Categories Of Resistance Research Articles

Amino acid substitutions in succinate dehydrogenase complex conferring resistance to the SDHI fungicide pydiflumetofen in Cochlibolus heterostrophus causing southern corn leaf blight1

Southern corn leaf blight (SCLB) caused by Cochlibolus heterostrophus, is a widespread foliar disease that has a substantial impact on maize yield in the Huanghuaihai region of China. Pydiflumetofen (Pyd), a new succinate dehydrogenase inhibitor (SDHI), has been found as a promising fungicide for the efficient control of SCLB, however, resistance of C. heterostrophus to Pyd has not been studied well. Here, five Pyd-resistant mutants were generated through fungicide adaptation. Sequence alignment analysis revealed that these mutants primarily mutated in ChSdhB and ChSdhD, with 3 genotypes: ChSdhBH277Y, ChSdhBI279T and ChSdhDH133Y, exhibiting two distinct categories of resistance: high resistance (HR) and moderate resistance (MR), which resistance factors (RF) is 214.22 and 44.33-53.67, respectively. These mutants were more pathogenic than the wild-type parental strains, but there was a significant reduction in mycelial growth rate and sporulation in the resistant mutants, indicating a significant fitness cost associated with resistance to Pyd. In addition, this study revealed a positive cross-resistance between Pyd and another SDHI fungicide cyclobutrifluram. However, no cross-resistance was found between Pyd and other classes of fungicide, including prochloraz, fludioxonil, iprodioneand pyraclostrobin. Homology modeling and molecular docking further confirmed that point mutation of ChSdhBH277Y, ChSdhBI279T, and ChSdhDH133Y could reduce binding affinity between Pyd and its target subunits from −74.07, −74.07, −152.52 kcal mol-1 to −3.90, −4.95, −9.93 kcal/mol, respectively. These findings not only provided valuable insights for managing SCLB caused by C. heterostrophus, but also enhanced our understanding of molecular mechanism underlying plant pathogen resistance to Pyd.

Metagenomic airborne resistome from urban hot spots through the One Health lens

AbstractHuman activities are a significant contributor to the spread of antibiotic resistance genes (ARGs), which pose a serious threat to human health. These ARGs can be transmitted through various pathways, including air, within the context of One Health. This study used metagenomics to monitor the resistomes in urban air from two critical locations: a wastewater treatment plant and a hospital, both indoor and outdoor. The presence of cell‐like structures was confirmed through fluorescence microscopy. The metagenomic analysis revealed a wide variety of ARGs and a high diversity of antibiotic‐resistant bacteria in the airborne particles collected. The wastewater treatment plant showed higher relative abundances with 32 ARG hits per Gb and m3, followed by the main entrance of the hospital (indoor) with ≈5 ARG hits per Gb and m3. The hospital entrance exhibited the highest ARG richness, with a total of 152 different ARGs classified into nine categories of antibiotic resistance. Common commensal and pathogenic bacteria carrying ARGs, such as Moraxella, Staphylococcus and Micrococcus, were detected in the indoor airborne particles of the hospital. Interestingly, no ARGs were shared among all the samples analysed, indicating a highly variable dynamic of airborne resistomes. Furthermore, the study found no ARGs in the airborne viral fractions analysed, suggesting that airborne viruses play a negligible role in the dissemination of ARGs.

Rethinking Resistance as Relational – Resisting Psychologization in Psychology: Lessons from Carrère’s Between Two Worlds

AbstractPsychology, and in particular mainstream positive psychology, is fuelled by discourses on resistance strategies, understood as the individual capacity to resist and adapt to negative and oppressive thoughts, circumstances, experiences, and social structures. This self-strategy of resistance is evident in positive psychology’s notions of resilience: grit, life-crafting, and job-crafting behaviors, for example. While positive psychology would have us believe these strategies are associated with overcoming hardship and living a good life, they risk imbricating people in their own oppressions. In this paper, we engage in a reading of Carrère’s Between Two Worlds (original title Ouistreham) (2021), a movie featuring multiple examples of resistance. The movie shows how precarious workers enact self-strategies of resistance to fight for a decent and bearable life. They persevere despite ongoing hardships, seek joy amidst tragic life events, and find meaning in menial labor. However, resistance also appears as relational and political, and thus escapes and exceeds self-focused psychological categories of resistance. Resistance appears as the refusal to be understood solely in individual and individualizing ways – as a psychologized and knowable subject – and is characterized by relational, contextual, and political tactics. The movie profanes established positive psychology’s individualist focus on resistance. This profanation of self-strategies of resistance affords an opportunity to rethink resistance beyond the individual and compels us to problematize the tendency to psychologize and individualize social phenomena. In doing so, our paper too resists the determination of psychological language (i.e., psychologization) and advances ideas for alternative resistances in, to, and of psychology.

Antimicrobial Resistance Profile of Uropathogenic Escherichia coli Isolated in Garaku, Keffi and Mararaban-Gurku Healthcentres, Nasarawa-West Senatorial District, Nasarawa State, Nigeria

Aims: This Study is aimed at studying the Antimicrobial Resistance profile of Uropathogenic Escherichia coli isolated in Garaku, Keffi and Mararaban-gurku Healthcentres, Nasarawa-West Senatorial District, Nasarawa State, Nigeria. Study Design: is a survey research. Place and Duration of Study: Department of Microbiology, Nasarawa State University, Keffi, between June, 2021 and January,2024. Methodology: 384 urine samples of patients with suspected cases of UTIs were collected and E. coli was isolated and identified using standard microbiological methods. Antimicrobial Susceptibility Testing of the isolates was carried out in accordance with the Clinical and Laboratory Standards Institute (CLSI) method. Results: The occurrence of E. coli was 92(24%). The isolates were generally more resistant to Ampicillin (AMP) with 75.0%. Streptomycin (S) 70.7% Cefotaxime (CTX) 68.5% but less resistant to Ciprofloxacin (9.8%) followed by Gentamicin 17.4%. The commonest resistance phenotype was AMC-AMP-CAZ-CTX-S-SXT (5.4%). Most of the isolates showed multiple antibiotic resistance (MAR) Index of above 0.2 with the commonest MAR Index at GHK, GHG and MCM-g of 0.7(21.4%), 0.4(28.0%) and 0.3(23.1%) respectively. The antibiotics resistant E. coli isolates were grouped into different categories of antibiotic resistance namely: Non Multiple Drug Resistance (NMDR); Excessive Drug Resistance (XDR); Multiple Drug Resistance (MDR) and Pan Drug Resistance (PDR). The occurrence of classes of antibiotic resistance was of the orderMDR75(81.5%) ˃ NMDR9(9.8%) ˃XDR7(7.6%) > PDR 1(1.1%). Conclusion: with the results from this research, E. coli being non susceptible to many antimicrobials is a clear indication that, prescription of these antimicrobials by physicians and usage by patients must be done with diligence and utmost care and vigorous campaign by stakeholders must be step up to bring to the barest minimum incidences of Antimicrobial resistance.

The Hidden World of Insect-Plant Interactions: a review

This review examines the hidden world of plant-insect interactions by emphasizing on the sensory perception and behavior of phytophagous insects, nutritional influence on insect reproduction, host plant resistance, Insects, weed and crop interaction, Insect pollinator plant interaction, tri-trophic interaction, and insect biotechnology. It explores how insects use sensory cues to forage for food, find mates, perceive dangers, and navigate their environment. It also examines the influence of host plants on insect behavior and the use of chemical cues for communication. The potential use of semio-chemicals in pest management for sustainable agriculture is highlighted. Nutritional factors and their impact on insect reproductive success are also discussed, emphasizing the need for balanced diets. The different categories of host plant resistance and their effects on insects are examined. The interdependent relationships between insects, plants, and weeds in agricultural ecosystems are explored, with a focus on the role of insect pollinators. The decline of insect pollinators and the importance of studying them are emphasized. The importance of tri-trophic interactions in maintaining ecological balance and biodiversity is discussed. Moreover, the role of biotechnological techniques like genomics, proteomics, transcriptomics, and epigenetics in understanding insect plant interactions as well as developing insect pest control strategies is discussed. The potential use of natural products produced by plants in environmentally friendly pest control methods is also examined. Overall, this review provides a comprehensive exploration of insect-plant interactions and the potential for sustainable pest control methods.

Requirements for cables as categories of construction products and thermal resistance of power cables

Introduction. One of the main driving factors of the demand for cable and conductor products is the modern trend of urbanization, which leads to an increase in cities with significantly increased requirements for electrical networks of transportation and distribution of electrical energy. This requires the development of appropriate infrastructure with significant demand for electricity in the commercial, industrial and residential sectors. The construction industry uses a wide range of cables with an appropriate set of electrical and mechanical properties, resistance to the influence of external climatic factors, and, first of all, must meet fire safety requirements. Increasing construction activity is stimulating the market for fire-resistant cables in the construction industry. According to the Construction Products Regulation, power, telecommunication cables, data cables, control and management, fiber optic refer to construction products in the EU member states. These cables are intended for the supply of electrical energy and communication, which are permanently installed in buildings and other engineering structures. Power, telecommunications, data and control cables are considered construction products, and are the only electrical products classified as construction. Over the next 15 years, Eastern Europe is expected to see increased growth in the construction industry, as the end of the war in Ukraine requires nearly $1 trillion in reconstruction. A strategic task in the reconstruction of the country is the use of cable and conductor products with increased operational properties, including in the construction industry. Significant efforts and investments in innovation and certification of cable and conductor products require manufacturers to create construction products of the «Power, control and communication cables» category with a high level of fire safety in accordance with the Euroclassification of cables in terms of reaction to fire depending on the level of safety. Purpose. Analysis of fire safety requirements for cables as a category of construction products and determination of thermal resistance of power cables based on experimental thermal studies of modern electrical insulating compositions. Methodology. The thermal stability of power cables with a voltage of 0,66/1 kV was determined, depending on the design, based on the conducted experimental studies of the thermal stability of electrical insulation materials, between the core filling and the polymer sheath, removed from the cable samples. On the basis of the conducted correlation analysis between thermal resistance and fire load, which are important parameters for confirming the quality and safety of the entire cable, it has been proven that the efficiency of halogen-free compositions to meet fire safety requirements increases with the increase in the cross-section and number of cores in the cable. Practical value. Determining the heat load and fire resistance of cables of various designs and areas of application based on the obtained experimental data on the heat of combustion of polymer cable materials is necessary and justified at the stage of mastering and determining the prospects for the production of cables with modern halogen-free electrical insulation compositions in accordance with fire safety requirements.

Феномен стійкості як здатності формувати ефективні методи подолання негативних екологічних впливів

The article discloses the psychological meaning of resilience, which includes meaningful life orientations that shape a person's adaptive potential. The purpose of the article is to study the phenomenon of resilience as the ability to form effective ways to overcome negative environmental impacts. The Connor-Davidson Stress Resistance Scale-10 (CD-RISC-10) was used among the psychodiagnostic methods of assessing stress resistance. The Bath Ankylosing Spondylitis Metrology Index (BASMI) and the Bath Ankylosing Spondylitis Functional Index (BASFI) were used to assess the functional status of respondents (trauma witnesses). The Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) was used to determine the activity of psychotrauma. To assess the quality of life, a specific questionnaire of the quality of life of the studied Ankylosing Spondylitis Quality of Life Questionnaire (ASQoL) was used. Vitality is ensured by a number of physiological, personal and social factors. The identification and specification of a person's characterological resources contribute to the determination of his resistance, personal potentials that would contribute to the preservation of a person's psychosomatic health, his According to the research results, we defined the categories of low stress resistance, which corresponded to 50 people (24 women and 26 men), moderate stress resistance was determined in 12 people, and the category of high stress resistance corresponded to 1 male respondent. We found certain gender differences in the stress resistance of the victims: it is higher in men. Respondents with high trauma were characterized by a significant loss of psychofunctional ability according to the BASFI scale. The presence of significant violations of psychofunctional ability was confirmed by the BASMI metrological index and the ASQoL quality of life questionnaire.

Threads of Tradition: Native American Women’s Resistance and Resilience in Piatote’s “Beading Lesson”

Resistance literature’s significance to empower the weak cannot be overemphasized. This article examines “Beading Lesson” by Native American (NA) women author, Beth Piatote. The short fiction highlights the importance of NA women’s roles within their communities. Utilizing the Indigenous feminist framework, this study applies seven analytical categories of resistance by Baaz et al. and integrates Southwick et al.’s concepts of resilience to examine how the narrator navigates through historical trauma and hardships. In the narrative, issues such as tumultuous relations with the U.S. government, exploitation, incarceration, cultural erasure, and change of gender roles, are brought up in the story through the light-toned monologue of an aunt, the narrator, who is teaching her niece how to bead. Contrasted to this backdrop of historical trauma and adversities is the narrator’s resistance and resilience embedded in her everyday actions. The findings revealed that the narrator employs discreet and non-confrontational forms of resistance demonstrated through routine activities such as beading, teaching, storytelling, and engaging in powwow rituals. These seemingly ordinary activities are actually powerful acts of resistance as they assert the narrator’s original gender role as keeper of tradition within the context of NA culture. The narrator’s resilience is firmly grounded in her strong sense of identity as an NA woman. By engaging in various forms of resistance, she not only challenges the oppressive system but also helps foster collective resilience in her community. As resistance literature, this short fiction reveals the multifaceted forms of resistance and resilience within NA communities.

Study on Mechanisms of Resistance to SDHI Fungicide Pydiflumetofen in Fusarium fujikuroi.

Rice bakaenii disease (RBD) is a widespread and devastating disease mainly caused by Fusarium fujikuroi. Pydiflumetofen (Pyd) is a novel succinate dehydrogenase inhibitor (SDHI) with strong inhibitory activity against F. fujikuroi, but the mechanism of resistance to Pyd has not been well studied for this pathogen. Through fungicide adaption, a total of 12 Pyd-resistant mutants were obtained and the resistance level could be divided into three categories of high resistance (HR), moderate resistance (MR), and low resistance (LR) with resistance factors (RF) of 184.04-672.90, 12.63-42.49, and <10, respectively. Seven genotypes of point mutations in FfSdh genes (FfSdhBH248L, FfSdhBH248D, FfSdhBH248Y, FfSdhC2A83V, FfSdhC2H144Y, FfSdhDS106F, and FfSdhDE166K) were found in these mutants, among which genotype FfSdhBH248L and FfSdhC2A83V mutants showed HR, genotype FfSdhBH248D, FfSdhBH248Y, FfSdhC2H144Y, and FfSdhDE166K mutants showed MR, and genotype FfSdhDS106F mutants showed LR. Moreover, all the substitutions of amino acid point mutations including FfSdhBH248L/D/Y, FfSdhC2A83V,H144Y, and FfSdhDS106F,E166K conferring resistance to Pyd in F. fujikuroi were verified by protoplast transformation. Additionally, a positive cross-resistance was detected between Pyd and another SDHI fungicide penflufen, while no cross-resistance was detected between Pyd and phenamacril, prochloraz, azoxystrobin, carbendazim, or fludioxonil. Although pathogenicity of the mutants was increased compared with that of the wild-type parental strains, the mycelial growth rate and spore production levels of the resistant mutants were significantly decreased (P < 0.05), indicating significant fitness cost of resistance to Pyd in F. fujikuroi. Taken together, the risk of resistance to Pyd in F. fujikuroi might be moderate, and appropriate precautions against resistance development in natural populations should be taken into account when Pyd is used for the control of RBD.

When subordinates do not follow: A typology of subordinate resistance as perceived by leaders

Whereas a plethora of research investigated subordinates who accept their leaders’ influence attempts (i.e., those who follow), we focus here on the reversed perspective, namely subordinates who decide not to follow their leaders’ requests. For example, a subordinate may intentionally lower their effort, regularly pass-off work tasks to colleagues, or take the leader for a fool. The purpose of the present study was to develop a conceptual account of this phenomenon. More specifically, we aimed to develop a classification of subordinate resistance as perceived by leaders that can guide future research on this multifaceted phenomenon. To achieve this goal, we conducted 40 semi-structured interviews with organizational leaders. We utilized topic modeling to map out five categories of subordinate resistance (i.e., entitlement, contact seeking/avoiding, effort minimization, emotionally fluctuating communication, and undermining team cohesion). In a second study (N = 1,229), we investigated the frequency with which leaders experienced each category of resistance, and explored leader-related antecedents (demographics, cognitive ability, personality) we well as situational antecedents (industry). We discuss a proposed nomological net of subordinate resistance, consequences that subordinates (and leaders) might face when engaging in (experiencing) subordinate resistance, and how the person–situation debate may contribute to understanding when subordinate resistance occurs.

Adorno and the categories of resistance

Adorno and the categories of resistance

INDIGENOUS URBANISMS

AbstractIndigenous urbanism is an analytic and vital experience that captures everyday life and extreme moments of conflict in settler colonies. While highly localized, Indigenous urbanism/s are comparable across time and space. Delivered from different parts of the world, the essays in this collection highlight that Indigenous urbanism is politically, socially and culturally significant not only for Indigenous peoples in cities, but also for urban settlers and non‐Indigenous people of color. While Indigenous urbanisms are foregrounded by settler‐colonial structures and processes, they also underscore the unresolved nature of social relations in cities, and indeed, the unsettled character of the city itself. This introduction briefly sketches the themes and scope of each essay and draws them into conversation. Taken together, this collection illustrates the relational—rather than reactionary—character of Indigenous urbanisms as structure, in and of the (settler) city. Indigenous urbanisms shape cities by engaging with broader categories of human relations, intimate connections, conflict and resistance.

Association Between Insulin Resistance and Cardiovascular Disease Risk Varies According to Glucose Tolerance Status: A Nationwide Prospective Cohort Study.

OBJECTIVETo investigate whether the association between insulin resistance and cardiovascular disease (CVD) differs by glucose tolerance status.RESEARCH DESIGN AND METHODSWe analyzed a nationwide sample of 111,576 adults without CVD at baseline, using data from the China Cardiometabolic Disease and Cancer Cohort Study. Insulin resistance was estimated by sex-specific HOMA of insulin resistance (HOMA-IR) quartiles for participants with normal glucose tolerance, prediabetes, or diabetes, separately, and by 1 SD of HOMA-IR for the overall study participants. We used Cox proportional hazards models to examine the association between insulin resistance and incident CVD according to glucose tolerance status and evaluate the CVD risk associated with the combined categories of insulin resistance and obesity in prediabetes and diabetes, as compared with normal glucose tolerance. Models were adjusted for age, sex, education attainment, alcohol drinking, smoking, physical activity, and diet quality.RESULTSIn participants with normal glucose tolerance, prediabetes, and diabetes defined by three glucose parameters, multivariable-adjusted hazard ratios (95% CIs) for incident CVD associated with the highest versus the lowest quartile of HOMA-IR were 1.03 (0.82–1.30), 1.23 (1.07–1.42), and 1.61 (1.30–2.00), respectively; the corresponding values for CVD per 1-SD increase in HOMA-IR were 1.04 (0.92–1.18), 1.12 (1.06–1.18), and 1.15 (1.09–1.21), respectively (P for interaction = 0.011). Compared with participants with normal glucose tolerance, in participants with prediabetes, the combination of the highest HOMA-IR quartile and obesity showed 17% (95% CI 2–34%) higher risk of CVD, while the combination of the lowest two HOMA-IR quartiles and nonobesity showed 15–17% lower risk of CVD. In participants with diabetes, the upper two HOMA-IR quartiles exhibited 44–77% higher risk of CVD, regardless of obesity status. Consistent findings were observed for glucose tolerance status defined by different combinations of glycemic parameters.CONCLUSIONSGlucose intolerance status exacerbated the association between insulin resistance and CVD risk. Compared with adults with normal glucose tolerance, adults with prediabetes who were both insulin resistant and obese exhibited higher risks of CVD, while in adults with diabetes, the CVD risk related to insulin resistance remained, regardless of obesity.

Why didn’t you resist? Situational influences on victim resistance during a rape

The purpose of this study is to examine factors influencing victim resistance during rape. Specifically, this study aims to understand which factors impact victim resistance using a multivariate approach focused on situational aspects related to offender, victim, and crime context characteristics. The sample includes 2,017 rape cases where victims did not resist, resisted passively, resisted verbally, or resisted physically. The first step of this study uses bivariate analyses to examine the relationship between the different categories of victim resistance and offender, victim, and crime context characteristics. Second, we computed three sequential binomial regressions in order to better understand the impact of each variable in multivariate modeling. The findings suggest that victim resistance is impacted by three main dimensions: victims’ physical and psychological vulnerabilities, the mentalizing of victimization risk, and the analysis of offenders’ vulnerabilities and additional risks to the victim. Both theoretical and practical implications for victims as well as for various actors in the criminal justice system are discussed.

Increased rates of extended-spectrum beta-lactamase isolates in patients hospitalized with culture-positive urinary Enterobacterales in the United States: 2011 – 2020

Antimicrobial resistance in Enterobacterales has made empiric therapy for hospitalized patients with urinary tract infections (UTIs) more challenging. We analyzed the antibiotic susceptibility of nonduplicate Enterobacterales isolates from urine cultures tested at US hospitals in the BD Insights Research Database (2011–2020). Multivariable generalized estimating equation models were used to assess resistance trends over time. A total of 322 US hospitals provided data on 876,507 urinary Enterobacterales isolates (62.4% Escherichia coli). Enterobacterales antibiotic resistance rates were 64.6%, 29.3%, 27.6%, and 26.3% for beta-lactams, fluoroquinolones, nitrofurantoin, and trimethoprim/sulfamethoxazole, respectively, and 12.4% had an extended-spectrum beta-lactamase (ESBL) phenotype. In multivariable models, rates of ESBL isolates and isolates resistant to ≥3 drug classes increased significantly between 2011 and 2020, while other categories of resistance generally decreased. We conclude that antimicrobial resistance is common in urinary Enterobacterales isolates. Management of UTIs should be guided by urine culture data and may benefit from new therapies.

Investigation of Metabolic Resistance to Soybean Aphid (Aphis glycines Matsumura) Feeding in Soybean Cultivars.

Simple SummaryThis project examined the interaction between soybean aphids and Ontario-grown soybean cultivars to determine which leaf metabolites were most associated with aphid resistance. Tolerance and resistance were determined by measuring the growth and reproduction of aphids and leaf feeding damage over 10-day and 4-week infestation periods. Chromatographic techniques were used for the analysis of legume-specific plant natural products and isoflavonoids, and high-resolution mass spectrometry was used for the identification of free amino acids in aphid-resistant, tolerant, and susceptible soybean cultivars. There was a low correlation between isoflavonoid leaf concentrations and aphid resistance in the soybean varieties studied; however, the aphid-resistant cultivars were determined to have lower free amino acid concentrations, indicating that lower nutrient quality could be responsible for the resistance observed. Identifying these cultivars is important for managing aphid populations and provides an additional tool for soybean integrated pest management.Soybean aphid (Aphis glycines) is a major soybean (Glycine max) herbivore pest in many soybean growing regions. High numbers of aphids on soybean can cause severe reductions in yield. The management of soybean aphids includes monitoring, insecticide applications when required, and the use of resistant cultivars. Soybean aphid-resistant soybean varieties are associated with genes that confer one or more categories of resistance to soybean aphids, including antibiosis (affects survival, growth, and fecundity), antixenosis (affects behaviour such as feeding), and tolerance (plant can withstand greater damage without economic loss). The genetic resistance of soybean to several herbivores has been associated with isoflavonoid phytoalexins; however, this correlation has not been observed in soybean varieties commonly grown in southern Ontario, Canada. Isoflavonoids in the leaves of 18 cultivars in the early growth stage were analyzed by HPLC and the concentration by fresh weight was used to rate the potential resistance to aphids. Greenhouse and growth cabinet trials determined that the cultivars with greater resistance to aphids were Harosoy 63 and OAC Avatar. The most susceptible cultivar was Maple Arrow, whereas Pagoda and Conrad were more tolerant to aphid feeding damage. Overall, there was a low correlation between the number of aphids per leaf, feeding damage, and leaf isoflavonoid levels. Metabolite profiling by high-resolution LC-MS determined that the most resistant cultivar had on average lower levels of certain free amino acids (Met, Tyr, and His) relative to the most susceptible cultivar. This suggests that within the tested cultivars, nutritional quality stimulates aphid feeding more than isoflavonoids negatively affect aphid feeding or growth. These findings provide a better understanding of soybean host plant resistance and suggest ways to improve soybean resistance to aphid feeding through the breeding or metabolic engineering of leaf metabolites.

A multicenter analysis of trends in resistance in urinary Enterobacterales isolates from ambulatory patients in the United States: 2011\u20132020

BackgroundUrinary tract infections (UTIs), which are usually caused by bacteria in the Enterobacterales family, are a common reason for outpatient visits. Appropriate empiric therapy for UTIs requires an understanding of antibiotic resistance in the community. In this nationwide study, we examined trends in antibiotic resistance in urinary Enterobacterales isolates from ambulatory patients in the United States (US).MethodsWe analyzed the antimicrobial susceptibility profiles (extended-spectrum beta-lactamase [ESBL]-producing phenotype and not susceptible [NS] to beta-lactams, trimethoprim/sulfamethoxazole [TMP/SMX], fluoroquinolones [FQ], or nitrofurantoin [NFT]) of 30-day non-duplicate Enterobacterales isolates from urine cultures tested at ambulatory centers in the BD Insights Research Database (2011–2020). The outcome of interest was the percentage of resistant isolates by pathogen and year. Multi-variable generalized estimating equation models were used to assess trends in resistance over time and by additional covariates.ResultsA total of 338 US facilities provided data for > 2.2 million urinary Enterobacterales isolates during the 10-year study. Almost three-quarters (72.8%) of Enterobacterales isolates were Escherichia coli. Overall unadjusted resistance rates in Enterobacterales isolates were 57.5%, 23.1%, 20.6%, and 20.2% for beta-lactams, TMP/SMX, FQ, and NFT, respectively, and 6.9% had an ESBL-producing phenotype. Resistance to two or more antibiotic classes occurred in 16.4% of isolates and 5.5% were resistant to three or more classes. Among isolates with an ESBL-producing phenotype, 70.1%, 59.9%, and 33.5% were NS to FQ, TMP/SMX, and NFT, respectively. In multivariable models, ESBL-producing and NFT NS Enterobacterales isolates increased significantly (both P < 0.001), while other categories of resistance decreased. High rates (≥ 50%) of beta-lactam and NFT resistance were observed in Klebsiella isolates and in non-E. coli, non-Klebsiella Enterobacterales isolates.ConclusionsAntimicrobial resistance was common in urinary Enterobacterales isolates. Isolates with an ESBL-producing phenotype increased by about 30% between 2011 and 2020, and significant increases were also observed in NFT NS Enterobacterales isolates. Resistance rates for all four antibiotic classes were higher than thresholds recommended for use as empiric therapy. Non-E. coli Enterobacterales isolates showed high levels of resistance to commonly used empiric antibiotics, including NFT. These data may help inform empiric therapy choices for outpatients with UTIs.

Noncoding RNAs in Drug Resistance of Gastrointestinal Stromal Tumor.

Gastrointestinal stromal tumor (GIST) is the most common mesenchymal tumor in the gastrointestinal tracts and a model for the targeted therapy of solid tumors because of the oncogenic driver mutations in KIT and PDGDRA genes, which could be effectively inhibited by the very first targeted agent, imatinib mesylate. Most of the GIST patients could benefit a lot from the targeted treatment of this receptor tyrosine kinase inhibitor. However, more than 50% of the patients developed resistance within 2 years after imatinib administration, limiting the long-term effect of imatinib. Noncoding RNAs (ncRNAs), the non-protein coding transcripts of human, were demonstrated to play pivotal roles in the resistance of various chemotherapy drugs. In this review, we summarized the mechanisms of how ncRNAs functioning on the drug resistance in GIST. During the drug resistance of GIST, there were five regulating mechanisms where the functions of ncRNAs concentrated: oxidative phosphorylation, autophagy, apoptosis, drug target changes, and some signaling pathways. Also, these effects of ncRNAs in drug resistance were divided into two aspects. How ncRNAs regulate drug resistance in GIST was further summarized according to ncRNA types, different drugs and categories of resistance. Moreover, clinical applications of these ncRNAs in GIST chemotherapies concentrated on the prognostic biomarkers and novel therapeutic targets.

An Expanded Framework for Community Viability Analysis

Abstract Community viability analysis (CVA) has been put forth as an analogue for population viability analysis (PVA), an accepted conservation tool for evaluating species-specific threat and management scenarios. The original proposal recommended that CVAs examine resistance-based questions. PVAs, however, are broadly applicable to multiple types of viability questions, suggesting that the original CVA definition may be too narrow. In the present article, we advance an expanded framework in which CVA includes any analysis assessing the status, threats, or management options of an ecological community. We discuss viability questions that can be investigated with CVA. We group those inquiries into categories of resistance, resilience, and persistence, and provide case studies for each. Finally, we broadly present the steps in a CVA.

Characterizing Resistance to Soybean Aphid (Hemiptera: Aphididae): Antibiosis and Antixenosis Assessment.

Host-plant resistance (HPR) remains a vital tool to manage soybean aphid (Aphis glycines Matsumura), a major pest of soybean in Midwestern United States and southern Canada. HPR can be overcome by virulent biotypes of A. glycines; thus, in order to increase the durability of resistant cultivars, HPR needs to be deployed strategically. To improve the strategic deployment, a complete understanding of HPR in existing resistant germplasm will help ensure HPR success. In this study, we characterized HPR soybean to determine antibiosis and antixenosis categories of resistance to different biotypes of A. glycines. No-choice and free-choice tests were performed on 11 previously reported plant introductions (PIs) possessing resistance to at least one A. glycines biotype (1, 2, and 3). Overall, we found that the PIs manifested differences of a particular resistance category in response to infestation by different biotypes. Our data from no-choice tests indicate that all tested PIs possess antibiosis-based resistance to three biotypes. However, the strength of antibiosis was variable as some PIs showed stronger antibiosis toward a given biotype than others. All tested PIs manifested antixenosis, in addition to antibiosis. Furthermore, detached leaf assays revealed that resistance to A. glycines was not retained in excised soybean leaves. Characterization of resistance in this study can contribute to develop strategies for future deployment of resistant cultivars developed from these PIs.