Casein Phosphopeptide-amorphous Calcium Phosphate Research Articles

Maintenance of dental bleaching: A systematic review

After bleaching procedure, color pigments can adhere to the rough dental surface and can trigger more discoloration. In this sense, no standard post-bleaching maintenance technique exists. The purpose of this study was to evaluate the maintenance of enamel color after bleaching procedure through a systematic review. The research question was defined as follows: Is the tooth whitening maintenance necessary after a bleaching treatment? Two reviewers conducted the literature search in PubMed (MEDLINE), Web of Science, Scopus, EMBASE, and SciELO. Both in vitro and clinical trials reporting the color change of bleached enamel after the use of a tooth whitening agent were included in the review. The risk of bias in the included manuscripts was evaluated using the RoBDEMAT tool. A total of 11 articles were included in the qualitative analysis. Due to the lack of groups for comparison, a meta-analysis could not be conducted. The whitening maintenance procedures identified were remineralizing agents, antioxidants, over-the-counter agents, toothpaste containing Blue Covarine, casein phosphopeptide-amorphous calcium phosphate fluoride paste, 0.05% sodium fluoride mouthwash, arginine, nano-hydroxyapatite, nano-carbonate apatite, and CO2 laser. In conclusion, tooth whitening maintenance could be achieved by using some agents. The association of these agents might be beneficial for maintaining the color of the enamel throughout 12 months following bleaching procedure.

Comparative Evaluation of Ion Release and Uptake of Fluoride Varnish Containing Dicalcium Phosphate Dihydrate and Casein Phosphopeptide-Amorphous Calcium Phosphate – An Experimental Approach.

The effectiveness of fluoride varnish may be restricted due to inadequate bioavailability of phosphate and calcium ions in the oral cavity. Dicalcium phosphate dihydrate and Casein phosphopeptide–amorphous calcium phosphate have exhibited ability to enhance absorption of calcium and fluoride. The present study aims to compare the remineralization potential of fluoride varnishes containing DCPD and CPP-ACP. Materials and Method: The ion release, precipitation and surface microhardness of enamel blocks were compared following topical application of various Floride varnishes. Thirty enamel blocks were divided into 3 groups following randomization that included: NaF varnish (Group A), CPP-ACP NaF varnish (Group B) and DCPD- NaF varnish (Group C). Pre- and post-treatment microhardness was evaluated using Vickers Microhardness Tester. UV-Vis Spectroscopy assessed the fluoride release at 2 hrs, 4 hrs and 6 hrs. The uptake of fluoride, calcium and phosphates were determined using SEM-EDX. The statistical significance was tested with One-way ANOVA variance for normality followed by post-hoc tests (p ≤ 0.05). Results: All the groups showed statistically significant increase in microhardness post-treatment (Group A: p=0.03, Group B, C: p=0.00). The mean increase in VHN was Group B (53.67) > Group C (34.75) > Group A (27.40). No significant difference in the F release was noted over time (p=0.00). The fluoride release over all three time intervals was Group C > Group B > Group C. Fluoride, Calcium and Phosphates showed highly significant differences between groups (p=0.00) with Group C > Group A> Group B. Conclusion: The novel NaF-DCPD Varnish displayed a promising remineralization potential, however it cannot be conclusively considered to be superior to the commercially available NaF-CPP ACP Varnish. Further in-vivo investigations need to be undertaken to determine the efficacy of NaF-DCPD varnish to determine its feasibility in clinical practice.

Efficacy of Sodium Fluoride and Fluoridated Calcium Phosphate in Mitigating Dental Erosion on Human Enamel: An In Vitro Analysis.

With increasing prevalence of dental erosion, this study explores the protective role of traditional fluoride-based products and newer formulations on eroded enamel. To assess the protective effectiveness of casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) and sodium fluoride (NaF) on human enamel against erosion using surface microhardness (SMH) and Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) analyses. Ten extracted human third molars were sectioned to obtain 40 enamel sections and randomly assigned into four groups (n = 10) and treated as follows: G1 (Sound Enamel), G2 (Erosive Challenge), G3 (CPP-ACPF + Erosive Challenge), and G4 (NaF + Erosive Challenge). All samples were subjected to Vicker's SMH analysis, while changes in surface morphology and elemental composition were validated in few representative samples using FTIR and SEM, respectively. Paired samples test, Kruskal-Wallis test, and Tukey HSD test were performed using SPSS software version 23 setting P value < 0.05 as statistically significant. The mean SMH1 values for the experimental groups G3 and G4 were significantly higher (426.58VHN and 455.83VHN) when compared to G1 (P = 0.000) and G2 (P = 0.000). In SEM analysis, G2 showed eroded honeycomb appearance compared to the smooth homogenous surface of G1, while both G3 and G4 showed deposition of some precipitates. FTIR analysis revealed that in G3 and G4, a characteristic peak of phosphate vibrations between 528 and 823 cm-1 and carbonate bands at 845-932 cm-1 was observed. Both CPP-ACPF and NaF demonstrated a protective effect on enamel against erosive challenge by an orange juice-based beverage.

Biofilm modulation and demineralization reduction after treatment with a new toothpaste formulation containing fluoride, casein phosphopeptide-amorphous calcium phosphate, and sodium trimetaphosphate: In situ study

ObjectiveThis in situ study aimed to evaluate a new toothpaste formulation containing fluoride (F), casein phosphopeptide amorphous calcium phosphate (CPP-ACP) and sodium trimetaphosphate (TMP) on the process of dental demineralization and biofilm composition. MethodsThis crossover double-blind study consisted of five phases, in which 10 volunteers wore intraoral appliances containing four bovine enamel specimens. The cariogenic challenge was performed using 30 % sucrose solution. Blocks were treated 3 ×/day with the following toothpastes: 1) Placebo (No F-TMP-CPP-ACP), 2) 1100 ppm F (1100F), 3) 1100F + 3 %TMP (1100F-TMP), 4) 1100F + 10 %CPP-ACP (1100F-CPP-ACP) and 5) 1100F-CPP-ACP-TMP. After 7 days, the percentage loss of surface hardness (%SH), integrated loss of subsurface hardness (ΔKHN), F, calcium (Ca) and phosphorus (P) concentration in the enamel was determined. The concentration of F, Ca, P and insoluble extracellular polysaccharide (EPS) in the biofilm were analyzed. ResultsThe addition of CPP-ACP-TMP to 1100F reduced %SH by 42 % and 39 % when compared to the 1100F and 1100F-CPP-ACP (p < 0.001); in addition, to a reduction in lesion body (ΔKHN) by 36 % for the same treatments. The treatment with 1100F-CPP-ACP-TMP led to a significant increase in the concentration of F, P and Ca in the enamel and biofilm, and reduced the concentration of EPS (p < 0.001). SignificanceToothpaste formulation containing 1100F-CPP-ACP-TMP prevented the reduction of enamel hardness and significantly influenced the ionic biochemical composition and insoluble extracellular polysaccharide (EPS) in biofilm formed in situ. These results are promising and provide valuable insights for the design of further clinical trials.

Remineralization Effect of Bioactive Glass With and Without Fluoride and Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) on Artificial Dentine Caries: An In Vitro Study.

Background/objectives Dentine hypersensitivity (DH) is a common dental condition marked by transient, sharp pain arising from dentinal exposure. Bioactive materials are capable of remineralization. This study aims to explore the remineralization effect of bioactive glass (BAG) with and without fluoride and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), using dentine discs as the test substrate. Materials and methods In this in vitro experimental study, 28 dentine discs were prepared from premolar teeth. Artificial caries were induced by subjecting the dentine discs to demineralization in acid for 72 hours. After demineralization, the discs were treated with various remineralizing dentifrices to evaluate their effects. The dentine discs (n=28) were divided into four groups: group 1 = BioMin (BioMin Technologies, London, United Kingdom), group 2 = NovaMin/Sensodyne Repair (GlaxoSmithKline plc, London, England, United Kingdom), group 3 = Recaldent (CPP-ACP; GC Orthodontics Inc., Alsip, Illinois, United States), and group 4 = deionized water (control group). All discs underwent a 28-day remineralization process using the respective dentifrices assigned to each group. The microhardness of the discs was measured using Vickers microhardness testing at three stages: baseline, post-demineralization, and post-remineralization. Results There was no significant difference in terms of microhardness between groups both at the baseline (F (3, 24) = 1.079, p = 0.995) and after the demineralization process (F (3, 24) = 1.310, p = 0.294). However, a significant difference was identified between the groups after the remineralization process (F (3, 24) = 34.008, p = 0.001). Additionally, the least significant difference (LSD) multiple comparison test was performed. There were significant differences identified in the remineralization condition, with group 1 having the highest values, followed by group 2, group 3, and group 4. Conclusion The results indicate that the remineralization effect on artificially induced dentine caries was more pronounced with bioactive glass containing fluoride compared to bioactive glass without fluoride and CPP-ACP.

Remineralizing Effect and Ion Release of Pearl Powder versus Casein Phosphopeptide Amorphous Calcium Phosphate on Non-cavitated Initial Enamel Lesions: An In Vitro Study

Remineralizing Effect and Ion Release of Pearl Powder versus Casein Phosphopeptide Amorphous Calcium Phosphate on Non-cavitated Initial Enamel Lesions: An In Vitro Study

Preventing Dental Caries with Calcium-Based Materials: A Concise Review

This concise review provides an update on the use of calcium-based materials for the prevention of dental caries. Some calcium-based materials promote remineralization and neutralize bacterial acids, disrupting cariogenic biofilms and inhibiting bacterial growth. Medical Subject Headings of [Dental Caries] and [Calcium] were adopted to search publications. Information related to the aim of this review was extracted and summarized. Common calcium-based materials are calcium phosphate, hydroxyapatite, calcium carbonate, calcium fluoride and casein phosphopeptide–amorphous calcium phosphate (CPP-ACP). Calcium phosphate is commonly used in toothpaste. It provides calcium and phosphate ions, enhances the incorporation of fluoride into caries lesions and increases mineral density. Hydroxyapatite is a form of calcium phosphate that is chemically similar to the mineral found in teeth. It can be applied on teeth to prevent caries. Calcium carbonate can be found in toothpastes. It neutralizes bacterial acids and acts as a calcium reservoir during remineralization. Calcium fluoride is found in dental products and promotes remineralization as a source of fluoride, which can be incorporated into tooth enamel, forming fluorapatite and increasing resistance to caries. CPP-ACP is derived from milk proteins. It contains calcium and phosphate, which help to remineralize tooth enamel. CPP-ACP inhibits cariogenic bacteria. It also interacts with bacterial biofilms and disrupts their formation. These calcium-based materials can be used to boost the preventive effect of fluorides or, alternatively, as a therapy for caries prevention.

Molar Incisor Hypomineralization: Prevalence and Treatment Needs in 7- to 9-year-old Children of Lucknow City.

Molar incisor hypomineralization (MIH) is one of the most common developmental disturbances that dental practitioners encounter, which may influence the child's quality of life and can impact their future dental health. To determine prevalence and treatment needs of MIH in children of Lucknow. A total of 800 children aged 7 to 9 years were clinically screened for the presence of MIH. All demographic details were filled in by the examiner in communication with the parents, and examination was performed using the Würzburg MIH concept. The overall prevalence of MIH in the children examined was 5.12%. A higher prevalence was found in males (7%) than in females (3.25%). The distribution of MIH was higher in the mandibular arch than in the maxillary arch. Mandibular molars were the most affected teeth, followed by maxillary incisors, and the least affected teeth were maxillary molars. On the basis of severity, 55.14% of teeth with MIH had no breakdown or hypersensitivity followed by 30.14% of teeth with hypersensitivity but no breakdown, and 7.35% of teeth had both hypersensitivity and breakdown. Based on severity, in 39.70% of teeth, the intervention suggested was fluoridated toothpaste, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) remineralizing agent, and varnish application. This was followed by the application of sealants and low-viscosity glass ionomer cement (GIC) in 38.97% of teeth, and in 7.35% of teeth, short- and long-term restorations, including stainless steel crown (SSC), were recommended. Prevalence of 5.12% was observed in children of Lucknow city. The characteristics of MIH defects were predictive of the treatment of the affected first permanent molars and incisors and can guide their management. Chowdhury AR, Singh N, Rathore M. Molar Incisor Hypomineralization: Prevalence and Treatment Needs in 7- to 9-year-old Children of Lucknow City. Int J Clin Pediatr Dent 2024;17(7):790-795.

In Vitro Efficacy of Tricalcium Phosphate and Casein Phosphopeptide Amorphous Calcium Phosphate Fluoride for Remineralization of Enamel White Spot Lesions.

Objectives: The main purpose of this study was to compare the remineralizing effects of casein phosphopeptide amorphous calcium phosphate fluoride (CPP-ACPF) and tricalcium phosphate (TCP) on artificially induced enamel white spot lesions (WSLs). Materials and Methods: In this in vitro study, 45 sound extracted premolars were immersed in a demineralizing solution (pH=4.5) for 96 hours, and were randomly divided into 3 groups of TCP, MI Paste Plus, and control. They were exposed to the remineralizing agents for 5 minutes once a day for 30 days. After mounting the teeth in resin blocks and polishing, they underwent a microhardness test at 3 different depths from the enamel surface. Data were analyzed by Prism software, two-way ANOVA, and Tukey's test (α=0.05). Results: The volume percentage of mineral content (VPM) was significantly different among the three groups at 30-, 60- and 90µm depths (P<0.0001). At 30µm depth, CPP-ACPF was significantly more effective than TCP (P<0.0001). At 60- and 90µm depths, there was no significant difference between CPP-ACPF and TCP (P>0.05). Conclusion: Both CPP-ACPF and TCP had significant efficacy for remineralization of artificially induced enamel WSLs under in vitro conditions.

Evaluation of the efficacy of various remineralization agents and grape seed extract on microhardness and lesion depth of primary tooth enamel: An in vitro study.

This study evaluated the efficacy of grape seed extract (GSE) on the remineralization of primary tooth enamel alone or in combination with remineralizing agents. The initial microhardness value of 90 primary tooth enamel samples was calculated; then, the samples were demineralized. The post-demineralization hardness of the samples was measured and the samples were randomly divided into 6 groups as follows: G1: negative control, G2: GSE, G3: NaF, G4:Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), G5: GSE+NaF, and G6: GSE+CPP-ACP (n=15). Oral environment pH cycle was applied and hardness measurements were repeated after treatments. The samples were stained with 1% rhodamine B dye and sectioned, and the lesion depth was measured. Statistical significance was set at P<0.05. The hardness decrease of the GSE and GSE+NaF groups was less than the other groups (P<0.05). The decrease was also less in the other groups than in the control group (P>0.05). GSE showed a positive effect when combined with NaF in maintaining microhardness but did not show the same effect when combined with CPP-ACP (P<0.05). Concerning penetration depth, all the groups had statistically lower values than the control group (P<0.05). The lowest penetration rates were observed in the GSE+NaF and GSE+CPP-ACP groups (P<0.05). The lowest hardness decrease was observed in the GSE and GSE+NaF groups, and the lowest penetration rates were observed in the GSE+NaF and GSE+CPP-ACP groups. It has been determined that a 15% GSE solution might be used as an alternative to fluoride in primary tooth remineralization and can increase the effectiveness of fluoride when used together.

Nanotechnology and Its Application in Dentistry: A Systematic Review of Recent Advances and Innovations.

Background: This study looks at the clinical applications of nanotechnology in dentistry, with an emphasis on implantology, preventive care, orthodontics, restorative dentistry, and endodontics. Methods: Following PRISMA criteria and registered in PROSPERO (ID: CRD 564245), a PubMed, Scopus, and Web of Science search was conducted for studies from January 2014 to April 2024. The criteria were English-language research on nanotechnology in dental coatings, with a focus on clinical trials and observational studies. The electronic database search yielded 8881 publications. Following the screening process, 17 records were selected for qualitative analysis. Results: Nanotechnology has revolutionized dentistry. In orthodontics, nanoparticles improve antibacterial characteristics, durability, and biocompatibility, lowering bacterial colonization and plaque. In preventative care, Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) combined with stannous fluoride (SnF2) and nano-sized sodium trimetaphosphate (TMPnano) substantially remineralizes enamel. Nanostructured surfaces in dental implants, particularly those containing calcium, improve osseointegration and stability. Nanoparticles in restorative dentistry improve composite and adhesive strength, aesthetics, and longevity. Conclusions: Nanotechnology improves dental materials and equipment, resulting in better treatment outcomes and increased patient comfort. Its integration provides more effective treatments, which improves dental care and patient outcomes. More research is needed to overcome present problems and expand nanotechnology's medicinal applications.

Casein phosphopeptide-amorphous calcium phosphate varnish versus dentin desensitizer in the treatment of non-carious dentin hypersensitivity

Background: Dentin hypersensitivity (DH) is one of the most common conditions clinicians encounter in clinical practice. New techniques are continuously being set forth to treat this condition. In recent times, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) has garnered attention owing to its ability to improve remineralization and prevent enamel demineralization. Consequently, it has been indicated as an advanced treatment for DH. Purpose: This clinical trial aims to assess the efficacy of a CPP-ACP varnish in the management of non-carious cervical hypersensitivity and compare it with that of a dentin desensitizer. Methods: Patients between the ages of 20 and 65 years who reported to our institute with complaints of DH were selected for this study. Forty teeth were chosen for this study and randomly assigned to two groups. The test group was treated with a CPP-ACP varnish (MI Varnish®, GC Corporation, Japan), while the control group was treated with a dentin desensitizer (Gluma®, Heraeus-Kulzer, Germany). Dentin hypersensitivity assessments were conducted during pre-treatment, immediately following treatment, and 2 and 4 weeks after treatment. Statistical analysis was performed after data collection. Results: An intragroup comparison showed both the CPP-ACP varnish and the dentin desensitizer achieved a maximum decrease in sensitivity in the time interval from baseline to post-op, and these desensitizing effects were sustained through the second and fourth week following treatment. An intergroup comparison revealed no statistically significant difference in sensitivity between the two groups at different time intervals. Conclusion: It could be concluded that CPP-ACP varnish and dentin desensitizer possess similar efficacy in the treatment of DH.

A comparative evaluation of human enamel remineralization ability of biomimetic nacre against casein phosphopeptide-amorphous calcium phosphate: An in vitro study.

This study aimed to assess and compare the efficacy of Nacre and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on the remineralization of enamel using surface microhardness analysis, scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy. Twenty human maxillary premolars extracted for orthodontic reasons were collected. Under cool water spray, the crowns were sectioned mesiodistally into buccal and palatal halves using a diamond disc. The samples were subsequently mounted in self-cure acrylic resin. The samples were then subjected to Vickers hardness testing and SEM-EDX for baseline. To simulate carious lesions, all of the samples were acid-etched with 37% phosphoric acid for 30 s in a specific area on the enamel samples and subjected to surface microhardness testing and SEM-EDX. The enamel samples were randomly assigned to Group 1: Nacre water-soluble matrix (WSM), Group 2: Nacre varnish, and Group 3: CPP-ACP for remineralization. After 21 days, remineralization assessment of the test samples was done using SMH analysis and SEM-EDX analysis. Data obtained were statistically analyzed using the one-way analysis of variance to reveal the significant differences between the groups. Tukey's test was used for post hoc comparisons. All three groups showed a significant increase in surface microhardness. All three groups showed a significant calcium and phosphorous ratio increase after remineralization. Among the three groups, the highest Ca:P ratio was seen in the Nacre WSM group (0.58) followed by the Nacre Varnish (0.57) and CPP-ACP group (0.57). SEM images of the Nacre surface revealed the presence of extensive interlocking. A layer of packed hydroxyapatite particles was formed on the surface of the nacre through surface reactions. All the groups in the present study showed some extent of remineralizing ability irrespective of the different materials and mechanisms of action. Nacre WSM showed a remarkable hardness spike close to natural enamel after demineralization.

Comparative Evaluation of Tooth Enamel Surface Microhardness Exposed to Novel Modified Casein Phosphopeptide Amorphous Calcium Phosphate, APF Gel and GC Tooth Mousse

Comparative Evaluation of Tooth Enamel Surface Microhardness Exposed to Novel Modified Casein Phosphopeptide Amorphous Calcium Phosphate, APF Gel and GC Tooth Mousse

Effects of graphene oxide and graphene quantum dots on enhancing CPP-ACP anti-caries ability of enamel lesion in a biofilm-challenged environment

ObjectiveTo investigate the anticaries effects of graphene oxide (GO) and graphene quantum dots (GQDs) combined with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on enamel in a biofilm-challenged environment. Material and methodsGO and GQDs were synthesised using citric acid. The antibiofilm and biofilm inhibition effects for Streptococcus mutans were evaluated by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming units (CFU). Remineralisation ability was determined by assessing mineral loss, calcium-to-phosphorus ratio, and surface morphology. To create a biofilm-challenged environment, enamel blocks were immersed in S. mutans to create the lesion and then subjected to artificial saliva/biofilm cycling for 7 days. Anticaries effects of GO, GQDs, GQDs@CPP-ACP, GO@CPP-ACP, and CPP-ACP were determined by broth pH and mineral changes after 7-day pH cycling. Biocompatibility was tested using a Cell Counting Kit-8 (CCK8) assay for human gingival fibroblasts (HGF-1). ResultsGQDs and GO presented significant antibiofilm and biofilm inhibition effects compared to the CPP-ACP and control groups (P < 0.05). The enamel covered by GQDs and GO showed better crystal structure formation and less mineral loss (P < 0.05) than that covered by CPP-ACP alone. After 7 days in the biofilm-challenged environment, the GO@CPP-ACP group showed less lesion depth than the CPP-ACP and control groups (P < 0.05). GO and GQDs showed good biocompatibility compared to the control group by CCK8 (P > 0.05) within 3 days. ConclusionGO and GQDs could improve the anti-caries effects of CPP-ACP, and CPP-ACP agents with GO or GQDs could be a potential option for enamel lesion management.Clinical Significance: GO and GQDs have demonstrated the potential to significantly enhance the anticaries effects of CPP-ACP. Incorporating these nanomaterials into CPP-ACP formulations could provide innovative and effective options for the management of enamel lesions, offering improved preventive and therapeutic strategies in dental care.

Assessment of the Effectiveness of Desensitizing Dentifrices on Management of Dental Hypersensitivity: An In Vitro Study.

The current study aimed to assess the efficiency of two desensitizing dentifrices on the management of dental hypersensitivity. For the purpose of this investigation, 60 extracted human sound premolar teeth that were removed for orthodontic purposes were collected. On the buccal cervical areas, an inverted-cone bur was used to create cavities that were 2 mm deep and 3 mm wide. The blocks were submerged in 17% ethylenediaminetetraacetic acid (EDTA) for 40 minutes in order to ensure the complete dentin tubule opening. Following preparation, all samples were split into three groups, each containing 20 samples, Group A: Control, Group B: Dentifrice containing calcium sodium phosphosilicate, Group C: Dentifrice containing casein phosphopeptide-amorphous calcium phosphate (CPP-ACP). Scanning electron microscopy (SEM) was used to assess the occlusion of dentinal tubules. One-way analysis of variance (ANOVA) was used to assess the desensitization efficacy of dentifrices. At a p-value less than 0.05, statistical significance was determined. Before application of different dentifrices, the maximum dentinal tubules opened in dentifrice containing CPP-ACP group (4.24 ± 0.10) followed by control group (4.18 ± 0.01) and dentifrice containing calcium sodium phosphosilicate (4.12 ± 0.06). And there was no significant difference between the different dentifrice groups (p > 0.001). After application of different dentifrices, the highest occlusion of dentinal tubules found in dentifrice containing CPP-ACP group (2.50 ± 0.05) followed by dentifrice containing calcium sodium phosphosilicate (2.84 ± 0.10) and control group (4.02 ± 0.07) and there was a highly significant difference between the different dentifrice groups (p < 0.001). On conclusion, dentifrice containing CPP-ACP exhibited the highest level of dentinal tubule occlusion in comparison to the control group and dentifrice containing calcium sodium phosphosilicate. Dentinal hypersensitivity (DH) is a condition that is frequently experienced. With variable outcomes, a number of products are utilized in the management of DH. Need is felt in dentistry for a material that chemically reacts, physically occludes and adheres intimately to dentinal tubules to reduce the possibility of its recurrence. How to cite this article: Pulipaka S, Ramanna PK, Samson A, et al. Assessment of the Effectiveness of Desensitizing Dentifrices on Management of Dental Hypersensitivity: An In Vitro Study. J Contemp Dent Pract 2024;25(5):494-497.

The Effect of Casein Phosphopeptide-amorphous Calcium Phosphate after Bleaching on the Shear Bond Strength of Ceramic Brackets

The Effect of Casein Phosphopeptide-amorphous Calcium Phosphate after Bleaching on the Shear Bond Strength of Ceramic Brackets

Evaluating the Remineralizing Effects of Calcium Sucrose Phosphate and Casein Phosphopeptide-Amorphous Calcium Phosphate Toothpastes on Artificial Carious Lesions Using Micro-computed Tomography: An In Vitro Investigation.

Background White spot lesions (WSLs) are common early indicators of enamel demineralization, particularly in pediatric orthodontic patients. Effective remineralization of these lesions is crucial for preventing further dental decay. This study aimed to evaluate the three-dimensional remineralization efficacy of two commercial toothpastes, calcium sucrose phosphate (CaSP) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), using micro-computed tomography (micro-CT). Objectives To compare the remineralization efficacy of CaSP and CPP-ACP on artificially created WSLs in human premolar enamel using micro-CT assessment. Materials and methods Freshly extracted, caries-free human premolars were used for the purpose of the study. Teeth with any defects, including caries, non-carious lesions, fractures, or hypocalcifications, were excluded. Teeth were stored in a saline solution with 0.1% thymol at +4°C until experimentation. Enamel slabs (N = 18) of 3 x 3 x 1.5 mm were prepared from the buccal surfaces of the premolars and polished to a mirror-like finish. The slabs were divided into three groups (n=6 each): control, CaSP, and CPP-ACP. Specimens were demineralized in a demineralization solution for 72 hours and then treated with the respective toothpastes or remineralizing solutions for 13 days. Micro-CT scanning was performed to assess changes in enamel volume and mineral density. Statistical analysis included Shapiro-Wilk tests, paired t-tests, and one-way ANOVA with post-hoc Tukey's HSD tests. Results Enamel volume changes were significant between groups (p<0.01), with the CaSP group showing the largest remineralization effect. Enamel mineral density changes were also significant (p=0.004), with the CPP-ACP group showing the greatest improvement in mineral density. Conclusions CaSPand CPP-ACPare both effective in remineralizing artificial enamel lesions. While CaSP shows comparable efficacy to CPP-ACP, further research is needed to confirm these findings in clinical settings. CaSP pastecan be considered a viable, cost-effective alternative for enamel remineralization.

Remineralization efficiency of three different agents on artificially produced enamel lesions: A micro-CT study.

This study aimed to assess the remineralization efficacy of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), silver diamine fluoride/potassium iodide (SDF/KI), and sodium fluoride with functionalized tricalcium phosphate (NaF/fTCP) on artificial early enamel lesion using laser fluorescence and micro-CT analysis. On extracted impacted third molars, artificial enamel lesions were prepared. Twenty-eight specimens were randomly assigned to four groups (n = 7 per group): a control group (artificial saliva), CPP-ACP (GC Tooth Mousse), SDF/KI (Riva Star), and NaF/fTCP (Clinpro White varnish). Following the manufacturer's instructions, the remineralization agents were applied to demineralized surfaces. Laser fluorescence and micro-CT were used to evaluate the remineralization efficacy of the agents and analyzes were performed during four stages: before demineralization, after demineralization, 1st day of remineralization and 30th day of remineralization. Shapiro-Wilk test, repeated measures two-way ANOVA, and Spearman correlation tests were used for statistical analysis. A significant level of p < 0.05 was established. SDF/KI significantly reduced the lesion area and lesion volume on the demineralized enamel surface after 30 days of remineralization. In the T3 period, SDF/KI increased the mineral density statistically significantly compared to the T1 period. The laser fluorescence values for all three remineralizing agents exhibited a linear decrease. A significant correlation between the fluorescence values and the mineral density was found (p = 0.01). All three investigated agents were showed positive remineralization efficacy on artificial enamel lesion. However, SDF/KI, containing silver diamine fluoride and potassium iodide exhibited superior than other agents in promoting remineralization. Although all three remineralization agents showed positive remineralization efficacy on artificial enamel lesions, SDF had higher remineralization performance over the other two agents. SDF has potential to prevent progression of demineralization in treating children with high caries risk in the long-term.

Evaluation Of The Effect Of Current Remineralization Agents On Enamel By Different Methods

Objectives: In this study, it was aimed to quantitatively evaluate the remineralization capacity of natural medicaments such as ginger, grape seed extract and rosemary, and materials containing casein and hydroxyapatite of initial caries lesions in vitro.. Materials and Methods: 42 enamel samples obtained from human teeth; were divided into six group as; Group-I (10% Casein Phosphopeptide Amorphous Calcium Phosphate [CPP-ACP], GC Tooth Mousse, Group-II (Rosemary Oil), Group-III (ginger+honey), Group-IV (ginger+honey+cocoa), Group-V (grape seed extract) and Group-VI (control group; remineralization solution). After enamel samples were kept in demineralization solution for 72 hours to create initial caries lesion, grouped remineralization agents were applied to enamel samples with caries lesion during the six-day pH cycle. Measurements were made using surface microhardness and DIAGNOdent at three different stages. The data obtained in the research were analyzed using the SPSS 25.0 program. The results were evaluated at the p ginger+honey+cocoa&gt;ginger+honey&gt; rosemary oil &gt; GC tooth mousse. Conclusions: : It is thought that rosemary, ginger + honey + cocoa, grape seed extract materials can be used for remineralization in these working conditions where the initial caries lesions can be remineralized after six days.