Thermoplastic starch has been attracted due to its availability, biodegradability, and processability. However, its poor tensile properties, high hydrophilicity and lack of antibacterial activity are still the major concerns for many applications. In current study, several thermoplastic cassava starch samples (THPCS), reinforced with kapok (KP) fibers and modified by varying levels of tartaric acid (TA), a natural organic acid, were prepared to enhance their limitations. All samples were mixed and shaped using an internal mixer and a compression molding machine, respectively. The modified THPCS samples were characterized using analytical techniques such as FTIR, SEM and TGA. Morphology, water uptake, mechanical properties, biodegradability, and antimicrobial performance of various samples were also evaluated. IR peak positions of O–H stretching as well as O–H bending were clearly observed for shifting to lower wavenumbers with the addition of KP fibers or KP fibers/TA, indicating of new H-bond formation. In addition, the use of KP fibers caused the enhancement of mechanical strength and thermal properties including the reduction of water uptake. Moreover, THPCS/KP fiber sample modified by TA presented surface smoothness, good biodegradability, and antibacterial performance against both Staphylococcus aureus and Escherichia coli.