Cases Of High-grade Lesion Research Articles

Socio-demographic Characteristics and Incidence of CIN of Uterine Cervix among VIA Positive Women attended at a Tertiary Care Hospital in Dhaka City

Background: Women in different socio-demographic are presented with VIA positivity.
 Objective: The purpose of the present study was to see the socio-demographic characteristics and incidence of CIN of uterine cervix among of VIA positive women.
 Methodology: This cross sectional study was conducted in the Department Of Pathology at Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during the period of July 2007 to June 2008 for one (01) year. Patients who were colposcopically positive for cervical intraepithelial neoplasia and early invasive carcinoma or patients who are colposcopically negative but clinically suspicious for cervical neoplasia were included in this study. The clinical history was obtained by taking history with particular attention to age, age at marriage, parity, history of contraceptives, abnormal per vaginal discharge and post coital bleeding. The cervix was examined on naked eye by Cusco’s speculum to see whether it was healthy or not. Then it was examined by the colposcope after applying 3.0 to 5.0% acetic acid and colposcopic findings were collected. VIA positive cases were underwent colposcopy guided LEEP biopsy.
 Result: A total of 63 patients of different age group were recruited for this study. The mean with SD of age of this study population was 34.6±9.59 years. In this study, 41(65%) cases were diagnosed as cervical intraepithelial neoplasia (CIN). Incidence of CIN-I was 10 cases in age group 20 to 29 years. Most of the cases were in 30 to 39 (18 cases) years age group. Among 20 cases of high grade lesion (CIN-II/III) 9(45%) cases were in lower class, 8(40%) cases were in middle class and 3(15%) cases in upper class. Conclusion: In conclusion majority of the women were middle aged reproductive age group who are mostly in the lower class of socioeconomic class.
 Journal of Current and Advance Medical Research 2020;7(1): 7-11

PAX1 Methylation Analysis by MS-HRM is Useful in Triage of High-grade Squamous Intraepithelial Lesions

This study is aimed to investigate the role of paired boxed gene 1 (PAX1) methylation analysis by methylation- sensitive high-resolution melting (MS-HRM) in the detection of high grade lesions in atypical squamous cells cannot exclude high-grade squamous intraepithelial lesion (ASC-H) and compared its performance with the Hybrid Capture 2 (HC2) human papillomavirus (HPV) test. In our study, 130 cases with a diagnosis of ASC-H from the cervical cytological screening by Thinprep cytologic test (TCT) technique were selected for triage. Their cervical scrapings were collected and evaluated by using PAX1 methylation analysis (MS-HRM) and high-risk HPV DNA test (HC2), followed by colposcopy and cervical biopsy. Chi-square test were used to test the differences of PAX1 methylation or HPV infection between groups. In the detection of CIN2+, the sensitivity, specificity, the PPV, NPV and the accuracy of PAX1 MS-HRM assay and high-risk HPV (HR-HPV) tests were respectively 80.6% vs 67.7%, 94.9% vs 54.5%, 83.3%, vs 31.8%, 94.0% vs 84.4%, and 91.5% vs 57.7%. The PAX1 MS-HRM assay proved superior to HR-HPV testing in the detection of high grade lesions (CIN2+) in ASC-H. This approach could screen out the majority of high grade lesion cases of ASC-H, and thus could reduce the referral rate to colposcopy.

Study of quantitative detection of circulating DNA in the plasma of patients with cervical lesion

To quantitatively detect circulating DNA levels in the plasma of patients withcervical lesion and to determine the value for diagnosis of cervical lesion and cervical cancer. Preoperative blood samples were collected from 53 cases of low-grade lesions, 49 cases of high-grade lesions, 44 cases of cervical invasive cancer and 70 cases of healthy women. Plasma DNA was extracted by magnetic bead method (BILATEST DNA kit). The quantity of plasma DNA was determined by duplex real-time quantitative PCR. Median plasma DNA level of invasive cervical cancer patients was 61.59 mg/L (32.06-162.16 mg/L), which was significantly higher than that of healthy women [16.35 mg/L (11.98-22.71 mg/L), P<0.01]. Among invasive cervical cancer patients, median plasma DNA level of squamous carcinoma patients was slightly higher than that of adenocarcinoma (50.43 versus 47.31 mg/L, P>0.05). Median plasma DNA level of stage I patients was lower than that of stage II-III patients (46.02 versus 71.35 mg/L, P<0.05). Quantitatively detecting plasma circulating DNA may be with some application prospect in the diagnosis of cervical diseases.