AbstractPurpose To show the use of anterior segment optical coherence tomography (AS‐OCT) in conjunctival, limbal, subconjunctival tumors and to compare the data with histopathologic specimens.Methods 15 patients (15 eyes) with conjunctival, limbal and subconjunctival lesions were examined using AS‐OCT (Visante OCT 2.0, Carl Zeiss Meditec, Dublin, CA). Eyes were scanned using Anterior Segment Single, High Resolution Corneal and Raw Image modes. Gray‐scale, OCT Color and Rainbow Color images were analyzed using built‐in software. All tumors were excised and histopathologicaly examined.Results In all small tumor cases AS‐OCT allowed to see the deeper lesion structure with complete penetration. In conjunctival melanoma, papilloma, subepithelial naevus and pterygia cases AS‐OCT showed tumor structure, layers and extension clearly. Underlying cornea was also visible. In larger and solid tumors (squamous cell carcinoma, fibroma and lipoma) anterior surfaces were hyper‐reflective, but the deeper structures of the lesions were incompletely penetrated. However, in all cases it was possible to differentiate cystic lesions from solid lesions. Histopathologic examination revealed the diagnosis and in small tumors confirmed the AS‐OCT data showing tumor epithelium and underling layers.Conclusion AS‐OCT may be a useful non‐invasive diagnostic tool for the evaluation of ocular surface tumors in selective cases. AS‐OCT may give useful information before planning the surgery.