Cd In Earthworms Research Articles

Co-exposure of decabromodiphenyl ethane and cadmium increases toxicity to earthworms: Enrichment, oxidative stress, damage and molecular binding mechanisms

The increase of electronic waste worldwide has resulted in the exacerbation of combined decabromodiphenyl ethane (DBDPE) and cadmium (Cd) pollution in soil, posing a serious threat to the safety of soil organisms. However, whether combined exposure increases toxicity remains unclear. Therefore, this study primarily investigated the toxic effects of DBDPE and Cd on earthworms at the individual, tissue, and cellular levels under single and combined exposure. The results showed that the combined exposure significantly increased the enrichment of Cd in earthworms by 50.32–90.42 %. The toxicity to earthworms increased with co-exposure, primarily resulting in enhanced oxidative stress, inhibition of growth and reproduction, intensified intestinal and epidermal damage, and amplified coelomocyte apoptosis. PLS-PM analysis revealed a significant and direct relationship between the accumulation of target pollutants in earthworms and oxidative stress, damage, as well as growth and reproduction of earthworms. Furthermore, IBR analysis indicated that SOD and POD were sensitive biomarkers in earthworms. Molecular docking elucidated that DBDPE and Cd induced oxidative stress responses in earthworms through the alteration of the conformation of the two enzymes. This study enhances understanding of the mechanisms behind the toxicity of combined pollution and provides important insights for assessing e-waste contaminated soils.

Influence of aged and pristine polyethylene microplastics on bioavailability of three heavy metals in soil: Toxic effects to earthworms (Eisenia fetida)

Virgin microplastics (MPs) would undergo aging process when entering environment, the adsorption capability of pollutants onto MPs may change during the aging process. To better understand the influence of aged polyethylene microplastics (PE-MP) on the bioavailability of three heavy metals (Zn, Pb, and Cd) in soil, hydrogen peroxide exposure (3% H2O2) and ultraviolet irradiation methods were employed to simulate the aging process. After aging process, different amount (0.1%, 1%, 10%) of PE-MP (pristine or aged) was added into soil to assess the ability of soil (containing PE-MP) adsorbing heavy metal. Moreover, different amount (0.01%, 0.1%, 1%) of PE-MP (pristine or aged) was added into soil to cultivate the earthworms to assess the impact of PE-MP on bioavailability of three heavy metals. Results indicated that the aged and virgin PE-MP had similar capability to adsorb heavy metal, the adsorption ability of Zn2+, Pb2+, and Cd2+ to pristine PE-MP were 2.42, 7.47, and 7.76 mg/g, respectively. The concentration of Zn or Pb in earthworms in treatments of metal +1% PE-MP was slightly higher than that in single metal (Zn or Pb) treatment, moreover, the concentration of Cd in earthworms in treatment of Cd + 1% PE-MP was significantly (p < 0.05) higher than that in single Cd treatment, exhibiting that 1% of PE-MP enhanced the bioavailability of heavy metals in soil. However, heavy metal concentrations in earthworms in treatments of metal + pristine PE-MP showed insignificant (p > 0.05) difference with those in treatments of metal + aged PE-MP, indicating that the aging process in this study did not change the environmental influence of PE-MP on heavy metals bioavailability. Superoxide dismutase activity, reactive oxygen species level, malondialdehyde content, and related gene expression in earthworms showed that PE-MP and heavy metals would bring toxic synergy to earthworms, therefore, the influence of MPs should be comprehensively considered when determining the environmental risk of heavy metals in soil.

Novel bioavailability-based risk assessment of Cd in earthworms and leeches utilizing in vitro digestion/Caco-2 and MDCK cells.

In the present study, the oral bioavailability of cadmium (Cd) in earthworms and leeches was investigated through in vitro physiologically based extraction test (PBET) digestion/Caco2 and MDKC cell models. We are the first to create an innovative assessment strategy which has capacity to offer a more precise evaluation of Cd-associated health risks in traditional animal medicines (TAMs), by combinational usage of bioavailable Cd levels, the duration and frequency of the exposure to TAMs obtained by questionnaire data, as well as safety factor of TAMs. Our data showed that the percentage of bioavailability for Caco-2 cells in earthworms and leeches ranged from 3.29 to 14.17% and 4.32 to 12.61%, respectively. The percentage of bioavailability of MDCK cells in earthworms and leeches ranged from 4.83 to 15.74% and 6.53 to 15.04%, respectively. After adjusting by the bioavailability of Cd to target hazard quotient (THQ), excitingly, our findings manifested that the health risks induced by the ingestion of earthworms and leeches were acceptable in the clinic. Our key findings suggest that bioavailability characterization cannot be ruled out and health risks should be assessed on the basis of the bioavailable Cd levels rather than total levels. Our novel strategy provides insight into the bio-accumulation of Cd in organisms as well as a more realistic and accurate assessment of Cd-associated health risks in TAMs, with the main purpose of improving public health by scientifically using TAMs.

Bioremediation of Cd-spiked soil using earthworms (Eisenia fetida): Enhancement with biochar and Bacillus megatherium application

In this study, we evaluated the influence of biochar and Bacillus megatherium on Cd removal from artificially contaminated soils using earthworms (Eisenia fetida). Within a 35-days remediation period, over 30% of Cd was removed by earthworms from the contaminated soil (with Cd at ∼ 2.5 mg kg−1), and both additives facilitated Cd removal. Additionally, over 22% reduction in the extractable Cd contents was also achieved by earthworms. Cd accumulated in earthworms steadily increased through remediation, and the accumulated Cd decreased in the order of earthworm + biochar (T3) > earthworm + Bacillus megatherium (T4) > earthworm alone (T2). The bioaccumulation factors (BCF) were above 1, indicating the enrichment of Cd in earthworms, and there were higher BCF for both T4 (944%) and T3 (845%). The ingestion of metal-bonded biochar particle and the elevated Cd mobility would be the main reason for the enhanced Cd-remediation by earthworms under T3 and T4, respectively. Through remediation, microbiota communities in both, soil and earthworm guts, demonstrated high similarity, while a lower level of bacterial abundance was observed in earthworm guts compared with that in soils. Eventually, soils became more fertile and demonstrated higher enzyme activities after remediation. Therefore, we concluded that earthworm, alone or combined with biochar or Bacillus megatherium could be an alternative method for Cd-contaminated soil remediation.

Assessment of the immobilization effectiveness of several amendments on a cadmium-contaminated soil using Eisenia fetida.

Proper protocols for assessing the remediation effectiveness of contaminated soils are an important part of remediation projects. In the present study, the residual immobilization effectiveness of hydrated lime (L), hydroxyapatite (H), biochar (B) and organic fertilizer (F) alone and in combination was assessed by Eisenia fetida. The results showed that the application of amendments had no significant effect on the death rate and average fresh weight loss of earthworms. The earthworm Cd concentration increased with prolonged exposure time, however, the significant immobilization efficacy of amendments observed on the 7th day nearly disappeared after 28 days of exposure. The immobilization efficiencies, estimated by the earthworms internal Cd concentration, of L, H and B on the 7th day were 38.6%, 37.8% and 20.7%, respectively. These values decreased to 4.9%, 19.8% and 15.1%, respectively, on the 28th day. The detoxification effect of amendments was confirmed by the Cd subcellular fractionation in earthworms with lower proportions of Cd distributed in the metal-sensitive fractions in L, H and B treatments. The level of oxidative stress response of earthworms increased with exposure duration and amendments alleviated the oxidative damage induced by Cd to the earthworms. In addition, the pH and CaCl2-Cd in soils were both increased due to earthworm life activities and gut-related ingestion. In summary, the assessment of immobilization effectiveness of heavy metal-contaminated soils using Eisenia fetida was time-dependent. The immobilization efficacy of L and H performed better than B and F on the 7th day, while H and B performed better than L and F on the 28th day. Accordingly, the short-term earthworm exposure experiment (7 days) was recommended to be an alternative approach to time-consuming plant bioassays in assessment of reduced phytoavailability in chemical immobilization remediation. But the impact of earthworms on the immobilization effect of amendments needs to be considered in practical remediation.

Trace Elements in Surface Soils and Megascolecidae Earthworms in Urban Forests Within Four Land-Uses Around Poughkeepsie, New York, USA.

Amynthas agrestis and Metaphire hilgendorfi are being distributed across North America with unknown ecosystem impacts. Forest soils in urban areas sequester trace elements and earthworms may be bioaccumulating them. This study examined Cd, Cr, Co, Cu, Mn, Ni, Pb, V, and Zn in soils and earthworm tissues at 28 urban forest sites in and surrounding Poughkeepsie, NY, USA. Megascolecidae were present at 22 sites with means of 12 to 27 individuals m-2 and 4 to 12dryweightgm-2. Urban forest soils within commercial uses had Mn, Pb, and Zn concentrations higher than within residential and agricultural uses. Earthworm trace element concentrations were poorly predicted by their respective soil concentrations, except for Pb. Urban forests in commercial uses and land-preserves, earthworm Cd and Pb concentrations were at or above concentrations known to negatively impact small mammal and bird health ( > 10mgkg-1) with Co and V approaching toxic concentrations.

Does ecotype matter? The influence of ecophysiology on benzo[a]pyrene and cadmium accumulation and distribution in earthworms

Benzo[a]pyrene (BaP) and cadmium (Cd) are soil pollutants that persist in the environment and impacting soil health. Earthworms are selective consumers, yet little is known about how their feeding and burrowing habits translate into species-specific differences in pollution accumulation and distribution. Here, we exposed three ecophysiologically distinct earthworm species, Eisenia fetida (epigeic), Pheretima guillelmi (endogeic), and Metaphire guillelmi (anecic) to different concentrations (0, 1, 10, 30, 60, 100, 300, 500 mg/kg) of BaP or Cd in natural fluvo–aquic soil for 14 days. BaP and Cd accumulation and distribution patterns were analyzed at the individual and organ levels. The results showed that the adsorption behavior of BaP or Cd in earthworms and the organs fit the Langmuir adsorption model well (R2 > 0.8, p < 0.001). E. fetida and M. guillelmi accumulated more BaP than Cd, with the respectively higher predicted maximum internal concentration (Cmax) of BaP (1946.27 ± 306.29 mg/kg, 2046.61 ± 90.64 mg/kg) and the lower Cmax of Cd (279.75 ± 49.57 mg/kg, 318.11 ± 60.64 mg/kg), while P. guillelmi showed the opposite trend with 927.10 mg/kg of Cd Cmax and 358.82 ± 68.35 mg/kg of BaP Cmax. The low mobility of endogeic worms may reduce their BaP accumulation, and lead to a lower BaP bioaccumulation factor (BAF) than that observed for the other two earthworms; P. guillelmi had a BAF of 8.64 ± 1.79, which was far less than that of E. fetida (106.93 ± 11.84) and M. guillelmi (350.16 ± 67.15). Whereas the higher Cd accumulation in P. guillelmi may be due to their highly efficient geophagous feeding strategy, at the same time the highest Cd BAF achieved (e.g. 203.54 ± 19.96 under 1 mg/kg Cd exposure). BaP distributed mainly in the body walls of all three earthworms (average 60.78%), as its high hydrophobicity increased its dermal uptake. More BaP than Cd accumulated in the reproductive organs, and Cmax of BaP in E. fetida, P. guillelmi, and M. guillelmi in the reproductive organs was 4031.08 ± 1237.38 mg/kg, 490.76 ± 79.88 mg/kg, and 3675.24 ± 794.68 mg/kg, respectively. However, Cd was more abundant in the gizzard and gut, as its hydrophilic nature meant Cd was mainly ingested orally, and the Cmax of Cd in E. fetida, P. guillelmi, and M. guillelmi in the gizzard and gut, respectively, was 452.43 ± 48.33 and 360.83 ± 44.36 mg/kg, 996.03 and 809.11 mg/kg, and 93.37 and 109.19 mg/kg. By contrast, for E. fetida, more Cd was distributed in the body wall (average 50.16%), possibly due to its high affinity for this organ (the average logKL for the body wall was −3.60) predicted by the Langmuir adsorption model, also the worm ingested less soil. These data suggest that ecotype influences the accumulation and distribution of pollutants in earthworms, as their ecophysiological properties (e.g., motility, food choices, and feeding efficiency) affect their exposure to and ingestion of pollutants. The distinct chemical properties of BaP and Cd also appear to affect their accumulation and distribution in earthworms. These factors should be considered when using earthworms as bioindicators in environmental risk assessments.

Toxicokinetics of Zn and Cd in the earthworm Eisenia andrei exposed to metal-contaminated soils under different combinations of air temperature and soil moisture content

This study evaluated how different combinations of air temperature (20 °C and 25 °C) and soil moisture content (50% and 30% of the soil water holding capacity, WHC), reflecting realistic climate change scenarios, affect the bioaccumulation kinetics of Zn and Cd in the earthworm Eisenia andrei. Earthworms were exposed for 21 d to two metal-contaminated soils (uptake phase), followed by 21 d incubation in non-contaminated soil (elimination phase). Body Zn and Cd concentrations were checked in time and metal uptake (k1) and elimination (k2) rate constants determined; metal bioaccumulation factor (BAF) was calculated as k1/k2. Earthworms showed extremely fast uptake and elimination of Zn, regardless of the exposure level. Climate conditions had no major impacts on the bioaccumulation kinetics of Zn, although a tendency towards lower k1 and k2 values was observed at 25 °C + 30% WHC. Earthworm Cd concentrations gradually increased with time upon exposure to metal-contaminated soils, especially at 50% WHC, and remained constant or slowly decreased following transfer to non-contaminated soil. Different combinations of air temperature and soil moisture content changed the bioaccumulation kinetics of Cd, leading to higher k1 and k2 values for earthworms incubated at 25 °C + 50% WHC and slower Cd kinetics at 25 °C + 30% WHC. This resulted in greater BAFs for Cd at warmer and drier environments which could imply higher toxicity risks but also of transfer of Cd within the food chain under the current global warming perspective.

Biomonitoring of chemicals in biota of two wetland protected areas exposed to different levels of environmental impact: results of the "PREVIENI" project.

The PREVIENI project (funded by the Ministry of Environment) investigated the exposure to endocrine disrupters in samples of human population and environmental biota in Italy. The environmental biomonitoring considered two Italian WWF Oasis, with the aim to compare the presence and effects of endocrine disruptors in organisms from two protected natural areas, respectively, upstream and downstream a chemical emission site. Chemical analysis of pollutants' tissue levels was made on tissues from earthworm, barbell, trout, and coot, selected as bioindicator organisms. The contaminants considered were as follows: the perfluorinated compounds perfuoroctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA), polychlorinated biphenyls (PCBs 58 congeners), polybrominated diphenyl ethers (PBDEs, 13 congeners), polycyclic aromatic hydrocarbons (PAHs, 16 compounds), toxic trace elements, the phthalate di-2-ethylexyl phthalate (DEHP) and its primary metabolite, bisphenol A, synthetic musk compounds (musk xylene, musk ketone, tonalide, and galaxolide), and p-nonylphenol. The analyses showed low concentrations of most pollutants in all species from both areas, compared to available literature; noticeable exceptions were the increases of DEHP's primary metabolite, PBDE, PAHs, Hg, and Pb in barbells, and of PCB and Cd in earthworms from the downstream area. The results showed the presence of endocrine disruptors, including those considered as "non-persistent," in bioindicators from protected areas, albeit at low levels. The results provide a contribution to the evaluation of reference values in biota from Mediterranean Europe and support the relevance of monitoring exposure to pollutants, in particular for freshwater environment, also in protected areas.

Metal/metalloid (As, Cd and Zn) bioaccumulation in the earthworm Eisenia andrei under different scenarios of climate change

This study aimed at assessing the effects of global warming (increasing air temperature and decreasing soil moisture content) on the bioaccumulation kinetics of As, Cd and Zn in the earthworm Eisenia andrei in two polluted soils (mine tailing and watercourse soil). Earthworms were exposed for up to 21 d under four climate conditions: 20 °C + 50% soil water holding capacity (WHC) (standard conditions), 20 °C + 30% WHC, 25 °C + 50% WHC and 25 °C + 30% WHC. Porewater metal/metalloid availability did not change in the mine tailing soil after the incubation period under the different climate conditions tested. However, in the watercourse soil, porewater Cd concentrations decreased from ∼63 to ∼32–41 μg L−1 after 21 d and Zn concentrations from ∼3761 to ∼1613–2170 μg L−1, especially at 20 °C and 50% WHC. In both soils, As and Zn showed similar bioaccumulation patterns in the earthworms, without major differences among climate conditions. Earthworm concentrations peaked after 1–3 d of exposure (in μg g−1 dry weight: As∼32.5–108, Zn∼704–1172) and then remained constant (typical pattern of essential elements even for As). For Cd the bioaccumulation pattern changed when changing the climate conditions. Under standard conditions, earthworm Cd concentrations increased to ∼12.6–18.5 μg g−1 dry weight without reaching equilibrium (typical pattern of non-essential elements). However when increasing temperature and/or decreasing soil moisture content the bioaccumulation pattern changed towards that more typical of essential elements due to increased Cd elimination rates (from ∼0.11 to ∼0.24–1.27 d−1 in the mine tailing soil, from ∼0.07 to ∼0.11–0.35 d−1 in the watercourse soil) and faster achievement of a steady state. This study shows that metal/metalloid bioaccumulation pattern in earthworms may change dependent on climate conditions.

Testing Single and Combinations of Amendments for Stabilization of Metals in Contrasting Extremely Contaminated Soils

Metals can be stabilized by soil amendments that increase metals adsorption or alter their chemical forms. Such treatments may limit the risk related to the contamination through reduction of metal transfer to the food chain (reduction of metal uptake by plants and its availability to soil organisms) and metals migration within the environment. There is a need for experiments comparing various soil amendments available at reasonable amounts under similar environmental conditions. The other question is whether all components of soil environment or soil functions are similarly protected after remediation treatment. We conducted a series of pot studies to test some traditional and novel amendments and their combinations. The treatments were tested for several highly Zn/Cd/Pb contaminated soils. Among traditional amendments composts were the most effective - they ensured plant growth, increased soil microbial activity, reduced Cd in earthworms, reduced Pb bioaccessibility and increased share of unavailable forms of Cd and Pb.

Effect of Complexation on the Accumulation and Elimination Kinetics of Cadmium and Ciprofloxacin in the Earthworm Eisenia fetida

Land application of solid wastes leads to the accumulation of both metals and antimicrobials in soils. To understand the effects of metal and antibiotic interaction on their accumulation by the earthworm Eisenia fetida, uptake and elimination kinetics and subcellular distribution of cadmium (Cd) and ciprofloxacin (CIP) were determined. The kinetics was accurately described by a one-compartment first-order kinetic model. Bioaccumulation kinetics and subcellular distribution of CIP were not affected by Cd addition. However, Cd exhibited different metabolic and subcellular distribution patterns. With CIP, Cd uptake flux and elimination rate constants were about 2.2 and 9.8 times, respectively, those without CIP. In the presence of CIP, Cd redistributed from fractions D (associated with granules) and E (associated with tissue fragments and cell membranes) to fraction C (associated with cytosol). Without CIP, Cd in fraction C could not be excreted, whereas with CIP, Cd in fraction C was significantly excreted, and the excretion rate constant was consistent with that of CIP. A good relationship was found between CIP and Cd in earthworms during uptake and elimination periods (p < 0.01). Our results indicated that the Cd-CIP complex may be taken up, stored, and eliminated by earthworms.

Bioaccumulation and single and joint toxicities of penta-BDE and cadmium to earthworms (Eisenia fetida) exposed to spiked soils

Bioaccumulation of penta-BDE (DE-71) in earthworms (Eisenia fetida) and the induced toxicities on the growth and reproduction of earthworms were investigated. All the major congeners in DE-71 could be bioaccumulated in earthworms and the concentration found in earthworms correlated to the spiked concentration in soil. DE-71 might inhibit the growth and reproduction of cocoons and juveniles of earthworms. The toxicities were dose dependent and increased with exposure time. Exposing earthworms to combination of DE-71 and Cd resulted in enhanced mortality and reduction of cocoons or juveniles in a synergistic mode. The presence of DE-71 may affect the relocation of Cd in earthworms. When the earthworms were exposed to Cd alone, Cd up-taken by earthworms was mainly partitioned in the cytosolic fraction. While DE-71 was present, Cd in the cytosolic fraction decreased significantly. It is perhaps that DE-71 inhibits the synthesis of matallothioneins, and then reduces the detoxification ability of earthworms. This is the first report about the toxicity of PBDEs to earthworms. The result would be useful for ecological risk assessment of PBDEs in terrestrial ecosystem.

Uptake pathways and toxicity of Cd and Zn in the earthworm Eisenia fetida

The uptake of Cd and Zn by the earthworm Eisenia fetida was determined at varying Ca concentrations and with pre-exposure to different metabolic inhibitors in simulated soil solutions over a 48-h period. The presence of Ca in the solution had complex actions on Cd uptake. At a low Cd concentration of 0.1 μM, Ca (0.1–1 mM) slightly but significantly stimulated Cd uptake, whereas it inhibited Cd uptake at a higher Cd level (10 μM). Pre-exposure to a Ca-channel blocker (Lanthanum) inhibited Cd uptake over a relatively wide range of Cd concentrations, but not Zn uptake, suggesting that the uptake of Zn was not exerted at a Ca channel. N-ethylmaleimide, which specifically binds to sulfhydryl groups, inhibited Zn uptake at both 0.1 and 10 μM, implying that the transport of Zn is carrier-mediated by proteins or other SH-containing compounds. The present study provides evidence that the mechanisms of Cd and Zn uptake in earthworms are pharmacologically different, although both metals have similar geochemical and environmental properties. After 24 h pre-exposure to a sublethal concentration (1.0 μM) of Cd, Zn toxicity for E. fetida was significantly reduced with 48-h LC 50 values (with 95% confidence interval) increasing from 145 (105–201) to 316 (212–470) μM Zn. Pre-exposure to Zn (1.0 μM) did not, however, affect Cd toxicity. Pre-exposure to Cd significantly changed the subcellular Zn distribution, with a decreasing fraction of Zn associated with Fraction B (associated with granules and cell membranes), which is believed to be most indicative of toxic pressure and an increased fraction associated with Fraction G (associated with cytosol). This most likely explains the observed Zn tolerance of E. fetida after low level Cd pre-exposure. These results help to understand the uptake mechanism and interactions of Zn and Cd in earthworms.

Method for determining toxicologically relevant cadmium residues in the earthworm Eisenia fetida.

We investigated a method to isolate toxicologically relevant Cd in earthworms (Eisenia fetida) exposed in a 14-d Cd bioaccumulation study. A procedure involving acid insoluble ash (AIA) content was combined with homogenization and centrifugation techniques to divide total earthworm Cd burdens into supernatant (metallothionein-bound), pellet (toxicologically active), and soil-associated Cd fractions. Whereas the supernatant fraction of the earthworm digests increased linearly throughout the exposure period (from approximately 0 to 3.59 mmol/kg), the pellet fraction reached a steady-state concentration (95% CI) of 1.2 (0.9-1.4) mmol/kg, suggesting the ability of the metallothionein detoxification system to sequester incoming Cd. The AIA method was useful for correcting earthworm Cd concentrations for ingested soil-associated Cd and observing soil ingestion, which was suppressed in Cd-spiked artificial soil (1.06 (0.57-1.55)%) compared to controls (17.25 (14.36-20.15)%). These methods may be useful in investigating soil ingestion and Cd uptake and detoxification in earthworms.

Earthworms as biological monitors of cadmium, copper, lead and zinc in metalliferous soils

Earthworms ( Lumbricus rebellus and Dendrodrilus rubidus) were sampled from one uncontaminated and fifteen metal-contaminated sites. Significant positive correlations were found between the earthworm and ‘total’ (conc. nitric acid-extractable) soil Cd, Cu, Pb and Zn concentrations (data log 10 transformed). The relationships were linear, and the accumulation patterns for both species were similar when a single metal was considered, even though there were species difference in mean metal concentrations. Generally, the earthworm Cd concentration exceeded that of the soil; by contrast, the worm Pb concentration was lower than the soil Pb concentration in all but one (acidic, low soil Ca) site. Our observations suggest that Cu and Zn accumulation may be physiologically regulated by both species. Total-soil Cd explained 82–86% of the variability (V 2) in earthworm Cd concentration; 52–58% of worm Pb and worm Zn concentrations were explained by the total-soil concentrations of the respective metals. Total-soil Cu explained only 11–32% of the worm Cu concentration. The effect of soil pH, total Ca concentration, cation-exchange capacity (CEC) and organic carbon on metal accumulation by L. rubellus and D. rubidus was investigated by multiple regression analysis. Soil pH (coupled with CEC) and soil Ca had a major influence on Pb accumulation (V 2 of worm Pb increased to 77–83%), and there was some evidence that Cd accumulation may be suppressed in extremely organic soils. The edaphic factors investigated had no effect on Cu or Zn accumulation by earthworms. In the context of biomonitoring, it is proposed that earthworms have a potential in a dual role: (1) as ‘quantitative’ monitors of total-soil metal concentrations (as shown for Cd); and (2) as estimators of ‘ecologically significant’ soil metal, integrating the effects of edaphic factors (as shown for Pb).

Metal Concentrations in Earthworms From Sewage Sludge‐Amended Soils at a Strip Mine Reclamation Site

AbstractA 3‐yr study of earthworms was initiated in selected minesoil and nonmined fields at a Fulton County, IL land reclamation site. The purpose of this research was to determine the effect of the land application of anaerobically digested sewage sludge, used to reclaim the site, on heavy metal accumulations in earthworms.Two species of earthworms, Lumbricus terrestris and Aporrectodea tuberculata, were identified in the sludge‐amended and nonamended, nonmined fields sampled. Only A. tuberculata was found in the sludge‐amended and nonamended minesoil fields sampled.Earthworm metal concentrations generally increased with time in all the sampled fields. The decreasing order of metal accumulation by earthworms in all sludge‐amended fields sampled was Cu &gt; Cd &gt; Ni &gt; Cr &gt; Pb &gt; Zn. Sewage sludge applications to fields on both land types resulted in significant accumulations of Cd, Cu, and Zn. Land type (minesoil vs. nonmined) significantly affected earthworm Zn concentrations, with levels being higher in all nonmined fields sampled. Earthworm Cd and Cu accumulations in all fields sampled were significantly related to the current amounts of sludge‐applied metals, the amount applied since the previous sampling. Concentrations of Ni, Cr, and Pb in earthworms were not significantly related to sewage sludge applications during the 1975 to 1977 sampling period. The higher Cd and Cu concentrations in earthworms from sludge‐amended fields may pose a potential hazard to predators.