Cavitation Bubble Cloud Research Articles

Safety and quality of life assessment at 6 months follow-up of the valvosoft pivotal study

Abstract Background Calcific Aortic stenosis (CAS) is currently the most common heart valve disease. Patients suffering from severe CAS are treated with invasive surgeries based on valve replacement. Symptoms heavily impact quality of life, including chest pain, shortness of breath, dizziness, fatigue, and major risks as heart failure or sudden death, and daily activities might also be strongly impacted. Purpose A prospective, multi-centre, single-arm, pivotal study (NCT05235568) was initiated in 2021 to treat severe CAS patients with Valvosoft, a medical device based on Non-Invasive Ultrasound Therapy (NIUT) intended to treat CAS in patients not eligible for immediate aortic valve replacement (SAVR/TAVR) or refusing such a treatment, to confirm safety and performance at 1-, 3-, 6- and 12 months. We present here the safety and quality of life results at 6M follow-up. Methods Valvosoft NIUT procedure is based on a trans-thoracic delivery of focused, high intensity short therapeutic ultrasound pulses, to precisely generate a cavitation bubble cloud on the aortic valve. Echocardiographic imaging enables to monitor the valve in real-time while cavitation causes both non-thermal tissue softening and calcification fractionation of the diseased valve. 60 planned subjects, suffering from severe calcific aortic stenosis, not recommended for aortic valve replacement or refusing such a procedure, were enrolled in 11 centres in Europe. Patients were awake or lightly sedated, NIUT was delivered during up to 70 minutes per patient per procedure. DSMB and CEC were installed to monitor safety, performance and effectiveness of Valvosoft device, and safety related to participation in the clinical investigation. Results Sixty high-risk patients advanced in age (85.2 ± 9.1) with multiple severe comorbidities were included. Primary safety endpoint (less than 25% MACE) was met at 30D (p=0.002). Furthermore patients experienced an overall quality of life improved. 6 minutes’ walk test increases from baseline towards 6 months with 10 %. At 6M follow-up, no stroke was reported, an average KCCQ score improvement of 17% was noticed, and NYHA class improved or stabilized for 78% of patients. The calcium score or volume on the aortic did not change pre- and post-procedure. Conclusion Valvosoft NIUT safety and quality of life improvement are proven at 6MFU, and the technology is presented as potentially disruptive, in the treatment of severe calcific aortic stenosis.

Effect of Acoustically Responsive Droplet-based Low-intensity Histotripsy on Canine Prostate

ObjectiveLow-intensity histotripsy (LIH) is a novel and safe technique for tissue ablation. This study aimed to explore the effects of LIH on canine prostate tissue and identify the degree of acute injury to the gland. MethodsWe constructed and evaluated two types of acoustically responsive droplet (ARD) emulsions using either perfluoropentane (PFP) with a lipid shell or perfluoromethyl-cyclopentane (PFMCP) with lauromacrogol (L) injection. Twenty beagles were assigned to four experimental groups: ultrasound (US) + PFP (n = 6), US + PFMCP-L (n = 6), PFMCP-L (n = 5) and PFP (n = 3). The ARDs were injected transcutaneously and transabdominally into normal canine prostates under US-guided imaging. Subsequently, focused therapeutic US was employed to induce acoustic droplet vaporization and bubble cloud cavitation. The mechanical damage to canine prostate tissue was evaluated using gross and histological examination. ResultsGross specimens showed that the injured area was dark brown. Hematoxylin and eosin-stained tissue sections of the damage zone showed significant cavity formation and interstitial edema. The total tissue damage scores in the US + PFP group were compared to those of the other three experimental groups. No statistically significant differences were observed in the extent of tissue damage and total scores among the US + PFMCP-L, PFMCP-L and PFP groups. ConclusionWe achieved significant mechanical tissue damage in the canine prostate using PFP ARD-based LIH that proved to be superior to that using PFMCP ARDs with LIH.

Non-Invasive Ultrasound Therapy for Severe Aortic Stenosis: Early Effects on the Valve, Ventricle, and Cardiac Biomarkers (A Case Series).

Background: Transcatheter aortic valve replacement (TAVR) was developed for inoperable patients with severe aortic stenosis. However, despite TAVR advancements, some patients remain untreated due to complex comorbidities, necessitating less-invasive approaches. Non-invasive ultrasound therapy (NIUT), a new treatment modality, has the potential to address this treatment gap, delivering short ultrasound pulses that create cavitation bubble clouds, aimed at softening embedded calcification in stiffened valve tissue. Methods: In the prospective Valvosoft® Serbian first-in-human study, we assessed the safety and efficacy of NIUT and its impact on aortic valve hemodynamics, on the left ventricle, and on systemic inflammation in patients with severe symptomatic aortic stenosis not eligible for TAVR or surgery. Results: Ten patients were included. Significant improvements were observed in hemodynamic parameters from baseline to one month, including a 39% increase in the aortic valve area (from 0.5 cm2 to 0.7 cm2, p = 0.001) and a 23% decrease in the mean transvalvular gradient (from 54 mmHg to 38 mmHg, p = 0.01). Additionally, left ventricular global longitudinal strain significantly rose, while global wasted work significantly declined at one month. A dose-response relationship was observed between treatment parameters (peak acoustic power, intensity spatial-peak pulse-average, and mean acoustic energy) and hemodynamic outcomes. NIUT was safely applied, with no clinically relevant changes in high-sensitivity troponin T or C-reactive protein and with a numerical, but not statistically significant, reduction in brain natriuretic peptide (from 471 pg/mL at baseline to 251 pg/mL at one month). Conclusions: This first-in-human study demonstrates that NIUT is safe and confers statistically significant hemodynamic benefits both on the valve and ventricle.

Timber DNA release using focused ultrasound extraction (FUSE) for genetic species identification

The use of genetic data for timber species and population assignment is a powerful tool for combating the illegal timber trade, but the challenges of extracting DNA from timber have prevented the routine use of genetics as a supply chain management tool. To overcome these challenges, we explored the feasibility of focused ultrasound extraction (FUSE) for rapid DNA release from timber. Using high-pressure ultrasound pulses, FUSE generates a cavitation bubble cloud that disintegrates samples into acellular debris, resulting in the mechanical release of DNA. In this work, FUSE was applied to white oak (Quercus alba) timber shavings to test the feasibility of using FUSE for timber DNA extraction for the first time. Results showed that FUSE processing disintegrated the tissue samples and released significant quantities of DNA. After five minutes of tissue processing DNA quantities of 0.21 ± 0.02 ng/mg, 0.99 ± 0.32 ng/mg, and 0.14 ± 0.01 ng/mg, were released from medium, coarse, and combination shaving groups, respectively. Amplification and sequencing of regions within the matK and rbcL chloroplast genes confirmed that the quality of DNA prepared with FUSE was suitable for PCR and short-read sequencing applications. Overall, these results show that FUSE can serve as a DNA sample preparation method capable of releasing high-quality DNA from timber in a fraction of the time required by conventional extraction methods. Based on the improved efficiency of DNA release with FUSE, ongoing work aims to develop this technology into portable systems that can be used to rapidly prepare timber samples for genetic species identification.

Histotripsy and Catheter-Directed Lytic: Efficacy in Highly Retracted Porcine Clots In Vitro

ObjectiveStandard treatment for deep vein thrombosis (DVT) involves catheter-directed anticoagulants or thrombolytics, but the chronic thrombi present in many DVT cases are often resistant to this therapy. Histotripsy has been found to be a promising adjuvant treatment, using the mechanical action of cavitating bubble clouds to enhance thrombolytic activity. The objective of this study was to determine if histotripsy enhanced recombinant tissue plasminogen activator (rt-PA) thrombolysis in highly retracted porcine clots in vitro in a flow model of occlusive DVT. MethodsHighly retracted porcine whole blood clots were treated for 1 h with either catheter-directed saline (negative control), rt-PA (lytic control), histotripsy, DEFINITY and histotripsy or the combination of rt-PA and histotripsy with or without DEFINITY. Five-cycle, 1.5 MHz histotripsy pulses with a peak negative pressure of 33.2 MPa and pulse repetition frequency of 40 Hz were applied along the clot. B-Mode and passive cavitation images were acquired during histotripsy insonation to monitor bubble activity. ResultsClots subjected to histotripsy with and without rt-PA exhibited greater thrombolytic efficacy than controls (7.0% flow recovery or lower), and histotripsy with rt-PA was more efficacious than histotripsy with saline (86.1 ± 10.2% compared with 61.7 ± 19.8% flow recovery). The addition of DEFINITY to histotripsy with or without rt-PA did not enhance either thrombolytic efficacy or cavitation dose. Cavitation dose generally did not correlate with thrombolytic efficacy. ConclusionEnhancement of thrombolytic efficacy was achieved using histotripsy, with and without catheter-directed rt-PA, in the presence of physiologic flow. This suggests these treatments may be effective as therapy for DVT.

Correlation of Escherichia coli inactivation with histotripsy bubble cloud size

Bacterial loads can be effectively reduced with cavitation-mediated focused ultrasound, or histotripsy. Our previous in vitro work with Escherichia coli (E. coli) established strong trends of bactericide with increasing peak negative pressure amplitude, pulse length, PRF, and treatment time. The current study correlates bactericide as a function of the histotripsy bubble cloud size produced for these treatment parameters at several frequencies. Histotripsy was applied to E. coli suspensions in 10-ml sample vials at 810 kHz, 1.2 MHz, or 3.25 MHz for 40 min. Separately, cavitation was recorded using a Photron Fastrax high-speed camera for acoustic parameters equivalent to those used in the E. coli studies. The images were used to quantify the maximum size of each bubble cloud, with the assumption that the cloud was axially symmetric around the propagation direction. A strong linear relationship exists between log kill versus cloud size (R2 = 0.96). Remarkably, across all variables studied, the log-reduction in viable bacteria exhibited a direct proportional relationship with the dimensions of the bubble cloud. This strong correlation between bacterial reduction and cavitation bubble cloud size could have significance for clinical applications where bacteria cannot easily be sampled. [Work supported by NIH R01AR080120 and R01EB023910.]

Cavitation cloud formation and surface damage of a model stone in a high-intensity focused ultrasound field

This work investigates the fundamental role of cavitation bubble clouds in stone comminution by focused ultrasound. The fragmentation of stones by ultrasound has applications in medical lithotripsy for the comminution of kidney stones or gall stones, where their fragmentation is believed to result from the high acoustic wave energy as well as the formation of cavitation. Cavitation is known to contribute to erosion and to cause damage away from the target, yet the exact contribution and mechanisms of cavitation remain currently unclear. Based on in situ experimental observations, post-exposure microtomography and acoustic simulations, the present work sheds light on the fundamental role of cavitation bubbles in the stone surface fragmentation by correlating the detected damage to the observed bubble activity. Our results show that not all clouds erode the stone, but only those located in preferential nucleation sites whose locations are herein examined. Furthermore, quantitative characterizations of the bubble clouds and their trajectories within the ultrasonic field are discussed. These include experiments with and without the presence of a model stone in the acoustic path length. Finally, the optimal stone-to-source distance maximizing the cavitation-induced surface damage area has been determined. Assuming the pressure magnitude within the focal region to exceed the cavitation pressure threshold, this location does not correspond to the acoustic focus, where the pressure is maximal, but rather to the region where the acoustic beam and thereby the acoustic cavitation activity near the stone surface is the widest.

Bubble cloud characteristics and ablation efficiency in dual-frequency intrinsic threshold histotripsy

Histotripsy is a non-thermal focused ultrasound ablation method that destroys tissue through the generation and activity of acoustic cavitation bubble clouds. Intrinsic threshold histotripsy uses single-cycle pulses to generate bubble clouds when the dominant negative pressure phase exceeds an intrinsic threshold of ∼25–30 MPa. The ablation efficiency is dependent upon the size and density of bubbles within the bubble cloud. This work investigates the effects of dual-frequency pulsing schemes on the bubble cloud behavior and ablation efficiency in intrinsic threshold histotripsy. A modular 500 kHz:3 MHz histotripsy transducer treated agarose phantoms using dual-frequency histotripsy pulses with a 1:1 pressure ratio from 500 kHz and 3 MHz frequency elements and varying arrival times for the 3 MHz pulse relative to the arrival of the 500 kHz pulse (−100 ns, 0 ns, and +100 ns). High-speed optical imaging captured cavitation effects to characterize bubble cloud and individual bubble dynamics. The effects of dual-frequency pulsing on lesion formation and ablation efficiency were also investigated in red blood cell (RBC) phantoms. Results showed that the single bubble and bubble cloud size for dual-frequency cases were intermediate to published results for the component single-frequencies of 500 kHz and 3 MHz. Additionally, bubble cloud size and dynamics were shown to be altered by the arrival time of the 3 MHz pulse with respect to the 500 kHz pulse, with more uniform cloud expansion and collapse observed for early (−100 ns) arrival. Finally, RBC phantom experiments showed that dual-frequency exposures were capable of generating precise lesions with smaller areas and higher ablation efficiencies than previously published results for 500 kHz or 3 MHz. Overall, results demonstrate dual-frequency histotripsy’s ability to modulate bubble cloud size and dynamics can be leveraged to produce precise lesions at higher ablation efficiencies than previously observed for single-frequency pulsing.

Histotripsy ablation for the treatment of feline injection site sarcomas: a first-in-cat in vivo feasibility study

Purpose Feline soft tissue sarcoma (STS) and injection site sarcoma (fISS) are rapidly growing tumors with low metastatic potential, but locally aggressive behavior. Histotripsy is a non-invasive focused ultrasound therapy using controlled acoustic cavitation to mechanically disintegrate tissue. In this study, we investigated the in vivo safety and feasibility of histotripsy to treat fISS using a custom 1 MHz transducer. Materials and Methods Three cats with naturally-occurring STS were treated with histotripsy before surgical removal of the tumor 3 to 6 days later. Gross and histological analyses were used to characterize the ablation efficacy of the treatment, and routine immunohistochemistry and batched cytokine analysis were used to investigate the acute immunological effects of histotripsy. Results Results showed that histotripsy ablation was achievable and well-tolerated in all three cats. Precise cavitation bubble clouds were generated in all patients, and hematoxylin & eosin stained tissues revealed ablative damage in targeted regions. Immunohistochemical results identified an increase in IBA-1 positive cells in treated tissues, and no significant changes in cytokine concentrations were identified post-treatment. Conclusions Overall, the results of this study demonstrate the safety and feasibility of histotripsy to target and ablate superficial feline STS and fISS tumors and guide the clinical development of histotripsy devices for this application.

Histotripsy Bubble Dynamics in Elastic, Anisotropic Tissue-Mimicking Phantoms.

Elastic, anisotropic tissue such as tendon has proven resistant to mechanical fractionation by histotripsy, a subset of focused ultrasound that uses the creation, oscillation and collapse of cavitation bubbles to fractionate tissue. Our objective was to fabricate an optically transparent hydrogel that mimics tendon for evaluation of histotripsy bubble dynamics. Ex vivo bovine deep digital flexor tendons were obtained (n=4), and varying formulations of polyacrylamide (PA), collagen and fibrin hydrogels (n=3 each) were fabricated. Axial sound speeds were measured at 1 MHz for calculation of anisotropy. All samples were treated with a 1.5-MHz focused ultrasound transducer with 10-ms pulses repeated at 1 Hz (p+=127 MPa, p-=35 MPa); treatments were monitored with passive cavitation imaging and high-speed photography. Dehydrated fibrin gels were found to be the most similar to tendon in cavitation emission energy (fibrin=0.69 ± 0.24, tendon=0.64 ± 0.19 [× 1010 V2]) and anisotropy (fibrin=3.16 ± 1.12, tendon=19.4). Bubble cloud area in dehydrated fibrin (0.79 ± 0.14 mm2) was significantly smaller than most other tested hydrogels. Finally, anisotropy was found to have moderately strong linear relationships with cavitation energy and bubble cloud size (r=-0.65 and -0.80, respectively). Dehydrated fibrin shows potential as a repeatable, transparent, tissue-mimicking hydrogel for evaluation of histotripsy bubble dynamics in elastic, anisotropic tissues.

Experimental investigation of two-phase flow evolution in a high-speed submerged water jet with air ventilation

In order to improve the submerged cutting performance of water abrasive suspension jet an experimental investigation on the two-phase flow structure of submerged water jet with semi-coaxial air ventilation is conducted by the method of high-speed camera observation under the condition of flow dynamic similarity, and its unsteady behavior under different conditions is evaluated by analyzing of sequence images. Experiment results show that the flow pattern of air-ventilated jet depends upon the ventilation flow rate. It may be classified into bubbly flow surrounded jet, continuous air-layer covered jet and cavity-bubble flow surrounded jet. In the case of non-ventilation, high-speed water jet into water is accompanied by intensive cavitation and the flow field appears to be a narrow jet surrounded by cavitation bubble clouds. By ventilating to the cavitating jet, air-bubbles become bigger gradually and a cavity area is formed at the exit of nozzle head. When the ventilation flow ratio reaches to the level of 5 a large cavity covering the jet is formed and a continuous air-layer covered jet is then generated. Image analysis demonstrates that the continuous air-layer covering the liquid jet pulsates and sheds downstream periodically. The Strouhal number defined by the dominant frequency of jet pulsation decreases quickly with the increase of ventilation flow rate in the range of Cq ≤ 5. At a suitable ventilation flow ratio (Cq ≈ 6 ~ 8) a relative long stable continuous air-layer covering the jet is generated at high time ratio, which will dramatically enhance the processing ability of submerged water jets. Cutting tests demonstrates that the cutting ability of air ventilated jet depends on the ventilation flow ratio. The relation of processing ability with the flow structure of air ventilated jet is clarified.

Synchrotron X-ray imaging of the onset of ultrasonic horn cavitation.

High-power ultrasonic horns operating at low frequency are known to generate a cone-shaped cavitation bubble cloud beneath them. The exact physical processes resulting in the conical structure are still unclear mainly due to challenges associated with their visualization. Herein, we address the onset of the cavitation cloud by exploiting high-speed X-ray phase contrast imaging. It reveals that the cone formation is not immediate but results from a three-step phenomenology: (i) inception and oscillation of single bubbles, (ii) individual cloud formation under splitting or lens effects, and (iii) cloud merging leading to the formation of a bubble layer and, eventually, to the cone structure due to the radial pressure gradient on the horn tip.

DNA release from plant tissue using focused ultrasound extraction (FUSE).

Sample preparation in genomics is a critical step that is often overlooked in molecular workflows and impacts the success of downstream genetic applications. This study explores the use of a recently developed focused ultrasound extraction (FUSE) technique to enable the rapid release of DNA from plant tissues for genetic analysis. FUSE generates a dense acoustic cavitation bubble cloud that pulverizes targeted tissue into acellular debris. This technique was applied to leaf samples of American chestnut (Castanea dentata), tulip poplar (Liriodendron tulipifera), red maple (Acer rubrum), and chestnut oak (Quercus montana). We observed that FUSE can extract high quantities of DNA in 9-15 min, compared to the 30 min required for control DNA extraction methods. FUSE extracted DNA quantities of 24.33 ± 6.51 ng/mg and 35.32 ± 9.21 ng/mg from American chestnut and red maple, respectively, while control methods yielded 6.22 ± 0.87 ng/mg and 11.51 ± 1.95 ng/mg, respectively. The quality of the DNA released by FUSE allowed for successful amplification and next-generation sequencing. These results indicate that FUSE can improve DNA extraction efficiency for leaf tissues. Continued development of this technology aims to adapt to field-deployable systems to increase the cataloging of genetic biodiversity, particularly in low-resource biodiversity hotspots.

INFLUENCE OF TISSUE STIFFNESS ON CAVITATION CLOUD FORMATION BY HIGH INTENSITY FOCUSED ULTRASOUND IN AGAROSE GELS

The effects of tissue stiffness on the growth and collapse of a laser-induced bubble and the cavitation cloud formation by high intensity focused ultrasound (HIFU) in tissue-mimicking phantoms were investigated. First, the growth and collapse of a laser-induced bubble were observed with a high-speed video camera, which showed that the maximum bubble radius decreases with increase in the stiffness of agarose gels. The period of bubble collapse becomes shorter as the stiffness increases in agarose gels. However, the period in 0.3% agarose is longer than that in 7% gelatin, although both Young's moduli are similar to each other. In 0.3% and 0.5% agarose gels, the laser-induced bubble enlarged with the generation of wrinkles on its interface. Experiments were also conducted for the cavitation cloud formation due to the backscattering of HIFU from the residual microbubbles which remain after the laser-induced bubble collapses. In 0.3%, 0.7%, and 1.5% agarose phantoms, the cavitation clouds developed along with the propagation axis of HIFU, while a dense cavitation bubble cloud was not observed in 3.0% agarose. As the gel stiffness increases, the size of the cavitation cloud decreases due to the high stiffness of the surrounding medium. Also, the collapse time of a cavitation cloud becomes shorter with increase in the stiffness of agarose gels. Although the Young's modulus of 6% gelatin is smaller than 0.3% agarose, the collapse time of the bubble cloud is shorter than that in 0.3% agarose.

Thermodynamics of the inception and interactions of multiple laser-produced cavitation bubbles using the lattice Boltzmann method

Understanding the thermodynamics associated with the inception and interactions of multiple cavitation bubbles is vital in developing deeper insights into the flow field and temperature properties of cavitation bubble clouds. This paper describes a double-distribution-function thermal lattice Boltzmann method for investigating the thermodynamics of multiple-bubble interactions, and proposes a new two-dimensional method for cavitation bubble inception without the initialization of gas nuclei. This approach is validated by simulating the evolution of a laser-produced bubble. The interaction between two vapor bubbles is simulated, and the hydrodynamics and thermodynamics of cavitation bubbles are systematically investigated. Both weak and strong interactions among bubbles of equal size are accurately reproduced. The coalescence mode is further categorized according to differences in the collapse behaviors: the remaining parts collapse away from each other for coalescence in the collapse stage, but collapse toward each other for coalescence in the growth stage. Interactions among bubbles of different sizes are also explored and the different morphologies for various interaction modes are reported. In the case of small distances between bubbles under the weak interaction regime, the smaller bubble is pushed away from the symmetry axis, leading to an initial decrease in collapse intensity and then an increase as the distance between the two bubbles increases. Finally, the thermodynamics of a bubble cluster are investigated. The hydrodynamic and thermodynamic processes in different regions of the cavitation bubble cluster are determined, and the toroidal shape in the final stage is reproduced. The results demonstrate that the proposed model accurately simulates the complex interactions among multiple cavitation bubbles.

ゼラチン内の気泡界面での集束超音波の後方散乱により形成されるキャビテーションクラウドに対する気泡径の影響

Cavitation cloud formation by the backscattering of focused ultrasound from a bubble interface in gelatin has been studied experimentally. A laser-induced bubble which is generated near the geometrical focus of the focused ultrasound is used as a reflector of the incident wave to yield strong negative pressure which leads to the cavitation inception and following cavitation bubble cloud formation. The maximum size of the laser-induced bubble is about 2 mm in radius. After the laser-induced bubble collapses, a small bubble whose radius is about 0.3 mm remains around the laser focus. In the present experiment, this residual bubble is also used as a reflector bubble, and the effect of reflector bubble size on cavitation cloud formation has been evaluated. Cavitation inception and cavitation cloud formation near the reflector bubble has been observed with a high-speed camera. It is shown that the cavitation cloud grows along with the propagation axis of the incident wave. The larger the reflector bubble size becomes, the earlier the first cavitation inception near the reflector occurs. On the other hand, regardless of the reflector bubble size, the distance between the top interface of the cavitation cloud and the geometrical focus of the focused ultrasound at the maximum expansion of the cloud is almost constant. The results also show that when a residual bubble is used as a reflector, the growth rate of a cavitation cloud in the direction of the propagation axis becomes higher than that when a laser-induced bubble is used. This may be due to the difference of the interface shape of the tip of developing cavitation cloud.

A sharp-interface model for grid-resolved cavitating flows

A sharp-interface model for cavitating flows is presented in this work. The proposed model can deal with the dynamic evolution of cavitation, and considers both phase change and pre-existing gas expansion mechanisms. The interfaces between the liquid and gas phases are fully sharp, the effects of compressibility (in both phases) and surface tension are considered, and the liquid may withstand certain amounts of tension before breaking down. The formulation is in principle independent of grid discretization. The method is simple to implement, and avoids raising the computational cost as it does not require the solution of additional transport equations. The behaviour of the proposed model is thoroughly assessed through a series of numerical tests. Finally, the developed method is used to predict the nucleation and collapse of a three-dimensional cavitation bubble cloud.

The hydroxyl radical yield prediction of cavitation bubble clouds during the hydrodynamic cavitation process for chitosan degradation

In order to measure the influence of chemical effects in the process of hydrodynamic cavitation (HC) degradation of chitosan, a prediction model for the hydroxyl radical (˙OH) yields of cavitation bubble clouds (CBCs) was developed based on a single-hole orifice plate cavitator.

Experiments and numerical analysis of rebounding behaviors of a laser-induced cloud cavitation

It is well known that a high-speed submerged water jet generates a cloud of cavitation bubbles experiencing a collective unsteady behavior that repeats growth and collapse. In particular, it is considered that a high-pressure shock wave may be emitted associated with the collapse of the cloud. However, its mechanism has not been enough clarified and hence it is required to be explored both from the experimental and numerical analyses. In this study, we fist make an observation of such a unsteady behavior of the cloud cavitation that is generated by a pulsed Ho:YAG laser-induced liquid jet using a high-speed video camera. Then, a two-dimensional numerical analysis based on the SPH method is examined to simulate the unsteady behavior of the cloud, in which the mixture model of liquids and gas bubbles is employed. Finally, the validity of our numerical analysis is illustrated by showing that the unsteady behavior of the cloud from its inception, growth, shrink, collapse to rebound is realized in qualitative agreement with the experimental data.

The influence of medium elasticity on the cavitation cloud formation by the backscattering of focused ultrasound from a bubble interface

High Intensity Focused Ultrasound (HIFU) is utilized for a minimal invasive method to ablate tumors by the temperature rise at the focal point of the focused ultrasound. In HIFU therapy, the treatment time tends to be long due to the narrow region of the ablation. Hence, the ways to enhance the efficiency of HIFU therapy have been studied, and the usage of a dense cavitation bubble cloud is expected to enhance energy dissipation from acoustic energy to heat. However, the cavitation cloud may cause the damage of surrounding tissue due to shockwaves when it collapses. Therefore, it is necessary to control cavitation clouds for effective usage. Ogasawara et al. (2018) investigated the cavitation cloud formation in water by using a laser-induced bubble as a reflector of HIFU. In the present study, we investigate the influence of medium elasticity on the cavitation cloud formation by the backscattering of HIFU from microbubbles created by a focused laser beam in the elastic medium. As a tissue mimicking phantom, agarose gel is chosen because of its high optical clarity for photography and its wide usage in tissue engineering. Medium elasticity varies from approximately 2.7 kPa to 200 kPa. By using residual microbubbles in the gel as reflectors of HIFU, the cavitation cloud generation point can be controlled. It is shown that as gel stiffness increases, the maximum value of the top interface position of cavitation clouds decreases and the collapse time of cavitation clouds becomes shorter.