The present study investigated the combined effects of zofenoprilat (ZOFE) and nebivolol (NEBI) on endothelial function, focusing on their anti-inflammatory and antioxidant properties. The purpose was to evaluate whether these drugs, commonly used in clinical practice, offer a synergistic therapeutic strategy for managing hypertension and protecting vascular health. ZOFE, an ACE inhibitor, demonstrated significant anti-inflammatory activities by reducing inflammatory cytokines, thereby mitigating vascular inflammation, a key factor in hypertension and atherosclerosis. NEBI, a third-generation beta-blocker, exhibited strong antioxidant effects by enhancing nitric oxide (NO) levels, crucial for maintaining endothelial function and reducing oxidative stress. The potential effect of ZOFE and NEBI treatment was evaluated using human umbilical vein endothelial cells (HUVEC) as a model. Specifically, cells were challenged with tumor necrosis factor-α (TNF-α) to induce endothelial dysfunction. Subsequently, cell viability, NO production, protein levels of superoxide dismutase (SOD) and catalase (CAT), enzymatic activity of SOD and CAT, intracellular levels of glutathione (GSH), inflammatory status, and levels of interleukin-6 (IL-6) monocyte chemoattractant protein-1 (MCP-1), macrophage inhibitory cytokine-1 (MIC-1), and the active form of nuclear factor kappa B (p-NFκB), were analyzed. Our results showed that NEBI significantly counteracted oxidative stress, increasing the main antioxidant defenses (SOD, CAT, and GSH). The combination of ZOFE and NEBI resulted in a potentiated effect, enhancing both anti-inflammatory and antioxidant activities. This dual mechanism of action provides a comprehensive approach to protecting endothelial cells and improving vascular function. The combined therapy not only lowered blood pressure more effectively but also offered greater protection against endothelial damage compared to monotherapy with either drug alone. These findings suggest that the combination of ZOFE and NEBI could be particularly beneficial for patients with hypertension, especially those with coexisting inflammatory and oxidative stress-related conditions. This combination therapy, by addressing multiple pathogenic pathways simultaneously, could potentially be beneficial in patients with cardiovascular risk conditions. In conclusion, the combination of ZOFE and NEBI offers a potentially promising therapeutic approach for managing hypertension and protecting vascular health, aiming at improving clinical outcomes for patients with cardiovascular diseases. Not applicable.

Bifidobacterium supplementation ameliorates LPS-induced immune stress and intestinal barrier dysfunction in goslings.

Oxidative stress and immune responses in whiteleg shrimp (Penaeus vannamei) under ozone nanobubbles exposure.

Biochemical effects and theoretical study of a metformin in the carp (Cyprinus carpio) fry.

Oral delivery of engineered Lactococcus lactis microcapsules expressing antioxidant enzymes for inflammatory bowel disease: Mechanisms of intestinal barrier repair and microbiota modulation.

Natural supplements effectively suppress Helicobacter pylori colonization and improve gastric antioxidant and antiinflammatory responses in an indomethacin-induced ulcer model.

Plant growth-defense trade-offs regulate phytoremediation efficiency and ecosystem resilience in the contaminated environments.

The antioxidant function of Piper sarmentosum Roxb. alcoholic extract in heat-stressed broilers.

Systematic pharmacology-guided analysis of the therapeutic mechanism of Sedum sarmentosum against chronic liver injury.

Chemical basis for the antioxidant activity of Piper nigrum: integrative profiling of volatile components from multi-origin black and white pepper.

Electrochemical sensor analysis of catalase (CAT) in delaying skeletal muscle aging during endurance exercise: GLUT4 protein molecule improves glucose and lipid metabolism

Oral drug therapy for inflammatory bowel disease (IBD) is often hindered by inadequate targeting, low bioavailability, and reactive oxygen species (ROS) accumulation. To address these challenges, we have developed hydrogel microspheres@Cu-Mn3O4 nanozymes (HMCM) by encapsulating hydrothermally synthesized Cu-doped Mn3O4 nanozymes (CM NZs) into calcium alginate hydrogel microspheres (HM) using microfluidics. These microspheres are designed for colon-targeted IBD treatment. The CM NZs exhibit exceptional superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, effectively scavenging ROS such as H2O2, ·OH, and O2 -·. The negatively charged HMCM promotes targeted accumulation in inflamed colon regions and facilitates specific nanozyme release. In a dextran sulfate sodium (DSS)-induced colitis mouse model, HMCM administration enhanced the expression of tight junction proteins (Claudin, ZO-1, Occludin) and repaired the damaged intestinal barrier. The oral HMCM group significantly reduced inflammation, enhanced antioxidant activity, and inhibited ferroptosis in colonic tissue through the upregulation of GPX4 and SLC7A11. It also restored gut microbiota balance by increasing probiotic populations and suppressing harmful bacteria. Systemic biosafety assessments confirmed HMCM's colon-specific retention and high biocompatibility. This research establishes HMCM as a precision-targeted colonic drug delivery platform, offering a promising therapeutic strategy for the treatment of inflammatory bowel disease.

Physiological and genomic insights into the salt tolerance mechanisms of three Staphylococcus strains isolated from moromi.

Selenium modulates the antioxidative response in green microalga Monoraphidium cf. contortum.

Demethyleneberberine attenuates combined cognitive and metabolic dysfunctions in an insulin-resistance-induced Alzheimer's disease rat model: Synthesis, in-silico and in-vivo insights.

Evaluation of embryotoxicity and teratogenicity of Cenostigma pyramidale (Tul.) fruit extract in zebrafish (Danio rerio).

Pharmacological potential of aqueous extract of Ononis natrix in mitigating early-life lead toxicity: behavioral, biochemical, histological, and immunohistochemical evaluation.

The protective effect and mechanism of phycocyanin on diabetic retinopathy in rats.

Structural characterization and antioxidant activity evaluation of a polysaccharide obtained from the wheat aleurone layer fermented by Agrocybe aegerita.

Effects of short-term exposure to ferrous sulfate on bioaccumulation, oxidative stress biomarkers, immunity, and intestinal microbiota in Litopenaeus vannamei.