; intense diffusion and migration have seriously affected the safety of food crops. Plants have evolved mechanisms to control excessive influx of Cd in the environment, such as directional transport, chelation and detoxification. This is done by some specific metalloproteins, whose key amino acid motifs have been investigated by scientists one by one. The application of powerful cell biology, crystal structure science, and molecular probe targeted labeling technology has identified a series of protein families involved in the influx, transport and detoxification of the heavy metal Cd. This review summarizes them as influx proteins (NRAMP, ZIP), chelating proteins (MT, PDF), vacuolar proteins (CAX, ABCC, MTP), long-distance transport proteins (OPT, HMA) and efflux proteins (PCR, ABCG). We selected representative proteins from each family, and compared their amino acid sequence, motif structure, subcellular location, tissue specific distribution and other characteristics of differences and common points, so as to summarize the key residues of the Cd binding target. Then, we explain its special mechanism of action from the molecular structure. In conclusion, this review is expected to provide a reference for the exploration of key amino acid targets of Cd, and lay a foundation for the intelligent design and breeding of crops with high/low Cd accumulation.

Radial transport difference mediated by root endodermal barriers contributes to differential cadmium accumulation between japonica and indica subspecies of rice (Oryza sativa L.)

Although Cd concentration of grains is generally lower in japonica than in indica subspecies, the effects of root endodermal barriers on the subspecific differences in Cd accumulation in rice (Oryza sativa L.) are poorly understood. Here, we characterized the differences in endodermal differentiation between japonica and indica subspecies and their effects on Cd radial transport. Casparian strips (CSs) and suberin lamellae (SL) in japonica subspecies were initiated at the 6%− 7% and 21%− 27% position from the root tip, respectively, which were 65% and 26% earlier than in indica subspecies, respectively. The lignin/suberin content in japonica subspecies was 47%/42% greater than that in indica subspecies because of the higher expression of lignin/suberin biosynthesis-related genes (OsCASP1, OsPAL, OsCYP86A1 and OsKCS20). Cd exposure induced endodermal plasticity in both subspecies, but the changes in japonica were greater than in indica subspecies. The earlier formation of CSs/SL in japonica subspecies significantly restricted the flow of radial transport tracer to reach the xylem and decreased Cd influx into roots, that is, endodermal barriers inhibited Cd radial transport via both apoplastic and cell-to-cell pathways, thus decreasing the root-to-shoot transport of Cd in japonica subspecies. Our findings are beneficial for the genetic modification of rice with low-Cd-accumulating ability.

Ammonium has stronger Cd detoxification ability than nitrate by reducing Cd influx and increasing Cd fixation in Solanum nigrum L.

Cadmium (Cd) is a harmful heavy metal that affects the growth and development of plants. Nitrogen (N) is an essential nutrient for plants, and appropriate N management can improve Cd tolerance. The aim of our study was to explore the effects of different forms of N on the molecular and physiological responses of the hyperaccumulator Solanum nigrum to Cd toxicity. Measurement of biomass, photosynthetic parameters, and Cd2+ fluxes using non-invasive micro-test technique, Cd fluorescent dying, biochemical methods and quantitative real-time PCR analysis were performed in our study. Our results showed that ammonium (NH4+) has stronger Cd detoxification ability than nitrate (NO3-), which are likely attributed to the following three reasons: (1) NH4+ decreased the influx and accumulation of Cd2+ by regulating the transcription of Cd transport-related genes; (2) the ameliorative effects of NH4+ were accompanied by the increased retention of Cd in the cell walls of roots; and (3) NH4+ up-regulated SnExp expression.

Harnessing an arbuscular mycorrhizal fungus to improve the adaptability of a facultative metallophytic poplar (Populus yunnanensis) to cadmium stress: Physiological and molecular responses

Populus yunnanensis Dode, a facultative metallophytic poplar, exhibits afforestation potential in barren mine tailing areas. However, the interactions and functional roles of arbuscular mycorrhizal fungus (AMF) in P. yunnanensis adaptability to heavy metal stress remain unclear. Physiological and molecular responses of P. yunnanensis plantlets to AMF (Funneliformis mosseae) under cadmium (Cd) stress (50 mg kg−1) were investigated. Results showed attenuation of Cd phytotoxicity effects on cell organelles upon AMF inoculation, which also reduced the Cd concentration in the poplar leaves, stems, and roots. Under Cd stress, AMF-blocking of metal transporter (e.g., Ca2+ channel) activity occurred, decreasing root cell Cd influx by reducing H+ efflux. Bioaugmentation of rhizosphere sediments by AMF to stabilize metals with a decreasing DTPA-extractable Cd also occurred. The AMF inoculation promoted Cd conversion into inactive, less phytotoxic forms, and helped to maintain ion homeostasis and relieve nutritional ion (e.g., Ca, Mg) disorders caused by excessive Cd. Leaf enzyme and non-enzyme antioxidant systems were triggered. Root and leaf physiological response patterns differed. The AMF regulated the poplar functional genes, and nine metal-responsive gene clusters were identified. We suggest that AMF is a functional component of P. yunnanensis phenotype extension, contributing to strong adaptability to unfavorable mine tailings conditions.

Effects of sulfur application on cadmium accumulation in brown rice under wheat-rice rotation

We investigated how sulfur (S) application prior to wheat cultivation under wheat-rice rotation influences the uptake of cadmium (Cd) in rice grown in low- and high-Cd soils. A pot experiment was conducted with four S levels (0, 30, 60, 120 mg S kg−1) and two Cd rates (low and high, 0.35 and 10.35 mg Cd kg−1) supplied to wheat. Part of the wheat straw was returned to the soil before planting rice, which was cultivated for 132 days. To explore the key mechanisms by which S application controlled Cd accumulation in brown rice, (1) soil pore water at the key growth stages was sampled, and dissolved Cd and S species concentrations were determined; (2) rice plant tissues (including iron plaque on the root surface) were sampled at maturity for Cd and S analysis. With increasing S level, Cd accumulation in brown rice peaked at 60 mg S kg−1, irrespective of soil Cd levels. For high-Cd soils, concentrations of Cd in brown rice increased by 57%, 228%, and 100% at 30, 60, and 120 mg S kg−1, respectively, compared with no S treatment. The increase in brown rice Cd by low S levels (0–60 mg kg−1) could be attributed to (1) the S-induced increase in soil pore water sulfate increasing the Cd influx into rice roots and (2) the S-induced increase in leaf S promoting Cd translocation into brown rice. However, brown rice Cd decreased at 120 mg S kg−1 due to (1) low Cd solubility at 120 mg S kg−1 and (2) root and leaf S uptake, which inhibited Cd uptake. Sulfur application to wheat crop increased the risk of Cd accumulation in brown rice. Thus, applying S-containing fertilizers to Cd-contaminated paddy soils is not recommended.

BcNRAMP1 promotes the absorption of cadmium and manganese in Arabidopsis

Cadmium (Cd) is a toxic nonessential metal that poses a health risk for humans. Cd is easily accumulated in leaf vegetables than in other vegetables. Leafy vegetables are one of the major dietary Cd sources for the human body. In this study, pak choi was used as our experimental material as it is an important leafy vegetable, especially in Asia. A NRAMP transporter – BcNRAMP1 was identified in pak choi, which is involved in manganese (Mn) and Cd uptake in yeast and in planta. BcNRAMP1 is expressed in the whole plant body of pak choi, with a higher abundance in root tissues than in shoots. Mn deficiency and Cd exposure strongly induced BcNRAMP1 transcription levels. Through transient expression of BcNRAMP1-GFP fusion protein in tobacco leaf epidermal cells, BcNRAMP1 was revealed as a plasma membrane protein. Expressing BcNRAMP1 in yeast enhanced yeast cells to absorb Mn, Cd, and iron (Fe). Overexpression of BcNRAMP1 in Arabidopsis wild-type and nramp1 mutant increased and complemented Mn and Cd transportation and accumulation, respectively. Using noninvasive microelectrode ion flux measurements, a direct evidence that BcNRAMP1 acts on Cd influx in Arabidopsis root cells was provided. The results of this study reveal that BcNRAMP1 functions as a NRAMP protein in planta, absorbing nutrient metal Mn and the toxic metal Cd.

Boron decreases cadmium influx into root cells of Capsicum annuum by altering cell wall components and plasmalemma permeability.

Large areas of soil are boron (B) deficient and contaminated with cadmium (Cd) in southern China. The aim of this study was to select the optimal B supply level and elucidate the underlying physiological and biochemical mechanisms to understand how B reduces Cd influx into root cells of hot pepper (Capsicum annuum). An experiment was conducted to investigate the changes in Cd accumulation with B supply. Hot pepper seedlings were grown in two nutrient solutions containing 0.05- and 0.2-mg Cd L-1 and supplied with six different B concentrations for 2 weeks. The other experiment was conducted to determine the Cd2+ flux into cells, cell wall components, antioxidative ability, and plasmalemma permeability of root tips of hot pepper exposed to 0.1-mg Cd L-1 in the presence and absence of B. The results showed that the optimal B concentration to promote plant growth and reduce Cd accumulation was 0.25 mg L-1. Moreover, B application significantly decreased Cd2+ influx into cells, increased the contents of lignin and pectin, enhanced the activities of antioxidant enzymes, reduced the production of reactive oxygen species, and decreased membrane peroxidation and permeability. Overall, boron in moderation can promote plant growth, maintain the normal structures and functions of the cell wall and membrane, and thus decrease Cd2+ influx into root cells and subsequently Cd translocation to shoots. Consequently, B is a reliable inhibitor of Cd uptake, and the functional and structural integrity of cell walls and membranes may have some relevance to reduced Cd uptake after B application.

Effect of exogenous silicon and methyl jasmonate on the alleviation of cadmium-induced phytotoxicity in tomato plants.

In the present study, a hydroponic experiment was performed to evaluate the effect of exogenous silicon (Si) and methyl jasmonate (MeJA) on the mitigation of Cd toxicity in tomato seedlings. The results revealed that Cd-stressed plants exhibited growth inhibition, increased lipid peroxidation, and impaired photosynthetic pigment accumulation. However, Si and MeJA applied alone or in combination significantly ameliorated the above-mentioned adverse effects induced by Cd. Among all treatments, Cd+Si+MeJA treatment elevated the dry mass of roots, stems, and leaves by 317.39%, 110.85%, and 119.71%, respectively. The chlorophyll a, chlorophyll b, and carotenoid contents in Cd+Si+MeJA-treated group were dramatically elevated (p < 0.05). Meanwhile, the malondialdehyde content in roots and shoots were reduced by 32.24% and 69.94%, respectively. The Si and MeJA applied separately or in combination also resulted in a prominent decrease of Cd influxes in tomato roots; therefore, a reduction of Cd content in tomato tissues were detected, and the Cd concentration in tomato roots were decreased by 27.19%, 25.18%, and 17.51% in Cd+Si, Cd+MeJA and Cd+Si+MeJA-treated plants, respectively. Moreover, in Cd+Si+MeJA-treated group, the percentage of Cd in cell wall fraction was enhanced while that in organelle fraction was decreased as compared with Cd-stressed plants. Collectively, our findings indicated that Si and MeJA application provide a beneficial role in enhancing Cd tolerance and reducing Cd uptake in tomato plants.

Graphene oxide decreases Cd concentration in rice seedlings but intensifies growth restriction

The extensive use of graphene oxide (GO) results in its inevitable entry into the environment, raising risks to the environment, especially the ecological risks when coexisting with other contaminants. Nevertheless, how GO affects the biological behavior of Cd in plants remains poorly understood. Here, we report that the transcript levels of Cd transporters, including OsIRT1, OsIRT2, OsNramp1, OsNramp5, and OsHMA2, were decreased by 56–96% in Cd-stressed rice seedlings with exposure to 400 mg L−1 GO compared with those without GO exposure. The in situ non-invasive microelectrodes test revealed that GO clearly reduced the net Cd influx of rice roots. Thus, GO exposure decreased the level of Cd in rice seedlings by approximately 60%, compared with the GO-free condition. However, the analyses of biomass, chlorophyll fluorescence parameters and Evans blue staining, indicated that GO had adverse effects on the robustness of plants under the Cd co-contaminated condition. Taken together, although GO reduced the accumulation of Cd in rice seedlings, it still negatively affected plant growth. Therefore, the positive and negative impacts of GO on crop production are of concern. Our findings provide new information for establishing a wider phytotoxicity evaluation system for the safe manufacture and use of GO.

Methane control of cadmium tolerance in alfalfa roots requires hydrogen sulfide

Hydrogen sulfide (H2S) is well known as a gaseous signal in response to heavy metal stress, while methane (CH4), the most prevalent greenhouse gas, confers cadmium (Cd) tolerance. In this report, the causal link between CH4 and H2S controlling Cd tolerance in alfalfa (Medicago sativa) plants was assessed. Our results observed that the administration of CH4 not only intensifies H2S metabolism, but also attenuates Cd-triggered growth inhibition in alfalfa seedlings, which were parallel to the alleviated roles in the redox imbalance and cell death in root tissues. Above results were not observed in roots after the removal of endogenous H2S, either in the presence of either hypotaurine (HT; a H2S scavenger) or DL-propargylglycine (PAG; a H2S biosynthesis inhibitor). Using in situ noninvasive microtest technology (NMT) and inductively coupled plasma mass spectroscopy (ICP-MS), subsequent results confirmed the participation of H2S in CH4-inhibited Cd influx and accumulation in roots, which could be explained by reestablishing glutathione (GSH) pool (reduced/oxidized GSH and homoglutathione) homeostasis and promoting antioxidant defence. Overall, our results clearly revealed that H2S operates downstream of CH4 enhancing tolerance against Cd stress, which are significant for both fundamental and applied plant biology.

Physiological response of Arizona cypress to Cd-contaminated soil inoculated with arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria

Increasing some industrial activities has been constantly contaminated the soil with different toxic metals leading to serious environmental concerns. However, some beneficial soil microorganisms would be useful tools for reducing toxic effects of heavy metals without producing toxic residuals. Therefore, present study was conducted to evaluate potential role of Pseudomonas fluorescens and arbuscular mycorrhizal fungi (AMF) in reducing cadmium toxicity in the Arizona cypress seedlings both individually and in combination. Factorial experiment was conducted based on a completely randomized design with three factors comprising AMF inoculation (Rhizophagus irregularis or Funneliformis mosseae or a mixture of both species, and non-inoculated controls), bacterial inoculation (P. fluorescens or Non-inoculated), and five levels of Cd (0, 5, 10, 15, and 20 mg kg−1) using three replications. Results of this study showed that, Arizona cypress growth decreased when exposed to high levels of Cd contamination. However, co-inoculation plants with all mycorrhizae and P. fluorescens grown under the highest levels of Cd contamination, showed increased photosynthetic pigments. Furthermore, AMF and P. fluorescens increased ascorbate peroxidase activity, which is responsible for the elimination of H2O2 when plants exposed to Cd stress. Moreover, high levels of Cd contamination negatively influenced phosphorus uptake by the plants, especially in control plants. Moreover, in seedlings inoculated with mycorrhizal fungi, accumulation of Cd was found to be less, apparently as a result of AMF modification of the influx of Cd into the host plants, as confirmed by reduced hydrogen peroxide and enhanced antioxidant enzyme activities compared to the control plants. AMF treatments increased glomalin related soil proteins concentration compared to the control plants. Also, the highest amount of glomalin production was observed in two species of Mix and FM. In conclusion, either co-inoculation with AMF and P. fluorescens or individual inoculation with AMF could potentially ameliorate harmful effects of Cd on Arizona cypress growth. .

A comparative study of the factors affecting uptake and distribution of Cd with Ni in barley

Food crops often accumulate heavy metals above the recommended limits. Cadmium (Cd) is particularly harmful in terms of its potential dangers to human health. The effects of nutrient status and cation competition on Cd uptake and distribution in barley were investigated to analyse the main route for Cd entry into the plants. Cd uptake into whole plants was measured by radiotracer studies and elemental analysis using environmentally relevant concentrations. The nutrient status of the plants was altered by growing them hydroponically in micronutrient-deficient conditions (-Fe, -Mn, or -Zn). Fe and Zn were found to have a large effect on the uptake of Cd both via deficiencies and by the competition for uptake. However, Mn was found to have no effect on the uptake of Cd either via deficiency or by the competition for uptake. This strongly suggests that the main route for Cd uptake into the roots is via Fe and Zn transporters. The inhibition of Cd influx only by FeII (but not by FeIII) suggests that Cd uptake into the root occurs through divalent cation transporters. Since Cd is a non-essential metal in plants, the transport characteristics were compared with those of an essential micronutrient, Ni. At the same external concentration, more than twice as much Cd was absorbed as Ni in all of the different nutrient conditions. Ni translocation to the shoot was much lower than for Cd. The comparison of two metals showed some similarities in the root uptake processes but not in the shoot translocation.