Cats In Urban Areas Research Articles

A Situational Analysis of Attitudes toward Stray Cats and Preferences and Priorities for Their Management.

Current cat management approaches are outdated and ineffective, failing to reduce stray cat numbers or related complaints and negatively impacting the job satisfaction and mental health of veterinary, shelter, and municipal staff. We undertook a situational analysis prior to implementing a Community Cat Program based on free sterilization of owned, semi-owned, and unowned cats in the city of Ipswich, Queensland, Australia. The study involved 343 residents in three suburbs in Ipswich, Queensland, Australia with high per capita intake of cats into the receiving shelter and municipal pound. We investigated the prevalence and impacts of free-roaming cats in urban areas, focusing on sightings, associated issues, and community preferences for cat management. Stray cats were observed by many respondents (71%), primarily at private residences (52%) and in alleyways or streets (22%), which caused serious or moderately serious problems for 38% of those who saw stray or wandering cats. Key concerns included the killing of native birds (38%) and animals (35%), noise (33%), and soiling (32%). Actions taken by respondents who saw stray or wandering cats included chasing them away or using deterrents (25%), capturing the cat for removal or calling council (18%) and preventing home entry (14%). Respondents' priorities for the local government management of cats included preventing kittens from being born (94% of respondents) and stopping cats from preying on native animals (91%); reducing disease spread to pets (89%), wildlife (89%), and humans (87%); decreasing stray cat numbers (75%); and preventing cat fights (70%). Respondents favored sterilization (65%) over euthanasia (35%), aligning with the results of previous research. Cat ownership and feeding unowned cats were predictors of management preferences. Only 29% of respondents were satisfied with the current local council management of the problem. Information on the benefits of management by sterilization could further enhance community support.

Situational Analysis of Cat Ownership and Cat Caring Behaviors in a Community with High Shelter Admissions of Cats.

Managing stray cats in urban areas is an ongoing challenge, and in Australia, many are euthanized. Most stray cats are from disadvantaged areas and are under 1 year of age. The Australian Pet Welfare Foundation intended to assess the impact of a free cat sterilization program in an area with high shelter intake of cats in the city of Ipswich, Queensland. The aims of this pre-intervention study were to undertake a situational analysis of cat ownership, semi-ownership and cat caring behaviours, and compare those in the same demographic with dog ownership and caring behaviours relating to sterilization rates, to provide a basis against which to assess the program's effectiveness. In a sample of 343 participants from that area, 35% owned cats and 3% fed stray cats. Cats were predominantly obtained from family or friends (31%) and shelters (20%). More respondents owned dogs (53%), which were most often sourced from breeders (36%) and family acquaintances (24%). More owned cats than owned dogs were sterilized (91% versus 78%). However, only 74% of cats aged 4 to <12 months were sterilized. Cat containment practices varied, with 51% of owners containing their cat(s) at all times, and a further 18% doing so at night. These results suggest the need for community-based programs that focus on sterilizing owned and semi-owned cats, and assisting semi-owners in becoming full owners to reduce stray cat populations and associated negative impacts. This includes assistance with cat containment where vulnerable native species are present. Public understanding of the causes and effective solutions for free-roaming cats, alongside legislative changes, are required to facilitate these efforts. Assistive programs aligned with One Welfare principles are expected to benefit the wellbeing of animals, humans and their environments.

Public Attitudes towards and Management Strategies for Community Cats in Urban China.

Managing community cats in urban China is a contentious and emerging issue, with debates centering on the most effective and humane approaches. This study aimed to investigate public attitudes towards community cats and various management strategies. A survey was conducted involving 5382 urban residents in China. Their attitudes towards the positive and negative roles of community cats in urban areas and their support for different management methods were examined, including trap-and-kill, taking no action, centralized management, and trap-neuter-return (TNR) and its variations. Results indicated that 63% of participants were willing to coexist with community cats, 71% opposed trap-and-kill, and 61% agreed or strongly agreed with the TNR method and its variations. Older residents or those with higher incomes were more likely to support coexistence with community cats. In contrast, younger or lower-income residents were more likely to support non-coexistence. Residents in first- or second-tier cities (e.g., Beijing, Hangzhou, and Jinan Cities in China) were more inclined to support trap-and-kill and less likely to support coexistence than their counterparts in fourth-tier cities (e.g., county-level cities in China). Moreover, those with lower education or incomes were more supportive of trap-and-kill and taking no action as the methods to manage community cats than those with relatively higher education or incomes. Those with higher incomes held more positive attitudes towards community cats and were more supportive of TNR and its variations than their counterparts with lower incomes. Males were more inclined to support trap-and-kill and taking no action and less inclined to support centralized management and TNR than females. The implications of the findings on TNR with adoption programs in urban China are discussed. These novel findings underscore the need for targeted educational campaigns to promote humane and effective management strategies, addressing public concerns and community cats' welfare. The study's insights are critical for informing policy and improving community cat management in urban China.

How Lazy Are Pet Cats Really? Using Machine Learning and Accelerometry to Get a Glimpse into the Behaviour of Privately Owned Cats in Different Households.

Surprisingly little is known about how the home environment influences the behaviour of pet cats. This study aimed to determine how factors in the home environment (e.g., with or without outdoor access, urban vs. rural, presence of a child) and the season influences the daily behaviour of cats. Using accelerometer data and a validated machine learning model, behaviours including being active, eating, grooming, littering, lying, scratching, sitting, and standing were quantified for 28 pet cats. Generalized estimating equation models were used to determine the effects of different environmental conditions. Increasing cat age was negatively correlated with time spent active (p < 0.05). Cats with outdoor access (n = 18) were less active in winter than in summer (p < 0.05), but no differences were observed between seasons for indoor-only (n = 10) cats. Cats living in rural areas (n = 7) spent more time eating than cats in urban areas (n = 21; p < 0.05). Cats living in single-cat households (n = 12) spent more time lying but less time sitting than cats living in multi-cat households (n = 16; p < 0.05). Cats in households with at least one child (n = 20) spent more time standing in winter (p < 0.05), and more time lying but less time sitting in summer compared to cats in households with no children (n = 8; p < 0.05). This study clearly shows that the home environment has a major impact on cat behaviour.

A Survey of Public Opinion on Community Cats’ General Health and Relationship Quality with Residents in Urban China

The management and coexistence of community cats in urban areas is a growing concern amid global urbanization. Through a survey-based investigation, we examine the residents’ perceptions of the general health of community cats and human-cat relationships in urban China. The data from 5382 participants revealed that approximately 70% of participants perceived community cats as being in good health, and 60% reported harmonious or non-conflict coexistence between residents and these cats. Around 45% of the participants rescued or helped community cats, 38% expressed their intention to adopt, and 18% complained about the issues of community cats to management staff. Linear, logistic, and multilevel-logistic regressions were employed to examine the associations between the types of cities and communities or the participants’ socio-demographics and the perceived well-being of community cats or human-cat relationships. The results show that the cats in fourth-tier cities (e.g., county-level cities) had poorer living conditions than in first-tier cities (e.g., Beijing), while the cats in urban village communities (e.g., villages in the city) were less likely to exhibit good health than in ordinary commercial housing communities. The results also show that socio-demographic variables, such as educational attainment, marital status, and income level, predicted participants’ relationships with community cats. This study is the first of its kind. It provides valuable insights for stakeholders to develop effective policies and interventions on cat management, emphasizing the need for tailored strategies in diverse urban settings and populations.

Estimates of Domestic Cats in Urban Areas Using Interdisciplinary Science: The Washington D.C. Cat Count

Abstract Accurate information about the number of cats living outdoors and how they respond to different kinds of management are necessary to quell debates about outdoor cat policy. The DC Cat Count will develop the tools and methodologies needed to realize this possibility and make them available for broader use. This three-year initiative represents a major collaboration between animal welfare organizations and wildlife scientists. Its unique and innovative approach is to use the best scientific methods to quantify all subpopulations of cats in the District of Columbia (outdoor, owned, and shelter cats), concurrently test and optimize simpler methods that can be used to measure cat populations by diverse users at scale, and identify the types of interventions that are likely to accomplish desired outcomes most efficiently. Ultimately, we believe that this approach is more likely to improve outcomes for both cats and wildlife than a continuation of the status quo.

Antimicrobial Drug-Resistant Salmonella in Urban Cats: Is There an Actual Risk to Public Health?

The present study was undertaken to investigate the presence of Salmonella spp. in the faeces of client-owned cats in urban areas and to evaluate the risk that is posed to public health. Fresh faecal samples were collected directly from the rectums from 53 diarrhoeic and 32 non-diarrhoeic cats. The samples were individually screened for the presence of Salmonella spp. using standard methods and, in the case of positive findings, the resulting typical colonies were then biochemically confirmed using the VITEK®2 automated system. Subsequently, all of the Salmonella spp. isolates were molecularly tested for the presence of the invA gene. All of the isolates were serotyped using the slide agglutination technique according to the White–Kauffmann–Le Minor scheme. The phenotypic antimicrobial susceptibility profile of the isolated strains was obtained from the VITEK®2 system using specific cards from the Gram-negative bacteria. A total of 16 of the samples (18.82%) tested positive for Salmonella spp. according to conventional and molecular testing methods. Serotyping of the Salmonella isolates showed the presence of three serotypes, namely S. enteritidis (n = 9; 56.3%), S. typhimurium (n = 4; 25%), and S. kentucky (n = 3; 18.8%). All of the tested strains showed strong resistance towards cefazolin, cefepime, ceftazidime, and ceftriaxone. Additionally, resistance (listed in descending order of strength) was observed to trimethoprim/sulfamethoxazole (11/16; 68.8%), ampicillin (10/16; 62.5%), ampicillin/sulbactam (9/16; 56.3%), gentamicin (9/16; 56.3%), nitrofurantoin (8/16; 50.0%), and amikacin (5/16; 31.3%). No resistance was expressed against ciprofloxacin, ertapenem, imipenem, levofloxacin, piperacillin/tazobactam, and tobramycin. The results of this study highlight a substantial public health issue and medical concern, especially in vulnerable people, such as children, the elderly, and immunocompromised individuals.

An Investigation on the Cases of Bitten by Animals in Yazd City

Common diseases between humans and animals are an important public health priority. The aim of this study was to evaluate the epidemiological characteristics of animal bite referring to rabies prevention centers in Yazd University of Medical Sciences in 2018. This is a cross-sectional descriptive study. All cases of animal bite were examined in the rabies prevention center of Yazd city during 2018. Demographic and epidemiological data were analyzed using SPSS 19 software. The results of the study show that 1,750 cases of bite occurred in adolescents and males. In this study, the most common bite was in the age group of 15 to 19 (33.3%) in terms of age distribution. The most common animal was bitten by cats in urban areas. According to the bite season, the highest bite in this study was summer and the lowest in spring. Given the high incidence of bite in the province, it is recommended to plan for educational and care activities to reduce the incidence of bite

Weak Effects of Owned Outdoor Cat Density on Urban Bird Richness and Abundance

Domestic cats (Felis catus) are ubiquitous predators of birds in urban areas. In addition to the lethal effect of predation, there can also be sublethal, negative effects of domestic cats on individual birds. These effects have led to the inference that reducing outdoor cat densities would benefit urban bird communities. Here we estimate the likely result of policies/programs designed to reduce densities of owned outdoor cats in urban areas, estimating relationships between bird richness/abundance and cat densities across 58 landscapes in Ottawa, Ontario, Canada. We estimate that we would most likely observe one additional bird species, and 0.003 additional individuals per species, if policies/programs reduced owned outdoor cat densities to zero in an average landscape in Ottawa (with 130.2 cats/km2). However, these effects of cat density on birds were uncertain, with 95% confidence intervals crossing zero. Our findings—in combination with those of previous studies—suggest a need for research to resolve the apparent disconnect between the strong, negative effects of cats on individual urban birds and the weak, uncertain effects of cats on bird populations. Although measures that reduce owned outdoor cat densities are justified based on the precautionary principle, evidence to date does not support prioritizing these measures over those addressing threats that have consistently strong effects on bird populations.

Toxoplasma gondii in domiciled dogs and cats in urban areas of Brazil: risk factors and spatial distribution.

Toxoplasma gondii is a highly prevalent zoonotic parasite in Brazil capable of infecting mammals and birds. The increase in the urban populations of pets and the narrowing of the human–animal relationship can facilitate the transmission of important public health zoonoses, such as toxoplasmosis. This study aimed to evaluate the frequency and spatial distribution of T. gondii infection and its risk factors in domiciled dogs and cats attended at the Jorge Vaitsman Institute, Rio de Janeiro. Serum samples from 400 dogs and 272 cats were evaluated by an indirect fluorescent antibody test (IFAT) for IgG anti-T. gondii antibodies. Epidemiological questionnaires were used to interview the animals’ owners to identify risk factors for infection. Of the total, 34% (136/400) of dogs and 8.1% (22/272) of cats had anti-T. gondii antibodies. Breed (OR: 2.10–95%, CI 1.27–3.46) was a risk factor for dogs, while sex (OR: 3.40–95%, CI 1.10–10.52) and homemade food consumption (OR: 8.49–95%, CI 2.48–29.05) were risk factors for cats. Offal consumption was considered a risk factor for both species evaluated (OR: 2.74–95%, CI 1.38–5.43 for dogs; OR: 7.66–95%, CI 1.24–47.29 for cats). The spatial analysis showed that T. gondii seropositive animals were widely distributed in the metropolitan region of Rio de Janeiro state, with a concentration observed mainly in the west and north zones of Rio de Janeiro city. The results emphasize the importance of adopting prophylactic measures to control T. gondii transmission in domiciled dogs and cats in Rio de Janeiro, contributing positively to public health.

Clinical-parasitological Screening for Respiratory Capillariosis in Cats in Urban Environments.

SummaryRespiratory capillariosis is a widely distributed zoonotic parasitic disease caused by the nematode Capillaria aerophila (Trichocephalida, Trichuridae) that commonly infects wild carnivores but also cats and dogs. This retrospective study aims to describe cases of respiratory capillariosis in cats from the city of Belgrade, Serbia. Between 2015 and 2019, a total of 155 pet cats with or without respiratory symptoms were submitted to physical examination and parasitological examination of the feces. All cats lived indoor but had free access to outdoor. In suburban settlements, wild carnivores commonly share their living environments with owned cats and dogs. It can be assumed that more intense urbanization spreading into the natural habitats of will carnivores creates the opportunity for closer and more frequent contacts between the population of cats and feral carnivores which might increase the risk of feline contamination. The findings confirm the existence of capillaries in cats in urban areas of the city of Belgrade, contribute to a better understanding of the epidemiology of this nematode and warn that, because of close contacts between cats of pets and humans, capillaries can cause human infection.

Scenario of the prevalence of parasites in soil/sand samples in Brazil

Introduction: Zoonotic diseases are transmitted from animals to humans, compromising their health. The high concentration of dogs and cats in urban areas plays an important epidemiological role on soil contamination and thus in the dissemination of zoonotic diseases. The ingestion of eggs, cysts or oocysts of parasites and the penetration of helminths larvae in the skin are frequently related to environmental contamination. Objective: The aim of this study was to assess the prevalence of parasites in soil and/or sand samples in Brazil by a systematic review. Methods: A total of 43 papers were selected from SciELO Brasil, LILACS-Bireme PubMed/NCBI and Scopus, published between 2009 and 2019. The search was performed using the tems parasites, soil, sand and Brazil. Results: A higher number of publications occurred from 2012 to 2017. Studies were mainly concentrated in Southeast and South regions. Hence, further studies are needed in regions where there is limited basic sanitation. Ancilostomidae (larvae or eggs) were the most frequent parasites found in 41.8% of studies, followed by Toxocara spp. (32.6%) and Ascaris spp. (14%). Conclusion: The knowledge on the presence of parasites in the environment and its potential of contamination to the population is essential. Thus, the promotion of educational campaigns related to the implementation of zoonotic control is required in order to avoid the dissemination of parasites in the environment. Keywords: Parasites. Sand. Soil. Zoonoses. Brazil.

Seroprevalence and associated risk factors of Toxoplasma gondii infection in stray cats in Algiers urban area, Algeria

Toxoplasmosis is a zoonotic parasitic disease caused by the protozoan Toxoplasma gondii. Human infections are common and generally asymptomatic, but they can become very dangerous in immunosuppressed and HIV-positive patients. The infection can also be serious if it is transmitted to the fetus during pregnancy. Infection in untreated mothers can lead to abortion, birth defects and blindness of the fetus. The aim of this study is to determinethe seroprevalence of Toxoplasma IgT antibodies in cats in the urban area of Algiers. From December 2017 to August 2018, blood samples from 184 stray cats were collected and analyzed for IgG antibodies against T. gondii using an ELISA method. Overall, the prevalence of T. gondii infection in stray cats was 58.15% (107/184). There was no statistically significant difference between male and female cats. The rate of seropositivity of T. gondii increased with age (p &lt;0.05). There was no statistically significant difference between the different regions from which the samples were taken. The results of the present study showed the high seroprevalence of toxoplasmosis in Algiers rather than in other countries. Due to the high seroprevalence of Toxoplasma IgG antibodies in cats, it is recommended to include in the prenatal evaluation, together with the adoption of a screening test and the determination of the IgG antibody titer in the high-risk populations (young girls, pregnant women) public information programs on the disease and measures that can contribute to prevention

The Prevalence of Endoparasites of Free Ranging Cats (Felis catus) from Urban Habitats in Southern Poland.

Simple SummaryFree ranging domestic cats are common in urban and suburban habitats and may cause a threat of disease transmission to other pets, wildlife and humans. We investigated the occurrence of endoparasites in cats in Kraków city, Southern Poland, based on examination of road-killed individuals. More than half of the cats were infected with at least one of seven identified parasites. Cats from suburban areas were more likely to be infected than cats from the city core. Our study supports the results from other studies, showing that cats may serve as a significant source of zoonotic transmission.Growing urbanization leads to an increased risk of parasite spread in densely inhabited areas. Free-ranging cats can be locally numerous and come into frequent contact with both wildlife and humans. Cats are thus expected to contribute to parasitic disease transmission. In our study, we investigated the prevalence of endoparasites in free ranging cats in urban areas of Kraków city, based on necropsy of road-killed cats in relation to sex and diet of cat, season and habitat type. We found that 62% of 81 cats were infected with endoparasites with Toxocara cati being the most prevalent. In total, we identified seven parasite species. The number of parasite species was higher in suburban habitats and aside from Eucoleus aerophilus the prevalence of all parasites was higher in cats from suburban areas than in the individuals living in the city urban core. The prey of examined cats included mostly rodents, followed by soricomorphs and birds, which can all serve as paratenic hosts. Based on our results, we suggest that cats in urban areas should be considered as a serious potential zoonotic threat. Implementation of proper veterinary control and wider education on the topic is recommended.

Integrating Trap-Neuter-Return Campaigns Into a Social Framework: Developing Long-Term Positive Behavior Change Toward Unowned Cats in Urban Areas.

Cat management is often discussed in terms of population reduction, with trap-neuter-return (TNR) campaigns commonly organized to manage unowned urban cat populations. However, long-term effectiveness is only possible if positive neutering practices are continued by local residents. Here we discuss how implementing TNR within a wider framework of social engagement has the potential to tackle cat overpopulation and instill long-term positive behavior change toward them. We demonstrate how community engagement pre-TNR can help establish a baseline of the attitudes, knowledge and behavior concerning cats. Using a case study, we explore whether this information can be linked with positive intended behavior based on intentions to arrange for neutering of unowned cats. Structural equation modeling indicated that negative attitudes toward cats and reduced knowledge around neutering reduced the likelihood of positive intended behavior. This result was underpinned by the indirect effects of perceptions of unowned cats and reduced understanding of their needs. Utilizing these results alongside an understanding of the values and motivation of the community allows for tailored and targeted education and intervention. In turn, this addresses the underlying knowledge gaps and perceptions regarding cat welfare. This framework can help address the challenge of cat management because it: (1) takes an integrative approach to identifying the motivations of communities to take responsibility for unowned cats; (2) changes the structure of the social environment, encouraging positive neutering practices for unowned cats. In turn this improves the impact and longevity of TNR campaigns whilst promoting positive welfare change for unowned and owned cats; and (3) appreciates that opinions are likely to vary hugely between areas, therefore providing an adaptable community level approach.

Ticks and tick-borne pathogens of cats in the UK

Ticks parasitising cats are widely distributed throughout the UK, and awareness of the risk presented by ticks and the pathogens they may transmit is of importance. Higher tick numbers and year-round biting activity could pose a significantly increased threat to cats and their owners. The most frequently recorded tick on cats is Ixodes ricinus, but the hedgehog tick Ixodes hexagonus is also common particularly for cats in urban areas and is considerably more prevalent on cats than on dogs. Ticks transmit a wide range of pathogens to cats, although usually with relatively low prevalence in the UK. However, of note is that, in a recent study of the pathogens present in ticks feeding on cats, Babesia vulpes was detected in I. hexagonus and B. venatorum was detected in I. ricinus; both are agents of potentially fatal diseases and in the latter case the pathogen is zoonotic. Tick control is therefore important in cats, not only for the health of parasitised individuals, but also to prevent cats acting as transport hosts for ticks carrying a wide range of pathogens of both veterinary and zoonotic significance.

Multistate matrix population model to assess the contributions and impacts on population abundance of domestic cats in urban areas including owned cats, unowned cats, and cats in shelters.

Concerns over cat homelessness, over-taxed animal shelters, public health risks, and environmental impacts has raised attention on urban-cat populations. To truly understand cat population dynamics, the collective population of owned cats, unowned cats, and cats in the shelter system must be considered simultaneously because each subpopulation contributes differently to the overall population of cats in a community (e.g., differences in neuter rates, differences in impacts on wildlife) and cats move among categories through human interventions (e.g., adoption, abandonment). To assess this complex socio-ecological system, we developed a multistate matrix model of cats in urban areas that include owned cats, unowned cats (free-roaming and feral), and cats that move through the shelter system. Our model requires three inputs—location, number of human dwellings, and urban area—to provide testable predictions of cat abundance for any city in North America. Model-predicted population size of unowned cats in seven Canadian cities were not significantly different than published estimates (p = 0.23). Model-predicted proportions of sterile feral cats did not match observed sterile cat proportions for six USA cities (p = 0.001). Using a case study from Guelph, Ontario, Canada, we compared model-predicted to empirical estimates of cat abundance in each subpopulation and used perturbation analysis to calculate relative sensitivity of vital rates to cat abundance to demonstrate how management or mismanagement in one portion of the population could have repercussions across all portions of the network. Our study provides a general framework to consider cat population abundance in urban areas and, with refinement that includes city-specific parameter estimates and modeling, could provide a better understanding of population dynamics of cats in our communities.

Detection of Vaccinia Virus in Urban Domestic Cats, Brazil.

We investigated possible vaccinia virus (VACV) in urban house cats in Brazil. Serum samples from 6 cats were positive for VACV by PCR, indicating likely VACV circulation among house cats in urban areas of Brazil. This finding highlights the importance of epidemiologic surveillance to avoid outbreaks among urban human populations.

Investigation of urban ethnoveterinary in three veterinary clinics at east zone of São Paulo city, Brazil

Ethnopharmacological relevanceUrban Ethnoveterinary should be further explored because of its value as it resists conventional medical care. In addition, the comparison between the resources used by Ethnoveterinary and Ethnopharmacology should be investigated in depth, increasing the availability of new bioactive potential in human and veterinary medicines. This project aimed to determine whether plants are used in the health care of dogs and/or cats in urban area and to compare its uses with other ethnoveterinary and ethnopharmacological data. Materials and methodsThree veterinary clinics from east area of São Paulo city were selected, in order to record the offering of plants to pets by their owners. Individual interviews were conducted from May to November 2012 and consisted of application of semi-structured interviews and questionnaires. A literature search was performed to compare our findings with other ethnopharmacological and ethnoveterinarian data from the literature. ResultsA total of 273 people were interviewed and 46 (16.84%) of them utilize medicinal plants for their pets. Most plant species are exclusively home grown (57.9%). The plants most cited were Plectranthus barbatus Andrews, Matricaria chamomilla L. and Foeniculum vulgare Mill. The most frequent route of administration, part utilized and mode of preparation was oral (80.35%), leaves (89.47%) and infusion (61.90%), respectively. From 19 cited plants, 14 (73.7%) are mentioned in ethnoveterinary literature, whereas 11 (57.9%) are used for the same purposes. All plants reported in our study have at least one common use with ethnopharmacology. ConclusionThe survey provided evidence of ethnoveterinary use of medicinal plants for dogs and/or cats in urban area of São Paulo, complementarily with the official veterinary, and showed that the plants used in pets are also used in humans with the same purposes and routes of administration, as well as in other animals.

Investigation of endoparasite community of dogs and cats in urban areas in South-Eastern Romania: Potential risks for animal and public health

Investigation of endoparasite community of dogs and cats in urban areas in South-Eastern Romania: Potential risks for animal and public health